AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042611.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042611.01.T01 MTR_8g018270 87.293 181 23 0 1 181 1054 1234 4.90e-88 280
MsG0880042611.01.T01 MTR_8g018280 75.000 180 45 0 2 181 1294 1473 9.60e-75 244
MsG0880042611.01.T01 MTR_5g086690 73.256 172 46 0 2 173 1308 1479 6.57e-70 230
MsG0880042611.01.T01 MTR_8g018160 71.111 45 13 0 25 69 1 45 7.33e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCATCAGCTCTTTGCTTT+CGG 0.279935 8:+11458905 None:intergenic
CAGCGGCAATTACATGTCTT+TGG 0.293847 8:-11459321 None:intergenic
CAATAGAGGATGTCATATTA+TGG 0.331248 8:+11459243 None:intergenic
TCAACTTCTGCTGCTTTGAC+AGG 0.334818 8:+11458847 None:intergenic
ATAGAATTTGCAGACAAGTA+TGG 0.363847 8:-11458801 MsG0880042611.01.T01:CDS
TGGACAAGCTTCACAACATT+AGG 0.371469 8:+11459263 None:intergenic
AGCAGAAGTTGAAAGACAAA+TGG 0.425002 8:-11458836 MsG0880042611.01.T01:CDS
GCAAAAGAATATTCTTGAGT+TGG 0.430621 8:-11459009 MsG0880042611.01.T01:CDS
GAAAGAAAAGAGTCAGTTTG+AGG 0.447261 8:-11459096 MsG0880042611.01.T01:CDS
TGAAAGTGAAAAGAAGAAAG+TGG 0.451582 8:-11458952 MsG0880042611.01.T01:CDS
GCAGAAGTTGAAAGACAAAT+GGG 0.480276 8:-11458835 MsG0880042611.01.T01:CDS
ATTGAAAAGGTTCTCAATAG+AGG 0.496397 8:+11459229 None:intergenic
CAACTTCTGCTGCTTTGACA+GGG 0.502838 8:+11458848 None:intergenic
CCATGACGAAGTCATTGAAA+AGG 0.508796 8:+11459216 None:intergenic
AACAAAGTTGAAGGAGAGCA+TGG 0.522533 8:-11458982 MsG0880042611.01.T01:CDS
AGCTAAGCGTGTCGAGGAAG+AGG 0.527737 8:-11459069 MsG0880042611.01.T01:CDS
AAAGAAGAAAGTGGAAGATG+AGG 0.561565 8:-11458943 MsG0880042611.01.T01:CDS
TACCGAAAGCAAAGAGCTGA+TGG 0.564760 8:-11458907 MsG0880042611.01.T01:CDS
CGAGGAAGAGGTTGAAAAGA+CGG 0.567502 8:-11459057 MsG0880042611.01.T01:CDS
GACATATGCTAAGAAACAGA+AGG 0.610551 8:-11459129 MsG0880042611.01.T01:CDS
AAGTTGAAAGACAAATGGGA+AGG 0.630448 8:-11458831 MsG0880042611.01.T01:CDS
GGATGAAGCTAAGCGTGTCG+AGG 0.666115 8:-11459075 MsG0880042611.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATAGAATTTGCAGACAAGTA+TGG - Chr8:11459292-11459311 MsG0880042611.01.T01:CDS 30.0%
ATTGAAAAGGTTCTCAATAG+AGG + Chr8:11458867-11458886 None:intergenic 30.0%
CAATAGAGGATGTCATATTA+TGG + Chr8:11458853-11458872 None:intergenic 30.0%
GCAAAAGAATATTCTTGAGT+TGG - Chr8:11459084-11459103 MsG0880042611.01.T01:CDS 30.0%
GTTGGAAAAAACAAAGTTGA+AGG - Chr8:11459102-11459121 MsG0880042611.01.T01:CDS 30.0%
TGAAAGTGAAAAGAAGAAAG+TGG - Chr8:11459141-11459160 MsG0880042611.01.T01:CDS 30.0%
AAAGAAGAAAGTGGAAGATG+AGG - Chr8:11459150-11459169 MsG0880042611.01.T01:CDS 35.0%
AAGTTGAAAGACAAATGGGA+AGG - Chr8:11459262-11459281 MsG0880042611.01.T01:CDS 35.0%
AGCAGAAGTTGAAAGACAAA+TGG - Chr8:11459257-11459276 MsG0880042611.01.T01:CDS 35.0%
GAAAGAAAAGAGTCAGTTTG+AGG - Chr8:11458997-11459016 MsG0880042611.01.T01:CDS 35.0%
GACATATGCTAAGAAACAGA+AGG - Chr8:11458964-11458983 MsG0880042611.01.T01:CDS 35.0%
GCAGAAGTTGAAAGACAAAT+GGG - Chr8:11459258-11459277 MsG0880042611.01.T01:CDS 35.0%
AACAAAGTTGAAGGAGAGCA+TGG - Chr8:11459111-11459130 MsG0880042611.01.T01:CDS 40.0%
CATGGCTAAAAATGTGGTTG+AGG - Chr8:11458895-11458914 MsG0880042611.01.T01:CDS 40.0%
CCATGACGAAGTCATTGAAA+AGG + Chr8:11458880-11458899 None:intergenic 40.0%
CTTCGTCATGGCTAAAAATG+TGG - Chr8:11458889-11458908 MsG0880042611.01.T01:CDS 40.0%
TGGACAAGCTTCACAACATT+AGG + Chr8:11458833-11458852 None:intergenic 40.0%
! CCTTTTCAATGACTTCGTCA+TGG - Chr8:11458877-11458896 MsG0880042611.01.T01:CDS 40.0%
CGAGGAAGAGGTTGAAAAGA+CGG - Chr8:11459036-11459055 MsG0880042611.01.T01:CDS 45.0%
TACCGAAAGCAAAGAGCTGA+TGG - Chr8:11459186-11459205 MsG0880042611.01.T01:CDS 45.0%
!! CAACTTCTGCTGCTTTGACA+GGG + Chr8:11459248-11459267 None:intergenic 45.0%
!! TCAACTTCTGCTGCTTTGAC+AGG + Chr8:11459249-11459268 None:intergenic 45.0%
CGCCATCAGCTCTTTGCTTT+CGG + Chr8:11459191-11459210 None:intergenic 50.0%
AGCTAAGCGTGTCGAGGAAG+AGG - Chr8:11459024-11459043 MsG0880042611.01.T01:CDS 55.0%
GGATGAAGCTAAGCGTGTCG+AGG - Chr8:11459018-11459037 MsG0880042611.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 11458785 11459330 11458785 ID=MsG0880042611.01;Name=MsG0880042611.01
Chr8 mRNA 11458785 11459330 11458785 ID=MsG0880042611.01.T01;Parent=MsG0880042611.01;Name=MsG0880042611.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|181
Chr8 exon 11458785 11459330 11458785 ID=MsG0880042611.01.T01:exon:3502;Parent=MsG0880042611.01.T01
Chr8 CDS 11458785 11459330 11458785 ID=MsG0880042611.01.T01:cds;Parent=MsG0880042611.01.T01
Gene Sequence

>MsG0880042611.01.T01

ATGTCTTTGGAATGCATAAAACAAGAATCTCATGAAAAAGTTTCTCCTAATGTTGTGAAGCTTGTCCATAATATGACATCCTCTATTGAGAACCTTTTCAATGACTTCGTCATGGCTAAAAATGTGGTTGAGGATCACAATAATGCTTTACAACAAAAAGAAAAGCTTATACAGCGAGTGACATATGCTAAGAAACAGAAGGAATCAAAACAGAAAGAAAAGAGTCAGTTTGAGGATGAAGCTAAGCGTGTCGAGGAAGAGGTTGAAAAGACGGATGAAAAAATTAGAATACTTATTGAGCAAAAGAATATTCTTGAGTTGGAAAAAACAAAGTTGAAGGAGAGCATGGAAAGATGTGAAAGTGAAAAGAAGAAAGTGGAAGATGAGGCTAAGAATATGATTACCGAAAGCAAAGAGCTGATGGCGAGTATTAAGAAGTCTAAATCTTCGTATACTGCTGCCCTGTCAAAGCAGCAGAAGTTGAAAGACAAATGGGAAGGTTTCAGAATAGAATTTGCAGACAAGTATGGAAGTAGCAGTAGCTGA

Protein sequence

>MsG0880042611.01.T01

MSLECIKQESHEKVSPNVVKLVHNMTSSIENLFNDFVMAKNVVEDHNNALQQKEKLIQRVTYAKKQKESKQKEKSQFEDEAKRVEEEVEKTDEKIRILIEQKNILELEKTKLKESMERCESEKKKVEDEAKNMITESKELMASIKKSKSSYTAALSKQQKLKDKWEGFRIEFADKYGSSSS*