AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0530930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0530930 MtrunA17_Chr4g0003891 85.593 236 12 2 1 214 22 257 9.58e-141 405
Msa0530930 MtrunA17_Chr2g0332281 69.767 215 41 2 24 214 23 237 4.86e-100 300
Msa0530930 MtrunA17_Chr4g0050021 33.840 263 106 4 25 220 230 491 2.55e-39 146
Msa0530930 MtrunA17_Chr2g0279481 31.469 286 126 4 5 220 213 498 2.96e-39 146
Msa0530930 MtrunA17_Chr4g0010091 36.444 225 108 3 25 214 211 435 5.23e-37 139
Msa0530930 MtrunA17_Chr8g0382231 30.328 244 113 6 25 214 215 455 1.86e-32 127
Msa0530930 MtrunA17_Chr1g0195911 32.645 242 120 6 12 214 201 438 1.25e-31 124
Msa0530930 MtrunA17_Chr2g0303541 31.120 241 123 4 17 214 207 447 1.86e-30 121
Msa0530930 MtrunA17_Chr5g0412591 28.881 277 134 5 25 238 181 457 1.61e-29 118
Msa0530930 MtrunA17_Chr7g0268781 31.967 244 120 7 12 214 204 442 2.88e-29 117
Msa0530930 MtrunA17_Chr5g0401451 30.453 243 140 4 25 238 219 461 1.13e-28 115
Msa0530930 MtrunA17_Chr1g0191711 28.000 225 145 4 12 220 208 431 8.66e-28 113
Msa0530930 MtrunA17_Chr2g0285511 28.807 243 134 3 17 220 206 448 1.23e-27 112
Msa0530930 MtrunA17_Chr8g0374011 30.932 236 121 4 21 214 223 458 3.73e-27 111
Msa0530930 MtrunA17_Chr7g0245201 32.328 232 116 6 23 214 231 461 4.57e-27 111
Msa0530930 MtrunA17_Chr1g0181181 27.459 244 133 3 21 220 186 429 6.06e-27 110
Msa0530930 MtrunA17_Chr1g0150191 34.685 222 111 7 25 214 213 432 9.30e-26 107
Msa0530930 MtrunA17_Chr1g0199631 31.004 229 117 4 25 214 176 402 7.62e-25 104
Msa0530930 MtrunA17_Chr4g0053841 32.258 217 118 4 25 214 195 409 1.03e-24 104
Msa0530930 MtrunA17_Chr2g0320691 29.741 232 124 3 22 214 209 440 4.53e-24 102
Msa0530930 MtrunA17_Chr1g0207461 31.278 227 124 5 19 214 207 432 9.80e-23 98.6
Msa0530930 MtrunA17_Chr3g0108331 28.452 239 133 5 9 214 171 404 2.05e-22 97.4
Msa0530930 MtrunA17_Chr5g0401341 32.579 221 113 5 25 214 212 427 2.15e-22 97.8
Msa0530930 MtrunA17_Chr8g0367891 27.451 255 143 5 25 238 199 452 1.04e-21 95.5
Msa0530930 MtrunA17_Chr4g0076521 28.767 219 127 5 25 214 201 419 2.96e-19 88.2
Msa0530930 MtrunA17_Chr5g0432861 25.101 247 140 5 9 214 172 414 6.47e-18 84.3
Msa0530930 MtrunA17_Chr4g0037661 36.029 136 82 2 82 214 277 410 5.82e-16 78.6
Msa0530930 MtrunA17_Chr2g0290741 24.138 319 190 7 25 313 201 497 1.74e-14 73.9
Msa0530930 MtrunA17_Chr4g0023801 27.136 199 134 6 18 208 185 380 4.10e-13 69.7
Msa0530930 MtrunA17_Chr1g0163311 29.082 196 131 5 25 214 210 403 4.86e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0530930 AT1G52770.1 59.514 247 67 4 1 214 1 247 1.53e-95 290
Msa0530930 AT3G15570.1 58.974 234 62 3 25 224 29 262 2.50e-88 271
Msa0530930 AT5G48800.1 38.278 209 109 3 25 214 221 428 1.09e-36 139
Msa0530930 AT5G03250.2 34.043 235 115 5 25 220 107 340 2.45e-31 122
Msa0530930 AT5G03250.1 34.043 235 115 5 25 220 219 452 9.12e-31 122
Msa0530930 AT1G30440.1 31.020 245 122 3 17 214 208 452 7.77e-30 119
Msa0530930 AT5G64330.1 37.017 181 80 1 74 220 375 555 2.02e-28 115
Msa0530930 AT5G64330.1 46.032 63 29 1 5 67 237 294 1.95e-12 68.6
Msa0530930 AT5G64330.3 37.017 181 80 1 74 220 316 496 2.28e-28 115
Msa0530930 AT5G64330.3 46.032 63 29 1 5 67 178 235 1.79e-12 68.2
Msa0530930 AT5G64330.2 37.017 181 80 1 74 220 375 555 3.07e-28 115
Msa0530930 AT5G64330.2 46.032 63 29 1 5 67 237 294 2.01e-12 68.2
Msa0530930 AT3G44820.1 31.837 245 114 4 22 214 210 453 2.16e-27 112
Msa0530930 AT1G03010.2 28.906 256 127 6 10 214 169 420 3.60e-27 112
Msa0530930 AT1G03010.1 28.906 256 127 6 10 214 196 447 4.30e-27 111
Msa0530930 AT1G03010.3 28.906 256 127 6 10 214 195 446 5.55e-27 111
Msa0530930 AT2G47860.5 33.761 234 108 6 25 214 90 320 7.12e-27 110
Msa0530930 AT2G47860.2 33.761 234 108 6 25 214 90 320 7.12e-27 110
Msa0530930 AT2G47860.3 33.761 234 108 6 25 214 231 461 7.71e-27 111
Msa0530930 AT2G47860.1 33.761 234 108 6 25 214 208 438 9.31e-27 110
Msa0530930 AT3G26490.2 31.985 272 126 8 25 244 220 484 1.08e-26 110
Msa0530930 AT2G47860.6 33.761 234 108 6 25 214 231 461 2.44e-26 108
Msa0530930 AT2G47860.4 33.761 234 108 6 25 214 208 438 2.79e-26 108
Msa0530930 AT5G67385.4 28.333 240 146 3 25 238 158 397 2.79e-26 109
Msa0530930 AT5G67385.5 28.333 240 146 3 25 238 158 397 2.79e-26 109
Msa0530930 AT5G67385.2 28.333 240 146 3 25 238 158 397 2.79e-26 109
Msa0530930 AT5G67385.3 28.333 240 146 3 25 238 158 397 2.79e-26 109
Msa0530930 AT5G67385.1 28.333 240 146 3 25 238 203 442 3.23e-26 109
Msa0530930 AT5G13600.1 30.702 228 120 4 25 214 210 437 5.65e-26 108
Msa0530930 AT3G26490.1 33.043 230 104 6 25 208 220 445 6.85e-26 108
Msa0530930 AT3G26490.3 33.043 230 104 6 25 208 220 445 6.85e-26 108
Msa0530930 AT3G26490.4 33.043 230 104 6 25 208 220 445 6.85e-26 108
Msa0530930 AT2G30520.1 31.489 235 122 5 15 214 183 413 8.89e-26 107
Msa0530930 AT5G66560.4 32.900 231 123 5 15 214 206 435 1.07e-25 107
Msa0530930 AT5G66560.1 32.900 231 123 5 15 214 245 474 1.07e-25 107
Msa0530930 AT2G30520.2 31.489 235 122 5 15 214 135 365 1.12e-25 107
Msa0530930 AT2G30520.3 31.489 235 122 5 15 214 135 365 1.12e-25 107
Msa0530930 AT5G66560.3 32.900 231 123 5 15 214 216 445 1.13e-25 107
Msa0530930 AT5G66560.2 32.627 236 127 5 15 219 245 479 1.56e-25 106
Msa0530930 AT3G08660.3 31.481 216 123 6 23 214 136 350 1.32e-24 104
Msa0530930 AT3G08660.1 31.481 216 123 6 23 214 196 410 1.96e-24 103
Msa0530930 AT3G08660.2 31.222 221 127 6 23 219 196 415 6.76e-24 101
Msa0530930 AT2G14820.2 31.111 225 119 5 25 214 209 432 6.57e-23 99.8
Msa0530930 AT2G14820.1 31.111 225 119 5 25 214 209 432 6.57e-23 99.8
Msa0530930 AT1G67900.5 27.536 276 138 4 25 238 202 477 1.07e-22 99.0
Msa0530930 AT1G67900.1 27.536 276 138 4 25 238 202 477 1.07e-22 99.0
Msa0530930 AT1G67900.3 27.536 276 138 4 25 238 202 477 1.07e-22 99.0
Msa0530930 AT1G67900.6 27.536 276 138 4 25 238 202 477 1.07e-22 99.0
Msa0530930 AT1G67900.2 27.536 276 138 4 25 238 202 477 1.07e-22 99.0
Msa0530930 AT1G67900.4 27.536 276 138 4 25 238 202 477 1.07e-22 99.0
Msa0530930 AT5G10250.2 26.122 245 137 3 14 214 202 446 1.57e-22 98.6
Msa0530930 AT5G10250.1 25.957 235 135 3 14 214 202 431 1.34e-21 95.5
Msa0530930 AT3G50840.2 30.374 214 125 3 22 214 157 367 2.37e-21 94.7
Msa0530930 AT3G50840.3 30.374 214 125 3 22 214 157 367 2.37e-21 94.7
Msa0530930 AT3G08570.1 29.114 237 127 6 21 220 208 440 2.50e-21 94.7
Msa0530930 AT3G50840.1 30.374 214 125 3 22 214 193 403 2.69e-21 94.7
Msa0530930 AT4G31820.2 27.731 238 128 7 1 214 142 359 3.39e-21 94.4
Msa0530930 AT3G49970.1 30.808 198 119 4 25 204 151 348 3.42e-21 94.4
Msa0530930 AT3G49970.2 30.808 198 119 4 25 204 151 348 3.42e-21 94.4
Msa0530930 AT4G31820.1 27.731 238 128 7 1 214 195 412 4.56e-21 94.0
Msa0530930 AT2G23050.2 30.846 201 113 5 25 214 93 278 1.47e-20 91.3
Msa0530930 AT2G23050.1 30.846 201 113 5 25 214 209 394 2.88e-20 91.3
Msa0530930 AT5G47800.3 29.730 222 122 4 25 214 201 420 5.07e-20 90.9
Msa0530930 AT5G47800.1 29.730 222 122 4 25 214 201 420 5.07e-20 90.9
Msa0530930 AT5G47800.4 29.730 222 122 4 25 214 201 420 5.07e-20 90.9
Msa0530930 AT5G47800.2 29.730 222 122 4 25 214 314 533 5.38e-20 90.9
Msa0530930 AT5G67440.2 28.372 215 128 5 25 214 170 383 2.86e-19 88.6
Msa0530930 AT5G67440.3 28.372 215 128 5 25 214 207 420 4.39e-19 88.2
Msa0530930 AT5G67440.1 28.372 215 128 5 25 214 207 420 4.39e-19 88.2
Msa0530930 AT4G37590.1 28.571 245 137 7 15 238 206 433 3.53e-18 85.5
Msa0530930 AT3G19850.1 28.293 205 136 3 21 214 205 409 4.32e-16 79.3
Msa0530930 AT3G22104.4 29.592 196 127 6 25 214 114 304 1.15e-15 77.8
Msa0530930 AT3G22104.2 29.592 196 127 6 25 214 114 304 1.15e-15 77.8
Msa0530930 AT3G22104.3 29.592 196 127 6 25 214 114 304 1.15e-15 77.8
Msa0530930 AT3G22104.1 29.592 196 127 6 25 214 189 379 1.61e-15 77.4

Find 96 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCAATAAATTAATGTATT+TGG 0.109853 4_1:+5029745 Msa0530930:three_prime_UTR
TACTTTGTGAAAACACTTTC+TGG 0.149021 4_1:+5027256 Msa0530930:CDS
ATATGCGAAACTCTTGTATT+TGG 0.157617 4_1:+5029717 Msa0530930:three_prime_UTR
GAGAAGTTTGATGGTGATTT+TGG 0.203132 4_1:-5027190 None:intergenic
TCACACTTGTGGGACTCTTT+TGG 0.252553 4_1:+5027618 Msa0530930:CDS
GATGAGTTTGTTACTCTTGC+TGG 0.253315 4_1:+5027784 Msa0530930:CDS
ACTTCTTGTGATGCTCATTA+TGG 0.274016 4_1:-5029000 None:intergenic
ATAGATTTCCTCCACCTATT+AGG 0.281581 4_1:-5029368 None:intergenic
GTAATTCGTAACTGCAATTA+TGG 0.286116 4_1:-5029489 None:intergenic
GGATGCATAGTGTGTGATTA+TGG 0.305856 4_1:-5027317 None:intergenic
GGTGTTCGAGCCGACTTAAT+CGG 0.338282 4_1:+5027292 Msa0530930:CDS
CTTTCTTGCTTTGATACACT+TGG 0.344184 4_1:-5028810 None:intergenic
CATGGAATTGAATCTTTCTC+TGG 0.346759 4_1:-5027460 None:intergenic
AGAAGAGGAGAAGGTGTTGA+TGG 0.353384 4_1:+5029260 Msa0530930:three_prime_UTR
AGAAGTCCAACAAATGGATT+TGG 0.363197 4_1:+5029017 Msa0530930:CDS
ACTTGTATTACTGCTCTTAC+CGG 0.366047 4_1:-5028912 None:intergenic
ATCGCGATCCTTGATATTGC+AGG 0.367982 4_1:+5029447 Msa0530930:three_prime_UTR
AGCACAAAACGAACGATTCC+CGG 0.370478 4_1:+5028893 Msa0530930:CDS
TATGTCTTGAAAATAAAATA+TGG 0.371656 4_1:+5029388 Msa0530930:three_prime_UTR
AGGTTTGGTGTGCATGGTTT+TGG 0.374755 4_1:+5029215 Msa0530930:three_prime_UTR
TCTAGTCCAAATCCATTTGT+TGG 0.382148 4_1:-5029023 None:intergenic
GCTAACATGGTTGGTGTAGA+TGG 0.384501 4_1:+5027511 Msa0530930:CDS
GGAGATGATCAGGCTGAGTT+TGG 0.390818 4_1:+5029281 Msa0530930:three_prime_UTR
TGCTAAAAGTGGTGCTGCTT+TGG 0.397041 4_1:+5027696 Msa0530930:CDS
AAGTGGTGCTGCTTTGGTTA+AGG 0.398353 4_1:+5027702 Msa0530930:CDS
TGGTAAAGGAGAGAGTGTTA+TGG 0.398752 4_1:+5029235 Msa0530930:three_prime_UTR
CATTCTGAGGAGAAGTTTGA+TGG 0.400975 4_1:-5027199 None:intergenic
AATATTTGTTTGTTCTGTGT+TGG 0.408948 4_1:-5027164 None:intergenic
TAGTTAATAAGTTTGTTTGT+TGG 0.410244 4_1:+5029789 Msa0530930:three_prime_UTR
TCAAAGGTAGGACTCCTAAT+AGG 0.421712 4_1:+5029354 Msa0530930:three_prime_UTR
AGAATGGTTGAGAAGAAGAA+AGG 0.437986 4_1:+5029173 Msa0530930:three_prime_UTR
TATGCAGACTAATAGATTGC+AGG 0.439967 4_1:+5028841 Msa0530930:CDS
CATGATGTTGTTACACTCTC+AGG 0.440841 4_1:-5027391 None:intergenic
TTCCATGTAACTTTCTCCTT+AGG 0.442375 4_1:+5027476 Msa0530930:CDS
CTGCTGTTGATGCTAATTTG+AGG 0.442702 4_1:+5027758 Msa0530930:CDS
CTTGATATTGCAGGAAATTG+AGG 0.443895 4_1:+5029456 Msa0530930:three_prime_UTR
TTTGGTTAAGGTTGCTAAGT+TGG 0.446507 4_1:+5027714 Msa0530930:CDS
GAATGGTTGAGAAGAAGAAA+GGG 0.448107 4_1:+5029174 Msa0530930:three_prime_UTR
GGCTACGTCAGGGAGCCATT+TGG 0.450333 4_1:-5027338 None:intergenic
TGTATCAAAGCAAGAAAGAA+AGG 0.456846 4_1:+5028815 Msa0530930:CDS
CTCAATTTCCTGCAATATCA+AGG 0.458059 4_1:-5029455 None:intergenic
TTTGTTAGTTTGGATAATGA+AGG 0.466879 4_1:+5027673 Msa0530930:intron
TAGTTAATAAGTTTGTTTGT+TGG 0.472522 4_1:+5029674 Msa0530930:three_prime_UTR
TTTAAGTGGAGTAAGAGGTT+TGG 0.480562 4_1:+5029200 Msa0530930:three_prime_UTR
AGCTAGAGAAGAGAATTTCA+TGG 0.481388 4_1:+5027548 Msa0530930:CDS
AGGTGTTGATGGAGATGATC+AGG 0.488953 4_1:+5029271 Msa0530930:three_prime_UTR
TTGTTTGTTCTGTGTTGGAA+TGG 0.488967 4_1:-5027159 None:intergenic
AGTAAGAGGTTTGGTGTGCA+TGG 0.489469 4_1:+5029209 Msa0530930:three_prime_UTR
AGATAGAGATAAAGAAGCTA+AGG 0.489951 4_1:+5029096 Msa0530930:CDS
CTACTTCTGATATGAAAACT+AGG 0.495642 4_1:+5029321 Msa0530930:three_prime_UTR
TTGTTATTAGGCTTGTTAAG+AGG 0.498185 4_1:+5027650 Msa0530930:CDS
ATATCAATCACAACATGGAT+TGG 0.500377 4_1:+5028970 Msa0530930:CDS
AGCTAAGGGAAGATGTTCAT+AGG 0.502264 4_1:+5029111 Msa0530930:CDS
CTTTCTGGTATCAAAGCAAA+AGG 0.503593 4_1:+5027271 Msa0530930:CDS
TTCATATCAGAAGTAGTAGC+AGG 0.504839 4_1:-5029314 None:intergenic
GCAAGTTCAGATGGAGAGAA+TGG 0.512626 4_1:+5029157 Msa0530930:three_prime_UTR
GCTTTGTTTGCTCTGAGAGA+AGG 0.520683 4_1:-5028935 None:intergenic
TTCTGATATGAAAACTAGGT+TGG 0.521166 4_1:+5029325 Msa0530930:three_prime_UTR
TGGTGGTAGAACATTAACTA+AGG 0.523325 4_1:-5027440 None:intergenic
ATCACAAGAAGTCCAACAAA+TGG 0.526697 4_1:+5029011 Msa0530930:CDS
TAATACAATGCAAGTTCAGA+TGG 0.527851 4_1:+5029148 Msa0530930:three_prime_UTR
CATGCTAGAGCTACTGATGA+TGG 0.529678 4_1:+5027820 Msa0530930:CDS
TGTTGATGCTAATTTGAGGT+TGG 0.530669 4_1:+5027762 Msa0530930:CDS
GTTATGGAGAGAAGAGGAGA+AGG 0.531293 4_1:+5029251 Msa0530930:three_prime_UTR
TCACACACTATGCATCCAAA+TGG 0.536466 4_1:+5027323 Msa0530930:CDS
CAAACTTCTCCTCAGAATGC+TGG 0.537316 4_1:+5027203 Msa0530930:CDS
TCACAAAGTAGTCCATGTCT+AGG 0.539545 4_1:-5027243 None:intergenic
TTTGATAAACATCGTGCTGT+CGG 0.551346 4_1:-5029053 None:intergenic
TCAGTAGCTCTAGCATGACT+TGG 0.558700 4_1:-5027814 None:intergenic
CCACAAGTGTGACTAAAAGA+AGG 0.559006 4_1:-5027607 None:intergenic
TACGAATTACAATGATGTTG+TGG 0.559598 4_1:+5029502 Msa0530930:three_prime_UTR
CTTGTATTACTGCTCTTACC+GGG 0.560113 4_1:-5028911 None:intergenic
GCGGTGGCTGGTTACACTTA+CGG 0.563535 4_1:+5029420 Msa0530930:three_prime_UTR
GCTATTGAGAACAGCTAACA+TGG 0.564554 4_1:+5027498 Msa0530930:CDS
GAGAGTGTTATGGAGAGAAG+AGG 0.565489 4_1:+5029245 Msa0530930:three_prime_UTR
ACTAGGTTGGTGAATGTCAA+AGG 0.568083 4_1:+5029338 Msa0530930:three_prime_UTR
CAACGACGTAAACACTTCAC+AGG 0.575359 4_1:-5029531 None:intergenic
GTATCAAAGCAAGAAAGAAA+GGG 0.580576 4_1:+5028816 Msa0530930:CDS
TAGCTGTTCTCAATAGCCTA+AGG 0.580728 4_1:-5027492 None:intergenic
TTGTTGGTGTTAGTCTTGTG+AGG 0.585397 4_1:+5029690 Msa0530930:three_prime_UTR
GTAGGACTCCTAATAGGTGG+AGG 0.587068 4_1:+5029360 Msa0530930:three_prime_UTR
GGAATTGAATCTTTCTCTGG+TGG 0.592373 4_1:-5027457 None:intergenic
AAAACGATGAAGTGAAGTGA+AGG 0.593707 4_1:+5029582 Msa0530930:three_prime_UTR
AGCCTAAGGAGAAAGTTACA+TGG 0.596046 4_1:-5027478 None:intergenic
GATAGAGATAAAGAAGCTAA+GGG 0.596543 4_1:+5029097 Msa0530930:CDS
CGACGCATGCATCCTAGACA+TGG 0.605500 4_1:+5027231 Msa0530930:CDS
TTGAGAACAGCTAACATGGT+TGG 0.608458 4_1:+5027502 Msa0530930:CDS
GATAATGAAGGTGCTAAAAG+TGG 0.616365 4_1:+5027685 Msa0530930:CDS
GGTTGGTGAATGTCAAAGGT+AGG 0.618637 4_1:+5029342 Msa0530930:three_prime_UTR
GAAGTGAAGGTTGCATAGCA+TGG 0.624549 4_1:+5029595 Msa0530930:three_prime_UTR
AAGGTAGGACTCCTAATAGG+TGG 0.629028 4_1:+5029357 Msa0530930:three_prime_UTR
AATCACAACATGGATTGGAG+TGG 0.633303 4_1:+5028975 Msa0530930:CDS
CATCAACAGCAGCTTCACAA+AGG 0.652375 4_1:-5027747 None:intergenic
GATTATGGAACCGATTAAGT+CGG 0.654225 4_1:-5027302 None:intergenic
GTCGTCGAACCAGCATTCTG+AGG 0.664519 4_1:-5027212 None:intergenic
ACACAATATCAATCACAACA+TGG 0.716723 4_1:+5028965 Msa0530930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATATATGAAAATATTAG+TGG + chr4_1:5027918-5027937 Msa0530930:intron 10.0%
!! AAAAAAATGCAAAAATAAAA+AGG + chr4_1:5028496-5028515 Msa0530930:intron 10.0%
!!! AATTATTATTGAGTAAATTT+TGG - chr4_1:5028606-5028625 None:intergenic 10.0%
!! ATATTAGTGGTTAATTTATT+AGG + chr4_1:5027931-5027950 Msa0530930:intron 15.0%
!! TATTAGTGGTTAATTTATTA+GGG + chr4_1:5027932-5027951 Msa0530930:intron 15.0%
!! TATGTCTTGAAAATAAAATA+TGG + chr4_1:5029388-5029407 Msa0530930:three_prime_UTR 15.0%
!! ACTCAATAAATTAATGTATT+TGG + chr4_1:5029745-5029764 Msa0530930:three_prime_UTR 15.0%
!!! GATAAAGAAAACATACTTTT+AGG - chr4_1:5027870-5027889 None:intergenic 20.0%
!! ATTAGTGGTTAATTTATTAG+GGG + chr4_1:5027933-5027952 Msa0530930:intron 20.0%
!! ATAAGAATATTCAAACAAAC+AGG + chr4_1:5028278-5028297 Msa0530930:intron 20.0%
!!! TAAAATATGGTTTTAAACTG+CGG + chr4_1:5029401-5029420 Msa0530930:three_prime_UTR 20.0%
!!! TAGTTAATAAGTTTGTTTGT+TGG + chr4_1:5029674-5029693 Msa0530930:three_prime_UTR 20.0%
!!! TAGTTAATAAGTTTGTTTGT+TGG + chr4_1:5029674-5029693 Msa0530930:three_prime_UTR 20.0%
! AATATTTGTTTGTTCTGTGT+TGG - chr4_1:5027167-5027186 None:intergenic 25.0%
!! AAATTGTGTTATACCCTTTT+CGG - chr4_1:5027362-5027381 None:intergenic 25.0%
!! TTTGTTAGTTTGGATAATGA+AGG + chr4_1:5027673-5027692 Msa0530930:intron 25.0%
!!! TACTTTTAGGTAAGTATCAA+TGG - chr4_1:5027857-5027876 None:intergenic 25.0%
! ATTATTAAGAAGGATGTCTA+AGG - chr4_1:5027990-5028009 None:intergenic 25.0%
! ATGTTGAAGGATTATTAAGA+AGG - chr4_1:5028000-5028019 None:intergenic 25.0%
! ACACATAAATACAAGGATTA+AGG - chr4_1:5028116-5028135 None:intergenic 25.0%
! AATCCTTGTATTTATGTGTT+TGG + chr4_1:5028117-5028136 Msa0530930:intron 25.0%
!! AAATCCAAAAAGCCTATTTT+TGG - chr4_1:5028227-5028246 None:intergenic 25.0%
! TTGTTTGAATATTCTTATGC+TGG - chr4_1:5028276-5028295 None:intergenic 25.0%
! ATATTCAAACAAACAGGAAT+AGG + chr4_1:5028284-5028303 Msa0530930:intron 25.0%
! TATTCAAACAAACAGGAATA+GGG + chr4_1:5028285-5028304 Msa0530930:intron 25.0%
! TGATATAAAATCCCTTGATA+TGG + chr4_1:5028332-5028351 Msa0530930:intron 25.0%
! GATATAAAATCCCTTGATAT+GGG + chr4_1:5028333-5028352 Msa0530930:intron 25.0%
! ATATAAAATCCCTTGATATG+GGG + chr4_1:5028334-5028353 Msa0530930:intron 25.0%
! ATTTCTACAATCATTGTACT+CGG + chr4_1:5028623-5028642 Msa0530930:intron 25.0%
TACTTTGTGAAAACACTTTC+TGG + chr4_1:5027256-5027275 Msa0530930:CDS 30.0%
!!! TGGATTTTGAGCTTGTTATT+AGG + chr4_1:5027638-5027657 Msa0530930:CDS 30.0%
!! TTGTTATTAGGCTTGTTAAG+AGG + chr4_1:5027650-5027669 Msa0530930:CDS 30.0%
!! TGTTAAGAGGTTTGTTAGTT+TGG + chr4_1:5027663-5027682 Msa0530930:intron 30.0%
GTTAATTTATTAGGGGAAGA+GGG + chr4_1:5027940-5027959 Msa0530930:intron 30.0%
TAAGAGAGTTTAAATCTCCT+CGG - chr4_1:5028077-5028096 None:intergenic 30.0%
AGGAGATTTAAACTCTCTTA+AGG + chr4_1:5028077-5028096 Msa0530930:intron 30.0%
TTGCCAAACACATAAATACA+AGG - chr4_1:5028123-5028142 None:intergenic 30.0%
! TTTTGACACTTATAGTACCA+AGG + chr4_1:5028180-5028199 Msa0530930:intron 30.0%
! TCTCTTTGGAAACCAAAAAT+AGG + chr4_1:5028212-5028231 Msa0530930:intron 30.0%
! GAAACCAAAAATAGGCTTTT+TGG + chr4_1:5028220-5028239 Msa0530930:intron 30.0%
ATAAGCTTGTAATCTACATG+TGG + chr4_1:5028367-5028386 Msa0530930:intron 30.0%
! ATTGTGTCTTTTTGTGTAAC+AGG + chr4_1:5028782-5028801 Msa0530930:intron 30.0%
TGTATCAAAGCAAGAAAGAA+AGG + chr4_1:5028815-5028834 Msa0530930:CDS 30.0%
GTATCAAAGCAAGAAAGAAA+GGG + chr4_1:5028816-5028835 Msa0530930:CDS 30.0%
ACACAATATCAATCACAACA+TGG + chr4_1:5028965-5028984 Msa0530930:CDS 30.0%
ATATCAATCACAACATGGAT+TGG + chr4_1:5028970-5028989 Msa0530930:CDS 30.0%
AGATAGAGATAAAGAAGCTA+AGG + chr4_1:5029096-5029115 Msa0530930:CDS 30.0%
GATAGAGATAAAGAAGCTAA+GGG + chr4_1:5029097-5029116 Msa0530930:CDS 30.0%
TAATACAATGCAAGTTCAGA+TGG + chr4_1:5029148-5029167 Msa0530930:three_prime_UTR 30.0%
!!! AGAAGAAAGGGTTTTTTAAG+TGG + chr4_1:5029186-5029205 Msa0530930:three_prime_UTR 30.0%
CTACTTCTGATATGAAAACT+AGG + chr4_1:5029321-5029340 Msa0530930:three_prime_UTR 30.0%
TTCTGATATGAAAACTAGGT+TGG + chr4_1:5029325-5029344 Msa0530930:three_prime_UTR 30.0%
GTAATTCGTAACTGCAATTA+TGG - chr4_1:5029492-5029511 None:intergenic 30.0%
TACGAATTACAATGATGTTG+TGG + chr4_1:5029502-5029521 Msa0530930:three_prime_UTR 30.0%
!! CACTTCATCGTTTTAAATTG+CGG - chr4_1:5029576-5029595 None:intergenic 30.0%
ATATGCGAAACTCTTGTATT+TGG + chr4_1:5029717-5029736 Msa0530930:three_prime_UTR 30.0%
TTGTTTGTTCTGTGTTGGAA+TGG - chr4_1:5027162-5027181 None:intergenic 35.0%
!!! GAGAAGTTTGATGGTGATTT+TGG - chr4_1:5027193-5027212 None:intergenic 35.0%
! CTTTCTGGTATCAAAGCAAA+AGG + chr4_1:5027271-5027290 Msa0530930:CDS 35.0%
GATTATGGAACCGATTAAGT+CGG - chr4_1:5027305-5027324 None:intergenic 35.0%
CAACATCATGGATGAAGAAA+AGG + chr4_1:5027404-5027423 Msa0530930:intron 35.0%
TGGTGGTAGAACATTAACTA+AGG - chr4_1:5027443-5027462 None:intergenic 35.0%
CATGGAATTGAATCTTTCTC+TGG - chr4_1:5027463-5027482 None:intergenic 35.0%
TTCCATGTAACTTTCTCCTT+AGG + chr4_1:5027476-5027495 Msa0530930:CDS 35.0%
AGCTAGAGAAGAGAATTTCA+TGG + chr4_1:5027548-5027567 Msa0530930:CDS 35.0%
! CTTCTTTTAGTCACACTTGT+GGG + chr4_1:5027608-5027627 Msa0530930:CDS 35.0%
!! GATAATGAAGGTGCTAAAAG+TGG + chr4_1:5027685-5027704 Msa0530930:CDS 35.0%
TTTGGTTAAGGTTGCTAAGT+TGG + chr4_1:5027714-5027733 Msa0530930:CDS 35.0%
! TGTTGATGCTAATTTGAGGT+TGG + chr4_1:5027762-5027781 Msa0530930:CDS 35.0%
GGTTAATTTATTAGGGGAAG+AGG + chr4_1:5027939-5027958 Msa0530930:intron 35.0%
ACTCTCTTAAGGACATCTAT+AGG + chr4_1:5028088-5028107 Msa0530930:intron 35.0%
CAAAGAGATTCTAATCTCCT+TGG - chr4_1:5028200-5028219 None:intergenic 35.0%
CAAGGAGATTAGAATCTCTT+TGG + chr4_1:5028198-5028217 Msa0530930:intron 35.0%
! AATAGGCTTTTTGGATTTCC+TGG + chr4_1:5028229-5028248 Msa0530930:intron 35.0%
CAACATATGAAACAGTTTCC+AGG - chr4_1:5028250-5028269 None:intergenic 35.0%
TTAAGTTAGACACCAGAAGT+TGG + chr4_1:5028579-5028598 Msa0530930:intron 35.0%
!! GTAAATTTTGGTCCAACTTC+TGG - chr4_1:5028594-5028613 None:intergenic 35.0%
AAGATTAGATAGCTGAGACT+CGG - chr4_1:5028712-5028731 None:intergenic 35.0%
AGCTATCTAATCTTCATCGA+CGG + chr4_1:5028718-5028737 Msa0530930:intron 35.0%
CTTTCTTGCTTTGATACACT+TGG - chr4_1:5028813-5028832 None:intergenic 35.0%
TATGCAGACTAATAGATTGC+AGG + chr4_1:5028841-5028860 Msa0530930:CDS 35.0%
ACTTGTATTACTGCTCTTAC+CGG - chr4_1:5028915-5028934 None:intergenic 35.0%
ACTTCTTGTGATGCTCATTA+TGG - chr4_1:5029003-5029022 None:intergenic 35.0%
ATCACAAGAAGTCCAACAAA+TGG + chr4_1:5029011-5029030 Msa0530930:CDS 35.0%
! AGAAGTCCAACAAATGGATT+TGG + chr4_1:5029017-5029036 Msa0530930:CDS 35.0%
TCTAGTCCAAATCCATTTGT+TGG - chr4_1:5029026-5029045 None:intergenic 35.0%
TTTGATAAACATCGTGCTGT+CGG - chr4_1:5029056-5029075 None:intergenic 35.0%
AGAATGGTTGAGAAGAAGAA+AGG + chr4_1:5029173-5029192 Msa0530930:three_prime_UTR 35.0%
GAATGGTTGAGAAGAAGAAA+GGG + chr4_1:5029174-5029193 Msa0530930:three_prime_UTR 35.0%
!!! GGTTTTTTAAGTGGAGTAAG+AGG + chr4_1:5029195-5029214 Msa0530930:three_prime_UTR 35.0%
TTTAAGTGGAGTAAGAGGTT+TGG + chr4_1:5029200-5029219 Msa0530930:three_prime_UTR 35.0%
TTCATATCAGAAGTAGTAGC+AGG - chr4_1:5029317-5029336 None:intergenic 35.0%
ATAGATTTCCTCCACCTATT+AGG - chr4_1:5029371-5029390 None:intergenic 35.0%
!!! AATATGGTTTTAAACTGCGG+TGG + chr4_1:5029404-5029423 Msa0530930:three_prime_UTR 35.0%
CTCAATTTCCTGCAATATCA+AGG - chr4_1:5029458-5029477 None:intergenic 35.0%
CTTGATATTGCAGGAAATTG+AGG + chr4_1:5029456-5029475 Msa0530930:three_prime_UTR 35.0%
AAAACGATGAAGTGAAGTGA+AGG + chr4_1:5029582-5029601 Msa0530930:three_prime_UTR 35.0%
! CATTCTGAGGAGAAGTTTGA+TGG - chr4_1:5027202-5027221 None:intergenic 40.0%
TCACAAAGTAGTCCATGTCT+AGG - chr4_1:5027246-5027265 None:intergenic 40.0%
GGATGCATAGTGTGTGATTA+TGG - chr4_1:5027320-5027339 None:intergenic 40.0%
TCACACACTATGCATCCAAA+TGG + chr4_1:5027323-5027342 Msa0530930:CDS 40.0%
CATGATGTTGTTACACTCTC+AGG - chr4_1:5027394-5027413 None:intergenic 40.0%
! CTGAGAGTGTAACAACATCA+TGG + chr4_1:5027392-5027411 Msa0530930:intron 40.0%
GGAATTGAATCTTTCTCTGG+TGG - chr4_1:5027460-5027479 None:intergenic 40.0%
AGCCTAAGGAGAAAGTTACA+TGG - chr4_1:5027481-5027500 None:intergenic 40.0%
TAGCTGTTCTCAATAGCCTA+AGG - chr4_1:5027495-5027514 None:intergenic 40.0%
GCTATTGAGAACAGCTAACA+TGG + chr4_1:5027498-5027517 Msa0530930:CDS 40.0%
TTGAGAACAGCTAACATGGT+TGG + chr4_1:5027502-5027521 Msa0530930:CDS 40.0%
CCACAAGTGTGACTAAAAGA+AGG - chr4_1:5027610-5027629 None:intergenic 40.0%
! CCTTCTTTTAGTCACACTTG+TGG + chr4_1:5027607-5027626 Msa0530930:CDS 40.0%
CTGCTGTTGATGCTAATTTG+AGG + chr4_1:5027758-5027777 Msa0530930:CDS 40.0%
!! GATGAGTTTGTTACTCTTGC+TGG + chr4_1:5027784-5027803 Msa0530930:CDS 40.0%
AGTATGAGTCGAGATGTTGA+AGG - chr4_1:5028013-5028032 None:intergenic 40.0%
CTAGCTAGGCTATAGCTTAA+TGG + chr4_1:5028034-5028053 Msa0530930:intron 40.0%
! TCATTTTACAACTGTCTCCG+AGG + chr4_1:5028057-5028076 Msa0530930:intron 40.0%
! CTAAAGTGTCCCCATATCAA+GGG - chr4_1:5028346-5028365 None:intergenic 40.0%
TAGTCCACATACTCATGCAT+GGG + chr4_1:5028398-5028417 Msa0530930:intron 40.0%
! TTTTCCCATGCATGAGTATG+TGG - chr4_1:5028405-5028424 None:intergenic 40.0%
CTTGTATTACTGCTCTTACC+GGG - chr4_1:5028914-5028933 None:intergenic 40.0%
!! AATCACAACATGGATTGGAG+TGG + chr4_1:5028975-5028994 Msa0530930:CDS 40.0%
AGCTAAGGGAAGATGTTCAT+AGG + chr4_1:5029111-5029130 Msa0530930:CDS 40.0%
!! TGGTAAAGGAGAGAGTGTTA+TGG + chr4_1:5029235-5029254 Msa0530930:three_prime_UTR 40.0%
!! ACTAGGTTGGTGAATGTCAA+AGG + chr4_1:5029338-5029357 Msa0530930:three_prime_UTR 40.0%
TCAAAGGTAGGACTCCTAAT+AGG + chr4_1:5029354-5029373 Msa0530930:three_prime_UTR 40.0%
!! TTGTTGGTGTTAGTCTTGTG+AGG + chr4_1:5029690-5029709 Msa0530930:three_prime_UTR 40.0%
CAAACTTCTCCTCAGAATGC+TGG + chr4_1:5027203-5027222 Msa0530930:CDS 45.0%
! GCTAACATGGTTGGTGTAGA+TGG + chr4_1:5027511-5027530 Msa0530930:CDS 45.0%
! TCACACTTGTGGGACTCTTT+TGG + chr4_1:5027618-5027637 Msa0530930:CDS 45.0%
!! TGCTAAAAGTGGTGCTGCTT+TGG + chr4_1:5027696-5027715 Msa0530930:CDS 45.0%
!! AAGTGGTGCTGCTTTGGTTA+AGG + chr4_1:5027702-5027721 Msa0530930:CDS 45.0%
CATCAACAGCAGCTTCACAA+AGG - chr4_1:5027750-5027769 None:intergenic 45.0%
!! TCAGTAGCTCTAGCATGACT+TGG - chr4_1:5027817-5027836 None:intergenic 45.0%
CATGCTAGAGCTACTGATGA+TGG + chr4_1:5027820-5027839 Msa0530930:CDS 45.0%
CTCGACTCATACTACTAGCT+AGG + chr4_1:5028020-5028039 Msa0530930:intron 45.0%
! CCTAAAGTGTCCCCATATCA+AGG - chr4_1:5028347-5028366 None:intergenic 45.0%
CCTTGATATGGGGACACTTT+AGG + chr4_1:5028344-5028363 Msa0530930:intron 45.0%
CTAGTCCACATACTCATGCA+TGG + chr4_1:5028397-5028416 Msa0530930:intron 45.0%
AGCACAAAACGAACGATTCC+CGG + chr4_1:5028893-5028912 Msa0530930:CDS 45.0%
!! GCTTTGTTTGCTCTGAGAGA+AGG - chr4_1:5028938-5028957 None:intergenic 45.0%
GCAAGTTCAGATGGAGAGAA+TGG + chr4_1:5029157-5029176 Msa0530930:three_prime_UTR 45.0%
!! AGTAAGAGGTTTGGTGTGCA+TGG + chr4_1:5029209-5029228 Msa0530930:three_prime_UTR 45.0%
!!! AGGTTTGGTGTGCATGGTTT+TGG + chr4_1:5029215-5029234 Msa0530930:three_prime_UTR 45.0%
!!! GGTGTGCATGGTTTTGGTAA+AGG + chr4_1:5029221-5029240 Msa0530930:three_prime_UTR 45.0%
!! GAGAGTGTTATGGAGAGAAG+AGG + chr4_1:5029245-5029264 Msa0530930:three_prime_UTR 45.0%
GTTATGGAGAGAAGAGGAGA+AGG + chr4_1:5029251-5029270 Msa0530930:three_prime_UTR 45.0%
!! AGAAGAGGAGAAGGTGTTGA+TGG + chr4_1:5029260-5029279 Msa0530930:three_prime_UTR 45.0%
!! AGGTGTTGATGGAGATGATC+AGG + chr4_1:5029271-5029290 Msa0530930:three_prime_UTR 45.0%
!! GGTTGGTGAATGTCAAAGGT+AGG + chr4_1:5029342-5029361 Msa0530930:three_prime_UTR 45.0%
AAGGTAGGACTCCTAATAGG+TGG + chr4_1:5029357-5029376 Msa0530930:three_prime_UTR 45.0%
ATCGCGATCCTTGATATTGC+AGG + chr4_1:5029447-5029466 Msa0530930:three_prime_UTR 45.0%
CAACGACGTAAACACTTCAC+AGG - chr4_1:5029534-5029553 None:intergenic 45.0%
GAAGTGAAGGTTGCATAGCA+TGG + chr4_1:5029595-5029614 Msa0530930:three_prime_UTR 45.0%
! GGTGTTCGAGCCGACTTAAT+CGG + chr4_1:5027292-5027311 Msa0530930:CDS 50.0%
! TACCCTTTTCGGCTACGTCA+GGG - chr4_1:5027351-5027370 None:intergenic 50.0%
! ATACCCTTTTCGGCTACGTC+AGG - chr4_1:5027352-5027371 None:intergenic 50.0%
GGAGATGATCAGGCTGAGTT+TGG + chr4_1:5029281-5029300 Msa0530930:three_prime_UTR 50.0%
GTAGGACTCCTAATAGGTGG+AGG + chr4_1:5029360-5029379 Msa0530930:three_prime_UTR 50.0%
!!! TGGTTTTAAACTGCGGTGGC+TGG + chr4_1:5029408-5029427 Msa0530930:three_prime_UTR 50.0%
GTCGTCGAACCAGCATTCTG+AGG - chr4_1:5027215-5027234 None:intergenic 55.0%
CGACGCATGCATCCTAGACA+TGG + chr4_1:5027231-5027250 Msa0530930:CDS 55.0%
CTCCCTGACGTAGCCGAAAA+GGG + chr4_1:5027346-5027365 Msa0530930:intron 55.0%
! GCGGTGGCTGGTTACACTTA+CGG + chr4_1:5029420-5029439 Msa0530930:three_prime_UTR 55.0%
GGCTACGTCAGGGAGCCATT+TGG - chr4_1:5027341-5027360 None:intergenic 60.0%
GCTCCCTGACGTAGCCGAAA+AGG + chr4_1:5027345-5027364 Msa0530930:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 5027151 5029854 5027151 ID=Msa0530930;Name=Msa0530930
chr4_1 mRNA 5027151 5029854 5027151 ID=Msa0530930-mRNA-1;Parent=Msa0530930;Name=Msa0530930-mRNA-1;_AED=0.05;_eAED=0.06;_QI=0|0|0.2|1|0|0.2|5|722|317
chr4_1 exon 5027151 5027354 5027151 ID=Msa0530930-mRNA-1:exon:1531;Parent=Msa0530930-mRNA-1
chr4_1 exon 5027386 5027399 5027386 ID=Msa0530930-mRNA-1:exon:1532;Parent=Msa0530930-mRNA-1
chr4_1 exon 5027426 5027671 5027426 ID=Msa0530930-mRNA-1:exon:1533;Parent=Msa0530930-mRNA-1
chr4_1 exon 5027681 5027841 5027681 ID=Msa0530930-mRNA-1:exon:1534;Parent=Msa0530930-mRNA-1
chr4_1 exon 5028804 5029854 5028804 ID=Msa0530930-mRNA-1:exon:1535;Parent=Msa0530930-mRNA-1
chr4_1 CDS 5027151 5027354 5027151 ID=Msa0530930-mRNA-1:cds;Parent=Msa0530930-mRNA-1
chr4_1 CDS 5027386 5027399 5027386 ID=Msa0530930-mRNA-1:cds;Parent=Msa0530930-mRNA-1
chr4_1 CDS 5027426 5027671 5027426 ID=Msa0530930-mRNA-1:cds;Parent=Msa0530930-mRNA-1
chr4_1 CDS 5027681 5027841 5027681 ID=Msa0530930-mRNA-1:cds;Parent=Msa0530930-mRNA-1
chr4_1 CDS 5028804 5029132 5028804 ID=Msa0530930-mRNA-1:cds;Parent=Msa0530930-mRNA-1
chr4_1 three_prime_UTR 5029133 5029854 5029133 ID=Msa0530930-mRNA-1:three_prime_utr;Parent=Msa0530930-mRNA-1
Gene Sequence

>Msa0530930

ATGAAGAACCATTCCAACACAGAACAAACAAATATTCAACCAAAATCACCATCAAACTTCTCCTCAGAATGCTGGTTCGACGACGCATGCATCCTAGACATGGACTACTTTGTGAAAACACTTTCTGGTATCAAAGCAAAAGGTGTTCGAGCCGACTTAATCGGTTCCATAATCACACACTATGCATCCAAATGGCTCCCTGACAATCACCTGAGAGTGTTCTTTGTAGAAACCTTAGTTAATGTTCTACCACCAGAGAAAGATTCAATTCCATGTAACTTTCTCCTTAGGCTATTGAGAACAGCTAACATGGTTGGTGTAGATGGAAGCTATAGACAAGAGCTAGAGAAGAGAATTTCATGGCAGTTAGATCAAGCTTGTTTGAAAGAACTTGTTATTCCTTCTTTTAGTCACACTTGTGGGACTCTTTTGGATTTTGAGCTTGTTATTAGGCTTGTTAAGAGTTTGGATAATGAAGGTGCTAAAAGTGGTGCTGCTTTGGTTAAGGTTGCTAAGTTGGTTGATTCTTACCTTTGTGAAGCTGCTGTTGATGCTAATTTGAGGTTGGATGAGTTTGTTACTCTTGCTGGTGCTCTTCCAAGTCATGCTAGAGCTACTGATGATGGCACATCCAAGTGTATCAAAGCAAGAAAGAAAGGGTCTATGCAGACTAATAGATTGCAGGAAACTAACACAAGAAGCATCACTTCATGCAGCACAAAACGAACGATTCCCGGTAAGAGCAGTAATACAAGTCCTTCTCTCAGAGCAAACAAAGCTCAACAGACACAATATCAATCACAACATGGATTGGAGTGGATCCATAATGAGCATCACAAGAAGTCCAACAAATGGATTTGGACTAGAAGCAGTTCCGACAGCACGATGTTTATCAAAGCGCGAAATGAATGCTCAACAGATAGAGATAAAGAAGCTAAGGGAAGATGTTCATAG
Protein sequence

>Msa0530930

MKNHSNTEQTNIQPKSPSNFSSECWFDDACILDMDYFVKTLSGIKAKGVRADLIGSIITHYASKWLPDNHLRVFFVETLVNVLPPEKDSIPCNFLLRLLRTANMVGVDGSYRQELEKRISWQLDQACLKELVIPSFSHTCGTLLDFELVIRLVKSLDNEGAKSGAALVKVAKLVDSYLCEAAVDANLRLDEFVTLAGALPSHARATDDGTSKCIKARKKGSMQTNRLQETNTRSITSCSTKRTIPGKSSNTSPSLRANKAQQTQYQSQHGLEWIHNEHHKKSNKWIWTRSSSDSTMFIKARNECSTDRDKEAKGRCS*