AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0481110


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0481110 MtrunA17_Chr4g0067541 92.982 114 7 1 1 113 1 114 1.44e-66 196
Msa0481110 MtrunA17_Chr2g0295651 60.684 117 33 3 1 109 1 112 4.06e-38 125
Msa0481110 MtrunA17_Chr3g0115551 46.970 66 31 1 38 103 71 132 4.95e-15 66.6
Msa0481110 MtrunA17_Chr8g0344991 40.541 74 36 1 36 101 6 79 5.09e-13 60.8
Msa0481110 MtrunA17_Chr4g0070221 52.083 48 22 1 56 103 173 219 7.13e-12 59.7
Msa0481110 MtrunA17_Chr3g0124361 38.889 72 41 1 35 103 95 166 2.76e-11 57.8
Msa0481110 MtrunA17_Chr3g0138651 53.191 47 22 0 56 102 349 395 7.18e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0481110 AT5G49120.1 62.264 53 20 0 56 108 63 115 4.25e-21 82.4
Msa0481110 AT1G22160.1 48.276 58 30 0 53 110 70 127 3.21e-14 64.7
Msa0481110 AT4G39795.1 42.029 69 39 1 33 101 46 113 4.21e-13 61.2
Msa0481110 AT1G78020.1 53.488 43 20 0 61 103 89 131 8.75e-13 61.2
Msa0481110 AT5G47060.2 56.000 50 21 1 61 110 97 145 7.38e-12 59.3
Msa0481110 AT5G47060.1 56.000 50 21 1 61 110 97 145 7.38e-12 59.3
Msa0481110 AT4G17670.1 52.000 50 23 1 61 110 76 124 9.18e-11 55.8

Find 19 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAAACCATGGGTAAAAC+TGG 0.321231 3_3:-86863112 None:intergenic
AGAAAAGTTGAAACCTTAAA+AGG 0.340742 3_3:-86862647 None:intergenic
AACCTTAAAAGGAGAATGAT+AGG 0.405313 3_3:-86862636 None:intergenic
CACTGGAAATGTTGGGGTGG+GGG 0.412454 3_3:+86863056 Msa0481110:three_prime_UTR
TGCAGCAAGAAGCTCTTACC+TGG 0.449852 3_3:+86862686 Msa0481110:CDS
AGTAGTGACACTGGAAATGT+TGG 0.487421 3_3:+86863048 Msa0481110:exon
AAAGGAGAATGATAGGAAAG+TGG 0.494802 3_3:-86862629 None:intergenic
CCTACAATCCACGCTACAAA+AGG 0.495514 3_3:-86862967 None:intergenic
ACACTGGAAATGTTGGGGTG+GGG 0.537070 3_3:+86863055 Msa0481110:three_prime_UTR
ATCAGAGAATGAAATATGGT+TGG 0.543247 3_3:+86862470 Msa0481110:exon
GAATGAAATATGGTTGGTCT+TGG 0.548398 3_3:+86862476 Msa0481110:exon
GACACTGGAAATGTTGGGGT+GGG 0.558364 3_3:+86863054 Msa0481110:three_prime_UTR
TCTTGCTGCATAGAAAGCAT+TGG 0.559666 3_3:-86862673 None:intergenic
GTTGATTGTGCTAAGCATCA+TGG 0.561835 3_3:-86862591 None:intergenic
GTAGTGACACTGGAAATGTT+GGG 0.588242 3_3:+86863049 Msa0481110:exon
TACATGTAGATATCTTTCCC+AGG 0.593576 3_3:-86862704 None:intergenic
GCAGCAAGAAGCTCTTACCT+GGG 0.606797 3_3:+86862687 Msa0481110:CDS
TGACACTGGAAATGTTGGGG+TGG 0.614413 3_3:+86863053 Msa0481110:three_prime_UTR
TAGTGACACTGGAAATGTTG+GGG 0.702637 3_3:+86863050 Msa0481110:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTTTTGCTA+TGG + chr3_3:86863081-86863100 Msa0481110:three_prime_UTR 10.0%
!! AAAAATCAGAGAATGAAATA+TGG + chr3_3:86862466-86862485 Msa0481110:exon 20.0%
!!! ATATTACTCTTTTTCTTATG+TGG - chr3_3:86862524-86862543 None:intergenic 20.0%
!!! CTTTTAATTTTATCTCCTAT+AGG + chr3_3:86862787-86862806 Msa0481110:intron 20.0%
!!! ATGCATTTTTTTTTTCTTCT+TGG + chr3_3:86862826-86862845 Msa0481110:intron 20.0%
!!! GAATTTTGTATTAGTATATG+TGG + chr3_3:86862895-86862914 Msa0481110:intron 20.0%
!!! GAGTTATTTTTTTGTCTTAA+TGG + chr3_3:86862917-86862936 Msa0481110:intron 20.0%
! AGAAAAGTTGAAACCTTAAA+AGG - chr3_3:86862650-86862669 None:intergenic 25.0%
! GTAAGATCTATATATCCTAT+AGG - chr3_3:86862805-86862824 None:intergenic 25.0%
! CAATTAACATCAAAAGATCT+GGG - chr3_3:86862876-86862895 None:intergenic 25.0%
! TCAATTAACATCAAAAGATC+TGG - chr3_3:86862877-86862896 None:intergenic 25.0%
! TCTGATTTTTCTTTCTGTTG+TGG - chr3_3:86862456-86862475 None:intergenic 30.0%
ATCAGAGAATGAAATATGGT+TGG + chr3_3:86862470-86862489 Msa0481110:exon 30.0%
! TTCCTATCATTCTCCTTTTA+AGG + chr3_3:86862634-86862653 Msa0481110:CDS 30.0%
AACCTTAAAAGGAGAATGAT+AGG - chr3_3:86862639-86862658 None:intergenic 30.0%
! AATGCATGCTACACTTATTT+TGG - chr3_3:86862747-86862766 None:intergenic 30.0%
!!! ATCTGGGTATTTTTTCTGTA+AGG - chr3_3:86862860-86862879 None:intergenic 30.0%
!! GAATGAAATATGGTTGGTCT+TGG + chr3_3:86862476-86862495 Msa0481110:exon 35.0%
AAAGGAGAATGATAGGAAAG+TGG - chr3_3:86862632-86862651 None:intergenic 35.0%
TACATGTAGATATCTTTCCC+AGG - chr3_3:86862707-86862726 None:intergenic 35.0%
TACATGTACAAGTGAGTTCT+CGG + chr3_3:86862719-86862738 Msa0481110:intron 35.0%
!! TAGGTGCAAGCATATTTTAG+CGG + chr3_3:86862986-86863005 Msa0481110:CDS 35.0%
!! TTTTTGAAGAGTAGTGACAC+TGG + chr3_3:86863039-86863058 Msa0481110:exon 35.0%
GTTGATTGTGCTAAGCATCA+TGG - chr3_3:86862594-86862613 None:intergenic 40.0%
! TCTTGCTGCATAGAAAGCAT+TGG - chr3_3:86862676-86862695 None:intergenic 40.0%
ATGGCACCTATTTATTGCAG+AGG + chr3_3:86862936-86862955 Msa0481110:intron 40.0%
CTGTCTCCTCTGCAATAAAT+AGG - chr3_3:86862945-86862964 None:intergenic 40.0%
AGTAGTGACACTGGAAATGT+TGG + chr3_3:86863048-86863067 Msa0481110:exon 40.0%
GTAGTGACACTGGAAATGTT+GGG + chr3_3:86863049-86863068 Msa0481110:exon 40.0%
TAGTGACACTGGAAATGTTG+GGG + chr3_3:86863050-86863069 Msa0481110:exon 40.0%
CCTACAATCCACGCTACAAA+AGG - chr3_3:86862970-86862989 None:intergenic 45.0%
!! CCTTTTGTAGCGTGGATTGT+AGG + chr3_3:86862967-86862986 Msa0481110:CDS 45.0%
!!! CAAGCATATTTTAGCGGACG+AGG + chr3_3:86862992-86863011 Msa0481110:CDS 45.0%
!!! TATTTTAGCGGACGAGGAAG+AGG + chr3_3:86862998-86863017 Msa0481110:CDS 45.0%
TGCAGCAAGAAGCTCTTACC+TGG + chr3_3:86862686-86862705 Msa0481110:CDS 50.0%
GCAGCAAGAAGCTCTTACCT+GGG + chr3_3:86862687-86862706 Msa0481110:CDS 50.0%
! AGACAGAGCCTTTTGTAGCG+TGG + chr3_3:86862959-86862978 Msa0481110:CDS 50.0%
TGACACTGGAAATGTTGGGG+TGG + chr3_3:86863053-86863072 Msa0481110:three_prime_UTR 50.0%
GACACTGGAAATGTTGGGGT+GGG + chr3_3:86863054-86863073 Msa0481110:three_prime_UTR 50.0%
ACACTGGAAATGTTGGGGTG+GGG + chr3_3:86863055-86863074 Msa0481110:three_prime_UTR 50.0%
CACTGGAAATGTTGGGGTGG+GGG + chr3_3:86863056-86863075 Msa0481110:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 86862439 86863121 86862439 ID=Msa0481110;Name=Msa0481110
chr3_3 mRNA 86862439 86863121 86862439 ID=Msa0481110-mRNA-1;Parent=Msa0481110;Name=Msa0481110-mRNA-1;_AED=0.00;_eAED=0.00;_QI=46|1|1|1|1|1|2|69|113
chr3_3 exon 86862439 86862729 86862439 ID=Msa0481110-mRNA-1:exon:25091;Parent=Msa0481110-mRNA-1
chr3_3 exon 86862956 86863121 86862956 ID=Msa0481110-mRNA-1:exon:25092;Parent=Msa0481110-mRNA-1
chr3_3 five_prime_UTR 86862439 86862484 86862439 ID=Msa0481110-mRNA-1:five_prime_utr;Parent=Msa0481110-mRNA-1
chr3_3 CDS 86862485 86862729 86862485 ID=Msa0481110-mRNA-1:cds;Parent=Msa0481110-mRNA-1
chr3_3 CDS 86862956 86863052 86862956 ID=Msa0481110-mRNA-1:cds;Parent=Msa0481110-mRNA-1
chr3_3 three_prime_UTR 86863053 86863121 86863053 ID=Msa0481110-mRNA-1:three_prime_utr;Parent=Msa0481110-mRNA-1
Gene Sequence

>Msa0481110

ATGGTTGGTCTTGGTGTTGTGCTTGAAGAACAACAACCACATAAGAAAAAGAGTAATATCAGCATCAACAACAACAATAACACTTGTCAAGTTATTAACAAAACTACCATGATGCTTAGCACAATCAACAACAAAACTTCTTCTCCACTTTCCTATCATTCTCCTTTTAAGGTTTCAACTTTTCTTGACCAATGCTTTCTATGCAGCAAGAAGCTCTTACCTGGGAAAGATATCTACATGTACAAAGGAGACAGAGCCTTTTGTAGCGTGGATTGTAGGTGCAAGCATATTTTAGCGGACGAGGAAGAGGCTATGAAGAAACAGAACATTTTTGAAGAGTAG

Protein sequence

>Msa0481110

MVGLGVVLEEQQPHKKKSNISINNNNNTCQVINKTTMMLSTINNKTSSPLSYHSPFKVSTFLDQCFLCSKKLLPGKDIYMYKGDRAFCSVDCRCKHILADEEEAMKKQNIFEE*