AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0664910


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0664910 MtrunA17_Chr4g0003001 97.596 208 5 0 1 208 58 265 1.30e-155 431
Msa0664910 MtrunA17_Chr2g0333351 60.000 220 65 10 1 208 1 209 4.63e-76 228
Msa0664910 MtrunA17_Chr4g0064971 48.000 150 67 4 1 149 1 140 2.79e-35 125
Msa0664910 MtrunA17_Chr2g0304551 61.290 93 33 2 1 92 1 91 3.05e-31 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0664910 AT1G15760.1 49.545 220 73 10 3 207 5 201 4.69e-59 184
Msa0664910 AT1G80520.1 50.463 216 73 10 1 207 1 191 2.58e-51 164
Msa0664910 AT2G12462.1 30.709 254 123 9 1 207 1 248 7.75e-27 103

Find 44 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATCTCTTGTATATGAGTA+TGG 0.307188 4_4:-4190258 Msa0664910:CDS
CCATGCTTTGGAGAAATTCA+TGG 0.323501 4_4:+4190184 None:intergenic
GCTAGTAGTAGTAGTTATTA+TGG 0.324043 4_4:-4189961 Msa0664910:CDS
AAACTTGCAAGAAAAGAAAA+AGG 0.339805 4_4:-4190126 Msa0664910:CDS
GAGAATTTCTAGCCTGTGTT+TGG 0.345241 4_4:+4190149 None:intergenic
CTATGGAGATTCCCATGCTT+TGG 0.347816 4_4:+4190172 None:intergenic
ATGATCTTTGCCATTGGTCT+TGG 0.351786 4_4:+4190090 None:intergenic
ATCATGATAGCAATGGTTAT+TGG 0.385458 4_4:-4189753 Msa0664910:CDS
CTACTACTAGCCCTTGTTGT+TGG 0.387585 4_4:+4189973 None:intergenic
CAACCATGGATTGGTTCTCT+TGG 0.390859 4_4:-4190302 None:intergenic
ATCTTTGCCATTGGTCTTGG+TGG 0.398986 4_4:+4190093 None:intergenic
CAGCTGCTGTTGAAGATATC+AGG 0.407684 4_4:-4189729 Msa0664910:CDS
TTCTAGCCTGTGTTTGGCTA+TGG 0.411531 4_4:+4190155 None:intergenic
GCTCCCATGATCTTTGCCAT+TGG 0.442339 4_4:+4190084 None:intergenic
GAGAGGCTGTTGCTTACAAA+TGG 0.465042 4_4:-4189874 Msa0664910:CDS
AAGACCAATGGCAAAGATCA+TGG 0.469365 4_4:-4190088 Msa0664910:CDS
GCAGGCATCATTGAAGGACT+AGG 0.479985 4_4:+4189847 None:intergenic
GAAGAGGAATAAGAAGTTGA+AGG 0.494849 4_4:-4189908 Msa0664910:CDS
TAACTACATAATGAGAAAGT+TGG 0.500944 4_4:-4190034 Msa0664910:CDS
TCAACTTACCAACCATGGAT+TGG 0.503114 4_4:-4190311 None:intergenic
GCAAGAGCAGGCATCATTGA+AGG 0.509469 4_4:+4189841 None:intergenic
CATATACAAGAGATGGATCA+AGG 0.528896 4_4:+4190265 None:intergenic
TTTCATGAAGGAAAGATGAA+AGG 0.538904 4_4:-4189781 Msa0664910:CDS
CCATGAATTTCTCCAAAGCA+TGG 0.539592 4_4:-4190184 Msa0664910:CDS
CCATACTCATATACAAGAGA+TGG 0.559337 4_4:+4190258 None:intergenic
AGACCAATGGCAAAGATCAT+GGG 0.562788 4_4:-4190087 Msa0664910:CDS
GAGGAATAAGAAGTTGAAGG+TGG 0.566010 4_4:-4189905 Msa0664910:CDS
CATGAATTTCTCCAAAGCAT+GGG 0.566734 4_4:-4190183 Msa0664910:CDS
TTGTTGTAGTTCCAACAACA+AGG 0.568940 4_4:-4189984 Msa0664910:CDS
CAGGCATCATTGAAGGACTA+GGG 0.582193 4_4:+4189848 None:intergenic
GAGACAACCACCAAGACCAA+TGG 0.584520 4_4:-4190100 Msa0664910:CDS
TCATGAAAATGATAAACCAT+AGG 0.596177 4_4:+4189796 None:intergenic
GGAAGAGTTCTGTTGTGAAG+AGG 0.606689 4_4:-4189924 Msa0664910:CDS
AAACTATCAAGAGCAAGAGC+AGG 0.606845 4_4:+4189829 None:intergenic
CTGCTGTTGAAGATATCAGG+TGG 0.617099 4_4:-4189726 Msa0664910:CDS
GGAGATGATCATGATAGCAA+TGG 0.631701 4_4:-4189760 Msa0664910:CDS
GCTCATTGTGTGCAAAAGTG+AGG 0.635769 4_4:+4190235 None:intergenic
TGCTGTTGAAGATATCAGGT+GGG 0.636800 4_4:-4189725 Msa0664910:CDS
AAGCCAAGAGAACCAATCCA+TGG 0.643677 4_4:+4190299 None:intergenic
ATTATGGAACAAGATCATCA+TGG 0.658028 4_4:-4189945 Msa0664910:CDS
TGAGCTAGAACAAGAAGACA+TGG 0.668504 4_4:-4190217 Msa0664910:CDS
GAATCTCCATAGCCAAACAC+AGG 0.676222 4_4:-4190161 Msa0664910:CDS
TGTTGTAGTTCCAACAACAA+GGG 0.688058 4_4:-4189983 Msa0664910:CDS
TGAAGGTGGATAAACAAGAG+AGG 0.697861 4_4:-4189891 Msa0664910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAACTTGCAAGAAAAGAAAA+AGG - chr4_4:4189864-4189883 Msa0664910:CDS 25.0%
!!! AAAAAGACAAAAAAGTGCTT+AGG - chr4_4:4189933-4189952 Msa0664910:CDS 25.0%
! TAACTACATAATGAGAAAGT+TGG - chr4_4:4189956-4189975 Msa0664910:CDS 25.0%
! TCATGAAAATGATAAACCAT+AGG + chr4_4:4190197-4190216 None:intergenic 25.0%
!! ATGGTTTATCATTTTCATGA+AGG - chr4_4:4190197-4190216 Msa0664910:CDS 25.0%
GCTAGTAGTAGTAGTTATTA+TGG - chr4_4:4190029-4190048 Msa0664910:CDS 30.0%
ATTATGGAACAAGATCATCA+TGG - chr4_4:4190045-4190064 Msa0664910:CDS 30.0%
! TAGTTTTTGTAGTACTCCTA+TGG - chr4_4:4190178-4190197 Msa0664910:CDS 30.0%
TTTCATGAAGGAAAGATGAA+AGG - chr4_4:4190209-4190228 Msa0664910:CDS 30.0%
ATCATGATAGCAATGGTTAT+TGG - chr4_4:4190237-4190256 Msa0664910:CDS 30.0%
CATATACAAGAGATGGATCA+AGG + chr4_4:4189728-4189747 None:intergenic 35.0%
CCATACTCATATACAAGAGA+TGG + chr4_4:4189735-4189754 None:intergenic 35.0%
CCATCTCTTGTATATGAGTA+TGG - chr4_4:4189732-4189751 Msa0664910:CDS 35.0%
CATGAATTTCTCCAAAGCAT+GGG - chr4_4:4189807-4189826 Msa0664910:CDS 35.0%
TTGTTGTAGTTCCAACAACA+AGG - chr4_4:4190006-4190025 Msa0664910:CDS 35.0%
TGTTGTAGTTCCAACAACAA+GGG - chr4_4:4190007-4190026 Msa0664910:CDS 35.0%
GAAGAGGAATAAGAAGTTGA+AGG - chr4_4:4190082-4190101 Msa0664910:CDS 35.0%
TGAGCTAGAACAAGAAGACA+TGG - chr4_4:4189773-4189792 Msa0664910:CDS 40.0%
!! CCATGCTTTGGAGAAATTCA+TGG + chr4_4:4189809-4189828 None:intergenic 40.0%
CCATGAATTTCTCCAAAGCA+TGG - chr4_4:4189806-4189825 Msa0664910:CDS 40.0%
!! GAGAATTTCTAGCCTGTGTT+TGG + chr4_4:4189844-4189863 None:intergenic 40.0%
! ATGATCTTTGCCATTGGTCT+TGG + chr4_4:4189903-4189922 None:intergenic 40.0%
AAGACCAATGGCAAAGATCA+TGG - chr4_4:4189902-4189921 Msa0664910:CDS 40.0%
AGACCAATGGCAAAGATCAT+GGG - chr4_4:4189903-4189922 Msa0664910:CDS 40.0%
GAGGAATAAGAAGTTGAAGG+TGG - chr4_4:4190085-4190104 Msa0664910:CDS 40.0%
TGAAGGTGGATAAACAAGAG+AGG - chr4_4:4190099-4190118 Msa0664910:CDS 40.0%
AAACTATCAAGAGCAAGAGC+AGG + chr4_4:4190164-4190183 None:intergenic 40.0%
GGAGATGATCATGATAGCAA+TGG - chr4_4:4190230-4190249 Msa0664910:CDS 40.0%
TGCTGTTGAAGATATCAGGT+GGG - chr4_4:4190265-4190284 Msa0664910:CDS 40.0%
GCTCATTGTGTGCAAAAGTG+AGG + chr4_4:4189758-4189777 None:intergenic 45.0%
CTATGGAGATTCCCATGCTT+TGG + chr4_4:4189821-4189840 None:intergenic 45.0%
GAATCTCCATAGCCAAACAC+AGG - chr4_4:4189829-4189848 Msa0664910:CDS 45.0%
TTCTAGCCTGTGTTTGGCTA+TGG + chr4_4:4189838-4189857 None:intergenic 45.0%
!! ATCTTTGCCATTGGTCTTGG+TGG + chr4_4:4189900-4189919 None:intergenic 45.0%
! CTACTACTAGCCCTTGTTGT+TGG + chr4_4:4190020-4190039 None:intergenic 45.0%
GGAAGAGTTCTGTTGTGAAG+AGG - chr4_4:4190066-4190085 Msa0664910:CDS 45.0%
! GAGAGGCTGTTGCTTACAAA+TGG - chr4_4:4190116-4190135 Msa0664910:CDS 45.0%
CAGGCATCATTGAAGGACTA+GGG + chr4_4:4190145-4190164 None:intergenic 45.0%
CAGCTGCTGTTGAAGATATC+AGG - chr4_4:4190261-4190280 Msa0664910:CDS 45.0%
CTGCTGTTGAAGATATCAGG+TGG - chr4_4:4190264-4190283 Msa0664910:CDS 45.0%
GAGACAACCACCAAGACCAA+TGG - chr4_4:4189890-4189909 Msa0664910:CDS 50.0%
GCTCCCATGATCTTTGCCAT+TGG + chr4_4:4189909-4189928 None:intergenic 50.0%
GCAGGCATCATTGAAGGACT+AGG + chr4_4:4190146-4190165 None:intergenic 50.0%
GCAAGAGCAGGCATCATTGA+AGG + chr4_4:4190152-4190171 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 4189693 4190319 4189693 ID=Msa0664910;Name=Msa0664910
chr4_4 mRNA 4189693 4190319 4189693 ID=Msa0664910-mRNA-1;Parent=Msa0664910;Name=Msa0664910-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0|-1|0|1|-1|1|1|0|208
chr4_4 exon 4189693 4190319 4189693 ID=Msa0664910-mRNA-1:exon:1413;Parent=Msa0664910-mRNA-1
chr4_4 CDS 4189693 4190319 4189693 ID=Msa0664910-mRNA-1:cds;Parent=Msa0664910-mRNA-1
Gene Sequence

>Msa0664910

ATGGATTGGTTCTCTTGGCTTTCAAAAACAAACCTTGATCCATCTCTTGTATATGAGTATGGCCTCACTTTTGCACACAATGAGCTAGAACAAGAAGACATGGTTTACTTCAACCATGAATTTCTCCAAAGCATGGGAATCTCCATAGCCAAACACAGGCTAGAAATTCTCAAACTTGCAAGAAAAGAAAAAGGAAAGAGACAACCACCAAGACCAATGGCAAAGATCATGGGAGCAATCAAAAAGACAAAAAAGTGCTTAGGTAACTACATAATGAGAAAGTTGGTTACATGTGAAGAATCAAATTCAGCACTTGTTGTAGTTCCAACAACAAGGGCTAGTAGTAGTAGTTATTATGGAACAAGATCATCATGGAAGAGTTCTGTTGTGAAGAGGAATAAGAAGTTGAAGGTGGATAAACAAGAGAGGCTGTTGCTTACAAATGGAAGCCCTAGTCCTTCAATGATGCCTGCTCTTGCTCTTGATAGTTTTTGTAGTACTCCTATGGTTTATCATTTTCATGAAGGAAAGATGAAAGGAGATGATCATGATAGCAATGGTTATTGGTCAGCTGCTGTTGAAGATATCAGGTGGGATACTATGTTCCAAGATCTAAAACCAAACTAA

Protein sequence

>Msa0664910

MDWFSWLSKTNLDPSLVYEYGLTFAHNELEQEDMVYFNHEFLQSMGISIAKHRLEILKLARKEKGKRQPPRPMAKIMGAIKKTKKCLGNYIMRKLVTCEESNSALVVVPTTRASSSSYYGTRSSWKSSVVKRNKKLKVDKQERLLLTNGSPSPSMMPALALDSFCSTPMVYHFHEGKMKGDDHDSNGYWSAAVEDIRWDTMFQDLKPN*