AlfalfaGEDB | Msa1397540

M. sativa cultivar zhongmu-4 / Msa1397540

KEGG: K08236
GO: -
PFAM: PF00201.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1397540	XP_013470040.1	95.359	474	22	0	1	474	1	474	0.0	921

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1397540	sp\|V5LLZ9\|GGT_QUERO	56.992	472	196	5	1	471	1	466	0.0	575

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1397540	A0A072VRL2	95.359	474	22	0	1	474	1	474	0.0	921

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa1397540	0.854594	1.109018e-61	-8.615850e-47
Msa0000780	Msa1397540	0.800748	1.245715e-48	-8.615850e-47
Msa0001460	Msa1397540	0.845318	4.376197e-59	-8.615850e-47
Msa0003470	Msa1397540	0.847905	8.604713e-60	-8.615850e-47
Msa0004350	Msa1397540	0.852994	3.203389e-61	-8.615850e-47
Msa0004610	Msa1397540	0.820371	7.107331e-53	-8.615850e-47
Msa0005160	Msa1397540	0.803845	2.870291e-49	-8.615850e-47
Msa0005690	Msa1397540	0.856414	3.269327e-62	-8.615850e-47
Msa0006700	Msa1397540	0.884860	1.336607e-71	-8.615850e-47
Msa0006820	Msa1397540	0.850857	1.294430e-60	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1397540	MtrunA17_Chr1g0207861	95.359	474	22	0	1	474	1	474	921
Msa1397540	MtrunA17_Chr1g0161351	86.624	471	61	1	1	469	28	498	832
Msa1397540	MtrunA17_Chr3g0098111	82.636	478	83	0	2	479	3	480	825
Msa1397540	MtrunA17_Chr8g0365811	76.110	473	107	3	3	471	490	960	735
Msa1397540	MtrunA17_Chr8g0365811	75.941	478	108	4	1	471	1	478	719
Msa1397540	MtrunA17_Chr1g0207931	75.523	478	62	3	1	478	85	507	728
Msa1397540	MtrunA17_Chr1g0207951	70.502	478	133	1	2	479	650	1119	692
Msa1397540	MtrunA17_Chr1g0207951	72.257	483	110	7	2	479	118	581	692
Msa1397540	MtrunA17_Chr1g0207971	72.939	473	105	8	1	469	1	454	676
Msa1397540	MtrunA17_Chr1g0207831	68.973	477	137	4	1	474	1	469	663

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1397540	AT4G15480.1	54.095	464	206	6	8	468	19	478	3.16e-180	514
Msa1397540	AT3G21560.1	51.899	474	202	10	8	468	12	472	8.55e-169	485
Msa1397540	AT4G15500.1	50.943	477	209	10	8	475	9	469	1.05e-163	471
Msa1397540	AT4G15490.1	48.745	478	229	9	1	471	1	469	6.54e-162	467
Msa1397540	AT2G23260.1	37.681	483	256	16	1	468	1	453	3.34e-95	295
Msa1397540	AT1G05680.1	34.904	467	274	12	7	465	5	449	6.99e-90	281
Msa1397540	AT1G05675.1	34.542	469	273	11	7	465	5	449	8.14e-89	279
Msa1397540	AT2G43820.1	34.100	478	266	13	8	471	7	449	2.63e-88	277
Msa1397540	AT2G23250.1	36.797	462	261	14	11	468	1	435	3.86e-87	274
Msa1397540	AT2G31750.1	34.937	478	277	13	1	468	1	454	6.04e-87	274

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Find 94 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCACCTTCGGAGGAATT+AGG	0.182104	tig0022164:-48242	Msa1397540:CDS
AAAGAACATAATTTGCCAGA+TGG	0.501406	tig0022164:-48215	Msa1397540:CDS
AACCACCTTCGGAGGAATTA+GGG	0.329917	tig0022164:-48241	Msa1397540:CDS
AAGAACATAATTTGCCAGAT+GGG	0.496155	tig0022164:-48214	Msa1397540:CDS
AAGATGAGTGTCCCTAGAAA+TGG	0.398955	tig0022164:+48599	None:intergenic
AAGGATATGAGTGTTTGCTT+CGG	0.536727	tig0022164:+49177	None:intergenic
AAGGGAAACGTACCAGTTTG+TGG	0.480432	tig0022164:+48714	None:intergenic
AAGTGAATGAAATAGCATAT+GGG	0.601672	tig0022164:-48301	Msa1397540:CDS
AATGTTGACATTTCCAGCAT+GGG	0.490713	tig0022164:-48051	Msa1397540:CDS
ACTTTGCTAGTCTAAGAAGA+GGG	0.596564	tig0022164:+49122	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAGAACATCTCAAACTTGT+TGG	-	tig0022164:48037-48056	Msa1397540:CDS	30.0%
CTATAACTATTTCCACAAAC+TGG	-	tig0022164:48225-48244	Msa1397540:CDS	30.0%
CTAAAAGGATGAAGAAAATC+TGG	+	tig0022164:48325-48344	None:intergenic	30.0%
ATTTCATTCACTTGTTCTTG+AGG	+	tig0022164:48643-48662	None:intergenic	30.0%
CAAGTGAATGAAATAGCATA+TGG	-	tig0022164:48649-48668	Msa1397540:CDS	30.0%
AGTCACAAATGCAAAATTCT+TGG	-	tig0022164:48930-48949	Msa1397540:CDS	30.0%
TGAAGAAAAATGCAATGAAG+TGG	-	tig0022164:49073-49092	Msa1397540:CDS	30.0%
TCGGAATCTTGATGAATTTA+TGG	-	tig0022164:49140-49159	Msa1397540:CDS	30.0%
ATAGAACATCTCAAACTTGT+TGG	-	tig0022164:48037-48056	Msa1397540:CDS	30.0%
CTATAACTATTTCCACAAAC+TGG	-	tig0022164:48225-48244	Msa1397540:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0022164	gene	47767	49206	47767	ID=Msa1397540;Name=Msa1397540
tig0022164	mRNA	47767	49206	47767	ID=Msa1397540-mRNA-1;Parent=Msa1397540;Name=Msa1397540-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|479
tig0022164	exon	47767	49206	47767	ID=Msa1397540-mRNA-1:exon:16149;Parent=Msa1397540-mRNA-1
tig0022164	CDS	47767	49206	47767	ID=Msa1397540-mRNA-1:cds;Parent=Msa1397540-mRNA-1

Gene Sequence

>Msa1397540

ATGGGATCCGAAGCAAACACTCATATCCTTCTTATCTCTTATCCAGCACAAGGACACATAAACCCTCTTCTTAGACTAGCAAAGTGTCTTGCTGCAAAGGGTGCTTCTGTCATCTTCATCACAACAGAGAAAGCTGGCAAAGACATAAGAACTGTTAACAACATCATTGAAAAATCACTCACTCCAATTGGTGATGGTTCTCTCACTTTTGAGTTCTTAGATGATGGTTTAGAAGATGATCATCCTCTAAGAGGAAATCTTTTAGGTTACATAGAACATCTCAAACTTGTTGGAAAACCATTTGTTTCTCAAATGATCAAGAATCATGCTGATTCAAACAAACCCTTTTCATGTATCATAAACAATCCTTTTGTTCCATGGGTTTGTGATGTAGCTGATGAACATGGTATTCCTTCTGCTTTGTTATGGATTCAATCCACTGCTGTTTTTGCTGCTTACTATAACTATTTCCACAAACTGGTACGTTTCCCTTCAGAAACAGAACCTTATATTGATGTTCAATTACCTTTTCAAGTTCTTAAATACAATGAAATCCCAGATTTTCTTCATCCTTTTAGTCAATTTCCATTTCTAGGGACACTCATCTTAGAACAGTTTAAGAATTTGTCTAAAGTATTTTGTGTTTTAGTAGAAACCTATGAAGAACTAGAGCATGATTTCATTGACTATATTTCAAACAAATCAATCTTCGTAAGACCCATTGGTCCATTGTTTCACAATCCAAATATTAAAGGTGCAAAAAATATTCGTGGAGATTTTGTTAAGAGTGATGATTGTAAAATTATAGAGTGGCTAAACACAAAGCCAAAAGGTTCTGTGGTTTATATTTCTTTTGGTACTGTTGTGTACCTTCCTCAAGAACAAGTGAATGAAATAGCATATGGGTTATTGGATTCTCAAGTATCGTTTTTGTGGGTATTAAAACCACCTTCGGAGGAATTAGGGCTAAAAGAACATAATTTGCCAGATGGGTTCTTAGAGGAAACAAGTGAGAGAGGAAAAGTTGTGAAATGGAGTCCACAAGAAGAAGTATTGGCTCATCCATCAGTGGCATGTTTCGTAACACATTGTGGTTGGAATTCATCAATGGAAGCACTTACTTTGGGAGTTCCAATGTTGACATTTCCAGCATGGGGTGATCAAGTCACAAATGCAAAATTCTTGGTAGATGTTTTTGGTGTAGGGATTAGATTGGGTTATGGTATGATTGAAAATAAATTAGTAACAAGAGATGAGGTGAAAAAGTGCTTATTGGAAGCTACAACAGGGGAAAAAGCAGAGGAGTTGAAGAAAAATGCAATGAAGTGGAAGAAAGCGGCAGAGGATGCGGTTGCCGTAGGTGGATCCTCTGATCGGAATCTTGATGAATTTATGGAAGACATTCAGAACCGTGGCACTGTTAATATTCCGAAGATGTAA

Protein sequence

>Msa1397540

MGSEANTHILLISYPAQGHINPLLRLAKCLAAKGASVIFITTEKAGKDIRTVNNIIEKSLTPIGDGSLTFEFLDDGLEDDHPLRGNLLGYIEHLKLVGKPFVSQMIKNHADSNKPFSCIINNPFVPWVCDVADEHGIPSALLWIQSTAVFAAYYNYFHKLVRFPSETEPYIDVQLPFQVLKYNEIPDFLHPFSQFPFLGTLILEQFKNLSKVFCVLVETYEELEHDFIDYISNKSIFVRPIGPLFHNPNIKGAKNIRGDFVKSDDCKIIEWLNTKPKGSVVYISFGTVVYLPQEQVNEIAYGLLDSQVSFLWVLKPPSEELGLKEHNLPDGFLEETSERGKVVKWSPQEEVLAHPSVACFVTHCGWNSSMEALTLGVPMLTFPAWGDQVTNAKFLVDVFGVGIRLGYGMIENKLVTRDEVKKCLLEATTGEKAEELKKNAMKWKKAAEDAVAVGGSSDRNLDEFMEDIQNRGTVNIPKM*