Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0411190 | XP_013460046.1 | 87.786 | 786 | 40 | 6 | 1 | 732 | 1 | 784 | 0.0 | 1021 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0411190 | sp|Q9SUA1|DEKP3_ARATH | 46.169 | 522 | 194 | 15 | 275 | 732 | 236 | 734 | 1.16e-92 | 309 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0411190 | A0A072UWT2 | 87.786 | 786 | 40 | 6 | 1 | 732 | 1 | 784 | 0.0 | 1021 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001240 | Msa0411190 | 0.828821 | 7.264053e-55 | -8.615850e-47 |
| Msa0014990 | Msa0411190 | 0.817671 | 2.922695e-52 | -8.615850e-47 |
| Msa0017510 | Msa0411190 | 0.814590 | 1.425495e-51 | -8.615850e-47 |
| Msa0020930 | Msa0411190 | 0.870538 | 1.353115e-66 | -8.615850e-47 |
| Msa0031340 | Msa0411190 | 0.840622 | 7.782586e-58 | -8.615850e-47 |
| Msa0032110 | Msa0411190 | 0.819121 | 1.371600e-52 | -8.615850e-47 |
| Msa0044260 | Msa0411190 | 0.815918 | 7.227507e-52 | -8.615850e-47 |
| Msa0045790 | Msa0411190 | 0.808132 | 3.599329e-50 | -8.615850e-47 |
| Msa0046160 | Msa0411190 | 0.812937 | 3.297650e-51 | -8.615850e-47 |
| Msa0068680 | Msa0411190 | 0.863023 | 3.346523e-64 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0411190 | MtrunA17_Chr3g0102341 | 87.786 | 786 | 40 | 6 | 1 | 732 | 1 | 784 | 0.0 | 1021 |
| Msa0411190 | MtrunA17_Chr5g0446481 | 43.086 | 687 | 279 | 17 | 1 | 594 | 1 | 668 | 1.60e-94 | 315 |
| Msa0411190 | MtrunA17_Chr5g0446481 | 57.529 | 259 | 102 | 6 | 476 | 732 | 700 | 952 | 5.87e-54 | 201 |
| Msa0411190 | MtrunA17_Chr3g0132011 | 35.012 | 417 | 205 | 10 | 355 | 724 | 91 | 488 | 2.14e-46 | 174 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0411190 | AT4G26630.2 | 46.169 | 522 | 194 | 15 | 275 | 732 | 236 | 734 | 1.18e-93 | 309 |
| Msa0411190 | AT4G26630.1 | 46.169 | 522 | 194 | 15 | 275 | 732 | 236 | 734 | 1.18e-93 | 309 |
| Msa0411190 | AT5G55660.1 | 46.593 | 455 | 181 | 11 | 330 | 731 | 297 | 742 | 4.44e-85 | 286 |
| Msa0411190 | AT3G48710.1 | 32.697 | 419 | 196 | 10 | 355 | 724 | 53 | 434 | 2.09e-42 | 160 |
| Msa0411190 | AT5G63550.1 | 41.885 | 191 | 69 | 3 | 357 | 507 | 95 | 283 | 1.69e-33 | 136 |
| Msa0411190 | AT5G63550.2 | 41.667 | 192 | 69 | 4 | 357 | 507 | 95 | 284 | 3.93e-32 | 132 |
| Msa0411190 | AT1G64253.3 | 58.182 | 55 | 23 | 0 | 675 | 729 | 130 | 184 | 8.78e-12 | 65.1 |
| Msa0411190 | AT1G64253.2 | 58.182 | 55 | 23 | 0 | 675 | 729 | 262 | 316 | 4.18e-11 | 65.5 |
| Msa0411190 | AT1G64253.1 | 58.182 | 55 | 23 | 0 | 675 | 729 | 338 | 392 | 6.07e-11 | 65.5 |
Find 200 sgRNAs with CRISPR-Local
Find 617 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAAGAAGATAAGGCAG+AGG | 0.559965 | 3_2:+53338000 | Msa0411190:CDS |
| AAACGAGGTACACCAAGGTC+TGG | 0.545991 | 3_2:+53341172 | Msa0411190:CDS |
| AAACGCCACAGAAATCTGAA+AGG | 0.602760 | 3_2:+53343007 | Msa0411190:CDS |
| AAAGAAGAAGATAAGGCAGA+GGG | 0.612875 | 3_2:+53338001 | Msa0411190:CDS |
| AAAGACTTGGAAACTGATGG+AGG | 0.652291 | 3_2:+53337149 | Msa0411190:CDS |
| AAAGAGGAGAAGAAAGATGA+AGG | 0.475472 | 3_2:+53337479 | Msa0411190:CDS |
| AAAGAGGAGAAGAAAGATGA+TGG | 0.445699 | 3_2:+53337515 | Msa0411190:CDS |
| AAAGAGGATAAGAAAGCCGA+TGG | 0.586492 | 3_2:+53337305 | Msa0411190:CDS |
| AAAGAGGATAAGGAAGTTGA+TGG | 0.453853 | 3_2:+53337551 | Msa0411190:CDS |
| AAATGAAGATTCTTCAGGAG+TGG | 0.559104 | 3_2:+53342879 | Msa0411190:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTCATTTAGCATTAACTTCA+GGG | - | chr3_2:53336517-53336536 | None:intergenic | 30.0% | |
| ACTCATTTAGCATTAACTTC+AGG | - | chr3_2:53336518-53336537 | None:intergenic | 30.0% | |
| AAATACCCTTCTGTTGTAAA+TGG | + | chr3_2:53336844-53336863 | Msa0411190:intron | 30.0% | |
| TATCTAAGCAAGATCAGTAT+GGG | + | chr3_2:53337038-53337057 | Msa0411190:exon | 30.0% | |
| TGAAGAAGATAAGAAAGTTG+AGG | + | chr3_2:53337229-53337248 | Msa0411190:CDS | 30.0% | |
| GAAGAAGATAAGAAAGTTGA+GGG | + | chr3_2:53337230-53337249 | Msa0411190:CDS | 30.0% | |
| AGGATAAGAAAGATGAAGAT+GGG | + | chr3_2:53337270-53337289 | Msa0411190:CDS | 30.0% | |
| TGATGTGGTAAAAGTAAAAG+AGG | + | chr3_2:53337427-53337446 | Msa0411190:CDS | 30.0% | |
| GTGGAAAAGAAAGATAATGA+GGG | + | chr3_2:53337590-53337609 | Msa0411190:CDS | 30.0% | |
| TGGAAAAGAAAGATAATGAG+GGG | + | chr3_2:53337591-53337610 | Msa0411190:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 53336083 | 53345175 | 53336083 | ID=Msa0411190;Name=Msa0411190 |
| chr3_2 | mRNA | 53336083 | 53345175 | 53336083 | ID=Msa0411190-mRNA-1;Parent=Msa0411190;Name=Msa0411190-mRNA-1;_AED=0.07;_eAED=0.07;_QI=160|0.9|1|1|0.8|0.81|11|380|753 |
| chr3_2 | exon | 53336083 | 53336178 | 53336083 | ID=Msa0411190-mRNA-1:exon:10619;Parent=Msa0411190-mRNA-1 |
| chr3_2 | exon | 53336992 | 53338222 | 53336992 | ID=Msa0411190-mRNA-1:exon:10620;Parent=Msa0411190-mRNA-1 |
| chr3_2 | exon | 53338319 | 53338352 | 53338319 | ID=Msa0411190-mRNA-1:exon:10621;Parent=Msa0411190-mRNA-1 |
| chr3_2 | exon | 53339760 | 53339839 | 53339760 | ID=Msa0411190-mRNA-1:exon:10622;Parent=Msa0411190-mRNA-1 |
| chr3_2 | exon | 53340069 | 53340128 | 53340069 | ID=Msa0411190-mRNA-1:exon:10623;Parent=Msa0411190-mRNA-1 |
| chr3_2 | exon | 53340950 | 53341027 | 53340950 | ID=Msa0411190-mRNA-1:exon:10624;Parent=Msa0411190-mRNA-1 |
| chr3_2 | exon | 53341145 | 53341225 | 53341145 | ID=Msa0411190-mRNA-1:exon:10625;Parent=Msa0411190-mRNA-1 |
| chr3_2 | exon | 53342868 | 53343342 | 53342868 | ID=Msa0411190-mRNA-1:exon:10626;Parent=Msa0411190-mRNA-1 |
| chr3_2 | exon | 53343565 | 53343674 | 53343565 | ID=Msa0411190-mRNA-1:exon:10627;Parent=Msa0411190-mRNA-1 |
| chr3_2 | exon | 53343869 | 53343899 | 53343869 | ID=Msa0411190-mRNA-1:exon:10628;Parent=Msa0411190-mRNA-1 |
| chr3_2 | exon | 53344650 | 53345175 | 53344650 | ID=Msa0411190-mRNA-1:exon:10629;Parent=Msa0411190-mRNA-1 |
| chr3_2 | five_prime_UTR | 53336083 | 53336178 | 53336083 | ID=Msa0411190-mRNA-1:five_prime_utr;Parent=Msa0411190-mRNA-1 |
| chr3_2 | five_prime_UTR | 53336992 | 53337055 | 53336992 | ID=Msa0411190-mRNA-1:five_prime_utr;Parent=Msa0411190-mRNA-1 |
| chr3_2 | CDS | 53337056 | 53338222 | 53337056 | ID=Msa0411190-mRNA-1:cds;Parent=Msa0411190-mRNA-1 |
| chr3_2 | CDS | 53338319 | 53338352 | 53338319 | ID=Msa0411190-mRNA-1:cds;Parent=Msa0411190-mRNA-1 |
| chr3_2 | CDS | 53339760 | 53339839 | 53339760 | ID=Msa0411190-mRNA-1:cds;Parent=Msa0411190-mRNA-1 |
| chr3_2 | CDS | 53340069 | 53340128 | 53340069 | ID=Msa0411190-mRNA-1:cds;Parent=Msa0411190-mRNA-1 |
| chr3_2 | CDS | 53340950 | 53341027 | 53340950 | ID=Msa0411190-mRNA-1:cds;Parent=Msa0411190-mRNA-1 |
| chr3_2 | CDS | 53341145 | 53341225 | 53341145 | ID=Msa0411190-mRNA-1:cds;Parent=Msa0411190-mRNA-1 |
| chr3_2 | CDS | 53342868 | 53343342 | 53342868 | ID=Msa0411190-mRNA-1:cds;Parent=Msa0411190-mRNA-1 |
| chr3_2 | CDS | 53343565 | 53343674 | 53343565 | ID=Msa0411190-mRNA-1:cds;Parent=Msa0411190-mRNA-1 |
| chr3_2 | CDS | 53343869 | 53343899 | 53343869 | ID=Msa0411190-mRNA-1:cds;Parent=Msa0411190-mRNA-1 |
| chr3_2 | CDS | 53344650 | 53344795 | 53344650 | ID=Msa0411190-mRNA-1:cds;Parent=Msa0411190-mRNA-1 |
| chr3_2 | three_prime_UTR | 53344796 | 53345175 | 53344796 | ID=Msa0411190-mRNA-1:three_prime_utr;Parent=Msa0411190-mRNA-1 |
| Gene Sequence |
>Msa0411190 ATGGGTGAGGAAGAAGTAGTGGCTGAAGTTTCTAAGACTGATTCAAATGGAGGTAGCGTTCCGGTGAAATCATTGGGGGATGTTGTTGAGAAGAAAGACTTGGAAACTGATGGAGGGAAAGTCTTGGAGAGTAATGGAGTCAAAGAGGTGAAAGAAAATGATGTAAAAGAAATTGAAGAAGATAAGAAAGTTGAGGGTGTGGAAAAGGTACAAGAGGATAAGAAAGATGAAGATGGGGTGGGAGAAGTAAAAGAGGATAAGAAAGCCGATGGGGGCGATGAAGTAAAAGAGGATAAGAAAAATGATGGAGTGGAAGAAGTAAAAGATGAAAAGAAAGACAATGTAGCTGAAGTAAAAGAGGATAAGAAAGATGATGTGGTAAAAGTAAAAGAGGATAAGAAAGACGATGTGGTGGAAGAAGTGAAAGAGGAGAAGAAAGATGAAGGGGCTGATGAAGTAAAAGAGGAGAAGAAAGATGATGGGGTGGAAGAAGTAAAAGAGGATAAGGAAGTTGATGGTGCAGATGAAGTAAAGGTGGAAAAGAAAGATAATGAGGGGGAAGAAGTAAAAGATGATATGGAAGTTGATAGTGTGAAAGAAGCAGTGGAGGATAAGAATGATGATGACATCCAAGAAATGGAAGAGGATGACAAAAATGTTCCTGAATCTGAGAATGAGAAAATGGATGTTGATGCTGAGGTTAAGGAAACTACAGAAGATAAAGAAGAAAAGGAAAAAGAAGATGCAGAGGGTAGCATTGAGGAGAAAGAAGAGGGTAATGATGAGGAGAAAACTGAAGTAGAGGAGAAGGAAGAAGGCGATGACAATGAGAAATCTGAAGAAGAGACAGAGGAGAAGGAAGAGGGCGATGAAAAGGAGAAAACTGAAGCAGAGACGGAGGAGGAGGAGGAGGAGGAGGACGACGAGGATACTATTGATAAATCAAAAGAAGAAGATAAGGCAGAGGGTAGTAAGGCTGAGAAAGGATCAAAAAAGCGTTCCAGAGGGAAGGTTAATGAGGAGAAAGTGAAAGACAAAAAGAAGGAACTGAAGCAGCCAGAGCCAAAGACTCCAACTAGTGATCGCCCAGTGCGAGAAAGGAAATCAGTTGAGAGATTGGTAGCATTAATCGATAAAGATGCAACCAAGGAATTACACATTGCAAAGGGTCGTGGCACACCTTTAAAAGATATACCCAATGTGGCATTTAAGTTATCCAGAAGGAAGACTGATGATAGTCTCAAGTTGTTCCATACAATCCTCTTTGGAAGGAGAGGGAAGGCAGTTCAGGTCAAGAGTAATATATTGAGGTTTTCTGGTTTTGTATGGCATGACAATGAGGAAGATATTATTGCTAAGCTGACGGACTTTTTGGTTGCCCCTCATGCAACCACAACTGTGGTACTTGCAGACAAAGAGTCAAGCAAGAAGCGTAGGCGGACTGTAAAACGAGGTACACCAAGGTCTGGTGGGGCTAGTACTTCAAGAGGTTCTGGAAAGAGGCAGAAGAAAAATGAAGATTCTTCAGGAGTGGAGAAGAAGAGCACAACTGACACAGAAGATGAATCAGAGGGAGAAGAGAAAAATGAAGAAAATGAGGATGAACCTGAAAATGATATTCCTGAGAAATCTGATGATGAAACGCCACAGAAATCTGAAAGGGAAGATAAGAGTGATTCTGGGAGTGAATCTGAAGATGTGAAAAAAAGGAAAAGACCTTCCAAAACATCATCTGCAAAGAAAGAGTCCGCCGGAAGAAGCAAAACTGAGAAAACCACGGTCACAAATAAATCTCGCTCACCACCACCTAAGAGAGCACCTAAGAAATCATCAAATACCAAACCAAAGTCCGATGATGATATTGATGAAAGTCCAAAGGTATTTTCTAGGAAGAAGAAAAGTGAGCAAGGGGGAAAGCAGAAGATATCAACTCCAACTCCAACTAAGTCTTCCTCAAAGTCCAAAGAGAAAGCTGAAAAGGTTACTAAGGGAAAAGGCAAGAAGAAAGTGACATCCTCAAGTCCCACAGATGATCAGTTGCGTGATGCAATATGTGAAATTCTTAAAGAAGTTGACTTCAATACGGCCACATTTACCGACATTCTGAAACTACTTGCTAACAAATTTGATGTGGATCTCACCCCAAAAAAGTCAGCTATCAAGCTCATGATTCAGAAAGAGCTGGCAAGACTTGCTGAAGAAGCAGATGAGTCCGAGGAAGATGAAGAGGAGGATGCCGAGAAAGATGAAGACCGGTCTTAA |
| Protein sequence |
>Msa0411190 MGEEEVVAEVSKTDSNGGSVPVKSLGDVVEKKDLETDGGKVLESNGVKEVKENDVKEIEEDKKVEGVEKVQEDKKDEDGVGEVKEDKKADGGDEVKEDKKNDGVEEVKDEKKDNVAEVKEDKKDDVVKVKEDKKDDVVEEVKEEKKDEGADEVKEEKKDDGVEEVKEDKEVDGADEVKVEKKDNEGEEVKDDMEVDSVKEAVEDKNDDDIQEMEEDDKNVPESENEKMDVDAEVKETTEDKEEKEKEDAEGSIEEKEEGNDEEKTEVEEKEEGDDNEKSEEETEEKEEGDEKEKTEAETEEEEEEEDDEDTIDKSKEEDKAEGSKAEKGSKKRSRGKVNEEKVKDKKKELKQPEPKTPTSDRPVRERKSVERLVALIDKDATKELHIAKGRGTPLKDIPNVAFKLSRRKTDDSLKLFHTILFGRRGKAVQVKSNILRFSGFVWHDNEEDIIAKLTDFLVAPHATTTVVLADKESSKKRRRTVKRGTPRSGGASTSRGSGKRQKKNEDSSGVEKKSTTDTEDESEGEEKNEENEDEPENDIPEKSDDETPQKSEREDKSDSGSESEDVKKRKRPSKTSSAKKESAGRSKTEKTTVTNKSRSPPPKRAPKKSSNTKPKSDDDIDESPKVFSRKKKSEQGGKQKISTPTPTKSSSKSKEKAEKVTKGKGKKKVTSSSPTDDQLRDAICEILKEVDFNTATFTDILKLLANKFDVDLTPKKSAIKLMIQKELARLAEEADESEEDEEEDAEKDEDRS* |
