AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0986900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTAGAATGACATATAAT+TGG 0.218390 6_4:-36236178 None:intergenic
TCATCATGTAAGCCCTCTTT+TGG 0.256450 6_4:-36235635 None:intergenic
ATTAACTTCGATCTCATTCA+TGG 0.296896 6_4:-36235681 None:intergenic
GCCGCCGCTCCTTCTCCTTC+TGG 0.315411 6_4:-36235857 None:intergenic
TAAGATTGTTAGTGTTGTAA+TGG 0.398843 6_4:+36235793 Msa0986900:three_prime_UTR
ACTATAGAAACAAAAGAGAT+TGG 0.417735 6_4:-36233852 None:intergenic
GTAGATGTGGTGTGAGTTGA+AGG 0.428182 6_4:+36235956 Msa0986900:three_prime_UTR
TGTTCTTATCCATGTGTTGA+AGG 0.437532 6_4:+36233620 Msa0986900:three_prime_UTR
AATGGAAATTGAAAACTATA+TGG 0.441067 6_4:+36235811 Msa0986900:three_prime_UTR
TGTTGTGATTCTTATGTTGA+AGG 0.449563 6_4:+36233989 Msa0986900:three_prime_UTR
ATCTCTCGCCGTTCTTCCGT+CGG 0.449935 6_4:-36231775 None:intergenic
GCTCCTTCTCCTTCTGGTTG+CGG 0.498102 6_4:-36235851 None:intergenic
AGGAGAAGGAGCGGCGGCTA+TGG 0.498537 6_4:+36235862 Msa0986900:three_prime_UTR
GTGAAAGAAGTCCTGCGATT+TGG 0.504851 6_4:+36235597 Msa0986900:three_prime_UTR
TGTAATCTTTGATTTGCTGT+TGG 0.505943 6_4:+36235558 Msa0986900:three_prime_UTR
ATATGACATGATTTAGATCA+AGG 0.507574 6_4:+36234051 Msa0986900:three_prime_UTR
AGAAGAACAAGTTGAAATTG+AGG 0.507644 6_4:+36232291 Msa0986900:CDS
CTATAGAAACAAAAGAGATT+GGG 0.515222 6_4:-36233851 None:intergenic
TGTAGTGAAGAGGAGTTGAA+AGG 0.536853 6_4:+36232220 Msa0986900:intron
CCACCGCAACCAGAAGGAGA+AGG 0.537496 6_4:+36235848 Msa0986900:three_prime_UTR
AAATGAAATGTTTGTAGATG+TGG 0.537685 6_4:+36235943 Msa0986900:three_prime_UTR
GGTGATACAGTGTCAGTATG+AGG 0.540392 6_4:+36235726 Msa0986900:three_prime_UTR
TTGGGTTTGAGATTCAATGA+AGG 0.545031 6_4:+36231744 Msa0986900:CDS
TCATGTCATATTGATTTCGC+CGG 0.549696 6_4:-36234039 None:intergenic
TTTATCTTCTATCTTCACCA+TGG 0.556199 6_4:-36233441 None:intergenic
GAAGAGCAGGCACATAATGA+AGG 0.558465 6_4:+36232256 Msa0986900:CDS
AGTCGGATCCGACGGAAGAA+CGG 0.560680 6_4:+36231767 Msa0986900:CDS
AAAGAAGTCCTGCGATTTGG+TGG 0.561416 6_4:+36235600 Msa0986900:three_prime_UTR
AGTTCATCCCACCAAATCGC+AGG 0.565089 6_4:-36235608 None:intergenic
TTGAGATTCAATGAAGGAGT+CGG 0.568945 6_4:+36231750 Msa0986900:CDS
GTGCGGCCACCGCAACCAGA+AGG 0.569602 6_4:+36235842 Msa0986900:three_prime_UTR
TCTTTGATTTGCTGTTGGTG+TGG 0.571367 6_4:+36235563 Msa0986900:three_prime_UTR
CCTTCTCCTTCTGGTTGCGG+TGG 0.584742 6_4:-36235848 None:intergenic
ATGGTGAAGATAGAAGATAA+AGG 0.593300 6_4:+36233443 Msa0986900:CDS
AATTGAAAACTATATGGTGG+TGG 0.600622 6_4:+36235817 Msa0986900:three_prime_UTR
GGATGAACTAGATCCAAAAG+AGG 0.603004 6_4:+36235622 Msa0986900:three_prime_UTR
TGAAAACTATATGGTGGTGG+AGG 0.605543 6_4:+36235820 Msa0986900:three_prime_UTR
TATCTTCAACCTTCAACACA+TGG 0.606912 6_4:-36233629 None:intergenic
AATGAAGGAGTCGGATCCGA+CGG 0.607415 6_4:+36231759 Msa0986900:CDS
AAAGAAAGTTCACTTCTCCA+TGG 0.607605 6_4:+36233424 Msa0986900:CDS
ACTATATGGTGGTGGAGGTG+CGG 0.615339 6_4:+36235825 Msa0986900:three_prime_UTR
GCAACCAGAAGGAGAAGGAG+CGG 0.630733 6_4:+36235853 Msa0986900:three_prime_UTR
ACCAGAAGGAGAAGGAGCGG+CGG 0.633155 6_4:+36235856 Msa0986900:three_prime_UTR
TCATTCATGGAGTTTAGCAG+CGG 0.648637 6_4:-36235668 None:intergenic
GGAAATTGAAAACTATATGG+TGG 0.682928 6_4:+36235814 Msa0986900:three_prime_UTR
GATGAACTAGATCCAAAAGA+GGG 0.688786 6_4:+36235623 Msa0986900:three_prime_UTR
AAGAAGTCCTGCGATTTGGT+GGG 0.691563 6_4:+36235601 Msa0986900:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATTTATCAAGAAAATTA+TGG - chr6_4:36232590-36232609 None:intergenic 10.0%
!! AACATACAAAAATAAAAATA+AGG - chr6_4:36233545-36233564 None:intergenic 10.0%
!! AGAAAATAATAAGAAATATA+AGG - chr6_4:36233906-36233925 None:intergenic 10.0%
!! TCAAAAAAAAAATAAACAAA+AGG - chr6_4:36234253-36234272 None:intergenic 10.0%
!! TTGTAAAAACAAATTATTAA+TGG - chr6_4:36234468-36234487 None:intergenic 10.0%
!!! AAATTCTAGAATTTTTCTAA+AGG - chr6_4:36232410-36232429 None:intergenic 15.0%
!!! TTTAGAAAAATTCTAGAATT+TGG + chr6_4:36232409-36232428 Msa0986900:intron 15.0%
!!! AAATTCTTTTGAAGATTAAT+TGG - chr6_4:36232493-36232512 None:intergenic 15.0%
!! AATCTCTTATAATATAAGAA+AGG + chr6_4:36232702-36232721 Msa0986900:intron 15.0%
!!! GGTAATTTTGTAAAAAAAAT+CGG - chr6_4:36233077-36233096 None:intergenic 15.0%
!! ATAAAATAAACTTGAAGAAA+TGG - chr6_4:36233254-36233273 None:intergenic 15.0%
!! AATAAAAATAAGGAATTGAA+TGG - chr6_4:36233535-36233554 None:intergenic 15.0%
!!! AATTTTTTTCAATCAAAAGA+AGG - chr6_4:36234123-36234142 None:intergenic 15.0%
!!! ATTTTTTTGGATTTTAGTGA+TGG - chr6_4:36232137-36232156 None:intergenic 20.0%
!! TCATCTATTGTGAATTTATT+AGG - chr6_4:36232641-36232660 None:intergenic 20.0%
!! CAAAATTCAAATTTAGAAGA+GGG - chr6_4:36232868-36232887 None:intergenic 20.0%
!! TCAAAATTCAAATTTAGAAG+AGG - chr6_4:36232869-36232888 None:intergenic 20.0%
!!! ATACTGATTTAACATTAGTT+GGG - chr6_4:36232941-36232960 None:intergenic 20.0%
!!! TATACTGATTTAACATTAGT+TGG - chr6_4:36232942-36232961 None:intergenic 20.0%
!! ATATATGTGTAGAAAACAAA+AGG - chr6_4:36233098-36233117 None:intergenic 20.0%
!!! GTTTCATACCTTTTTTTTTT+TGG - chr6_4:36233694-36233713 None:intergenic 20.0%
!!! TTAATTTTGTAAGTCAATCA+TGG - chr6_4:36233930-36233949 None:intergenic 20.0%
!! AAAGAAATACACAATATGAA+TGG - chr6_4:36234087-36234106 None:intergenic 20.0%
!!! TTTTTCCAATTACAATTTGT+TGG - chr6_4:36234158-36234177 None:intergenic 20.0%
!!! TGTCATTCTTTATTTCTAAA+AGG + chr6_4:36234583-36234602 Msa0986900:intron 20.0%
!! TTCTTCTGATAAAACAAAAT+TGG - chr6_4:36234937-36234956 None:intergenic 20.0%
!!! TCTTTTGATAATACTTCTAT+TGG - chr6_4:36235236-36235255 None:intergenic 20.0%
!!! AGTCAAGAAGTTTTTTTTAA+TGG - chr6_4:36235282-36235301 None:intergenic 20.0%
!!! TTAATATCACTTTTCTTGTT+CGG - chr6_4:36235305-36235324 None:intergenic 20.0%
!! ATGATTATGCAAAAAGAAAA+AGG + chr6_4:36235348-36235367 Msa0986900:intron 20.0%
!!! TTTTATAGATCTGATACTAA+TGG - chr6_4:36235419-36235438 None:intergenic 20.0%
!! AATGGAAATTGAAAACTATA+TGG + chr6_4:36235811-36235830 Msa0986900:three_prime_UTR 20.0%
!!! ATGAACGATTTTGAAGATTT+GGG + chr6_4:36231726-36231745 Msa0986900:CDS 25.0%
!!! AGAGAAATATTTTTTGCTGA+GGG + chr6_4:36231921-36231940 Msa0986900:intron 25.0%
! TATATACCCAAAATTTCAGT+TGG + chr6_4:36232036-36232055 Msa0986900:intron 25.0%
!!! TTTTTTACAACACAATCAAG+AGG - chr6_4:36232334-36232353 None:intergenic 25.0%
!!! AGAAAAATTCTAGAATTTGG+TGG + chr6_4:36232412-36232431 Msa0986900:intron 25.0%
! TGAATTTATTAGGAGAATCA+TGG - chr6_4:36232631-36232650 None:intergenic 25.0%
!! GTTCTAGTTAAAAACTCAAA+TGG + chr6_4:36232663-36232682 Msa0986900:intron 25.0%
!!! ACATGCTTACTTTTTTTTGA+GGG - chr6_4:36233212-36233231 None:intergenic 25.0%
! TCAACAAAATCAGAAATGAA+AGG - chr6_4:36233724-36233743 None:intergenic 25.0%
!!! TGTTGTGATTCTTATTTTGA+AGG + chr6_4:36233805-36233824 Msa0986900:intron 25.0%
!!! ATTCTTATTTTGAAGGTTGA+AGG + chr6_4:36233812-36233831 Msa0986900:intron 25.0%
! CTATAGAAACAAAAGAGATT+GGG - chr6_4:36233854-36233873 None:intergenic 25.0%
! ACTATAGAAACAAAAGAGAT+TGG - chr6_4:36233855-36233874 None:intergenic 25.0%
!! AAATGAGACATTTTTGTAAC+CGG + chr6_4:36234020-36234039 Msa0986900:three_prime_UTR 25.0%
! ATATGACATGATTTAGATCA+AGG + chr6_4:36234051-36234070 Msa0986900:three_prime_UTR 25.0%
! AAAAAAAAAATCAACAGCCA+CGG + chr6_4:36234174-36234193 Msa0986900:intron 25.0%
! AAAAATGAAAACAACCATGA+AGG + chr6_4:36234343-36234362 Msa0986900:intron 25.0%
!!! TAATTTGTTTTTACAACCTG+TGG + chr6_4:36234472-36234491 Msa0986900:intron 25.0%
!!! GAATATTTTTAAATAGCCAC+AGG - chr6_4:36234491-36234510 None:intergenic 25.0%
!! CTTACTGCTATTGATTAAAA+AGG - chr6_4:36234973-36234992 None:intergenic 25.0%
!! AATAAAATCCAAGAAAGTGT+AGG + chr6_4:36235018-36235037 Msa0986900:intron 25.0%
!!! ATTTTTTTCCTACACTTTCT+TGG - chr6_4:36235029-36235048 None:intergenic 25.0%
! CTAATAGAAGAATTACCAAA+AGG + chr6_4:36235390-36235409 Msa0986900:intron 25.0%
! AGATCGAAGTTAATCAAAAA+TGG + chr6_4:36235690-36235709 Msa0986900:three_prime_UTR 25.0%
!!! ATCAAAAATGGTGTTTTTAG+TGG + chr6_4:36235702-36235721 Msa0986900:three_prime_UTR 25.0%
! TAAGATTGTTAGTGTTGTAA+TGG + chr6_4:36235793-36235812 Msa0986900:three_prime_UTR 25.0%
! AAATGAAATGTTTGTAGATG+TGG + chr6_4:36235943-36235962 Msa0986900:three_prime_UTR 25.0%
! AAATAAAAAATATGTGGGTG+TGG + chr6_4:36235999-36236018 Msa0986900:three_prime_UTR 25.0%
! TCATGATTTGAAACGAAAAG+AGG + chr6_4:36231812-36231831 Msa0986900:intron 30.0%
! CAATTTGAGATTCAAAGAAG+AGG + chr6_4:36231889-36231908 Msa0986900:intron 30.0%
! AGATTCAAAGAAGAGGATTT+TGG + chr6_4:36231896-36231915 Msa0986900:intron 30.0%
! GATTCAAAGAAGAGGATTTT+GGG + chr6_4:36231897-36231916 Msa0986900:intron 30.0%
! ATTCAAAGAAGAGGATTTTG+GGG + chr6_4:36231898-36231917 Msa0986900:intron 30.0%
!!! GAGAGAAATATTTTTTGCTG+AGG + chr6_4:36231920-36231939 Msa0986900:intron 30.0%
!!! TTGCTGAGGGAAATATTTTT+TGG + chr6_4:36231934-36231953 Msa0986900:intron 30.0%
TAAAGTTGAAAGAGCTTTCA+GGG - chr6_4:36232064-36232083 None:intergenic 30.0%
! TTTCAACTTTAGGAGCTAAA+AGG + chr6_4:36232073-36232092 Msa0986900:intron 30.0%
GACACATAATTTACAACTGT+TGG - chr6_4:36232182-36232201 None:intergenic 30.0%
! TCAATTTTCTTGTAGTGAAG+AGG + chr6_4:36232210-36232229 Msa0986900:intron 30.0%
! AGAAGAACAAGTTGAAATTG+AGG + chr6_4:36232291-36232310 Msa0986900:CDS 30.0%
!!! AAATCCAAGCAACACTTTTT+TGG - chr6_4:36232439-36232458 None:intergenic 30.0%
AGAACATTGAGATCTGAATA+GGG - chr6_4:36233155-36233174 None:intergenic 30.0%
!!! GACATGCTTACTTTTTTTTG+AGG - chr6_4:36233213-36233232 None:intergenic 30.0%
TTTATCTTCTATCTTCACCA+TGG - chr6_4:36233444-36233463 None:intergenic 30.0%
ATGGTGAAGATAGAAGATAA+AGG + chr6_4:36233443-36233462 Msa0986900:CDS 30.0%
! AAGGAATTGAATGGTTAGTT+TGG - chr6_4:36233526-36233545 None:intergenic 30.0%
AGAACAACATAGATCTAACA+TGG - chr6_4:36233606-36233625 None:intergenic 30.0%
CTACGATGCCAAAAAAAAAA+AGG + chr6_4:36233683-36233702 Msa0986900:three_prime_UTR 30.0%
TGTTGTGATTCTTATGTTGA+AGG + chr6_4:36233989-36234008 Msa0986900:three_prime_UTR 30.0%
TAGAGCCAACAAATTGTAAT+TGG + chr6_4:36234150-36234169 Msa0986900:intron 30.0%
TTCTTGTTCATAATCCTTCA+TGG - chr6_4:36234360-36234379 None:intergenic 30.0%
! TGCTAACAAGAAAAGTTTTC+AGG + chr6_4:36235105-36235124 Msa0986900:intron 30.0%
!! TGTAATCTTTGATTTGCTGT+TGG + chr6_4:36235558-36235577 Msa0986900:three_prime_UTR 30.0%
ATTAACTTCGATCTCATTCA+TGG - chr6_4:36235684-36235703 None:intergenic 30.0%
!!! AAAAATGGTGTTTTTAGTGG+CGG + chr6_4:36235705-36235724 Msa0986900:three_prime_UTR 30.0%
GGAAATTGAAAACTATATGG+TGG + chr6_4:36235814-36235833 Msa0986900:three_prime_UTR 30.0%
AATTGAAAACTATATGGTGG+TGG + chr6_4:36235817-36235836 Msa0986900:three_prime_UTR 30.0%
! TTGGGTTTGAGATTCAATGA+AGG + chr6_4:36231744-36231763 Msa0986900:CDS 35.0%
TTGAGATTCAATGAAGGAGT+CGG + chr6_4:36231750-36231769 Msa0986900:CDS 35.0%
CTAAAGTTGAAAGAGCTTTC+AGG - chr6_4:36232065-36232084 None:intergenic 35.0%
CTGAAAGCTCTTTCAACTTT+AGG + chr6_4:36232063-36232082 Msa0986900:intron 35.0%
!!! GGTCGCAATGTGTATTTTTT+TGG - chr6_4:36232150-36232169 None:intergenic 35.0%
!! TACAACTGTTGGTCTTTGAA+GGG - chr6_4:36232171-36232190 None:intergenic 35.0%
!! TTACAACTGTTGGTCTTTGA+AGG - chr6_4:36232172-36232191 None:intergenic 35.0%
!!! AGTTAGTGTTTTAGAAGAGC+AGG + chr6_4:36232243-36232262 Msa0986900:CDS 35.0%
! AAAGTGTGGGAAAAGGTTTT+GGG - chr6_4:36232842-36232861 None:intergenic 35.0%
ATTAGAGAGAGAAGCTTACT+TGG - chr6_4:36233035-36233054 None:intergenic 35.0%
GAGAACATTGAGATCTGAAT+AGG - chr6_4:36233156-36233175 None:intergenic 35.0%
AAAGAAAGTTCACTTCTCCA+TGG + chr6_4:36233424-36233443 Msa0986900:CDS 35.0%
TGTTCTTATCCATGTGTTGA+AGG + chr6_4:36233620-36233639 Msa0986900:three_prime_UTR 35.0%
TATCTTCAACCTTCAACACA+TGG - chr6_4:36233632-36233651 None:intergenic 35.0%
!! TCATGTCATATTGATTTCGC+CGG - chr6_4:36234042-36234061 None:intergenic 35.0%
! GTCATCTTCAATGATTTTGC+AGG + chr6_4:36235174-36235193 Msa0986900:intron 35.0%
! TGGAGTCAATGAATCTGTTT+TGG - chr6_4:36235262-36235281 None:intergenic 35.0%
! TGATACTAATGGTAGCCTTT+TGG - chr6_4:36235408-36235427 None:intergenic 35.0%
GATGAACTAGATCCAAAAGA+GGG + chr6_4:36235623-36235642 Msa0986900:three_prime_UTR 35.0%
! AAGAGGTTTGAGATTCAACG+CGG + chr6_4:36231829-36231848 Msa0986900:intron 40.0%
AAAGTGAGAGAGCTTAGTCT+TGG + chr6_4:36232000-36232019 Msa0986900:intron 40.0%
ACATGCAACAGTTTGGTCAA+GGG - chr6_4:36232113-36232132 None:intergenic 40.0%
TGTAGTGAAGAGGAGTTGAA+AGG + chr6_4:36232220-36232239 Msa0986900:intron 40.0%
!! TGGACCAAAAAAGTGTTGCT+TGG + chr6_4:36232432-36232451 Msa0986900:intron 40.0%
! ACCGATGATTGTTTCTCGAA+AGG + chr6_4:36232464-36232483 Msa0986900:intron 40.0%
GCCTTTCGAGAAACAATCAT+CGG - chr6_4:36232468-36232487 None:intergenic 40.0%
!! GAAAGTGTGGGAAAAGGTTT+TGG - chr6_4:36232843-36232862 None:intergenic 40.0%
! TAGAAGAGGGAAGAAAGTGT+GGG - chr6_4:36232855-36232874 None:intergenic 40.0%
TTAGAAGAGGGAAGAAAGTG+TGG - chr6_4:36232856-36232875 None:intergenic 40.0%
GAGAGAAGCTTACTTGGATA+TGG - chr6_4:36233029-36233048 None:intergenic 40.0%
!! TCTTTGATTTGCTGTTGGTG+TGG + chr6_4:36235563-36235582 Msa0986900:three_prime_UTR 40.0%
GGATGAACTAGATCCAAAAG+AGG + chr6_4:36235622-36235641 Msa0986900:three_prime_UTR 40.0%
! TCATCATGTAAGCCCTCTTT+TGG - chr6_4:36235638-36235657 None:intergenic 40.0%
TCATTCATGGAGTTTAGCAG+CGG - chr6_4:36235671-36235690 None:intergenic 40.0%
!! TGAAAACTATATGGTGGTGG+AGG + chr6_4:36235820-36235839 Msa0986900:three_prime_UTR 40.0%
GGCGAGAGATTTGTGAGATT+TGG + chr6_4:36231788-36231807 Msa0986900:intron 45.0%
! GTTTGAGATTCAACGCGGAA+GGG + chr6_4:36231834-36231853 Msa0986900:intron 45.0%
!! AGAGCTTAGTCTTGGTGAGA+GGG + chr6_4:36232008-36232027 Msa0986900:intron 45.0%
!! AGGGCGCCAACTGAAATTTT+GGG - chr6_4:36232045-36232064 None:intergenic 45.0%
CACATGCAACAGTTTGGTCA+AGG - chr6_4:36232114-36232133 None:intergenic 45.0%
TGATGGCACATGCAACAGTT+TGG - chr6_4:36232120-36232139 None:intergenic 45.0%
GAAGAGCAGGCACATAATGA+AGG + chr6_4:36232256-36232275 Msa0986900:CDS 45.0%
TGCTCGATCCTTCATATCAG+CGG + chr6_4:36232535-36232554 Msa0986900:intron 45.0%
CATCTACACCGCTGATATGA+AGG - chr6_4:36232546-36232565 None:intergenic 45.0%
TGCAGCTTTGCTTGACAAGT+GGG + chr6_4:36232780-36232799 Msa0986900:intron 45.0%
GCAGTTGCTGAATTGACAAG+TGG + chr6_4:36232802-36232821 Msa0986900:intron 45.0%
! AGGGAAGAAAGTGTGGGAAA+AGG - chr6_4:36232849-36232868 None:intergenic 45.0%
TGAACAAACACACTCTACCG+TGG - chr6_4:36234194-36234213 None:intergenic 45.0%
GTGAAAGAAGTCCTGCGATT+TGG + chr6_4:36235597-36235616 Msa0986900:three_prime_UTR 45.0%
! AAAGAAGTCCTGCGATTTGG+TGG + chr6_4:36235600-36235619 Msa0986900:three_prime_UTR 45.0%
!! AAGAAGTCCTGCGATTTGGT+GGG + chr6_4:36235601-36235620 Msa0986900:three_prime_UTR 45.0%
GGTGATACAGTGTCAGTATG+AGG + chr6_4:36235726-36235745 Msa0986900:three_prime_UTR 45.0%
!!! CGGCGGCTATGGTTTTTTAA+GGG + chr6_4:36235873-36235892 Msa0986900:three_prime_UTR 45.0%
! GTAGATGTGGTGTGAGTTGA+AGG + chr6_4:36235956-36235975 Msa0986900:three_prime_UTR 45.0%
!!! TTAGTATTTTTTATTTTTTT+TGG + chr6_4:36231976-36231995 Msa0986900:intron 5.0%
!! TAAAATAAATAAAAAATATG+TGG + chr6_4:36235993-36236012 Msa0986900:three_prime_UTR 5.0%
!! AAAATAAATAAAAAATATGT+GGG + chr6_4:36235994-36236013 Msa0986900:three_prime_UTR 5.0%
AATGAAGGAGTCGGATCCGA+CGG + chr6_4:36231759-36231778 Msa0986900:CDS 50.0%
! GGTTTGAGATTCAACGCGGA+AGG + chr6_4:36231833-36231852 Msa0986900:intron 50.0%
!! GAGAGCTTAGTCTTGGTGAG+AGG + chr6_4:36232007-36232026 Msa0986900:intron 50.0%
!! CAGGGCGCCAACTGAAATTT+TGG - chr6_4:36232046-36232065 None:intergenic 50.0%
! CAGCGTGTATGTGTGCCATT+TGG + chr6_4:36232756-36232775 Msa0986900:intron 50.0%
CAAGCAAAGCTGCAGCCAAA+TGG - chr6_4:36232774-36232793 None:intergenic 50.0%
CTGCAGCTTTGCTTGACAAG+TGG + chr6_4:36232779-36232798 Msa0986900:intron 50.0%
AGTTCATCCCACCAAATCGC+AGG - chr6_4:36235611-36235630 None:intergenic 50.0%
!! ACTATATGGTGGTGGAGGTG+CGG + chr6_4:36235825-36235844 Msa0986900:three_prime_UTR 50.0%
!!! GCGGCGGCTATGGTTTTTTA+AGG + chr6_4:36235872-36235891 Msa0986900:three_prime_UTR 50.0%
AGTCGGATCCGACGGAAGAA+CGG + chr6_4:36231767-36231786 Msa0986900:CDS 55.0%
ATCTCTCGCCGTTCTTCCGT+CGG - chr6_4:36231778-36231797 None:intergenic 55.0%
! GCTCCTTCTCCTTCTGGTTG+CGG - chr6_4:36235854-36235873 None:intergenic 55.0%
GCAACCAGAAGGAGAAGGAG+CGG + chr6_4:36235853-36235872 Msa0986900:three_prime_UTR 55.0%
CTGCACCACAAGGCGACATC+TGG + chr6_4:36232733-36232752 Msa0986900:intron 60.0%
! CCTTCTCCTTCTGGTTGCGG+TGG - chr6_4:36235851-36235870 None:intergenic 60.0%
CCACCGCAACCAGAAGGAGA+AGG + chr6_4:36235848-36235867 Msa0986900:three_prime_UTR 60.0%
ACCAGAAGGAGAAGGAGCGG+CGG + chr6_4:36235856-36235875 Msa0986900:three_prime_UTR 60.0%
GGAGTGCACGCTGCACCACA+AGG + chr6_4:36232723-36232742 Msa0986900:intron 65.0%
CGCTGCCAGATGTCGCCTTG+TGG - chr6_4:36232741-36232760 None:intergenic 65.0%
AGGAGAAGGAGCGGCGGCTA+TGG + chr6_4:36235862-36235881 Msa0986900:three_prime_UTR 65.0%
GTGCGGCCACCGCAACCAGA+AGG + chr6_4:36235842-36235861 Msa0986900:three_prime_UTR 70.0%
GCCGCCGCTCCTTCTCCTTC+TGG - chr6_4:36235860-36235879 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 36231726 36236187 36231726 ID=Msa0986900;Name=Msa0986900
chr6_4 mRNA 36231726 36236187 36231726 ID=Msa0986900-mRNA-1;Parent=Msa0986900;Name=Msa0986900-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|0.66|0.71|0.71|0.33|0.28|7|931|79
chr6_4 exon 36231726 36231799 36231726 ID=Msa0986900-mRNA-1:exon:9143;Parent=Msa0986900-mRNA-1
chr6_4 exon 36232225 36232345 36232225 ID=Msa0986900-mRNA-1:exon:9144;Parent=Msa0986900-mRNA-1
chr6_4 exon 36233418 36233515 36233418 ID=Msa0986900-mRNA-1:exon:9145;Parent=Msa0986900-mRNA-1
chr6_4 exon 36233612 36233704 36233612 ID=Msa0986900-mRNA-1:exon:9146;Parent=Msa0986900-mRNA-1
chr6_4 exon 36233827 36233886 36233827 ID=Msa0986900-mRNA-1:exon:9147;Parent=Msa0986900-mRNA-1
chr6_4 exon 36233981 36234072 36233981 ID=Msa0986900-mRNA-1:exon:9148;Parent=Msa0986900-mRNA-1
chr6_4 exon 36235555 36236187 36235555 ID=Msa0986900-mRNA-1:exon:9149;Parent=Msa0986900-mRNA-1
chr6_4 CDS 36231726 36231799 36231726 ID=Msa0986900-mRNA-1:cds;Parent=Msa0986900-mRNA-1
chr6_4 CDS 36232225 36232345 36232225 ID=Msa0986900-mRNA-1:cds;Parent=Msa0986900-mRNA-1
chr6_4 CDS 36233418 36233462 36233418 ID=Msa0986900-mRNA-1:cds;Parent=Msa0986900-mRNA-1
chr6_4 three_prime_UTR 36233463 36233515 36233463 ID=Msa0986900-mRNA-1:three_prime_utr;Parent=Msa0986900-mRNA-1
chr6_4 three_prime_UTR 36233612 36233704 36233612 ID=Msa0986900-mRNA-1:three_prime_utr;Parent=Msa0986900-mRNA-1
chr6_4 three_prime_UTR 36233827 36233886 36233827 ID=Msa0986900-mRNA-1:three_prime_utr;Parent=Msa0986900-mRNA-1
chr6_4 three_prime_UTR 36233981 36234072 36233981 ID=Msa0986900-mRNA-1:three_prime_utr;Parent=Msa0986900-mRNA-1
chr6_4 three_prime_UTR 36235555 36236187 36235555 ID=Msa0986900-mRNA-1:three_prime_utr;Parent=Msa0986900-mRNA-1
Gene Sequence

>Msa0986900

ATGAACGATTTTGAAGATTTGGGTTTGAGATTCAATGAAGGAGTCGGATCCGACGGAAGAACGGCGAGAGATTTTGAAGAGGAGTTGAAAGGAGTTAGTGTTTTAGAAGAGCAGGCACATAATGAAGGATCTCTAGCTGAAGAAGAACAAGTTGAAATTGAGGATCAATTTGAAGAATATCCTCTTGATTGTGTTAAGCAAAAAGAAAGTTCACTTCTCCATGGTGAAGATAGAAGATAA
Protein sequence

>Msa0986900

MNDFEDLGLRFNEGVGSDGRTARDFEEELKGVSVLEEQAHNEGSLAEEEQVEIEDQFEEYPLDCVKQKESSLLHGEDRR*