AlfalfaGEDB | Msa0854730

M. sativa cultivar zhongmu-4 / Msa0854730

KEGG: K13262|K16040
GO: GO:0003674 GO:0003824 GO:0006725 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008757 GO:0009058 GO:0009812 GO:0009987 GO:0016740 GO:0016741 GO:0019438 GO:0030761 GO:0032259 GO:0044237 GO:0044249 GO:0071704
PFAM: PF08100.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0854730	QGL04297.1	99.695	328	1	0	1	328	31	358	0.0	676

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0854730	sp\|B0ZB57\|OMT3_HUMLU	58.683	334	132	2	1	328	44	377	1.96e-141	407

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0854730	A0A649WZ66	99.695	328	1	0	1	328	31	358	0.0	676

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0018220	Msa0854730	0.802584	5.233394e-49	-8.615850e-47
Msa0052460	Msa0854730	0.819151	1.349734e-52	-8.615850e-47
Msa0053110	Msa0854730	0.811363	7.269266e-51	-8.615850e-47
Msa0072810	Msa0854730	0.814215	1.725745e-51	-8.615850e-47
Msa0097050	Msa0854730	0.804059	2.590521e-49	-8.615850e-47
Msa0103000	Msa0854730	0.822864	1.885682e-53	-8.615850e-47
Msa0114540	Msa0854730	-0.805975	1.029552e-49	-8.615850e-47
Msa0219190	Msa0854730	0.814960	1.180652e-51	-8.615850e-47
Msa0238890	Msa0854730	0.810152	1.328279e-50	-8.615850e-47
Msa0289890	Msa0854730	0.838316	3.091110e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0854730	MtrunA17_Chr5g0432301	82.927	328	55	1	1	327	32	359	0.0	559
Msa0854730	MtrunA17_Chr5g0432251	82.371	329	57	1	1	328	31	359	0.0	558
Msa0854730	MtrunA17_Chr5g0432211	84.802	329	35	2	1	328	31	345	0.0	557
Msa0854730	MtrunA17_Chr5g0432241	80.851	329	62	1	1	328	32	360	0.0	545
Msa0854730	MtrunA17_Chr5g0432261	69.301	329	72	3	1	328	27	327	4.37e-165	462
Msa0854730	MtrunA17_Chr4g0040621	62.424	330	118	4	1	328	44	369	1.13e-147	420
Msa0854730	MtrunA17_Chr4g0046361	49.550	333	161	6	1	328	31	361	2.83e-108	320
Msa0854730	MtrunA17_Chr4g0014681	50.746	335	156	6	1	328	31	363	2.27e-106	315
Msa0854730	MtrunA17_Chr4g0014561	50.149	335	158	6	1	328	31	363	1.19e-104	310
Msa0854730	MtrunA17_Chr4g0046341	50.000	336	158	6	1	328	31	364	9.69e-101	300

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0854730	AT4G35160.1	35.484	341	200	9	2	327	45	380	6.23e-56	186
Msa0854730	AT4G35150.1	33.731	335	171	9	2	327	31	323	6.59e-52	174
Msa0854730	AT5G54160.1	32.188	320	205	8	1	314	34	347	8.05e-47	162
Msa0854730	AT1G51990.2	31.193	327	211	6	13	328	39	362	9.33e-44	154
Msa0854730	AT1G51990.1	31.193	327	211	6	13	328	39	362	1.24e-43	154
Msa0854730	AT1G77520.1	30.636	346	212	10	1	327	44	380	2.83e-40	145
Msa0854730	AT5G53810.1	29.942	344	218	8	1	327	40	377	5.24e-37	136
Msa0854730	AT1G63140.2	33.528	343	206	11	1	327	44	380	6.21e-37	136
Msa0854730	AT1G63140.4	33.528	343	206	11	1	327	64	400	1.03e-36	136
Msa0854730	AT1G33030.1	31.889	323	204	11	1	314	21	336	1.18e-36	135

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Find 95 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGGGAGACAATGCAG+AGG	0.667854	5_4:-47521348	None:intergenic
AAAGAAAGGTAAAGGGAAGG+TGG	0.580286	5_4:+47520970	Msa0854730:CDS
AAAGAGGGAGACAATGCAGA+GGG	0.658598	5_4:-47521347	None:intergenic
AAAGATATTAAAGAATTGTA+AGG	0.505831	5_4:+47520937	Msa0854730:CDS
AACAAAATTGCATCTGTTGG+CGG	0.431252	5_4:-47520642	None:intergenic
AACACAATGCATTCCATTTG+TGG	0.431379	5_4:-47520540	None:intergenic
AACATTGTGATGATGACAAA+TGG	0.526932	5_4:-47521226	None:intergenic
AAGAGAGAAACGAGAAAGAA+TGG	0.424421	5_4:+47521095	Msa0854730:CDS
AAGCAACATTATCTCCATGT+TGG	0.524077	5_4:-47519903	None:intergenic
AAGGATGCAATATCAAAGAA+AGG	0.545671	5_4:+47520956	Msa0854730:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAAAATGTGATTCCATAAG+TGG	-	chr5_4:47519951-47519970	None:intergenic	30.0%
CAACTCATTTAACGAATCAA+TGG	+	chr5_4:47520389-47520408	Msa0854730:CDS	30.0%
GAAAACTTAAACTATGTTGG+TGG	+	chr5_4:47520600-47520619	Msa0854730:CDS	30.0%
TTCAACAAAATTGCATCTGT+TGG	-	chr5_4:47520648-47520667	None:intergenic	30.0%
CCGTCATTAGTAGAAAAATA+TGG	-	chr5_4:47520776-47520795	None:intergenic	30.0%
GAAAAGAGAACAAACTAAAG+TGG	-	chr5_4:47520836-47520855	None:intergenic	30.0%
AAGGATGCAATATCAAAGAA+AGG	+	chr5_4:47520956-47520975	Msa0854730:CDS	30.0%
GCAATATCAAAGAAAGGTAA+AGG	+	chr5_4:47520962-47520981	Msa0854730:CDS	30.0%
ATAGACCTTGATCCTAAAAT+TGG	-	chr5_4:47521166-47521185	None:intergenic	30.0%
TTTGTCTTAAGAGGATGATT+TGG	+	chr5_4:47521368-47521387	Msa0854730:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	47519786	47521656	47519786	ID=Msa0854730;Name=Msa0854730
chr5_4	mRNA	47519786	47521656	47519786	ID=Msa0854730-mRNA-1;Parent=Msa0854730;Name=Msa0854730-mRNA-1;_AED=0.01;_eAED=0.01;_QI=199\|1\|1\|1\|1\|1\|2\|452\|328
chr5_4	exon	47519786	47520668	47519786	ID=Msa0854730-mRNA-1:exon:14973;Parent=Msa0854730-mRNA-1
chr5_4	exon	47520902	47521656	47520902	ID=Msa0854730-mRNA-1:exon:14974;Parent=Msa0854730-mRNA-1
chr5_4	five_prime_UTR	47519786	47519984	47519786	ID=Msa0854730-mRNA-1:five_prime_utr;Parent=Msa0854730-mRNA-1
chr5_4	CDS	47519985	47520668	47519985	ID=Msa0854730-mRNA-1:cds;Parent=Msa0854730-mRNA-1
chr5_4	CDS	47520902	47521204	47520902	ID=Msa0854730-mRNA-1:cds;Parent=Msa0854730-mRNA-1
chr5_4	three_prime_UTR	47521205	47521656	47521205	ID=Msa0854730-mRNA-1:three_prime_utr;Parent=Msa0854730-mRNA-1

Gene Sequence

>Msa0854730

ATGTCACTTAAATGTGTAGTTGACTTAGGCATACCAGATATCATACACAACCATGGAAAACCCATGCCACTCTCAAAACTCATTTCTTCACTACCAATCCACCCTTCAAAAAAGCCTTGCATCTATCGCTTGATGCGAATCATGACTTATTCCGGCTTCTTCTCTCAACAAAATATTACTGAGAATGAGCTAGAGATTGAGTACATGTTAACTGATGTATCTCTATTACTACTTAAGAACAGTCCAATGAGTGTGGCACCACATGTGCACGGGATACTCAGTCCAGATATGATAGATCCATGGCATCAATTCTCTGCTTGGTTAAAAAATGATGATATTACAGCATTTGAAACAACACATGGGATGTCGTTTTGGGATTATCTTGCACGTGACTCCAAATTTAACAACTCATTTAACGAATCAATGGCAAAAGATACTCGATTAGTTTCCGATTTGTTGGTTGAGAAGTGCAAGGGAGTGTTCCATGAAGTTGAGTCATTGGTTGATGTTGGAGGAGGTACGGGGACCATGGCAAAAACTCTTGCCAAATCATTCCCACAAATGGAATGCATTGTGTTTGATCTCCCACATGTTGTTGATGGTTTGCAAGGAAGTGAAAACTTAAACTATGTTGGTGGAGACATGTTTAAAGAAATTCCGCCAACAGATGCAATTTTGTTGAAGTCGATATTGCATGATTGGAATGACGAGGAATGTGTAAAGATATTAAAGAATTGTAAGGATGCAATATCAAAGAAAGGTAAAGGGAAGGTGGTTATCATTGACACGGTGTTAGACAAAGAGAATGGAAACATTAATGAATCAGTTGAAACACAACTCTTCTATGACATGTTGATGATGGTAGTATTCGCAGGAAAAGAGAGAAACGAGAAAGAATGGATTAAGTTGATTTCCTTGGCTGGTTTTAGTGACTACAAGATTACTCCAATTTTAGGATCAAGGTCTATGATTGAGATCTATCCATGA

Protein sequence

>Msa0854730

MSLKCVVDLGIPDIIHNHGKPMPLSKLISSLPIHPSKKPCIYRLMRIMTYSGFFSQQNITENELEIEYMLTDVSLLLLKNSPMSVAPHVHGILSPDMIDPWHQFSAWLKNDDITAFETTHGMSFWDYLARDSKFNNSFNESMAKDTRLVSDLLVEKCKGVFHEVESLVDVGGGTGTMAKTLAKSFPQMECIVFDLPHVVDGLQGSENLNYVGGDMFKEIPPTDAILLKSILHDWNDEECVKILKNCKDAISKKGKGKVVIIDTVLDKENGNINESVETQLFYDMLMMVVFAGKERNEKEWIKLISLAGFSDYKITPILGSRSMIEIYP*