AlfalfaGEDB | Msa0361080

M. sativa cultivar zhongmu-4 / Msa0361080

KEGG: K17279
GO: -
PFAM: PF03134.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0361080	XP_003617458.1	97.647	85	2	0	1	85	73	157	8.61e-55	177

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0361080	sp\|Q682H0\|HA22F_ARATH	59.302	86	34	1	1	85	73	158	3.44e-32	112

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0361080	B7FNC9	97.647	85	2	0	1	85	73	157	4.11e-55	177

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0081520	Msa0361080	0.816821	4.539416e-52	-8.615850e-47
Msa0134610	Msa0361080	0.801171	1.021060e-48	-8.615850e-47
Msa0173890	Msa0361080	0.818533	1.865713e-52	-8.615850e-47
Msa0195630	Msa0361080	0.800936	1.140450e-48	-8.615850e-47
Msa0206840	Msa0361080	0.810731	9.965217e-51	-8.615850e-47
Msa0258310	Msa0361080	0.818219	2.197787e-52	-8.615850e-47
Msa0267920	Msa0361080	0.806823	6.823004e-50	-8.615850e-47
Msa0326590	Msa0361080	0.802166	6.381934e-49	-8.615850e-47
Msa0361080	Msa0388770	0.846973	1.551483e-59	-8.615850e-47
Msa0361080	Msa0393440	0.809853	1.540724e-50	-8.615850e-47
Msa0361080	Msa0410650	0.803922	2.766667e-49	-8.615850e-47
Msa0361080	Msa0475790	0.808385	3.178473e-50	-8.615850e-47
Msa0361080	Msa0488790	0.805216	1.485601e-49	-8.615850e-47
Msa0361080	Msa0507680	0.811347	7.326470e-51	-8.615850e-47
Msa0361080	Msa0580440	0.804900	1.730435e-49	-8.615850e-47
Msa0361080	Msa0664910	0.800010	1.761085e-48	-8.615850e-47
Msa0361080	Msa0684500	0.812193	4.794624e-51	-8.615850e-47
Msa0361080	Msa0742740	0.830774	2.429334e-55	-8.615850e-47
Msa0361080	Msa0796160	0.866696	2.360029e-65	-8.615850e-47
Msa0361080	Msa0823820	0.825277	5.118002e-54	-8.615850e-47
Msa0361080	Msa0833760	0.847780	9.312022e-60	-8.615850e-47
Msa0361080	Msa0863010	0.824486	7.865393e-54	-8.615850e-47
Msa0361080	Msa0902760	0.816460	5.466936e-52	-8.615850e-47
Msa0361080	Msa0972100	0.809767	1.608202e-50	-8.615850e-47
Msa0361080	Msa1013400	0.812834	3.473409e-51	-8.615850e-47
Msa0361080	Msa1020630	0.854938	8.816847e-62	-8.615850e-47
Msa0361080	Msa1031930	0.858680	6.975006e-63	-8.615850e-47
Msa0361080	Msa1071770	0.845256	4.548918e-59	-8.615850e-47
Msa0361080	Msa1085200	0.805542	1.269632e-49	-8.615850e-47
Msa0361080	Msa1093530	0.818079	2.363333e-52	-8.615850e-47
Msa0361080	Msa1113800	0.847436	1.157553e-59	-8.615850e-47
Msa0361080	Msa1132700	0.844840	5.893126e-59	-8.615850e-47
Msa0361080	Msa1151910	0.801020	1.096592e-48	-8.615850e-47
Msa0361080	Msa1158830	0.828790	7.392690e-55	-8.615850e-47
Msa0361080	Msa1160500	0.843465	1.377758e-58	-8.615850e-47
Msa0361080	Msa1181190	0.813646	2.303830e-51	-8.615850e-47
Msa0361080	Msa1184330	0.826809	2.212143e-54	-8.615850e-47
Msa0361080	Msa1218600	0.801952	7.064266e-49	-8.615850e-47
Msa0361080	Msa1227940	0.808971	2.383066e-50	-8.615850e-47
Msa0361080	Msa1356830	0.805692	1.180860e-49	-8.615850e-47
Msa0361080	Msa1437940	0.804946	1.691977e-49	-8.615850e-47
Msa0361080	Msa1442820	0.801509	8.709292e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0361080	MtrunA17_Chr5g0443431	97.647	85	2	0	1	85	73	157	7.92e-59	177
Msa0361080	MtrunA17_Chr3g0097141	75.581	86	20	1	1	85	73	158	5.25e-46	144
Msa0361080	MtrunA17_Chr4g0060581	38.750	80	47	1	2	81	82	159	7.39e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0361080	AT2G42820.1	59.302	86	34	1	1	85	73	158	3.50e-33	112
Msa0361080	AT1G74520.1	36.145	83	51	1	1	83	78	158	3.65e-11	56.2
Msa0361080	AT1G74520.2	36.145	83	51	1	1	83	78	158	3.65e-11	56.2

Find 18 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGACATACGTCAAGAAATT+TGG	0.280571	3_1:+51286058	Msa0361080:CDS
GTCAAGAAATTTGGTAACTA+TGG	0.297751	3_1:+51286067	Msa0361080:CDS
GAACGTTATATTGATAGATT+TGG	0.323087	3_1:+51286160	Msa0361080:CDS
ATCACTCTCTCAAATGCATC+TGG	0.360770	3_1:-51286184	None:intergenic
TTCTTCTTCCCATGCCAGCT+TGG	0.474007	3_1:-51286373	None:intergenic
TCAATTTAACCAAGCTGGCA+TGG	0.493645	3_1:+51286364	Msa0361080:three_prime_UTR
TGAAGCTTGTGTTCTGCGTA+TGG	0.521277	3_1:+51285984	Msa0361080:CDS
AGAACCTTCTTGTACTCCTC+AGG	0.524082	3_1:-51286103	None:intergenic
CAATTTAACCAAGCTGGCAT+GGG	0.541105	3_1:+51286365	Msa0361080:three_prime_UTR
CAGAACACAAGCTTCATGTA+TGG	0.542978	3_1:-51285977	None:intergenic
TTGGTACTGCCAATGTTCAA+TGG	0.561469	3_1:+51286007	Msa0361080:CDS
GCTTGTGTTCTGCGTATGGT+TGG	0.576715	3_1:+51285988	Msa0361080:CDS
GAGCGTCAATTTAACCAAGC+TGG	0.586351	3_1:+51286359	Msa0361080:three_prime_UTR
TCACTCTCTCAAATGCATCT+GGG	0.601880	3_1:-51286183	None:intergenic
TAAGCTGCTCCATTGAACAT+TGG	0.632735	3_1:-51286016	None:intergenic
TTATCCTGAGGAGTACAAGA+AGG	0.638050	3_1:+51286099	Msa0361080:CDS
ACATATATGAGAATTATGCA+AGG	0.691799	3_1:+51286038	Msa0361080:CDS
TGGAAATTACAATTATCCTG+AGG	0.710511	3_1:+51286087	Msa0361080:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GATATATATATTTATAACTT+TGG	-	chr3_1:51286244-51286263	None:intergenic	10.0%
!!!	CTATTTTCACTTTATATTTC+AGG	+	chr3_1:51286308-51286327	Msa0361080:intron	20.0%
!	ACATATATGAGAATTATGCA+AGG	+	chr3_1:51286038-51286057	Msa0361080:CDS	25.0%
!	GAACGTTATATTGATAGATT+TGG	+	chr3_1:51286160-51286179	Msa0361080:CDS	25.0%
!	GTCAAGAAATTTGGTAACTA+TGG	+	chr3_1:51286067-51286086	Msa0361080:CDS	30.0%
	TGGAAATTACAATTATCCTG+AGG	+	chr3_1:51286087-51286106	Msa0361080:CDS	30.0%
	AGGACATACGTCAAGAAATT+TGG	+	chr3_1:51286058-51286077	Msa0361080:CDS	35.0%
	CAGAACACAAGCTTCATGTA+TGG	-	chr3_1:51285980-51285999	None:intergenic	40.0%
	TTGGTACTGCCAATGTTCAA+TGG	+	chr3_1:51286007-51286026	Msa0361080:CDS	40.0%
	TAAGCTGCTCCATTGAACAT+TGG	-	chr3_1:51286019-51286038	None:intergenic	40.0%
!	TTATCCTGAGGAGTACAAGA+AGG	+	chr3_1:51286099-51286118	Msa0361080:CDS	40.0%
	TCACTCTCTCAAATGCATCT+GGG	-	chr3_1:51286186-51286205	None:intergenic	40.0%
	ATCACTCTCTCAAATGCATC+TGG	-	chr3_1:51286187-51286206	None:intergenic	40.0%
	TCAATTTAACCAAGCTGGCA+TGG	+	chr3_1:51286364-51286383	Msa0361080:three_prime_UTR	40.0%
	CAATTTAACCAAGCTGGCAT+GGG	+	chr3_1:51286365-51286384	Msa0361080:three_prime_UTR	40.0%
	TGAAGCTTGTGTTCTGCGTA+TGG	+	chr3_1:51285984-51286003	Msa0361080:CDS	45.0%
	AGAACCTTCTTGTACTCCTC+AGG	-	chr3_1:51286106-51286125	None:intergenic	45.0%
	GAGCGTCAATTTAACCAAGC+TGG	+	chr3_1:51286359-51286378	Msa0361080:three_prime_UTR	45.0%
	GCTTGTGTTCTGCGTATGGT+TGG	+	chr3_1:51285988-51286007	Msa0361080:CDS	50.0%
	TTCTTCTTCCCATGCCAGCT+TGG	-	chr3_1:51286376-51286395	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_1	gene	51285973	51286445	51285973	ID=Msa0361080;Name=Msa0361080
chr3_1	mRNA	51285973	51286445	51285973	ID=Msa0361080-mRNA-1;Parent=Msa0361080;Name=Msa0361080-mRNA-1;_AED=0.12;_eAED=0.12;_QI=10\|1\|1\|1\|1\|1\|2\|89\|85
chr3_1	exon	51285973	51286213	51285973	ID=Msa0361080-mRNA-1:exon:9091;Parent=Msa0361080-mRNA-1
chr3_1	exon	51286330	51286445	51286330	ID=Msa0361080-mRNA-1:exon:9092;Parent=Msa0361080-mRNA-1
chr3_1	five_prime_UTR	51285973	51285982	51285973	ID=Msa0361080-mRNA-1:five_prime_utr;Parent=Msa0361080-mRNA-1
chr3_1	CDS	51285983	51286213	51285983	ID=Msa0361080-mRNA-1:cds;Parent=Msa0361080-mRNA-1
chr3_1	CDS	51286330	51286356	51286330	ID=Msa0361080-mRNA-1:cds;Parent=Msa0361080-mRNA-1
chr3_1	three_prime_UTR	51286357	51286445	51286357	ID=Msa0361080-mRNA-1:three_prime_utr;Parent=Msa0361080-mRNA-1

Gene Sequence	>Msa0361080 ATGAAGCTTGTGTTCTGCGTATGGTTGGTACTGCCAATGTTCAATGGAGCAGCTTACATATATGAGAATTATGCAAGGACATACGTCAAGAAATTTGGTAACTATGGAAATTACAATTATCCTGAGGAGTACAAGAAGGTTCTTCATATGATGACTTTCGATGCAAGAAAAGCAGTTGAACGTTATATTGATAGATTTGGCCCAGATGCATTTGAGAGAGTGATTAGAGCAGCTGAGAAAGAAGCAAAGAAGCATTGA
Protein sequence	>Msa0361080 MKLVFCVWLVLPMFNGAAYIYENYARTYVKKFGNYGNYNYPEEYKKVLHMMTFDARKAVERYIDRFGPDAFERVIRAAEKEAKKH*