Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0100750 | XP_039684567.1 | 96.560 | 436 | 15 | 0 | 1 | 436 | 1 | 436 | 0.0 | 852 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0100750 | sp|P0DO21|GIP2_NICBE | 55.604 | 455 | 163 | 11 | 1 | 434 | 1 | 437 | 2.85e-158 | 457 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0052780 | Msa0100750 | 0.828016 | 1.136001e-54 | -8.615850e-47 |
| Msa0076640 | Msa0100750 | 0.825576 | 4.347926e-54 | -8.615850e-47 |
| Msa0077370 | Msa0100750 | 0.832328 | 1.006731e-55 | -8.615850e-47 |
| Msa0097570 | Msa0100750 | 0.827212 | 1.772489e-54 | -8.615850e-47 |
| Msa0100750 | Msa0125320 | 0.809385 | 1.942676e-50 | -8.615850e-47 |
| Msa0100750 | Msa0129640 | 0.817678 | 2.911989e-52 | -8.615850e-47 |
| Msa0100750 | Msa0132430 | 0.823581 | 1.282476e-53 | -8.615850e-47 |
| Msa0100750 | Msa0145430 | 0.842515 | 2.467165e-58 | -8.615850e-47 |
| Msa0100750 | Msa0182020 | 0.841725 | 3.992241e-58 | -8.615850e-47 |
| Msa0100750 | Msa0224210 | 0.857855 | 1.228148e-62 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0100750 | MtrunA17_Chr1g0187841 | 96.560 | 436 | 15 | 0 | 1 | 436 | 1 | 436 | 0.0 | 852 |
| Msa0100750 | MtrunA17_Chr1g0185661 | 53.556 | 450 | 173 | 8 | 3 | 435 | 655 | 1085 | 2.35e-148 | 449 |
| Msa0100750 | MtrunA17_Chr1g0185661 | 50.452 | 442 | 181 | 10 | 8 | 432 | 9 | 429 | 5.27e-132 | 406 |
| Msa0100750 | MtrunA17_Chr2g0287821 | 51.591 | 440 | 178 | 9 | 13 | 435 | 36 | 457 | 2.47e-143 | 417 |
| Msa0100750 | MtrunA17_Chr1g0185651 | 50.905 | 442 | 173 | 9 | 20 | 435 | 27 | 450 | 3.06e-141 | 411 |
| Msa0100750 | MtrunA17_Chr4g0065581 | 44.394 | 437 | 191 | 14 | 28 | 430 | 33 | 451 | 1.97e-100 | 307 |
| Msa0100750 | MtrunA17_Chr2g0287831 | 38.717 | 421 | 206 | 11 | 31 | 434 | 26 | 411 | 1.51e-79 | 252 |
| Msa0100750 | MtrunA17_Chr2g0287811 | 38.462 | 455 | 218 | 16 | 1 | 428 | 1 | 420 | 2.30e-74 | 239 |
| Msa0100750 | MtrunA17_Chr7g0273041 | 33.898 | 472 | 239 | 17 | 1 | 435 | 1 | 436 | 5.81e-70 | 228 |
| Msa0100750 | MtrunA17_Chr2g0287781 | 39.254 | 456 | 214 | 14 | 1 | 432 | 1 | 417 | 3.82e-67 | 232 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0100750 | AT1G03220.1 | 51.327 | 452 | 183 | 10 | 1 | 435 | 1 | 432 | 4.25e-142 | 413 |
| Msa0100750 | AT1G03230.1 | 50.465 | 430 | 177 | 9 | 23 | 435 | 23 | 433 | 4.09e-136 | 398 |
| Msa0100750 | AT5G19120.1 | 34.411 | 433 | 210 | 13 | 1 | 417 | 1 | 375 | 1.55e-55 | 189 |
| Msa0100750 | AT5G19110.1 | 31.944 | 432 | 245 | 13 | 10 | 430 | 8 | 401 | 1.68e-52 | 181 |
| Msa0100750 | AT5G19110.2 | 31.944 | 432 | 245 | 13 | 10 | 430 | 44 | 437 | 3.10e-52 | 181 |
| Msa0100750 | AT5G48430.1 | 28.982 | 452 | 251 | 18 | 1 | 431 | 1 | 403 | 1.03e-41 | 152 |
| Msa0100750 | AT5G19100.1 | 32.869 | 359 | 177 | 13 | 64 | 410 | 59 | 365 | 1.26e-41 | 152 |
| Msa0100750 | AT3G52500.1 | 24.628 | 471 | 246 | 23 | 12 | 417 | 36 | 462 | 1.26e-16 | 82.4 |
| Msa0100750 | AT3G25700.1 | 23.786 | 412 | 246 | 17 | 35 | 417 | 72 | 444 | 9.24e-16 | 79.3 |
| Msa0100750 | AT3G54400.1 | 23.333 | 390 | 228 | 13 | 44 | 417 | 85 | 419 | 6.99e-15 | 76.6 |
Find 78 sgRNAs with CRISPR-Local
Find 192 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCTGATGAAAATTGTGA+AGG | 0.597985 | 1_3:-7174145 | None:intergenic |
| AACCTAACTATTGATCTTGG+TGG | 0.524197 | 1_3:+7173788 | Msa0100750:CDS |
| AACTTGGAGCAGTGCTAACA+GGG | 0.559183 | 1_3:-7174294 | None:intergenic |
| AAGGATGAAGAGTTTAACTG+AGG | 0.626120 | 1_3:-7173615 | None:intergenic |
| AAGGGTGTCAAAGGTATGGC+TGG | 0.510377 | 1_3:+7174097 | Msa0100750:CDS |
| AAGGTATGGCTGGTCTTGGA+AGG | 0.572528 | 1_3:+7174107 | Msa0100750:CDS |
| AAGTATTAGTTCTTCAAGAA+TGG | 0.395644 | 1_3:+7174573 | Msa0100750:CDS |
| AATAAATTTGCAATTTGCTT+AGG | 0.381773 | 1_3:+7174178 | Msa0100750:CDS |
| AATGGTTCGTATTAATGATA+AGG | 0.488541 | 1_3:+7174669 | Msa0100750:CDS |
| AATGTGGTTTGGAATATTAT+TGG | 0.355763 | 1_3:+7174637 | Msa0100750:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCAAAGAGAGAAATGGTAAA+AGG | - | chr1_3:7173637-7173656 | None:intergenic | 30.0% | |
| ATCAACCTAACTATTGATCT+TGG | + | chr1_3:7173785-7173804 | Msa0100750:CDS | 30.0% | |
| TGCAGTGACAAAAAAATATG+TGG | + | chr1_3:7173911-7173930 | Msa0100750:CDS | 30.0% | |
| GGTTCAAATGTTGTACAAAA+TGG | + | chr1_3:7174067-7174086 | Msa0100750:CDS | 30.0% | |
| AAAGCTGATGAAAATTGTGA+AGG | - | chr1_3:7174148-7174167 | None:intergenic | 30.0% | |
| TCATCAGTGTTCAATAGATA+AGG | - | chr1_3:7174238-7174257 | None:intergenic | 30.0% | |
| GTGAAATCTATTAGAGTTTC+TGG | + | chr1_3:7174355-7174374 | Msa0100750:CDS | 30.0% | |
| CGTTAATCCTTACACAATAA+TGG | + | chr1_3:7174450-7174469 | Msa0100750:CDS | 30.0% | |
| TATTATTGGAGCTAATGCAA+TGG | + | chr1_3:7174651-7174670 | Msa0100750:CDS | 30.0% | |
| TTTGCAAAAACTTCTCAAGT+TGG | + | chr1_3:7174730-7174749 | Msa0100750:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 7173608 | 7174918 | 7173608 | ID=Msa0100750;Name=Msa0100750 |
| chr1_3 | mRNA | 7173608 | 7174918 | 7173608 | ID=Msa0100750-mRNA-1;Parent=Msa0100750;Name=Msa0100750-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|436 |
| chr1_3 | exon | 7173608 | 7174918 | 7173608 | ID=Msa0100750-mRNA-1:exon:2759;Parent=Msa0100750-mRNA-1 |
| chr1_3 | CDS | 7173608 | 7174918 | 7173608 | ID=Msa0100750-mRNA-1:cds;Parent=Msa0100750-mRNA-1 |
| Gene Sequence |
>Msa0100750 ATGGCTTCCTCAGTTAAACTCTTCATCCTTTTACCATTTCTCTCTTTGATCATCTCCACCTCCTTAGCACAAACCTCTTTCAGACCAAAAGCACTAGTTCTTCCCATAACCAGAGACACTTCAGCCTCAACACCTCAATACACTACTCAAATCAAACAAAGAACACCACTTGTACCAATCAACCTAACTATTGATCTTGGTGGTGGCTATTTTTGGGTGAACTGTGACAAGAGCTATGTCTCATCCACTTTAAAACCAATCCTATGTAGCTCATCTCAATGCTCACTCTTCGGTTCACATGGTTGCAGTGACAAAAAAATATGTGGCCGTTCACCTTACAACACTGTAACAGGTGTGTCAACATCTGGTGACATTCAGTCAGATGTTGTTTCAGTTCAATCCACAAATGGAAACTATTCTGGTAGATTTGTTTCTGTTCCTAACTTTCTCTTCATTTGTGGTTCAAATGTTGTACAAAATGGTCTTGCTAAGGGTGTCAAAGGTATGGCTGGTCTTGGAAGGACTAAAGTGTCACTTCCTTCACAATTTTCATCAGCTTTTAGTTTCAAGAATAAATTTGCAATTTGCTTAGGTACTCAAGATGGTGTACTTTTCTTTGGTGATGGACCTTATCTATTGAACACTGATGAATCTAAAAATCTTATTTTCACACCTCTTTTAACAAACCCTGTTAGCACTGCTCCAAGTTCTTTCTTAGGTGAAAAATCAGAAGAGTATTTTATTGGAGTGAAATCTATTAGAGTTTCTGGTAAAAATGTAAAGTTGAACACAACCCTTTTATCTATAGACCAAAATGGATTTGGTGGAACCAAAATTAGCACCGTTAATCCTTACACAATAATGGAGTCTTCAATTTATAAAGCAGTTGCTGATGCTTTTGTGAAAGCATTAAATGTTTCTACTGTTGAACCTGTAGCACCATTTGGAACATGTTTTGCAAGTCAAAGTATTAGTTCTTCAAGAATGGGACCAGATGTTCCTTCCATTGATCTTGTGTTGCAGAATGAGAATGTGGTTTGGAATATTATTGGAGCTAATGCAATGGTTCGTATTAATGATAAGGATGTGATTTGTTTGGGTTTTGTGGATGCTGGTTCTGATTTTGCAAAAACTTCTCAAGTTGGTTTTGTGGCTGGTGGTTCTAAACCAATGACTTCAATCATTATTGGTGCACATCAATTGGAGAATAATTTGTTGCAATTTGATTTGGCTACTTCAAGGCTTGGTTTTAGGTCACTCTTCTTGGAGCATGATAATTGTGCAAACTTTAATTTTACCTCATCTGTTTAG |
| Protein sequence |
>Msa0100750 MASSVKLFILLPFLSLIISTSLAQTSFRPKALVLPITRDTSASTPQYTTQIKQRTPLVPINLTIDLGGGYFWVNCDKSYVSSTLKPILCSSSQCSLFGSHGCSDKKICGRSPYNTVTGVSTSGDIQSDVVSVQSTNGNYSGRFVSVPNFLFICGSNVVQNGLAKGVKGMAGLGRTKVSLPSQFSSAFSFKNKFAICLGTQDGVLFFGDGPYLLNTDESKNLIFTPLLTNPVSTAPSSFLGEKSEEYFIGVKSIRVSGKNVKLNTTLLSIDQNGFGGTKISTVNPYTIMESSIYKAVADAFVKALNVSTVEPVAPFGTCFASQSISSSRMGPDVPSIDLVLQNENVVWNIIGANAMVRINDKDVICLGFVDAGSDFAKTSQVGFVAGGSKPMTSIIIGAHQLENNLLQFDLATSRLGFRSLFLEHDNCANFNFTSSV* |
