AlfalfaGEDB | Msa0225760

M. sativa cultivar zhongmu-4 / Msa0225760

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225760	XP_003597798.2	91.041	413	33	3	1	412	1	410	0.0	719

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225760	sp\|Q5KQI4\|Y5814_ORYSJ	45.522	268	134	6	16	280	18	276	1.09e-65	214

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225760	G7IMD4	91.041	413	33	3	1	412	1	410	0.0	719

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TFs/TRs:

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Gene ID	Type	Classification
Msa0225760	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003700	Msa0225760	0.805730	1.159359e-49	-8.615850e-47
Msa0004640	Msa0225760	0.806867	6.676221e-50	-8.615850e-47
Msa0007480	Msa0225760	0.818190	2.231163e-52	-8.615850e-47
Msa0008670	Msa0225760	0.817487	3.215034e-52	-8.615850e-47
Msa0012080	Msa0225760	0.815899	7.298117e-52	-8.615850e-47
Msa0016670	Msa0225760	0.814454	1.528047e-51	-8.615850e-47
Msa0017510	Msa0225760	0.860996	1.398984e-63	-8.615850e-47
Msa0028420	Msa0225760	0.828557	8.412307e-55	-8.615850e-47
Msa0028640	Msa0225760	0.861458	1.011790e-63	-8.615850e-47
Msa0029270	Msa0225760	0.810313	1.226289e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225760	MtrunA17_Chr2g0332121	91.041	413	33	3	1	412	1	410	0.0	719
Msa0225760	MtrunA17_Chr4g0002411	76.129	155	37	0	82	236	18	172	1.65e-70	219
Msa0225760	MtrunA17_Chr3g0133791	41.485	229	128	2	9	237	5	227	4.98e-48	163
Msa0225760	MtrunA17_Chr8g0375001	57.143	105	45	0	130	234	34	138	4.08e-38	135

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225760	AT5G58280.3	46.875	256	104	4	12	240	5	255	7.67e-66	213
Msa0225760	AT5G58280.2	46.484	256	106	4	12	240	5	256	9.19e-65	210
Msa0225760	AT5G58280.1	46.522	230	111	3	12	240	5	223	4.68e-59	194
Msa0225760	AT3G19184.1	43.496	246	125	4	12	255	8	241	1.94e-48	166
Msa0225760	AT3G19184.2	43.496	246	125	4	12	255	8	241	2.12e-48	166
Msa0225760	AT5G42700.1	41.228	228	117	4	10	234	7	220	1.12e-44	155
Msa0225760	AT5G42700.2	53.333	120	55	1	116	234	56	175	2.47e-36	131

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Find 89 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAGAAATCGATGCTT+TGG	0.405663	2_1:-75895445	Msa0225760:CDS
AAAAGGGTTTGAATATGAAG+AGG	0.616077	2_1:+75899072	None:intergenic
AAAATGGGCTTAGTGGTGGC+TGG	0.515469	2_1:-75896696	Msa0225760:CDS
AAAGAAAACTAGAATGCAAA+AGG	0.490045	2_1:-75895352	Msa0225760:CDS
AAAGGGGCAATAGTGTCTTT+TGG	0.454116	2_1:+75899098	None:intergenic
AACAGCTATAAACATGAGAC+CGG	0.574078	2_1:+75896980	None:intergenic
AACATTAAACTCTTATTTAG+TGG	0.358356	2_1:-75894776	Msa0225760:three_prime_UTR
AAGAAAACGTGCTTGGGATG+CGG	0.574526	2_1:-75897061	Msa0225760:CDS
AAGAAAAGGAAGGCAAAAGA+AGG	0.525873	2_1:-75895147	Msa0225760:CDS
AAGCAATATCCCTCATAACA+AGG	0.563428	2_1:+75894841	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGGGTTTGAATATGAAG+AGG	+	chr2_1:75894746-75894765	None:intergenic	30.0%
TTGAAAAGATTGAGCTGAAA+AGG	+	chr2_1:75894775-75894794	None:intergenic	30.0%
AAAGTTTCAAGCCATGATTA+AGG	+	chr2_1:75895220-75895239	None:intergenic	30.0%
AAAGAAATAGTTGTCAGATG+TGG	-	chr2_1:75895329-75895348	Msa0225760:CDS	30.0%
AAAATTCAAGACTTCTGTGT+TGG	-	chr2_1:75895494-75895513	Msa0225760:intron	30.0%
CATTGAAATAGAATCCAACT+AGG	+	chr2_1:75895791-75895810	None:intergenic	30.0%
TAAAACTATACTAGTGGTGA+GGG	+	chr2_1:75895872-75895891	None:intergenic	30.0%
TAACGCTAAAACTATACTAG+TGG	+	chr2_1:75895878-75895897	None:intergenic	30.0%
TAAATCCTTGAGAACAAAAG+GGG	+	chr2_1:75895942-75895961	None:intergenic	30.0%
CTAAATCCTTGAGAACAAAA+GGG	+	chr2_1:75895943-75895962	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	75894713	75899124	75894713	ID=Msa0225760;Name=Msa0225760
chr2_1	mRNA	75894713	75899124	75894713	ID=Msa0225760-mRNA-1;Parent=Msa0225760;Name=Msa0225760-mRNA-1;_AED=0.10;_eAED=0.10;_QI=103\|1\|1\|1\|0.71\|0.87\|8\|244\|412
chr2_1	exon	75894713	75895511	75894713	ID=Msa0225760-mRNA-1:exon:22900;Parent=Msa0225760-mRNA-1
chr2_1	exon	75896618	75896824	75896618	ID=Msa0225760-mRNA-1:exon:22899;Parent=Msa0225760-mRNA-1
chr2_1	exon	75896972	75897137	75896972	ID=Msa0225760-mRNA-1:exon:22898;Parent=Msa0225760-mRNA-1
chr2_1	exon	75897216	75897280	75897216	ID=Msa0225760-mRNA-1:exon:22897;Parent=Msa0225760-mRNA-1
chr2_1	exon	75897635	75897730	75897635	ID=Msa0225760-mRNA-1:exon:22896;Parent=Msa0225760-mRNA-1
chr2_1	exon	75897816	75897881	75897816	ID=Msa0225760-mRNA-1:exon:22895;Parent=Msa0225760-mRNA-1
chr2_1	exon	75898673	75898756	75898673	ID=Msa0225760-mRNA-1:exon:22894;Parent=Msa0225760-mRNA-1
chr2_1	exon	75899022	75899124	75899022	ID=Msa0225760-mRNA-1:exon:22893;Parent=Msa0225760-mRNA-1
chr2_1	five_prime_UTR	75899022	75899124	75899022	ID=Msa0225760-mRNA-1:five_prime_utr;Parent=Msa0225760-mRNA-1
chr2_1	CDS	75898673	75898756	75898673	ID=Msa0225760-mRNA-1:cds;Parent=Msa0225760-mRNA-1
chr2_1	CDS	75897816	75897881	75897816	ID=Msa0225760-mRNA-1:cds;Parent=Msa0225760-mRNA-1
chr2_1	CDS	75897635	75897730	75897635	ID=Msa0225760-mRNA-1:cds;Parent=Msa0225760-mRNA-1
chr2_1	CDS	75897216	75897280	75897216	ID=Msa0225760-mRNA-1:cds;Parent=Msa0225760-mRNA-1
chr2_1	CDS	75896972	75897137	75896972	ID=Msa0225760-mRNA-1:cds;Parent=Msa0225760-mRNA-1
chr2_1	CDS	75896618	75896824	75896618	ID=Msa0225760-mRNA-1:cds;Parent=Msa0225760-mRNA-1
chr2_1	CDS	75894957	75895511	75894957	ID=Msa0225760-mRNA-1:cds;Parent=Msa0225760-mRNA-1
chr2_1	three_prime_UTR	75894713	75894956	75894713	ID=Msa0225760-mRNA-1:three_prime_utr;Parent=Msa0225760-mRNA-1

Gene Sequence

>Msa0225760

ATGGCAAAGGGTTGTTCCACTACCAGTAACAAGTATGAAGCGGCCAGAGAACAAAGACTTGAGGAAAACAAGAAGAGGTTTGAGGATTTAGGAATTTCAAAAATTTCCAAAACATTGACAGAAATTGCAAGTCCAGCAAAGAAATCTACGAATCGTCGTTTCAAACCAAAATCAATGACTAATCTTGTTCTGGAACCTAGGCGCTCTTCTCGTGCCAGAAACACAGTTCCTTCGTATGCTGAAGAGTTTGGCACAGACCTTCCACAATTGCAGAGGAGGTCAAGGTCAAGGTCAAGTTCGTCATGGGGAAGTTACGCTTACGCTGCGAGGCCACTGGATGAAATCAAAGTAGCTACAGCTCAAGAAAGAAAACGTGCTTGGGATGCGGCAGAGGCACTCCAATTCAATTTGCAGTCTTCGAATCCATCATTTATCAAGTCAATGCTCCGGTCTCATGTTTATAGCTGTTTTTGGTTGGGTCTTCCCAGTAGATTTTGTGAGGAACATCTTCCAAAGACAGATGATAAATTTGTTTTAGAGGATGAAAAAGGCGCAGAATATGATGCTGTTTACCTTGCCCATAAAAATGGGCTTAGTGGTGGCTGGAGAGGATTTGCATTGAAACATAAATTGGATGATGGTGATGCAGTAGTTTTTGAATTGGTTGAAGCAGCAAGATTTAAGGTTTATATTGTTAGAGCATTTGAAAATATAGATGTAGAGGAAGAAAAAGAAGAAATCGATGCTTTGGTGGAAGACGGAAATATGCAAACATCCAAAGCAATCAAAAAATCTAAAACTGTGAAACCGAAGGAAGCTACAAAGAAAACTAGAATGCAAAAGGTATCTAAAGTACTTACACTTGAGCCAAAGTCTGAAACTGTTAAATCAGAGGAAACTTCTAGGAAAACAAGATCTTCTCATAACAAAAAGCAAAAGGTATCTGATGTATCTACTGCAATTGAGCCAAAAGAGGATGCACACCTCGAAACAGTGAAACCAGAGGCAGCTACAAAGTTGCCAAACAAGAAAAGGAAGGCAAAAGAAGGTGATGTATCTGAGAAGATTCTTTTGGAAGATAATGCTAAAGCAATGAAGACTGACTTAGAGAAGAAGGAAGAACCTGTTGAGGATGTTGATGTCGAGCTAAAGATTAAGCTAGATGATAAAGCAAGCAAACCTGCTGCAGAAAAACCTCGGAAAATTCGAGCTCCAAAGTTTTTTAGAAAGAGAGGTTAG

Protein sequence

>Msa0225760

MAKGCSTTSNKYEAAREQRLEENKKRFEDLGISKISKTLTEIASPAKKSTNRRFKPKSMTNLVLEPRRSSRARNTVPSYAEEFGTDLPQLQRRSRSRSSSSWGSYAYAARPLDEIKVATAQERKRAWDAAEALQFNLQSSNPSFIKSMLRSHVYSCFWLGLPSRFCEEHLPKTDDKFVLEDEKGAEYDAVYLAHKNGLSGGWRGFALKHKLDDGDAVVFELVEAARFKVYIVRAFENIDVEEEKEEIDALVEDGNMQTSKAIKKSKTVKPKEATKKTRMQKVSKVLTLEPKSETVKSEETSRKTRSSHNKKQKVSDVSTAIEPKEDAHLETVKPEAATKLPNKKRKAKEGDVSEKILLEDNAKAMKTDLEKKEEPVEDVDVELKIKLDDKASKPAAEKPRKIRAPKFFRKRG*