AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0645450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0645450 MtrunA17_Chr4g0033191 96.964 527 16 0 1 527 1 527 0.0 1069
Msa0645450 MtrunA17_Chr4g0033201 85.000 520 71 2 1 520 1 513 0.0 920
Msa0645450 MtrunA17_Chr4g0033271 76.008 521 115 3 1 520 1 512 0.0 826
Msa0645450 MtrunA17_Chr4g0033311 65.577 520 176 1 3 522 2 518 0.0 741
Msa0645450 MtrunA17_Chr3g0088441 64.217 517 183 1 6 522 3 517 0.0 729
Msa0645450 MtrunA17_Chr4g0033401 64.990 517 172 2 1 517 1 508 0.0 717
Msa0645450 MtrunA17_Chr6g0482131 64.147 516 179 2 7 521 4 514 0.0 717
Msa0645450 MtrunA17_Chr4g0033331 69.053 475 147 0 43 517 39 513 0.0 716
Msa0645450 MtrunA17_Chr2g0329201 64.648 512 174 2 8 519 3 507 0.0 716
Msa0645450 MtrunA17_Chr4g0033321 64.648 512 178 2 13 523 4 513 0.0 715
Msa0645450 MtrunA17_Chr6g0482071 64.272 515 180 1 5 519 3 513 0.0 707
Msa0645450 MtrunA17_Chr2g0329191 66.460 483 161 1 37 519 28 509 0.0 705
Msa0645450 MtrunA17_Chr6g0482081 66.472 513 168 3 8 518 2 512 0.0 700
Msa0645450 MtrunA17_Chr4g0033341 61.240 516 191 4 6 520 37 544 0.0 682
Msa0645450 MtrunA17_Chr2g0329321 61.868 514 163 2 6 519 8 488 0.0 666
Msa0645450 MtrunA17_Chr4g0033261 60.348 517 176 3 6 519 3 493 0.0 645
Msa0645450 MtrunA17_Chr4g0033231 59.127 504 175 3 18 519 19 493 0.0 632
Msa0645450 MtrunA17_Chr5g0430451 58.891 523 179 3 6 519 3 498 0.0 610
Msa0645450 MtrunA17_Chr4g0010501 58.491 477 192 3 43 519 24 494 0.0 581
Msa0645450 MtrunA17_Chr4g0006111 53.955 493 187 6 39 519 61 525 0.0 551
Msa0645450 MtrunA17_Chr3g0087261 51.373 510 239 6 11 519 8 509 0.0 547
Msa0645450 MtrunA17_Chr4g0006131 53.800 500 192 9 30 518 29 500 0.0 533
Msa0645450 MtrunA17_Chr4g0043091 53.512 484 212 5 41 518 26 502 0.0 526
Msa0645450 MtrunA17_Chr3g0087271 50.867 519 243 5 1 519 4 510 0.0 526
Msa0645450 MtrunA17_Chr6g0482941 48.438 512 252 8 9 519 12 512 3.48e-168 486
Msa0645450 MtrunA17_Chr6g0482951 48.242 512 253 8 9 519 12 512 9.67e-168 484
Msa0645450 MtrunA17_Chr4g0042171 49.385 488 239 5 43 526 24 507 1.57e-163 474
Msa0645450 MtrunA17_Chr4g0074561 49.260 473 235 5 48 517 40 510 7.10e-161 468
Msa0645450 MtrunA17_Chr2g0301231 55.370 419 150 5 111 519 1 392 1.94e-159 459
Msa0645450 MtrunA17_Chr2g0288071 47.490 518 251 7 8 523 48 546 5.57e-159 464
Msa0645450 MtrunA17_Chr4g0074551 48.536 478 240 6 48 523 55 528 1.29e-155 455
Msa0645450 MtrunA17_Chr3g0090131 46.507 501 243 7 27 524 11 489 3.99e-154 449
Msa0645450 MtrunA17_Chr3g0090111 46.901 484 239 6 43 524 19 486 6.72e-154 448
Msa0645450 MtrunA17_Chr2g0317821 45.090 499 259 7 33 523 18 509 3.98e-150 440
Msa0645450 MtrunA17_Chr2g0317841 44.489 499 262 7 33 523 18 509 4.78e-149 437
Msa0645450 MtrunA17_Chr2g0288021 42.607 514 277 7 8 519 12 509 2.62e-147 432
Msa0645450 MtrunA17_Chr7g0242691 45.155 485 238 7 43 519 31 495 3.04e-146 430
Msa0645450 MtrunA17_Chr7g0242681 46.281 484 231 9 43 518 38 500 9.50e-144 424
Msa0645450 MtrunA17_Chr4g0006971 44.008 484 253 7 45 525 29 497 2.38e-139 412
Msa0645450 MtrunA17_Chr5g0428831 44.491 481 246 8 44 520 28 491 4.59e-138 409
Msa0645450 MtrunA17_Chr1g0195611 43.892 483 249 7 42 520 26 490 2.12e-134 399
Msa0645450 MtrunA17_Chr3g0087201 53.754 333 149 2 187 519 17 344 5.83e-128 377
Msa0645450 MtrunA17_Chr7g0234091 44.835 484 241 8 44 523 76 537 1.46e-126 380
Msa0645450 MtrunA17_Chr7g0234111 45.168 476 239 6 44 517 76 531 4.84e-125 376
Msa0645450 MtrunA17_Chr7g0234131 43.277 476 249 5 44 517 76 532 1.23e-122 370
Msa0645450 MtrunA17_Chr5g0418871 55.667 300 130 2 61 360 1 297 5.33e-120 356
Msa0645450 MtrunA17_Chr1g0195641 46.770 387 191 6 45 428 29 403 2.71e-114 344
Msa0645450 MtrunA17_Chr7g0230651 44.267 375 193 5 90 464 18 376 2.89e-101 310
Msa0645450 MtrunA17_Chr3g0087191 69.375 160 42 1 34 186 6 165 1.84e-77 241
Msa0645450 MtrunA17_Chr7g0236731 48.085 235 115 2 132 365 28 256 1.06e-61 204
Msa0645450 MtrunA17_Chr7g0227561 36.656 311 157 6 9 313 7 283 4.03e-60 200
Msa0645450 MtrunA17_Chr4g0068871 27.098 417 231 12 107 517 183 532 2.08e-28 119
Msa0645450 MtrunA17_Chr5g0418861 58.427 89 36 1 430 517 2 90 1.07e-27 106
Msa0645450 MtrunA17_Chr8g0352881 45.000 100 55 0 269 368 57 156 1.18e-23 97.4
Msa0645450 MtrunA17_Chr8g0352881 48.571 70 35 1 52 121 7 75 1.17e-12 66.2
Msa0645450 MtrunA17_Chr7g0227591 54.386 57 26 0 465 521 13 69 1.74e-14 68.6
Msa0645450 MtrunA17_Chr2g0288031 46.552 58 30 1 195 252 4 60 1.18e-11 60.1
Msa0645450 MtrunA17_Chr2g0301221 72.222 36 10 0 48 83 70 105 4.37e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0645450 AT5G44640.1 51.859 511 230 4 10 519 8 503 0.0 565
Msa0645450 AT5G42260.1 52.756 508 226 5 7 513 3 497 0.0 561
Msa0645450 AT2G44480.3 53.144 493 227 2 28 520 54 542 0.0 561
Msa0645450 AT2G44480.1 53.144 493 227 2 28 520 23 511 0.0 560
Msa0645450 AT2G44450.1 54.184 478 212 3 43 519 31 502 0.0 560
Msa0645450 AT3G60130.1 54.507 477 211 3 43 518 30 501 0.0 541
Msa0645450 AT2G25630.1 51.176 510 217 6 9 517 5 483 0.0 530
Msa0645450 AT5G24550.1 50.688 509 245 6 17 520 5 512 0.0 525
Msa0645450 AT1G26560.1 50.413 484 232 6 41 520 30 509 1.89e-175 504
Msa0645450 AT5G24540.1 50.884 509 244 6 17 520 5 512 2.05e-175 505
Msa0645450 AT3G60140.1 47.868 516 249 6 9 521 8 506 3.50e-175 506
Msa0645450 AT2G44460.1 49.614 518 247 7 10 523 3 510 1.29e-171 497
Msa0645450 AT2G44470.3 47.002 517 255 6 10 519 3 507 4.26e-171 496
Msa0645450 AT5G54570.1 51.566 479 225 5 43 517 30 505 1.09e-170 493
Msa0645450 AT1G51470.1 49.692 487 231 6 35 519 37 511 3.77e-170 491
Msa0645450 AT1G47600.1 49.491 491 226 7 35 519 37 511 7.05e-169 488
Msa0645450 AT1G47600.2 49.287 491 226 7 35 519 37 510 6.84e-168 485
Msa0645450 AT3G60120.1 50.309 485 230 7 41 519 12 491 1.13e-167 486
Msa0645450 AT3G18080.1 49.583 480 232 7 43 520 39 510 4.54e-165 478
Msa0645450 AT2G44490.1 49.269 479 237 5 45 518 16 493 2.95e-164 478
Msa0645450 AT2G44480.2 52.195 410 195 1 111 520 1 409 6.40e-163 469
Msa0645450 AT5G25980.2 46.360 522 260 11 12 524 19 529 1.54e-158 462
Msa0645450 AT3G60130.2 53.012 415 189 3 105 518 40 449 1.87e-158 459
Msa0645450 AT3G18070.3 46.875 480 235 9 43 519 30 492 1.29e-157 458
Msa0645450 AT5G36890.1 48.682 493 240 4 33 524 6 486 5.66e-157 457
Msa0645450 AT2G32860.2 46.107 488 259 4 42 525 93 580 7.17e-157 461
Msa0645450 AT5G36890.2 48.682 493 240 4 33 524 6 486 8.81e-157 456
Msa0645450 AT3G18070.1 46.875 480 241 9 43 519 30 498 3.62e-156 455
Msa0645450 AT3G03640.1 44.874 517 271 6 8 519 7 514 1.54e-155 454
Msa0645450 AT2G32860.1 46.107 488 258 5 42 525 93 579 1.70e-155 457
Msa0645450 AT5G26000.1 46.080 523 255 10 8 517 3 511 9.73e-155 453
Msa0645450 AT1G61820.1 48.276 493 246 7 32 521 22 508 6.56e-154 450
Msa0645450 AT1G66280.1 44.592 527 275 8 1 522 1 515 1.14e-149 439
Msa0645450 AT3G60130.3 51.566 415 184 4 105 518 40 438 1.85e-149 436
Msa0645450 AT3G09260.1 45.963 483 256 5 43 522 35 515 2.04e-149 439
Msa0645450 AT1G02850.2 46.804 485 231 8 44 522 27 490 8.00e-149 436
Msa0645450 AT2G44470.1 46.567 466 227 6 4 463 1 450 2.00e-148 433
Msa0645450 AT5G24540.2 49.446 451 223 5 17 463 5 454 3.85e-148 433
Msa0645450 AT1G75940.1 46.170 483 250 7 43 517 37 517 4.69e-148 436
Msa0645450 AT5G54570.3 51.294 425 201 4 43 464 30 451 1.05e-147 431
Msa0645450 AT1G02850.5 45.059 506 228 9 44 520 27 511 4.13e-144 425
Msa0645450 AT5G28510.1 43.992 491 259 7 43 522 39 524 9.06e-144 424
Msa0645450 AT3G21370.1 44.969 487 250 9 43 518 33 512 1.42e-143 424
Msa0645450 AT3G60140.2 47.418 426 205 5 9 432 8 416 3.32e-143 420
Msa0645450 AT1G52400.3 44.146 521 275 11 6 518 4 516 1.59e-142 421
Msa0645450 AT1G52400.1 44.146 521 275 11 6 518 4 516 1.59e-142 421
Msa0645450 AT1G66270.1 44.856 486 260 6 41 522 34 515 1.83e-142 421
Msa0645450 AT1G02850.4 44.970 507 228 10 44 520 27 512 2.07e-142 421
Msa0645450 AT4G21760.2 44.280 472 252 6 48 517 59 521 3.28e-142 421
Msa0645450 AT1G61810.1 42.278 518 289 6 10 524 4 514 3.71e-142 420
Msa0645450 AT4G27830.1 42.276 492 263 7 34 521 14 488 1.35e-140 416
Msa0645450 AT5G54570.2 50.122 411 198 4 111 517 1 408 1.49e-140 413
Msa0645450 AT5G25980.3 46.522 460 228 9 12 464 19 467 2.69e-139 410
Msa0645450 AT5G25980.1 46.522 460 228 9 12 464 19 467 2.69e-139 410
Msa0645450 AT1G66270.2 44.650 486 259 7 41 522 34 513 2.76e-139 412
Msa0645450 AT1G02850.1 45.567 485 210 9 44 522 27 463 4.39e-139 410
Msa0645450 AT2G44480.4 57.055 326 135 3 28 353 54 374 9.15e-137 401
Msa0645450 AT4G22100.1 43.867 481 244 9 44 518 23 483 6.79e-136 403
Msa0645450 AT1G60090.2 43.035 481 248 9 43 518 24 483 1.00e-135 403
Msa0645450 AT4G21760.1 43.644 472 245 7 48 517 59 511 1.24e-135 404
Msa0645450 AT5G26000.2 44.776 469 236 7 6 464 1 456 1.67e-135 400
Msa0645450 AT1G61810.3 42.570 498 276 6 10 504 4 494 3.22e-135 403
Msa0645450 AT2G44450.2 52.101 357 164 3 164 519 1 351 1.10e-133 392
Msa0645450 AT1G60090.1 42.355 484 254 10 43 518 24 490 3.39e-132 394
Msa0645450 AT4G27820.1 42.236 483 256 7 43 521 23 486 2.34e-131 392
Msa0645450 AT1G45191.4 42.292 480 254 9 41 518 26 484 3.87e-129 386
Msa0645450 AT2G44470.2 47.906 382 185 4 12 387 5 378 4.74e-129 382
Msa0645450 AT1G45191.2 41.822 483 258 9 41 518 26 490 1.03e-126 380
Msa0645450 AT1G45191.6 41.309 489 257 9 41 518 26 495 5.11e-126 379
Msa0645450 AT3G62750.1 40.039 517 260 12 5 517 3 473 1.09e-125 377
Msa0645450 AT3G62750.2 39.731 521 264 12 5 521 29 503 2.79e-125 377
Msa0645450 AT1G45191.1 41.176 493 264 9 41 518 26 507 3.52e-123 372
Msa0645450 AT1G52400.2 43.441 465 248 10 6 463 4 460 4.10e-119 359
Msa0645450 AT1G02850.3 45.278 413 183 6 44 428 27 424 2.25e-118 356
Msa0645450 AT3G62740.1 37.079 534 280 12 9 526 4 497 3.75e-118 358
Msa0645450 AT5G48375.1 41.372 452 204 7 61 510 47 439 1.14e-116 352
Msa0645450 AT1G61810.5 42.754 414 231 5 114 524 4 414 1.59e-114 345
Msa0645450 AT2G44460.2 49.180 366 180 5 162 523 1 364 4.57e-113 342
Msa0645450 AT4G27830.2 41.205 415 225 6 111 521 1 400 4.98e-112 339
Msa0645450 AT1G61810.4 41.232 422 239 5 10 428 4 419 1.23e-110 336
Msa0645450 AT3G18070.4 49.062 320 157 4 43 362 30 343 1.17e-109 332
Msa0645450 AT1G60270.1 49.684 316 151 4 41 355 21 329 2.09e-108 328
Msa0645450 AT3G62750.4 40.215 465 229 11 5 466 29 447 1.25e-107 329
Msa0645450 AT1G61820.3 45.380 368 196 4 157 521 4 369 9.01e-105 319
Msa0645450 AT4G27820.2 40.723 415 228 6 111 521 1 401 2.12e-103 317
Msa0645450 AT4G27820.3 40.723 415 228 6 111 521 1 401 2.12e-103 317
Msa0645450 AT1G51490.1 46.910 356 164 8 46 391 17 357 2.74e-103 317
Msa0645450 AT3G62740.2 37.609 460 242 10 9 463 4 423 2.16e-102 315
Msa0645450 AT2G44480.5 48.173 301 155 1 220 520 6 305 2.46e-101 308
Msa0645450 AT1G45191.3 45.820 323 166 4 41 362 26 340 9.98e-100 307
Msa0645450 AT1G45191.5 45.820 323 166 4 41 362 26 340 1.03e-99 307
Msa0645450 AT3G62750.7 42.382 361 172 8 5 364 3 328 1.13e-99 306
Msa0645450 AT3G62750.8 42.382 361 172 8 5 364 3 328 1.13e-99 306
Msa0645450 AT3G62750.3 43.213 361 169 8 5 364 29 354 8.74e-99 306
Msa0645450 AT3G18070.2 45.732 328 169 6 195 519 100 421 3.94e-98 303
Msa0645450 AT3G18070.2 53.968 63 28 1 43 105 30 91 4.36e-14 74.7
Msa0645450 AT3G62750.6 39.518 415 224 8 111 521 1 392 4.43e-98 303
Msa0645450 AT3G62750.5 39.518 415 224 8 111 521 1 392 4.43e-98 303
Msa0645450 AT3G18070.7 45.397 315 163 6 208 519 1 309 1.76e-92 285
Msa0645450 AT1G61810.2 45.215 303 160 3 10 312 4 300 6.13e-89 276
Msa0645450 AT1G60270.2 49.383 243 120 2 113 355 3 242 2.61e-80 253
Msa0645450 AT3G18070.5 43.644 236 129 3 195 428 100 333 3.86e-65 215
Msa0645450 AT3G18070.5 53.968 63 28 1 43 105 30 91 3.19e-14 74.7
Msa0645450 AT3G18070.6 43.049 223 123 3 208 428 1 221 8.95e-60 197
Msa0645450 AT5G16580.1 37.631 287 164 7 189 474 27 299 6.69e-51 176
Msa0645450 AT3G06510.1 25.537 419 239 13 106 518 159 510 1.26e-29 123
Msa0645450 AT3G06510.2 25.342 438 250 13 106 518 159 544 3.00e-29 122

Find 140 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAGCTTCTTCTGCATTTC+AGG 0.143961 4_3:+50701856 Msa0645450:CDS
ATGGAAAAGGACCAAGTATT+TGG 0.197865 4_3:+50702039 Msa0645450:CDS
GACCTTTGCTACAAGGAATT+TGG 0.209271 4_3:+50703331 Msa0645450:CDS
GCATCTCAATATGGTATAAT+AGG 0.209400 4_3:+50703726 Msa0645450:CDS
GTTCTATGGTTTCCCTTTAT+AGG 0.230542 4_3:-50701625 None:intergenic
CGATTCTATAAGTATCTAAA+AGG 0.249841 4_3:-50704898 None:intergenic
ATAATATACATTACTGAAAA+TGG 0.254622 4_3:+50704735 Msa0645450:CDS
CCTCCCTCCTCCTCCTATAA+AGG 0.257504 4_3:+50701612 Msa0645450:five_prime_UTR
GTGCATAAACATCATTAATA+TGG 0.263362 4_3:+50701673 Msa0645450:exon
AAAACAGCAGACCTGATATT+TGG 0.263998 4_3:-50703554 None:intergenic
AAATTGAAGGTAACGTTCTA+TGG 0.276797 4_3:-50701639 None:intergenic
AATTATGGATTCGATTATTT+AGG 0.280439 4_3:-50701536 None:intergenic
AATCATAAAGGATTTGAATA+TGG 0.307459 4_3:+50702806 Msa0645450:CDS
TATAGCTATTCTCCATAAAA+AGG 0.308127 4_3:-50705562 None:intergenic
AAGATCCTCGGATTCTTCTT+TGG 0.311879 4_3:-50704222 None:intergenic
AACAATGGTGGTGGCGATAA+TGG 0.312671 4_3:-50701738 None:intergenic
TAAATAATAAAATAAAATTA+TGG 0.317471 4_3:-50701551 None:intergenic
AATTGCACAGGAGGTGATTC+GGG 0.327589 4_3:+50703463 Msa0645450:CDS
AACCAATGTGCCGAGAGTTT+TGG 0.337227 4_3:-50705222 None:intergenic
TGCATTTATCCGAGAGGATT+AGG 0.340389 4_3:+50704672 Msa0645450:CDS
CGAGGCCTCGACTTCATGTT+TGG 0.348528 4_3:+50703816 Msa0645450:CDS
AGAAGAATCCGAGGATCTTA+AGG 0.358352 4_3:+50704226 Msa0645450:CDS
GATTCTTCGAGTGACAGTTT+TGG 0.360269 4_3:-50704875 None:intergenic
GGACGTTCCTCAAGCCTTAG+AGG 0.360927 4_3:+50703144 Msa0645450:CDS
TCTGGCTTCAGCTTGCGATT+TGG 0.362792 4_3:+50705159 Msa0645450:CDS
TATGTATTTCAGGAAGATAT+TGG 0.369128 4_3:+50702783 Msa0645450:intron
GCTACAAGGAATTTGGTGAT+AGG 0.371977 4_3:+50703338 Msa0645450:CDS
AACAAAGCCTGGCGGGAAAC+AGG 0.373667 4_3:-50701828 None:intergenic
GATAAGAGAGTCAGTTTGTA+TGG 0.374381 4_3:-50704330 None:intergenic
TGAAGCCAGATTCCCATTCA+TGG 0.380755 4_3:-50705146 None:intergenic
CACCGGGTCGATGTTCTGAT+TGG 0.383994 4_3:+50703431 Msa0645450:CDS
AAAATAAGAGATGGAGGTAA+TGG 0.386283 4_3:+50702658 Msa0645450:CDS
GGAAGATATTGGAATCATAA+AGG 0.386661 4_3:+50702794 Msa0645450:intron
AGGATTAGAAAATAGTGATA+TGG 0.390437 4_3:+50705299 Msa0645450:three_prime_UTR
GAATTGCACAGGAGGTGATT+CGG 0.391598 4_3:+50703462 Msa0645450:CDS
GTCAGTTTGTATGGCTGGTC+TGG 0.402360 4_3:-50704321 None:intergenic
TGGAAAAGGACCAAGTATTT+GGG 0.404129 4_3:+50702040 Msa0645450:CDS
CAACCTCATCAACGAACTAT+TGG 0.405447 4_3:+50703003 Msa0645450:CDS
GATAACCATGAATGGGAATC+TGG 0.405714 4_3:+50705141 Msa0645450:CDS
CATCTCAATATGGTATAATA+GGG 0.411825 4_3:+50703727 Msa0645450:CDS
AGGTGCATCAGCAGCATAAG+AGG 0.414938 4_3:-50704285 None:intergenic
TGTAGTAGTTTATTCCTTCA+CGG 0.418155 4_3:-50702979 None:intergenic
CTCCCTCCTCCTCCTATAAA+GGG 0.425999 4_3:+50701613 Msa0645450:five_prime_UTR
TATTCTTGACGTAAAGCAAC+AGG 0.430967 4_3:-50704698 None:intergenic
AAGAGGAAGAGTAATAGTTT+AGG 0.433722 4_3:-50704268 None:intergenic
TGTTCTGTTTAAATGAACTT+AGG 0.434940 4_3:+50705279 Msa0645450:exon
AGGGAAGGTGTGAATGTAAA+GGG 0.435489 4_3:+50705096 Msa0645450:intron
GCGATTTGGACTCGTCTTTG+TGG 0.435971 4_3:+50705173 Msa0645450:CDS
GAAATGCAGGCATCTCAATA+TGG 0.438902 4_3:+50703717 Msa0645450:intron
ATTATGTTCAAGCTGCAATC+AGG 0.440754 4_3:+50704942 Msa0645450:CDS
GATCGGAAAAGTCGCGAAGA+TGG 0.446253 4_3:-50701796 None:intergenic
TAATGATGTTTATGCACAAA+GGG 0.446540 4_3:-50701667 None:intergenic
TTTGCAGAAGGAAAGCTTAG+TGG 0.446993 4_3:+50702947 Msa0645450:intron
ACTATACAAGACCAAATATC+AGG 0.449280 4_3:+50703543 Msa0645450:CDS
ATCAACGAACTATTGGCTAA+TGG 0.455297 4_3:+50703010 Msa0645450:CDS
GGATTACCTTACTCTCTCAT+TGG 0.457583 4_3:+50703748 Msa0645450:CDS
CTTTGGAGAACTTTGGTAGC+CGG 0.461441 4_3:-50704205 None:intergenic
GAAGAATCCGAGGATCTTAA+GGG 0.463005 4_3:+50704227 Msa0645450:CDS
AGAGAGTCAGTTTGTATGGC+TGG 0.463847 4_3:-50704326 None:intergenic
AATCGGACCTGTTTCCCGCC+AGG 0.464039 4_3:+50701821 Msa0645450:CDS
CTACAAGGAATTTGGTGATA+GGG 0.466831 4_3:+50703339 Msa0645450:CDS
ACCGTCATACTCATCCTCTA+AGG 0.470920 4_3:-50703158 None:intergenic
GATTCTTCTTTGGAGAACTT+TGG 0.475194 4_3:-50704212 None:intergenic
TGGTTTCCCTTTATAGGAGG+AGG 0.483745 4_3:-50701619 None:intergenic
TGCGATCTTTGGTTGGAAGC+CGG 0.484554 4_3:+50704186 Msa0645450:CDS
AGCATGAATGCTTATGCATA+TGG 0.485187 4_3:+50703400 Msa0645450:CDS
CATCGTGATGGCAAGCCCCT+TGG 0.493086 4_3:+50704501 Msa0645450:CDS
TTCCGAAAACAAAGCCTGGC+GGG 0.497302 4_3:-50701835 None:intergenic
CAGGGAAGGTGTGAATGTAA+AGG 0.498317 4_3:+50705095 Msa0645450:intron
CTCATACCAATGAGAGAGTA+AGG 0.499326 4_3:-50703754 None:intergenic
CAGCAGCATGAGCAAGAAGT+TGG 0.509755 4_3:-50703513 None:intergenic
TATATGGATCCAATCACAAA+AGG 0.510867 4_3:+50704146 Msa0645450:CDS
TGGTAGTGCGCAGTCAAATA+CGG 0.513115 4_3:-50703493 None:intergenic
CTACGCTGACCTTTGCTACA+AGG 0.513290 4_3:+50703324 Msa0645450:CDS
CATTGTTGGATAACCATGAA+TGG 0.514197 4_3:+50705133 Msa0645450:CDS
CACCAAATTCCTTGTAGCAA+AGG 0.520911 4_3:-50703333 None:intergenic
ATTGTTGGATAACCATGAAT+GGG 0.522990 4_3:+50705134 Msa0645450:CDS
ACAAAGGGAAATGAAATTGA+AGG 0.523224 4_3:-50701652 None:intergenic
TCTGCTTTCGCTTGAGAGGC+CGG 0.524404 4_3:-50703777 None:intergenic
AACATCTGCTTTCGCTTGAG+AGG 0.524538 4_3:-50703781 None:intergenic
CTATGGTTTCCCTTTATAGG+AGG 0.527230 4_3:-50701622 None:intergenic
TGGTTAAATCTGAATTGCAC+AGG 0.528273 4_3:+50703451 Msa0645450:CDS
AACAGGTTTCCTAATCCTCT+CGG 0.530574 4_3:-50704681 None:intergenic
TTGGTGATAGGGTGAAACAT+TGG 0.532663 4_3:+50703350 Msa0645450:CDS
ACTCTCTCATTGGTATGAGC+CGG 0.532738 4_3:+50703758 Msa0645450:CDS
TGGCAAGCCCCTTGGTCCAA+TGG 0.533454 4_3:+50704509 Msa0645450:CDS
TGCATATGGAAAGTTTGCAC+CGG 0.533544 4_3:+50703414 Msa0645450:CDS
GCAGCATTTGAAGATGGAAA+AGG 0.538475 4_3:+50702026 Msa0645450:CDS
GCTGTTCCGAAAACAAAGCC+TGG 0.539523 4_3:-50701839 None:intergenic
GATTCTATAAGTATCTAAAA+GGG 0.541771 4_3:-50704897 None:intergenic
AATATATACCATTGGACCAA+GGG 0.544956 4_3:-50704517 None:intergenic
TTTCCCTTTATAGGAGGAGG+AGG 0.546300 4_3:-50701616 None:intergenic
AAAAGCATGCGATCTTTGGT+TGG 0.548161 4_3:+50704179 Msa0645450:CDS
TCGATGACAGTTCATCGTGA+TGG 0.548253 4_3:+50704489 Msa0645450:intron
GCCTCGACTTCATGTTTGGA+TGG 0.549073 4_3:+50703820 Msa0645450:CDS
GAAGGTGCAGCATTTGAAGA+TGG 0.552430 4_3:+50702020 Msa0645450:CDS
GTGAAAGTATCCCAAATACT+TGG 0.559968 4_3:-50702050 None:intergenic
TAGCCAATAGTTCGTTGATG+AGG 0.565702 4_3:-50703006 None:intergenic
TTTCAGTAATGTATATTATG+GGG 0.565878 4_3:-50704731 None:intergenic
ATATGCAGTCTGCTTCAAGT+TGG 0.568943 4_3:+50704646 Msa0645450:intron
CGAAGATGGAGTAGTGAAGA+AGG 0.570282 4_3:-50701782 None:intergenic
CGACGCATATCATCGGTACA+AGG 0.572001 4_3:+50702693 Msa0645450:CDS
GCATTCATGCTCACACTCCA+TGG 0.576199 4_3:-50703388 None:intergenic
GAAACAGGTCCGATTGAGAT+CGG 0.577440 4_3:-50701813 None:intergenic
CAATGGAAAAGAGTCACATA+TGG 0.580739 4_3:-50703121 None:intergenic
AGCATTGATATGAGAAACAA+TGG 0.584246 4_3:-50701753 None:intergenic
GAAAAGACACCCAAAACTCT+CGG 0.586397 4_3:+50705212 Msa0645450:CDS
TTAATGATGTTTATGCACAA+AGG 0.589128 4_3:-50701668 None:intergenic
GCATATGGAAAGTTTGCACC+GGG 0.592559 4_3:+50703415 Msa0645450:CDS
TACTCATCCTCTAAGGCTTG+AGG 0.594134 4_3:-50703151 None:intergenic
CCTTTATAGGAGGAGGAGGG+AGG 0.597186 4_3:-50701612 None:intergenic
TAGCCGACGACGCATATCAT+CGG 0.598194 4_3:+50702686 Msa0645450:CDS
AAATATATACCATTGGACCA+AGG 0.598574 4_3:-50704518 None:intergenic
ACCCAAAACTCTCGGCACAT+TGG 0.602316 4_3:+50705220 Msa0645450:CDS
TTCCCTTTATAGGAGGAGGA+GGG 0.603110 4_3:-50701615 None:intergenic
GGATTACTTTGAATGAACCA+TGG 0.603853 4_3:+50703371 Msa0645450:CDS
AGGCTTGAGGAACGTCCCAA+TGG 0.604842 4_3:-50703138 None:intergenic
GTTTAGGCCAAGAAAATCGA+AGG 0.606609 4_3:-50704252 None:intergenic
GAAGGACCCCTTAAGATCCT+CGG 0.611639 4_3:-50704234 None:intergenic
GGTGTCACTCACACAATCCG+AGG 0.616673 4_3:-50703190 None:intergenic
GGTCTGGCATCGCGTAAGTG+AGG 0.617392 4_3:-50704305 None:intergenic
GCCTTAGAGGATGAGTATGA+CGG 0.628770 4_3:+50703157 Msa0645450:CDS
AACCAATCAGAACATCGACC+CGG 0.630892 4_3:-50703433 None:intergenic
GCAGAAGGAAAGCTTAGTGG+AGG 0.637778 4_3:+50702950 Msa0645450:intron
ATATATACCATTGGACCAAG+GGG 0.639799 4_3:-50704516 None:intergenic
AGTGGAGGTGTAAACCGTGA+AGG 0.641058 4_3:+50702965 Msa0645450:CDS
ACCATCCAAACATGAAGTCG+AGG 0.641696 4_3:-50703821 None:intergenic
GTTCTCCAAAGAAGAATCCG+AGG 0.647399 4_3:+50704217 Msa0645450:CDS
TGGTTATGCATTTATCCGAG+AGG 0.653174 4_3:+50704666 Msa0645450:CDS
TAGCACTCTAGACCAAGCAA+TGG 0.660595 4_3:-50702844 None:intergenic
GTGTCACTCACACAATCCGA+GGG 0.662250 4_3:-50703189 None:intergenic
TTAAATCTGAATTGCACAGG+AGG 0.663041 4_3:+50703454 Msa0645450:CDS
GATATGAGAAACAATGGTGG+TGG 0.665043 4_3:-50701747 None:intergenic
GTACCGATGATATGCGTCGT+CGG 0.669538 4_3:-50702689 None:intergenic
AAGAATCCGAGGATCTTAAG+GGG 0.674534 4_3:+50704228 Msa0645450:CDS
ATTGCACAGGAGGTGATTCG+GGG 0.685472 4_3:+50703464 Msa0645450:CDS
ACTTTCACTCATGAACACCC+AGG 0.690909 4_3:+50702065 Msa0645450:CDS
GTTCCGAAAACAAAGCCTGG+CGG 0.701608 4_3:-50701836 None:intergenic
GATGTTGATGCTGCACAACG+AGG 0.729333 4_3:+50703798 Msa0645450:CDS
ATTGATATGAGAAACAATGG+TGG 0.754854 4_3:-50701750 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAATAAAATAAAATTA+TGG - chr4_3:50701554-50701573 None:intergenic 0.0%
!!! TTAGATTTTTTTTTTATTCA+AGG + chr4_3:50701583-50701602 Msa0645450:five_prime_UTR 10.0%
!!! TTAAAAATTTTGTAGTATGA+AGG + chr4_3:50702002-50702021 Msa0645450:intron 15.0%
!! ATATTAATATCATGTTTCTT+TGG + chr4_3:50702128-50702147 Msa0645450:intron 15.0%
!! TCTCATATATAATAATGAAA+TGG + chr4_3:50702626-50702645 Msa0645450:intron 15.0%
!! AGCAATATATTATTCAAATT+CGG + chr4_3:50703581-50703600 Msa0645450:intron 15.0%
!! AATGATAAAATATATACCAT+TGG - chr4_3:50704528-50704547 None:intergenic 15.0%
!!! TTTTCAGTAATGTATATTAT+GGG - chr4_3:50704735-50704754 None:intergenic 15.0%
!!! ATTTTCAGTAATGTATATTA+TGG - chr4_3:50704736-50704755 None:intergenic 15.0%
!! ATAATATACATTACTGAAAA+TGG + chr4_3:50704735-50704754 Msa0645450:CDS 15.0%
!! AATAATAGGTAAATGTAAAA+AGG - chr4_3:50705060-50705079 None:intergenic 15.0%
!! AATTATGGATTCGATTATTT+AGG - chr4_3:50701539-50701558 None:intergenic 20.0%
!! GAAGTAATTAAAAATAACCT+GGG - chr4_3:50702085-50702104 None:intergenic 20.0%
!! AGAAGTAATTAAAAATAACC+TGG - chr4_3:50702086-50702105 None:intergenic 20.0%
!! TAAATCGTTAGATTAAGATA+GGG + chr4_3:50702231-50702250 Msa0645450:intron 20.0%
!! AATCATAAAGGATTTGAATA+TGG + chr4_3:50702806-50702825 Msa0645450:CDS 20.0%
!! TTACAAAAATATGACTTACT+TGG - chr4_3:50702870-50702889 None:intergenic 20.0%
!! ACTATATCACAAATGTATAA+GGG - chr4_3:50702901-50702920 None:intergenic 20.0%
!! TACTATATCACAAATGTATA+AGG - chr4_3:50702902-50702921 None:intergenic 20.0%
!! ATAATAATTGAGAATAATGC+AGG + chr4_3:50703091-50703110 Msa0645450:intron 20.0%
!! CTATTTATCTATGACTATTT+AGG + chr4_3:50703229-50703248 Msa0645450:intron 20.0%
!!! GCATTTCTCAAAATTTTATT+TGG - chr4_3:50703704-50703723 None:intergenic 20.0%
!!! AATAAAATTTTGAGAAATGC+AGG + chr4_3:50703704-50703723 Msa0645450:intron 20.0%
!!! ATGTAAACTTCATTTGATTT+TGG + chr4_3:50703970-50703989 Msa0645450:intron 20.0%
!! TTTCAGTAATGTATATTATG+GGG - chr4_3:50704734-50704753 None:intergenic 20.0%
!! GATTCTATAAGTATCTAAAA+GGG - chr4_3:50704900-50704919 None:intergenic 20.0%
!! GAAAAAAAATTGCCAATAAA+AGG + chr4_3:50705355-50705374 Msa0645450:three_prime_UTR 20.0%
! TAATGATGTTTATGCACAAA+GGG - chr4_3:50701670-50701689 None:intergenic 25.0%
! TTAATGATGTTTATGCACAA+AGG - chr4_3:50701671-50701690 None:intergenic 25.0%
! GTGCATAAACATCATTAATA+TGG + chr4_3:50701673-50701692 Msa0645450:exon 25.0%
!! ATCATGTTTCTTTGGTAATA+TGG + chr4_3:50702136-50702155 Msa0645450:intron 25.0%
!! TCATGTTTCTTTGGTAATAT+GGG + chr4_3:50702137-50702156 Msa0645450:intron 25.0%
! GTAAATCGTTAGATTAAGAT+AGG + chr4_3:50702230-50702249 Msa0645450:intron 25.0%
! AATTCATATCCGTTCATTTA+GGG + chr4_3:50702307-50702326 Msa0645450:intron 25.0%
! AAATGTTGCACTAAATTGAA+TGG - chr4_3:50702390-50702409 None:intergenic 25.0%
!! CTTTTCCTTAATGATGTATT+TGG + chr4_3:50702472-50702491 Msa0645450:intron 25.0%
! TATGTATTTCAGGAAGATAT+TGG + chr4_3:50702783-50702802 Msa0645450:intron 25.0%
!!! TACTTATTTTCATATCCATG+TGG + chr4_3:50703055-50703074 Msa0645450:intron 25.0%
! AATAGCTCAAGATTGAAAAT+TGG - chr4_3:50703214-50703233 None:intergenic 25.0%
!!! GCAATTGTTATTTGAATTTC+AGG - chr4_3:50703623-50703642 None:intergenic 25.0%
! CATCTCAATATGGTATAATA+GGG + chr4_3:50703727-50703746 Msa0645450:CDS 25.0%
! ATGATATGATCATGATAATC+TGG + chr4_3:50703937-50703956 Msa0645450:intron 25.0%
! ATTACTTTACTTTGCACAAA+TGG - chr4_3:50704063-50704082 None:intergenic 25.0%
! TTTGTGCAAAGTAAAGTAAT+AGG + chr4_3:50704063-50704082 Msa0645450:intron 25.0%
! CGATTCTATAAGTATCTAAA+AGG - chr4_3:50704901-50704920 None:intergenic 25.0%
! TAGGTAAATGTAAAAAGGAA+GGG - chr4_3:50705055-50705074 None:intergenic 25.0%
! ATAGGTAAATGTAAAAAGGA+AGG - chr4_3:50705056-50705075 None:intergenic 25.0%
! TATGCATAAAGGTCAATAAT+AGG - chr4_3:50705074-50705093 None:intergenic 25.0%
!! TTTAAACAGAACACTTTTCT+TGG - chr4_3:50705272-50705291 None:intergenic 25.0%
! TGTTCTGTTTAAATGAACTT+AGG + chr4_3:50705279-50705298 Msa0645450:exon 25.0%
! AGGATTAGAAAATAGTGATA+TGG + chr4_3:50705299-50705318 Msa0645450:three_prime_UTR 25.0%
!!! CTTTTCTAGAAACCTTTTAT+TGG - chr4_3:50705370-50705389 None:intergenic 25.0%
!! CTATTTGTGTAACCTTTTTA+TGG + chr4_3:50705550-50705569 Msa0645450:three_prime_UTR 25.0%
! TATAGCTATTCTCCATAAAA+AGG - chr4_3:50705565-50705584 None:intergenic 25.0%
AAATTGAAGGTAACGTTCTA+TGG - chr4_3:50701642-50701661 None:intergenic 30.0%
ACAAAGGGAAATGAAATTGA+AGG - chr4_3:50701655-50701674 None:intergenic 30.0%
ATTGATATGAGAAACAATGG+TGG - chr4_3:50701753-50701772 None:intergenic 30.0%
!! AGCATTGATATGAGAAACAA+TGG - chr4_3:50701756-50701775 None:intergenic 30.0%
ATATTATTTGGACATGTTGC+AGG - chr4_3:50702169-50702188 None:intergenic 30.0%
GAATTCATATCCGTTCATTT+AGG + chr4_3:50702306-50702325 Msa0645450:intron 30.0%
TCATTTATGCCCTAAATGAA+CGG - chr4_3:50702319-50702338 None:intergenic 30.0%
! TCATTTAGGGCATAAATGAA+CGG + chr4_3:50702320-50702339 Msa0645450:intron 30.0%
TTGCACCAAATACATCATTA+AGG - chr4_3:50702480-50702499 None:intergenic 30.0%
CTTGCAGAAAAAATAAGAGA+TGG + chr4_3:50702649-50702668 Msa0645450:intron 30.0%
AAAATAAGAGATGGAGGTAA+TGG + chr4_3:50702658-50702677 Msa0645450:CDS 30.0%
TAGATCACTCTATGTATTTC+AGG + chr4_3:50702773-50702792 Msa0645450:intron 30.0%
GGAAGATATTGGAATCATAA+AGG + chr4_3:50702794-50702813 Msa0645450:intron 30.0%
! CTTATAGATTTTCCATTGCT+TGG + chr4_3:50702832-50702851 Msa0645450:CDS 30.0%
ATATCACAAATGTATAAGGG+AGG - chr4_3:50702898-50702917 None:intergenic 30.0%
TCCTATTAACTATTTGCAGA+AGG + chr4_3:50702935-50702954 Msa0645450:intron 30.0%
TCCTTCTGCAAATAGTTAAT+AGG - chr4_3:50702939-50702958 None:intergenic 30.0%
TGTAGTAGTTTATTCCTTCA+CGG - chr4_3:50702982-50703001 None:intergenic 30.0%
ATTTATGATTGACAACCACA+TGG - chr4_3:50703073-50703092 None:intergenic 30.0%
! ATATGTGACTCTTTTCCATT+GGG + chr4_3:50703123-50703142 Msa0645450:CDS 30.0%
! ATCTTGCAGAGATGATTTTA+GGG + chr4_3:50703300-50703319 Msa0645450:intron 30.0%
ACTATACAAGACCAAATATC+AGG + chr4_3:50703543-50703562 Msa0645450:CDS 30.0%
GCATCTCAATATGGTATAAT+AGG + chr4_3:50703726-50703745 Msa0645450:CDS 30.0%
TATATGGATCCAATCACAAA+AGG + chr4_3:50704146-50704165 Msa0645450:CDS 30.0%
AAGAGGAAGAGTAATAGTTT+AGG - chr4_3:50704271-50704290 None:intergenic 30.0%
AATATATACCATTGGACCAA+GGG - chr4_3:50704520-50704539 None:intergenic 30.0%
AAATATATACCATTGGACCA+AGG - chr4_3:50704521-50704540 None:intergenic 30.0%
!! GCTTCTAGTTATTGATTTGT+AGG + chr4_3:50704995-50705014 Msa0645450:intron 30.0%
TATTGACCTTTATGCATAAC+AGG + chr4_3:50705076-50705095 Msa0645450:intron 30.0%
ATTGACCTTTATGCATAACA+GGG + chr4_3:50705077-50705096 Msa0645450:intron 30.0%
! ATGTAAAGGGATATTTTGCA+TGG + chr4_3:50705109-50705128 Msa0645450:CDS 30.0%
! ATATTTTGCATGGTCATTGT+TGG + chr4_3:50705119-50705138 Msa0645450:CDS 30.0%
ATTGTTGGATAACCATGAAT+GGG + chr4_3:50705134-50705153 Msa0645450:CDS 30.0%
!!! TTTCTTGGCAAGATTTTTTG+AGG - chr4_3:50705257-50705276 None:intergenic 30.0%
GTTCTATGGTTTCCCTTTAT+AGG - chr4_3:50701628-50701647 None:intergenic 35.0%
ATGGAAAAGGACCAAGTATT+TGG + chr4_3:50702039-50702058 Msa0645450:CDS 35.0%
TGGAAAAGGACCAAGTATTT+GGG + chr4_3:50702040-50702059 Msa0645450:CDS 35.0%
!! GTGAAAGTATCCCAAATACT+TGG - chr4_3:50702053-50702072 None:intergenic 35.0%
TTAAACTTAGTGCAACTTGC+TGG + chr4_3:50702449-50702468 Msa0645450:intron 35.0%
AGCAAAAGCTTATGCTATGT+TGG - chr4_3:50702558-50702577 None:intergenic 35.0%
TCCATTCATGCTATGTAACT+AGG - chr4_3:50702604-50702623 None:intergenic 35.0%
GCAGAAAAAATAAGAGATGG+AGG + chr4_3:50702652-50702671 Msa0645450:intron 35.0%
! ATCAACGAACTATTGGCTAA+TGG + chr4_3:50703010-50703029 Msa0645450:CDS 35.0%
CAATGGAAAAGAGTCACATA+TGG - chr4_3:50703124-50703143 None:intergenic 35.0%
! CATATGTGACTCTTTTCCAT+TGG + chr4_3:50703122-50703141 Msa0645450:CDS 35.0%
! GATCTTGCAGAGATGATTTT+AGG + chr4_3:50703299-50703318 Msa0645450:intron 35.0%
! CTACAAGGAATTTGGTGATA+GGG + chr4_3:50703339-50703358 Msa0645450:CDS 35.0%
GGATTACTTTGAATGAACCA+TGG + chr4_3:50703371-50703390 Msa0645450:CDS 35.0%
AGCATGAATGCTTATGCATA+TGG + chr4_3:50703400-50703419 Msa0645450:CDS 35.0%
TGGTTAAATCTGAATTGCAC+AGG + chr4_3:50703451-50703470 Msa0645450:CDS 35.0%
TTAAATCTGAATTGCACAGG+AGG + chr4_3:50703454-50703473 Msa0645450:CDS 35.0%
AAAACAGCAGACCTGATATT+TGG - chr4_3:50703557-50703576 None:intergenic 35.0%
TATTCGTAGCTCAACACTAA+CGG + chr4_3:50703860-50703879 Msa0645450:intron 35.0%
! GCATTTTGCATTTTTCCTGT+AGG + chr4_3:50704123-50704142 Msa0645450:intron 35.0%
! GCATTTTTCCTGTAGGTATA+TGG + chr4_3:50704130-50704149 Msa0645450:intron 35.0%
! GGATAGTTTCCTTTTGTGAT+TGG - chr4_3:50704158-50704177 None:intergenic 35.0%
! GATTCTTCTTTGGAGAACTT+TGG - chr4_3:50704215-50704234 None:intergenic 35.0%
GATAAGAGAGTCAGTTTGTA+TGG - chr4_3:50704333-50704352 None:intergenic 35.0%
ATATATACCATTGGACCAAG+GGG - chr4_3:50704519-50704538 None:intergenic 35.0%
TATTCTTGACGTAAAGCAAC+AGG - chr4_3:50704701-50704720 None:intergenic 35.0%
GTGTGACAAAATCCATTGAT+AGG + chr4_3:50704833-50704852 Msa0645450:intron 35.0%
ATTATGTTCAAGCTGCAATC+AGG + chr4_3:50704942-50704961 Msa0645450:CDS 35.0%
ATCAATAACTAGAAGCCTAG+AGG - chr4_3:50704991-50705010 None:intergenic 35.0%
CATTGTTGGATAACCATGAA+TGG + chr4_3:50705133-50705152 Msa0645450:CDS 35.0%
CTATGGTTTCCCTTTATAGG+AGG - chr4_3:50701625-50701644 None:intergenic 40.0%
GATATGAGAAACAATGGTGG+TGG - chr4_3:50701750-50701769 None:intergenic 40.0%
AACAGCTTCTTCTGCATTTC+AGG + chr4_3:50701856-50701875 Msa0645450:CDS 40.0%
GCAGCATTTGAAGATGGAAA+AGG + chr4_3:50702026-50702045 Msa0645450:CDS 40.0%
! GCTTTATGATGCAAGTTGGA+CGG - chr4_3:50702276-50702295 None:intergenic 40.0%
!! GTTGGCTTTATGATGCAAGT+TGG - chr4_3:50702280-50702299 None:intergenic 40.0%
GGATATGAATTCGCTACAGT+TGG - chr4_3:50702298-50702317 None:intergenic 40.0%
AGCTCCTATCTTTATGCTTG+TGG + chr4_3:50702351-50702370 Msa0645450:intron 40.0%
GCTCCTATCTTTATGCTTGT+GGG + chr4_3:50702352-50702371 Msa0645450:intron 40.0%
GATCCCACAAGCATAAAGAT+AGG - chr4_3:50702358-50702377 None:intergenic 40.0%
TAAGTTGCTTCTGAATGCCT+TGG + chr4_3:50702528-50702547 Msa0645450:intron 40.0%
TATGCTATGTTGGATCTCCA+AGG - chr4_3:50702548-50702567 None:intergenic 40.0%
GCCTAGTTACATAGCATGAA+TGG + chr4_3:50702600-50702619 Msa0645450:intron 40.0%
!! CAAGGTATGTAGTTTTGCAG+AGG + chr4_3:50702711-50702730 Msa0645450:intron 40.0%
TTTGCAGAAGGAAAGCTTAG+TGG + chr4_3:50702947-50702966 Msa0645450:intron 40.0%
CAACCTCATCAACGAACTAT+TGG + chr4_3:50703003-50703022 Msa0645450:CDS 40.0%
TAGCCAATAGTTCGTTGATG+AGG - chr4_3:50703009-50703028 None:intergenic 40.0%
!!! ATGACGGTTTTTTAAGCCCT+CGG + chr4_3:50703173-50703192 Msa0645450:CDS 40.0%
AAATCATCTCTGCAAGATCG+CGG - chr4_3:50703298-50703317 None:intergenic 40.0%
GACCTTTGCTACAAGGAATT+TGG + chr4_3:50703331-50703350 Msa0645450:CDS 40.0%
CACCAAATTCCTTGTAGCAA+AGG - chr4_3:50703336-50703355 None:intergenic 40.0%
! GCTACAAGGAATTTGGTGAT+AGG + chr4_3:50703338-50703357 Msa0645450:CDS 40.0%
! TTGGTGATAGGGTGAAACAT+TGG + chr4_3:50703350-50703369 Msa0645450:CDS 40.0%
TGCATATGGAAAGTTTGCAC+CGG + chr4_3:50703414-50703433 Msa0645450:CDS 40.0%
GAAATGCAGGCATCTCAATA+TGG + chr4_3:50703717-50703736 Msa0645450:intron 40.0%
GGATTACCTTACTCTCTCAT+TGG + chr4_3:50703748-50703767 Msa0645450:CDS 40.0%
CTCATACCAATGAGAGAGTA+AGG - chr4_3:50703757-50703776 None:intergenic 40.0%
GATTGGATCCATATACCTAC+AGG - chr4_3:50704141-50704160 None:intergenic 40.0%
! TCCAAAAAGCATGCGATCTT+TGG + chr4_3:50704175-50704194 Msa0645450:CDS 40.0%
! ACCAAAGATCGCATGCTTTT+TGG - chr4_3:50704179-50704198 None:intergenic 40.0%
! AAAAGCATGCGATCTTTGGT+TGG + chr4_3:50704179-50704198 Msa0645450:CDS 40.0%
AAGATCCTCGGATTCTTCTT+TGG - chr4_3:50704225-50704244 None:intergenic 40.0%
AGAAGAATCCGAGGATCTTA+AGG + chr4_3:50704226-50704245 Msa0645450:CDS 40.0%
GAAGAATCCGAGGATCTTAA+GGG + chr4_3:50704227-50704246 Msa0645450:CDS 40.0%
AAGAATCCGAGGATCTTAAG+GGG + chr4_3:50704228-50704247 Msa0645450:CDS 40.0%
GTTTAGGCCAAGAAAATCGA+AGG - chr4_3:50704255-50704274 None:intergenic 40.0%
ATATGCAGTCTGCTTCAAGT+TGG + chr4_3:50704646-50704665 Msa0645450:intron 40.0%
TGGTTATGCATTTATCCGAG+AGG + chr4_3:50704666-50704685 Msa0645450:CDS 40.0%
TGCATTTATCCGAGAGGATT+AGG + chr4_3:50704672-50704691 Msa0645450:CDS 40.0%
AACAGGTTTCCTAATCCTCT+CGG - chr4_3:50704684-50704703 None:intergenic 40.0%
! GATTCTTCGAGTGACAGTTT+TGG - chr4_3:50704878-50704897 None:intergenic 40.0%
ACCTTTATGCATAACAGGGA+AGG + chr4_3:50705081-50705100 Msa0645450:intron 40.0%
ACCTTCCCTGTTATGCATAA+AGG - chr4_3:50705085-50705104 None:intergenic 40.0%
! AGGGAAGGTGTGAATGTAAA+GGG + chr4_3:50705096-50705115 Msa0645450:intron 40.0%
GATAACCATGAATGGGAATC+TGG + chr4_3:50705141-50705160 Msa0645450:CDS 40.0%
GAAAAGACACCCAAAACTCT+CGG + chr4_3:50705212-50705231 Msa0645450:CDS 40.0%
TTCCCTTTATAGGAGGAGGA+GGG - chr4_3:50701618-50701637 None:intergenic 45.0%
TTTCCCTTTATAGGAGGAGG+AGG - chr4_3:50701619-50701638 None:intergenic 45.0%
TGGTTTCCCTTTATAGGAGG+AGG - chr4_3:50701622-50701641 None:intergenic 45.0%
!! AACAATGGTGGTGGCGATAA+TGG - chr4_3:50701741-50701760 None:intergenic 45.0%
CGAAGATGGAGTAGTGAAGA+AGG - chr4_3:50701785-50701804 None:intergenic 45.0%
! GCGACTTTTCCGATCTCAAT+CGG + chr4_3:50701804-50701823 Msa0645450:CDS 45.0%
GAAACAGGTCCGATTGAGAT+CGG - chr4_3:50701816-50701835 None:intergenic 45.0%
! GAAGGTGCAGCATTTGAAGA+TGG + chr4_3:50702020-50702039 Msa0645450:CDS 45.0%
ACTTTCACTCATGAACACCC+AGG + chr4_3:50702065-50702084 Msa0645450:CDS 45.0%
GGCATAAATGAACGGACATG+TGG + chr4_3:50702328-50702347 Msa0645450:intron 45.0%
TAGCACTCTAGACCAAGCAA+TGG - chr4_3:50702847-50702866 None:intergenic 45.0%
!! TACTCATCCTCTAAGGCTTG+AGG - chr4_3:50703154-50703173 None:intergenic 45.0%
GCCTTAGAGGATGAGTATGA+CGG + chr4_3:50703157-50703176 Msa0645450:CDS 45.0%
ACCGTCATACTCATCCTCTA+AGG - chr4_3:50703161-50703180 None:intergenic 45.0%
GCATATGGAAAGTTTGCACC+GGG + chr4_3:50703415-50703434 Msa0645450:CDS 45.0%
AACCAATCAGAACATCGACC+CGG - chr4_3:50703436-50703455 None:intergenic 45.0%
GAATTGCACAGGAGGTGATT+CGG + chr4_3:50703462-50703481 Msa0645450:CDS 45.0%
AATTGCACAGGAGGTGATTC+GGG + chr4_3:50703463-50703482 Msa0645450:CDS 45.0%
! TGGTAGTGCGCAGTCAAATA+CGG - chr4_3:50703496-50703515 None:intergenic 45.0%
ACTCTCTCATTGGTATGAGC+CGG + chr4_3:50703758-50703777 Msa0645450:CDS 45.0%
AACATCTGCTTTCGCTTGAG+AGG - chr4_3:50703784-50703803 None:intergenic 45.0%
ACCATCCAAACATGAAGTCG+AGG - chr4_3:50703824-50703843 None:intergenic 45.0%
! CTTTGGAGAACTTTGGTAGC+CGG - chr4_3:50704208-50704227 None:intergenic 45.0%
GTTCTCCAAAGAAGAATCCG+AGG + chr4_3:50704217-50704236 Msa0645450:CDS 45.0%
! AAGGGGTCCTTCGATTTTCT+TGG + chr4_3:50704245-50704264 Msa0645450:CDS 45.0%
AGAGAGTCAGTTTGTATGGC+TGG - chr4_3:50704329-50704348 None:intergenic 45.0%
TCGATGACAGTTCATCGTGA+TGG + chr4_3:50704489-50704508 Msa0645450:intron 45.0%
AACTCATCGCGACCTATCAA+TGG - chr4_3:50704848-50704867 None:intergenic 45.0%
GCTTTATCTGAAGAGCCTCT+AGG + chr4_3:50704973-50704992 Msa0645450:intron 45.0%
! CAGGGAAGGTGTGAATGTAA+AGG + chr4_3:50705095-50705114 Msa0645450:intron 45.0%
TGAAGCCAGATTCCCATTCA+TGG - chr4_3:50705149-50705168 None:intergenic 45.0%
! ACCAATGTGCCGAGAGTTTT+GGG - chr4_3:50705224-50705243 None:intergenic 45.0%
! AACCAATGTGCCGAGAGTTT+TGG - chr4_3:50705225-50705244 None:intergenic 45.0%
!! AATAACATATTAATATTATT+TGG - chr4_3:50702181-50702200 None:intergenic 5.0%
!!! TTTTTTTTTTTTTTTTGAAA+AGG - chr4_3:50702414-50702433 None:intergenic 5.0%
!!! ATATTTTAGAAATAATATTT+TGG - chr4_3:50704603-50704622 None:intergenic 5.0%
!!! TTTAGTTTTAATTTTTTTAA+TGG + chr4_3:50705411-50705430 Msa0645450:three_prime_UTR 5.0%
CTCCCTCCTCCTCCTATAAA+GGG + chr4_3:50701613-50701632 Msa0645450:five_prime_UTR 50.0%
GATCGGAAAAGTCGCGAAGA+TGG - chr4_3:50701799-50701818 None:intergenic 50.0%
!!! TTCCCGCCAGGCTTTGTTTT+CGG + chr4_3:50701833-50701852 Msa0645450:CDS 50.0%
TTCCGAAAACAAAGCCTGGC+GGG - chr4_3:50701838-50701857 None:intergenic 50.0%
GTTCCGAAAACAAAGCCTGG+CGG - chr4_3:50701839-50701858 None:intergenic 50.0%
GCTGTTCCGAAAACAAAGCC+TGG - chr4_3:50701842-50701861 None:intergenic 50.0%
TAGCCGACGACGCATATCAT+CGG + chr4_3:50702686-50702705 Msa0645450:CDS 50.0%
! GTACCGATGATATGCGTCGT+CGG - chr4_3:50702692-50702711 None:intergenic 50.0%
CGACGCATATCATCGGTACA+AGG + chr4_3:50702693-50702712 Msa0645450:CDS 50.0%
GCAGAAGGAAAGCTTAGTGG+AGG + chr4_3:50702950-50702969 Msa0645450:intron 50.0%
! AGTGGAGGTGTAAACCGTGA+AGG + chr4_3:50702965-50702984 Msa0645450:CDS 50.0%
! GTGTCACTCACACAATCCGA+GGG - chr4_3:50703192-50703211 None:intergenic 50.0%
CTACGCTGACCTTTGCTACA+AGG + chr4_3:50703324-50703343 Msa0645450:CDS 50.0%
GCATTCATGCTCACACTCCA+TGG - chr4_3:50703391-50703410 None:intergenic 50.0%
ATTGCACAGGAGGTGATTCG+GGG + chr4_3:50703464-50703483 Msa0645450:CDS 50.0%
CAGCAGCATGAGCAAGAAGT+TGG - chr4_3:50703516-50703535 None:intergenic 50.0%
GATGTTGATGCTGCACAACG+AGG + chr4_3:50703798-50703817 Msa0645450:CDS 50.0%
GCCTCGACTTCATGTTTGGA+TGG + chr4_3:50703820-50703839 Msa0645450:CDS 50.0%
TGCGATCTTTGGTTGGAAGC+CGG + chr4_3:50704186-50704205 Msa0645450:CDS 50.0%
GAAGGACCCCTTAAGATCCT+CGG - chr4_3:50704237-50704256 None:intergenic 50.0%
AGGTGCATCAGCAGCATAAG+AGG - chr4_3:50704288-50704307 None:intergenic 50.0%
! GTCAGTTTGTATGGCTGGTC+TGG - chr4_3:50704324-50704343 None:intergenic 50.0%
! TCTGGCTTCAGCTTGCGATT+TGG + chr4_3:50705159-50705178 Msa0645450:CDS 50.0%
! GCGATTTGGACTCGTCTTTG+TGG + chr4_3:50705173-50705192 Msa0645450:CDS 50.0%
ACCCAAAACTCTCGGCACAT+TGG + chr4_3:50705220-50705239 Msa0645450:CDS 50.0%
CCTTTATAGGAGGAGGAGGG+AGG - chr4_3:50701615-50701634 None:intergenic 55.0%
CCTCCCTCCTCCTCCTATAA+AGG + chr4_3:50701612-50701631 Msa0645450:five_prime_UTR 55.0%
AACAAAGCCTGGCGGGAAAC+AGG - chr4_3:50701831-50701850 None:intergenic 55.0%
AGGCTTGAGGAACGTCCCAA+TGG - chr4_3:50703141-50703160 None:intergenic 55.0%
GGACGTTCCTCAAGCCTTAG+AGG + chr4_3:50703144-50703163 Msa0645450:CDS 55.0%
!! GGTGTCACTCACACAATCCG+AGG - chr4_3:50703193-50703212 None:intergenic 55.0%
CACCGGGTCGATGTTCTGAT+TGG + chr4_3:50703431-50703450 Msa0645450:CDS 55.0%
TCTGCTTTCGCTTGAGAGGC+CGG - chr4_3:50703780-50703799 None:intergenic 55.0%
CGAGGCCTCGACTTCATGTT+TGG + chr4_3:50703816-50703835 Msa0645450:CDS 55.0%
AATCGGACCTGTTTCCCGCC+AGG + chr4_3:50701821-50701840 Msa0645450:CDS 60.0%
GGTCTGGCATCGCGTAAGTG+AGG - chr4_3:50704308-50704327 None:intergenic 60.0%
CATCGTGATGGCAAGCCCCT+TGG + chr4_3:50704501-50704520 Msa0645450:CDS 60.0%
TGGCAAGCCCCTTGGTCCAA+TGG + chr4_3:50704509-50704528 Msa0645450:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 50701517 50705584 50701517 ID=Msa0645450;Name=Msa0645450
chr4_3 mRNA 50701517 50705584 50701517 ID=Msa0645450-mRNA-1;Parent=Msa0645450;Name=Msa0645450-mRNA-1;_AED=0.16;_eAED=0.16;_QI=175|1|1|1|1|1|13|294|527
chr4_3 exon 50701517 50701877 50701517 ID=Msa0645450-mRNA-1:exon:12088;Parent=Msa0645450-mRNA-1
chr4_3 exon 50702017 50702086 50702017 ID=Msa0645450-mRNA-1:exon:12089;Parent=Msa0645450-mRNA-1
chr4_3 exon 50702656 50702714 50702656 ID=Msa0645450-mRNA-1:exon:12090;Parent=Msa0645450-mRNA-1
chr4_3 exon 50702795 50702870 50702795 ID=Msa0645450-mRNA-1:exon:12091;Parent=Msa0645450-mRNA-1
chr4_3 exon 50702954 50703031 50702954 ID=Msa0645450-mRNA-1:exon:12092;Parent=Msa0645450-mRNA-1
chr4_3 exon 50703113 50703200 50703113 ID=Msa0645450-mRNA-1:exon:12093;Parent=Msa0645450-mRNA-1
chr4_3 exon 50703309 50703564 50703309 ID=Msa0645450-mRNA-1:exon:12094;Parent=Msa0645450-mRNA-1
chr4_3 exon 50703726 50703841 50703726 ID=Msa0645450-mRNA-1:exon:12095;Parent=Msa0645450-mRNA-1
chr4_3 exon 50704145 50704362 50704145 ID=Msa0645450-mRNA-1:exon:12096;Parent=Msa0645450-mRNA-1
chr4_3 exon 50704499 50704530 50704499 ID=Msa0645450-mRNA-1:exon:12097;Parent=Msa0645450-mRNA-1
chr4_3 exon 50704654 50704756 50704654 ID=Msa0645450-mRNA-1:exon:12098;Parent=Msa0645450-mRNA-1
chr4_3 exon 50704855 50704963 50704855 ID=Msa0645450-mRNA-1:exon:12099;Parent=Msa0645450-mRNA-1
chr4_3 exon 50705098 50705584 50705098 ID=Msa0645450-mRNA-1:exon:12100;Parent=Msa0645450-mRNA-1
chr4_3 five_prime_UTR 50701517 50701691 50701517 ID=Msa0645450-mRNA-1:five_prime_utr;Parent=Msa0645450-mRNA-1
chr4_3 CDS 50701692 50701877 50701692 ID=Msa0645450-mRNA-1:cds;Parent=Msa0645450-mRNA-1
chr4_3 CDS 50702017 50702086 50702017 ID=Msa0645450-mRNA-1:cds;Parent=Msa0645450-mRNA-1
chr4_3 CDS 50702656 50702714 50702656 ID=Msa0645450-mRNA-1:cds;Parent=Msa0645450-mRNA-1
chr4_3 CDS 50702795 50702870 50702795 ID=Msa0645450-mRNA-1:cds;Parent=Msa0645450-mRNA-1
chr4_3 CDS 50702954 50703031 50702954 ID=Msa0645450-mRNA-1:cds;Parent=Msa0645450-mRNA-1
chr4_3 CDS 50703113 50703200 50703113 ID=Msa0645450-mRNA-1:cds;Parent=Msa0645450-mRNA-1
chr4_3 CDS 50703309 50703564 50703309 ID=Msa0645450-mRNA-1:cds;Parent=Msa0645450-mRNA-1
chr4_3 CDS 50703726 50703841 50703726 ID=Msa0645450-mRNA-1:cds;Parent=Msa0645450-mRNA-1
chr4_3 CDS 50704145 50704362 50704145 ID=Msa0645450-mRNA-1:cds;Parent=Msa0645450-mRNA-1
chr4_3 CDS 50704499 50704530 50704499 ID=Msa0645450-mRNA-1:cds;Parent=Msa0645450-mRNA-1
chr4_3 CDS 50704654 50704756 50704654 ID=Msa0645450-mRNA-1:cds;Parent=Msa0645450-mRNA-1
chr4_3 CDS 50704855 50704963 50704855 ID=Msa0645450-mRNA-1:cds;Parent=Msa0645450-mRNA-1
chr4_3 CDS 50705098 50705290 50705098 ID=Msa0645450-mRNA-1:cds;Parent=Msa0645450-mRNA-1
chr4_3 three_prime_UTR 50705291 50705584 50705291 ID=Msa0645450-mRNA-1:three_prime_utr;Parent=Msa0645450-mRNA-1
Gene Sequence

>Msa0645450

ATGGCAGCATTTCATTTCAACGTGTTTTTGTTACTCTTCATTTTTTCCATTATCGCCACCACCATTGTTTCTCATATCAATGCTTTTGAACCTTCTTCACTACTCCATCTTCGCGACTTTTCCGATCTCAATCGGACCTGTTTCCCGCCAGGCTTTGTTTTCGGAACAGCTTCTTCTGCATTTCAGTATGAAGGTGCAGCATTTGAAGATGGAAAAGGACCAAGTATTTGGGATACTTTCACTCATGAACACCCAGAAAAAATAAGAGATGGAGGTAATGGAGATGTAGCCGACGACGCATATCATCGGTACAAGGAAGATATTGGAATCATAAAGGATTTGAATATGGATGCTTATAGATTTTCCATTGCTTGGTCTAGAGTGCTACCAAAAGGAAAGCTTAGTGGAGGTGTAAACCGTGAAGGAATAAACTACTACAACAACCTCATCAACGAACTATTGGCTAATGGTTTGCAGCCATATGTGACTCTTTTCCATTGGGACGTTCCTCAAGCCTTAGAGGATGAGTATGACGGTTTTTTAAGCCCTCGGATTGTAGATGATTTTAGGGACTACGCTGACCTTTGCTACAAGGAATTTGGTGATAGGGTGAAACATTGGATTACTTTGAATGAACCATGGAGTGTGAGCATGAATGCTTATGCATATGGAAAGTTTGCACCGGGTCGATGTTCTGATTGGTTAAATCTGAATTGCACAGGAGGTGATTCGGGGACAGAACCGTATTTGACTGCGCACTACCAACTTCTTGCTCATGCTGCTGCTACAAAACTATACAAGACCAAATATCAGGCATCTCAATATGGTATAATAGGGATTACCTTACTCTCTCATTGGTATGAGCCGGCCTCTCAAGCGAAAGCAGATGTTGATGCTGCACAACGAGGCCTCGACTTCATGTTTGGATGGTATATGGATCCAATCACAAAAGGAAACTATCCAAAAAGCATGCGATCTTTGGTTGGAAGCCGGCTACCAAAGTTCTCCAAAGAAGAATCCGAGGATCTTAAGGGGTCCTTCGATTTTCTTGGCCTAAACTATTACTCTTCCTCTTATGCTGCTGATGCACCTCACTTACGCGATGCCAGACCAGCCATACAAACTGACTCTCTTATCAATGCTACATTTCATCGTGATGGCAAGCCCCTTGGTCCAATGTCTGCTTCAAGTTGGTTATGCATTTATCCGAGAGGATTAGGAAACCTGTTGCTTTACGTCAAGAATAAGTACGACAACCCCATAATATACATTACTGAAAATGGTCGCGATGAGTTCAACGATCCAAAACTGTCACTCGAAGAATCCCTTTTAGATACTTATAGAATCGATTACTTTTATCGTCATCTTTATTATGTTCAAGCTGCAATCAGGGAAGGTGTGAATGTAAAGGGATATTTTGCATGGTCATTGTTGGATAACCATGAATGGGAATCTGGCTTCAGCTTGCGATTTGGACTCGTCTTTGTGGATTATAATGACGACTTGAAAAGACACCCAAAACTCTCGGCACATTGGTTCAAGAATTTCCTCAAAAAATCTTGCCAAGAAAAGTGTTCTGTTTAA
Protein sequence

>Msa0645450

MAAFHFNVFLLLFIFSIIATTIVSHINAFEPSSLLHLRDFSDLNRTCFPPGFVFGTASSAFQYEGAAFEDGKGPSIWDTFTHEHPEKIRDGGNGDVADDAYHRYKEDIGIIKDLNMDAYRFSIAWSRVLPKGKLSGGVNREGINYYNNLINELLANGLQPYVTLFHWDVPQALEDEYDGFLSPRIVDDFRDYADLCYKEFGDRVKHWITLNEPWSVSMNAYAYGKFAPGRCSDWLNLNCTGGDSGTEPYLTAHYQLLAHAAATKLYKTKYQASQYGIIGITLLSHWYEPASQAKADVDAAQRGLDFMFGWYMDPITKGNYPKSMRSLVGSRLPKFSKEESEDLKGSFDFLGLNYYSSSYAADAPHLRDARPAIQTDSLINATFHRDGKPLGPMSASSWLCIYPRGLGNLLLYVKNKYDNPIIYITENGRDEFNDPKLSLEESLLDTYRIDYFYRHLYYVQAAIREGVNVKGYFAWSLLDNHEWESGFSLRFGLVFVDYNDDLKRHPKLSAHWFKNFLKKSCQEKCSV*