AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0636740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0636740 MtrunA17_Chr4g0033191 96.964 527 16 0 1 527 1 527 0.0 1069
Msa0636740 MtrunA17_Chr4g0033201 85.000 520 71 2 1 520 1 513 0.0 920
Msa0636740 MtrunA17_Chr4g0033271 76.008 521 115 3 1 520 1 512 0.0 826
Msa0636740 MtrunA17_Chr4g0033311 65.577 520 176 1 3 522 2 518 0.0 741
Msa0636740 MtrunA17_Chr3g0088441 64.217 517 183 1 6 522 3 517 0.0 729
Msa0636740 MtrunA17_Chr4g0033401 64.990 517 172 2 1 517 1 508 0.0 717
Msa0636740 MtrunA17_Chr6g0482131 64.147 516 179 2 7 521 4 514 0.0 717
Msa0636740 MtrunA17_Chr4g0033331 69.053 475 147 0 43 517 39 513 0.0 716
Msa0636740 MtrunA17_Chr2g0329201 64.648 512 174 2 8 519 3 507 0.0 716
Msa0636740 MtrunA17_Chr4g0033321 64.648 512 178 2 13 523 4 513 0.0 715
Msa0636740 MtrunA17_Chr6g0482071 64.272 515 180 1 5 519 3 513 0.0 707
Msa0636740 MtrunA17_Chr2g0329191 66.460 483 161 1 37 519 28 509 0.0 705
Msa0636740 MtrunA17_Chr6g0482081 66.472 513 168 3 8 518 2 512 0.0 700
Msa0636740 MtrunA17_Chr4g0033341 61.240 516 191 4 6 520 37 544 0.0 682
Msa0636740 MtrunA17_Chr2g0329321 61.868 514 163 2 6 519 8 488 0.0 666
Msa0636740 MtrunA17_Chr4g0033261 60.348 517 176 3 6 519 3 493 0.0 645
Msa0636740 MtrunA17_Chr4g0033231 59.127 504 175 3 18 519 19 493 0.0 632
Msa0636740 MtrunA17_Chr5g0430451 58.891 523 179 3 6 519 3 498 0.0 610
Msa0636740 MtrunA17_Chr4g0010501 58.491 477 192 3 43 519 24 494 0.0 581
Msa0636740 MtrunA17_Chr4g0006111 53.955 493 187 6 39 519 61 525 0.0 551
Msa0636740 MtrunA17_Chr3g0087261 51.373 510 239 6 11 519 8 509 0.0 547
Msa0636740 MtrunA17_Chr4g0006131 53.800 500 192 9 30 518 29 500 0.0 533
Msa0636740 MtrunA17_Chr4g0043091 53.512 484 212 5 41 518 26 502 0.0 526
Msa0636740 MtrunA17_Chr3g0087271 50.867 519 243 5 1 519 4 510 0.0 526
Msa0636740 MtrunA17_Chr6g0482941 48.438 512 252 8 9 519 12 512 3.48e-168 486
Msa0636740 MtrunA17_Chr6g0482951 48.242 512 253 8 9 519 12 512 9.67e-168 484
Msa0636740 MtrunA17_Chr4g0042171 49.385 488 239 5 43 526 24 507 1.57e-163 474
Msa0636740 MtrunA17_Chr4g0074561 49.260 473 235 5 48 517 40 510 7.10e-161 468
Msa0636740 MtrunA17_Chr2g0301231 55.370 419 150 5 111 519 1 392 1.94e-159 459
Msa0636740 MtrunA17_Chr2g0288071 47.490 518 251 7 8 523 48 546 5.57e-159 464
Msa0636740 MtrunA17_Chr4g0074551 48.536 478 240 6 48 523 55 528 1.29e-155 455
Msa0636740 MtrunA17_Chr3g0090131 46.507 501 243 7 27 524 11 489 3.99e-154 449
Msa0636740 MtrunA17_Chr3g0090111 46.901 484 239 6 43 524 19 486 6.72e-154 448
Msa0636740 MtrunA17_Chr2g0317821 45.090 499 259 7 33 523 18 509 3.98e-150 440
Msa0636740 MtrunA17_Chr2g0317841 44.489 499 262 7 33 523 18 509 4.78e-149 437
Msa0636740 MtrunA17_Chr2g0288021 42.607 514 277 7 8 519 12 509 2.62e-147 432
Msa0636740 MtrunA17_Chr7g0242691 45.155 485 238 7 43 519 31 495 3.04e-146 430
Msa0636740 MtrunA17_Chr7g0242681 46.281 484 231 9 43 518 38 500 9.50e-144 424
Msa0636740 MtrunA17_Chr4g0006971 44.008 484 253 7 45 525 29 497 2.38e-139 412
Msa0636740 MtrunA17_Chr5g0428831 44.491 481 246 8 44 520 28 491 4.59e-138 409
Msa0636740 MtrunA17_Chr1g0195611 43.892 483 249 7 42 520 26 490 2.12e-134 399
Msa0636740 MtrunA17_Chr3g0087201 53.754 333 149 2 187 519 17 344 5.83e-128 377
Msa0636740 MtrunA17_Chr7g0234091 44.835 484 241 8 44 523 76 537 1.46e-126 380
Msa0636740 MtrunA17_Chr7g0234111 45.168 476 239 6 44 517 76 531 4.84e-125 376
Msa0636740 MtrunA17_Chr7g0234131 43.277 476 249 5 44 517 76 532 1.23e-122 370
Msa0636740 MtrunA17_Chr5g0418871 55.667 300 130 2 61 360 1 297 5.33e-120 356
Msa0636740 MtrunA17_Chr1g0195641 46.770 387 191 6 45 428 29 403 2.71e-114 344
Msa0636740 MtrunA17_Chr7g0230651 44.267 375 193 5 90 464 18 376 2.89e-101 310
Msa0636740 MtrunA17_Chr3g0087191 69.375 160 42 1 34 186 6 165 1.84e-77 241
Msa0636740 MtrunA17_Chr7g0236731 48.085 235 115 2 132 365 28 256 1.06e-61 204
Msa0636740 MtrunA17_Chr7g0227561 36.656 311 157 6 9 313 7 283 4.03e-60 200
Msa0636740 MtrunA17_Chr4g0068871 27.098 417 231 12 107 517 183 532 2.08e-28 119
Msa0636740 MtrunA17_Chr5g0418861 58.427 89 36 1 430 517 2 90 1.07e-27 106
Msa0636740 MtrunA17_Chr8g0352881 45.000 100 55 0 269 368 57 156 1.18e-23 97.4
Msa0636740 MtrunA17_Chr8g0352881 48.571 70 35 1 52 121 7 75 1.17e-12 66.2
Msa0636740 MtrunA17_Chr7g0227591 54.386 57 26 0 465 521 13 69 1.74e-14 68.6
Msa0636740 MtrunA17_Chr2g0288031 46.552 58 30 1 195 252 4 60 1.18e-11 60.1
Msa0636740 MtrunA17_Chr2g0301221 72.222 36 10 0 48 83 70 105 4.37e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0636740 AT5G44640.1 51.859 511 230 4 10 519 8 503 0.0 565
Msa0636740 AT5G42260.1 52.756 508 226 5 7 513 3 497 0.0 561
Msa0636740 AT2G44480.3 53.144 493 227 2 28 520 54 542 0.0 561
Msa0636740 AT2G44480.1 53.144 493 227 2 28 520 23 511 0.0 560
Msa0636740 AT2G44450.1 54.184 478 212 3 43 519 31 502 0.0 560
Msa0636740 AT3G60130.1 54.507 477 211 3 43 518 30 501 0.0 541
Msa0636740 AT2G25630.1 51.176 510 217 6 9 517 5 483 0.0 530
Msa0636740 AT5G24550.1 50.688 509 245 6 17 520 5 512 0.0 525
Msa0636740 AT1G26560.1 50.413 484 232 6 41 520 30 509 1.89e-175 504
Msa0636740 AT5G24540.1 50.884 509 244 6 17 520 5 512 2.05e-175 505
Msa0636740 AT3G60140.1 47.868 516 249 6 9 521 8 506 3.50e-175 506
Msa0636740 AT2G44460.1 49.614 518 247 7 10 523 3 510 1.29e-171 497
Msa0636740 AT2G44470.3 47.002 517 255 6 10 519 3 507 4.26e-171 496
Msa0636740 AT5G54570.1 51.566 479 225 5 43 517 30 505 1.09e-170 493
Msa0636740 AT1G51470.1 49.692 487 231 6 35 519 37 511 3.77e-170 491
Msa0636740 AT1G47600.1 49.491 491 226 7 35 519 37 511 7.05e-169 488
Msa0636740 AT1G47600.2 49.287 491 226 7 35 519 37 510 6.84e-168 485
Msa0636740 AT3G60120.1 50.309 485 230 7 41 519 12 491 1.13e-167 486
Msa0636740 AT3G18080.1 49.583 480 232 7 43 520 39 510 4.54e-165 478
Msa0636740 AT2G44490.1 49.269 479 237 5 45 518 16 493 2.95e-164 478
Msa0636740 AT2G44480.2 52.195 410 195 1 111 520 1 409 6.40e-163 469
Msa0636740 AT5G25980.2 46.360 522 260 11 12 524 19 529 1.54e-158 462
Msa0636740 AT3G60130.2 53.012 415 189 3 105 518 40 449 1.87e-158 459
Msa0636740 AT3G18070.3 46.875 480 235 9 43 519 30 492 1.29e-157 458
Msa0636740 AT5G36890.1 48.682 493 240 4 33 524 6 486 5.66e-157 457
Msa0636740 AT2G32860.2 46.107 488 259 4 42 525 93 580 7.17e-157 461
Msa0636740 AT5G36890.2 48.682 493 240 4 33 524 6 486 8.81e-157 456
Msa0636740 AT3G18070.1 46.875 480 241 9 43 519 30 498 3.62e-156 455
Msa0636740 AT3G03640.1 44.874 517 271 6 8 519 7 514 1.54e-155 454
Msa0636740 AT2G32860.1 46.107 488 258 5 42 525 93 579 1.70e-155 457
Msa0636740 AT5G26000.1 46.080 523 255 10 8 517 3 511 9.73e-155 453
Msa0636740 AT1G61820.1 48.276 493 246 7 32 521 22 508 6.56e-154 450
Msa0636740 AT1G66280.1 44.592 527 275 8 1 522 1 515 1.14e-149 439
Msa0636740 AT3G60130.3 51.566 415 184 4 105 518 40 438 1.85e-149 436
Msa0636740 AT3G09260.1 45.963 483 256 5 43 522 35 515 2.04e-149 439
Msa0636740 AT1G02850.2 46.804 485 231 8 44 522 27 490 8.00e-149 436
Msa0636740 AT2G44470.1 46.567 466 227 6 4 463 1 450 2.00e-148 433
Msa0636740 AT5G24540.2 49.446 451 223 5 17 463 5 454 3.85e-148 433
Msa0636740 AT1G75940.1 46.170 483 250 7 43 517 37 517 4.69e-148 436
Msa0636740 AT5G54570.3 51.294 425 201 4 43 464 30 451 1.05e-147 431
Msa0636740 AT1G02850.5 45.059 506 228 9 44 520 27 511 4.13e-144 425
Msa0636740 AT5G28510.1 43.992 491 259 7 43 522 39 524 9.06e-144 424
Msa0636740 AT3G21370.1 44.969 487 250 9 43 518 33 512 1.42e-143 424
Msa0636740 AT3G60140.2 47.418 426 205 5 9 432 8 416 3.32e-143 420
Msa0636740 AT1G52400.3 44.146 521 275 11 6 518 4 516 1.59e-142 421
Msa0636740 AT1G52400.1 44.146 521 275 11 6 518 4 516 1.59e-142 421
Msa0636740 AT1G66270.1 44.856 486 260 6 41 522 34 515 1.83e-142 421
Msa0636740 AT1G02850.4 44.970 507 228 10 44 520 27 512 2.07e-142 421
Msa0636740 AT4G21760.2 44.280 472 252 6 48 517 59 521 3.28e-142 421
Msa0636740 AT1G61810.1 42.278 518 289 6 10 524 4 514 3.71e-142 420
Msa0636740 AT4G27830.1 42.276 492 263 7 34 521 14 488 1.35e-140 416
Msa0636740 AT5G54570.2 50.122 411 198 4 111 517 1 408 1.49e-140 413
Msa0636740 AT5G25980.3 46.522 460 228 9 12 464 19 467 2.69e-139 410
Msa0636740 AT5G25980.1 46.522 460 228 9 12 464 19 467 2.69e-139 410
Msa0636740 AT1G66270.2 44.650 486 259 7 41 522 34 513 2.76e-139 412
Msa0636740 AT1G02850.1 45.567 485 210 9 44 522 27 463 4.39e-139 410
Msa0636740 AT2G44480.4 57.055 326 135 3 28 353 54 374 9.15e-137 401
Msa0636740 AT4G22100.1 43.867 481 244 9 44 518 23 483 6.79e-136 403
Msa0636740 AT1G60090.2 43.035 481 248 9 43 518 24 483 1.00e-135 403
Msa0636740 AT4G21760.1 43.644 472 245 7 48 517 59 511 1.24e-135 404
Msa0636740 AT5G26000.2 44.776 469 236 7 6 464 1 456 1.67e-135 400
Msa0636740 AT1G61810.3 42.570 498 276 6 10 504 4 494 3.22e-135 403
Msa0636740 AT2G44450.2 52.101 357 164 3 164 519 1 351 1.10e-133 392
Msa0636740 AT1G60090.1 42.355 484 254 10 43 518 24 490 3.39e-132 394
Msa0636740 AT4G27820.1 42.236 483 256 7 43 521 23 486 2.34e-131 392
Msa0636740 AT1G45191.4 42.292 480 254 9 41 518 26 484 3.87e-129 386
Msa0636740 AT2G44470.2 47.906 382 185 4 12 387 5 378 4.74e-129 382
Msa0636740 AT1G45191.2 41.822 483 258 9 41 518 26 490 1.03e-126 380
Msa0636740 AT1G45191.6 41.309 489 257 9 41 518 26 495 5.11e-126 379
Msa0636740 AT3G62750.1 40.039 517 260 12 5 517 3 473 1.09e-125 377
Msa0636740 AT3G62750.2 39.731 521 264 12 5 521 29 503 2.79e-125 377
Msa0636740 AT1G45191.1 41.176 493 264 9 41 518 26 507 3.52e-123 372
Msa0636740 AT1G52400.2 43.441 465 248 10 6 463 4 460 4.10e-119 359
Msa0636740 AT1G02850.3 45.278 413 183 6 44 428 27 424 2.25e-118 356
Msa0636740 AT3G62740.1 37.079 534 280 12 9 526 4 497 3.75e-118 358
Msa0636740 AT5G48375.1 41.372 452 204 7 61 510 47 439 1.14e-116 352
Msa0636740 AT1G61810.5 42.754 414 231 5 114 524 4 414 1.59e-114 345
Msa0636740 AT2G44460.2 49.180 366 180 5 162 523 1 364 4.57e-113 342
Msa0636740 AT4G27830.2 41.205 415 225 6 111 521 1 400 4.98e-112 339
Msa0636740 AT1G61810.4 41.232 422 239 5 10 428 4 419 1.23e-110 336
Msa0636740 AT3G18070.4 49.062 320 157 4 43 362 30 343 1.17e-109 332
Msa0636740 AT1G60270.1 49.684 316 151 4 41 355 21 329 2.09e-108 328
Msa0636740 AT3G62750.4 40.215 465 229 11 5 466 29 447 1.25e-107 329
Msa0636740 AT1G61820.3 45.380 368 196 4 157 521 4 369 9.01e-105 319
Msa0636740 AT4G27820.2 40.723 415 228 6 111 521 1 401 2.12e-103 317
Msa0636740 AT4G27820.3 40.723 415 228 6 111 521 1 401 2.12e-103 317
Msa0636740 AT1G51490.1 46.910 356 164 8 46 391 17 357 2.74e-103 317
Msa0636740 AT3G62740.2 37.609 460 242 10 9 463 4 423 2.16e-102 315
Msa0636740 AT2G44480.5 48.173 301 155 1 220 520 6 305 2.46e-101 308
Msa0636740 AT1G45191.3 45.820 323 166 4 41 362 26 340 9.98e-100 307
Msa0636740 AT1G45191.5 45.820 323 166 4 41 362 26 340 1.03e-99 307
Msa0636740 AT3G62750.7 42.382 361 172 8 5 364 3 328 1.13e-99 306
Msa0636740 AT3G62750.8 42.382 361 172 8 5 364 3 328 1.13e-99 306
Msa0636740 AT3G62750.3 43.213 361 169 8 5 364 29 354 8.74e-99 306
Msa0636740 AT3G18070.2 45.732 328 169 6 195 519 100 421 3.94e-98 303
Msa0636740 AT3G18070.2 53.968 63 28 1 43 105 30 91 4.36e-14 74.7
Msa0636740 AT3G62750.6 39.518 415 224 8 111 521 1 392 4.43e-98 303
Msa0636740 AT3G62750.5 39.518 415 224 8 111 521 1 392 4.43e-98 303
Msa0636740 AT3G18070.7 45.397 315 163 6 208 519 1 309 1.76e-92 285
Msa0636740 AT1G61810.2 45.215 303 160 3 10 312 4 300 6.13e-89 276
Msa0636740 AT1G60270.2 49.383 243 120 2 113 355 3 242 2.61e-80 253
Msa0636740 AT3G18070.5 43.644 236 129 3 195 428 100 333 3.86e-65 215
Msa0636740 AT3G18070.5 53.968 63 28 1 43 105 30 91 3.19e-14 74.7
Msa0636740 AT3G18070.6 43.049 223 123 3 208 428 1 221 8.95e-60 197
Msa0636740 AT5G16580.1 37.631 287 164 7 189 474 27 299 6.69e-51 176
Msa0636740 AT3G06510.1 25.537 419 239 13 106 518 159 510 1.26e-29 123
Msa0636740 AT3G06510.2 25.342 438 250 13 106 518 159 544 3.00e-29 122

Find 140 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAGCTTCTTCTGCATTTC+AGG 0.143961 4_3:+27643600 Msa0636740:CDS
ATGGAAAAGGACCAAGTATT+TGG 0.197865 4_3:+27643783 Msa0636740:CDS
GACCTTTGCTACAAGGAATT+TGG 0.209271 4_3:+27645074 Msa0636740:CDS
GCATCTCAATATGGTATAAT+AGG 0.209400 4_3:+27645469 Msa0636740:CDS
GTTCTATGGTTTCCCTTTAT+AGG 0.230542 4_3:-27643369 None:intergenic
CGATTCTATAAGTATCTAAA+AGG 0.249841 4_3:-27646640 None:intergenic
ATAATATACATTACTGAAAA+TGG 0.254622 4_3:+27646477 Msa0636740:CDS
CCTCCCTCCTCCTCCTATAA+AGG 0.257504 4_3:+27643356 Msa0636740:five_prime_UTR
GTGCATAAACATCATTAATA+TGG 0.263362 4_3:+27643417 Msa0636740:exon
AAAACAGCAGACCTGATATT+TGG 0.263998 4_3:-27645297 None:intergenic
AAATTGAAGGTAACGTTCTA+TGG 0.276797 4_3:-27643383 None:intergenic
AATTATGGATTCGATTATTT+AGG 0.280439 4_3:-27643280 None:intergenic
AATCATAAAGGATTTGAATA+TGG 0.307459 4_3:+27644549 Msa0636740:CDS
TATAGCTATTCTCCATAAAA+AGG 0.308127 4_3:-27647304 None:intergenic
AAGATCCTCGGATTCTTCTT+TGG 0.311879 4_3:-27645964 None:intergenic
AACAATGGTGGTGGCGATAA+TGG 0.312671 4_3:-27643482 None:intergenic
TAAATAATAAAATAAAATTA+TGG 0.317471 4_3:-27643295 None:intergenic
AATTGCACAGGAGGTGATTC+GGG 0.327589 4_3:+27645206 Msa0636740:CDS
AACCAATGTGCCGAGAGTTT+TGG 0.337227 4_3:-27646964 None:intergenic
TGCATTTATCCGAGAGGATT+AGG 0.340389 4_3:+27646414 Msa0636740:CDS
CGAGGCCTCGACTTCATGTT+TGG 0.348528 4_3:+27645559 Msa0636740:CDS
AGAAGAATCCGAGGATCTTA+AGG 0.358352 4_3:+27645968 Msa0636740:CDS
GATTCTTCGAGTGACAGTTT+TGG 0.360269 4_3:-27646617 None:intergenic
GGACGTTCCTCAAGCCTTAG+AGG 0.360927 4_3:+27644887 Msa0636740:CDS
TCTGGCTTCAGCTTGCGATT+TGG 0.362792 4_3:+27646901 Msa0636740:CDS
TATGTATTTCAGGAAGATAT+TGG 0.369128 4_3:+27644526 Msa0636740:intron
GCTACAAGGAATTTGGTGAT+AGG 0.371977 4_3:+27645081 Msa0636740:CDS
AACAAAGCCTGGCGGGAAAC+AGG 0.373667 4_3:-27643572 None:intergenic
GATAAGAGAGTCAGTTTGTA+TGG 0.374381 4_3:-27646072 None:intergenic
TGAAGCCAGATTCCCATTCA+TGG 0.380755 4_3:-27646888 None:intergenic
CACCGGGTCGATGTTCTGAT+TGG 0.383994 4_3:+27645174 Msa0636740:CDS
AAAATAAGAGATGGAGGTAA+TGG 0.386283 4_3:+27644401 Msa0636740:CDS
GGAAGATATTGGAATCATAA+AGG 0.386661 4_3:+27644537 Msa0636740:intron
AGGATTAGAAAATAGTGATA+TGG 0.390437 4_3:+27647041 Msa0636740:three_prime_UTR
GAATTGCACAGGAGGTGATT+CGG 0.391598 4_3:+27645205 Msa0636740:CDS
GTCAGTTTGTATGGCTGGTC+TGG 0.402360 4_3:-27646063 None:intergenic
TGGAAAAGGACCAAGTATTT+GGG 0.404129 4_3:+27643784 Msa0636740:CDS
CAACCTCATCAACGAACTAT+TGG 0.405447 4_3:+27644746 Msa0636740:CDS
GATAACCATGAATGGGAATC+TGG 0.405714 4_3:+27646883 Msa0636740:CDS
CATCTCAATATGGTATAATA+GGG 0.411825 4_3:+27645470 Msa0636740:CDS
AGGTGCATCAGCAGCATAAG+AGG 0.414938 4_3:-27646027 None:intergenic
TGTAGTAGTTTATTCCTTCA+CGG 0.418155 4_3:-27644722 None:intergenic
CTCCCTCCTCCTCCTATAAA+GGG 0.425999 4_3:+27643357 Msa0636740:five_prime_UTR
TATTCTTGACGTAAAGCAAC+AGG 0.430967 4_3:-27646440 None:intergenic
AAGAGGAAGAGTAATAGTTT+AGG 0.433722 4_3:-27646010 None:intergenic
TGTTCTGTTTAAATGAACTT+AGG 0.434940 4_3:+27647021 Msa0636740:exon
AGGGAAGGTGTGAATGTAAA+GGG 0.435489 4_3:+27646838 Msa0636740:intron
GCGATTTGGACTCGTCTTTG+TGG 0.435971 4_3:+27646915 Msa0636740:CDS
GAAATGCAGGCATCTCAATA+TGG 0.438902 4_3:+27645460 Msa0636740:intron
ATTATGTTCAAGCTGCAATC+AGG 0.440754 4_3:+27646684 Msa0636740:CDS
GATCGGAAAAGTCGCGAAGA+TGG 0.446253 4_3:-27643540 None:intergenic
TAATGATGTTTATGCACAAA+GGG 0.446540 4_3:-27643411 None:intergenic
TTTGCAGAAGGAAAGCTTAG+TGG 0.446993 4_3:+27644690 Msa0636740:intron
ACTATACAAGACCAAATATC+AGG 0.449280 4_3:+27645286 Msa0636740:CDS
ATCAACGAACTATTGGCTAA+TGG 0.455297 4_3:+27644753 Msa0636740:CDS
GGATTACCTTACTCTCTCAT+TGG 0.457583 4_3:+27645491 Msa0636740:CDS
CTTTGGAGAACTTTGGTAGC+CGG 0.461441 4_3:-27645947 None:intergenic
GAAGAATCCGAGGATCTTAA+GGG 0.463005 4_3:+27645969 Msa0636740:CDS
AGAGAGTCAGTTTGTATGGC+TGG 0.463847 4_3:-27646068 None:intergenic
AATCGGACCTGTTTCCCGCC+AGG 0.464039 4_3:+27643565 Msa0636740:CDS
CTACAAGGAATTTGGTGATA+GGG 0.466831 4_3:+27645082 Msa0636740:CDS
ACCGTCATACTCATCCTCTA+AGG 0.470920 4_3:-27644901 None:intergenic
GATTCTTCTTTGGAGAACTT+TGG 0.475194 4_3:-27645954 None:intergenic
TGGTTTCCCTTTATAGGAGG+AGG 0.483745 4_3:-27643363 None:intergenic
TGCGATCTTTGGTTGGAAGC+CGG 0.484554 4_3:+27645928 Msa0636740:CDS
AGCATGAATGCTTATGCATA+TGG 0.485187 4_3:+27645143 Msa0636740:CDS
CATCGTGATGGCAAGCCCCT+TGG 0.493086 4_3:+27646243 Msa0636740:CDS
TTCCGAAAACAAAGCCTGGC+GGG 0.497302 4_3:-27643579 None:intergenic
CAGGGAAGGTGTGAATGTAA+AGG 0.498317 4_3:+27646837 Msa0636740:intron
CTCATACCAATGAGAGAGTA+AGG 0.499326 4_3:-27645497 None:intergenic
CAGCAGCATGAGCAAGAAGT+TGG 0.509755 4_3:-27645256 None:intergenic
TATATGGATCCAATCACAAA+AGG 0.510867 4_3:+27645888 Msa0636740:CDS
TGGTAGTGCGCAGTCAAATA+CGG 0.513115 4_3:-27645236 None:intergenic
CTACGCTGACCTTTGCTACA+AGG 0.513290 4_3:+27645067 Msa0636740:CDS
CATTGTTGGATAACCATGAA+TGG 0.514197 4_3:+27646875 Msa0636740:CDS
CACCAAATTCCTTGTAGCAA+AGG 0.520911 4_3:-27645076 None:intergenic
ATTGTTGGATAACCATGAAT+GGG 0.522990 4_3:+27646876 Msa0636740:CDS
ACAAAGGGAAATGAAATTGA+AGG 0.523224 4_3:-27643396 None:intergenic
TCTGCTTTCGCTTGAGAGGC+CGG 0.524404 4_3:-27645520 None:intergenic
AACATCTGCTTTCGCTTGAG+AGG 0.524538 4_3:-27645524 None:intergenic
CTATGGTTTCCCTTTATAGG+AGG 0.527230 4_3:-27643366 None:intergenic
TGGTTAAATCTGAATTGCAC+AGG 0.528273 4_3:+27645194 Msa0636740:CDS
AACAGGTTTCCTAATCCTCT+CGG 0.530574 4_3:-27646423 None:intergenic
TTGGTGATAGGGTGAAACAT+TGG 0.532663 4_3:+27645093 Msa0636740:CDS
ACTCTCTCATTGGTATGAGC+CGG 0.532738 4_3:+27645501 Msa0636740:CDS
TGGCAAGCCCCTTGGTCCAA+TGG 0.533454 4_3:+27646251 Msa0636740:CDS
TGCATATGGAAAGTTTGCAC+CGG 0.533544 4_3:+27645157 Msa0636740:CDS
GCAGCATTTGAAGATGGAAA+AGG 0.538475 4_3:+27643770 Msa0636740:CDS
GCTGTTCCGAAAACAAAGCC+TGG 0.539523 4_3:-27643583 None:intergenic
GATTCTATAAGTATCTAAAA+GGG 0.541771 4_3:-27646639 None:intergenic
AATATATACCATTGGACCAA+GGG 0.544956 4_3:-27646259 None:intergenic
TTTCCCTTTATAGGAGGAGG+AGG 0.546300 4_3:-27643360 None:intergenic
AAAAGCATGCGATCTTTGGT+TGG 0.548161 4_3:+27645921 Msa0636740:CDS
TCGATGACAGTTCATCGTGA+TGG 0.548253 4_3:+27646231 Msa0636740:intron
GCCTCGACTTCATGTTTGGA+TGG 0.549073 4_3:+27645563 Msa0636740:CDS
GAAGGTGCAGCATTTGAAGA+TGG 0.552430 4_3:+27643764 Msa0636740:CDS
GTGAAAGTATCCCAAATACT+TGG 0.559968 4_3:-27643794 None:intergenic
TAGCCAATAGTTCGTTGATG+AGG 0.565702 4_3:-27644749 None:intergenic
TTTCAGTAATGTATATTATG+GGG 0.565878 4_3:-27646473 None:intergenic
ATATGCAGTCTGCTTCAAGT+TGG 0.568943 4_3:+27646388 Msa0636740:intron
CGAAGATGGAGTAGTGAAGA+AGG 0.570282 4_3:-27643526 None:intergenic
CGACGCATATCATCGGTACA+AGG 0.572001 4_3:+27644436 Msa0636740:CDS
GCATTCATGCTCACACTCCA+TGG 0.576199 4_3:-27645131 None:intergenic
GAAACAGGTCCGATTGAGAT+CGG 0.577440 4_3:-27643557 None:intergenic
CAATGGAAAAGAGTCACATA+TGG 0.580739 4_3:-27644864 None:intergenic
AGCATTGATATGAGAAACAA+TGG 0.584246 4_3:-27643497 None:intergenic
GAAAAGACACCCAAAACTCT+CGG 0.586397 4_3:+27646954 Msa0636740:CDS
TTAATGATGTTTATGCACAA+AGG 0.589128 4_3:-27643412 None:intergenic
GCATATGGAAAGTTTGCACC+GGG 0.592559 4_3:+27645158 Msa0636740:CDS
TACTCATCCTCTAAGGCTTG+AGG 0.594134 4_3:-27644894 None:intergenic
CCTTTATAGGAGGAGGAGGG+AGG 0.597186 4_3:-27643356 None:intergenic
TAGCCGACGACGCATATCAT+CGG 0.598194 4_3:+27644429 Msa0636740:CDS
AAATATATACCATTGGACCA+AGG 0.598574 4_3:-27646260 None:intergenic
ACCCAAAACTCTCGGCACAT+TGG 0.602316 4_3:+27646962 Msa0636740:CDS
TTCCCTTTATAGGAGGAGGA+GGG 0.603110 4_3:-27643359 None:intergenic
GGATTACTTTGAATGAACCA+TGG 0.603853 4_3:+27645114 Msa0636740:CDS
AGGCTTGAGGAACGTCCCAA+TGG 0.604842 4_3:-27644881 None:intergenic
GTTTAGGCCAAGAAAATCGA+AGG 0.606609 4_3:-27645994 None:intergenic
GAAGGACCCCTTAAGATCCT+CGG 0.611639 4_3:-27645976 None:intergenic
GGTGTCACTCACACAATCCG+AGG 0.616673 4_3:-27644933 None:intergenic
GGTCTGGCATCGCGTAAGTG+AGG 0.617392 4_3:-27646047 None:intergenic
GCCTTAGAGGATGAGTATGA+CGG 0.628770 4_3:+27644900 Msa0636740:CDS
AACCAATCAGAACATCGACC+CGG 0.630892 4_3:-27645176 None:intergenic
GCAGAAGGAAAGCTTAGTGG+AGG 0.637778 4_3:+27644693 Msa0636740:intron
ATATATACCATTGGACCAAG+GGG 0.639799 4_3:-27646258 None:intergenic
AGTGGAGGTGTAAACCGTGA+AGG 0.641058 4_3:+27644708 Msa0636740:CDS
ACCATCCAAACATGAAGTCG+AGG 0.641696 4_3:-27645564 None:intergenic
GTTCTCCAAAGAAGAATCCG+AGG 0.647399 4_3:+27645959 Msa0636740:CDS
TGGTTATGCATTTATCCGAG+AGG 0.653174 4_3:+27646408 Msa0636740:CDS
TAGCACTCTAGACCAAGCAA+TGG 0.660595 4_3:-27644587 None:intergenic
GTGTCACTCACACAATCCGA+GGG 0.662250 4_3:-27644932 None:intergenic
TTAAATCTGAATTGCACAGG+AGG 0.663041 4_3:+27645197 Msa0636740:CDS
GATATGAGAAACAATGGTGG+TGG 0.665043 4_3:-27643491 None:intergenic
GTACCGATGATATGCGTCGT+CGG 0.669538 4_3:-27644432 None:intergenic
AAGAATCCGAGGATCTTAAG+GGG 0.674534 4_3:+27645970 Msa0636740:CDS
ATTGCACAGGAGGTGATTCG+GGG 0.685472 4_3:+27645207 Msa0636740:CDS
ACTTTCACTCATGAACACCC+AGG 0.690909 4_3:+27643809 Msa0636740:CDS
GTTCCGAAAACAAAGCCTGG+CGG 0.701608 4_3:-27643580 None:intergenic
GATGTTGATGCTGCACAACG+AGG 0.729333 4_3:+27645541 Msa0636740:CDS
ATTGATATGAGAAACAATGG+TGG 0.754854 4_3:-27643494 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAATAAAATAAAATTA+TGG - chr4_3:27643298-27643317 None:intergenic 0.0%
!!! TTAGATTTTTTTTTTATTCA+AGG + chr4_3:27643327-27643346 Msa0636740:five_prime_UTR 10.0%
!!! TTAAAAATTTTGTAGTATGA+AGG + chr4_3:27643746-27643765 Msa0636740:intron 15.0%
!! ATATTAATATCATGTTTCTT+TGG + chr4_3:27643872-27643891 Msa0636740:intron 15.0%
!! TCTCATATATAATAATGAAA+TGG + chr4_3:27644369-27644388 Msa0636740:intron 15.0%
!! AGCAATATATTATTCAAATT+CGG + chr4_3:27645324-27645343 Msa0636740:intron 15.0%
!! AATGATAAAATATATACCAT+TGG - chr4_3:27646270-27646289 None:intergenic 15.0%
!!! TTTTCAGTAATGTATATTAT+GGG - chr4_3:27646477-27646496 None:intergenic 15.0%
!!! ATTTTCAGTAATGTATATTA+TGG - chr4_3:27646478-27646497 None:intergenic 15.0%
!! ATAATATACATTACTGAAAA+TGG + chr4_3:27646477-27646496 Msa0636740:CDS 15.0%
!! AATAATAGGTAAATGTAAAA+AGG - chr4_3:27646802-27646821 None:intergenic 15.0%
!! AATTATGGATTCGATTATTT+AGG - chr4_3:27643283-27643302 None:intergenic 20.0%
!! GAAGTAATTAAAAATAACCT+GGG - chr4_3:27643829-27643848 None:intergenic 20.0%
!! AGAAGTAATTAAAAATAACC+TGG - chr4_3:27643830-27643849 None:intergenic 20.0%
!! TAAATCGTTAGATTAAGATA+GGG + chr4_3:27643975-27643994 Msa0636740:intron 20.0%
!! AATCATAAAGGATTTGAATA+TGG + chr4_3:27644549-27644568 Msa0636740:CDS 20.0%
!! TTACAAAAATATGACTTACT+TGG - chr4_3:27644613-27644632 None:intergenic 20.0%
!! ACTATATCACAAATGTATAA+GGG - chr4_3:27644644-27644663 None:intergenic 20.0%
!! TACTATATCACAAATGTATA+AGG - chr4_3:27644645-27644664 None:intergenic 20.0%
!! ATAATAATTGAGAATAATGC+AGG + chr4_3:27644834-27644853 Msa0636740:intron 20.0%
!! CTATTTATCTATGACTATTT+AGG + chr4_3:27644972-27644991 Msa0636740:intron 20.0%
!!! GCATTTCTCAAAATTTTATT+TGG - chr4_3:27645447-27645466 None:intergenic 20.0%
!!! AATAAAATTTTGAGAAATGC+AGG + chr4_3:27645447-27645466 Msa0636740:intron 20.0%
!!! ATGTAAACTTCATTTGATTT+TGG + chr4_3:27645713-27645732 Msa0636740:intron 20.0%
!! TTTCAGTAATGTATATTATG+GGG - chr4_3:27646476-27646495 None:intergenic 20.0%
!! GATTCTATAAGTATCTAAAA+GGG - chr4_3:27646642-27646661 None:intergenic 20.0%
!! GAAAAAAAATTGCCAATAAA+AGG + chr4_3:27647097-27647116 Msa0636740:three_prime_UTR 20.0%
! TAATGATGTTTATGCACAAA+GGG - chr4_3:27643414-27643433 None:intergenic 25.0%
! TTAATGATGTTTATGCACAA+AGG - chr4_3:27643415-27643434 None:intergenic 25.0%
! GTGCATAAACATCATTAATA+TGG + chr4_3:27643417-27643436 Msa0636740:exon 25.0%
!! ATCATGTTTCTTTGGTAATA+TGG + chr4_3:27643880-27643899 Msa0636740:intron 25.0%
!! TCATGTTTCTTTGGTAATAT+GGG + chr4_3:27643881-27643900 Msa0636740:intron 25.0%
! GTAAATCGTTAGATTAAGAT+AGG + chr4_3:27643974-27643993 Msa0636740:intron 25.0%
! AATTCATATCCGTTCATTTA+GGG + chr4_3:27644051-27644070 Msa0636740:intron 25.0%
! AAATGTTGCACTAAATTGAA+TGG - chr4_3:27644134-27644153 None:intergenic 25.0%
!! CTTTTCCTTAATGATGTATT+TGG + chr4_3:27644215-27644234 Msa0636740:intron 25.0%
! TATGTATTTCAGGAAGATAT+TGG + chr4_3:27644526-27644545 Msa0636740:intron 25.0%
!!! TACTTATTTTCATATCCATG+TGG + chr4_3:27644798-27644817 Msa0636740:intron 25.0%
! AATAGCTCAAGATTGAAAAT+TGG - chr4_3:27644957-27644976 None:intergenic 25.0%
!!! GCAATTGTTATTTGAATTTC+AGG - chr4_3:27645366-27645385 None:intergenic 25.0%
! CATCTCAATATGGTATAATA+GGG + chr4_3:27645470-27645489 Msa0636740:CDS 25.0%
! ATGATATGATCATGATAATC+TGG + chr4_3:27645680-27645699 Msa0636740:intron 25.0%
! ATTACTTTACTTTGCACAAA+TGG - chr4_3:27645806-27645825 None:intergenic 25.0%
! TTTGTGCAAAGTAAAGTAAT+AGG + chr4_3:27645806-27645825 Msa0636740:intron 25.0%
! CGATTCTATAAGTATCTAAA+AGG - chr4_3:27646643-27646662 None:intergenic 25.0%
! TAGGTAAATGTAAAAAGGAA+GGG - chr4_3:27646797-27646816 None:intergenic 25.0%
! ATAGGTAAATGTAAAAAGGA+AGG - chr4_3:27646798-27646817 None:intergenic 25.0%
! TATGCATAAAGGTCAATAAT+AGG - chr4_3:27646816-27646835 None:intergenic 25.0%
!! TTTAAACAGAACACTTTTCT+TGG - chr4_3:27647014-27647033 None:intergenic 25.0%
! TGTTCTGTTTAAATGAACTT+AGG + chr4_3:27647021-27647040 Msa0636740:exon 25.0%
! AGGATTAGAAAATAGTGATA+TGG + chr4_3:27647041-27647060 Msa0636740:three_prime_UTR 25.0%
!!! CTTTTCTAGAAACCTTTTAT+TGG - chr4_3:27647112-27647131 None:intergenic 25.0%
!! CTATTTGTGTAACCTTTTTA+TGG + chr4_3:27647292-27647311 Msa0636740:three_prime_UTR 25.0%
! TATAGCTATTCTCCATAAAA+AGG - chr4_3:27647307-27647326 None:intergenic 25.0%
AAATTGAAGGTAACGTTCTA+TGG - chr4_3:27643386-27643405 None:intergenic 30.0%
ACAAAGGGAAATGAAATTGA+AGG - chr4_3:27643399-27643418 None:intergenic 30.0%
ATTGATATGAGAAACAATGG+TGG - chr4_3:27643497-27643516 None:intergenic 30.0%
!! AGCATTGATATGAGAAACAA+TGG - chr4_3:27643500-27643519 None:intergenic 30.0%
ATATTATTTGGACATGTTGC+AGG - chr4_3:27643913-27643932 None:intergenic 30.0%
GAATTCATATCCGTTCATTT+AGG + chr4_3:27644050-27644069 Msa0636740:intron 30.0%
TCATTTATGCCCTAAATGAA+CGG - chr4_3:27644063-27644082 None:intergenic 30.0%
! TCATTTAGGGCATAAATGAA+CGG + chr4_3:27644064-27644083 Msa0636740:intron 30.0%
TTGCACCAAATACATCATTA+AGG - chr4_3:27644223-27644242 None:intergenic 30.0%
CTTGCAGAAAAAATAAGAGA+TGG + chr4_3:27644392-27644411 Msa0636740:intron 30.0%
AAAATAAGAGATGGAGGTAA+TGG + chr4_3:27644401-27644420 Msa0636740:CDS 30.0%
TAGATCACTCTATGTATTTC+AGG + chr4_3:27644516-27644535 Msa0636740:intron 30.0%
GGAAGATATTGGAATCATAA+AGG + chr4_3:27644537-27644556 Msa0636740:intron 30.0%
! CTTATAGATTTTCCATTGCT+TGG + chr4_3:27644575-27644594 Msa0636740:CDS 30.0%
ATATCACAAATGTATAAGGG+AGG - chr4_3:27644641-27644660 None:intergenic 30.0%
TCCTATTAACTATTTGCAGA+AGG + chr4_3:27644678-27644697 Msa0636740:intron 30.0%
TCCTTCTGCAAATAGTTAAT+AGG - chr4_3:27644682-27644701 None:intergenic 30.0%
TGTAGTAGTTTATTCCTTCA+CGG - chr4_3:27644725-27644744 None:intergenic 30.0%
ATTTATGATTGACAACCACA+TGG - chr4_3:27644816-27644835 None:intergenic 30.0%
! ATATGTGACTCTTTTCCATT+GGG + chr4_3:27644866-27644885 Msa0636740:CDS 30.0%
! ATCTTGCAGAGATGATTTTA+GGG + chr4_3:27645043-27645062 Msa0636740:intron 30.0%
ACTATACAAGACCAAATATC+AGG + chr4_3:27645286-27645305 Msa0636740:CDS 30.0%
GCATCTCAATATGGTATAAT+AGG + chr4_3:27645469-27645488 Msa0636740:CDS 30.0%
! TGCATTTTGCATTTTTCTGT+AGG + chr4_3:27645865-27645884 Msa0636740:intron 30.0%
! TGCATTTTTCTGTAGGTATA+TGG + chr4_3:27645872-27645891 Msa0636740:intron 30.0%
TATATGGATCCAATCACAAA+AGG + chr4_3:27645888-27645907 Msa0636740:CDS 30.0%
AAGAGGAAGAGTAATAGTTT+AGG - chr4_3:27646013-27646032 None:intergenic 30.0%
AATATATACCATTGGACCAA+GGG - chr4_3:27646262-27646281 None:intergenic 30.0%
AAATATATACCATTGGACCA+AGG - chr4_3:27646263-27646282 None:intergenic 30.0%
!! GCTTCTAGTTATTGATTTGT+AGG + chr4_3:27646737-27646756 Msa0636740:intron 30.0%
TATTGACCTTTATGCATAAC+AGG + chr4_3:27646818-27646837 Msa0636740:intron 30.0%
ATTGACCTTTATGCATAACA+GGG + chr4_3:27646819-27646838 Msa0636740:intron 30.0%
! ATGTAAAGGGATATTTTGCA+TGG + chr4_3:27646851-27646870 Msa0636740:CDS 30.0%
! ATATTTTGCATGGTCATTGT+TGG + chr4_3:27646861-27646880 Msa0636740:CDS 30.0%
ATTGTTGGATAACCATGAAT+GGG + chr4_3:27646876-27646895 Msa0636740:CDS 30.0%
!!! TTTCTTGGCAAGATTTTTTG+AGG - chr4_3:27646999-27647018 None:intergenic 30.0%
GTTCTATGGTTTCCCTTTAT+AGG - chr4_3:27643372-27643391 None:intergenic 35.0%
ATGGAAAAGGACCAAGTATT+TGG + chr4_3:27643783-27643802 Msa0636740:CDS 35.0%
TGGAAAAGGACCAAGTATTT+GGG + chr4_3:27643784-27643803 Msa0636740:CDS 35.0%
!! GTGAAAGTATCCCAAATACT+TGG - chr4_3:27643797-27643816 None:intergenic 35.0%
TTAAACTTAGTGCAACTTGC+TGG + chr4_3:27644192-27644211 Msa0636740:intron 35.0%
AGCAAAAGCTTATGCTATGT+TGG - chr4_3:27644301-27644320 None:intergenic 35.0%
TCCATTCATGCTATGTAACT+AGG - chr4_3:27644347-27644366 None:intergenic 35.0%
GCAGAAAAAATAAGAGATGG+AGG + chr4_3:27644395-27644414 Msa0636740:intron 35.0%
! ATCAACGAACTATTGGCTAA+TGG + chr4_3:27644753-27644772 Msa0636740:CDS 35.0%
CAATGGAAAAGAGTCACATA+TGG - chr4_3:27644867-27644886 None:intergenic 35.0%
! CATATGTGACTCTTTTCCAT+TGG + chr4_3:27644865-27644884 Msa0636740:CDS 35.0%
! GATCTTGCAGAGATGATTTT+AGG + chr4_3:27645042-27645061 Msa0636740:intron 35.0%
! CTACAAGGAATTTGGTGATA+GGG + chr4_3:27645082-27645101 Msa0636740:CDS 35.0%
GGATTACTTTGAATGAACCA+TGG + chr4_3:27645114-27645133 Msa0636740:CDS 35.0%
AGCATGAATGCTTATGCATA+TGG + chr4_3:27645143-27645162 Msa0636740:CDS 35.0%
TGGTTAAATCTGAATTGCAC+AGG + chr4_3:27645194-27645213 Msa0636740:CDS 35.0%
TTAAATCTGAATTGCACAGG+AGG + chr4_3:27645197-27645216 Msa0636740:CDS 35.0%
AAAACAGCAGACCTGATATT+TGG - chr4_3:27645300-27645319 None:intergenic 35.0%
TATTCGTAGCTCAACACTAA+CGG + chr4_3:27645603-27645622 Msa0636740:intron 35.0%
! GGATAGTTTCCTTTTGTGAT+TGG - chr4_3:27645900-27645919 None:intergenic 35.0%
! GATTCTTCTTTGGAGAACTT+TGG - chr4_3:27645957-27645976 None:intergenic 35.0%
GATAAGAGAGTCAGTTTGTA+TGG - chr4_3:27646075-27646094 None:intergenic 35.0%
ATATATACCATTGGACCAAG+GGG - chr4_3:27646261-27646280 None:intergenic 35.0%
TATTCTTGACGTAAAGCAAC+AGG - chr4_3:27646443-27646462 None:intergenic 35.0%
GTGTGACAAAATCCATTGAT+AGG + chr4_3:27646575-27646594 Msa0636740:intron 35.0%
ATTATGTTCAAGCTGCAATC+AGG + chr4_3:27646684-27646703 Msa0636740:CDS 35.0%
ATCAATAACTAGAAGCCTAG+AGG - chr4_3:27646733-27646752 None:intergenic 35.0%
CATTGTTGGATAACCATGAA+TGG + chr4_3:27646875-27646894 Msa0636740:CDS 35.0%
CTATGGTTTCCCTTTATAGG+AGG - chr4_3:27643369-27643388 None:intergenic 40.0%
GATATGAGAAACAATGGTGG+TGG - chr4_3:27643494-27643513 None:intergenic 40.0%
AACAGCTTCTTCTGCATTTC+AGG + chr4_3:27643600-27643619 Msa0636740:CDS 40.0%
GCAGCATTTGAAGATGGAAA+AGG + chr4_3:27643770-27643789 Msa0636740:CDS 40.0%
! GCTTTATGATGCAAGTTGGA+CGG - chr4_3:27644020-27644039 None:intergenic 40.0%
!! GTTGGCTTTATGATGCAAGT+TGG - chr4_3:27644024-27644043 None:intergenic 40.0%
GGATATGAATTCGCTACAGT+TGG - chr4_3:27644042-27644061 None:intergenic 40.0%
AGCTCCTATCTTTATGCTTG+TGG + chr4_3:27644095-27644114 Msa0636740:intron 40.0%
GCTCCTATCTTTATGCTTGT+GGG + chr4_3:27644096-27644115 Msa0636740:intron 40.0%
GATCCCACAAGCATAAAGAT+AGG - chr4_3:27644102-27644121 None:intergenic 40.0%
TAAGTTGCTTCTGAATGCCT+TGG + chr4_3:27644271-27644290 Msa0636740:intron 40.0%
TATGCTATGTTGGATCTCCA+AGG - chr4_3:27644291-27644310 None:intergenic 40.0%
GCCTAGTTACATAGCATGAA+TGG + chr4_3:27644343-27644362 Msa0636740:intron 40.0%
!! CAAGGTATGTAGTTTTGCAG+AGG + chr4_3:27644454-27644473 Msa0636740:intron 40.0%
TTTGCAGAAGGAAAGCTTAG+TGG + chr4_3:27644690-27644709 Msa0636740:intron 40.0%
CAACCTCATCAACGAACTAT+TGG + chr4_3:27644746-27644765 Msa0636740:CDS 40.0%
TAGCCAATAGTTCGTTGATG+AGG - chr4_3:27644752-27644771 None:intergenic 40.0%
!!! ATGACGGTTTTTTAAGCCCT+CGG + chr4_3:27644916-27644935 Msa0636740:CDS 40.0%
AAATCATCTCTGCAAGATCG+CGG - chr4_3:27645041-27645060 None:intergenic 40.0%
GACCTTTGCTACAAGGAATT+TGG + chr4_3:27645074-27645093 Msa0636740:CDS 40.0%
CACCAAATTCCTTGTAGCAA+AGG - chr4_3:27645079-27645098 None:intergenic 40.0%
! GCTACAAGGAATTTGGTGAT+AGG + chr4_3:27645081-27645100 Msa0636740:CDS 40.0%
! TTGGTGATAGGGTGAAACAT+TGG + chr4_3:27645093-27645112 Msa0636740:CDS 40.0%
TGCATATGGAAAGTTTGCAC+CGG + chr4_3:27645157-27645176 Msa0636740:CDS 40.0%
GAAATGCAGGCATCTCAATA+TGG + chr4_3:27645460-27645479 Msa0636740:intron 40.0%
GGATTACCTTACTCTCTCAT+TGG + chr4_3:27645491-27645510 Msa0636740:CDS 40.0%
CTCATACCAATGAGAGAGTA+AGG - chr4_3:27645500-27645519 None:intergenic 40.0%
! TCCAAAAAGCATGCGATCTT+TGG + chr4_3:27645917-27645936 Msa0636740:CDS 40.0%
! ACCAAAGATCGCATGCTTTT+TGG - chr4_3:27645921-27645940 None:intergenic 40.0%
! AAAAGCATGCGATCTTTGGT+TGG + chr4_3:27645921-27645940 Msa0636740:CDS 40.0%
AAGATCCTCGGATTCTTCTT+TGG - chr4_3:27645967-27645986 None:intergenic 40.0%
AGAAGAATCCGAGGATCTTA+AGG + chr4_3:27645968-27645987 Msa0636740:CDS 40.0%
GAAGAATCCGAGGATCTTAA+GGG + chr4_3:27645969-27645988 Msa0636740:CDS 40.0%
AAGAATCCGAGGATCTTAAG+GGG + chr4_3:27645970-27645989 Msa0636740:CDS 40.0%
GTTTAGGCCAAGAAAATCGA+AGG - chr4_3:27645997-27646016 None:intergenic 40.0%
ATATGCAGTCTGCTTCAAGT+TGG + chr4_3:27646388-27646407 Msa0636740:intron 40.0%
TGGTTATGCATTTATCCGAG+AGG + chr4_3:27646408-27646427 Msa0636740:CDS 40.0%
TGCATTTATCCGAGAGGATT+AGG + chr4_3:27646414-27646433 Msa0636740:CDS 40.0%
AACAGGTTTCCTAATCCTCT+CGG - chr4_3:27646426-27646445 None:intergenic 40.0%
! GATTCTTCGAGTGACAGTTT+TGG - chr4_3:27646620-27646639 None:intergenic 40.0%
ACCTTTATGCATAACAGGGA+AGG + chr4_3:27646823-27646842 Msa0636740:intron 40.0%
ACCTTCCCTGTTATGCATAA+AGG - chr4_3:27646827-27646846 None:intergenic 40.0%
! AGGGAAGGTGTGAATGTAAA+GGG + chr4_3:27646838-27646857 Msa0636740:intron 40.0%
GATAACCATGAATGGGAATC+TGG + chr4_3:27646883-27646902 Msa0636740:CDS 40.0%
GAAAAGACACCCAAAACTCT+CGG + chr4_3:27646954-27646973 Msa0636740:CDS 40.0%
TTCCCTTTATAGGAGGAGGA+GGG - chr4_3:27643362-27643381 None:intergenic 45.0%
TTTCCCTTTATAGGAGGAGG+AGG - chr4_3:27643363-27643382 None:intergenic 45.0%
TGGTTTCCCTTTATAGGAGG+AGG - chr4_3:27643366-27643385 None:intergenic 45.0%
!! AACAATGGTGGTGGCGATAA+TGG - chr4_3:27643485-27643504 None:intergenic 45.0%
CGAAGATGGAGTAGTGAAGA+AGG - chr4_3:27643529-27643548 None:intergenic 45.0%
! GCGACTTTTCCGATCTCAAT+CGG + chr4_3:27643548-27643567 Msa0636740:CDS 45.0%
GAAACAGGTCCGATTGAGAT+CGG - chr4_3:27643560-27643579 None:intergenic 45.0%
! GAAGGTGCAGCATTTGAAGA+TGG + chr4_3:27643764-27643783 Msa0636740:CDS 45.0%
ACTTTCACTCATGAACACCC+AGG + chr4_3:27643809-27643828 Msa0636740:CDS 45.0%
GGCATAAATGAACGGACATG+TGG + chr4_3:27644072-27644091 Msa0636740:intron 45.0%
TAGCACTCTAGACCAAGCAA+TGG - chr4_3:27644590-27644609 None:intergenic 45.0%
!! TACTCATCCTCTAAGGCTTG+AGG - chr4_3:27644897-27644916 None:intergenic 45.0%
GCCTTAGAGGATGAGTATGA+CGG + chr4_3:27644900-27644919 Msa0636740:CDS 45.0%
ACCGTCATACTCATCCTCTA+AGG - chr4_3:27644904-27644923 None:intergenic 45.0%
GCATATGGAAAGTTTGCACC+GGG + chr4_3:27645158-27645177 Msa0636740:CDS 45.0%
AACCAATCAGAACATCGACC+CGG - chr4_3:27645179-27645198 None:intergenic 45.0%
GAATTGCACAGGAGGTGATT+CGG + chr4_3:27645205-27645224 Msa0636740:CDS 45.0%
AATTGCACAGGAGGTGATTC+GGG + chr4_3:27645206-27645225 Msa0636740:CDS 45.0%
! TGGTAGTGCGCAGTCAAATA+CGG - chr4_3:27645239-27645258 None:intergenic 45.0%
ACTCTCTCATTGGTATGAGC+CGG + chr4_3:27645501-27645520 Msa0636740:CDS 45.0%
AACATCTGCTTTCGCTTGAG+AGG - chr4_3:27645527-27645546 None:intergenic 45.0%
ACCATCCAAACATGAAGTCG+AGG - chr4_3:27645567-27645586 None:intergenic 45.0%
! CTTTGGAGAACTTTGGTAGC+CGG - chr4_3:27645950-27645969 None:intergenic 45.0%
GTTCTCCAAAGAAGAATCCG+AGG + chr4_3:27645959-27645978 Msa0636740:CDS 45.0%
! AAGGGGTCCTTCGATTTTCT+TGG + chr4_3:27645987-27646006 Msa0636740:CDS 45.0%
AGAGAGTCAGTTTGTATGGC+TGG - chr4_3:27646071-27646090 None:intergenic 45.0%
TCGATGACAGTTCATCGTGA+TGG + chr4_3:27646231-27646250 Msa0636740:intron 45.0%
AACTCATCGCGACCTATCAA+TGG - chr4_3:27646590-27646609 None:intergenic 45.0%
GCTTTATCTGAAGAGCCTCT+AGG + chr4_3:27646715-27646734 Msa0636740:intron 45.0%
! CAGGGAAGGTGTGAATGTAA+AGG + chr4_3:27646837-27646856 Msa0636740:intron 45.0%
TGAAGCCAGATTCCCATTCA+TGG - chr4_3:27646891-27646910 None:intergenic 45.0%
! ACCAATGTGCCGAGAGTTTT+GGG - chr4_3:27646966-27646985 None:intergenic 45.0%
! AACCAATGTGCCGAGAGTTT+TGG - chr4_3:27646967-27646986 None:intergenic 45.0%
!! AATAACATATTAATATTATT+TGG - chr4_3:27643925-27643944 None:intergenic 5.0%
!!! ATTTTTTTTTTTTTTTGAAA+AGG - chr4_3:27644158-27644177 None:intergenic 5.0%
!!! ATATTTTAGAAATAATATTT+TGG - chr4_3:27646345-27646364 None:intergenic 5.0%
!!! TTTAGTTTTAATTTTTTTAA+TGG + chr4_3:27647153-27647172 Msa0636740:three_prime_UTR 5.0%
CTCCCTCCTCCTCCTATAAA+GGG + chr4_3:27643357-27643376 Msa0636740:five_prime_UTR 50.0%
GATCGGAAAAGTCGCGAAGA+TGG - chr4_3:27643543-27643562 None:intergenic 50.0%
!!! TTCCCGCCAGGCTTTGTTTT+CGG + chr4_3:27643577-27643596 Msa0636740:CDS 50.0%
TTCCGAAAACAAAGCCTGGC+GGG - chr4_3:27643582-27643601 None:intergenic 50.0%
GTTCCGAAAACAAAGCCTGG+CGG - chr4_3:27643583-27643602 None:intergenic 50.0%
GCTGTTCCGAAAACAAAGCC+TGG - chr4_3:27643586-27643605 None:intergenic 50.0%
TAGCCGACGACGCATATCAT+CGG + chr4_3:27644429-27644448 Msa0636740:CDS 50.0%
! GTACCGATGATATGCGTCGT+CGG - chr4_3:27644435-27644454 None:intergenic 50.0%
CGACGCATATCATCGGTACA+AGG + chr4_3:27644436-27644455 Msa0636740:CDS 50.0%
GCAGAAGGAAAGCTTAGTGG+AGG + chr4_3:27644693-27644712 Msa0636740:intron 50.0%
! AGTGGAGGTGTAAACCGTGA+AGG + chr4_3:27644708-27644727 Msa0636740:CDS 50.0%
! GTGTCACTCACACAATCCGA+GGG - chr4_3:27644935-27644954 None:intergenic 50.0%
CTACGCTGACCTTTGCTACA+AGG + chr4_3:27645067-27645086 Msa0636740:CDS 50.0%
GCATTCATGCTCACACTCCA+TGG - chr4_3:27645134-27645153 None:intergenic 50.0%
ATTGCACAGGAGGTGATTCG+GGG + chr4_3:27645207-27645226 Msa0636740:CDS 50.0%
CAGCAGCATGAGCAAGAAGT+TGG - chr4_3:27645259-27645278 None:intergenic 50.0%
GATGTTGATGCTGCACAACG+AGG + chr4_3:27645541-27645560 Msa0636740:CDS 50.0%
GCCTCGACTTCATGTTTGGA+TGG + chr4_3:27645563-27645582 Msa0636740:CDS 50.0%
TGCGATCTTTGGTTGGAAGC+CGG + chr4_3:27645928-27645947 Msa0636740:CDS 50.0%
GAAGGACCCCTTAAGATCCT+CGG - chr4_3:27645979-27645998 None:intergenic 50.0%
AGGTGCATCAGCAGCATAAG+AGG - chr4_3:27646030-27646049 None:intergenic 50.0%
! GTCAGTTTGTATGGCTGGTC+TGG - chr4_3:27646066-27646085 None:intergenic 50.0%
! TCTGGCTTCAGCTTGCGATT+TGG + chr4_3:27646901-27646920 Msa0636740:CDS 50.0%
! GCGATTTGGACTCGTCTTTG+TGG + chr4_3:27646915-27646934 Msa0636740:CDS 50.0%
ACCCAAAACTCTCGGCACAT+TGG + chr4_3:27646962-27646981 Msa0636740:CDS 50.0%
CCTTTATAGGAGGAGGAGGG+AGG - chr4_3:27643359-27643378 None:intergenic 55.0%
CCTCCCTCCTCCTCCTATAA+AGG + chr4_3:27643356-27643375 Msa0636740:five_prime_UTR 55.0%
AACAAAGCCTGGCGGGAAAC+AGG - chr4_3:27643575-27643594 None:intergenic 55.0%
AGGCTTGAGGAACGTCCCAA+TGG - chr4_3:27644884-27644903 None:intergenic 55.0%
GGACGTTCCTCAAGCCTTAG+AGG + chr4_3:27644887-27644906 Msa0636740:CDS 55.0%
!! GGTGTCACTCACACAATCCG+AGG - chr4_3:27644936-27644955 None:intergenic 55.0%
CACCGGGTCGATGTTCTGAT+TGG + chr4_3:27645174-27645193 Msa0636740:CDS 55.0%
TCTGCTTTCGCTTGAGAGGC+CGG - chr4_3:27645523-27645542 None:intergenic 55.0%
CGAGGCCTCGACTTCATGTT+TGG + chr4_3:27645559-27645578 Msa0636740:CDS 55.0%
AATCGGACCTGTTTCCCGCC+AGG + chr4_3:27643565-27643584 Msa0636740:CDS 60.0%
GGTCTGGCATCGCGTAAGTG+AGG - chr4_3:27646050-27646069 None:intergenic 60.0%
CATCGTGATGGCAAGCCCCT+TGG + chr4_3:27646243-27646262 Msa0636740:CDS 60.0%
TGGCAAGCCCCTTGGTCCAA+TGG + chr4_3:27646251-27646270 Msa0636740:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 27643261 27647326 27643261 ID=Msa0636740;Name=Msa0636740
chr4_3 mRNA 27643261 27647326 27643261 ID=Msa0636740-mRNA-1;Parent=Msa0636740;Name=Msa0636740-mRNA-1;_AED=0.16;_eAED=0.16;_QI=175|1|1|1|1|1|13|294|527
chr4_3 exon 27643261 27643621 27643261 ID=Msa0636740-mRNA-1:exon:6829;Parent=Msa0636740-mRNA-1
chr4_3 exon 27643761 27643830 27643761 ID=Msa0636740-mRNA-1:exon:6830;Parent=Msa0636740-mRNA-1
chr4_3 exon 27644399 27644457 27644399 ID=Msa0636740-mRNA-1:exon:6831;Parent=Msa0636740-mRNA-1
chr4_3 exon 27644538 27644613 27644538 ID=Msa0636740-mRNA-1:exon:6832;Parent=Msa0636740-mRNA-1
chr4_3 exon 27644697 27644774 27644697 ID=Msa0636740-mRNA-1:exon:6833;Parent=Msa0636740-mRNA-1
chr4_3 exon 27644856 27644943 27644856 ID=Msa0636740-mRNA-1:exon:6834;Parent=Msa0636740-mRNA-1
chr4_3 exon 27645052 27645307 27645052 ID=Msa0636740-mRNA-1:exon:6835;Parent=Msa0636740-mRNA-1
chr4_3 exon 27645469 27645584 27645469 ID=Msa0636740-mRNA-1:exon:6836;Parent=Msa0636740-mRNA-1
chr4_3 exon 27645887 27646104 27645887 ID=Msa0636740-mRNA-1:exon:6837;Parent=Msa0636740-mRNA-1
chr4_3 exon 27646241 27646272 27646241 ID=Msa0636740-mRNA-1:exon:6838;Parent=Msa0636740-mRNA-1
chr4_3 exon 27646396 27646498 27646396 ID=Msa0636740-mRNA-1:exon:6839;Parent=Msa0636740-mRNA-1
chr4_3 exon 27646597 27646705 27646597 ID=Msa0636740-mRNA-1:exon:6840;Parent=Msa0636740-mRNA-1
chr4_3 exon 27646840 27647326 27646840 ID=Msa0636740-mRNA-1:exon:6841;Parent=Msa0636740-mRNA-1
chr4_3 five_prime_UTR 27643261 27643435 27643261 ID=Msa0636740-mRNA-1:five_prime_utr;Parent=Msa0636740-mRNA-1
chr4_3 CDS 27643436 27643621 27643436 ID=Msa0636740-mRNA-1:cds;Parent=Msa0636740-mRNA-1
chr4_3 CDS 27643761 27643830 27643761 ID=Msa0636740-mRNA-1:cds;Parent=Msa0636740-mRNA-1
chr4_3 CDS 27644399 27644457 27644399 ID=Msa0636740-mRNA-1:cds;Parent=Msa0636740-mRNA-1
chr4_3 CDS 27644538 27644613 27644538 ID=Msa0636740-mRNA-1:cds;Parent=Msa0636740-mRNA-1
chr4_3 CDS 27644697 27644774 27644697 ID=Msa0636740-mRNA-1:cds;Parent=Msa0636740-mRNA-1
chr4_3 CDS 27644856 27644943 27644856 ID=Msa0636740-mRNA-1:cds;Parent=Msa0636740-mRNA-1
chr4_3 CDS 27645052 27645307 27645052 ID=Msa0636740-mRNA-1:cds;Parent=Msa0636740-mRNA-1
chr4_3 CDS 27645469 27645584 27645469 ID=Msa0636740-mRNA-1:cds;Parent=Msa0636740-mRNA-1
chr4_3 CDS 27645887 27646104 27645887 ID=Msa0636740-mRNA-1:cds;Parent=Msa0636740-mRNA-1
chr4_3 CDS 27646241 27646272 27646241 ID=Msa0636740-mRNA-1:cds;Parent=Msa0636740-mRNA-1
chr4_3 CDS 27646396 27646498 27646396 ID=Msa0636740-mRNA-1:cds;Parent=Msa0636740-mRNA-1
chr4_3 CDS 27646597 27646705 27646597 ID=Msa0636740-mRNA-1:cds;Parent=Msa0636740-mRNA-1
chr4_3 CDS 27646840 27647032 27646840 ID=Msa0636740-mRNA-1:cds;Parent=Msa0636740-mRNA-1
chr4_3 three_prime_UTR 27647033 27647326 27647033 ID=Msa0636740-mRNA-1:three_prime_utr;Parent=Msa0636740-mRNA-1
Gene Sequence

>Msa0636740

ATGGCAGCATTTCATTTCAACGTGTTTTTGTTACTCTTCATTTTTTCCATTATCGCCACCACCATTGTTTCTCATATCAATGCTTTTGAACCTTCTTCACTACTCCATCTTCGCGACTTTTCCGATCTCAATCGGACCTGTTTCCCGCCAGGCTTTGTTTTCGGAACAGCTTCTTCTGCATTTCAGTATGAAGGTGCAGCATTTGAAGATGGAAAAGGACCAAGTATTTGGGATACTTTCACTCATGAACACCCAGAAAAAATAAGAGATGGAGGTAATGGAGATGTAGCCGACGACGCATATCATCGGTACAAGGAAGATATTGGAATCATAAAGGATTTGAATATGGATGCTTATAGATTTTCCATTGCTTGGTCTAGAGTGCTACCAAAAGGAAAGCTTAGTGGAGGTGTAAACCGTGAAGGAATAAACTACTACAACAACCTCATCAACGAACTATTGGCTAATGGTTTGCAGCCATATGTGACTCTTTTCCATTGGGACGTTCCTCAAGCCTTAGAGGATGAGTATGACGGTTTTTTAAGCCCTCGGATTGTAGATGATTTTAGGGACTACGCTGACCTTTGCTACAAGGAATTTGGTGATAGGGTGAAACATTGGATTACTTTGAATGAACCATGGAGTGTGAGCATGAATGCTTATGCATATGGAAAGTTTGCACCGGGTCGATGTTCTGATTGGTTAAATCTGAATTGCACAGGAGGTGATTCGGGGACAGAACCGTATTTGACTGCGCACTACCAACTTCTTGCTCATGCTGCTGCTACAAAACTATACAAGACCAAATATCAGGCATCTCAATATGGTATAATAGGGATTACCTTACTCTCTCATTGGTATGAGCCGGCCTCTCAAGCGAAAGCAGATGTTGATGCTGCACAACGAGGCCTCGACTTCATGTTTGGATGGTATATGGATCCAATCACAAAAGGAAACTATCCAAAAAGCATGCGATCTTTGGTTGGAAGCCGGCTACCAAAGTTCTCCAAAGAAGAATCCGAGGATCTTAAGGGGTCCTTCGATTTTCTTGGCCTAAACTATTACTCTTCCTCTTATGCTGCTGATGCACCTCACTTACGCGATGCCAGACCAGCCATACAAACTGACTCTCTTATCAATGCTACATTTCATCGTGATGGCAAGCCCCTTGGTCCAATGTCTGCTTCAAGTTGGTTATGCATTTATCCGAGAGGATTAGGAAACCTGTTGCTTTACGTCAAGAATAAGTACGACAACCCCATAATATACATTACTGAAAATGGTCGCGATGAGTTCAACGATCCAAAACTGTCACTCGAAGAATCCCTTTTAGATACTTATAGAATCGATTACTTTTATCGTCATCTTTATTATGTTCAAGCTGCAATCAGGGAAGGTGTGAATGTAAAGGGATATTTTGCATGGTCATTGTTGGATAACCATGAATGGGAATCTGGCTTCAGCTTGCGATTTGGACTCGTCTTTGTGGATTATAATGACGACTTGAAAAGACACCCAAAACTCTCGGCACATTGGTTCAAGAATTTCCTCAAAAAATCTTGCCAAGAAAAGTGTTCTGTTTAA
Protein sequence

>Msa0636740

MAAFHFNVFLLLFIFSIIATTIVSHINAFEPSSLLHLRDFSDLNRTCFPPGFVFGTASSAFQYEGAAFEDGKGPSIWDTFTHEHPEKIRDGGNGDVADDAYHRYKEDIGIIKDLNMDAYRFSIAWSRVLPKGKLSGGVNREGINYYNNLINELLANGLQPYVTLFHWDVPQALEDEYDGFLSPRIVDDFRDYADLCYKEFGDRVKHWITLNEPWSVSMNAYAYGKFAPGRCSDWLNLNCTGGDSGTEPYLTAHYQLLAHAAATKLYKTKYQASQYGIIGITLLSHWYEPASQAKADVDAAQRGLDFMFGWYMDPITKGNYPKSMRSLVGSRLPKFSKEESEDLKGSFDFLGLNYYSSSYAADAPHLRDARPAIQTDSLINATFHRDGKPLGPMSASSWLCIYPRGLGNLLLYVKNKYDNPIIYITENGRDEFNDPKLSLEESLLDTYRIDYFYRHLYYVQAAIREGVNVKGYFAWSLLDNHEWESGFSLRFGLVFVDYNDDLKRHPKLSAHWFKNFLKKSCQEKCSV*