AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0384750


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0384750 MtrunA17_Chr3g0141281 91.860 172 12 1 1 172 1 170 1.10e-107 312
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0384750 AT1G48460.1 57.143 98 35 3 83 179 71 162 1.27e-24 98.6

Find 36 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATATCAGAAACAGAATTT+TGG 0.173325 3_1:+88056654 Msa0384750:CDS
AGTTCTAGTGTTCCTGTTAA+TGG 0.210562 3_1:+88056898 Msa0384750:CDS
GAGCTCTTACTTATGGGTTT+TGG 0.226459 3_1:+88057038 Msa0384750:CDS
AAGAGGAGAGCTCTTACTTA+TGG 0.285229 3_1:+88057031 Msa0384750:CDS
TTCCATCGTCACATCAGTTT+AGG 0.286689 3_1:-88056447 None:intergenic
GTTCTAGTGTTCCTGTTAAT+GGG 0.303684 3_1:+88056899 Msa0384750:CDS
GAACCATTTCATGGTAAATC+AGG 0.312758 3_1:+88056937 Msa0384750:CDS
TCCATCGTCACATCAGTTTA+GGG 0.322289 3_1:-88056446 None:intergenic
CTACATTTGTTGTGAGTAAA+GGG 0.340887 3_1:+88056389 Msa0384750:five_prime_UTR
TTTATTACTGACACGATGTT+TGG 0.357578 3_1:-88056516 None:intergenic
GCTTCTGATTCCAATGTAAA+TGG 0.366214 3_1:+88056850 Msa0384750:CDS
AATGGGTTGGAACCATTTCA+TGG 0.370157 3_1:+88056928 Msa0384750:CDS
CTTTCGCAGTCACAAGCAAC+TGG 0.380671 3_1:+88056615 Msa0384750:intron
GAACCTGATTTACCATGAAA+TGG 0.410530 3_1:-88056940 None:intergenic
ACTACATTTGTTGTGAGTAA+AGG 0.411169 3_1:+88056388 Msa0384750:five_prime_UTR
AGAGGAGAGCTCTTACTTAT+GGG 0.413551 3_1:+88057032 Msa0384750:CDS
GAGAAGTGAAGTGGTTGAGA+GGG 0.440340 3_1:-88056494 None:intergenic
ACTTCCCTTCTTCTACAGAT+TGG 0.459140 3_1:-88056987 None:intergenic
TTTGATTTCAGCATTCAAAC+AGG 0.466305 3_1:+88056765 Msa0384750:intron
TTCCAATGCACCATTTACAT+TGG 0.492222 3_1:-88056860 None:intergenic
GGAGAAGTGAAGTGGTTGAG+AGG 0.505328 3_1:-88056495 None:intergenic
AAAGATGATATGAATGCGAC+AGG 0.518252 3_1:-88057063 None:intergenic
ATTCTGCCACAGTTCTTCGT+TGG 0.519550 3_1:+88057087 Msa0384750:CDS
ATCTGTAGAAGAAGGGAAGT+TGG 0.519792 3_1:+88056990 Msa0384750:CDS
TCAGTTTAGGGTTCAAGAAG+TGG 0.524196 3_1:-88056434 None:intergenic
CTTCGTTGGCACTGTTGTTG+CGG 0.536995 3_1:+88057101 Msa0384750:CDS
TTCTAGTGTTCCTGTTAATG+GGG 0.540217 3_1:+88056900 Msa0384750:CDS
ACACACCAATCTGTAGAAGA+AGG 0.540836 3_1:+88056982 Msa0384750:CDS
TTCCAATGTAAATGGTGCAT+TGG 0.552698 3_1:+88056858 Msa0384750:CDS
GAGCTCTCCTCTTGTTTGAA+CGG 0.557951 3_1:-88057021 None:intergenic
ATCTGCTCCGTTCAAACAAG+AGG 0.588809 3_1:+88057014 Msa0384750:CDS
CACACCAATCTGTAGAAGAA+GGG 0.595884 3_1:+88056983 Msa0384750:CDS
TCTAGTGTTCCTGTTAATGG+GGG 0.617974 3_1:+88056901 Msa0384750:CDS
CGATGTTTGGAGAAGTGAAG+TGG 0.648063 3_1:-88056503 None:intergenic
ACCCTAAACTGATGTGACGA+TGG 0.681521 3_1:+88056445 Msa0384750:exon
ACAGTGCCAACGAAGAACTG+TGG 0.710210 3_1:-88057093 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATGATTTGATTTTAAA+AGG + chr3_1:88057129-88057148 Msa0384750:CDS 10.0%
!!! TTTAATGATTTGATTTTAAA+AGG + chr3_1:88057129-88057148 Msa0384750:CDS 10.0%
!!! ATTTATAACAATTTGTTGAA+TGG - chr3_1:88056738-88056757 None:intergenic 15.0%
!!! ATTTATAACAATTTGTTGAA+TGG - chr3_1:88056738-88056757 None:intergenic 15.0%
!!! AAATGTACGATTTTTGTTTT+TGG + chr3_1:88056693-88056712 Msa0384750:intron 20.0%
!!! AATGTACGATTTTTGTTTTT+GGG + chr3_1:88056694-88056713 Msa0384750:intron 20.0%
!!! ATTAAAGAACCTTCATTTTT+GGG + chr3_1:88056790-88056809 Msa0384750:CDS 20.0%
!!! AAATGTACGATTTTTGTTTT+TGG + chr3_1:88056693-88056712 Msa0384750:intron 20.0%
!!! AATGTACGATTTTTGTTTTT+GGG + chr3_1:88056694-88056713 Msa0384750:intron 20.0%
!!! ATTAAAGAACCTTCATTTTT+GGG + chr3_1:88056790-88056809 Msa0384750:CDS 20.0%
! AAGAAGAAAAAAAGTTAGTG+TGG - chr3_1:88056595-88056614 None:intergenic 25.0%
!! AGATATCAGAAACAGAATTT+TGG + chr3_1:88056654-88056673 Msa0384750:CDS 25.0%
!! GATATCAGAAACAGAATTTT+GGG + chr3_1:88056655-88056674 Msa0384750:CDS 25.0%
!! GATTAAAGAACCTTCATTTT+TGG + chr3_1:88056789-88056808 Msa0384750:CDS 25.0%
!!! TTTTTCAATCCCAAAAATGA+AGG - chr3_1:88056802-88056821 None:intergenic 25.0%
! TGGGATTGAAAAAATTTCAA+AGG + chr3_1:88056809-88056828 Msa0384750:CDS 25.0%
! AAGAAGAAAAAAAGTTAGTG+TGG - chr3_1:88056595-88056614 None:intergenic 25.0%
!! AGATATCAGAAACAGAATTT+TGG + chr3_1:88056654-88056673 Msa0384750:CDS 25.0%
!! GATATCAGAAACAGAATTTT+GGG + chr3_1:88056655-88056674 Msa0384750:CDS 25.0%
!! GATTAAAGAACCTTCATTTT+TGG + chr3_1:88056789-88056808 Msa0384750:CDS 25.0%
!!! TTTTTCAATCCCAAAAATGA+AGG - chr3_1:88056802-88056821 None:intergenic 25.0%
! TGGGATTGAAAAAATTTCAA+AGG + chr3_1:88056809-88056828 Msa0384750:CDS 25.0%
!! ACTACATTTGTTGTGAGTAA+AGG + chr3_1:88056388-88056407 Msa0384750:five_prime_UTR 30.0%
!! CTACATTTGTTGTGAGTAAA+GGG + chr3_1:88056389-88056408 Msa0384750:five_prime_UTR 30.0%
TTTATTACTGACACGATGTT+TGG - chr3_1:88056519-88056538 None:intergenic 30.0%
! TTTGATTTCAGCATTCAAAC+AGG + chr3_1:88056765-88056784 Msa0384750:intron 30.0%
!! ACTACATTTGTTGTGAGTAA+AGG + chr3_1:88056388-88056407 Msa0384750:five_prime_UTR 30.0%
!! CTACATTTGTTGTGAGTAAA+GGG + chr3_1:88056389-88056408 Msa0384750:five_prime_UTR 30.0%
TTTATTACTGACACGATGTT+TGG - chr3_1:88056519-88056538 None:intergenic 30.0%
! TTTGATTTCAGCATTCAAAC+AGG + chr3_1:88056765-88056784 Msa0384750:intron 30.0%
! TGTTGTGAGTAAAGGGTTTT+AGG + chr3_1:88056396-88056415 Msa0384750:five_prime_UTR 35.0%
GCTTCTGATTCCAATGTAAA+TGG + chr3_1:88056850-88056869 Msa0384750:CDS 35.0%
TTCCAATGTAAATGGTGCAT+TGG + chr3_1:88056858-88056877 Msa0384750:CDS 35.0%
TTCCAATGCACCATTTACAT+TGG - chr3_1:88056863-88056882 None:intergenic 35.0%
! AGTTCTAGTGTTCCTGTTAA+TGG + chr3_1:88056898-88056917 Msa0384750:CDS 35.0%
GTTCTAGTGTTCCTGTTAAT+GGG + chr3_1:88056899-88056918 Msa0384750:CDS 35.0%
TTCTAGTGTTCCTGTTAATG+GGG + chr3_1:88056900-88056919 Msa0384750:CDS 35.0%
CTGTTAATGGGGGTTATAAT+GGG + chr3_1:88056911-88056930 Msa0384750:CDS 35.0%
GAACCATTTCATGGTAAATC+AGG + chr3_1:88056937-88056956 Msa0384750:CDS 35.0%
GAACCTGATTTACCATGAAA+TGG - chr3_1:88056943-88056962 None:intergenic 35.0%
! TCAGGTTCTGTCTCTTTTTA+TGG + chr3_1:88056955-88056974 Msa0384750:CDS 35.0%
! CAGGTTCTGTCTCTTTTTAT+GGG + chr3_1:88056956-88056975 Msa0384750:CDS 35.0%
AAAGATGATATGAATGCGAC+AGG - chr3_1:88057066-88057085 None:intergenic 35.0%
! TGTTGTGAGTAAAGGGTTTT+AGG + chr3_1:88056396-88056415 Msa0384750:five_prime_UTR 35.0%
GCTTCTGATTCCAATGTAAA+TGG + chr3_1:88056850-88056869 Msa0384750:CDS 35.0%
TTCCAATGTAAATGGTGCAT+TGG + chr3_1:88056858-88056877 Msa0384750:CDS 35.0%
TTCCAATGCACCATTTACAT+TGG - chr3_1:88056863-88056882 None:intergenic 35.0%
! AGTTCTAGTGTTCCTGTTAA+TGG + chr3_1:88056898-88056917 Msa0384750:CDS 35.0%
GTTCTAGTGTTCCTGTTAAT+GGG + chr3_1:88056899-88056918 Msa0384750:CDS 35.0%
TTCTAGTGTTCCTGTTAATG+GGG + chr3_1:88056900-88056919 Msa0384750:CDS 35.0%
CTGTTAATGGGGGTTATAAT+GGG + chr3_1:88056911-88056930 Msa0384750:CDS 35.0%
GAACCATTTCATGGTAAATC+AGG + chr3_1:88056937-88056956 Msa0384750:CDS 35.0%
GAACCTGATTTACCATGAAA+TGG - chr3_1:88056943-88056962 None:intergenic 35.0%
! TCAGGTTCTGTCTCTTTTTA+TGG + chr3_1:88056955-88056974 Msa0384750:CDS 35.0%
! CAGGTTCTGTCTCTTTTTAT+GGG + chr3_1:88056956-88056975 Msa0384750:CDS 35.0%
AAAGATGATATGAATGCGAC+AGG - chr3_1:88057066-88057085 None:intergenic 35.0%
! TCAGTTTAGGGTTCAAGAAG+TGG - chr3_1:88056437-88056456 None:intergenic 40.0%
TCCATCGTCACATCAGTTTA+GGG - chr3_1:88056449-88056468 None:intergenic 40.0%
TTCCATCGTCACATCAGTTT+AGG - chr3_1:88056450-88056469 None:intergenic 40.0%
TCTAGTGTTCCTGTTAATGG+GGG + chr3_1:88056901-88056920 Msa0384750:CDS 40.0%
CCATTATAACCCCCATTAAC+AGG - chr3_1:88056913-88056932 None:intergenic 40.0%
CCTGTTAATGGGGGTTATAA+TGG + chr3_1:88056910-88056929 Msa0384750:CDS 40.0%
!! TAATGGGGGTTATAATGGGT+TGG + chr3_1:88056915-88056934 Msa0384750:CDS 40.0%
!! AATGGGTTGGAACCATTTCA+TGG + chr3_1:88056928-88056947 Msa0384750:CDS 40.0%
ACACACCAATCTGTAGAAGA+AGG + chr3_1:88056982-88057001 Msa0384750:CDS 40.0%
CACACCAATCTGTAGAAGAA+GGG + chr3_1:88056983-88057002 Msa0384750:CDS 40.0%
ACTTCCCTTCTTCTACAGAT+TGG - chr3_1:88056990-88057009 None:intergenic 40.0%
ATCTGTAGAAGAAGGGAAGT+TGG + chr3_1:88056990-88057009 Msa0384750:CDS 40.0%
AAGAGGAGAGCTCTTACTTA+TGG + chr3_1:88057031-88057050 Msa0384750:CDS 40.0%
AGAGGAGAGCTCTTACTTAT+GGG + chr3_1:88057032-88057051 Msa0384750:CDS 40.0%
! GAGCTCTTACTTATGGGTTT+TGG + chr3_1:88057038-88057057 Msa0384750:CDS 40.0%
! TCAGTTTAGGGTTCAAGAAG+TGG - chr3_1:88056437-88056456 None:intergenic 40.0%
TCCATCGTCACATCAGTTTA+GGG - chr3_1:88056449-88056468 None:intergenic 40.0%
TTCCATCGTCACATCAGTTT+AGG - chr3_1:88056450-88056469 None:intergenic 40.0%
TCTAGTGTTCCTGTTAATGG+GGG + chr3_1:88056901-88056920 Msa0384750:CDS 40.0%
CCATTATAACCCCCATTAAC+AGG - chr3_1:88056913-88056932 None:intergenic 40.0%
CCTGTTAATGGGGGTTATAA+TGG + chr3_1:88056910-88056929 Msa0384750:CDS 40.0%
!! TAATGGGGGTTATAATGGGT+TGG + chr3_1:88056915-88056934 Msa0384750:CDS 40.0%
!! AATGGGTTGGAACCATTTCA+TGG + chr3_1:88056928-88056947 Msa0384750:CDS 40.0%
ACACACCAATCTGTAGAAGA+AGG + chr3_1:88056982-88057001 Msa0384750:CDS 40.0%
CACACCAATCTGTAGAAGAA+GGG + chr3_1:88056983-88057002 Msa0384750:CDS 40.0%
ACTTCCCTTCTTCTACAGAT+TGG - chr3_1:88056990-88057009 None:intergenic 40.0%
ATCTGTAGAAGAAGGGAAGT+TGG + chr3_1:88056990-88057009 Msa0384750:CDS 40.0%
AAGAGGAGAGCTCTTACTTA+TGG + chr3_1:88057031-88057050 Msa0384750:CDS 40.0%
AGAGGAGAGCTCTTACTTAT+GGG + chr3_1:88057032-88057051 Msa0384750:CDS 40.0%
! GAGCTCTTACTTATGGGTTT+TGG + chr3_1:88057038-88057057 Msa0384750:CDS 40.0%
ACCCTAAACTGATGTGACGA+TGG + chr3_1:88056445-88056464 Msa0384750:exon 45.0%
GAGAAGTGAAGTGGTTGAGA+GGG - chr3_1:88056497-88056516 None:intergenic 45.0%
CGATGTTTGGAGAAGTGAAG+TGG - chr3_1:88056506-88056525 None:intergenic 45.0%
ATCTGCTCCGTTCAAACAAG+AGG + chr3_1:88057014-88057033 Msa0384750:CDS 45.0%
! GAGCTCTCCTCTTGTTTGAA+CGG - chr3_1:88057024-88057043 None:intergenic 45.0%
ATTCTGCCACAGTTCTTCGT+TGG + chr3_1:88057087-88057106 Msa0384750:CDS 45.0%
ACCCTAAACTGATGTGACGA+TGG + chr3_1:88056445-88056464 Msa0384750:exon 45.0%
GAGAAGTGAAGTGGTTGAGA+GGG - chr3_1:88056497-88056516 None:intergenic 45.0%
CGATGTTTGGAGAAGTGAAG+TGG - chr3_1:88056506-88056525 None:intergenic 45.0%
ATCTGCTCCGTTCAAACAAG+AGG + chr3_1:88057014-88057033 Msa0384750:CDS 45.0%
! GAGCTCTCCTCTTGTTTGAA+CGG - chr3_1:88057024-88057043 None:intergenic 45.0%
ATTCTGCCACAGTTCTTCGT+TGG + chr3_1:88057087-88057106 Msa0384750:CDS 45.0%
GGAGAAGTGAAGTGGTTGAG+AGG - chr3_1:88056498-88056517 None:intergenic 50.0%
CTTTCGCAGTCACAAGCAAC+TGG + chr3_1:88056615-88056634 Msa0384750:intron 50.0%
ACAGTGCCAACGAAGAACTG+TGG - chr3_1:88057096-88057115 None:intergenic 50.0%
CTTCGTTGGCACTGTTGTTG+CGG + chr3_1:88057101-88057120 Msa0384750:CDS 50.0%
GGAGAAGTGAAGTGGTTGAG+AGG - chr3_1:88056498-88056517 None:intergenic 50.0%
CTTTCGCAGTCACAAGCAAC+TGG + chr3_1:88056615-88056634 Msa0384750:intron 50.0%
ACAGTGCCAACGAAGAACTG+TGG - chr3_1:88057096-88057115 None:intergenic 50.0%
CTTCGTTGGCACTGTTGTTG+CGG + chr3_1:88057101-88057120 Msa0384750:CDS 50.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 88056382 88057191 88056382 ID=Msa0384750;Name=Msa0384750
chr3_1 mRNA 88056382 88057191 88056382 ID=Msa0384750-mRNA-1;Parent=Msa0384750;Name=Msa0384750-mRNA-1;_AED=0.14;_eAED=0.14;_QI=82|1|0.66|1|1|1|3|0|183
chr3_1 exon 88056382 88056526 88056382 ID=Msa0384750-mRNA-1:exon:23663;Parent=Msa0384750-mRNA-1
chr3_1 exon 88056624 88056696 88056624 ID=Msa0384750-mRNA-1:exon:23664;Parent=Msa0384750-mRNA-1
chr3_1 exon 88056776 88057191 88056776 ID=Msa0384750-mRNA-1:exon:23665;Parent=Msa0384750-mRNA-1
chr3_1 five_prime_UTR 88056382 88056463 88056382 ID=Msa0384750-mRNA-1:five_prime_utr;Parent=Msa0384750-mRNA-1
chr3_1 CDS 88056464 88056526 88056464 ID=Msa0384750-mRNA-1:cds;Parent=Msa0384750-mRNA-1
chr3_1 CDS 88056624 88056696 88056624 ID=Msa0384750-mRNA-1:cds;Parent=Msa0384750-mRNA-1
chr3_1 CDS 88056776 88057191 88056776 ID=Msa0384750-mRNA-1:cds;Parent=Msa0384750-mRNA-1
Gene Sequence

>Msa0384750

ATGGAACTTCAAACTCTTTGTTGCGCTCTTCCCTCTCAACCACTTCACTTCTCCAAACATCGTTCACAAGCAACTGGAACTCAAGCTCTATTCAGATATCAGAAACAGAATTTTGGGTTTGAAAAATCATCAAAATCATTCAAACAGGTGATTAAAGAACCTTCATTTTTGGGATTGAAAAAATTTCAAAGGACTATTACACTTACACATGCTTCTGATTCCAATGTAAATGGTGCATTGGAAGTTGAACTGAAACAAAGTTCTAGTGTTCCTGTTAATGGGGGTTATAATGGGTTGGAACCATTTCATGGTAAATCAGGTTCTGTCTCTTTTTATGGGTTGACACACCAATCTGTAGAAGAAGGGAAGTTGGTATCTGCTCCGTTCAAACAAGAGGAGAGCTCTTACTTATGGGTTTTGGCTCCTGTCGCATTCATATCATCTTTGATTCTGCCACAGTTCTTCGTTGGCACTGTTGTTGCGGCTTTCTTTAATGATTTGATTTTAAAAGGTATTTATGATAGAACTCTATGCTTTCTTTTACTATGTTGA

Protein sequence

>Msa0384750

MELQTLCCALPSQPLHFSKHRSQATGTQALFRYQKQNFGFEKSSKSFKQVIKEPSFLGLKKFQRTITLTHASDSNVNGALEVELKQSSSVPVNGGYNGLEPFHGKSGSVSFYGLTHQSVEEGKLVSAPFKQEESSYLWVLAPVAFISSLILPQFFVGTVVAAFFNDLILKGIYDRTLCFLLLC*