AlfalfaGEDB | Msa0030700

M. sativa cultivar zhongmu-4 / Msa0030700

KEGG: -
GO: GO:0003002 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0007389 GO:0008150 GO:0009888 GO:0010051 GO:0010087 GO:0010089 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043621 GO:0044424 GO:0044464 GO:0048856
PFAM: PF03479.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0030700	sp\|Q9SB31\|AHL3_ARATH	67.925	212	58	5	48	256	85	289	1.90e-83	261

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000050	Msa0030700	0.811929	5.475678e-51	-8.615850e-47
Msa0001550	Msa0030700	-0.801857	7.386641e-49	-8.615850e-47
Msa0005380	Msa0030700	0.809914	1.494982e-50	-8.615850e-47
Msa0008210	Msa0030700	0.804219	2.399011e-49	-8.615850e-47
Msa0009640	Msa0030700	0.805504	1.292736e-49	-8.615850e-47
Msa0012200	Msa0030700	0.813749	2.186481e-51	-8.615850e-47
Msa0017670	Msa0030700	0.810520	1.106326e-50	-8.615850e-47
Msa0025720	Msa0030700	0.801479	8.829960e-49	-8.615850e-47
Msa0030700	Msa0057380	0.809258	2.067591e-50	-8.615850e-47
Msa0030700	Msa0060670	0.804478	2.119155e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0030700	MtrunA17_Chr1g0188211	99.412	340	2	0	1	340	1	340	0.0	685
Msa0030700	MtrunA17_Chr6g0476061	53.644	343	115	13	1	325	1	317	6.89e-91	274
Msa0030700	MtrunA17_Chr7g0245841	49.107	336	124	11	1	310	1	315	2.08e-76	238
Msa0030700	MtrunA17_Chr4g0056761	51.799	278	90	8	17	267	17	277	5.78e-63	203
Msa0030700	MtrunA17_Chr8g0351761	41.096	365	133	9	1	310	1	338	5.55e-60	196
Msa0030700	MtrunA17_Chr1g0179461	39.871	311	145	11	49	340	69	356	5.32e-50	171
Msa0030700	MtrunA17_Chr5g0398621	44.151	265	98	9	14	264	3	231	5.00e-48	162
Msa0030700	MtrunA17_Chr8g0349091	42.578	256	120	8	49	301	89	320	1.92e-43	152
Msa0030700	MtrunA17_Chr5g0397551	43.363	226	104	7	37	261	59	261	1.46e-42	150
Msa0030700	MtrunA17_Chr7g0247431	41.026	234	116	4	48	278	88	302	1.53e-42	150

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0030700	AT4G25320.1	67.925	212	58	5	48	256	85	289	1.93e-84	261
Msa0030700	AT5G62260.1	64.545	220	64	7	49	261	76	288	1.69e-83	259
Msa0030700	AT5G62260.3	64.545	220	64	7	49	261	76	288	1.69e-83	259
Msa0030700	AT5G62260.2	62.150	214	67	7	49	255	76	282	4.49e-77	238
Msa0030700	AT4G12080.1	58.051	236	73	7	48	271	88	309	1.62e-75	236
Msa0030700	AT5G51590.1	59.016	244	63	6	34	251	64	296	3.08e-71	228
Msa0030700	AT4G22770.2	56.195	226	80	7	48	267	71	283	1.32e-58	192
Msa0030700	AT4G22770.1	56.195	226	80	7	48	267	71	283	1.32e-58	192
Msa0030700	AT4G00200.2	53.817	262	91	8	15	265	14	256	1.70e-56	186
Msa0030700	AT4G00200.1	53.817	262	91	8	15	265	14	256	1.70e-56	186

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Find 122 sgRNAs with CRISPR-Local

Find 320 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGTTTGAATCTTCAAT+GGG	0.476944	1_1:+57099485	Msa0030700:five_prime_UTR
AAAATGGTTCACGTCCGTCT+TGG	0.503428	1_1:-57098957	None:intergenic
AAAATTATGTCCTCCCAACA+AGG	0.612526	1_1:+57102608	Msa0030700:CDS
AAAATTGAAAGTATAAATTT+TGG	0.212639	1_1:-57098985	None:intergenic
AAACAGCAAGAAGCAGAGGG+TGG	0.602793	1_1:+57103288	Msa0030700:CDS
AAATAAAGTAATTAAATATT+CGG	0.279604	1_1:-57099038	None:intergenic
AACATTTGAAATTGTGCCAG+TGG	0.637698	1_1:-57102657	None:intergenic
AACGGAATCGACGATGAAAT+CGG	0.441018	1_1:-57099793	None:intergenic
AACGGTGCCGGTGTCGTTGC+CGG	0.338330	1_1:+57099678	Msa0030700:CDS
AACTTACCAGGACAGAATTG+TGG	0.576879	1_1:+57103584	Msa0030700:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTGTTCCAATTCAATTTCT+AGG	+	chr1_1:57099301-57099320	Msa0030700:five_prime_UTR	30.0%
TGAAGACCTAGAAATTGAAT+TGG	-	chr1_1:57099310-57099329	None:intergenic	30.0%
TTACTTCAGAAAACAGAGTT+TGG	-	chr1_1:57099403-57099422	None:intergenic	30.0%
GAAAACGTTTGAATCTTCAA+TGG	+	chr1_1:57099484-57099503	Msa0030700:five_prime_UTR	30.0%
GATTTCTTCATAGATTCAAC+AGG	-	chr1_1:57099859-57099878	None:intergenic	30.0%
CTATAAATAATACCTTGCAC+AGG	-	chr1_1:57099910-57099929	None:intergenic	30.0%
CTTTGTATTCAGATCTGAAA+AGG	-	chr1_1:57100321-57100340	None:intergenic	30.0%
AGGTTGTATTTGTTCTGTTT+AGG	+	chr1_1:57100378-57100397	Msa0030700:intron	30.0%
GGTTGTATTTGTTCTGTTTA+GGG	+	chr1_1:57100379-57100398	Msa0030700:intron	30.0%
GCATTATATGACAATGGAAA+TGG	+	chr1_1:57100450-57100469	Msa0030700:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	57098957	57103876	57098957	ID=Msa0030700;Name=Msa0030700
chr1_1	mRNA	57098957	57103876	57098957	ID=Msa0030700-mRNA-1;Parent=Msa0030700;Name=Msa0030700-mRNA-1;_AED=0.06;_eAED=0.06;_QI=611\|1\|1\|1\|1\|1\|5\|333\|340
chr1_1	exon	57098957	57099916	57098957	ID=Msa0030700-mRNA-1:exon:17199;Parent=Msa0030700-mRNA-1
chr1_1	exon	57102341	57102426	57102341	ID=Msa0030700-mRNA-1:exon:17200;Parent=Msa0030700-mRNA-1
chr1_1	exon	57102596	57102724	57102596	ID=Msa0030700-mRNA-1:exon:17201;Parent=Msa0030700-mRNA-1
chr1_1	exon	57102993	57103154	57102993	ID=Msa0030700-mRNA-1:exon:17202;Parent=Msa0030700-mRNA-1
chr1_1	exon	57103247	57103876	57103247	ID=Msa0030700-mRNA-1:exon:17203;Parent=Msa0030700-mRNA-1
chr1_1	five_prime_UTR	57098957	57099567	57098957	ID=Msa0030700-mRNA-1:five_prime_utr;Parent=Msa0030700-mRNA-1
chr1_1	CDS	57099568	57099916	57099568	ID=Msa0030700-mRNA-1:cds;Parent=Msa0030700-mRNA-1
chr1_1	CDS	57102341	57102426	57102341	ID=Msa0030700-mRNA-1:cds;Parent=Msa0030700-mRNA-1
chr1_1	CDS	57102596	57102724	57102596	ID=Msa0030700-mRNA-1:cds;Parent=Msa0030700-mRNA-1
chr1_1	CDS	57102993	57103154	57102993	ID=Msa0030700-mRNA-1:cds;Parent=Msa0030700-mRNA-1
chr1_1	CDS	57103247	57103543	57103247	ID=Msa0030700-mRNA-1:cds;Parent=Msa0030700-mRNA-1
chr1_1	three_prime_UTR	57103544	57103876	57103544	ID=Msa0030700-mRNA-1:three_prime_utr;Parent=Msa0030700-mRNA-1

Gene Sequence

>Msa0030700

ATGGAAGAAAGAGAGAATTTTGGTGCTGTGAATGTAGTGGGTGGTAATGAGGTTCCTGTGAGCTTTCAAGCTGCTCCAAGGATTGATAACAACTTGGATTTTTCCATGGCAACGGTGCCGGTGTCGTTGCCGGAGACAGCTTTGAAGAAGAAGAGGGGAAGACCAAGGAAGTATGGTCCAGATGGTAAGCCTGCTCCTGGAGCTGTGACTGCGCTTTCGCCTATGCCGATTTCATCGTCGATTCCGTTGACTGGAGAGTTCTCAGCTTGGAAAAGGGGTAGAGGGAAGCCTGTTGAATCTATGAAGAAATCATCTTTTAAGTTTGATTTTGAAAGTCCACCTGTGCAAGTTGTAGGTGGAGGTGTAAGTGAGGGAATTGCATACTCAGTAGGTGCCAACTTCACAGCTTATGTGCTTACAGTGAATTCTGGCGAGGATGTTACCATGAAAATTATGTCCTCCCAACAAGGATCACGTGCAATATGTATTCTCTCTGCCACTGGCACAATTTCAAATGTTACACTTCGTCAATCGACTTCTTCAGGGGGTACCTTAACGTATGAGGGGCGGTTTGAGATTCTTTCCTTGTCTGGTTCCTTTATGCCAACTGAGAATGGAATTACAAGAAGCAGATCTGGTGGGATGAGTGTCTCTTTGGCAGGCCCAGATGGCCGAGTATTGGGTGGTGGACTCGCTGGTTTGCTTATAGCTTCCGGTCCCGTGCAGGTCGTTGTGGGAAGTTTTCTTCCTGGTCACCATCTGGAGCAAAACAGCAAGAAGCAGAGGGTGGAGCATAATATATCGACTATTACACATAATAATCCTCCTCCGCATGTTAATCATCATGTGTCTAATGAAGTAAGTTTCGGTGGAGTGAAGCCTTTTATGACACCTGCTGCATTTCAAGAAGAGAATATTGTTTCTTTCAACAACAATGCTCAAGACTCTAGGAACACCTCATCTGCTGAAGACAGAGATCCTTTACCTGAAAAGGACTCTAATCATAGTCAATCAACTACTTGA

Protein sequence

>Msa0030700

MEERENFGAVNVVGGNEVPVSFQAAPRIDNNLDFSMATVPVSLPETALKKKRGRPRKYGPDGKPAPGAVTALSPMPISSSIPLTGEFSAWKRGRGKPVESMKKSSFKFDFESPPVQVVGGGVSEGIAYSVGANFTAYVLTVNSGEDVTMKIMSSQQGSRAICILSATGTISNVTLRQSTSSGGTLTYEGRFEILSLSGSFMPTENGITRSRSGGMSVSLAGPDGRVLGGGLAGLLIASGPVQVVVGSFLPGHHLEQNSKKQRVEHNISTITHNNPPPHVNHHVSNEVSFGGVKPFMTPAAFQEENIVSFNNNAQDSRNTSSAEDRDPLPEKDSNHSQSTT*