AlfalfaGEDB | Msa0187900

M. sativa cultivar zhongmu-4 / Msa0187900

KEGG: K09756|K16296
GO: -
PFAM: PF00450.25

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0187900	KEH36682.1	82.639	144	7	1	19	144	217	360	4.74e-78	245

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0187900	sp\|O64811\|SCP9_ARATH	55.245	143	45	2	20	144	296	437	1.53e-44	154

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0187900	A0A072V4C5	82.639	144	7	1	19	144	217	360	2.26e-78	245

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0030590	Msa0187900	0.814297	1.654891e-51	-8.615850e-47
Msa0125440	Msa0187900	0.801354	9.369106e-49	-8.615850e-47
Msa0128370	Msa0187900	0.817513	3.171342e-52	-8.615850e-47
Msa0168100	Msa0187900	0.818161	2.264249e-52	-8.615850e-47
Msa0174560	Msa0187900	0.805863	1.086769e-49	-8.615850e-47
Msa0187900	Msa0194510	0.970902	2.504401e-132	-8.615850e-47
Msa0187900	Msa0207180	0.806528	7.875263e-50	-8.615850e-47
Msa0187900	Msa0260970	0.805318	1.414271e-49	-8.615850e-47
Msa0187900	Msa0351230	0.806403	8.368709e-50	-8.615850e-47
Msa0187900	Msa0371610	0.815399	9.431960e-52	-8.615850e-47
Msa0187900	Msa0413740	0.815610	8.462660e-52	-8.615850e-47
Msa0187900	Msa0430820	0.809260	2.066039e-50	-8.615850e-47
Msa0187900	Msa0505390	0.816434	5.540978e-52	-8.615850e-47
Msa0187900	Msa0525240	0.802519	5.399064e-49	-8.615850e-47
Msa0187900	Msa0530930	0.804055	2.595027e-49	-8.615850e-47
Msa0187900	Msa0581340	0.805178	1.513211e-49	-8.615850e-47
Msa0187900	Msa0583590	0.811653	6.286382e-51	-8.615850e-47
Msa0187900	Msa0626230	0.815976	7.015059e-52	-8.615850e-47
Msa0187900	Msa0756480	0.804219	2.399032e-49	-8.615850e-47
Msa0187900	Msa0820670	0.801351	9.382190e-49	-8.615850e-47
Msa0187900	Msa0890250	0.801636	8.198921e-49	-8.615850e-47
Msa0187900	Msa0950620	0.810604	1.061485e-50	-8.615850e-47
Msa0187900	Msa0951720	0.802348	5.853710e-49	-8.615850e-47
Msa0187900	Msa0984720	0.804579	2.018455e-49	-8.615850e-47
Msa0187900	Msa1018720	0.803758	2.991883e-49	-8.615850e-47
Msa0187900	Msa1021770	0.806024	1.005613e-49	-8.615850e-47
Msa0187900	Msa1036390	0.802816	4.688076e-49	-8.615850e-47
Msa0187900	Msa1055280	0.806720	7.171447e-50	-8.615850e-47
Msa0187900	Msa1076680	0.810156	1.326019e-50	-8.615850e-47
Msa0187900	Msa1184580	0.800754	1.242368e-48	-8.615850e-47
Msa0187900	Msa1214210	0.800649	1.305407e-48	-8.615850e-47
Msa0187900	Msa1221260	0.803190	3.923415e-49	-8.615850e-47
Msa0187900	Msa1238480	0.819902	9.099650e-53	-8.615850e-47
Msa0187900	Msa1329990	0.805953	1.040493e-49	-8.615850e-47
Msa0187900	Msa1379070	0.812340	4.452743e-51	-8.615850e-47
Msa0187900	Msa1379090	0.810046	1.400298e-50	-8.615850e-47
Msa0187900	Msa1397540	0.822105	2.830611e-53	-8.615850e-47
Msa0187900	Msa1435040	0.807498	4.909331e-50	-8.615850e-47
Msa0187900	Msa1445790	0.804452	2.146075e-49	-8.615850e-47
Msa0187900	Msa1448720	0.852573	4.223570e-61	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0187900	MtrunA17_Chr2g0285381	82.639	144	7	1	19	144	322	465	4.38e-81	246
Msa0187900	MtrunA17_Chr3g0133011	51.744	172	54	2	2	144	325	496	1.96e-54	178
Msa0187900	MtrunA17_Chr2g0285351	58.333	144	42	1	19	144	326	469	1.61e-53	175
Msa0187900	MtrunA17_Chr2g0285371	59.722	144	40	1	19	144	326	469	1.69e-53	175
Msa0187900	MtrunA17_Chr4g0009641	54.545	143	46	2	20	144	305	446	2.86e-47	158
Msa0187900	MtrunA17_Chr4g0009661	51.748	143	50	2	20	144	329	470	1.03e-43	149
Msa0187900	MtrunA17_Chr4g0009721	50.685	146	49	3	19	144	277	419	1.68e-43	147
Msa0187900	MtrunA17_Chr4g0009701	51.799	139	48	2	24	144	333	470	2.53e-43	148
Msa0187900	MtrunA17_Chr4g0009651	51.049	143	51	2	20	144	329	470	1.58e-42	146
Msa0187900	MtrunA17_Chr4g0009681	51.079	139	49	2	24	144	330	467	2.65e-41	142
Msa0187900	MtrunA17_Chr4g0009671	49.640	139	51	2	24	144	298	435	1.02e-39	138
Msa0187900	MtrunA17_Chr4g0009711	46.528	144	58	2	19	144	328	470	2.33e-38	135
Msa0187900	MtrunA17_Chr2g0285401	75.000	68	17	0	55	122	19	86	1.32e-32	110
Msa0187900	MtrunA17_Chr7g0249431	34.320	169	78	3	8	144	331	498	1.88e-30	114
Msa0187900	MtrunA17_Chr8g0338461	35.366	164	74	4	12	144	333	495	3.97e-30	113
Msa0187900	MtrunA17_Chr3g0093971	38.194	144	67	1	19	140	307	450	3.78e-28	107
Msa0187900	MtrunA17_Chr7g0234051	35.714	140	71	3	24	144	356	495	6.79e-26	101
Msa0187900	MtrunA17_Chr7g0234041	41.905	105	50	1	28	121	340	444	3.77e-22	90.9
Msa0187900	MtrunA17_Chr2g0279381	38.235	102	57	1	19	120	116	211	9.20e-20	81.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0187900	AT2G23010.1	55.245	143	45	2	20	144	296	437	1.56e-45	154
Msa0187900	AT2G23010.2	53.521	142	49	1	20	144	296	437	1.59e-44	151
Msa0187900	AT2G22990.6	53.147	143	48	2	20	144	178	319	2.48e-44	148
Msa0187900	AT2G22960.1	53.237	139	46	2	24	144	47	184	2.48e-44	144
Msa0187900	AT5G36180.5	53.147	143	48	2	20	144	198	339	3.27e-44	148
Msa0187900	AT5G36180.3	53.147	143	48	2	20	144	198	339	3.27e-44	148
Msa0187900	AT5G36180.1	53.147	143	48	2	20	144	300	441	2.08e-43	148
Msa0187900	AT2G22990.2	51.408	142	52	1	20	144	178	319	2.17e-43	145
Msa0187900	AT1G73300.1	52.448	143	49	2	20	144	300	441	2.26e-43	148
Msa0187900	AT1G73300.2	52.448	143	49	2	20	144	300	441	2.26e-43	148
Msa0187900	AT2G22990.1	53.147	143	48	2	20	144	292	433	2.31e-43	148
Msa0187900	AT1G73280.3	51.748	143	50	2	20	144	173	314	4.37e-43	144
Msa0187900	AT1G73280.4	51.748	143	50	2	20	144	198	339	6.02e-43	145
Msa0187900	AT1G73310.1	50.000	152	57	2	11	144	291	441	1.29e-42	146
Msa0187900	AT2G22920.3	50.345	145	51	3	20	144	113	256	1.65e-42	141
Msa0187900	AT2G22990.5	51.408	142	52	1	20	144	283	424	2.12e-42	145
Msa0187900	AT1G73280.1	51.748	143	50	2	20	144	300	441	4.14e-42	145
Msa0187900	AT3G10450.2	49.650	143	53	2	20	144	253	394	3.23e-41	141
Msa0187900	AT3G10450.1	49.650	143	53	2	20	144	296	437	7.58e-41	141
Msa0187900	AT2G23000.1	53.147	143	48	2	20	144	296	437	7.74e-41	141
Msa0187900	AT2G23000.2	53.147	143	48	2	20	144	296	437	7.74e-41	141
Msa0187900	AT2G22920.2	50.345	145	51	3	20	144	292	435	8.77e-41	141
Msa0187900	AT1G73290.1	50.350	143	52	2	20	144	297	438	1.00e-40	141
Msa0187900	AT3G12203.2	49.640	139	51	2	24	144	242	379	3.13e-40	139
Msa0187900	AT3G12203.3	49.640	139	51	2	24	144	279	416	6.21e-40	139
Msa0187900	AT3G12203.1	49.640	139	51	2	24	144	300	437	7.54e-40	139
Msa0187900	AT1G33540.2	50.694	144	51	3	20	144	279	421	3.16e-39	137
Msa0187900	AT1G33540.1	50.694	144	51	3	20	144	322	464	7.36e-39	136
Msa0187900	AT2G22970.1	49.306	144	53	3	20	144	291	433	1.13e-38	135
Msa0187900	AT5G36180.2	60.194	103	41	0	20	122	198	300	1.13e-38	133
Msa0187900	AT5G36180.4	57.407	108	46	0	20	127	198	305	2.69e-38	132
Msa0187900	AT5G09640.1	49.655	145	51	4	20	144	323	465	3.70e-38	135
Msa0187900	AT2G22980.1	50.000	140	50	3	24	144	292	430	3.06e-37	132
Msa0187900	AT1G73310.3	55.856	111	49	0	11	121	189	299	5.85e-37	129
Msa0187900	AT3G12240.1	47.222	144	57	2	19	144	294	436	1.23e-36	130
Msa0187900	AT1G73270.2	48.921	139	52	2	24	144	304	441	1.92e-36	130
Msa0187900	AT1G73270.1	48.921	139	52	2	24	144	304	441	2.00e-36	130
Msa0187900	AT2G22990.4	53.600	125	55	2	20	142	292	415	3.18e-36	129
Msa0187900	AT1G73310.2	55.856	111	49	0	11	121	291	401	3.71e-36	129
Msa0187900	AT2G22990.3	52.846	123	57	1	20	142	292	413	4.11e-36	129
Msa0187900	AT3G12230.1	48.611	144	55	2	19	144	293	435	5.26e-36	128
Msa0187900	AT2G22920.1	56.731	104	43	1	20	121	292	395	8.89e-36	127
Msa0187900	AT1G73290.3	56.863	102	44	0	20	121	297	398	3.30e-35	126
Msa0187900	AT3G10450.3	54.369	103	47	0	20	122	253	355	1.64e-34	123
Msa0187900	AT3G10450.4	54.369	103	47	0	20	122	296	398	3.30e-34	123
Msa0187900	AT2G22970.4	56.731	104	44	1	20	122	291	394	5.13e-34	123
Msa0187900	AT2G22970.3	56.731	104	44	1	20	122	291	394	6.55e-34	123
Msa0187900	AT2G22980.5	54.808	104	46	1	20	122	171	274	2.71e-32	116
Msa0187900	AT3G12220.3	43.537	147	64	2	16	144	238	383	3.07e-32	117
Msa0187900	AT3G12220.2	43.750	144	62	2	19	144	263	405	7.63e-32	117
Msa0187900	AT3G12220.1	43.750	144	62	2	19	144	293	435	1.21e-31	117
Msa0187900	AT2G22980.2	56.000	100	43	1	24	122	292	391	2.23e-31	115
Msa0187900	AT2G22980.4	56.000	100	43	1	24	122	292	391	3.05e-31	115
Msa0187900	AT4G12910.1	34.356	163	76	4	8	139	330	492	7.96e-28	107
Msa0187900	AT3G25420.1	35.606	132	66	2	28	140	359	490	2.74e-26	103
Msa0187900	AT4G12910.2	35.714	126	68	2	8	120	330	455	3.55e-23	94.4
Msa0187900	AT1G73280.2	59.322	59	24	0	23	81	303	361	4.54e-15	71.2
Msa0187900	AT2G22980.3	54.237	59	26	1	24	81	294	352	2.03e-11	60.8
Msa0187900	AT2G05850.1	32.727	110	49	2	28	122	344	443	4.73e-11	59.7
Msa0187900	AT5G23210.5	28.049	164	94	5	5	144	236	399	9.94e-11	58.9

Find 36 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACAATTACTTAGTGATTAT+TGG	0.137789	2_1:+2717086	Msa0187900:CDS
TGACATTTCTATTCCATTCT+TGG	0.177100	2_1:+2717554	Msa0187900:CDS
TTCTGATAAAATACCTTATA+CGG	0.269525	2_1:+2717255	Msa0187900:CDS
ACAATTACTTAGTGATTATT+GGG	0.304493	2_1:+2717087	Msa0187900:CDS
ACTACTCCATTGTTGATGAT+TGG	0.342996	2_1:+2717604	Msa0187900:CDS
TGCAGGGAACTATTGGAAAA+TGG	0.362864	2_1:+2717224	Msa0187900:intron
TTAGAGATATCTCCCGTATA+AGG	0.370677	2_1:-2717268	None:intergenic
CATGTAAATCTCAGTGATAA+AGG	0.422636	2_1:+2717304	Msa0187900:CDS
CATGTGTTGCAGGGAACTAT+TGG	0.422923	2_1:+2717217	Msa0187900:intron
GATATCTAAGAGGCCTTTGT+AGG	0.428982	2_1:+2718221	Msa0187900:CDS
TTCGCGAACAGTGCTATTGT+TGG	0.450789	2_1:-2717110	None:intergenic
ATCATGGCAAGACATTCTTC+AGG	0.466282	2_1:-2718189	None:intergenic
GTCTTGCCATGATTAGTAGA+TGG	0.467692	2_1:+2718199	Msa0187900:CDS
AGATATCCATCTACTAATCA+TGG	0.472745	2_1:-2718205	None:intergenic
CAATAATCACTAAGTAATTG+TGG	0.477111	2_1:-2717085	None:intergenic
GACACACAGCTGCCGAGTAC+CGG	0.492510	2_1:+2718166	Msa0187900:CDS
ACTCCATTGTTGATGATTGG+AGG	0.510049	2_1:+2717607	Msa0187900:CDS
AATAATCACTAAGTAATTGT+GGG	0.522377	2_1:-2717084	None:intergenic
GCTACCGTTCACTAATCTAC+AGG	0.535329	2_1:+2717326	Msa0187900:CDS
ACATACCTTGCGTATGTGTA+GGG	0.537590	2_1:-2717134	None:intergenic
TGATCAAATTTACAGGGTGG+AGG	0.552936	2_1:+2718144	Msa0187900:intron
AACCCTCAATGTCGAATGAA+AGG	0.553128	2_1:+2716813	None:intergenic
TCTGATAAAATACCTTATAC+GGG	0.558066	2_1:+2717256	Msa0187900:CDS
ACATTCTTCAGGCCGGTACT+CGG	0.562500	2_1:-2718178	None:intergenic
TACATACCTTGCGTATGTGT+AGG	0.573629	2_1:-2717135	None:intergenic
TTGTTGATGATTGGAGGCAG+TGG	0.574543	2_1:+2717613	Msa0187900:CDS
TTAGTAGATGGATATCTAAG+AGG	0.578752	2_1:+2718211	Msa0187900:CDS
CATTCTTGGATACAAAAGCA+TGG	0.590619	2_1:+2717568	Msa0187900:CDS
TGGCAAGACATTCTTCAGGC+CGG	0.619227	2_1:-2718185	None:intergenic
TGGAGGCAGTGGCATACAGA+GGG	0.634579	2_1:+2717624	Msa0187900:CDS
CTGCCTCCAATCATCAACAA+TGG	0.635469	2_1:-2717610	None:intergenic
TTGACCTGTAGATTAGTGAA+CGG	0.637534	2_1:-2717330	None:intergenic
AAAGGAAGGTAATCTCATGA+CGG	0.662772	2_1:+2716853	Msa0187900:CDS
TTGGAGGCAGTGGCATACAG+AGG	0.686415	2_1:+2717623	Msa0187900:CDS
CGAAGCCCTACACATACGCA+AGG	0.686482	2_1:+2717129	Msa0187900:CDS
AAATTGAGGGGAGAAAAGGA+AGG	0.693386	2_1:+2716839	Msa0187900:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTATATATTGTTAACAG+TGG	+	chr2_1:2717525-2717544	Msa0187900:intron	15.0%
!!	ATATCTTATAAATAGTTGAA+GGG	-	chr2_1:2717810-2717829	None:intergenic	15.0%
!!	TATATCTTATAAATAGTTGA+AGG	-	chr2_1:2717811-2717830	None:intergenic	15.0%
!!!	TGCATATTGTTAAATTTTTA+GGG	-	chr2_1:2718114-2718133	None:intergenic	15.0%
!!!	TTGCATATTGTTAAATTTTT+AGG	-	chr2_1:2718115-2718134	None:intergenic	15.0%
!!!	ATTTAACAATATGCAATTTT+TGG	+	chr2_1:2718119-2718138	Msa0187900:intron	15.0%
!!!	ATTTTATATATTGTTAACAG+TGG	+	chr2_1:2717525-2717544	Msa0187900:intron	15.0%
!!	ATATCTTATAAATAGTTGAA+GGG	-	chr2_1:2717810-2717829	None:intergenic	15.0%
!!	TATATCTTATAAATAGTTGA+AGG	-	chr2_1:2717811-2717830	None:intergenic	15.0%
!!!	TGCATATTGTTAAATTTTTA+GGG	-	chr2_1:2718114-2718133	None:intergenic	15.0%
!!!	TTGCATATTGTTAAATTTTT+AGG	-	chr2_1:2718115-2718134	None:intergenic	15.0%
!!!	ATTTAACAATATGCAATTTT+TGG	+	chr2_1:2718119-2718138	Msa0187900:intron	15.0%
!!	TGATCGTTTGATTAAAATTA+AGG	-	chr2_1:2716932-2716951	None:intergenic	20.0%
!!!	TATTTTGTATTTTTCACGAT+AGG	+	chr2_1:2716959-2716978	Msa0187900:intron	20.0%
!!	AGGTTATATATATAGATTAG+GGG	-	chr2_1:2717016-2717035	None:intergenic	20.0%
!!	GAGGTTATATATATAGATTA+GGG	-	chr2_1:2717017-2717036	None:intergenic	20.0%
!!	AATAATCACTAAGTAATTGT+GGG	-	chr2_1:2717087-2717106	None:intergenic	20.0%
!!	ACAATTACTTAGTGATTATT+GGG	+	chr2_1:2717087-2717106	Msa0187900:CDS	20.0%
!!	TTCTGATAAAATACCTTATA+CGG	+	chr2_1:2717255-2717274	Msa0187900:CDS	20.0%
!!!	TTCTTTTTTCTTTTCTGAAA+TGG	+	chr2_1:2717727-2717746	Msa0187900:intron	20.0%
!!	TGATCGTTTGATTAAAATTA+AGG	-	chr2_1:2716932-2716951	None:intergenic	20.0%
!!!	TATTTTGTATTTTTCACGAT+AGG	+	chr2_1:2716959-2716978	Msa0187900:intron	20.0%
!!	AGGTTATATATATAGATTAG+GGG	-	chr2_1:2717016-2717035	None:intergenic	20.0%
!!	GAGGTTATATATATAGATTA+GGG	-	chr2_1:2717017-2717036	None:intergenic	20.0%
!!	AATAATCACTAAGTAATTGT+GGG	-	chr2_1:2717087-2717106	None:intergenic	20.0%
!!	ACAATTACTTAGTGATTATT+GGG	+	chr2_1:2717087-2717106	Msa0187900:CDS	20.0%
!!	TTCTGATAAAATACCTTATA+CGG	+	chr2_1:2717255-2717274	Msa0187900:CDS	20.0%
!!!	TTCTTTTTTCTTTTCTGAAA+TGG	+	chr2_1:2717727-2717746	Msa0187900:intron	20.0%
!	GAATGAAAGGAAAAAATTGA+GGG	+	chr2_1:2716826-2716845	Msa0187900:CDS	25.0%
!	AATGAAAGGAAAAAATTGAG+GGG	+	chr2_1:2716827-2716846	Msa0187900:CDS	25.0%
!	CGAGGTTATATATATAGATT+AGG	-	chr2_1:2717018-2717037	None:intergenic	25.0%
!	CAATAATCACTAAGTAATTG+TGG	-	chr2_1:2717088-2717107	None:intergenic	25.0%
!	CACAATTACTTAGTGATTAT+TGG	+	chr2_1:2717086-2717105	Msa0187900:CDS	25.0%
!	GTGTCTATATATGTATGTAT+GGG	-	chr2_1:2717170-2717189	None:intergenic	25.0%
!	TGTGTCTATATATGTATGTA+TGG	-	chr2_1:2717171-2717190	None:intergenic	25.0%
!	TCTGATAAAATACCTTATAC+GGG	+	chr2_1:2717256-2717275	Msa0187900:CDS	25.0%
!	CTATGTTCATAAATTGCATA+TGG	+	chr2_1:2717363-2717382	Msa0187900:intron	25.0%
!	TCAACAAACGTTAATGTATT+TGG	+	chr2_1:2717845-2717864	Msa0187900:intron	25.0%
!	GAATGAAAGGAAAAAATTGA+GGG	+	chr2_1:2716826-2716845	Msa0187900:CDS	25.0%
!	AATGAAAGGAAAAAATTGAG+GGG	+	chr2_1:2716827-2716846	Msa0187900:CDS	25.0%
!	CGAGGTTATATATATAGATT+AGG	-	chr2_1:2717018-2717037	None:intergenic	25.0%
!	CAATAATCACTAAGTAATTG+TGG	-	chr2_1:2717088-2717107	None:intergenic	25.0%
!	CACAATTACTTAGTGATTAT+TGG	+	chr2_1:2717086-2717105	Msa0187900:CDS	25.0%
!	GTGTCTATATATGTATGTAT+GGG	-	chr2_1:2717170-2717189	None:intergenic	25.0%
!	TGTGTCTATATATGTATGTA+TGG	-	chr2_1:2717171-2717190	None:intergenic	25.0%
!	TCTGATAAAATACCTTATAC+GGG	+	chr2_1:2717256-2717275	Msa0187900:CDS	25.0%
!	CTATGTTCATAAATTGCATA+TGG	+	chr2_1:2717363-2717382	Msa0187900:intron	25.0%
!	TCAACAAACGTTAATGTATT+TGG	+	chr2_1:2717845-2717864	Msa0187900:intron	25.0%
	CGAATGAAAGGAAAAAATTG+AGG	+	chr2_1:2716825-2716844	Msa0187900:CDS	30.0%
	CAAGTTAACAAAATGTTTCG+AGG	-	chr2_1:2717036-2717055	None:intergenic	30.0%
	TAACTTGATGTTTGTTTCCT+CGG	+	chr2_1:2717049-2717068	Msa0187900:intron	30.0%
	CATGTAAATCTCAGTGATAA+AGG	+	chr2_1:2717304-2717323	Msa0187900:CDS	30.0%
	TGACATTTCTATTCCATTCT+TGG	+	chr2_1:2717554-2717573	Msa0187900:CDS	30.0%
	AATTATAGTTGTAGCAGTTG+AGG	-	chr2_1:2718034-2718053	None:intergenic	30.0%
!!	TTTGGCTTGATCAAATTTAC+AGG	+	chr2_1:2718137-2718156	Msa0187900:intron	30.0%
!	TTGGCTTGATCAAATTTACA+GGG	+	chr2_1:2718138-2718157	Msa0187900:intron	30.0%
	AGATATCCATCTACTAATCA+TGG	-	chr2_1:2718208-2718227	None:intergenic	30.0%
	TTAGTAGATGGATATCTAAG+AGG	+	chr2_1:2718211-2718230	Msa0187900:CDS	30.0%
	CGAATGAAAGGAAAAAATTG+AGG	+	chr2_1:2716825-2716844	Msa0187900:CDS	30.0%
	CAAGTTAACAAAATGTTTCG+AGG	-	chr2_1:2717036-2717055	None:intergenic	30.0%
	TAACTTGATGTTTGTTTCCT+CGG	+	chr2_1:2717049-2717068	Msa0187900:intron	30.0%
	CATGTAAATCTCAGTGATAA+AGG	+	chr2_1:2717304-2717323	Msa0187900:CDS	30.0%
	TGACATTTCTATTCCATTCT+TGG	+	chr2_1:2717554-2717573	Msa0187900:CDS	30.0%
	AATTATAGTTGTAGCAGTTG+AGG	-	chr2_1:2718034-2718053	None:intergenic	30.0%
!!	TTTGGCTTGATCAAATTTAC+AGG	+	chr2_1:2718137-2718156	Msa0187900:intron	30.0%
!	TTGGCTTGATCAAATTTACA+GGG	+	chr2_1:2718138-2718157	Msa0187900:intron	30.0%
	AGATATCCATCTACTAATCA+TGG	-	chr2_1:2718208-2718227	None:intergenic	30.0%
	TTAGTAGATGGATATCTAAG+AGG	+	chr2_1:2718211-2718230	Msa0187900:CDS	30.0%
	GAAAAAATTGAGGGGAGAAA+AGG	+	chr2_1:2716835-2716854	Msa0187900:CDS	35.0%
	AAAGGAAGGTAATCTCATGA+CGG	+	chr2_1:2716853-2716872	Msa0187900:CDS	35.0%
	TTAGAGATATCTCCCGTATA+AGG	-	chr2_1:2717271-2717290	None:intergenic	35.0%
	TTGACCTGTAGATTAGTGAA+CGG	-	chr2_1:2717333-2717352	None:intergenic	35.0%
!	CATGCTTTTGTATCCAAGAA+TGG	-	chr2_1:2717570-2717589	None:intergenic	35.0%
	CATTCTTGGATACAAAAGCA+TGG	+	chr2_1:2717568-2717587	Msa0187900:CDS	35.0%
!!	ACTACTCCATTGTTGATGAT+TGG	+	chr2_1:2717604-2717623	Msa0187900:CDS	35.0%
	TGTGCAAGTATAGATACACA+AGG	+	chr2_1:2717958-2717977	Msa0187900:intron	35.0%
!!	ATTTTTAGGGAAACTCAGTG+TGG	-	chr2_1:2718101-2718120	None:intergenic	35.0%
	GAAAAAATTGAGGGGAGAAA+AGG	+	chr2_1:2716835-2716854	Msa0187900:CDS	35.0%
	AAAGGAAGGTAATCTCATGA+CGG	+	chr2_1:2716853-2716872	Msa0187900:CDS	35.0%
	TTAGAGATATCTCCCGTATA+AGG	-	chr2_1:2717271-2717290	None:intergenic	35.0%
	TTGACCTGTAGATTAGTGAA+CGG	-	chr2_1:2717333-2717352	None:intergenic	35.0%
!	CATGCTTTTGTATCCAAGAA+TGG	-	chr2_1:2717570-2717589	None:intergenic	35.0%
	CATTCTTGGATACAAAAGCA+TGG	+	chr2_1:2717568-2717587	Msa0187900:CDS	35.0%
!!	ACTACTCCATTGTTGATGAT+TGG	+	chr2_1:2717604-2717623	Msa0187900:CDS	35.0%
	TGTGCAAGTATAGATACACA+AGG	+	chr2_1:2717958-2717977	Msa0187900:intron	35.0%
!!	ATTTTTAGGGAAACTCAGTG+TGG	-	chr2_1:2718101-2718120	None:intergenic	35.0%
	AAATTGAGGGGAGAAAAGGA+AGG	+	chr2_1:2716839-2716858	Msa0187900:CDS	40.0%
	ACATACCTTGCGTATGTGTA+GGG	-	chr2_1:2717137-2717156	None:intergenic	40.0%
	TACATACCTTGCGTATGTGT+AGG	-	chr2_1:2717138-2717157	None:intergenic	40.0%
	CACACACGATAATACTCGTT+TGG	+	chr2_1:2717191-2717210	Msa0187900:intron	40.0%
	TGCAGGGAACTATTGGAAAA+TGG	+	chr2_1:2717224-2717243	Msa0187900:intron	40.0%
!!	ACTCCATTGTTGATGATTGG+AGG	+	chr2_1:2717607-2717626	Msa0187900:CDS	40.0%
	TATGTGAATACCCTGCAACT+TGG	-	chr2_1:2717650-2717669	None:intergenic	40.0%
	TACACAAGGACATACTCGAA+TGG	+	chr2_1:2717972-2717991	Msa0187900:intron	40.0%
	ACACAAGGACATACTCGAAT+GGG	+	chr2_1:2717973-2717992	Msa0187900:intron	40.0%
	GATGACATTTGCAACTGTGA+AGG	+	chr2_1:2717995-2718014	Msa0187900:intron	40.0%
	GCTTGATCAAATTTACAGGG+TGG	+	chr2_1:2718141-2718160	Msa0187900:intron	40.0%
	TGATCAAATTTACAGGGTGG+AGG	+	chr2_1:2718144-2718163	Msa0187900:intron	40.0%
	ATCATGGCAAGACATTCTTC+AGG	-	chr2_1:2718192-2718211	None:intergenic	40.0%
	GTCTTGCCATGATTAGTAGA+TGG	+	chr2_1:2718199-2718218	Msa0187900:CDS	40.0%
	AAATTGAGGGGAGAAAAGGA+AGG	+	chr2_1:2716839-2716858	Msa0187900:CDS	40.0%
	ACATACCTTGCGTATGTGTA+GGG	-	chr2_1:2717137-2717156	None:intergenic	40.0%
	TACATACCTTGCGTATGTGT+AGG	-	chr2_1:2717138-2717157	None:intergenic	40.0%
	CACACACGATAATACTCGTT+TGG	+	chr2_1:2717191-2717210	Msa0187900:intron	40.0%
	TGCAGGGAACTATTGGAAAA+TGG	+	chr2_1:2717224-2717243	Msa0187900:intron	40.0%
!!	ACTCCATTGTTGATGATTGG+AGG	+	chr2_1:2717607-2717626	Msa0187900:CDS	40.0%
	TATGTGAATACCCTGCAACT+TGG	-	chr2_1:2717650-2717669	None:intergenic	40.0%
	TACACAAGGACATACTCGAA+TGG	+	chr2_1:2717972-2717991	Msa0187900:intron	40.0%
	ACACAAGGACATACTCGAAT+GGG	+	chr2_1:2717973-2717992	Msa0187900:intron	40.0%
	GATGACATTTGCAACTGTGA+AGG	+	chr2_1:2717995-2718014	Msa0187900:intron	40.0%
	GCTTGATCAAATTTACAGGG+TGG	+	chr2_1:2718141-2718160	Msa0187900:intron	40.0%
	TGATCAAATTTACAGGGTGG+AGG	+	chr2_1:2718144-2718163	Msa0187900:intron	40.0%
	ATCATGGCAAGACATTCTTC+AGG	-	chr2_1:2718192-2718211	None:intergenic	40.0%
	GTCTTGCCATGATTAGTAGA+TGG	+	chr2_1:2718199-2718218	Msa0187900:CDS	40.0%
	GTGGGTAATTCTAACATCCG+AGG	-	chr2_1:2717069-2717088	None:intergenic	45.0%
!	TTCGCGAACAGTGCTATTGT+TGG	-	chr2_1:2717113-2717132	None:intergenic	45.0%
	GTTTGGTTGCATGTGTTGCA+GGG	+	chr2_1:2717208-2717227	Msa0187900:intron	45.0%
	CATGTGTTGCAGGGAACTAT+TGG	+	chr2_1:2717217-2717236	Msa0187900:intron	45.0%
	GCTACCGTTCACTAATCTAC+AGG	+	chr2_1:2717326-2717345	Msa0187900:CDS	45.0%
	CTGCCTCCAATCATCAACAA+TGG	-	chr2_1:2717613-2717632	None:intergenic	45.0%
!!	TTGTTGATGATTGGAGGCAG+TGG	+	chr2_1:2717613-2717632	Msa0187900:CDS	45.0%
	GTGGGTAATTCTAACATCCG+AGG	-	chr2_1:2717069-2717088	None:intergenic	45.0%
!	TTCGCGAACAGTGCTATTGT+TGG	-	chr2_1:2717113-2717132	None:intergenic	45.0%
	GTTTGGTTGCATGTGTTGCA+GGG	+	chr2_1:2717208-2717227	Msa0187900:intron	45.0%
	CATGTGTTGCAGGGAACTAT+TGG	+	chr2_1:2717217-2717236	Msa0187900:intron	45.0%
	GCTACCGTTCACTAATCTAC+AGG	+	chr2_1:2717326-2717345	Msa0187900:CDS	45.0%
	CTGCCTCCAATCATCAACAA+TGG	-	chr2_1:2717613-2717632	None:intergenic	45.0%
!!	TTGTTGATGATTGGAGGCAG+TGG	+	chr2_1:2717613-2717632	Msa0187900:CDS	45.0%
	CGTTTGGTTGCATGTGTTGC+AGG	+	chr2_1:2717207-2717226	Msa0187900:intron	50.0%
	ATACAGAGGGCCAAGTTGCA+GGG	+	chr2_1:2717637-2717656	Msa0187900:intron	50.0%
	ACATTCTTCAGGCCGGTACT+CGG	-	chr2_1:2718181-2718200	None:intergenic	50.0%
	TGGCAAGACATTCTTCAGGC+CGG	-	chr2_1:2718188-2718207	None:intergenic	50.0%
	CGTTTGGTTGCATGTGTTGC+AGG	+	chr2_1:2717207-2717226	Msa0187900:intron	50.0%
	ATACAGAGGGCCAAGTTGCA+GGG	+	chr2_1:2717637-2717656	Msa0187900:intron	50.0%
	ACATTCTTCAGGCCGGTACT+CGG	-	chr2_1:2718181-2718200	None:intergenic	50.0%
	TGGCAAGACATTCTTCAGGC+CGG	-	chr2_1:2718188-2718207	None:intergenic	50.0%
	CGAAGCCCTACACATACGCA+AGG	+	chr2_1:2717129-2717148	Msa0187900:CDS	55.0%
	TTGGAGGCAGTGGCATACAG+AGG	+	chr2_1:2717623-2717642	Msa0187900:CDS	55.0%
	TGGAGGCAGTGGCATACAGA+GGG	+	chr2_1:2717624-2717643	Msa0187900:CDS	55.0%
	CATACAGAGGGCCAAGTTGC+AGG	+	chr2_1:2717636-2717655	Msa0187900:intron	55.0%
	CGAAGCCCTACACATACGCA+AGG	+	chr2_1:2717129-2717148	Msa0187900:CDS	55.0%
	TTGGAGGCAGTGGCATACAG+AGG	+	chr2_1:2717623-2717642	Msa0187900:CDS	55.0%
	TGGAGGCAGTGGCATACAGA+GGG	+	chr2_1:2717624-2717643	Msa0187900:CDS	55.0%
	CATACAGAGGGCCAAGTTGC+AGG	+	chr2_1:2717636-2717655	Msa0187900:intron	55.0%
	GACACACAGCTGCCGAGTAC+CGG	+	chr2_1:2718166-2718185	Msa0187900:CDS	60.0%
	GACACACAGCTGCCGAGTAC+CGG	+	chr2_1:2718166-2718185	Msa0187900:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	2716821	2718242	2716821	ID=Msa0187900;Name=Msa0187900
chr2_1	mRNA	2716821	2718242	2716821	ID=Msa0187900-mRNA-1;Parent=Msa0187900;Name=Msa0187900-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0\|0.25\|0\|0.8\|1\|1\|5\|0\|144
chr2_1	exon	2716821	2716874	2716821	ID=Msa0187900-mRNA-1:exon:1253;Parent=Msa0187900-mRNA-1
chr2_1	exon	2717079	2717150	2717079	ID=Msa0187900-mRNA-1:exon:1254;Parent=Msa0187900-mRNA-1
chr2_1	exon	2717229	2717347	2717229	ID=Msa0187900-mRNA-1:exon:1255;Parent=Msa0187900-mRNA-1
chr2_1	exon	2717545	2717650	2717545	ID=Msa0187900-mRNA-1:exon:1256;Parent=Msa0187900-mRNA-1
chr2_1	exon	2718159	2718242	2718159	ID=Msa0187900-mRNA-1:exon:1257;Parent=Msa0187900-mRNA-1
chr2_1	CDS	2716821	2716874	2716821	ID=Msa0187900-mRNA-1:cds;Parent=Msa0187900-mRNA-1
chr2_1	CDS	2717079	2717150	2717079	ID=Msa0187900-mRNA-1:cds;Parent=Msa0187900-mRNA-1
chr2_1	CDS	2717229	2717347	2717229	ID=Msa0187900-mRNA-1:cds;Parent=Msa0187900-mRNA-1
chr2_1	CDS	2717545	2717650	2717545	ID=Msa0187900-mRNA-1:cds;Parent=Msa0187900-mRNA-1
chr2_1	CDS	2718159	2718242	2718159	ID=Msa0187900-mRNA-1:cds;Parent=Msa0187900-mRNA-1

Gene Sequence

>Msa0187900

ATGTCGAATGAAAGGAAAAAATTGAGGGGAGAAAAGGAAGGTAATCTCATGACGAATTACCCACAATTACTTAGTGATTATTGGGCCAACAATAGCACTGTTCGCGAAGCCCTACACATACGCAAGGGAACTATTGGAAAATGGAAACGTCGTTCTGATAAAATACCTTATACGGGAGATATCTCTAACAGCTTCGATTATCATGTAAATCTCAGTGATAAAGGCTACCGTTCACTAATCTACAGTGGCGATCATGACATTTCTATTCCATTCTTGGATACAAAAGCATGGATAAAATCTTTAAACTACTCCATTGTTGATGATTGGAGGCAGTGGCATACAGAGGGCCAAGGTGGAGGACACACAGCTGCCGAGTACCGGCCTGAAGAATGTCTTGCCATGATTAGTAGATGGATATCTAAGAGGCCTTTGTAG

Protein sequence

>Msa0187900

MSNERKKLRGEKEGNLMTNYPQLLSDYWANNSTVREALHIRKGTIGKWKRRSDKIPYTGDISNSFDYHVNLSDKGYRSLIYSGDHDISIPFLDTKAWIKSLNYSIVDDWRQWHTEGQGGGHTAAEYRPEECLAMISRWISKRPL*