AlfalfaGEDB | Msa0854730

M. sativa cultivar zhongmu-4 / Msa0854730

KEGG: K13262|K16040
GO: GO:0003674 GO:0003824 GO:0006725 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008757 GO:0009058 GO:0009812 GO:0009987 GO:0016740 GO:0016741 GO:0019438 GO:0030761 GO:0032259 GO:0044237 GO:0044249 GO:0071704
PFAM: PF08100.14

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0854730	QGL04297.1	99.695	328	1	0	1	328	31	358	0.0	676

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0854730	sp\|B0ZB57\|OMT3_HUMLU	58.683	334	132	2	1	328	44	377	1.96e-141	407

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0854730	A0A649WZ66	99.695	328	1	0	1	328	31	358	0.0	676

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0018220	Msa0854730	0.802584	5.233394e-49	-8.615850e-47
Msa0052460	Msa0854730	0.819151	1.349734e-52	-8.615850e-47
Msa0053110	Msa0854730	0.811363	7.269266e-51	-8.615850e-47
Msa0072810	Msa0854730	0.814215	1.725745e-51	-8.615850e-47
Msa0097050	Msa0854730	0.804059	2.590521e-49	-8.615850e-47
Msa0103000	Msa0854730	0.822864	1.885682e-53	-8.615850e-47
Msa0114540	Msa0854730	-0.805975	1.029552e-49	-8.615850e-47
Msa0691160	Msa0854730	0.802826	4.665372e-49	-8.615850e-47
Msa0734980	Msa0854730	0.853305	2.608290e-61	-8.615850e-47
Msa0740420	Msa0854730	0.800960	1.128013e-48	-8.615850e-47
Msa0747780	Msa0854730	0.807217	5.631209e-50	-8.615850e-47
Msa0754030	Msa0854730	0.807450	5.023863e-50	-8.615850e-47
Msa0776910	Msa0854730	0.831461	1.647545e-55	-8.615850e-47
Msa0807110	Msa0854730	0.810654	1.035162e-50	-8.615850e-47
Msa0814110	Msa0854730	0.825303	5.044015e-54	-8.615850e-47
Msa0833040	Msa0854730	0.819728	9.973579e-53	-8.615850e-47
Msa0219190	Msa0854730	0.814960	1.180652e-51	-8.615850e-47
Msa0371540	Msa0854730	0.806159	9.417409e-50	-8.615850e-47
Msa0378340	Msa0854730	0.800926	1.146108e-48	-8.615850e-47
Msa0413930	Msa0854730	0.811803	5.830569e-51	-8.615850e-47
Msa0419970	Msa0854730	0.804951	1.687900e-49	-8.615850e-47
Msa0238890	Msa0854730	0.810152	1.328279e-50	-8.615850e-47
Msa0289890	Msa0854730	0.838316	3.091110e-57	-8.615850e-47
Msa0308830	Msa0854730	0.845906	3.031927e-59	-8.615850e-47
Msa0337850	Msa0854730	0.803473	3.427465e-49	-8.615850e-47
Msa0517340	Msa0854730	0.805411	1.352258e-49	-8.615850e-47
Msa0854730	Msa0867350	0.806281	8.874986e-50	-8.615850e-47
Msa0854730	Msa0873190	0.836643	8.299698e-57	-8.615850e-47
Msa0854730	Msa0896220	0.809483	1.850460e-50	-8.615850e-47
Msa0854730	Msa0938430	0.834262	3.317968e-56	-8.615850e-47
Msa0854730	Msa0975580	0.823174	1.596840e-53	-8.615850e-47
Msa0854730	Msa0996080	0.815493	8.985517e-52	-8.615850e-47
Msa0854730	Msa1014270	0.800953	1.131520e-48	-8.615850e-47
Msa0854730	Msa1020710	0.820215	7.714444e-53	-8.615850e-47
Msa0854730	Msa1055260	0.816543	5.237782e-52	-8.615850e-47
Msa0854730	Msa1055280	0.817973	2.496876e-52	-8.615850e-47
Msa0854730	Msa1099030	0.802115	6.539751e-49	-8.615850e-47
Msa0854730	Msa1167400	0.806975	6.335147e-50	-8.615850e-47
Msa0854730	Msa1176690	0.809378	1.948900e-50	-8.615850e-47
Msa0854730	Msa1228210	0.803366	3.606996e-49	-8.615850e-47
Msa0854730	Msa1228610	0.805771	1.136711e-49	-8.615850e-47
Msa0854730	Msa1408870	0.810937	8.992126e-51	-8.615850e-47
Msa0854730	Msa1420060	0.816268	6.036184e-52	-8.615850e-47
Msa0854730	Msa1442290	0.805139	1.542149e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0854730	MtrunA17_Chr5g0432301	82.927	328	55	1	1	327	32	359	0.0	559
Msa0854730	MtrunA17_Chr5g0432251	82.371	329	57	1	1	328	31	359	0.0	558
Msa0854730	MtrunA17_Chr5g0432211	84.802	329	35	2	1	328	31	345	0.0	557
Msa0854730	MtrunA17_Chr5g0432241	80.851	329	62	1	1	328	32	360	0.0	545
Msa0854730	MtrunA17_Chr5g0432261	69.301	329	72	3	1	328	27	327	4.37e-165	462
Msa0854730	MtrunA17_Chr4g0040621	62.424	330	118	4	1	328	44	369	1.13e-147	420
Msa0854730	MtrunA17_Chr4g0046361	49.550	333	161	6	1	328	31	361	2.83e-108	320
Msa0854730	MtrunA17_Chr4g0014681	50.746	335	156	6	1	328	31	363	2.27e-106	315
Msa0854730	MtrunA17_Chr4g0014561	50.149	335	158	6	1	328	31	363	1.19e-104	310
Msa0854730	MtrunA17_Chr4g0046341	50.000	336	158	6	1	328	31	364	9.69e-101	300
Msa0854730	MtrunA17_Chr7g0219141	45.181	332	173	6	1	328	31	357	1.85e-100	300
Msa0854730	MtrunA17_Chr4g0014641	48.358	335	164	6	1	328	31	363	3.94e-100	299
Msa0854730	MtrunA17_Chr7g0223241	44.343	327	173	5	1	326	30	348	1.63e-94	284
Msa0854730	MtrunA17_Chr7g0219161	45.000	320	167	6	1	316	31	345	2.45e-94	284
Msa0854730	MtrunA17_Chr7g0223231	43.119	327	177	5	1	326	30	348	4.10e-94	283
Msa0854730	MtrunA17_Chr4g0014661	48.036	331	165	4	1	328	31	357	3.89e-93	281
Msa0854730	MtrunA17_Chr4g0046531	45.758	330	173	5	1	328	393	718	1.02e-91	288
Msa0854730	MtrunA17_Chr4g0046531	45.897	329	172	5	1	327	31	355	1.69e-90	285
Msa0854730	MtrunA17_Chr1g0188391	44.012	334	178	7	1	328	31	361	2.52e-91	276
Msa0854730	MtrunA17_Chr4g0014591	47.432	331	167	4	1	328	31	357	1.88e-89	271
Msa0854730	MtrunA17_Chr1g0188371	43.590	312	170	5	1	308	31	340	8.83e-82	253
Msa0854730	MtrunA17_Chr3g0121301	38.700	323	192	4	3	324	40	357	1.00e-70	224
Msa0854730	MtrunA17_Chr4g0021981	38.554	332	195	7	1	328	42	368	1.70e-67	216
Msa0854730	MtrunA17_Chr4g0014601	44.444	234	123	4	1	231	31	260	4.53e-64	203
Msa0854730	MtrunA17_Chr4g0022021	37.651	332	198	6	1	328	42	368	3.68e-62	202
Msa0854730	MtrunA17_Chr7g0276411	32.515	326	198	11	1	313	31	347	1.31e-45	159
Msa0854730	MtrunA17_Chr1g0200491	29.851	335	221	7	3	327	41	371	8.40e-45	157
Msa0854730	MtrunA17_Chr8g0367441	33.648	318	197	8	1	309	46	358	2.85e-44	155
Msa0854730	MtrunA17_Chr7g0276401	32.915	319	203	8	1	313	47	360	1.39e-43	154
Msa0854730	MtrunA17_Chr3g0128011	30.864	324	206	11	1	314	88	403	2.62e-42	151
Msa0854730	MtrunA17_Chr8g0367491	30.841	321	210	6	1	313	45	361	3.31e-42	150
Msa0854730	MtrunA17_Chr8g0367451	29.851	335	223	7	1	327	45	375	5.64e-42	149
Msa0854730	MtrunA17_Chr7g0219171	54.478	134	58	3	196	328	117	248	6.79e-42	145
Msa0854730	MtrunA17_Chr7g0219171	46.667	90	44	2	1	90	31	116	1.05e-14	72.8
Msa0854730	MtrunA17_Chr8g0367501	30.841	321	210	7	1	313	32	348	1.34e-41	149
Msa0854730	MtrunA17_Chr7g0218261	29.874	318	213	7	3	314	40	353	3.80e-41	147
Msa0854730	MtrunA17_Chr8g0345851	33.813	278	168	8	55	327	23	289	5.21e-41	144
Msa0854730	MtrunA17_Chr2g0307521	35.185	216	130	4	101	313	139	347	1.50e-40	145
Msa0854730	MtrunA17_Chr1g0163601	31.928	332	193	10	3	315	38	355	3.59e-40	144
Msa0854730	MtrunA17_Chr1g0163611	38.500	200	119	3	116	315	160	355	1.60e-39	142
Msa0854730	MtrunA17_Chr7g0218301	33.636	220	142	3	95	314	145	360	2.24e-39	142
Msa0854730	MtrunA17_Chr5g0448091	31.098	328	203	10	1	315	33	350	4.36e-39	141
Msa0854730	MtrunA17_Chr7g0218271	32.127	221	146	3	95	315	145	361	4.75e-39	141
Msa0854730	MtrunA17_Chr7g0218131	28.438	320	218	7	3	315	41	356	9.70e-39	140
Msa0854730	MtrunA17_Chr7g0218181	32.727	220	144	3	95	314	137	352	1.33e-38	140
Msa0854730	MtrunA17_Chr1g0163621	38.500	200	119	3	116	315	174	369	3.49e-38	139
Msa0854730	MtrunA17_Chr3g0085211	29.781	319	213	6	3	315	43	356	1.90e-37	137
Msa0854730	MtrunA17_Chr7g0218211	28.349	321	215	8	3	314	40	354	5.78e-37	136
Msa0854730	MtrunA17_Chr3g0085201	29.907	321	208	9	3	314	30	342	6.12e-37	135
Msa0854730	MtrunA17_Chr8g0367381	39.779	181	104	4	116	295	149	325	2.83e-35	130
Msa0854730	MtrunA17_Chr2g0314271	29.688	320	211	9	1	313	27	339	7.16e-31	119
Msa0854730	MtrunA17_Chr3g0130061	26.959	319	220	9	2	313	31	343	3.86e-27	109
Msa0854730	MtrunA17_Chr3g0130011	27.964	329	204	13	2	313	31	343	1.04e-26	108
Msa0854730	MtrunA17_Chr6g0482291	35.632	174	97	6	142	313	1	161	1.45e-22	92.8
Msa0854730	MtrunA17_Chr8g0367401	38.182	110	67	1	119	228	2	110	9.36e-21	87.0
Msa0854730	MtrunA17_Chr4g0014611	56.566	99	43	0	230	328	9	107	1.19e-19	82.8
Msa0854730	MtrunA17_Chr8g0367461	46.465	99	45	5	215	310	1	94	3.67e-19	81.6
Msa0854730	MtrunA17_Chr4g0014671	55.238	105	47	0	224	328	30	134	3.91e-17	76.6
Msa0854730	MtrunA17_Chr8g0367421	32.727	110	56	2	119	228	2	93	1.45e-14	69.3
Msa0854730	MtrunA17_Chr4g0011691	42.708	96	51	4	230	324	140	232	6.96e-14	70.1
Msa0854730	MtrunA17_Chr8g0367391	39.604	101	57	3	228	327	36	133	1.62e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0854730	AT4G35160.1	35.484	341	200	9	2	327	45	380	6.23e-56	186
Msa0854730	AT4G35150.1	33.731	335	171	9	2	327	31	323	6.59e-52	174
Msa0854730	AT5G54160.1	32.188	320	205	8	1	314	34	347	8.05e-47	162
Msa0854730	AT1G51990.2	31.193	327	211	6	13	328	39	362	9.33e-44	154
Msa0854730	AT1G51990.1	31.193	327	211	6	13	328	39	362	1.24e-43	154
Msa0854730	AT1G77520.1	30.636	346	212	10	1	327	44	380	2.83e-40	145
Msa0854730	AT5G53810.1	29.942	344	218	8	1	327	40	377	5.24e-37	136
Msa0854730	AT1G63140.2	33.528	343	206	11	1	327	44	380	6.21e-37	136
Msa0854730	AT1G63140.4	33.528	343	206	11	1	327	64	400	1.03e-36	136
Msa0854730	AT1G33030.1	31.889	323	204	11	1	314	21	336	1.18e-36	135
Msa0854730	AT3G53140.1	30.982	326	204	10	1	313	27	344	1.39e-36	135
Msa0854730	AT1G77530.1	30.233	344	216	9	1	327	44	380	3.20e-33	126
Msa0854730	AT1G21130.1	28.182	330	211	9	1	314	40	359	5.99e-32	123
Msa0854730	AT1G62900.1	39.423	208	120	4	122	327	1	204	6.42e-31	116
Msa0854730	AT1G21110.1	27.607	326	210	9	1	310	40	355	2.52e-30	118
Msa0854730	AT1G21120.1	27.576	330	213	9	1	314	40	359	4.36e-30	118
Msa0854730	AT5G37170.2	34.171	199	126	4	131	327	38	233	5.27e-30	114
Msa0854730	AT1G21120.2	27.576	330	213	9	1	314	88	407	1.17e-29	117
Msa0854730	AT1G21100.1	27.576	330	213	9	1	314	40	359	1.42e-29	116
Msa0854730	AT5G37170.1	34.171	199	126	4	131	327	138	333	1.63e-29	115
Msa0854730	AT5G37170.3	34.043	188	119	4	142	327	1	185	6.38e-27	105
Msa0854730	AT1G76790.1	24.695	328	217	7	1	309	30	346	5.48e-26	106
Msa0854730	AT1G33030.2	29.787	235	155	7	1	228	21	252	4.26e-18	82.8
Msa0854730	AT1G33030.3	29.787	235	155	7	1	228	21	252	6.09e-18	82.8
Msa0854730	AT1G63140.1	29.752	242	153	8	1	228	64	302	3.23e-17	81.3

Find 95 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGGTGGTCACACAATATT+TGG	0.168914	5_4:+47521387	Msa0854730:three_prime_UTR
GTTAAATGAGTTGTTAAATT+TGG	0.170908	5_4:-47520379	None:intergenic
ATAATTAATTGAATGTATTT+TGG	0.181853	5_4:+47521474	Msa0854730:three_prime_UTR
CAACACATGGGATGTCGTTT+TGG	0.213169	5_4:+47520337	Msa0854730:CDS
ATAGACCTTGATCCTAAAAT+TGG	0.254900	5_4:-47521163	None:intergenic
TTTGTCTTAAGAGGATGATT+TGG	0.260141	5_4:+47521368	Msa0854730:three_prime_UTR
CCATGGTTGTGTATGATATC+TGG	0.301298	5_4:-47520018	None:intergenic
GATCTCCCACATGTTGTTGA+TGG	0.310559	5_4:+47520564	Msa0854730:CDS
ATTATATAACACTTGCTAAC+TGG	0.321090	5_4:-47519823	None:intergenic
TGCCATGGATCTATCATATC+TGG	0.330412	5_4:-47520267	None:intergenic
ATGCGAATCATGACTTATTC+CGG	0.334688	5_4:+47520117	Msa0854730:CDS
TACAGTCGATATTGCATGAT+TGG	0.335498	5_4:+47520897	Msa0854730:intron
TTGTCTTAAGAGGATGATTT+GGG	0.336204	5_4:+47521369	Msa0854730:three_prime_UTR
TTTGGACATACGCACTTAAT+CGG	0.352771	5_4:+47521405	Msa0854730:three_prime_UTR
AGACAAGGAAAATACTTATA+TGG	0.355177	5_4:-47521539	None:intergenic
TCAAAGCTCAAAGCCACTTA+TGG	0.367540	5_4:+47519935	Msa0854730:five_prime_UTR
TCGATCAACAAGATTTGAAC+TGG	0.372789	5_4:-47519860	None:intergenic
CAACCAATGACTCAACTTCA+TGG	0.372843	5_4:-47520467	None:intergenic
ACAATGCAGAGGGATGGTTT+TGG	0.380712	5_4:-47521337	None:intergenic
TCGATTAGTTTCCGATTTGT+TGG	0.382710	5_4:+47520422	Msa0854730:CDS
TGCCAAATCATTCCCACAAA+TGG	0.391775	5_4:+47520527	Msa0854730:CDS
TGAAAATGTGATTCCATAAG+TGG	0.393208	5_4:-47519948	None:intergenic
TTCCATTTGTGGGAATGATT+TGG	0.394430	5_4:-47520529	None:intergenic
GCTTTGAGCTTTGAGCAAAT+TGG	0.396268	5_4:-47519926	None:intergenic
GTTGATGTTGGAGGAGGTAC+GGG	0.411565	5_4:+47520486	Msa0854730:CDS
AAGAGAGAAACGAGAAAGAA+TGG	0.424421	5_4:+47521095	Msa0854730:CDS
AACAAAATTGCATCTGTTGG+CGG	0.431252	5_4:-47520642	None:intergenic
AACACAATGCATTCCATTTG+TGG	0.431379	5_4:-47520540	None:intergenic
ATGGATTAAGTTGATTTCCT+TGG	0.431482	5_4:+47521114	Msa0854730:CDS
GTTCCATGAAGTTGAGTCAT+TGG	0.432542	5_4:+47520464	Msa0854730:CDS
CAACTCATTTAACGAATCAA+TGG	0.437712	5_4:+47520389	Msa0854730:CDS
AGCAACATTATCTCCATGTT+GGG	0.440877	5_4:-47519902	None:intergenic
CTTAAATGTGTAGTTGACTT+AGG	0.449531	5_4:+47519991	Msa0854730:CDS
GCAATATCAAAGAAAGGTAA+AGG	0.460727	5_4:+47520962	Msa0854730:CDS
CTTATATGGTCACAATAGCT+TGG	0.463243	5_4:-47521525	None:intergenic
GCACTTCTCAACCAACAAAT+CGG	0.467046	5_4:-47520433	None:intergenic
CATGGCATCAATTCTCTGCT+TGG	0.468036	5_4:+47520283	Msa0854730:CDS
GTTGAGTCATTGGTTGATGT+TGG	0.468409	5_4:+47520474	Msa0854730:CDS
ATTAAGTTGATTTCCTTGGC+TGG	0.468848	5_4:+47521118	Msa0854730:CDS
GGTTGATGTTGGAGGAGGTA+CGG	0.474379	5_4:+47520485	Msa0854730:CDS
TTCCTTCTTCCTCATTGTAG+AGG	0.479935	5_4:+47521290	Msa0854730:three_prime_UTR
TTCAACAAAATTGCATCTGT+TGG	0.490404	5_4:-47520645	None:intergenic
GCACTTAATCGGAAAATAAG+TGG	0.499566	5_4:+47521416	Msa0854730:three_prime_UTR
ACACAATGCATTCCATTTGT+GGG	0.501519	5_4:-47520539	None:intergenic
AAAGATATTAAAGAATTGTA+AGG	0.505831	5_4:+47520937	Msa0854730:CDS
TTATATGGTCACAATAGCTT+GGG	0.511873	5_4:-47521524	None:intergenic
AAGCAACATTATCTCCATGT+TGG	0.524077	5_4:-47519903	None:intergenic
ACATGTGGTGCCACACTCAT+TGG	0.525895	5_4:-47520228	None:intergenic
AACATTGTGATGATGACAAA+TGG	0.526932	5_4:-47521226	None:intergenic
AGTGAAAACTTAAACTATGT+TGG	0.531848	5_4:+47520597	Msa0854730:CDS
CAATATCAAAGAAAGGTAAA+GGG	0.532814	5_4:+47520963	Msa0854730:CDS
CTCCTCTACAATGAGGAAGA+AGG	0.539728	5_4:-47521292	None:intergenic
TTGATGATGGTAGTATTCGC+AGG	0.543043	5_4:+47521070	Msa0854730:CDS
CGATCAACAAGATTTGAACT+GGG	0.544734	5_4:-47519859	None:intergenic
AAGGATGCAATATCAAAGAA+AGG	0.545671	5_4:+47520956	Msa0854730:CDS
TCATCCTCTTAAGACAAAAG+AGG	0.545757	5_4:-47521363	None:intergenic
GAAAACTTAAACTATGTTGG+TGG	0.548643	5_4:+47520600	Msa0854730:CDS
CATCCTCTTAAGACAAAAGA+GGG	0.549685	5_4:-47521362	None:intergenic
CTTCTATGACATGTTGATGA+TGG	0.553245	5_4:+47521057	Msa0854730:CDS
GGAATAGAGATGTAGAGAAA+CGG	0.554790	5_4:-47521271	None:intergenic
CATGTTGTTGATGGTTTGCA+AGG	0.557883	5_4:+47520573	Msa0854730:CDS
TCATTGTAGAGGAGTGATTG+TGG	0.562080	5_4:+47521301	Msa0854730:three_prime_UTR
CGCATCAAGCGATAGATGCA+AGG	0.562811	5_4:-47520099	None:intergenic
ACGGTGTTAGACAAAGAGAA+TGG	0.563271	5_4:+47521004	Msa0854730:CDS
GAGTCATTGGTTGATGTTGG+AGG	0.570761	5_4:+47520477	Msa0854730:CDS
TGCAAACCATCAACAACATG+TGG	0.573332	5_4:-47520570	None:intergenic
AAAGAAAGGTAAAGGGAAGG+TGG	0.580286	5_4:+47520970	Msa0854730:CDS
TGGAGGAGGTACGGGGACCA+TGG	0.588947	5_4:+47520494	Msa0854730:CDS
CAAGCAGAGAATTGATGCCA+TGG	0.591666	5_4:-47520282	None:intergenic
CTGAGTATCCCGTGCACATG+TGG	0.594919	5_4:-47520243	None:intergenic
GTAGTCACTAAAACCAGCCA+AGG	0.595683	5_4:-47521131	None:intergenic
TTGTTGGTTGAGAAGTGCAA+GGG	0.598365	5_4:+47520438	Msa0854730:CDS
TTTGTTGGTTGAGAAGTGCA+AGG	0.604264	5_4:+47520437	Msa0854730:CDS
GTCCAGATATGATAGATCCA+TGG	0.611089	5_4:+47520265	Msa0854730:CDS
ATCAAAGAAAGGTAAAGGGA+AGG	0.618639	5_4:+47520967	Msa0854730:CDS
TCATTGGTTGATGTTGGAGG+AGG	0.622074	5_4:+47520480	Msa0854730:CDS
TCTTAAGAGGATGATTTGGG+TGG	0.624562	5_4:+47521372	Msa0854730:three_prime_UTR
AGGGAGACAATGCAGAGGGA+TGG	0.628535	5_4:-47521343	None:intergenic
ATCAACAAGATTTGAACTGG+GGG	0.636051	5_4:-47519857	None:intergenic
TTGATGTTGGAGGAGGTACG+GGG	0.638274	5_4:+47520487	Msa0854730:CDS
GAAAATCACTTATTTAGACA+AGG	0.642671	5_4:-47521554	None:intergenic
GTGTGGCACCACATGTGCAC+GGG	0.643351	5_4:+47520235	Msa0854730:CDS
CCAGATATCATACACAACCA+TGG	0.643900	5_4:+47520018	Msa0854730:CDS
ACAGCATTTGAAACAACACA+TGG	0.644519	5_4:+47520324	Msa0854730:CDS
AGTGTGGCACCACATGTGCA+CGG	0.654606	5_4:+47520234	Msa0854730:CDS
AAAGAGGGAGACAATGCAGA+GGG	0.658598	5_4:-47521347	None:intergenic
GATCAACAAGATTTGAACTG+GGG	0.663002	5_4:-47519858	None:intergenic
AAAAGAGGGAGACAATGCAG+AGG	0.667854	5_4:-47521348	None:intergenic
TAAGAACAGTCCAATGAGTG+TGG	0.672166	5_4:+47520218	Msa0854730:CDS
ACAATCACTCCTCTACAATG+AGG	0.686669	5_4:-47521299	None:intergenic
GCAAACCATCAACAACATGT+GGG	0.690290	5_4:-47520569	None:intergenic
GTAGCTAGCGAATCCCAACA+TGG	0.697518	5_4:+47519889	Msa0854730:five_prime_UTR
GAAGGTGGTTATCATTGACA+CGG	0.700974	5_4:+47520985	Msa0854730:CDS
ATTGCATGATTGGAATGACG+AGG	0.708926	5_4:+47520907	Msa0854730:CDS
CAGCATTTGAAACAACACAT+GGG	0.716263	5_4:+47520325	Msa0854730:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTAATTTGATTTTTTTTTCT+TGG	+	chr5_4:47520719-47520738	Msa0854730:intron	10.0%
!!!	ATAATTAATTGAATGTATTT+TGG	+	chr5_4:47521474-47521493	Msa0854730:three_prime_UTR	10.0%
!!!	TTAATTTGATTTTTTTTTCT+TGG	+	chr5_4:47520719-47520738	Msa0854730:intron	10.0%
!!!	ATAATTAATTGAATGTATTT+TGG	+	chr5_4:47521474-47521493	Msa0854730:three_prime_UTR	10.0%
!!!	TATATTAGTTTACACATTAT+AGG	+	chr5_4:47520695-47520714	Msa0854730:intron	15.0%
!!!	ATATTAGTTTACACATTATA+GGG	+	chr5_4:47520696-47520715	Msa0854730:intron	15.0%
!!	AAGGAAAAATATTATCTAAT+TGG	-	chr5_4:47520799-47520818	None:intergenic	15.0%
!!	AAAGATATTAAAGAATTGTA+AGG	+	chr5_4:47520937-47520956	Msa0854730:CDS	15.0%
!!!	TATATTAGTTTACACATTAT+AGG	+	chr5_4:47520695-47520714	Msa0854730:intron	15.0%
!!!	ATATTAGTTTACACATTATA+GGG	+	chr5_4:47520696-47520715	Msa0854730:intron	15.0%
!!	AAGGAAAAATATTATCTAAT+TGG	-	chr5_4:47520799-47520818	None:intergenic	15.0%
!!	AAAGATATTAAAGAATTGTA+AGG	+	chr5_4:47520937-47520956	Msa0854730:CDS	15.0%
!!	GTTAAATGAGTTGTTAAATT+TGG	-	chr5_4:47520382-47520401	None:intergenic	20.0%
!!!	ATTTTATAGATTATGGATCA+TGG	-	chr5_4:47521202-47521221	None:intergenic	20.0%
!!	GTTAAATGAGTTGTTAAATT+TGG	-	chr5_4:47520382-47520401	None:intergenic	20.0%
!!!	ATTTTATAGATTATGGATCA+TGG	-	chr5_4:47521202-47521221	None:intergenic	20.0%
!!	ATTATATAACACTTGCTAAC+TGG	-	chr5_4:47519826-47519845	None:intergenic	25.0%
!	AGTGAAAACTTAAACTATGT+TGG	+	chr5_4:47520597-47520616	Msa0854730:CDS	25.0%
!!!	TTTTTTTCTTGGTACAAAAG+AGG	+	chr5_4:47520730-47520749	Msa0854730:intron	25.0%
!!!	TTTTTTCTTGGTACAAAAGA+GGG	+	chr5_4:47520731-47520750	Msa0854730:intron	25.0%
!!	CCATATTTTTCTACTAATGA+CGG	+	chr5_4:47520773-47520792	Msa0854730:intron	25.0%
!	AGTGGAAAAAATAGAAAACA+AGG	-	chr5_4:47520818-47520837	None:intergenic	25.0%
!	CAATATCAAAGAAAGGTAAA+GGG	+	chr5_4:47520963-47520982	Msa0854730:CDS	25.0%
!!	TACAAGATTACTCCAATTTT+AGG	+	chr5_4:47521151-47521170	Msa0854730:CDS	25.0%
!!	TGGCTAGATTTTATAGATTA+TGG	-	chr5_4:47521209-47521228	None:intergenic	25.0%
!	AGACAAGGAAAATACTTATA+TGG	-	chr5_4:47521542-47521561	None:intergenic	25.0%
!	GAAAATCACTTATTTAGACA+AGG	-	chr5_4:47521557-47521576	None:intergenic	25.0%
!!	ATTATATAACACTTGCTAAC+TGG	-	chr5_4:47519826-47519845	None:intergenic	25.0%
!	AGTGAAAACTTAAACTATGT+TGG	+	chr5_4:47520597-47520616	Msa0854730:CDS	25.0%
!!!	TTTTTTTCTTGGTACAAAAG+AGG	+	chr5_4:47520730-47520749	Msa0854730:intron	25.0%
!!!	TTTTTTCTTGGTACAAAAGA+GGG	+	chr5_4:47520731-47520750	Msa0854730:intron	25.0%
!!	CCATATTTTTCTACTAATGA+CGG	+	chr5_4:47520773-47520792	Msa0854730:intron	25.0%
!	AGTGGAAAAAATAGAAAACA+AGG	-	chr5_4:47520818-47520837	None:intergenic	25.0%
!	CAATATCAAAGAAAGGTAAA+GGG	+	chr5_4:47520963-47520982	Msa0854730:CDS	25.0%
!!	TACAAGATTACTCCAATTTT+AGG	+	chr5_4:47521151-47521170	Msa0854730:CDS	25.0%
!!	TGGCTAGATTTTATAGATTA+TGG	-	chr5_4:47521209-47521228	None:intergenic	25.0%
!	AGACAAGGAAAATACTTATA+TGG	-	chr5_4:47521542-47521561	None:intergenic	25.0%
!	GAAAATCACTTATTTAGACA+AGG	-	chr5_4:47521557-47521576	None:intergenic	25.0%
	TGAAAATGTGATTCCATAAG+TGG	-	chr5_4:47519951-47519970	None:intergenic	30.0%
!	CTTAAATGTGTAGTTGACTT+AGG	+	chr5_4:47519991-47520010	Msa0854730:CDS	30.0%
	CAACTCATTTAACGAATCAA+TGG	+	chr5_4:47520389-47520408	Msa0854730:CDS	30.0%
	GAAAACTTAAACTATGTTGG+TGG	+	chr5_4:47520600-47520619	Msa0854730:CDS	30.0%
	TTCAACAAAATTGCATCTGT+TGG	-	chr5_4:47520648-47520667	None:intergenic	30.0%
!	AACAGATGCAATTTTGTTGA+AGG	+	chr5_4:47520647-47520666	Msa0854730:CDS	30.0%
!	TTTTTCTTGGTACAAAAGAG+GGG	+	chr5_4:47520732-47520751	Msa0854730:intron	30.0%
	CCGTCATTAGTAGAAAAATA+TGG	-	chr5_4:47520776-47520795	None:intergenic	30.0%
	GAAAAGAGAACAAACTAAAG+TGG	-	chr5_4:47520836-47520855	None:intergenic	30.0%
!	TATGTTTGAGTTACGAAAGA+AGG	-	chr5_4:47520864-47520883	None:intergenic	30.0%
	AAGGATGCAATATCAAAGAA+AGG	+	chr5_4:47520956-47520975	Msa0854730:CDS	30.0%
	GCAATATCAAAGAAAGGTAA+AGG	+	chr5_4:47520962-47520981	Msa0854730:CDS	30.0%
!!	ATGGATTAAGTTGATTTCCT+TGG	+	chr5_4:47521114-47521133	Msa0854730:CDS	30.0%
!!!	TTACTCCAATTTTAGGATCA+AGG	+	chr5_4:47521158-47521177	Msa0854730:CDS	30.0%
	ATAGACCTTGATCCTAAAAT+TGG	-	chr5_4:47521166-47521185	None:intergenic	30.0%
!	AACATTGTGATGATGACAAA+TGG	-	chr5_4:47521229-47521248	None:intergenic	30.0%
	TTTGTCTTAAGAGGATGATT+TGG	+	chr5_4:47521368-47521387	Msa0854730:three_prime_UTR	30.0%
	TTGTCTTAAGAGGATGATTT+GGG	+	chr5_4:47521369-47521388	Msa0854730:three_prime_UTR	30.0%
	TTATATGGTCACAATAGCTT+GGG	-	chr5_4:47521527-47521546	None:intergenic	30.0%
	TGAAAATGTGATTCCATAAG+TGG	-	chr5_4:47519951-47519970	None:intergenic	30.0%
!	CTTAAATGTGTAGTTGACTT+AGG	+	chr5_4:47519991-47520010	Msa0854730:CDS	30.0%
	CAACTCATTTAACGAATCAA+TGG	+	chr5_4:47520389-47520408	Msa0854730:CDS	30.0%
	GAAAACTTAAACTATGTTGG+TGG	+	chr5_4:47520600-47520619	Msa0854730:CDS	30.0%
	TTCAACAAAATTGCATCTGT+TGG	-	chr5_4:47520648-47520667	None:intergenic	30.0%
!	AACAGATGCAATTTTGTTGA+AGG	+	chr5_4:47520647-47520666	Msa0854730:CDS	30.0%
!	TTTTTCTTGGTACAAAAGAG+GGG	+	chr5_4:47520732-47520751	Msa0854730:intron	30.0%
	CCGTCATTAGTAGAAAAATA+TGG	-	chr5_4:47520776-47520795	None:intergenic	30.0%
	GAAAAGAGAACAAACTAAAG+TGG	-	chr5_4:47520836-47520855	None:intergenic	30.0%
!	TATGTTTGAGTTACGAAAGA+AGG	-	chr5_4:47520864-47520883	None:intergenic	30.0%
	AAGGATGCAATATCAAAGAA+AGG	+	chr5_4:47520956-47520975	Msa0854730:CDS	30.0%
	GCAATATCAAAGAAAGGTAA+AGG	+	chr5_4:47520962-47520981	Msa0854730:CDS	30.0%
!!	ATGGATTAAGTTGATTTCCT+TGG	+	chr5_4:47521114-47521133	Msa0854730:CDS	30.0%
!!!	TTACTCCAATTTTAGGATCA+AGG	+	chr5_4:47521158-47521177	Msa0854730:CDS	30.0%
	ATAGACCTTGATCCTAAAAT+TGG	-	chr5_4:47521166-47521185	None:intergenic	30.0%
!	AACATTGTGATGATGACAAA+TGG	-	chr5_4:47521229-47521248	None:intergenic	30.0%
	TTTGTCTTAAGAGGATGATT+TGG	+	chr5_4:47521368-47521387	Msa0854730:three_prime_UTR	30.0%
	TTGTCTTAAGAGGATGATTT+GGG	+	chr5_4:47521369-47521388	Msa0854730:three_prime_UTR	30.0%
	TTATATGGTCACAATAGCTT+GGG	-	chr5_4:47521527-47521546	None:intergenic	30.0%
	ATCAACAAGATTTGAACTGG+GGG	-	chr5_4:47519860-47519879	None:intergenic	35.0%
	GATCAACAAGATTTGAACTG+GGG	-	chr5_4:47519861-47519880	None:intergenic	35.0%
	CGATCAACAAGATTTGAACT+GGG	-	chr5_4:47519862-47519881	None:intergenic	35.0%
	TCGATCAACAAGATTTGAAC+TGG	-	chr5_4:47519863-47519882	None:intergenic	35.0%
	AGCAACATTATCTCCATGTT+GGG	-	chr5_4:47519905-47519924	None:intergenic	35.0%
	AAGCAACATTATCTCCATGT+TGG	-	chr5_4:47519906-47519925	None:intergenic	35.0%
!!!	GAAGAAATGAGTTTTGAGAG+TGG	-	chr5_4:47520054-47520073	None:intergenic	35.0%
!!!	AGATGCAAGGCTTTTTTGAA+GGG	-	chr5_4:47520089-47520108	None:intergenic	35.0%
!!!	TAGATGCAAGGCTTTTTTGA+AGG	-	chr5_4:47520090-47520109	None:intergenic	35.0%
	ATGCGAATCATGACTTATTC+CGG	+	chr5_4:47520117-47520136	Msa0854730:CDS	35.0%
!	ATTTTGTTGAGAGAAGAAGC+CGG	-	chr5_4:47520139-47520158	None:intergenic	35.0%
!	ACAGCATTTGAAACAACACA+TGG	+	chr5_4:47520324-47520343	Msa0854730:CDS	35.0%
!	CAGCATTTGAAACAACACAT+GGG	+	chr5_4:47520325-47520344	Msa0854730:CDS	35.0%
!!	TCGATTAGTTTCCGATTTGT+TGG	+	chr5_4:47520422-47520441	Msa0854730:CDS	35.0%
	TTCCATTTGTGGGAATGATT+TGG	-	chr5_4:47520532-47520551	None:intergenic	35.0%
	ACACAATGCATTCCATTTGT+GGG	-	chr5_4:47520542-47520561	None:intergenic	35.0%
	AACACAATGCATTCCATTTG+TGG	-	chr5_4:47520543-47520562	None:intergenic	35.0%
	AACAAAATTGCATCTGTTGG+CGG	-	chr5_4:47520645-47520664	None:intergenic	35.0%
	TACAGTCGATATTGCATGAT+TGG	+	chr5_4:47520897-47520916	Msa0854730:intron	35.0%
	ATCAAAGAAAGGTAAAGGGA+AGG	+	chr5_4:47520967-47520986	Msa0854730:CDS	35.0%
!	CTTCTATGACATGTTGATGA+TGG	+	chr5_4:47521057-47521076	Msa0854730:CDS	35.0%
	AAGAGAGAAACGAGAAAGAA+TGG	+	chr5_4:47521095-47521114	Msa0854730:CDS	35.0%
!!	ATTAAGTTGATTTCCTTGGC+TGG	+	chr5_4:47521118-47521137	Msa0854730:CDS	35.0%
	GGAATAGAGATGTAGAGAAA+CGG	-	chr5_4:47521274-47521293	None:intergenic	35.0%
	CATCCTCTTAAGACAAAAGA+GGG	-	chr5_4:47521365-47521384	None:intergenic	35.0%
	TCATCCTCTTAAGACAAAAG+AGG	-	chr5_4:47521366-47521385	None:intergenic	35.0%
	TTTGGACATACGCACTTAAT+CGG	+	chr5_4:47521405-47521424	Msa0854730:three_prime_UTR	35.0%
	GCACTTAATCGGAAAATAAG+TGG	+	chr5_4:47521416-47521435	Msa0854730:three_prime_UTR	35.0%
	CTTATATGGTCACAATAGCT+TGG	-	chr5_4:47521528-47521547	None:intergenic	35.0%
	ATCAACAAGATTTGAACTGG+GGG	-	chr5_4:47519860-47519879	None:intergenic	35.0%
	GATCAACAAGATTTGAACTG+GGG	-	chr5_4:47519861-47519880	None:intergenic	35.0%
	CGATCAACAAGATTTGAACT+GGG	-	chr5_4:47519862-47519881	None:intergenic	35.0%
	TCGATCAACAAGATTTGAAC+TGG	-	chr5_4:47519863-47519882	None:intergenic	35.0%
	AGCAACATTATCTCCATGTT+GGG	-	chr5_4:47519905-47519924	None:intergenic	35.0%
	AAGCAACATTATCTCCATGT+TGG	-	chr5_4:47519906-47519925	None:intergenic	35.0%
!!!	GAAGAAATGAGTTTTGAGAG+TGG	-	chr5_4:47520054-47520073	None:intergenic	35.0%
!!!	AGATGCAAGGCTTTTTTGAA+GGG	-	chr5_4:47520089-47520108	None:intergenic	35.0%
!!!	TAGATGCAAGGCTTTTTTGA+AGG	-	chr5_4:47520090-47520109	None:intergenic	35.0%
	ATGCGAATCATGACTTATTC+CGG	+	chr5_4:47520117-47520136	Msa0854730:CDS	35.0%
!	ATTTTGTTGAGAGAAGAAGC+CGG	-	chr5_4:47520139-47520158	None:intergenic	35.0%
!	ACAGCATTTGAAACAACACA+TGG	+	chr5_4:47520324-47520343	Msa0854730:CDS	35.0%
!	CAGCATTTGAAACAACACAT+GGG	+	chr5_4:47520325-47520344	Msa0854730:CDS	35.0%
!!	TCGATTAGTTTCCGATTTGT+TGG	+	chr5_4:47520422-47520441	Msa0854730:CDS	35.0%
	TTCCATTTGTGGGAATGATT+TGG	-	chr5_4:47520532-47520551	None:intergenic	35.0%
	ACACAATGCATTCCATTTGT+GGG	-	chr5_4:47520542-47520561	None:intergenic	35.0%
	AACACAATGCATTCCATTTG+TGG	-	chr5_4:47520543-47520562	None:intergenic	35.0%
	AACAAAATTGCATCTGTTGG+CGG	-	chr5_4:47520645-47520664	None:intergenic	35.0%
	TACAGTCGATATTGCATGAT+TGG	+	chr5_4:47520897-47520916	Msa0854730:intron	35.0%
	ATCAAAGAAAGGTAAAGGGA+AGG	+	chr5_4:47520967-47520986	Msa0854730:CDS	35.0%
!	CTTCTATGACATGTTGATGA+TGG	+	chr5_4:47521057-47521076	Msa0854730:CDS	35.0%
	AAGAGAGAAACGAGAAAGAA+TGG	+	chr5_4:47521095-47521114	Msa0854730:CDS	35.0%
!!	ATTAAGTTGATTTCCTTGGC+TGG	+	chr5_4:47521118-47521137	Msa0854730:CDS	35.0%
	GGAATAGAGATGTAGAGAAA+CGG	-	chr5_4:47521274-47521293	None:intergenic	35.0%
	CATCCTCTTAAGACAAAAGA+GGG	-	chr5_4:47521365-47521384	None:intergenic	35.0%
	TCATCCTCTTAAGACAAAAG+AGG	-	chr5_4:47521366-47521385	None:intergenic	35.0%
	TTTGGACATACGCACTTAAT+CGG	+	chr5_4:47521405-47521424	Msa0854730:three_prime_UTR	35.0%
	GCACTTAATCGGAAAATAAG+TGG	+	chr5_4:47521416-47521435	Msa0854730:three_prime_UTR	35.0%
	CTTATATGGTCACAATAGCT+TGG	-	chr5_4:47521528-47521547	None:intergenic	35.0%
!!	GCTTTGAGCTTTGAGCAAAT+TGG	-	chr5_4:47519929-47519948	None:intergenic	40.0%
	TCAAAGCTCAAAGCCACTTA+TGG	+	chr5_4:47519935-47519954	Msa0854730:five_prime_UTR	40.0%
	CCATGGTTGTGTATGATATC+TGG	-	chr5_4:47520021-47520040	None:intergenic	40.0%
	CCAGATATCATACACAACCA+TGG	+	chr5_4:47520018-47520037	Msa0854730:CDS	40.0%
!!!	ATGAGTTTTGAGAGTGGCAT+GGG	-	chr5_4:47520048-47520067	None:intergenic	40.0%
!!!	AATGAGTTTTGAGAGTGGCA+TGG	-	chr5_4:47520049-47520068	None:intergenic	40.0%
	TAAGAACAGTCCAATGAGTG+TGG	+	chr5_4:47520218-47520237	Msa0854730:CDS	40.0%
	GTCCAGATATGATAGATCCA+TGG	+	chr5_4:47520265-47520284	Msa0854730:CDS	40.0%
	TGCCATGGATCTATCATATC+TGG	-	chr5_4:47520270-47520289	None:intergenic	40.0%
!!!	AACACATGGGATGTCGTTTT+GGG	+	chr5_4:47520338-47520357	Msa0854730:CDS	40.0%
	GCACTTCTCAACCAACAAAT+CGG	-	chr5_4:47520436-47520455	None:intergenic	40.0%
!	TTTGTTGGTTGAGAAGTGCA+AGG	+	chr5_4:47520437-47520456	Msa0854730:CDS	40.0%
!	TTGTTGGTTGAGAAGTGCAA+GGG	+	chr5_4:47520438-47520457	Msa0854730:CDS	40.0%
	GTTCCATGAAGTTGAGTCAT+TGG	+	chr5_4:47520464-47520483	Msa0854730:CDS	40.0%
	CAACCAATGACTCAACTTCA+TGG	-	chr5_4:47520470-47520489	None:intergenic	40.0%
!	GTTGAGTCATTGGTTGATGT+TGG	+	chr5_4:47520474-47520493	Msa0854730:CDS	40.0%
!	TTTGGCAAGAGTTTTTGCCA+TGG	-	chr5_4:47520514-47520533	None:intergenic	40.0%
	TGCCAAATCATTCCCACAAA+TGG	+	chr5_4:47520527-47520546	Msa0854730:CDS	40.0%
	GCAAACCATCAACAACATGT+GGG	-	chr5_4:47520572-47520591	None:intergenic	40.0%
	TGCAAACCATCAACAACATG+TGG	-	chr5_4:47520573-47520592	None:intergenic	40.0%
!!	CATGTTGTTGATGGTTTGCA+AGG	+	chr5_4:47520573-47520592	Msa0854730:CDS	40.0%
	ATTGCATGATTGGAATGACG+AGG	+	chr5_4:47520907-47520926	Msa0854730:CDS	40.0%
	AAAGAAAGGTAAAGGGAAGG+TGG	+	chr5_4:47520970-47520989	Msa0854730:CDS	40.0%
	GAAGGTGGTTATCATTGACA+CGG	+	chr5_4:47520985-47521004	Msa0854730:CDS	40.0%
!	ACGGTGTTAGACAAAGAGAA+TGG	+	chr5_4:47521004-47521023	Msa0854730:CDS	40.0%
!!	TTGATGATGGTAGTATTCGC+AGG	+	chr5_4:47521070-47521089	Msa0854730:CDS	40.0%
	TTCCTTCTTCCTCATTGTAG+AGG	+	chr5_4:47521290-47521309	Msa0854730:three_prime_UTR	40.0%
	ACAATCACTCCTCTACAATG+AGG	-	chr5_4:47521302-47521321	None:intergenic	40.0%
	TCATTGTAGAGGAGTGATTG+TGG	+	chr5_4:47521301-47521320	Msa0854730:three_prime_UTR	40.0%
!	TCTCCCTCTTTTGTCTTAAG+AGG	+	chr5_4:47521359-47521378	Msa0854730:three_prime_UTR	40.0%
!	TCTTAAGAGGATGATTTGGG+TGG	+	chr5_4:47521372-47521391	Msa0854730:three_prime_UTR	40.0%
	TTGGGTGGTCACACAATATT+TGG	+	chr5_4:47521387-47521406	Msa0854730:three_prime_UTR	40.0%
!!	GCTTTGAGCTTTGAGCAAAT+TGG	-	chr5_4:47519929-47519948	None:intergenic	40.0%
	TCAAAGCTCAAAGCCACTTA+TGG	+	chr5_4:47519935-47519954	Msa0854730:five_prime_UTR	40.0%
	CCATGGTTGTGTATGATATC+TGG	-	chr5_4:47520021-47520040	None:intergenic	40.0%
	CCAGATATCATACACAACCA+TGG	+	chr5_4:47520018-47520037	Msa0854730:CDS	40.0%
!!!	ATGAGTTTTGAGAGTGGCAT+GGG	-	chr5_4:47520048-47520067	None:intergenic	40.0%
!!!	AATGAGTTTTGAGAGTGGCA+TGG	-	chr5_4:47520049-47520068	None:intergenic	40.0%
	TAAGAACAGTCCAATGAGTG+TGG	+	chr5_4:47520218-47520237	Msa0854730:CDS	40.0%
	GTCCAGATATGATAGATCCA+TGG	+	chr5_4:47520265-47520284	Msa0854730:CDS	40.0%
	TGCCATGGATCTATCATATC+TGG	-	chr5_4:47520270-47520289	None:intergenic	40.0%
!!!	AACACATGGGATGTCGTTTT+GGG	+	chr5_4:47520338-47520357	Msa0854730:CDS	40.0%
	GCACTTCTCAACCAACAAAT+CGG	-	chr5_4:47520436-47520455	None:intergenic	40.0%
!	TTTGTTGGTTGAGAAGTGCA+AGG	+	chr5_4:47520437-47520456	Msa0854730:CDS	40.0%
!	TTGTTGGTTGAGAAGTGCAA+GGG	+	chr5_4:47520438-47520457	Msa0854730:CDS	40.0%
	GTTCCATGAAGTTGAGTCAT+TGG	+	chr5_4:47520464-47520483	Msa0854730:CDS	40.0%
	CAACCAATGACTCAACTTCA+TGG	-	chr5_4:47520470-47520489	None:intergenic	40.0%
!	GTTGAGTCATTGGTTGATGT+TGG	+	chr5_4:47520474-47520493	Msa0854730:CDS	40.0%
!	TTTGGCAAGAGTTTTTGCCA+TGG	-	chr5_4:47520514-47520533	None:intergenic	40.0%
	TGCCAAATCATTCCCACAAA+TGG	+	chr5_4:47520527-47520546	Msa0854730:CDS	40.0%
	GCAAACCATCAACAACATGT+GGG	-	chr5_4:47520572-47520591	None:intergenic	40.0%
	TGCAAACCATCAACAACATG+TGG	-	chr5_4:47520573-47520592	None:intergenic	40.0%
!!	CATGTTGTTGATGGTTTGCA+AGG	+	chr5_4:47520573-47520592	Msa0854730:CDS	40.0%
	ATTGCATGATTGGAATGACG+AGG	+	chr5_4:47520907-47520926	Msa0854730:CDS	40.0%
	AAAGAAAGGTAAAGGGAAGG+TGG	+	chr5_4:47520970-47520989	Msa0854730:CDS	40.0%
	GAAGGTGGTTATCATTGACA+CGG	+	chr5_4:47520985-47521004	Msa0854730:CDS	40.0%
!	ACGGTGTTAGACAAAGAGAA+TGG	+	chr5_4:47521004-47521023	Msa0854730:CDS	40.0%
!!	TTGATGATGGTAGTATTCGC+AGG	+	chr5_4:47521070-47521089	Msa0854730:CDS	40.0%
	TTCCTTCTTCCTCATTGTAG+AGG	+	chr5_4:47521290-47521309	Msa0854730:three_prime_UTR	40.0%
	ACAATCACTCCTCTACAATG+AGG	-	chr5_4:47521302-47521321	None:intergenic	40.0%
	TCATTGTAGAGGAGTGATTG+TGG	+	chr5_4:47521301-47521320	Msa0854730:three_prime_UTR	40.0%
!	TCTCCCTCTTTTGTCTTAAG+AGG	+	chr5_4:47521359-47521378	Msa0854730:three_prime_UTR	40.0%
!	TCTTAAGAGGATGATTTGGG+TGG	+	chr5_4:47521372-47521391	Msa0854730:three_prime_UTR	40.0%
	TTGGGTGGTCACACAATATT+TGG	+	chr5_4:47521387-47521406	Msa0854730:three_prime_UTR	40.0%
!!!	GGCTTTTTTGAAGGGTGGAT+TGG	-	chr5_4:47520081-47520100	None:intergenic	45.0%
!!!	TGCAAGGCTTTTTTGAAGGG+TGG	-	chr5_4:47520086-47520105	None:intergenic	45.0%
	CAAGCAGAGAATTGATGCCA+TGG	-	chr5_4:47520285-47520304	None:intergenic	45.0%
	CATGGCATCAATTCTCTGCT+TGG	+	chr5_4:47520283-47520302	Msa0854730:CDS	45.0%
!!	CAACACATGGGATGTCGTTT+TGG	+	chr5_4:47520337-47520356	Msa0854730:CDS	45.0%
!	GAGTCATTGGTTGATGTTGG+AGG	+	chr5_4:47520477-47520496	Msa0854730:CDS	45.0%
!	TCATTGGTTGATGTTGGAGG+AGG	+	chr5_4:47520480-47520499	Msa0854730:CDS	45.0%
	GATCTCCCACATGTTGTTGA+TGG	+	chr5_4:47520564-47520583	Msa0854730:CDS	45.0%
	GTAGTCACTAAAACCAGCCA+AGG	-	chr5_4:47521134-47521153	None:intergenic	45.0%
	CTCCTCTACAATGAGGAAGA+AGG	-	chr5_4:47521295-47521314	None:intergenic	45.0%
!	ACAATGCAGAGGGATGGTTT+TGG	-	chr5_4:47521340-47521359	None:intergenic	45.0%
	AAAGAGGGAGACAATGCAGA+GGG	-	chr5_4:47521350-47521369	None:intergenic	45.0%
	AAAAGAGGGAGACAATGCAG+AGG	-	chr5_4:47521351-47521370	None:intergenic	45.0%
!!!	GGCTTTTTTGAAGGGTGGAT+TGG	-	chr5_4:47520081-47520100	None:intergenic	45.0%
!!!	TGCAAGGCTTTTTTGAAGGG+TGG	-	chr5_4:47520086-47520105	None:intergenic	45.0%
	CAAGCAGAGAATTGATGCCA+TGG	-	chr5_4:47520285-47520304	None:intergenic	45.0%
	CATGGCATCAATTCTCTGCT+TGG	+	chr5_4:47520283-47520302	Msa0854730:CDS	45.0%
!!	CAACACATGGGATGTCGTTT+TGG	+	chr5_4:47520337-47520356	Msa0854730:CDS	45.0%
!	GAGTCATTGGTTGATGTTGG+AGG	+	chr5_4:47520477-47520496	Msa0854730:CDS	45.0%
!	TCATTGGTTGATGTTGGAGG+AGG	+	chr5_4:47520480-47520499	Msa0854730:CDS	45.0%
	GATCTCCCACATGTTGTTGA+TGG	+	chr5_4:47520564-47520583	Msa0854730:CDS	45.0%
	GTAGTCACTAAAACCAGCCA+AGG	-	chr5_4:47521134-47521153	None:intergenic	45.0%
	CTCCTCTACAATGAGGAAGA+AGG	-	chr5_4:47521295-47521314	None:intergenic	45.0%
!	ACAATGCAGAGGGATGGTTT+TGG	-	chr5_4:47521340-47521359	None:intergenic	45.0%
	AAAGAGGGAGACAATGCAGA+GGG	-	chr5_4:47521350-47521369	None:intergenic	45.0%
	AAAAGAGGGAGACAATGCAG+AGG	-	chr5_4:47521351-47521370	None:intergenic	45.0%
	GTAGCTAGCGAATCCCAACA+TGG	+	chr5_4:47519889-47519908	Msa0854730:five_prime_UTR	50.0%
!	AGAGTGGCATGGGTTTTCCA+TGG	-	chr5_4:47520038-47520057	None:intergenic	50.0%
	CGCATCAAGCGATAGATGCA+AGG	-	chr5_4:47520102-47520121	None:intergenic	50.0%
!!	ACATGTGGTGCCACACTCAT+TGG	-	chr5_4:47520231-47520250	None:intergenic	50.0%
!	GGTTGATGTTGGAGGAGGTA+CGG	+	chr5_4:47520485-47520504	Msa0854730:CDS	50.0%
!	GTTGATGTTGGAGGAGGTAC+GGG	+	chr5_4:47520486-47520505	Msa0854730:CDS	50.0%
!	TTGATGTTGGAGGAGGTACG+GGG	+	chr5_4:47520487-47520506	Msa0854730:CDS	50.0%
	GTAGCTAGCGAATCCCAACA+TGG	+	chr5_4:47519889-47519908	Msa0854730:five_prime_UTR	50.0%
!	AGAGTGGCATGGGTTTTCCA+TGG	-	chr5_4:47520038-47520057	None:intergenic	50.0%
	CGCATCAAGCGATAGATGCA+AGG	-	chr5_4:47520102-47520121	None:intergenic	50.0%
!!	ACATGTGGTGCCACACTCAT+TGG	-	chr5_4:47520231-47520250	None:intergenic	50.0%
!	GGTTGATGTTGGAGGAGGTA+CGG	+	chr5_4:47520485-47520504	Msa0854730:CDS	50.0%
!	GTTGATGTTGGAGGAGGTAC+GGG	+	chr5_4:47520486-47520505	Msa0854730:CDS	50.0%
!	TTGATGTTGGAGGAGGTACG+GGG	+	chr5_4:47520487-47520506	Msa0854730:CDS	50.0%
	AGTGTGGCACCACATGTGCA+CGG	+	chr5_4:47520234-47520253	Msa0854730:CDS	55.0%
	CTGAGTATCCCGTGCACATG+TGG	-	chr5_4:47520246-47520265	None:intergenic	55.0%
	AGGGAGACAATGCAGAGGGA+TGG	-	chr5_4:47521346-47521365	None:intergenic	55.0%
	AGTGTGGCACCACATGTGCA+CGG	+	chr5_4:47520234-47520253	Msa0854730:CDS	55.0%
	CTGAGTATCCCGTGCACATG+TGG	-	chr5_4:47520246-47520265	None:intergenic	55.0%
	AGGGAGACAATGCAGAGGGA+TGG	-	chr5_4:47521346-47521365	None:intergenic	55.0%
	GTGTGGCACCACATGTGCAC+GGG	+	chr5_4:47520235-47520254	Msa0854730:CDS	60.0%
	GTGTGGCACCACATGTGCAC+GGG	+	chr5_4:47520235-47520254	Msa0854730:CDS	60.0%
!	TGGAGGAGGTACGGGGACCA+TGG	+	chr5_4:47520494-47520513	Msa0854730:CDS	65.0%
!	TGGAGGAGGTACGGGGACCA+TGG	+	chr5_4:47520494-47520513	Msa0854730:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	47519786	47521656	47519786	ID=Msa0854730;Name=Msa0854730
chr5_4	mRNA	47519786	47521656	47519786	ID=Msa0854730-mRNA-1;Parent=Msa0854730;Name=Msa0854730-mRNA-1;_AED=0.01;_eAED=0.01;_QI=199\|1\|1\|1\|1\|1\|2\|452\|328
chr5_4	exon	47519786	47520668	47519786	ID=Msa0854730-mRNA-1:exon:14973;Parent=Msa0854730-mRNA-1
chr5_4	exon	47520902	47521656	47520902	ID=Msa0854730-mRNA-1:exon:14974;Parent=Msa0854730-mRNA-1
chr5_4	five_prime_UTR	47519786	47519984	47519786	ID=Msa0854730-mRNA-1:five_prime_utr;Parent=Msa0854730-mRNA-1
chr5_4	CDS	47519985	47520668	47519985	ID=Msa0854730-mRNA-1:cds;Parent=Msa0854730-mRNA-1
chr5_4	CDS	47520902	47521204	47520902	ID=Msa0854730-mRNA-1:cds;Parent=Msa0854730-mRNA-1
chr5_4	three_prime_UTR	47521205	47521656	47521205	ID=Msa0854730-mRNA-1:three_prime_utr;Parent=Msa0854730-mRNA-1

Gene Sequence

>Msa0854730

ATGTCACTTAAATGTGTAGTTGACTTAGGCATACCAGATATCATACACAACCATGGAAAACCCATGCCACTCTCAAAACTCATTTCTTCACTACCAATCCACCCTTCAAAAAAGCCTTGCATCTATCGCTTGATGCGAATCATGACTTATTCCGGCTTCTTCTCTCAACAAAATATTACTGAGAATGAGCTAGAGATTGAGTACATGTTAACTGATGTATCTCTATTACTACTTAAGAACAGTCCAATGAGTGTGGCACCACATGTGCACGGGATACTCAGTCCAGATATGATAGATCCATGGCATCAATTCTCTGCTTGGTTAAAAAATGATGATATTACAGCATTTGAAACAACACATGGGATGTCGTTTTGGGATTATCTTGCACGTGACTCCAAATTTAACAACTCATTTAACGAATCAATGGCAAAAGATACTCGATTAGTTTCCGATTTGTTGGTTGAGAAGTGCAAGGGAGTGTTCCATGAAGTTGAGTCATTGGTTGATGTTGGAGGAGGTACGGGGACCATGGCAAAAACTCTTGCCAAATCATTCCCACAAATGGAATGCATTGTGTTTGATCTCCCACATGTTGTTGATGGTTTGCAAGGAAGTGAAAACTTAAACTATGTTGGTGGAGACATGTTTAAAGAAATTCCGCCAACAGATGCAATTTTGTTGAAGTCGATATTGCATGATTGGAATGACGAGGAATGTGTAAAGATATTAAAGAATTGTAAGGATGCAATATCAAAGAAAGGTAAAGGGAAGGTGGTTATCATTGACACGGTGTTAGACAAAGAGAATGGAAACATTAATGAATCAGTTGAAACACAACTCTTCTATGACATGTTGATGATGGTAGTATTCGCAGGAAAAGAGAGAAACGAGAAAGAATGGATTAAGTTGATTTCCTTGGCTGGTTTTAGTGACTACAAGATTACTCCAATTTTAGGATCAAGGTCTATGATTGAGATCTATCCATGA

Protein sequence

>Msa0854730

MSLKCVVDLGIPDIIHNHGKPMPLSKLISSLPIHPSKKPCIYRLMRIMTYSGFFSQQNITENELEIEYMLTDVSLLLLKNSPMSVAPHVHGILSPDMIDPWHQFSAWLKNDDITAFETTHGMSFWDYLARDSKFNNSFNESMAKDTRLVSDLLVEKCKGVFHEVESLVDVGGGTGTMAKTLAKSFPQMECIVFDLPHVVDGLQGSENLNYVGGDMFKEIPPTDAILLKSILHDWNDEECVKILKNCKDAISKKGKGKVVIIDTVLDKENGNINESVETQLFYDMLMMVVFAGKERNEKEWIKLISLAGFSDYKITPILGSRSMIEIYP*