AlfalfaGEDB | Msa0575320

M. sativa cultivar zhongmu-4 / Msa0575320

KEGG: -
GO: -
PFAM: PF04654.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0575320	XP_003631042.1	96.567	233	8	0	1	233	1	233	8.47e-165	464

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0575320	G7LCF1	96.567	233	8	0	1	233	1	233	4.05e-165	464

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000050	Msa0575320	0.803526	3.343219e-49	-8.615850e-47
Msa0030160	Msa0575320	0.804910	1.721407e-49	-8.615850e-47
Msa0030700	Msa0575320	0.827103	1.882436e-54	-8.615850e-47
Msa0078120	Msa0575320	0.824756	6.793147e-54	-8.615850e-47
Msa0081020	Msa0575320	0.832763	7.851196e-56	-8.615850e-47
Msa0105410	Msa0575320	0.814046	1.880677e-51	-8.615850e-47
Msa0125650	Msa0575320	0.805364	1.383331e-49	-8.615850e-47
Msa0189600	Msa0575320	0.815032	1.137875e-51	-8.615850e-47
Msa0244640	Msa0575320	0.806645	7.439421e-50	-8.615850e-47
Msa0269970	Msa0575320	0.820084	8.266818e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0575320	MtrunA17_Chr8g0392461	96.567	233	8	0	1	233	1	233	7.79e-169	464
Msa0575320	MtrunA17_Chr8g0392471	69.061	181	51	2	54	230	98	277	1.97e-80	242
Msa0575320	MtrunA17_Chr8g0392471	72.840	81	18	1	154	230	1	81	1.21e-34	125
Msa0575320	MtrunA17_Chr5g0428761	56.109	221	85	4	1	210	1	220	1.39e-79	239
Msa0575320	MtrunA17_Chr3g0139261	39.906	213	121	2	1	206	1	213	9.77e-45	149
Msa0575320	MtrunA17_Chr1g0188581	38.942	208	120	2	6	206	8	215	3.35e-43	145
Msa0575320	MtrunA17_Chr1g0147511	38.350	206	122	2	6	206	8	213	2.46e-42	143
Msa0575320	MtrunA17_Chr8g0392481	71.910	89	25	0	4	92	5	93	1.30e-41	137
Msa0575320	MtrunA17_Chr6g0477471	35.681	213	130	2	1	206	1	213	3.38e-37	130
Msa0575320	MtrunA17_Chr2g0282971	35.233	193	110	5	38	223	1	185	7.47e-31	112

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0575320	AT3G18215.1	60.169	236	87	2	4	232	5	240	1.41e-100	292
Msa0575320	AT5G24600.1	56.054	223	83	3	1	208	1	223	1.78e-77	234
Msa0575320	AT5G24600.2	56.054	223	83	3	1	208	1	223	2.82e-77	233
Msa0575320	AT3G18215.2	60.119	168	60	2	72	232	1	168	3.43e-65	200
Msa0575320	AT4G31330.1	38.356	219	122	4	1	206	1	219	4.97e-40	138
Msa0575320	AT5G10580.3	36.449	214	123	3	6	206	8	221	1.27e-36	129
Msa0575320	AT5G10580.1	36.449	214	123	3	6	206	8	221	1.59e-36	129
Msa0575320	AT5G10580.4	36.449	214	123	3	6	206	8	221	1.59e-36	129
Msa0575320	AT5G46060.1	35.622	233	140	3	6	232	10	238	1.97e-34	124
Msa0575320	AT5G24790.1	32.218	239	150	5	1	227	1	239	2.98e-29	110

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Find 50 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAGAACAAATAAACAT+TGG	0.604030	4_1:+79187397	None:intergenic
AAACCAATAACAGTGCTAGA+AGG	0.374290	4_1:+79189350	None:intergenic
AACAACACCAATATACCAAC+AGG	0.469092	4_1:+79189413	None:intergenic
AACCAATAACAGTGCTAGAA+GGG	0.540211	4_1:+79189351	None:intergenic
AACTATCCGCAACAATATAA+TGG	0.386563	4_1:-79187770	Msa0575320:CDS
AAGATCCCTTGTGATTTGAG+TGG	0.602812	4_1:+79187342	None:intergenic
AAGGTTACTGAGTGAGTGAG+TGG	0.690599	4_1:-79189549	Msa0575320:five_prime_UTR
ACATCATGAAAAGAGTCCAC+TGG	0.623254	4_1:+79189309	None:intergenic
ACATTAAGTCCTTCAGGGAG+AGG	0.548609	4_1:-79187318	Msa0575320:CDS
ACCTGCACTCAAAGGCAAAA+AGG	0.351627	4_1:-79187533	Msa0575320:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAGTGGTGAAAAATTAAGAA+AGG	-	chr4_1:79187400-79187419	Msa0575320:CDS	30.0%
GAAGATGGAAATAAAGGATT+TGG	-	chr4_1:79187482-79187501	Msa0575320:CDS	30.0%
TTTGATTCCTGTTGGTATAT+TGG	-	chr4_1:79187512-79187531	Msa0575320:CDS	30.0%
GTCAACAGATAAAAACAAGA+AGG	+	chr4_1:79187672-79187691	None:intergenic	30.0%
ACAATAACTAACATGAAGTG+TGG	+	chr4_1:79187762-79187781	None:intergenic	30.0%
TAAAGGTCAAAACTGAGAAT+TGG	-	chr4_1:79187884-79187903	Msa0575320:intron	30.0%
TGTATGTGAAACAATCAAGT+AGG	-	chr4_1:79187907-79187926	Msa0575320:intron	30.0%
ACCCAAAAATATGTGTGAAA+AGG	+	chr4_1:79187991-79188010	None:intergenic	30.0%
TATCTATGTGTATGATTGGT+TGG	-	chr4_1:79188167-79188186	Msa0575320:intron	30.0%
TGATGCCTTTGACAAAATTA+CGG	+	chr4_1:79188227-79188246	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	79187148	79189806	79187148	ID=Msa0575320;Name=Msa0575320
chr4_1	mRNA	79187148	79189806	79187148	ID=Msa0575320-mRNA-1;Parent=Msa0575320;Name=Msa0575320-mRNA-1;_AED=0.39;_eAED=0.39;_QI=338\|1\|1\|1\|1\|1\|2\|137\|233
chr4_1	exon	79187148	79187824	79187148	ID=Msa0575320-mRNA-1:exon:28237;Parent=Msa0575320-mRNA-1
chr4_1	exon	79189307	79189806	79189307	ID=Msa0575320-mRNA-1:exon:28236;Parent=Msa0575320-mRNA-1
chr4_1	five_prime_UTR	79189469	79189806	79189469	ID=Msa0575320-mRNA-1:five_prime_utr;Parent=Msa0575320-mRNA-1
chr4_1	CDS	79189307	79189468	79189307	ID=Msa0575320-mRNA-1:cds;Parent=Msa0575320-mRNA-1
chr4_1	CDS	79187285	79187824	79187285	ID=Msa0575320-mRNA-1:cds;Parent=Msa0575320-mRNA-1
chr4_1	three_prime_UTR	79187148	79187284	79187148	ID=Msa0575320-mRNA-1:three_prime_utr;Parent=Msa0575320-mRNA-1

Gene Sequence

>Msa0575320

ATGGAAATAAAGGATTTGGATTATGTTTTGATTCCTGTTGGTATATTGGTGTTGTTAATTTATCATGTTTGGCTTCTCTACACTATAATCCGACACCCTTCTAGCACTGTTATTGGTTTGAATGCTCAGTCTCGTTACCAGTGGACTCTTTTCATGATGTCTGATCCAATGAAAAATGGGGTATTGGCTGTTCAAACTATCCGCAACAATATAATGGCATCTACTCTACTAGCTACAACAGCAATCACACTAAGTTCACTAATTGGAGTTTTTGCAAGCAATGAGACAGAGACCAAATTAGTTTTTGGCAACAAAACTTCCCTTAATTCTTCAATAAAACGTCTTTCTATCTCGTTATGCTTCCTTGTTGCATTTCTATGTAATATGCAGTCTATAAGATACTATGCACATGTGAGTTTCTTAATCAATACACCTGCACTCAAAGGCAAAAAGGATTTTATTGAATATGTTGCAAAAACATTGAACCGTGGAAGTTATTCTTGGTCTCTTGGTTTGAGGGCTTTCTACACGTCAATTCCACTTGTCCTATGGATTTATGGGCCAATACCAATGTTTATTTGTTCTTGTTTCACATCATTTATTTTGTACTTCTTGGATACAACCACTCAAATCACAAGGGATCTTCACATTAAGTCCTTCAGGGAGAGGGAAAGTACTGAGGAAGTGGATGCAGCAGCATAG

Protein sequence

>Msa0575320

MEIKDLDYVLIPVGILVLLIYHVWLLYTIIRHPSSTVIGLNAQSRYQWTLFMMSDPMKNGVLAVQTIRNNIMASTLLATTAITLSSLIGVFASNETETKLVFGNKTSLNSSIKRLSISLCFLVAFLCNMQSIRYYAHVSFLINTPALKGKKDFIEYVAKTLNRGSYSWSLGLRAFYTSIPLVLWIYGPIPMFICSCFTSFILYFLDTTTQITRDLHIKSFRERESTEEVDAAA*