AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042203.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042203.01.T01 MTR_8g012210 96.188 341 13 0 2 342 30 370 0.0 678
MsG0880042203.01.T01 MTR_2g437030 23.024 291 211 8 35 320 172 454 3.93e-13 70.5
MsG0880042203.01.T01 MTR_2g437260 20.130 308 233 9 35 335 80 381 1.49e-12 68.6
MsG0880042203.01.T01 MTR_4g084630 21.951 246 182 4 35 270 17 262 4.53e-12 66.6
MsG0880042203.01.T01 MTR_2g437020 21.242 306 228 7 6 305 57 355 4.54e-12 67.0
MsG0880042203.01.T01 MTR_2g436400 20.139 288 225 4 34 320 92 375 6.99e-12 66.2
MsG0880042203.01.T01 MTR_4g120380 21.359 309 224 9 19 319 72 369 7.49e-12 66.2
MsG0880042203.01.T01 MTR_2g437080 22.260 292 214 7 34 320 85 368 8.89e-12 65.9
MsG0880042203.01.T01 MTR_2g019810 23.052 308 169 11 30 323 107 360 1.35e-11 65.5
MsG0880042203.01.T01 MTR_2g437160 21.493 335 234 11 19 342 93 409 2.05e-11 65.1
MsG0880042203.01.T01 MTR_6g083110 56.364 55 20 1 244 298 1 51 2.39e-11 61.6
MsG0880042203.01.T01 MTR_2g436440 22.337 291 213 7 35 320 92 374 3.15e-11 64.3
MsG0880042203.01.T01 MTR_2g437960 21.812 298 219 9 35 325 99 389 3.47e-11 64.3
MsG0880042203.01.T01 MTR_2g437960 21.812 298 219 9 35 325 99 389 3.93e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042203.01.T01 AT1G79220 48.936 329 159 4 21 342 73 399 7.87e-106 316
MsG0880042203.01.T01 AT1G61990 21.515 330 204 9 34 321 94 410 6.59e-12 66.6
MsG0880042203.01.T01 AT1G61990 21.515 330 204 9 34 321 94 410 6.59e-12 66.6
MsG0880042203.01.T01 AT5G64950 25.090 279 183 7 59 320 107 376 2.22e-11 65.1
MsG0880042203.01.T01 AT1G21150 21.667 240 181 4 35 269 63 300 2.59e-11 64.7
MsG0880042203.01.T01 AT1G21150 21.429 238 184 3 35 269 99 336 3.02e-11 64.3
MsG0880042203.01.T01 AT1G62010 20.544 331 217 9 29 319 75 399 4.96e-11 63.9
MsG0880042203.01.T01 AT1G21150 21.429 238 184 3 35 269 163 400 5.75e-11 63.9

Find 57 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCGCTTGCTTAGTGATT+TGG 0.240350 8:-5319721 MsG0880042203.01.T01:CDS
CTTCGCTTGCTTAGTGATTT+GGG 0.268010 8:-5319720 MsG0880042203.01.T01:CDS
ATTGACAATGTCAAAATATT+AGG 0.269638 8:+5319240 None:intergenic
GGAAATTGCTGATGAGTTAA+TGG 0.297355 8:-5318980 MsG0880042203.01.T01:CDS
TTGCTTAGTGATTTGGGTTT+AGG 0.299185 8:-5319714 MsG0880042203.01.T01:CDS
AAATATGTGGTGACATTAAT+AGG 0.301744 8:-5319357 MsG0880042203.01.T01:CDS
TAGCAGTTTCATCAAGGAAT+TGG 0.311012 8:-5318924 MsG0880042203.01.T01:CDS
CAAGCCTCTCGTCGAAATTA+TGG 0.312035 8:+5319631 None:intergenic
AGCAATGAAGGTACAGAATA+TGG 0.327499 8:-5319091 MsG0880042203.01.T01:CDS
TTGTAAAGATTATCAATTGC+AGG 0.347721 8:-5319680 MsG0880042203.01.T01:CDS
ATATTTGAAAACAACAGATA+TGG 0.357754 8:+5319144 None:intergenic
TTTGTACAATTTCGAATCCT+TGG 0.360192 8:+5319377 None:intergenic
TTTGACATTGTCAATTGATA+AGG 0.373162 8:-5319232 MsG0880042203.01.T01:CDS
TTCATTGCTAGAAGCACTCT+TGG 0.407140 8:+5319105 None:intergenic
TATGTCATTGTTTGAGTCAA+AGG 0.408420 8:-5319601 MsG0880042203.01.T01:CDS
CATCAGCAATTTCCTTATCA+TGG 0.411377 8:+5318989 None:intergenic
GTTGCACAGTATGAAGAGTT+AGG 0.413445 8:-5319507 MsG0880042203.01.T01:CDS
ATGGAATATATAAGTAGGAT+TGG 0.421574 8:-5319405 MsG0880042203.01.T01:CDS
GGGTTGCGGTGACGACGATT+TGG 0.427364 8:-5319805 MsG0880042203.01.T01:CDS
GCGCAATGCAGATCCTTCTC+AGG 0.427960 8:-5319754 MsG0880042203.01.T01:CDS
TGTGGAAACAATTCGCGAAA+AGG 0.448608 8:-5319325 MsG0880042203.01.T01:CDS
CCTCAATGCTCTAAGTATTG+AGG 0.451275 8:+5319050 None:intergenic
CTCAATGCTCTAAGTATTGA+GGG 0.456627 8:+5319051 None:intergenic
TTCCTTTGACGATGAGAAGA+TGG 0.459653 8:-5319424 MsG0880042203.01.T01:CDS
AATTCAACCTCATTCTGAGC+TGG 0.463701 8:+5319879 None:intergenic
AAGAGTTAGGTATACCGAAA+AGG 0.464523 8:-5319494 MsG0880042203.01.T01:CDS
GTCAAAGGAAATGCTTCATA+AGG 0.473131 8:-5319586 MsG0880042203.01.T01:CDS
AGCTGGGACTTTCGTCGAAT+AGG 0.477720 8:+5319896 None:intergenic
TGGAATATATAAGTAGGATT+GGG 0.487565 8:-5319404 MsG0880042203.01.T01:CDS
AGAGTTAGGTATACCGAAAA+GGG 0.502937 8:-5319493 MsG0880042203.01.T01:CDS
GTTTGTCAGATGCCATGATA+AGG 0.508257 8:-5319001 MsG0880042203.01.T01:CDS
AGGTATACCGAAAAGGGACT+TGG 0.508376 8:-5319487 MsG0880042203.01.T01:CDS
TATTTGAAAACAACAGATAT+GGG 0.512551 8:+5319145 None:intergenic
CATATAGAAGTAGTTCAGAA+AGG 0.515005 8:+5318886 None:intergenic
GGATCTGCATTGCGCAAAGA+CGG 0.515146 8:+5319762 None:intergenic
AAATGCTAAAGACAAATTCA+AGG 0.522593 8:-5319853 MsG0880042203.01.T01:CDS
GATCTGCATTGCGCAAAGAC+GGG 0.526314 8:+5319763 None:intergenic
AGAGATTAGCAGTTTCATCA+AGG 0.527319 8:-5318930 MsG0880042203.01.T01:CDS
AAGAGTGCTTCTAGCAATGA+AGG 0.527541 8:-5319103 MsG0880042203.01.T01:CDS
CAATGAAGGGTTTCTTGCAA+TGG 0.534493 8:+5319563 None:intergenic
ATATAGAAGTAGTTCAGAAA+GGG 0.534546 8:+5318887 None:intergenic
AGAAGATGGAATATATAAGT+AGG 0.535407 8:-5319410 MsG0880042203.01.T01:CDS
ATTCAACCTCATTCTGAGCT+GGG 0.539471 8:+5319880 None:intergenic
TTCGAAATTGTACAAATATG+TGG 0.547821 8:-5319370 MsG0880042203.01.T01:CDS
AAGTAGGATTGGGCTTTCCA+AGG 0.560292 8:-5319394 MsG0880042203.01.T01:CDS
TTCCATCTTCTCATCGTCAA+AGG 0.562047 8:+5319422 None:intergenic
TGTCAATTGATAAGGTTCAA+AGG 0.565097 8:-5319224 MsG0880042203.01.T01:CDS
TCAAGGAATTGGACTACTAG+AGG 0.573509 8:-5318913 MsG0880042203.01.T01:CDS
CATTGAGGATGTCCGAGGAG+AGG 0.582695 8:-5319035 MsG0880042203.01.T01:CDS
ACTTTCGTCGAATAGGTGGC+AGG 0.582772 8:+5319903 None:intergenic
ATTAATAGGCATCTCTCGTG+TGG 0.591058 8:-5319343 MsG0880042203.01.T01:CDS
CCTCAATACTTAGAGCATTG+AGG 0.607754 8:-5319050 MsG0880042203.01.T01:CDS
TGGGACTTTCGTCGAATAGG+TGG 0.610226 8:+5319899 None:intergenic
GAAAGTCCCAGCTCAGAATG+AGG 0.610873 8:-5319886 MsG0880042203.01.T01:CDS
TTAACCATAATTTCGACGAG+AGG 0.625796 8:-5319635 MsG0880042203.01.T01:CDS
GAAGTTCTTGAAATAACCGT+CGG 0.665623 8:+5319444 None:intergenic
TAGAGCATTGAGGATGTCCG+AGG 0.681763 8:-5319040 MsG0880042203.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTAATGGAATTTTATAAA+AGG - Chr8:5319840-5319859 MsG0880042203.01.T01:CDS 15.0%
!! ATATTTGAAAACAACAGATA+TGG + Chr8:5319665-5319684 None:intergenic 20.0%
!! ATTGACAATGTCAAAATATT+AGG + Chr8:5319569-5319588 None:intergenic 20.0%
!! TATTTGAAAACAACAGATAT+GGG + Chr8:5319664-5319683 None:intergenic 20.0%
!!! ATATCTGTTGTTTTCAAATA+TGG - Chr8:5319664-5319683 MsG0880042203.01.T01:CDS 20.0%
!!! ATTTTATAAAAGGACAAAAC+AGG - Chr8:5319850-5319869 MsG0880042203.01.T01:CDS 20.0%
!!! TTTTACAAAGTATGGTTTTT+CGG - Chr8:5319511-5319530 MsG0880042203.01.T01:CDS 20.0%
! AAATATGTGGTGACATTAAT+AGG - Chr8:5319449-5319468 MsG0880042203.01.T01:CDS 25.0%
! AAATGCTAAAGACAAATTCA+AGG - Chr8:5318953-5318972 MsG0880042203.01.T01:CDS 25.0%
! AGAAGATGGAATATATAAGT+AGG - Chr8:5319396-5319415 MsG0880042203.01.T01:CDS 25.0%
! ATGGAATATATAAGTAGGAT+TGG - Chr8:5319401-5319420 MsG0880042203.01.T01:CDS 25.0%
! TGGAATATATAAGTAGGATT+GGG - Chr8:5319402-5319421 MsG0880042203.01.T01:CDS 25.0%
! TTCGAAATTGTACAAATATG+TGG - Chr8:5319436-5319455 MsG0880042203.01.T01:CDS 25.0%
! TTGTAAAGATTATCAATTGC+AGG - Chr8:5319126-5319145 MsG0880042203.01.T01:CDS 25.0%
! TTTGACATTGTCAATTGATA+AGG - Chr8:5319574-5319593 MsG0880042203.01.T01:CDS 25.0%
!! ATGAAATATTCGGTCTTTTT+GGG - Chr8:5319537-5319556 MsG0880042203.01.T01:CDS 25.0%
!! GTGTTGAATTTTACAAAGTA+TGG - Chr8:5319503-5319522 MsG0880042203.01.T01:CDS 25.0%
!! TTTTCGGATGATGAAATATT+CGG - Chr8:5319527-5319546 MsG0880042203.01.T01:CDS 25.0%
!!! TACTGAAGTTTTGAGTAAAT+GGG - Chr8:5318980-5318999 MsG0880042203.01.T01:CDS 25.0%
TATGTCATTGTTTGAGTCAA+AGG - Chr8:5319205-5319224 MsG0880042203.01.T01:CDS 30.0%
TGTCAATTGATAAGGTTCAA+AGG - Chr8:5319582-5319601 MsG0880042203.01.T01:CDS 30.0%
TTTGTACAATTTCGAATCCT+TGG + Chr8:5319432-5319451 None:intergenic 30.0%
! AGGAACATGACTTTCATTTT+AGG - Chr8:5319602-5319621 MsG0880042203.01.T01:CDS 30.0%
! GATGAAATATTCGGTCTTTT+TGG - Chr8:5319536-5319555 MsG0880042203.01.T01:CDS 30.0%
! TATTTTCACACAGCAATGAA+GGG + Chr8:5319259-5319278 None:intergenic 30.0%
! TGGTTCAAATGATGATTTTG+CGG - Chr8:5319339-5319358 MsG0880042203.01.T01:CDS 30.0%
! TTATTTTCACACAGCAATGA+AGG + Chr8:5319260-5319279 None:intergenic 30.0%
!!! ACTGAAGTTTTGAGTAAATG+GGG - Chr8:5318981-5319000 MsG0880042203.01.T01:CDS 30.0%
!!! ATTGAGGGTGTTTTCATATT+AGG + Chr8:5319743-5319762 None:intergenic 30.0%
!!! CTACTGAAGTTTTGAGTAAA+TGG - Chr8:5318979-5318998 MsG0880042203.01.T01:CDS 30.0%
AAGAGTTAGGTATACCGAAA+AGG - Chr8:5319312-5319331 MsG0880042203.01.T01:CDS 35.0%
AGAGATTAGCAGTTTCATCA+AGG - Chr8:5319876-5319895 MsG0880042203.01.T01:CDS 35.0%
AGAGTTAGGTATACCGAAAA+GGG - Chr8:5319313-5319332 MsG0880042203.01.T01:CDS 35.0%
CATCAGCAATTTCCTTATCA+TGG + Chr8:5319820-5319839 None:intergenic 35.0%
GAAGTTCTTGAAATAACCGT+CGG + Chr8:5319365-5319384 None:intergenic 35.0%
GGAAATTGCTGATGAGTTAA+TGG - Chr8:5319826-5319845 MsG0880042203.01.T01:CDS 35.0%
GTCAAAGGAAATGCTTCATA+AGG - Chr8:5319220-5319239 MsG0880042203.01.T01:CDS 35.0%
TAGCAGTTTCATCAAGGAAT+TGG - Chr8:5319882-5319901 MsG0880042203.01.T01:CDS 35.0%
TTAACCATAATTTCGACGAG+AGG - Chr8:5319171-5319190 MsG0880042203.01.T01:CDS 35.0%
! AGCAATGAAGGTACAGAATA+TGG - Chr8:5319715-5319734 MsG0880042203.01.T01:CDS 35.0%
! ATTTGAACCAAGTCCCTTTT+CGG + Chr8:5319329-5319348 None:intergenic 35.0%
! TTGCTTAGTGATTTGGGTTT+AGG - Chr8:5319092-5319111 MsG0880042203.01.T01:CDS 35.0%
!! CTCAATGCTCTAAGTATTGA+GGG + Chr8:5319758-5319777 None:intergenic 35.0%
AATTCAACCTCATTCTGAGC+TGG + Chr8:5318930-5318949 None:intergenic 40.0%
ACTTCGCTTGCTTAGTGATT+TGG - Chr8:5319085-5319104 MsG0880042203.01.T01:CDS 40.0%
ATTAATAGGCATCTCTCGTG+TGG - Chr8:5319463-5319482 MsG0880042203.01.T01:CDS 40.0%
ATTCAACCTCATTCTGAGCT+GGG + Chr8:5318929-5318948 None:intergenic 40.0%
CAATGAAGGGTTTCTTGCAA+TGG + Chr8:5319246-5319265 None:intergenic 40.0%
CGTATTGAAAAACCTCTCCT+CGG + Chr8:5319786-5319805 None:intergenic 40.0%
CTTCGCTTGCTTAGTGATTT+GGG - Chr8:5319086-5319105 MsG0880042203.01.T01:CDS 40.0%
GTTGCACAGTATGAAGAGTT+AGG - Chr8:5319299-5319318 MsG0880042203.01.T01:CDS 40.0%
GTTTGTCAGATGCCATGATA+AGG - Chr8:5319805-5319824 MsG0880042203.01.T01:CDS 40.0%
TCAAGGAATTGGACTACTAG+AGG - Chr8:5319893-5319912 MsG0880042203.01.T01:CDS 40.0%
TGTGGAAACAATTCGCGAAA+AGG - Chr8:5319481-5319500 MsG0880042203.01.T01:CDS 40.0%
TTCCATCTTCTCATCGTCAA+AGG + Chr8:5319387-5319406 None:intergenic 40.0%
TTCCTTTGACGATGAGAAGA+TGG - Chr8:5319382-5319401 MsG0880042203.01.T01:CDS 40.0%
! CCTCAATGCTCTAAGTATTG+AGG + Chr8:5319759-5319778 None:intergenic 40.0%
!! AAGAGTGCTTCTAGCAATGA+AGG - Chr8:5319703-5319722 MsG0880042203.01.T01:CDS 40.0%
!! CCTCAATACTTAGAGCATTG+AGG - Chr8:5319756-5319775 MsG0880042203.01.T01:CDS 40.0%
!! GTTTTGAGTAAATGGGGTTG+CGG - Chr8:5318987-5319006 MsG0880042203.01.T01:CDS 40.0%
!! TTCATTGCTAGAAGCACTCT+TGG + Chr8:5319704-5319723 None:intergenic 40.0%
!!! AGTTTTGACTGAACCTGAGA+AGG + Chr8:5319068-5319087 None:intergenic 40.0%
AAGTAGGATTGGGCTTTCCA+AGG - Chr8:5319412-5319431 MsG0880042203.01.T01:CDS 45.0%
AGGTATACCGAAAAGGGACT+TGG - Chr8:5319319-5319338 MsG0880042203.01.T01:CDS 45.0%
AGTCGAGTCCTGAAAAATCG+AGG + Chr8:5319152-5319171 None:intergenic 45.0%
CAAGCCTCTCGTCGAAATTA+TGG + Chr8:5319178-5319197 None:intergenic 45.0%
! AATGATGATTTTGCGGCCGA+CGG - Chr8:5319346-5319365 MsG0880042203.01.T01:CDS 45.0%
! ATTGCAGGCCTCGATTTTTC+AGG - Chr8:5319141-5319160 MsG0880042203.01.T01:CDS 45.0%
ACTTTCGTCGAATAGGTGGC+AGG + Chr8:5318906-5318925 None:intergenic 50.0%
AGCTGGGACTTTCGTCGAAT+AGG + Chr8:5318913-5318932 None:intergenic 50.0%
GAAAGTCCCAGCTCAGAATG+AGG - Chr8:5318920-5318939 MsG0880042203.01.T01:CDS 50.0%
GATCTGCATTGCGCAAAGAC+GGG + Chr8:5319046-5319065 None:intergenic 50.0%
GGATCTGCATTGCGCAAAGA+CGG + Chr8:5319047-5319066 None:intergenic 50.0%
TGGGACTTTCGTCGAATAGG+TGG + Chr8:5318910-5318929 None:intergenic 50.0%
!! TAGAGCATTGAGGATGTCCG+AGG - Chr8:5319766-5319785 MsG0880042203.01.T01:CDS 50.0%
CATTGAGGATGTCCGAGGAG+AGG - Chr8:5319771-5319790 MsG0880042203.01.T01:CDS 55.0%
GCGCAATGCAGATCCTTCTC+AGG - Chr8:5319052-5319071 MsG0880042203.01.T01:CDS 55.0%
GGGTTGCGGTGACGACGATT+TGG - Chr8:5319001-5319020 MsG0880042203.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 5318900 5319928 5318900 ID=MsG0880042203.01;Name=MsG0880042203.01
Chr8 mRNA 5318900 5319928 5318900 ID=MsG0880042203.01.T01;Parent=MsG0880042203.01;Name=MsG0880042203.01.T01;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|342
Chr8 exon 5318900 5319928 5318900 ID=MsG0880042203.01.T01:exon:1252;Parent=MsG0880042203.01.T01
Chr8 CDS 5318900 5319928 5318900 ID=MsG0880042203.01.T01:cds;Parent=MsG0880042203.01.T01
Gene Sequence

>MsG0880042203.01.T01

ATGCCTGCCACCTATTCGACGAAAGTCCCAGCTCAGAATGAGGTTGAATTGCAAAATGCTAAAGACAAATTCAAGGATGCTACTGAAGTTTTGAGTAAATGGGGTTGCGGTGACGACGATTTGGCAAGAATATTCACTCGTTGCCCGTCTTTGCGCAATGCAGATCCTTCTCAGGTTCAGTCAAAACTTCGCTTGCTTAGTGATTTGGGTTTAGGCTCAGCTGAGCTTGTAAAGATTATCAATTGCAGGCCTCGATTTTTCAGGACTCGACTTAACCATAATTTCGACGAGAGGCTTGACTCTCTTATGTCATTGTTTGAGTCAAAGGAAATGCTTCATAAGGCCATTGCAAGAAACCCTTCATTGCTGTGTGAAAATAATTATGACATTGAACGTATTGTTGCACAGTATGAAGAGTTAGGTATACCGAAAAGGGACTTGGTTCAAATGATGATTTTGCGGCCGACGGTTATTTCAAGAACTTCCTTTGACGATGAGAAGATGGAATATATAAGTAGGATTGGGCTTTCCAAGGATTCGAAATTGTACAAATATGTGGTGACATTAATAGGCATCTCTCGTGTGGAAACAATTCGCGAAAAGGTGTTGAATTTTACAAAGTATGGTTTTTCGGATGATGAAATATTCGGTCTTTTTGGGAAGTCTCCTAATATTTTGACATTGTCAATTGATAAGGTTCAAAGGAACATGACTTTCATTTTAGGCACAATGAAGCTTGAAGCTAACATAATCTTTACGTACCCATATCTGTTGTTTTCAAATATGGAGACTGTTTTGAAGCCAAGAGTGCTTCTAGCAATGAAGGTACAGAATATGGTTCCTAATATGAAAACACCCTCAATACTTAGAGCATTGAGGATGTCCGAGGAGAGGTTTTTCAATACGTTTGTCAGATGCCATGATAAGGAAATTGCTGATGAGTTAATGGAATTTTATAAAAGGACAAAACAGGTTAAGAGATTAGCAGTTTCATCAAGGAATTGGACTACTAGAGGATTCCCTTTCTGA

Protein sequence

>MsG0880042203.01.T01

MPATYSTKVPAQNEVELQNAKDKFKDATEVLSKWGCGDDDLARIFTRCPSLRNADPSQVQSKLRLLSDLGLGSAELVKIINCRPRFFRTRLNHNFDERLDSLMSLFESKEMLHKAIARNPSLLCENNYDIERIVAQYEELGIPKRDLVQMMILRPTVISRTSFDDEKMEYISRIGLSKDSKLYKYVVTLIGISRVETIREKVLNFTKYGFSDDEIFGLFGKSPNILTLSIDKVQRNMTFILGTMKLEANIIFTYPYLLFSNMETVLKPRVLLAMKVQNMVPNMKTPSILRALRMSEERFFNTFVRCHDKEIADELMEFYKRTKQVKRLAVSSRNWTTRGFPF*