AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0492930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTTGAATGTGTTTCTCTT+TGG 0.194399 3_4:+17120481 Msa0492930:three_prime_UTR
GTTTGAATGTGTTTCTCTTT+GGG 0.223170 3_4:+17120482 Msa0492930:three_prime_UTR
GAATATGCCATCTGATTTCA+TGG 0.293561 3_4:+17120529 Msa0492930:three_prime_UTR
GGTCACTGTGAAGGACTATT+TGG 0.325156 3_4:+17120044 Msa0492930:CDS
GGCATATTCTTAAGCCAAAA+TGG 0.333698 3_4:-17120515 None:intergenic
TTTCAGAAGCTTCACATAAA+GGG 0.355501 3_4:+17120115 Msa0492930:three_prime_UTR
TGATGGAACTGAAAGGAAAA+AGG 0.356774 3_4:+17120020 Msa0492930:CDS
ATTTCAGAAGCTTCACATAA+AGG 0.357257 3_4:+17120114 Msa0492930:three_prime_UTR
CCAGAAAAGCATTTGGCATA+TGG 0.435201 3_4:+17117343 Msa0492930:CDS
TCAACGGATATTAGCAAAGA+AGG 0.452435 3_4:-17120588 None:intergenic
ATGCCATCTGATTTCATGGT+TGG 0.454887 3_4:+17120533 Msa0492930:three_prime_UTR
GGAAGAAAATCACTTGACTT+TGG 0.456885 3_4:+17120309 Msa0492930:three_prime_UTR
TCTTGCATCAAGTGGTGCTT+AGG 0.462711 3_4:+17120338 Msa0492930:three_prime_UTR
CATGAATGGTATTGCTGCTA+CGG 0.469678 3_4:-17120658 None:intergenic
ATTTCTCGAGCCTTTCCAAC+CGG 0.474641 3_4:+17117391 Msa0492930:CDS
AGTCCTGCTATCTGTTGTAT+TGG 0.477346 3_4:+17120427 Msa0492930:three_prime_UTR
GTTGTCACAGAAGTTGCATC+TGG 0.478649 3_4:+17120560 Msa0492930:three_prime_UTR
TGTGTGAATAGTCCAGAAAA+AGG 0.478813 3_4:+17120218 Msa0492930:three_prime_UTR
TTTGAATGTGTTTCTCTTTG+GGG 0.478837 3_4:+17120483 Msa0492930:three_prime_UTR
TACAACAGATAGCAGGACTT+TGG 0.483767 3_4:-17120423 None:intergenic
AATGCTGCCAGAAAAGCATT+TGG 0.489977 3_4:+17117336 None:intergenic
GGTCAACTGATCATACTTGT+TGG 0.497649 3_4:+17119979 Msa0492930:CDS
TTGAAACTTGAAATCATGAA+TGG 0.501154 3_4:-17120672 None:intergenic
ACTATTTGGCTGAGTAACTA+AGG 0.517520 3_4:+17120058 Msa0492930:exon
GCTAGCACTGGTAATAATGA+TGG 0.526475 3_4:+17120003 Msa0492930:CDS
ACTTTGGTTCAACTATTCAC+AGG 0.529114 3_4:-17120407 None:intergenic
TTGAATGTGTTTCTCTTTGG+GGG 0.535537 3_4:+17120484 Msa0492930:three_prime_UTR
AGTGCCTTGTCTTCTTCACT+AGG 0.547320 3_4:-17120081 None:intergenic
ATACTTGTTGGAGCTAGCAC+TGG 0.552542 3_4:+17119991 Msa0492930:CDS
ATATCAGATACCGGTTGGAA+AGG 0.567624 3_4:-17117401 None:intergenic
TCACGACATATATAAAACAT+TGG 0.571352 3_4:+17120254 Msa0492930:three_prime_UTR
AGCACCACTTGATGCAAGAT+CGG 0.581275 3_4:-17120334 None:intergenic
TCAGAGACCTCCATGCCGGT+TGG 0.586854 3_4:+17120288 Msa0492930:three_prime_UTR
CGAGAAATAAATAAAAGCAA+TGG 0.592989 3_4:-17117376 None:intergenic
GGTACCGATCTTGCATCAAG+TGG 0.593584 3_4:+17120330 Msa0492930:three_prime_UTR
CAGTTGTTCTCAGAAATTCA+AGG 0.598435 3_4:+17120364 Msa0492930:three_prime_UTR
AGGCCAATACAACAGATAGC+AGG 0.606423 3_4:-17120430 None:intergenic
CTGATTAAAATCCTCTCTGA+GGG 0.606910 3_4:+17119958 Msa0492930:CDS
AGTCCAACCATGAAATCAGA+TGG 0.609149 3_4:-17120536 None:intergenic
GTAATAATGATGGAACTGAA+AGG 0.611801 3_4:+17120013 Msa0492930:CDS
TCATCATCAACAAACTTCAA+CGG 0.628201 3_4:-17120604 None:intergenic
TCTGATTAAAATCCTCTCTG+AGG 0.628725 3_4:+17119957 Msa0492930:CDS
ATAATCAGAGACCTCCATGC+CGG 0.633204 3_4:+17120284 Msa0492930:three_prime_UTR
GAAATTCAAGGTGATAGCAG+TGG 0.640348 3_4:+17120376 Msa0492930:three_prime_UTR
TTCAAGGTGATAGCAGTGGT+AGG 0.668612 3_4:+17120380 Msa0492930:three_prime_UTR
AAGGCCTAGTGAAGAAGACA+AGG 0.676686 3_4:+17120077 Msa0492930:three_prime_UTR
TGATCAGTTGACCCTCAGAG+AGG 0.717476 3_4:-17119969 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATCTTGTAAATTATAAT+TGG - chr3_4:17118477-17118496 None:intergenic 10.0%
!!! TTGTTCTATATGTATTTTTA+TGG + chr3_4:17118078-17118097 Msa0492930:intron 15.0%
!!! TTGATTTTAAGAGAAAAAAA+TGG + chr3_4:17118519-17118538 Msa0492930:intron 15.0%
!! AGATGAAAATTAATTAACAA+TGG + chr3_4:17118920-17118939 Msa0492930:intron 15.0%
!!! ATAATATTTAATGTTGATTG+AGG - chr3_4:17119308-17119327 None:intergenic 15.0%
!!! ATTAATCATATTTGATTTGT+TGG + chr3_4:17119915-17119934 Msa0492930:intron 15.0%
!! TCTGATAATAAAGTATACAA+CGG + chr3_4:17117719-17117738 Msa0492930:intron 20.0%
!!! TAGCTAAAAAGTCTTTTATT+GGG - chr3_4:17117822-17117841 None:intergenic 20.0%
!! CATATAGAACAAGAATATAA+GGG - chr3_4:17118070-17118089 None:intergenic 20.0%
!! ACATATAGAACAAGAATATA+AGG - chr3_4:17118071-17118090 None:intergenic 20.0%
!! ATGAAAACAAATAGATAATC+TGG - chr3_4:17118226-17118245 None:intergenic 20.0%
!!! TTTGTTTTCATTGTTTACTA+AGG + chr3_4:17118235-17118254 Msa0492930:intron 20.0%
!!! TTTACTTTTCTAATCCATAT+AGG - chr3_4:17118757-17118776 None:intergenic 20.0%
!!! TATATGGATTAGAAAAGTAA+AGG + chr3_4:17118756-17118775 Msa0492930:intron 20.0%
!! TGTACAATATTTAATTGAAC+AGG + chr3_4:17118835-17118854 Msa0492930:intron 20.0%
!!! AAGCTTAATCTGTTTATTTT+TGG + chr3_4:17118958-17118977 Msa0492930:intron 20.0%
!! GACAAAAAAATTTCTCAAAA+GGG - chr3_4:17119202-17119221 None:intergenic 20.0%
!! AGACAAAAAAATTTCTCAAA+AGG - chr3_4:17119203-17119222 None:intergenic 20.0%
!! AAAGAATTAATTTATGCACT+TGG - chr3_4:17119252-17119271 None:intergenic 20.0%
!!! ATATGCGTTATTTTTTCAAA+AGG + chr3_4:17119422-17119441 Msa0492930:intron 20.0%
!!! TATGCGTTATTTTTTCAAAA+GGG + chr3_4:17119423-17119442 Msa0492930:intron 20.0%
! CGAGAAATAAATAAAAGCAA+TGG - chr3_4:17117379-17117398 None:intergenic 25.0%
!! GTAGCTAAAAAGTCTTTTAT+TGG - chr3_4:17117823-17117842 None:intergenic 25.0%
!! CTTTTAACGTTAGACAAATA+GGG + chr3_4:17117843-17117862 Msa0492930:intron 25.0%
!!! TAAAAGTTACGTTGTTTTCA+CGG + chr3_4:17117918-17117937 Msa0492930:intron 25.0%
! TTCCATACAAATTTGAAAAC+AGG + chr3_4:17117996-17118015 Msa0492930:intron 25.0%
!! TTCCTGTTTTCAAATTTGTA+TGG - chr3_4:17118001-17118020 None:intergenic 25.0%
! TCATACCATTGTGTATTATA+GGG - chr3_4:17118174-17118193 None:intergenic 25.0%
! ATAGATAATCTGGTCTATAA+AGG - chr3_4:17118216-17118235 None:intergenic 25.0%
!!! TTTTTAAAACTCCCAAACAA+GGG - chr3_4:17118325-17118344 None:intergenic 25.0%
!!! TTTTTTAAAACTCCCAAACA+AGG - chr3_4:17118326-17118345 None:intergenic 25.0%
!!! TTGGGAGTTTTAAAAAAACA+AGG + chr3_4:17118329-17118348 Msa0492930:intron 25.0%
!!! TGGGAGTTTTAAAAAAACAA+GGG + chr3_4:17118330-17118349 Msa0492930:intron 25.0%
!!! GTTTTAAAAAAACAAGGGAA+AGG + chr3_4:17118335-17118354 Msa0492930:intron 25.0%
!!! TGGAATTTTGAGTTATTATG+AGG - chr3_4:17118457-17118476 None:intergenic 25.0%
!! TCAAAAGGGTCTTATAATTA+GGG - chr3_4:17119188-17119207 None:intergenic 25.0%
! AAACTAACTATCTCTCTTAA+AGG + chr3_4:17119339-17119358 Msa0492930:intron 25.0%
! CGCATATTAAGAAAATTGAT+AGG - chr3_4:17119409-17119428 None:intergenic 25.0%
! GTAATACACAATATTTGCAA+AGG + chr3_4:17119469-17119488 Msa0492930:intron 25.0%
!!! CCATTACTTTTAAGTTGTTT+TGG - chr3_4:17119555-17119574 None:intergenic 25.0%
! CCAAAACAACTTAAAAGTAA+TGG + chr3_4:17119552-17119571 Msa0492930:intron 25.0%
! CATTTACACAACTATAAACA+AGG - chr3_4:17119660-17119679 None:intergenic 25.0%
! CATATATGTTTAAACTAGCT+AGG - chr3_4:17119808-17119827 None:intergenic 25.0%
! TCACGACATATATAAAACAT+TGG + chr3_4:17120254-17120273 Msa0492930:three_prime_UTR 25.0%
!!! TGTATGCAAATCCTTTTTTA+AGG - chr3_4:17120453-17120472 None:intergenic 25.0%
CATATTATAGCATGATCTCA+TGG + chr3_4:17117587-17117606 Msa0492930:intron 30.0%
CCTATTTGTCTAACGTTAAA+AGG - chr3_4:17117845-17117864 None:intergenic 30.0%
! CCTTTTAACGTTAGACAAAT+AGG + chr3_4:17117842-17117861 Msa0492930:intron 30.0%
AATTTGAAAACAGGAAACTG+TGG + chr3_4:17118005-17118024 Msa0492930:intron 30.0%
! ATTTTTATGGCATTTCTACG+TGG + chr3_4:17118091-17118110 Msa0492930:intron 30.0%
CATACCATTGTGTATTATAG+GGG - chr3_4:17118173-17118192 None:intergenic 30.0%
CTCATACCATTGTGTATTAT+AGG - chr3_4:17118175-17118194 None:intergenic 30.0%
CTATAATACACAATGGTATG+AGG + chr3_4:17118173-17118192 Msa0492930:intron 30.0%
! AAAAAACAAGGGAAAGGTTT+TGG + chr3_4:17118341-17118360 Msa0492930:intron 30.0%
! AAAAACAAGGGAAAGGTTTT+GGG + chr3_4:17118342-17118361 Msa0492930:intron 30.0%
!! ATTTGGGGGAACTTTAAAAT+TGG - chr3_4:17118391-17118410 None:intergenic 30.0%
!!! ATTTTAAAGTTCCCCCAAAT+TGG + chr3_4:17118391-17118410 Msa0492930:intron 30.0%
!!! TTTTAAAGTTCCCCCAAATT+GGG + chr3_4:17118392-17118411 Msa0492930:intron 30.0%
TTTCTTTAACTCCCAAACAA+GGG + chr3_4:17118591-17118610 Msa0492930:intron 30.0%
TTCCTTTGTGATTCAGATTA+TGG + chr3_4:17118716-17118735 Msa0492930:intron 30.0%
AACCATAATCTGAATCACAA+AGG - chr3_4:17118721-17118740 None:intergenic 30.0%
! TCTAGTCACTATTTGACTTA+TGG - chr3_4:17118870-17118889 None:intergenic 30.0%
TAAATAAATCATGTCAGAGC+AGG - chr3_4:17118984-17119003 None:intergenic 30.0%
AACTGCAAAATCAATCAGTT+TGG - chr3_4:17119140-17119159 None:intergenic 30.0%
! CTCAAAAGGGTCTTATAATT+AGG - chr3_4:17119189-17119208 None:intergenic 30.0%
! CTCTTTTTATAAGACCCTTT+TGG + chr3_4:17119221-17119240 Msa0492930:intron 30.0%
! AAAAGTAATGGAAGTTGTCT+TGG + chr3_4:17119564-17119583 Msa0492930:intron 30.0%
TAGCATAACTACCATACAAA+GGG - chr3_4:17119628-17119647 None:intergenic 30.0%
ATAGCATAACTACCATACAA+AGG - chr3_4:17119629-17119648 None:intergenic 30.0%
! GTAATAATGATGGAACTGAA+AGG + chr3_4:17120013-17120032 Msa0492930:CDS 30.0%
ATTTCAGAAGCTTCACATAA+AGG + chr3_4:17120114-17120133 Msa0492930:three_prime_UTR 30.0%
TTTCAGAAGCTTCACATAAA+GGG + chr3_4:17120115-17120134 Msa0492930:three_prime_UTR 30.0%
!!! ATTCCATCCTCTTTTTTCAA+TGG - chr3_4:17120150-17120169 None:intergenic 30.0%
!! TGTTGTATTGGCCTTAAAAA+AGG + chr3_4:17120439-17120458 Msa0492930:three_prime_UTR 30.0%
AGTTTGAATGTGTTTCTCTT+TGG + chr3_4:17120481-17120500 Msa0492930:three_prime_UTR 30.0%
GTTTGAATGTGTTTCTCTTT+GGG + chr3_4:17120482-17120501 Msa0492930:three_prime_UTR 30.0%
TTTGAATGTGTTTCTCTTTG+GGG + chr3_4:17120483-17120502 Msa0492930:three_prime_UTR 30.0%
TCATCATCAACAAACTTCAA+CGG - chr3_4:17120607-17120626 None:intergenic 30.0%
TAAACATATCAGATACCGGT+TGG - chr3_4:17117409-17117428 None:intergenic 35.0%
GGGTTAAACATATCAGATAC+CGG - chr3_4:17117413-17117432 None:intergenic 35.0%
TATCTGATATGTTTAACCCG+TGG + chr3_4:17117414-17117433 Msa0492930:intron 35.0%
AAGAATTAGACACTAGTTGC+TGG + chr3_4:17117460-17117479 Msa0492930:intron 35.0%
! TTAGACAAATAGGGCTTGTT+TGG + chr3_4:17117852-17117871 Msa0492930:intron 35.0%
! AGGGTCTGTTTGATTTCAAA+AGG - chr3_4:17118051-17118070 None:intergenic 35.0%
! GCATTTCTACGTGGATTATT+TGG + chr3_4:17118100-17118119 Msa0492930:intron 35.0%
! TCTACGTGGATTATTTGGTA+TGG + chr3_4:17118105-17118124 Msa0492930:intron 35.0%
!!! TTCTTTAGCTTTTCCTGCAT+CGG + chr3_4:17118133-17118152 Msa0492930:intron 35.0%
!! CCTGCATCGGTATTTATTTT+CGG + chr3_4:17118146-17118165 Msa0492930:intron 35.0%
!! CTGCATCGGTATTTATTTTC+GGG + chr3_4:17118147-17118166 Msa0492930:intron 35.0%
!! GTTTACTAAGGCTTTGTTTG+CGG + chr3_4:17118247-17118266 Msa0492930:intron 35.0%
!! TTTACTAAGGCTTTGTTTGC+GGG + chr3_4:17118248-17118267 Msa0492930:intron 35.0%
! AAAACAAGGGAAAGGTTTTG+GGG + chr3_4:17118343-17118362 Msa0492930:intron 35.0%
! TTTAAAGTTCCCCCAAATTG+GGG + chr3_4:17118393-17118412 Msa0492930:intron 35.0%
AAACCAACAAGGAAATGAGA+TGG - chr3_4:17118574-17118593 None:intergenic 35.0%
GTTTCTTTAACTCCCAAACA+AGG + chr3_4:17118590-17118609 Msa0492930:intron 35.0%
TTCTTTAACTCCCAAACAAG+GGG + chr3_4:17118592-17118611 Msa0492930:intron 35.0%
!!! CTTTGTTTGTGAGTTTTGAG+GGG - chr3_4:17118686-17118705 None:intergenic 35.0%
!!! GCTTTGTTTGTGAGTTTTGA+GGG - chr3_4:17118687-17118706 None:intergenic 35.0%
AAACTCACAAACAAAGCCTA+AGG + chr3_4:17118690-17118709 Msa0492930:intron 35.0%
AATCACAAAGGAAACTCCTT+AGG - chr3_4:17118709-17118728 None:intergenic 35.0%
! TTTCAAGTAGCTAGCCTATA+TGG + chr3_4:17118740-17118759 Msa0492930:intron 35.0%
! TATTTGACTTATGGCGTGTT+TGG - chr3_4:17118861-17118880 None:intergenic 35.0%
! GACTAGAACACTTCTTTTGT+TGG + chr3_4:17118883-17118902 Msa0492930:intron 35.0%
! AAGGGTCTTATAATTAGGGA+CGG - chr3_4:17119184-17119203 None:intergenic 35.0%
TGCACTTGGAAATACCAAAA+GGG - chr3_4:17119238-17119257 None:intergenic 35.0%
ATGCACTTGGAAATACCAAA+AGG - chr3_4:17119239-17119258 None:intergenic 35.0%
TTCAAAAGGGCCCTATAATT+AGG + chr3_4:17119436-17119455 Msa0492930:intron 35.0%
TCAAAAGGGCCCTATAATTA+GGG + chr3_4:17119437-17119456 Msa0492930:intron 35.0%
AGCATAACTACCATACAAAG+GGG - chr3_4:17119627-17119646 None:intergenic 35.0%
! CTTTCTCTGCTATTTACATG+AGG - chr3_4:17119872-17119891 None:intergenic 35.0%
!! GATTTTAATCAGAGAGGATG+TGG - chr3_4:17119950-17119969 None:intergenic 35.0%
!! AGAGAGGATTTTAATCAGAG+AGG - chr3_4:17119956-17119975 None:intergenic 35.0%
TCTGATTAAAATCCTCTCTG+AGG + chr3_4:17119957-17119976 Msa0492930:CDS 35.0%
CTGATTAAAATCCTCTCTGA+GGG + chr3_4:17119958-17119977 Msa0492930:CDS 35.0%
TGATGGAACTGAAAGGAAAA+AGG + chr3_4:17120020-17120039 Msa0492930:CDS 35.0%
ACTATTTGGCTGAGTAACTA+AGG + chr3_4:17120058-17120077 Msa0492930:exon 35.0%
!! AGAAGACAAGGCACTTTTTA+AGG + chr3_4:17120089-17120108 Msa0492930:three_prime_UTR 35.0%
AGAAGAGCCATTGAAAAAAG+AGG + chr3_4:17120140-17120159 Msa0492930:three_prime_UTR 35.0%
TGTGTGAATAGTCCAGAAAA+AGG + chr3_4:17120218-17120237 Msa0492930:three_prime_UTR 35.0%
! TCAAGTGATTTTCTTCCAAC+CGG - chr3_4:17120306-17120325 None:intergenic 35.0%
GGAAGAAAATCACTTGACTT+TGG + chr3_4:17120309-17120328 Msa0492930:three_prime_UTR 35.0%
CAGTTGTTCTCAGAAATTCA+AGG + chr3_4:17120364-17120383 Msa0492930:three_prime_UTR 35.0%
! ACTTTGGTTCAACTATTCAC+AGG - chr3_4:17120410-17120429 None:intergenic 35.0%
TTGAATGTGTTTCTCTTTGG+GGG + chr3_4:17120484-17120503 Msa0492930:three_prime_UTR 35.0%
GGCATATTCTTAAGCCAAAA+TGG - chr3_4:17120518-17120537 None:intergenic 35.0%
GAATATGCCATCTGATTTCA+TGG + chr3_4:17120529-17120548 Msa0492930:three_prime_UTR 35.0%
TCAACGGATATTAGCAAAGA+AGG - chr3_4:17120591-17120610 None:intergenic 35.0%
! CCATATGCCAAATGCTTTTC+TGG - chr3_4:17117346-17117365 None:intergenic 40.0%
!! CCAGAAAAGCATTTGGCATA+TGG + chr3_4:17117343-17117362 Msa0492930:CDS 40.0%
! ATATCAGATACCGGTTGGAA+AGG - chr3_4:17117404-17117423 None:intergenic 40.0%
ATGGTATAGAGTATGCAACG+AGG - chr3_4:17117487-17117506 None:intergenic 40.0%
CGTTGCATACTCTATACCAT+TGG + chr3_4:17117487-17117506 Msa0492930:intron 40.0%
ACCATTGGATAGTTGCACTA+TGG + chr3_4:17117502-17117521 Msa0492930:intron 40.0%
TCCATAGTGCAACTATCCAA+TGG - chr3_4:17117506-17117525 None:intergenic 40.0%
ATTGGATAGTTGCACTATGG+AGG + chr3_4:17117505-17117524 Msa0492930:intron 40.0%
GCACTATGGAGGTTACTTAT+CGG + chr3_4:17117516-17117535 Msa0492930:intron 40.0%
TCTAATGAGAGGATGGAGAA+AGG - chr3_4:17117790-17117809 None:intergenic 40.0%
CCTAAGATCTAATGAGAGGA+TGG - chr3_4:17117797-17117816 None:intergenic 40.0%
CCATCCTCTCATTAGATCTT+AGG + chr3_4:17117794-17117813 Msa0492930:intron 40.0%
CCGAAAATAAATACCGATGC+AGG - chr3_4:17118149-17118168 None:intergenic 40.0%
GAAAGGAAAGGCTTATGGAA+AGG + chr3_4:17118295-17118314 Msa0492930:intron 40.0%
TGGAAAGGTAAACCCTTGTT+TGG + chr3_4:17118310-17118329 Msa0492930:intron 40.0%
! GGAAAGGTAAACCCTTGTTT+GGG + chr3_4:17118311-17118330 Msa0492930:intron 40.0%
!! AAACAAGGGAAAGGTTTTGG+GGG + chr3_4:17118344-17118363 Msa0492930:intron 40.0%
TTAAAGTTCCCCCAAATTGG+GGG + chr3_4:17118394-17118413 Msa0492930:intron 40.0%
CAACAAGGAAATGAGATGGA+GGG - chr3_4:17118570-17118589 None:intergenic 40.0%
GGGAGTTAAAGAAACCAACA+AGG - chr3_4:17118585-17118604 None:intergenic 40.0%
!!! GGCTTTGTTTGTGAGTTTTG+AGG - chr3_4:17118688-17118707 None:intergenic 40.0%
ACCATTGAATTCAACGTCTC+AGG + chr3_4:17118788-17118807 Msa0492930:intron 40.0%
ACCTGAGACGTTGAATTCAA+TGG - chr3_4:17118792-17118811 None:intergenic 40.0%
TACCTCCGTCCCTAATTATA+GGG - chr3_4:17119176-17119195 None:intergenic 40.0%
ATATTTGACCCCCCTTTGTA+TGG + chr3_4:17119614-17119633 Msa0492930:intron 40.0%
CATAACTACCATACAAAGGG+GGG - chr3_4:17119625-17119644 None:intergenic 40.0%
GCATAACTACCATACAAAGG+GGG - chr3_4:17119626-17119645 None:intergenic 40.0%
GTCTCGACTTAGTGTATAAG+AGG + chr3_4:17119765-17119784 Msa0492930:intron 40.0%
GAGAAAGAAATGCGAGTTCA+TGG + chr3_4:17119885-17119904 Msa0492930:intron 40.0%
GGTCAACTGATCATACTTGT+TGG + chr3_4:17119979-17119998 Msa0492930:CDS 40.0%
!! GCTAGCACTGGTAATAATGA+TGG + chr3_4:17120003-17120022 Msa0492930:CDS 40.0%
TGGAACTGAAAGGAAAAAGG+AGG + chr3_4:17120023-17120042 Msa0492930:CDS 40.0%
GAGCCATTGAAAAAAGAGGA+TGG + chr3_4:17120144-17120163 Msa0492930:three_prime_UTR 40.0%
GAATAGTCCAGAAAAAGGTG+TGG + chr3_4:17120223-17120242 Msa0492930:three_prime_UTR 40.0%
! TGACTATCCACACCTTTTTC+TGG - chr3_4:17120233-17120252 None:intergenic 40.0%
GAAATTCAAGGTGATAGCAG+TGG + chr3_4:17120376-17120395 Msa0492930:three_prime_UTR 40.0%
TACAACAGATAGCAGGACTT+TGG - chr3_4:17120426-17120445 None:intergenic 40.0%
AGTCCTGCTATCTGTTGTAT+TGG + chr3_4:17120427-17120446 Msa0492930:three_prime_UTR 40.0%
ATGCCATCTGATTTCATGGT+TGG + chr3_4:17120533-17120552 Msa0492930:three_prime_UTR 40.0%
AGTCCAACCATGAAATCAGA+TGG - chr3_4:17120539-17120558 None:intergenic 40.0%
CATGAATGGTATTGCTGCTA+CGG - chr3_4:17120661-17120680 None:intergenic 40.0%
ATTTCTCGAGCCTTTCCAAC+CGG + chr3_4:17117391-17117410 Msa0492930:CDS 45.0%
TGGAGGTTACTTATCGGATC+TGG + chr3_4:17117522-17117541 Msa0492930:intron 45.0%
AATGAGAGGATGGAGAAAGG+TGG - chr3_4:17117787-17117806 None:intergenic 45.0%
GGCTCCTAAGATCTAATGAG+AGG - chr3_4:17117801-17117820 None:intergenic 45.0%
!! TAAGGCTTTGTTTGCGGGTT+GGG + chr3_4:17118253-17118272 Msa0492930:intron 45.0%
!!! TTTGTTTGCGGGTTGGGTTT+TGG + chr3_4:17118259-17118278 Msa0492930:intron 45.0%
!!! AACAAGGGAAAGGTTTTGGG+GGG + chr3_4:17118345-17118364 Msa0492930:intron 45.0%
!!! AAGGTTTTGGGGGGTTTTGA+AGG + chr3_4:17118354-17118373 Msa0492930:intron 45.0%
!!! AGGTTTTGGGGGGTTTTGAA+GGG + chr3_4:17118355-17118374 Msa0492930:intron 45.0%
TAAAGTTCCCCCAAATTGGG+GGG + chr3_4:17118395-17118414 Msa0492930:intron 45.0%
AATGAGATGGAGGGCTTATG+TGG - chr3_4:17118561-17118580 None:intergenic 45.0%
CCAACAAGGAAATGAGATGG+AGG - chr3_4:17118571-17118590 None:intergenic 45.0%
CCTCCATCTCATTTCCTTGT+TGG + chr3_4:17118568-17118587 Msa0492930:intron 45.0%
TTAACTCCCAAACAAGGGGA+AGG + chr3_4:17118596-17118615 Msa0492930:intron 45.0%
! GGAAAACCTTCCCCTTGTTT+GGG - chr3_4:17118605-17118624 None:intergenic 45.0%
AGGAAAGGAAAGGCTAAGGA+GGG - chr3_4:17118662-17118681 None:intergenic 45.0%
AAGGAAAGGAAAGGCTAAGG+AGG - chr3_4:17118663-17118682 None:intergenic 45.0%
TTTGAGGGGAAGGAAAGGAA+AGG - chr3_4:17118672-17118691 None:intergenic 45.0%
!!! TGAGTTTTGAGGGGAAGGAA+AGG - chr3_4:17118677-17118696 None:intergenic 45.0%
!!! GTTTGTGAGTTTTGAGGGGA+AGG - chr3_4:17118682-17118701 None:intergenic 45.0%
! GGTCTTATAATTAGGGACGG+AGG - chr3_4:17119181-17119200 None:intergenic 45.0%
AAGGGCCCTATAATTAGGGA+CGG + chr3_4:17119441-17119460 Msa0492930:intron 45.0%
CTACCTCCGTCCCTAATTAT+AGG - chr3_4:17119175-17119194 None:intergenic 45.0%
TAAGTCGAGACTGCTATCAG+AGG - chr3_4:17119756-17119775 None:intergenic 45.0%
! ATACTTGTTGGAGCTAGCAC+TGG + chr3_4:17119991-17120010 Msa0492930:CDS 45.0%
GAAAAAGGAGGTCACTGTGA+AGG + chr3_4:17120035-17120054 Msa0492930:CDS 45.0%
GGTCACTGTGAAGGACTATT+TGG + chr3_4:17120044-17120063 Msa0492930:CDS 45.0%
AAGGCCTAGTGAAGAAGACA+AGG + chr3_4:17120077-17120096 Msa0492930:three_prime_UTR 45.0%
AGTGCCTTGTCTTCTTCACT+AGG - chr3_4:17120084-17120103 None:intergenic 45.0%
ATAATCAGAGACCTCCATGC+CGG + chr3_4:17120284-17120303 Msa0492930:three_prime_UTR 45.0%
! TGATTTTCTTCCAACCGGCA+TGG - chr3_4:17120301-17120320 None:intergenic 45.0%
AGCACCACTTGATGCAAGAT+CGG - chr3_4:17120337-17120356 None:intergenic 45.0%
TCTTGCATCAAGTGGTGCTT+AGG + chr3_4:17120338-17120357 Msa0492930:three_prime_UTR 45.0%
TTCAAGGTGATAGCAGTGGT+AGG + chr3_4:17120380-17120399 Msa0492930:three_prime_UTR 45.0%
AGGCCAATACAACAGATAGC+AGG - chr3_4:17120433-17120452 None:intergenic 45.0%
! GTTTCTCTTTGGGGGTAAAG+TGG + chr3_4:17120492-17120511 Msa0492930:three_prime_UTR 45.0%
GTTGTCACAGAAGTTGCATC+TGG + chr3_4:17120560-17120579 Msa0492930:three_prime_UTR 45.0%
!! AAAAGATATAAAATAAATAA+GGG - chr3_4:17119284-17119303 None:intergenic 5.0%
!! AAAAAGATATAAAATAAATA+AGG - chr3_4:17119285-17119304 None:intergenic 5.0%
AAGTCAAGTGTTCGCAGCCA+CGG - chr3_4:17117434-17117453 None:intergenic 50.0%
ATCTCATGGTTGCTTCGTCG+AGG + chr3_4:17117601-17117620 Msa0492930:intron 50.0%
! GGTTGCTTCGTCGAGGTATT+TGG + chr3_4:17117608-17117627 Msa0492930:intron 50.0%
CGGGCCCCTATAATACACAA+TGG + chr3_4:17118166-17118185 Msa0492930:intron 50.0%
!! CTAAGGCTTTGTTTGCGGGT+TGG + chr3_4:17118252-17118271 Msa0492930:intron 50.0%
!!! TTTGCGGGTTGGGTTTTGGA+GGG + chr3_4:17118263-17118282 Msa0492930:intron 50.0%
GGAGGGAAAGGAAAGGCTTA+TGG + chr3_4:17118290-17118309 Msa0492930:intron 50.0%
GGGAAAACCTTCCCCTTGTT+TGG - chr3_4:17118606-17118625 None:intergenic 50.0%
GGAAAGGAAAGGCTAAGGAG+GGG - chr3_4:17118661-17118680 None:intergenic 50.0%
GGGAAGGAAAGGAAAGGCTA+AGG - chr3_4:17118666-17118685 None:intergenic 50.0%
TGATCAGTTGACCCTCAGAG+AGG - chr3_4:17119972-17119991 None:intergenic 50.0%
! TTTTCTTCCAACCGGCATGG+AGG - chr3_4:17120298-17120317 None:intergenic 50.0%
!! GGTACCGATCTTGCATCAAG+TGG + chr3_4:17120330-17120349 Msa0492930:three_prime_UTR 50.0%
! TGGGGGTAAAGTGGCCATTT+TGG + chr3_4:17120501-17120520 Msa0492930:three_prime_UTR 50.0%
AGTCAAGTGTTCGCAGCCAC+GGG - chr3_4:17117433-17117452 None:intergenic 55.0%
!!! GTTTGCGGGTTGGGTTTTGG+AGG + chr3_4:17118262-17118281 Msa0492930:intron 55.0%
!!! TTGCGGGTTGGGTTTTGGAG+GGG + chr3_4:17118264-17118283 Msa0492930:intron 55.0%
! TCCCCCAAATTGGGGGGTTT+TGG + chr3_4:17118401-17118420 Msa0492930:intron 55.0%
CCCAAAACCCCCCAATTTGG+GGG - chr3_4:17118405-17118424 None:intergenic 55.0%
! CCCCCAAATTGGGGGGTTTT+GGG + chr3_4:17118402-17118421 Msa0492930:intron 55.0%
CCCCAAAACCCCCCAATTTG+GGG - chr3_4:17118406-17118425 None:intergenic 55.0%
! CCCCAAATTGGGGGGTTTTG+GGG + chr3_4:17118403-17118422 Msa0492930:intron 55.0%
CCCCCAAAACCCCCCAATTT+GGG - chr3_4:17118407-17118426 None:intergenic 55.0%
!! CCCAAATTGGGGGGTTTTGG+GGG + chr3_4:17118404-17118423 Msa0492930:intron 55.0%
!!! CCAAATTGGGGGGTTTTGGG+GGG + chr3_4:17118405-17118424 Msa0492930:intron 55.0%
GAAAGGGAGGGAAGGGAGAA+AGG - chr3_4:17118630-17118649 None:intergenic 55.0%
AGGGAAGGAAAGGGAGGGAA+GGG - chr3_4:17118637-17118656 None:intergenic 55.0%
AAGGGAAGGAAAGGGAGGGA+AGG - chr3_4:17118638-17118657 None:intergenic 55.0%
AGGAGGGGAAGGGAAGGAAA+GGG - chr3_4:17118646-17118665 None:intergenic 55.0%
AAGGAGGGGAAGGGAAGGAA+AGG - chr3_4:17118647-17118666 None:intergenic 55.0%
GGAAAGGCTAAGGAGGGGAA+GGG - chr3_4:17118656-17118675 None:intergenic 55.0%
AGGAAAGGCTAAGGAGGGGA+AGG - chr3_4:17118657-17118676 None:intergenic 55.0%
GGCCCTATAATTAGGGACGG+AGG + chr3_4:17119444-17119463 Msa0492930:intron 55.0%
!!! GGGTTGGGTTTTGGAGGGGA+GGG + chr3_4:17118268-17118287 Msa0492930:intron 60.0%
!!! GGTTGGGTTTTGGAGGGGAG+GGG + chr3_4:17118269-17118288 Msa0492930:intron 60.0%
CCCCCCAAAACCCCCCAATT+TGG - chr3_4:17118408-17118427 None:intergenic 60.0%
GGGAGGGAAGGGAGAAAGGA+GGG - chr3_4:17118626-17118645 None:intergenic 60.0%
AGGGAGGGAAGGGAGAAAGG+AGG - chr3_4:17118627-17118646 None:intergenic 60.0%
GGGGAAGGGAAGGAAAGGGA+GGG - chr3_4:17118642-17118661 None:intergenic 60.0%
AGGGGAAGGGAAGGAAAGGG+AGG - chr3_4:17118643-17118662 None:intergenic 60.0%
AGGCTAAGGAGGGGAAGGGA+AGG - chr3_4:17118652-17118671 None:intergenic 60.0%
TCAGAGACCTCCATGCCGGT+TGG + chr3_4:17120288-17120307 Msa0492930:three_prime_UTR 60.0%
!!! CGGGTTGGGTTTTGGAGGGG+AGG + chr3_4:17118267-17118286 Msa0492930:intron 65.0%
!!! TGGGTTTTGGAGGGGAGGGG+AGG + chr3_4:17118272-17118291 Msa0492930:intron 65.0%
!!! GGGTTTTGGAGGGGAGGGGA+GGG + chr3_4:17118273-17118292 Msa0492930:intron 65.0%
TTGGAGGGGAGGGGAGGGAA+AGG + chr3_4:17118278-17118297 Msa0492930:intron 65.0%
GGGGAGGGGAGGGAAAGGAA+AGG + chr3_4:17118283-17118302 Msa0492930:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 17117337 17120691 17117337 ID=Msa0492930;Name=Msa0492930
chr3_4 mRNA 17117337 17120691 17117337 ID=Msa0492930-mRNA-1;Parent=Msa0492930;Name=Msa0492930-mRNA-1;_AED=0.37;_eAED=0.43;_QI=0|0|0.5|1|1|1|2|617|67
chr3_4 exon 17117337 17117412 17117337 ID=Msa0492930-mRNA-1:exon:4850;Parent=Msa0492930-mRNA-1
chr3_4 exon 17119947 17120691 17119947 ID=Msa0492930-mRNA-1:exon:4851;Parent=Msa0492930-mRNA-1
chr3_4 CDS 17117337 17117412 17117337 ID=Msa0492930-mRNA-1:cds;Parent=Msa0492930-mRNA-1
chr3_4 CDS 17119947 17120074 17119947 ID=Msa0492930-mRNA-1:cds;Parent=Msa0492930-mRNA-1
chr3_4 three_prime_UTR 17120075 17120691 17120075 ID=Msa0492930-mRNA-1:three_prime_utr;Parent=Msa0492930-mRNA-1
Gene Sequence

>Msa0492930

ATGCTGCCAGAAAAGCATTTGGCATATGGTAGAAGTTTGCCATTGCTTTTATTTATTTCTCGAGCCTTTCCAACCGCCACATCCTCTCTGATTAAAATCCTCTCTGAGGGTCAACTGATCATACTTGTTGGAGCTAGCACTGGTAATAATGATGGAACTGAAAGGAAAAAGGAGGTCACTGTGAAGGACTATTTGGCTGAGTAA
Protein sequence

>Msa0492930

MLPEKHLAYGRSLPLLLFISRAFPTATSSLIKILSEGQLIILVGASTGNNDGTERKKEVTVKDYLAE*