AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0575320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0575320 MtrunA17_Chr8g0392461 96.567 233 8 0 1 233 1 233 7.79e-169 464
Msa0575320 MtrunA17_Chr8g0392471 69.061 181 51 2 54 230 98 277 1.97e-80 242
Msa0575320 MtrunA17_Chr8g0392471 72.840 81 18 1 154 230 1 81 1.21e-34 125
Msa0575320 MtrunA17_Chr5g0428761 56.109 221 85 4 1 210 1 220 1.39e-79 239
Msa0575320 MtrunA17_Chr3g0139261 39.906 213 121 2 1 206 1 213 9.77e-45 149
Msa0575320 MtrunA17_Chr1g0188581 38.942 208 120 2 6 206 8 215 3.35e-43 145
Msa0575320 MtrunA17_Chr1g0147511 38.350 206 122 2 6 206 8 213 2.46e-42 143
Msa0575320 MtrunA17_Chr8g0392481 71.910 89 25 0 4 92 5 93 1.30e-41 137
Msa0575320 MtrunA17_Chr6g0477471 35.681 213 130 2 1 206 1 213 3.38e-37 130
Msa0575320 MtrunA17_Chr2g0282971 35.233 193 110 5 38 223 1 185 7.47e-31 112
Msa0575320 MtrunA17_Chr6g0477661 33.495 206 132 1 6 206 8 213 3.09e-29 109
Msa0575320 MtrunA17_Chr6g0477681 32.018 228 139 4 6 227 8 225 1.46e-25 100
Msa0575320 MtrunA17_Chr7g0244961 31.472 197 123 3 6 195 44 235 1.60e-21 89.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0575320 AT3G18215.1 60.169 236 87 2 4 232 5 240 1.41e-100 292
Msa0575320 AT5G24600.1 56.054 223 83 3 1 208 1 223 1.78e-77 234
Msa0575320 AT5G24600.2 56.054 223 83 3 1 208 1 223 2.82e-77 233
Msa0575320 AT3G18215.2 60.119 168 60 2 72 232 1 168 3.43e-65 200
Msa0575320 AT4G31330.1 38.356 219 122 4 1 206 1 219 4.97e-40 138
Msa0575320 AT5G10580.3 36.449 214 123 3 6 206 8 221 1.27e-36 129
Msa0575320 AT5G10580.1 36.449 214 123 3 6 206 8 221 1.59e-36 129
Msa0575320 AT5G10580.4 36.449 214 123 3 6 206 8 221 1.59e-36 129
Msa0575320 AT5G46060.1 35.622 233 140 3 6 232 10 238 1.97e-34 124
Msa0575320 AT5G24790.1 32.218 239 150 5 1 227 1 239 2.98e-29 110
Msa0575320 AT5G10580.2 37.419 155 92 2 6 155 8 162 1.63e-25 99.4
Msa0575320 AT5G43180.1 29.956 227 138 3 1 206 1 227 3.21e-21 89.0

Find 50 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTTGTCCTATGGATTTA+TGG 0.072565 4_1:-79187427 Msa0575320:CDS
CACTTGTCCTATGGATTTAT+GGG 0.267343 4_1:-79187426 Msa0575320:CDS
CACCCTTCTAGCACTGTTAT+TGG 0.273016 4_1:-79189353 Msa0575320:CDS
GAAGATGGAAATAAAGGATT+TGG 0.273589 4_1:-79189450 Msa0575320:exon
TTTGATTCCTGTTGGTATAT+TGG 0.290453 4_1:-79189420 Msa0575320:CDS
GAGGAAGAAGATGGAAATAA+AGG 0.302788 4_1:-79189456 Msa0575320:exon
ATCTTCACATTAAGTCCTTC+AGG 0.309856 4_1:-79187324 Msa0575320:CDS
TTCAGTGGTTGGTAACAATA+AGG 0.315009 4_1:-79189568 Msa0575320:five_prime_UTR
ATCACACTAAGTTCACTAAT+TGG 0.318979 4_1:-79187721 Msa0575320:CDS
TTCTTGGTCTCTTGGTTTGA+GGG 0.329290 4_1:-79187467 Msa0575320:CDS
TGTTGTTAATTTATCATGTT+TGG 0.345080 4_1:-79189397 Msa0575320:CDS
ACCTGCACTCAAAGGCAAAA+AGG 0.351627 4_1:-79187533 Msa0575320:CDS
TGAACCGTGGAAGTTATTCT+TGG 0.354981 4_1:-79187483 Msa0575320:CDS
GAATAACAGTTACATATATA+TGG 0.360421 4_1:+79187260 None:intergenic
GGAAGTTATTCTTGGTCTCT+TGG 0.373865 4_1:-79187475 Msa0575320:CDS
AAACCAATAACAGTGCTAGA+AGG 0.374290 4_1:+79189350 None:intergenic
ATTCTTGGTCTCTTGGTTTG+AGG 0.377647 4_1:-79187468 Msa0575320:CDS
AACTATCCGCAACAATATAA+TGG 0.386563 4_1:-79187770 Msa0575320:CDS
CGTCAATTCCACTTGTCCTA+TGG 0.395468 4_1:-79187435 Msa0575320:CDS
GAGGGAAAGTACTGAGGAAG+TGG 0.420276 4_1:-79187299 Msa0575320:CDS
CAAAGCAAAGAGGAAGAAGA+TGG 0.451707 4_1:-79189465 Msa0575320:exon
AACAACACCAATATACCAAC+AGG 0.469092 4_1:+79189413 None:intergenic
GAACAAATAAACATTGGTAT+TGG 0.469367 4_1:+79187403 None:intergenic
AGCACTATACTTTCAGTGGT+TGG 0.518659 4_1:-79189579 Msa0575320:five_prime_UTR
GTCTTTATCTTTATTTAACT+AGG 0.519464 4_1:-79189786 Msa0575320:five_prime_UTR
TCTTCACATTAAGTCCTTCA+GGG 0.530321 4_1:-79187323 Msa0575320:CDS
CATTAAGTCCTTCAGGGAGA+GGG 0.540101 4_1:-79187317 Msa0575320:CDS
AACCAATAACAGTGCTAGAA+GGG 0.540211 4_1:+79189351 None:intergenic
ACATTAAGTCCTTCAGGGAG+AGG 0.548609 4_1:-79187318 Msa0575320:CDS
GCATAGCACTATACTTTCAG+TGG 0.554560 4_1:-79189583 Msa0575320:five_prime_UTR
GGAAGTGGATGCAGCAGCAT+AGG 0.557130 4_1:-79187284 Msa0575320:exon
ATCAATACACCTGCACTCAA+AGG 0.557800 4_1:-79187541 Msa0575320:CDS
GTTTGACAATTGACTTGAAG+AGG 0.564464 4_1:-79189493 Msa0575320:five_prime_UTR
AGTACTTTCCCTCTCCCTGA+AGG 0.568724 4_1:+79187309 None:intergenic
TAGATGCCATTATATTGTTG+CGG 0.587400 4_1:+79187764 None:intergenic
GTATTGGCCCATAAATCCAT+AGG 0.599604 4_1:+79187419 None:intergenic
AAGATCCCTTGTGATTTGAG+TGG 0.602812 4_1:+79187342 None:intergenic
AAACAAGAACAAATAAACAT+TGG 0.604030 4_1:+79187397 None:intergenic
CCATAAATCCATAGGACAAG+TGG 0.605429 4_1:+79187427 None:intergenic
TAACAGTGCTAGAAGGGTGT+CGG 0.609595 4_1:+79189357 None:intergenic
ACATCATGAAAAGAGTCCAC+TGG 0.623254 4_1:+79189309 None:intergenic
ATACAACCACTCAAATCACA+AGG 0.654100 4_1:-79187348 Msa0575320:CDS
GAGACCAAGAATAACTTCCA+CGG 0.654635 4_1:+79187479 None:intergenic
ATGCTCAGTCTCGTTACCAG+TGG 0.659913 4_1:-79189325 Msa0575320:CDS
AGAGGCAAAGCAAAGCAAAG+AGG 0.660273 4_1:-79189475 Msa0575320:five_prime_UTR
CAGGGAGAGGGAAAGTACTG+AGG 0.672783 4_1:-79187305 Msa0575320:CDS
GTGTGTGTGTGATAATGAGG+TGG 0.676728 4_1:-79189660 Msa0575320:five_prime_UTR
AAGGTTACTGAGTGAGTGAG+TGG 0.690599 4_1:-79189549 Msa0575320:five_prime_UTR
TACAACCACTCAAATCACAA+GGG 0.726346 4_1:-79187347 Msa0575320:CDS
TATTACATAGAAATGCAACA+AGG 0.733545 4_1:+79187602 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATAAATACAAAATGTTA+AGG + chr4_1:79187705-79187724 None:intergenic 15.0%
!!! TTTGTATTTATCTTTTGTTT+AGG - chr4_1:79187711-79187730 Msa0575320:CDS 15.0%
!! AAAAGTAAAAAAATATGAAG+CGG + chr4_1:79187832-79187851 None:intergenic 15.0%
!!! ATGTATAAATATGTCTTATT+GGG + chr4_1:79188148-79188167 None:intergenic 15.0%
!! TATGTATAAATATGTCTTAT+TGG + chr4_1:79188149-79188168 None:intergenic 15.0%
!!! TTTGAATTTCATCACATTTT+TGG - chr4_1:79187301-79187320 Msa0575320:CDS 20.0%
!! TGTTGTTAATTTATCATGTT+TGG - chr4_1:79187535-79187554 Msa0575320:CDS 20.0%
!!! TGTATAAATATGTCTTATTG+GGG + chr4_1:79188147-79188166 None:intergenic 20.0%
!! ACTTATGAAAACAACTTTAT+TGG + chr4_1:79188266-79188285 None:intergenic 20.0%
!!! TACTGTTTTAATTGTTTTTC+TGG - chr4_1:79188984-79189003 Msa0575320:intron 20.0%
!!! GTTTTGTTCAATTTATGTTT+TGG - chr4_1:79189006-79189025 Msa0575320:intron 20.0%
!!! TTTTTATCAAAACTGTTAGT+GGG + chr4_1:79189076-79189095 None:intergenic 20.0%
!!! TTTTTTATCAAAACTGTTAG+TGG + chr4_1:79189077-79189096 None:intergenic 20.0%
!!! TATTGCATATATTTGTTTTC+AGG - chr4_1:79189108-79189127 Msa0575320:intron 20.0%
!!! ACGTTTTATTGAAGAATTAA+GGG + chr4_1:79189290-79189309 None:intergenic 20.0%
!! AAACAAGAACAAATAAACAT+TGG + chr4_1:79189538-79189557 None:intergenic 20.0%
!!! ATCATTTATTTTGTACTTCT+TGG - chr4_1:79189561-79189580 Msa0575320:five_prime_UTR 20.0%
!! GAATAACAGTTACATATATA+TGG + chr4_1:79189675-79189694 None:intergenic 20.0%
! TCAACAGATAAAAACAAGAA+GGG + chr4_1:79187671-79187690 None:intergenic 25.0%
!!! TGTTTTTATCTGTTGACATT+TGG - chr4_1:79187675-79187694 Msa0575320:CDS 25.0%
!!! ACATATTTTTGGGTTTTAAG+TGG - chr4_1:79187997-79188016 Msa0575320:intron 25.0%
! TTGAATATATAAGAGAAGTG+AGG - chr4_1:79188058-79188077 Msa0575320:intron 25.0%
! TACATATCTATGTGTATGAT+TGG - chr4_1:79188163-79188182 Msa0575320:intron 25.0%
!!! TAGTACTAAAAAGTGTTTCA+CGG + chr4_1:79188698-79188717 None:intergenic 25.0%
! CATTGTATTCAATAGATTTC+TGG - chr4_1:79189042-79189061 Msa0575320:intron 25.0%
! TTTGTTGCCAAAAACTAATT+TGG + chr4_1:79189263-79189282 None:intergenic 25.0%
!!! GACGTTTTATTGAAGAATTA+AGG + chr4_1:79189291-79189310 None:intergenic 25.0%
! TATTACATAGAAATGCAACA+AGG + chr4_1:79189333-79189352 None:intergenic 25.0%
! GAACAAATAAACATTGGTAT+TGG + chr4_1:79189532-79189551 None:intergenic 25.0%
!!! ATTTTTGGAGTTTTGTGTTG+TGG - chr4_1:79187316-79187335 Msa0575320:CDS 30.0%
!!! TTTTTGGAGTTTTGTGTTGT+GGG - chr4_1:79187317-79187336 Msa0575320:CDS 30.0%
GAGTGGTGAAAAATTAAGAA+AGG - chr4_1:79187400-79187419 Msa0575320:CDS 30.0%
!!! GAAAGGTAAAGTTTGTTTTG+AGG - chr4_1:79187417-79187436 Msa0575320:CDS 30.0%
GAAGATGGAAATAAAGGATT+TGG - chr4_1:79187482-79187501 Msa0575320:CDS 30.0%
!!! GATTATGTTTTGATTCCTGT+TGG - chr4_1:79187504-79187523 Msa0575320:CDS 30.0%
TTTGATTCCTGTTGGTATAT+TGG - chr4_1:79187512-79187531 Msa0575320:CDS 30.0%
GTCAACAGATAAAAACAAGA+AGG + chr4_1:79187672-79187691 None:intergenic 30.0%
ACAATAACTAACATGAAGTG+TGG + chr4_1:79187762-79187781 None:intergenic 30.0%
TAAAGGTCAAAACTGAGAAT+TGG - chr4_1:79187884-79187903 Msa0575320:intron 30.0%
TGTATGTGAAACAATCAAGT+AGG - chr4_1:79187907-79187926 Msa0575320:intron 30.0%
! TGCCTTTTCACACATATTTT+TGG - chr4_1:79187986-79188005 Msa0575320:intron 30.0%
! GCCTTTTCACACATATTTTT+GGG - chr4_1:79187987-79188006 Msa0575320:intron 30.0%
ACCCAAAAATATGTGTGAAA+AGG + chr4_1:79187991-79188010 None:intergenic 30.0%
TATCTATGTGTATGATTGGT+TGG - chr4_1:79188167-79188186 Msa0575320:intron 30.0%
TGATGCCTTTGACAAAATTA+CGG + chr4_1:79188227-79188246 None:intergenic 30.0%
ATAGCCAGTTACAACATTTA+TGG + chr4_1:79188427-79188446 None:intergenic 30.0%
!! TAGTTTTCTGGAAAAACTCT+TGG - chr4_1:79188517-79188536 Msa0575320:intron 30.0%
!! AGTTTTCTGGAAAAACTCTT+GGG - chr4_1:79188518-79188537 Msa0575320:intron 30.0%
ATTAGAGATCGAGATATCAA+AGG - chr4_1:79188582-79188601 Msa0575320:intron 30.0%
TTAGAGATCGAGATATCAAA+GGG - chr4_1:79188583-79188602 Msa0575320:intron 30.0%
CCAAATTCCAATAGGTATAT+CGG + chr4_1:79188659-79188678 None:intergenic 30.0%
! CAGTTTTTCCAAATTCCAAT+AGG + chr4_1:79188667-79188686 None:intergenic 30.0%
TTGTCAAATAGATGCTATAG+CGG - chr4_1:79188722-79188741 Msa0575320:intron 30.0%
! AGTTGACATCATTGATTGAA+AGG - chr4_1:79188786-79188805 Msa0575320:intron 30.0%
! GTTGACATCATTGATTGAAA+GGG - chr4_1:79188787-79188806 Msa0575320:intron 30.0%
! TTGACATCATTGATTGAAAG+GGG - chr4_1:79188788-79188807 Msa0575320:intron 30.0%
! TTCTTTTTCTTCCTCAACAA+TGG - chr4_1:79188819-79188838 Msa0575320:intron 30.0%
! TCTTTTTCTTCCTCAACAAT+GGG - chr4_1:79188820-79188839 Msa0575320:intron 30.0%
!! AAAACTGTTAGTGGGTTTAA+TGG + chr4_1:79189068-79189087 None:intergenic 30.0%
TTTCAGGATCCAATGAAAAA+TGG - chr4_1:79189124-79189143 Msa0575320:intron 30.0%
TTCAGGATCCAATGAAAAAT+GGG - chr4_1:79189125-79189144 Msa0575320:intron 30.0%
AACTATCCGCAACAATATAA+TGG - chr4_1:79189162-79189181 Msa0575320:intron 30.0%
TAGATGCCATTATATTGTTG+CGG + chr4_1:79189171-79189190 None:intergenic 30.0%
ATCACACTAAGTTCACTAAT+TGG - chr4_1:79189211-79189230 Msa0575320:intron 30.0%
!!! ACAGAGACCAAATTAGTTTT+TGG - chr4_1:79189253-79189272 Msa0575320:intron 30.0%
!!! TTTTGGAGTTTTGTGTTGTG+GGG - chr4_1:79187318-79187337 Msa0575320:CDS 35.0%
!! TTCAGTGGTTGGTAACAATA+AGG - chr4_1:79187364-79187383 Msa0575320:CDS 35.0%
!! GTTTGACAATTGACTTGAAG+AGG - chr4_1:79187439-79187458 Msa0575320:CDS 35.0%
GAGGAAGAAGATGGAAATAA+AGG - chr4_1:79187476-79187495 Msa0575320:CDS 35.0%
AACAACACCAATATACCAAC+AGG + chr4_1:79187522-79187541 None:intergenic 35.0%
AACCAATAACAGTGCTAGAA+GGG + chr4_1:79187584-79187603 None:intergenic 35.0%
AAACCAATAACAGTGCTAGA+AGG + chr4_1:79187585-79187604 None:intergenic 35.0%
CGTATATGCTATCATTTGTC+TGG + chr4_1:79187803-79187822 None:intergenic 35.0%
!!! TTTTGGGTTTTAAGTGGAAG+TGG - chr4_1:79188003-79188022 Msa0575320:intron 35.0%
TTCAACATTTACCCATTCAG+AGG + chr4_1:79188043-79188062 None:intergenic 35.0%
! TGTATGATTGGTTGGACTAA+CGG - chr4_1:79188175-79188194 Msa0575320:intron 35.0%
AGTTCAAAGCTTGTATTCAG+TGG - chr4_1:79188460-79188479 Msa0575320:intron 35.0%
GGTTTATACAAGTAGCATAC+TGG - chr4_1:79188481-79188500 Msa0575320:intron 35.0%
AGCAATGTTAGCTATAGCAA+CGG - chr4_1:79188629-79188648 Msa0575320:intron 35.0%
CCGATATACCTATTGGAATT+TGG - chr4_1:79188656-79188675 Msa0575320:intron 35.0%
CCAATATGATAGAGTTCCTT+TGG + chr4_1:79188856-79188875 None:intergenic 35.0%
CCAAAGGAACTCTATCATAT+TGG - chr4_1:79188853-79188872 Msa0575320:intron 35.0%
! ATGAACACCAAGATTTTACG+TGG + chr4_1:79188936-79188955 None:intergenic 35.0%
TCAGGATCCAATGAAAAATG+GGG - chr4_1:79189126-79189145 Msa0575320:intron 35.0%
TCCAATGAAAAATGGGGTAT+TGG - chr4_1:79189132-79189151 Msa0575320:intron 35.0%
CACTTGTCCTATGGATTTAT+GGG - chr4_1:79189506-79189525 Msa0575320:five_prime_UTR 35.0%
ATACAACCACTCAAATCACA+AGG - chr4_1:79189584-79189603 Msa0575320:five_prime_UTR 35.0%
TACAACCACTCAAATCACAA+GGG - chr4_1:79189585-79189604 Msa0575320:five_prime_UTR 35.0%
ATCTTCACATTAAGTCCTTC+AGG - chr4_1:79189608-79189627 Msa0575320:five_prime_UTR 35.0%
TCTTCACATTAAGTCCTTCA+GGG - chr4_1:79189609-79189628 Msa0575320:five_prime_UTR 35.0%
! TGTGTGTGTGTGTGATAATG+AGG - chr4_1:79187269-79187288 Msa0575320:exon 40.0%
GCATAGCACTATACTTTCAG+TGG - chr4_1:79187349-79187368 Msa0575320:CDS 40.0%
AGCACTATACTTTCAGTGGT+TGG - chr4_1:79187353-79187372 Msa0575320:CDS 40.0%
CAAAGCAAAGAGGAAGAAGA+TGG - chr4_1:79187467-79187486 Msa0575320:CDS 40.0%
ACATCATGAAAAGAGTCCAC+TGG + chr4_1:79187626-79187645 None:intergenic 40.0%
AAACAAGAAGGGATAGAGAG+AGG + chr4_1:79187660-79187679 None:intergenic 40.0%
ATCAAGTAGGTCACTCATTG+TGG - chr4_1:79187920-79187939 Msa0575320:intron 40.0%
TCAAGTAGGTCACTCATTGT+GGG - chr4_1:79187921-79187940 Msa0575320:intron 40.0%
!!! GGTTTTAAGTGGAAGTGGTT+AGG - chr4_1:79188008-79188027 Msa0575320:intron 40.0%
! TATGTCTTATTGGGGAGCTT+GGG + chr4_1:79188139-79188158 None:intergenic 40.0%
! ATATGTCTTATTGGGGAGCT+TGG + chr4_1:79188140-79188159 None:intergenic 40.0%
! GTGTGCCGTAATTTTGTCAA+AGG - chr4_1:79188219-79188238 Msa0575320:intron 40.0%
AATCTGTCACACACAGTTAC+TGG - chr4_1:79188401-79188420 Msa0575320:intron 40.0%
CTGGCCATAAATGTTGTAAC+TGG - chr4_1:79188420-79188439 Msa0575320:intron 40.0%
TCCAGAAAACTACCAATGCT+GGG + chr4_1:79188509-79188528 None:intergenic 40.0%
TTCCAGAAAACTACCAATGC+TGG + chr4_1:79188510-79188529 None:intergenic 40.0%
CGGTATACCGATATACCTAT+TGG - chr4_1:79188649-79188668 Msa0575320:intron 40.0%
!! GCCAATACCCCATTTTTCAT+TGG + chr4_1:79189136-79189155 None:intergenic 40.0%
ATCAATACACCTGCACTCAA+AGG - chr4_1:79189391-79189410 Msa0575320:CDS 40.0%
GTTGCAAAAACATTGAACCG+TGG - chr4_1:79189436-79189455 Msa0575320:CDS 40.0%
TGAACCGTGGAAGTTATTCT+TGG - chr4_1:79189449-79189468 Msa0575320:CDS 40.0%
GAGACCAAGAATAACTTCCA+CGG + chr4_1:79189456-79189475 None:intergenic 40.0%
GGAAGTTATTCTTGGTCTCT+TGG - chr4_1:79189457-79189476 Msa0575320:exon 40.0%
! ATTCTTGGTCTCTTGGTTTG+AGG - chr4_1:79189464-79189483 Msa0575320:exon 40.0%
! TTCTTGGTCTCTTGGTTTGA+GGG - chr4_1:79189465-79189484 Msa0575320:exon 40.0%
CCATAAATCCATAGGACAAG+TGG + chr4_1:79189508-79189527 None:intergenic 40.0%
CCACTTGTCCTATGGATTTA+TGG - chr4_1:79189505-79189524 Msa0575320:five_prime_UTR 40.0%
! GTATTGGCCCATAAATCCAT+AGG + chr4_1:79189516-79189535 None:intergenic 40.0%
AAGATCCCTTGTGATTTGAG+TGG + chr4_1:79189593-79189612 None:intergenic 40.0%
! GTGTGTGTGTGATAATGAGG+TGG - chr4_1:79187272-79187291 Msa0575320:exon 45.0%
AAGGTTACTGAGTGAGTGAG+TGG - chr4_1:79187383-79187402 Msa0575320:CDS 45.0%
AGAGGCAAAGCAAAGCAAAG+AGG - chr4_1:79187457-79187476 Msa0575320:CDS 45.0%
! TAACAGTGCTAGAAGGGTGT+CGG + chr4_1:79187578-79187597 None:intergenic 45.0%
CACCCTTCTAGCACTGTTAT+TGG - chr4_1:79187579-79187598 Msa0575320:CDS 45.0%
TGAAAAGGCAGCTCAAGTGA+AGG + chr4_1:79187976-79187995 None:intergenic 45.0%
AGGTCTCATATGCCTCTGAA+TGG - chr4_1:79188028-79188047 Msa0575320:intron 45.0%
GGTCTCATATGCCTCTGAAT+GGG - chr4_1:79188029-79188048 Msa0575320:intron 45.0%
TGCTTCAAGACCACAAGAGA+GGG + chr4_1:79188089-79188108 None:intergenic 45.0%
ATGCTTCAAGACCACAAGAG+AGG + chr4_1:79188090-79188109 None:intergenic 45.0%
!!! ACCCAGCATTGGTAGTTTTC+TGG - chr4_1:79188505-79188524 Msa0575320:intron 45.0%
! CATCATTGATTGAAAGGGGC+TGG - chr4_1:79188792-79188811 Msa0575320:intron 45.0%
TCTCTTGCTTCCCATTGTTG+AGG + chr4_1:79188833-79188852 None:intergenic 45.0%
AATGGGAAGCAAGAGACCAA+AGG - chr4_1:79188837-79188856 Msa0575320:intron 45.0%
! TCCTTGTTGCTTCGTCTCTA+AGG - chr4_1:79188905-79188924 Msa0575320:intron 45.0%
GGCTGAACCACGTAAAATCT+TGG - chr4_1:79188926-79188945 Msa0575320:intron 45.0%
ACCTGCACTCAAAGGCAAAA+AGG - chr4_1:79189399-79189418 Msa0575320:CDS 45.0%
! TCCTTTTTGCCTTTGAGTGC+AGG + chr4_1:79189403-79189422 None:intergenic 45.0%
CGTCAATTCCACTTGTCCTA+TGG - chr4_1:79189497-79189516 Msa0575320:five_prime_UTR 45.0%
ACATTAAGTCCTTCAGGGAG+AGG - chr4_1:79189614-79189633 Msa0575320:five_prime_UTR 45.0%
CATTAAGTCCTTCAGGGAGA+GGG - chr4_1:79189615-79189634 Msa0575320:five_prime_UTR 45.0%
!! TAGATAATTAAAAATATTAA+AGG - chr4_1:79187867-79187886 Msa0575320:intron 5.0%
ATGCTCAGTCTCGTTACCAG+TGG - chr4_1:79187607-79187626 Msa0575320:CDS 50.0%
GTAGGTCACTCATTGTGGGA+CGG - chr4_1:79187925-79187944 Msa0575320:intron 50.0%
AGCATACTGGAACCCAGCAT+TGG - chr4_1:79188494-79188513 Msa0575320:intron 50.0%
! CATTGATTGAAAGGGGCTGG+AGG - chr4_1:79188795-79188814 Msa0575320:intron 50.0%
GCCTTAGAGACGAAGCAACA+AGG + chr4_1:79188909-79188928 None:intergenic 50.0%
AGTACTTTCCCTCTCCCTGA+AGG + chr4_1:79189626-79189645 None:intergenic 50.0%
!! GAGGGAAAGTACTGAGGAAG+TGG - chr4_1:79189633-79189652 Msa0575320:five_prime_UTR 50.0%
!! AGAGTGTCGGGAGCAATAGC+AGG + chr4_1:79188116-79188135 None:intergenic 55.0%
GCAGCAGTAGTGCTGCATAC+TGG - chr4_1:79188334-79188353 Msa0575320:intron 55.0%
!! CAGGGAGAGGGAAAGTACTG+AGG - chr4_1:79189627-79189646 Msa0575320:five_prime_UTR 55.0%
GGAAGTGGATGCAGCAGCAT+AGG - chr4_1:79189648-79189667 Msa0575320:five_prime_UTR 55.0%
GGTCACTCATTGTGGGACGG+AGG - chr4_1:79187928-79187947 Msa0575320:intron 60.0%
! TGAGGCGTCTCCCTCTCTTG+TGG - chr4_1:79188076-79188095 Msa0575320:intron 60.0%
! TGGGGAGCTTGGGAGAGTGT+CGG + chr4_1:79188129-79188148 None:intergenic 60.0%
!! GGGGAGCTTGGGAGAGTGTC+GGG + chr4_1:79188128-79188147 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 79187148 79189806 79187148 ID=Msa0575320;Name=Msa0575320
chr4_1 mRNA 79187148 79189806 79187148 ID=Msa0575320-mRNA-1;Parent=Msa0575320;Name=Msa0575320-mRNA-1;_AED=0.39;_eAED=0.39;_QI=338|1|1|1|1|1|2|137|233
chr4_1 exon 79187148 79187824 79187148 ID=Msa0575320-mRNA-1:exon:28237;Parent=Msa0575320-mRNA-1
chr4_1 exon 79189307 79189806 79189307 ID=Msa0575320-mRNA-1:exon:28236;Parent=Msa0575320-mRNA-1
chr4_1 five_prime_UTR 79189469 79189806 79189469 ID=Msa0575320-mRNA-1:five_prime_utr;Parent=Msa0575320-mRNA-1
chr4_1 CDS 79189307 79189468 79189307 ID=Msa0575320-mRNA-1:cds;Parent=Msa0575320-mRNA-1
chr4_1 CDS 79187285 79187824 79187285 ID=Msa0575320-mRNA-1:cds;Parent=Msa0575320-mRNA-1
chr4_1 three_prime_UTR 79187148 79187284 79187148 ID=Msa0575320-mRNA-1:three_prime_utr;Parent=Msa0575320-mRNA-1
Gene Sequence

>Msa0575320

ATGGAAATAAAGGATTTGGATTATGTTTTGATTCCTGTTGGTATATTGGTGTTGTTAATTTATCATGTTTGGCTTCTCTACACTATAATCCGACACCCTTCTAGCACTGTTATTGGTTTGAATGCTCAGTCTCGTTACCAGTGGACTCTTTTCATGATGTCTGATCCAATGAAAAATGGGGTATTGGCTGTTCAAACTATCCGCAACAATATAATGGCATCTACTCTACTAGCTACAACAGCAATCACACTAAGTTCACTAATTGGAGTTTTTGCAAGCAATGAGACAGAGACCAAATTAGTTTTTGGCAACAAAACTTCCCTTAATTCTTCAATAAAACGTCTTTCTATCTCGTTATGCTTCCTTGTTGCATTTCTATGTAATATGCAGTCTATAAGATACTATGCACATGTGAGTTTCTTAATCAATACACCTGCACTCAAAGGCAAAAAGGATTTTATTGAATATGTTGCAAAAACATTGAACCGTGGAAGTTATTCTTGGTCTCTTGGTTTGAGGGCTTTCTACACGTCAATTCCACTTGTCCTATGGATTTATGGGCCAATACCAATGTTTATTTGTTCTTGTTTCACATCATTTATTTTGTACTTCTTGGATACAACCACTCAAATCACAAGGGATCTTCACATTAAGTCCTTCAGGGAGAGGGAAAGTACTGAGGAAGTGGATGCAGCAGCATAG
Protein sequence

>Msa0575320

MEIKDLDYVLIPVGILVLLIYHVWLLYTIIRHPSSTVIGLNAQSRYQWTLFMMSDPMKNGVLAVQTIRNNIMASTLLATTAITLSSLIGVFASNETETKLVFGNKTSLNSSIKRLSISLCFLVAFLCNMQSIRYYAHVSFLINTPALKGKKDFIEYVAKTLNRGSYSWSLGLRAFYTSIPLVLWIYGPIPMFICSCFTSFILYFLDTTTQITRDLHIKSFRERESTEEVDAAA*