AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0748920


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0748920 MtrunA17_Chr6g0487221 93.023 172 11 1 1 172 39 209 2.73e-102 292
Msa0748920 MtrunA17_Chr8g0389751 50.617 162 57 6 1 147 1 154 4.90e-29 104
Msa0748920 MtrunA17_Chr3g0109691 52.174 92 31 2 65 156 76 154 1.49e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0748920 AT5G56550.1 58.427 89 33 2 62 146 72 160 1.88e-24 94.0
Msa0748920 AT4G26288.1 51.887 106 30 5 47 144 40 132 5.51e-16 71.6

Find 26 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAGAGATAGTTTGTTTA+AGG 0.157965 5_2:+2296070 Msa0748920:three_prime_UTR
GATGGAATTGCATACTTTAT+AGG 0.293344 5_2:-2295164 None:intergenic
CCCTTAAGTTCATCTTTATT+AGG 0.294928 5_2:+2295977 Msa0748920:CDS
CCTAATAAAGATGAACTTAA+GGG 0.332817 5_2:-2295977 None:intergenic
AAACATGAAGTGGGTGGTTA+AGG 0.347693 5_2:+2294977 Msa0748920:CDS
AGTGATTCAACTACATCTAT+TGG 0.349622 5_2:+2295008 Msa0748920:CDS
GCCTAATAAAGATGAACTTA+AGG 0.352869 5_2:-2295978 None:intergenic
AACACAATCTAGGATATAAT+TGG 0.364081 5_2:+2294796 None:intergenic
AATGAATGTGAAGGGAATAA+TGG 0.371118 5_2:+2294938 Msa0748920:exon
ATGAAGTCATGTAGAAGTTA+TGG 0.374322 5_2:+2295884 Msa0748920:CDS
TAGAAGTTATGGAGGAGGTT+TGG 0.410155 5_2:+2295895 Msa0748920:CDS
GTTTGGATAATCATAGAATA+TGG 0.415366 5_2:+2295912 Msa0748920:CDS
TCTTCATCATCACATTCAAA+TGG 0.424478 5_2:+2295104 Msa0748920:CDS
TTCTTGTGTACAACAATTGA+AGG 0.446183 5_2:-2296031 None:intergenic
TCATCTTTATTAGGCAAAAG+AGG 0.482739 5_2:+2295986 Msa0748920:CDS
ATCTTTCACTTCTTTAGCTG+AGG 0.515540 5_2:+2295811 Msa0748920:CDS
CATCTTTATTAGGCAAAAGA+GGG 0.516192 5_2:+2295987 Msa0748920:CDS
ATTTCTTGAAATGAATGTGA+AGG 0.520984 5_2:+2294929 Msa0748920:exon
TCATGTAGAAGTTATGGAGG+AGG 0.533647 5_2:+2295890 Msa0748920:CDS
TTTCTTGAAATGAATGTGAA+GGG 0.535143 5_2:+2294930 Msa0748920:exon
AAGTCATGTAGAAGTTATGG+AGG 0.581965 5_2:+2295887 Msa0748920:CDS
TAGAATATGGTATAGTCCAA+AGG 0.592131 5_2:+2295925 Msa0748920:CDS
TTGTTCATCAGATTTGACTG+AGG 0.665115 5_2:+2295067 Msa0748920:CDS
TGCAAGAAACATGAAGTGGG+TGG 0.681358 5_2:+2294971 Msa0748920:CDS
ATAGTGCAAGAAACATGAAG+TGG 0.703522 5_2:+2294967 Msa0748920:CDS
TAGTGCAAGAAACATGAAGT+GGG 0.728595 5_2:+2294968 Msa0748920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTTAAAATTATTAT+CGG + chr5_2:2295652-2295671 Msa0748920:intron 0.0%
!!! TTATTTTTTAAAATTATTAT+CGG + chr5_2:2295652-2295671 Msa0748920:intron 0.0%
!! AATAAAACTTATAAAAATGT+AGG - chr5_2:2295189-2295208 None:intergenic 10.0%
!! AATAAAACTTATAAAAATGT+AGG - chr5_2:2295189-2295208 None:intergenic 10.0%
!! TAAACTAATAAATACGTAAA+AGG + chr5_2:2295470-2295489 Msa0748920:intron 15.0%
!! TAAACTAATAAATACGTAAA+AGG + chr5_2:2295470-2295489 Msa0748920:intron 15.0%
!! AAACTTATAAAAATGTAGGA+TGG - chr5_2:2295185-2295204 None:intergenic 20.0%
!! CATAATTGAATTTGATTCTT+CGG - chr5_2:2295342-2295361 None:intergenic 20.0%
!!! AATTAGAGATAGTTTGTTTA+AGG + chr5_2:2296070-2296089 Msa0748920:three_prime_UTR 20.0%
!! AAACTTATAAAAATGTAGGA+TGG - chr5_2:2295185-2295204 None:intergenic 20.0%
!! CATAATTGAATTTGATTCTT+CGG - chr5_2:2295342-2295361 None:intergenic 20.0%
!!! AATTAGAGATAGTTTGTTTA+AGG + chr5_2:2296070-2296089 Msa0748920:three_prime_UTR 20.0%
! ATTTCTTGAAATGAATGTGA+AGG + chr5_2:2294929-2294948 Msa0748920:exon 25.0%
! TTTCTTGAAATGAATGTGAA+GGG + chr5_2:2294930-2294949 Msa0748920:exon 25.0%
! AAGGAATCATAGTTATTACA+AGG + chr5_2:2295489-2295508 Msa0748920:intron 25.0%
!! CTGTTCTCTTTTGATAAAAA+TGG - chr5_2:2295565-2295584 None:intergenic 25.0%
! TTTAAGAATATGACACGATA+CGG + chr5_2:2295604-2295623 Msa0748920:intron 25.0%
!!! TGTTTTTAGATGTAGTGAAT+TGG + chr5_2:2295714-2295733 Msa0748920:intron 25.0%
!!! AATTAGTTTATGACTTTTGC+AGG + chr5_2:2295753-2295772 Msa0748920:intron 25.0%
!! TTGTAAGATATGCTTTTCTT+GGG - chr5_2:2295857-2295876 None:intergenic 25.0%
! GTTTGGATAATCATAGAATA+TGG + chr5_2:2295912-2295931 Msa0748920:CDS 25.0%
!!! TTTTTTGATATTGTAGCCTT+TGG - chr5_2:2295944-2295963 None:intergenic 25.0%
! ATATCAAAAAAAGCTTCAAG+AGG + chr5_2:2295953-2295972 Msa0748920:CDS 25.0%
! TATCAAAAAAAGCTTCAAGA+GGG + chr5_2:2295954-2295973 Msa0748920:CDS 25.0%
! CCTAATAAAGATGAACTTAA+GGG - chr5_2:2295980-2295999 None:intergenic 25.0%
! ATTTCTTGAAATGAATGTGA+AGG + chr5_2:2294929-2294948 Msa0748920:exon 25.0%
! TTTCTTGAAATGAATGTGAA+GGG + chr5_2:2294930-2294949 Msa0748920:exon 25.0%
! AAGGAATCATAGTTATTACA+AGG + chr5_2:2295489-2295508 Msa0748920:intron 25.0%
!! CTGTTCTCTTTTGATAAAAA+TGG - chr5_2:2295565-2295584 None:intergenic 25.0%
! TTTAAGAATATGACACGATA+CGG + chr5_2:2295604-2295623 Msa0748920:intron 25.0%
!!! TGTTTTTAGATGTAGTGAAT+TGG + chr5_2:2295714-2295733 Msa0748920:intron 25.0%
!!! AATTAGTTTATGACTTTTGC+AGG + chr5_2:2295753-2295772 Msa0748920:intron 25.0%
!! TTGTAAGATATGCTTTTCTT+GGG - chr5_2:2295857-2295876 None:intergenic 25.0%
! GTTTGGATAATCATAGAATA+TGG + chr5_2:2295912-2295931 Msa0748920:CDS 25.0%
!!! TTTTTTGATATTGTAGCCTT+TGG - chr5_2:2295944-2295963 None:intergenic 25.0%
! ATATCAAAAAAAGCTTCAAG+AGG + chr5_2:2295953-2295972 Msa0748920:CDS 25.0%
! TATCAAAAAAAGCTTCAAGA+GGG + chr5_2:2295954-2295973 Msa0748920:CDS 25.0%
! CCTAATAAAGATGAACTTAA+GGG - chr5_2:2295980-2295999 None:intergenic 25.0%
! TACAACATAACGTGTTTTCA+AGG + chr5_2:2294874-2294893 Msa0748920:five_prime_UTR 30.0%
AATGAATGTGAAGGGAATAA+TGG + chr5_2:2294938-2294957 Msa0748920:exon 30.0%
AGTGATTCAACTACATCTAT+TGG + chr5_2:2295008-2295027 Msa0748920:CDS 30.0%
TCTTCATCATCACATTCAAA+TGG + chr5_2:2295104-2295123 Msa0748920:CDS 30.0%
GATGGAATTGCATACTTTAT+AGG - chr5_2:2295167-2295186 None:intergenic 30.0%
!!! GTCTAGTTTAGACTAGTTTT+TGG + chr5_2:2295261-2295280 Msa0748920:intron 30.0%
! GGTTGTCAATGTTTTATCAA+GGG + chr5_2:2295780-2295799 Msa0748920:CDS 30.0%
! GTTGTAAGATATGCTTTTCT+TGG - chr5_2:2295858-2295877 None:intergenic 30.0%
ATGAAGTCATGTAGAAGTTA+TGG + chr5_2:2295884-2295903 Msa0748920:CDS 30.0%
TAGAATATGGTATAGTCCAA+AGG + chr5_2:2295925-2295944 Msa0748920:CDS 30.0%
CCCTTAAGTTCATCTTTATT+AGG + chr5_2:2295977-2295996 Msa0748920:CDS 30.0%
GCCTAATAAAGATGAACTTA+AGG - chr5_2:2295981-2296000 None:intergenic 30.0%
TCATCTTTATTAGGCAAAAG+AGG + chr5_2:2295986-2296005 Msa0748920:CDS 30.0%
CATCTTTATTAGGCAAAAGA+GGG + chr5_2:2295987-2296006 Msa0748920:CDS 30.0%
TTCTTGTGTACAACAATTGA+AGG - chr5_2:2296034-2296053 None:intergenic 30.0%
! TACAACATAACGTGTTTTCA+AGG + chr5_2:2294874-2294893 Msa0748920:five_prime_UTR 30.0%
AATGAATGTGAAGGGAATAA+TGG + chr5_2:2294938-2294957 Msa0748920:exon 30.0%
AGTGATTCAACTACATCTAT+TGG + chr5_2:2295008-2295027 Msa0748920:CDS 30.0%
TCTTCATCATCACATTCAAA+TGG + chr5_2:2295104-2295123 Msa0748920:CDS 30.0%
GATGGAATTGCATACTTTAT+AGG - chr5_2:2295167-2295186 None:intergenic 30.0%
!!! GTCTAGTTTAGACTAGTTTT+TGG + chr5_2:2295261-2295280 Msa0748920:intron 30.0%
! GGTTGTCAATGTTTTATCAA+GGG + chr5_2:2295780-2295799 Msa0748920:CDS 30.0%
! GTTGTAAGATATGCTTTTCT+TGG - chr5_2:2295858-2295877 None:intergenic 30.0%
ATGAAGTCATGTAGAAGTTA+TGG + chr5_2:2295884-2295903 Msa0748920:CDS 30.0%
TAGAATATGGTATAGTCCAA+AGG + chr5_2:2295925-2295944 Msa0748920:CDS 30.0%
CCCTTAAGTTCATCTTTATT+AGG + chr5_2:2295977-2295996 Msa0748920:CDS 30.0%
GCCTAATAAAGATGAACTTA+AGG - chr5_2:2295981-2296000 None:intergenic 30.0%
TCATCTTTATTAGGCAAAAG+AGG + chr5_2:2295986-2296005 Msa0748920:CDS 30.0%
CATCTTTATTAGGCAAAAGA+GGG + chr5_2:2295987-2296006 Msa0748920:CDS 30.0%
TTCTTGTGTACAACAATTGA+AGG - chr5_2:2296034-2296053 None:intergenic 30.0%
ATAGTGCAAGAAACATGAAG+TGG + chr5_2:2294967-2294986 Msa0748920:CDS 35.0%
TAGTGCAAGAAACATGAAGT+GGG + chr5_2:2294968-2294987 Msa0748920:CDS 35.0%
TTGTTCATCAGATTTGACTG+AGG + chr5_2:2295067-2295086 Msa0748920:CDS 35.0%
TAGTCTAAACTAGACATACG+TGG - chr5_2:2295256-2295275 None:intergenic 35.0%
! TTTATGACTTTTGCAGGAGA+GGG + chr5_2:2295759-2295778 Msa0748920:intron 35.0%
! GGGTTGTCAATGTTTTATCA+AGG + chr5_2:2295779-2295798 Msa0748920:CDS 35.0%
! GTCAATGTTTTATCAAGGGA+AGG + chr5_2:2295784-2295803 Msa0748920:CDS 35.0%
ATCTTTCACTTCTTTAGCTG+AGG + chr5_2:2295811-2295830 Msa0748920:CDS 35.0%
AAGTCATGTAGAAGTTATGG+AGG + chr5_2:2295887-2295906 Msa0748920:CDS 35.0%
ATAGTGCAAGAAACATGAAG+TGG + chr5_2:2294967-2294986 Msa0748920:CDS 35.0%
TAGTGCAAGAAACATGAAGT+GGG + chr5_2:2294968-2294987 Msa0748920:CDS 35.0%
TTGTTCATCAGATTTGACTG+AGG + chr5_2:2295067-2295086 Msa0748920:CDS 35.0%
TAGTCTAAACTAGACATACG+TGG - chr5_2:2295256-2295275 None:intergenic 35.0%
! TTTATGACTTTTGCAGGAGA+GGG + chr5_2:2295759-2295778 Msa0748920:intron 35.0%
! GGGTTGTCAATGTTTTATCA+AGG + chr5_2:2295779-2295798 Msa0748920:CDS 35.0%
! GTCAATGTTTTATCAAGGGA+AGG + chr5_2:2295784-2295803 Msa0748920:CDS 35.0%
ATCTTTCACTTCTTTAGCTG+AGG + chr5_2:2295811-2295830 Msa0748920:CDS 35.0%
AAGTCATGTAGAAGTTATGG+AGG + chr5_2:2295887-2295906 Msa0748920:CDS 35.0%
AAACATGAAGTGGGTGGTTA+AGG + chr5_2:2294977-2294996 Msa0748920:CDS 40.0%
! GTTTATGACTTTTGCAGGAG+AGG + chr5_2:2295758-2295777 Msa0748920:intron 40.0%
TCATGTAGAAGTTATGGAGG+AGG + chr5_2:2295890-2295909 Msa0748920:CDS 40.0%
TAGAAGTTATGGAGGAGGTT+TGG + chr5_2:2295895-2295914 Msa0748920:CDS 40.0%
AAACATGAAGTGGGTGGTTA+AGG + chr5_2:2294977-2294996 Msa0748920:CDS 40.0%
! GTTTATGACTTTTGCAGGAG+AGG + chr5_2:2295758-2295777 Msa0748920:intron 40.0%
TCATGTAGAAGTTATGGAGG+AGG + chr5_2:2295890-2295909 Msa0748920:CDS 40.0%
TAGAAGTTATGGAGGAGGTT+TGG + chr5_2:2295895-2295914 Msa0748920:CDS 40.0%
TGCAAGAAACATGAAGTGGG+TGG + chr5_2:2294971-2294990 Msa0748920:CDS 45.0%
TGCAAGAAACATGAAGTGGG+TGG + chr5_2:2294971-2294990 Msa0748920:CDS 45.0%
!! TATATATATATAAGAATTAT+TGG - chr5_2:2294832-2294851 None:intergenic 5.0%
!! TATATATATATAAGAATTAT+TGG - chr5_2:2294832-2294851 None:intergenic 5.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 2294804 2296142 2294804 ID=Msa0748920;Name=Msa0748920
chr5_2 mRNA 2294804 2296142 2294804 ID=Msa0748920-mRNA-1;Parent=Msa0748920;Name=Msa0748920-mRNA-1;_AED=0.01;_eAED=0.01;_QI=135|1|1|1|1|1|2|82|172
chr5_2 exon 2294804 2295171 2294804 ID=Msa0748920-mRNA-1:exon:657;Parent=Msa0748920-mRNA-1
chr5_2 exon 2295775 2296142 2295775 ID=Msa0748920-mRNA-1:exon:658;Parent=Msa0748920-mRNA-1
chr5_2 five_prime_UTR 2294804 2294938 2294804 ID=Msa0748920-mRNA-1:five_prime_utr;Parent=Msa0748920-mRNA-1
chr5_2 CDS 2294939 2295171 2294939 ID=Msa0748920-mRNA-1:cds;Parent=Msa0748920-mRNA-1
chr5_2 CDS 2295775 2296060 2295775 ID=Msa0748920-mRNA-1:cds;Parent=Msa0748920-mRNA-1
chr5_2 three_prime_UTR 2296061 2296142 2296061 ID=Msa0748920-mRNA-1:three_prime_utr;Parent=Msa0748920-mRNA-1
Gene Sequence

>Msa0748920

ATGAATGTGAAGGGAATAATGGATGATGATAGTGCAAGAAACATGAAGTGGGTGGTTAAGGAAAGTAATAGTGATTCAACTACATCTATTGGAACATTCTCAGAAGATTCAAATAATTCTATGTGTTCTTGTTCATCAGATTTGACTGAGGATGCTGATTCTTCTTCTTCATCATCACATTCAAATGGATCATTATGTGATTTCTCAGAGCTCATGAACAATCTTCCTATAAAGAGAGGGTTGTCAATGTTTTATCAAGGGAAGGCTCAATCTTTCACTTCTTTAGCTGAGGTACAAAACATAGAAGATCTTCCCAAGAAAAGCATATCTTACAACAAAAGAATGAAGTCATGTAGAAGTTATGGAGGAGGTTTGGATAATCATAGAATATGGTATAGTCCAAAGGCTACAATATCAAAAAAAGCTTCAAGAGGGCCCTTAAGTTCATCTTTATTAGGCAAAAGAGGGAGTTTATTAGAACGTTCGAGACCTTCAATTGTTGTACACAAGAACTTCTGA

Protein sequence

>Msa0748920

MNVKGIMDDDSARNMKWVVKESNSDSTTSIGTFSEDSNNSMCSCSSDLTEDADSSSSSSHSNGSLCDFSELMNNLPIKRGLSMFYQGKAQSFTSLAEVQNIEDLPKKSISYNKRMKSCRSYGGGLDNHRIWYSPKATISKKASRGPLSSSLLGKRGSLLERSRPSIVVHKNF*