AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1038740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1038740 MtrunA17_Chr7g0273521 100.000 176 0 0 1 176 1 176 9.46e-134 371
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1038740 AT3G05000.1 81.818 176 29 2 1 176 1 173 5.50e-107 303

Find 68 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTACTTGTGTTTCTTTAC+AGG 0.160677 7_1:+75719055 Msa1038740:three_prime_UTR
TTCATGGAGATTACGATAAT+TGG 0.205655 7_1:+75718912 Msa1038740:three_prime_UTR
ATTCCCCACTCCAGCAGATT+TGG 0.268780 7_1:-75718778 None:intergenic
TTTCCACTCTATGAAGAAAA+TGG 0.292366 7_1:-75716430 None:intergenic
GCCTCTTCCAGATCCATATA+AGG 0.305068 7_1:-75716323 None:intergenic
GGTAATAGAAATTCCTTATA+TGG 0.320533 7_1:+75716310 Msa1038740:five_prime_UTR
GCCTCGGTTTGGTGATCATT+TGG 0.323253 7_1:+75716955 Msa1038740:CDS
AGCTAATCCTCCAGACAAAA+AGG 0.325927 7_1:-75719102 None:intergenic
ATTAGCTTAAATTTATATAT+CGG 0.328891 7_1:+75719118 Msa1038740:three_prime_UTR
ATTGCATGCATATAAGTGTT+AGG 0.329822 7_1:+75716205 Msa1038740:five_prime_UTR
TTCCCATGTGGGATCATTAG+AGG 0.342467 7_1:+75717294 Msa1038740:CDS
GATCGAAGCCATTGGTTATC+AGG 0.347972 7_1:+75716559 Msa1038740:CDS
ATCCTGCGAGCAGCAAGTTC+AGG 0.371750 7_1:-75716539 None:intergenic
AATGTTATATCCCTCTTGTA+TGG 0.381414 7_1:+75719001 Msa1038740:three_prime_UTR
TTCCCCACTCCAGCAGATTT+GGG 0.382004 7_1:-75718777 None:intergenic
GATATACGATGGAGCGGCCT+CGG 0.415640 7_1:+75716939 Msa1038740:intron
AGAAATTCCTTATATGGATC+TGG 0.425362 7_1:+75716316 Msa1038740:five_prime_UTR
TAGAGGAGCTCTTTCCAACT+TGG 0.425869 7_1:+75717311 Msa1038740:CDS
TGCACCTACGGCTGAAAGTA+AGG 0.438829 7_1:+75717242 Msa1038740:CDS
AGCAGCAAGTTCAGGTTTGT+TGG 0.441318 7_1:-75716531 None:intergenic
GCTCACATAGTCACATACTA+TGG 0.452806 7_1:+75718941 Msa1038740:three_prime_UTR
GGCAATTAAGTTCATTTGCA+AGG 0.458740 7_1:+75716979 Msa1038740:CDS
GGCTGAAAGTAAGGCAGCAC+AGG 0.488115 7_1:+75717251 Msa1038740:CDS
ACGATGGAGCGGCCTCGGTT+TGG 0.496970 7_1:+75716944 Msa1038740:CDS
ATAAATTTCCGTGGCTTGCA+CGG 0.497008 7_1:+75717169 Msa1038740:CDS
AGAGGAGCTCTTTCCAACTT+GGG 0.498177 7_1:+75717312 Msa1038740:CDS
TTGCATGCATATAAGTGTTA+GGG 0.516454 7_1:+75716206 Msa1038740:five_prime_UTR
TCCGTGGCTTGCACGGATGT+CGG 0.520572 7_1:+75717176 Msa1038740:CDS
TCGGTTTGGTGATCATTTGG+AGG 0.520959 7_1:+75716958 Msa1038740:CDS
AACCTGAACTTGCTGCTCGC+AGG 0.521375 7_1:+75716537 Msa1038740:CDS
TGCATCTTTATTTCCCATGT+GGG 0.524113 7_1:+75717283 Msa1038740:CDS
AAAGAAACACAAGTAACAAA+AGG 0.525618 7_1:-75719050 None:intergenic
ACAACTTGAAAACTAACCAT+AGG 0.528003 7_1:+75717035 Msa1038740:CDS
TCCTTATATGGATCTGGAAG+AGG 0.529114 7_1:+75716322 Msa1038740:five_prime_UTR
CATTTGTATTACTGATGTGA+GGG 0.543685 7_1:+75718698 Msa1038740:three_prime_UTR
TATGGATCTGGAAGAGGCAA+AGG 0.546494 7_1:+75716328 Msa1038740:five_prime_UTR
CAACTTGAAAACTAACCATA+GGG 0.547452 7_1:+75717036 Msa1038740:CDS
TGATAATGTTAGTTCAGTTG+AGG 0.553497 7_1:+75717212 Msa1038740:CDS
TGCACAGGGAATTCCCAAGT+TGG 0.556321 7_1:-75717325 None:intergenic
TGAGGATTATTCTGCACCTA+CGG 0.558019 7_1:+75717230 Msa1038740:CDS
GCTGCCTTACTTTCAGCCGT+AGG 0.558260 7_1:-75717246 None:intergenic
TTCATAGAGTGGAAATGGTG+AGG 0.561482 7_1:+75716438 Msa1038740:exon
ACCGACATCCGTGCAAGCCA+CGG 0.566327 7_1:-75717177 None:intergenic
TATGTCTGCAGAAACTGCAC+AGG 0.568585 7_1:-75717340 None:intergenic
GAACTAACATTATCAGTGGA+TGG 0.571137 7_1:-75717204 None:intergenic
TCATTTGTATTACTGATGTG+AGG 0.576744 7_1:+75718697 Msa1038740:three_prime_UTR
GGGGATACGCACATGCTGGA+AGG 0.585352 7_1:-75717368 None:intergenic
TTGCGTTGATAGCTTGATGA+CGG 0.587460 7_1:+75716475 Msa1038740:CDS
GTGCACTGTTGAAAGACAAT+GGG 0.593198 7_1:+75718963 Msa1038740:three_prime_UTR
TAGCATTCGTTCCATACAAG+AGG 0.596722 7_1:-75719012 None:intergenic
TCATAGAGTGGAAATGGTGA+GGG 0.597224 7_1:+75716439 Msa1038740:exon
TGCAAGATAATAAATTTCCG+TGG 0.603647 7_1:+75717160 Msa1038740:CDS
TCGGTCAACAGCTCTCTGAA+AGG 0.608978 7_1:+75716582 Msa1038740:CDS
ATGCATCTTTATTTCCCATG+TGG 0.611221 7_1:+75717282 Msa1038740:CDS
GCTCGCAGGATCGAAGCCAT+TGG 0.612045 7_1:+75716551 Msa1038740:CDS
GAAGCCATTGGTTATCAGGT+CGG 0.622295 7_1:+75716563 Msa1038740:CDS
GCTCCTCTAATGATCCCACA+TGG 0.623231 7_1:-75717297 None:intergenic
AACTGAACTAACATTATCAG+TGG 0.624516 7_1:-75717208 None:intergenic
TTGACCGACCTGATAACCAA+TGG 0.626958 7_1:-75716567 None:intergenic
TGTGTAATTCAGATATACGA+TGG 0.627817 7_1:+75716928 Msa1038740:intron
TGATAGCTTGATGACGGAGA+TGG 0.641800 7_1:+75716481 Msa1038740:CDS
ATTTGTATTACTGATGTGAG+GGG 0.649119 7_1:+75718699 Msa1038740:three_prime_UTR
GGTGCACTGTTGAAAGACAA+TGG 0.652678 7_1:+75718962 Msa1038740:three_prime_UTR
CTCCTCTAATGATCCCACAT+GGG 0.669983 7_1:-75717296 None:intergenic
AGCATTCGTTCCATACAAGA+GGG 0.672193 7_1:-75719011 None:intergenic
AATTCAGATATACGATGGAG+CGG 0.678919 7_1:+75716933 Msa1038740:intron
TCCAAATGATCACCAAACCG+AGG 0.695841 7_1:-75716956 None:intergenic
ATGTCTGCAGAAACTGCACA+GGG 0.724210 7_1:-75717339 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAAAAAATAAACTTC+AGG - chr7_1:75717941-75717960 None:intergenic 10.0%
!!! ATTAGCTTAAATTTATATAT+CGG + chr7_1:75719118-75719137 Msa1038740:three_prime_UTR 10.0%
!!! TTCTTCATGTTAATTATTTT+TGG + chr7_1:75716686-75716705 Msa1038740:intron 15.0%
!! TTGAATGTATAGATATATAT+TGG + chr7_1:75717072-75717091 Msa1038740:intron 15.0%
!!! TGAATAATTTTATTCCATAA+TGG - chr7_1:75718055-75718074 None:intergenic 15.0%
!! ATGGAATAAAATTATTCAAA+AGG + chr7_1:75718057-75718076 Msa1038740:intron 15.0%
!!! AGTAATTTCCTTTTCTTTTT+TGG + chr7_1:75716235-75716254 Msa1038740:five_prime_UTR 20.0%
!!! TCATGTTAATTATTTTTGGA+AGG + chr7_1:75716690-75716709 Msa1038740:intron 20.0%
!! TCATGAAATTGTAAACAAAT+TGG + chr7_1:75716735-75716754 Msa1038740:intron 20.0%
!!! TTTTCATTTTTAGTGTTTTC+TGG + chr7_1:75717454-75717473 Msa1038740:intron 20.0%
!!! AAAGTTTCTTTGTTAGATTT+TGG + chr7_1:75717613-75717632 Msa1038740:intron 20.0%
!! TGCTCTAATATTTATAAACA+TGG - chr7_1:75718284-75718303 None:intergenic 20.0%
!! AAATATGTAAACAAGACAAA+TGG - chr7_1:75718606-75718625 None:intergenic 20.0%
!!! ATATCCTTTTACACATTATT+TGG + chr7_1:75718637-75718656 Msa1038740:intron 20.0%
!!! ATGAAGTGTTTTGATAAATT+AGG + chr7_1:75718864-75718883 Msa1038740:intron 20.0%
!!! TTTTGATAAATTAGGACTTT+TGG + chr7_1:75718872-75718891 Msa1038740:intron 20.0%
!! TCCTTTCTTTTGTCAAAAAA+AGG - chr7_1:75716267-75716286 None:intergenic 25.0%
!!! TTTTTTGACAAAAGAAAGGA+AGG + chr7_1:75716267-75716286 Msa1038740:five_prime_UTR 25.0%
! GGTAATAGAAATTCCTTATA+TGG + chr7_1:75716310-75716329 Msa1038740:five_prime_UTR 25.0%
! GAAATAGAATAATCCTACTA+TGG - chr7_1:75716669-75716688 None:intergenic 25.0%
!! ATTTTCTCGAATTACTACAT+AGG - chr7_1:75716803-75716822 None:intergenic 25.0%
!!! ACACAAGAGAAGTTTTTTTA+AGG + chr7_1:75717416-75717435 Msa1038740:intron 25.0%
! TGTGAATAATTTGATCTGTT+TGG + chr7_1:75717505-75717524 Msa1038740:intron 25.0%
! TGTTTGGAAATGTTTAAACA+GGG + chr7_1:75717521-75717540 Msa1038740:intron 25.0%
! TTTATTGATCAAATCTCTGT+TGG + chr7_1:75717643-75717662 Msa1038740:intron 25.0%
! TTATTGATCAAATCTCTGTT+GGG + chr7_1:75717644-75717663 Msa1038740:intron 25.0%
! CTATATATCTCTTTCCATTA+TGG + chr7_1:75718038-75718057 Msa1038740:intron 25.0%
!! GAACTTATCAAGCTTTTATA+CGG - chr7_1:75718679-75718698 None:intergenic 25.0%
!! ACTATCAAATACTATTGACT+TGG + chr7_1:75718821-75718840 Msa1038740:intron 25.0%
! ATTTATCAAAACACTTCATC+TGG - chr7_1:75718863-75718882 None:intergenic 25.0%
! AAAGAAACACAAGTAACAAA+AGG - chr7_1:75719053-75719072 None:intergenic 25.0%
ATTGCATGCATATAAGTGTT+AGG + chr7_1:75716205-75716224 Msa1038740:five_prime_UTR 30.0%
TTGCATGCATATAAGTGTTA+GGG + chr7_1:75716206-75716225 Msa1038740:five_prime_UTR 30.0%
!!! GCCTTTTTTTGACAAAAGAA+AGG + chr7_1:75716263-75716282 Msa1038740:five_prime_UTR 30.0%
! GAAGGTGCATTAGTAAAAAA+GGG + chr7_1:75716285-75716304 Msa1038740:five_prime_UTR 30.0%
AAGGTGCATTAGTAAAAAAG+GGG + chr7_1:75716286-75716305 Msa1038740:five_prime_UTR 30.0%
AGAAATTCCTTATATGGATC+TGG + chr7_1:75716316-75716335 Msa1038740:five_prime_UTR 30.0%
TTTCCACTCTATGAAGAAAA+TGG - chr7_1:75716433-75716452 None:intergenic 30.0%
! TTTTCTTCATAGAGTGGAAA+TGG + chr7_1:75716433-75716452 Msa1038740:exon 30.0%
GATGAAGAAAGAGAGAATTA+GGG - chr7_1:75716622-75716641 None:intergenic 30.0%
AGATGAAGAAAGAGAGAATT+AGG - chr7_1:75716623-75716642 None:intergenic 30.0%
TGTGTAATTCAGATATACGA+TGG + chr7_1:75716928-75716947 Msa1038740:intron 30.0%
! AGTTCATTTGCAAGGATTTT+TGG + chr7_1:75716987-75717006 Msa1038740:CDS 30.0%
ACAACTTGAAAACTAACCAT+AGG + chr7_1:75717035-75717054 Msa1038740:CDS 30.0%
CAACTTGAAAACTAACCATA+GGG + chr7_1:75717036-75717055 Msa1038740:CDS 30.0%
! TTTTTGTGTTCTGATTTCGA+TGG + chr7_1:75717097-75717116 Msa1038740:intron 30.0%
TGCAAGATAATAAATTTCCG+TGG + chr7_1:75717160-75717179 Msa1038740:CDS 30.0%
AACTGAACTAACATTATCAG+TGG - chr7_1:75717211-75717230 None:intergenic 30.0%
! TGATAATGTTAGTTCAGTTG+AGG + chr7_1:75717212-75717231 Msa1038740:CDS 30.0%
CTGTTTGGAAATGTTTAAAC+AGG + chr7_1:75717520-75717539 Msa1038740:intron 30.0%
! TAGACTAGTTCATACTCAAT+GGG + chr7_1:75717546-75717565 Msa1038740:intron 30.0%
TCTATGGTAAAAAACCAAGA+AGG + chr7_1:75717747-75717766 Msa1038740:intron 30.0%
CTATGGTAAAAAACCAAGAA+GGG + chr7_1:75717748-75717767 Msa1038740:intron 30.0%
TATGGTAAAAAACCAAGAAG+GGG + chr7_1:75717749-75717768 Msa1038740:intron 30.0%
!! ACTTCAGGTTTATTTCTAAC+TGG - chr7_1:75717926-75717945 None:intergenic 30.0%
CACGAACATAACTTCAATAT+TGG + chr7_1:75718334-75718353 Msa1038740:intron 30.0%
AACATAACTTCAATATTGGC+AGG + chr7_1:75718338-75718357 Msa1038740:intron 30.0%
TCACATATTCAGTTTATACC+TGG + chr7_1:75718426-75718445 Msa1038740:intron 30.0%
CTGTATTTGACACAAAATGA+AGG - chr7_1:75718536-75718555 None:intergenic 30.0%
TCATTTGTATTACTGATGTG+AGG + chr7_1:75718697-75718716 Msa1038740:three_prime_UTR 30.0%
CATTTGTATTACTGATGTGA+GGG + chr7_1:75718698-75718717 Msa1038740:three_prime_UTR 30.0%
ATTTGTATTACTGATGTGAG+GGG + chr7_1:75718699-75718718 Msa1038740:three_prime_UTR 30.0%
!!! ATTAGGACTTTTGGTTCTTT+TGG + chr7_1:75718881-75718900 Msa1038740:intron 30.0%
TTCATGGAGATTACGATAAT+TGG + chr7_1:75718912-75718931 Msa1038740:three_prime_UTR 30.0%
AATGTTATATCCCTCTTGTA+TGG + chr7_1:75719001-75719020 Msa1038740:three_prime_UTR 30.0%
! TGTTACTTGTGTTTCTTTAC+AGG + chr7_1:75719055-75719074 Msa1038740:three_prime_UTR 30.0%
! TTTTCCTTTTCCTTTTTGTC+TGG + chr7_1:75719092-75719111 Msa1038740:three_prime_UTR 30.0%
GGCAAAGACCAAAAAAGAAA+AGG - chr7_1:75716246-75716265 None:intergenic 35.0%
! GGAAGGTGCATTAGTAAAAA+AGG + chr7_1:75716284-75716303 Msa1038740:five_prime_UTR 35.0%
! CTTCCATTTTCTTCATAGAG+TGG + chr7_1:75716427-75716446 Msa1038740:five_prime_UTR 35.0%
AGAAAGAGAGAATTAGGGTT+TGG - chr7_1:75716617-75716636 None:intergenic 35.0%
AATTCAGATATACGATGGAG+CGG + chr7_1:75716933-75716952 Msa1038740:intron 35.0%
GGCAATTAAGTTCATTTGCA+AGG + chr7_1:75716979-75716998 Msa1038740:CDS 35.0%
! TCGATGGTTGTGTTGTTATT+TGG + chr7_1:75717113-75717132 Msa1038740:intron 35.0%
GAACTAACATTATCAGTGGA+TGG - chr7_1:75717207-75717226 None:intergenic 35.0%
! ATGCATCTTTATTTCCCATG+TGG + chr7_1:75717282-75717301 Msa1038740:CDS 35.0%
! TGCATCTTTATTTCCCATGT+GGG + chr7_1:75717283-75717302 Msa1038740:CDS 35.0%
! CTAGACTAGTTCATACTCAA+TGG + chr7_1:75717545-75717564 Msa1038740:intron 35.0%
! CATCATTGACTGATTCTCTA+TGG + chr7_1:75717731-75717750 Msa1038740:intron 35.0%
ACAAAACTATCTCTGTGATC+AGG + chr7_1:75717864-75717883 Msa1038740:intron 35.0%
!! CAGGTTTATTTCTAACTGGA+GGG - chr7_1:75717922-75717941 None:intergenic 35.0%
!! TCAGGTTTATTTCTAACTGG+AGG - chr7_1:75717923-75717942 None:intergenic 35.0%
!! AAAAGGTGCTGATAGTAAAG+TGG + chr7_1:75718074-75718093 Msa1038740:intron 35.0%
TTCACAGTTCACACATAAGA+GGG + chr7_1:75718228-75718247 Msa1038740:intron 35.0%
! ATTAGAGCATCTCATGATTC+AGG + chr7_1:75718295-75718314 Msa1038740:intron 35.0%
! TATTAGCCTAAGATGCATAC+AGG - chr7_1:75718364-75718383 None:intergenic 35.0%
CAGTCTGTCATGAATAGATA+GGG - chr7_1:75718491-75718510 None:intergenic 35.0%
ACAGTCTGTCATGAATAGAT+AGG - chr7_1:75718492-75718511 None:intergenic 35.0%
CCTGCCAAATAATGTGTAAA+AGG - chr7_1:75718644-75718663 None:intergenic 35.0%
! CCTTTTACACATTATTTGGC+AGG + chr7_1:75718641-75718660 Msa1038740:intron 35.0%
!! TGACTTGGTTGTATAGAAAG+AGG + chr7_1:75718836-75718855 Msa1038740:intron 35.0%
GTGCATTAGTAAAAAAGGGG+TGG + chr7_1:75716289-75716308 Msa1038740:five_prime_UTR 40.0%
TCCTTATATGGATCTGGAAG+AGG + chr7_1:75716322-75716341 Msa1038740:five_prime_UTR 40.0%
TTCATAGAGTGGAAATGGTG+AGG + chr7_1:75716438-75716457 Msa1038740:exon 40.0%
TCATAGAGTGGAAATGGTGA+GGG + chr7_1:75716439-75716458 Msa1038740:exon 40.0%
!! TTGCGTTGATAGCTTGATGA+CGG + chr7_1:75716475-75716494 Msa1038740:CDS 40.0%
ATGGTGTGTGATAACAGATC+TGG - chr7_1:75716650-75716669 None:intergenic 40.0%
CTGTTATCACACACCATAGT+AGG + chr7_1:75716653-75716672 Msa1038740:intron 40.0%
TGAATGTAGTCACATGTGTC+AGG + chr7_1:75716831-75716850 Msa1038740:intron 40.0%
GAATGTAGTCACATGTGTCA+GGG + chr7_1:75716832-75716851 Msa1038740:intron 40.0%
! GTCAGATAGGTTATGATGGA+TGG + chr7_1:75716854-75716873 Msa1038740:intron 40.0%
!!! TTGCAAGGATTTTTGGTCTG+AGG + chr7_1:75716994-75717013 Msa1038740:CDS 40.0%
AACGAATTCAACTCACCCTA+TGG - chr7_1:75717054-75717073 None:intergenic 40.0%
!! TTGTTATTTGGTTCTGCTGC+AGG + chr7_1:75717125-75717144 Msa1038740:intron 40.0%
!! TGTTATTTGGTTCTGCTGCA+GGG + chr7_1:75717126-75717145 Msa1038740:intron 40.0%
! ATAAATTTCCGTGGCTTGCA+CGG + chr7_1:75717169-75717188 Msa1038740:CDS 40.0%
TGAGGATTATTCTGCACCTA+CGG + chr7_1:75717230-75717249 Msa1038740:CDS 40.0%
CATGGGGTTTCTACTATTGT+GGG + chr7_1:75717782-75717801 Msa1038740:intron 40.0%
ATGGGGTTTCTACTATTGTG+GGG + chr7_1:75717783-75717802 Msa1038740:intron 40.0%
TACTATTGTGGGGTCTGATA+TGG + chr7_1:75717793-75717812 Msa1038740:intron 40.0%
!!! AGATAGTTTTGTGACCCTCT+CGG - chr7_1:75717856-75717875 None:intergenic 40.0%
ATACTGAAGCAACCAATGCA+GGG - chr7_1:75718128-75718147 None:intergenic 40.0%
TATACTGAAGCAACCAATGC+AGG - chr7_1:75718129-75718148 None:intergenic 40.0%
!! TGCATTGGTTGCTTCAGTAT+AGG + chr7_1:75718128-75718147 Msa1038740:intron 40.0%
CTTCACAGTTCACACATAAG+AGG + chr7_1:75718227-75718246 Msa1038740:intron 40.0%
AAGAGGGTCTAGGCATTTAT+AGG + chr7_1:75718244-75718263 Msa1038740:intron 40.0%
ACAATGACAACATGTACTCC+AGG - chr7_1:75718447-75718466 None:intergenic 40.0%
AAGGAATTTGCAAGCAGCAA+TGG - chr7_1:75718517-75718536 None:intergenic 40.0%
AGAGCAAGAACAAAGATGCT+TGG - chr7_1:75718561-75718580 None:intergenic 40.0%
CAAGACAAATGGTCATGCAT+TGG - chr7_1:75718595-75718614 None:intergenic 40.0%
!! TCTTTTGGCTGCAGATTTCA+TGG + chr7_1:75718896-75718915 Msa1038740:intron 40.0%
GCTCACATAGTCACATACTA+TGG + chr7_1:75718941-75718960 Msa1038740:three_prime_UTR 40.0%
GTGCACTGTTGAAAGACAAT+GGG + chr7_1:75718963-75718982 Msa1038740:three_prime_UTR 40.0%
AGCATTCGTTCCATACAAGA+GGG - chr7_1:75719014-75719033 None:intergenic 40.0%
TAGCATTCGTTCCATACAAG+AGG - chr7_1:75719015-75719034 None:intergenic 40.0%
! TCCTTTTCCTTTTTGTCTGG+AGG + chr7_1:75719095-75719114 Msa1038740:three_prime_UTR 40.0%
TCCTCCAGACAAAAAGGAAA+AGG - chr7_1:75719099-75719118 None:intergenic 40.0%
AGCTAATCCTCCAGACAAAA+AGG - chr7_1:75719105-75719124 None:intergenic 40.0%
GCCTCTTCCAGATCCATATA+AGG - chr7_1:75716326-75716345 None:intergenic 45.0%
TATGGATCTGGAAGAGGCAA+AGG + chr7_1:75716328-75716347 Msa1038740:five_prime_UTR 45.0%
!! TGATAGCTTGATGACGGAGA+TGG + chr7_1:75716481-75716500 Msa1038740:CDS 45.0%
! AGCAGCAAGTTCAGGTTTGT+TGG - chr7_1:75716534-75716553 None:intergenic 45.0%
! GATCGAAGCCATTGGTTATC+AGG + chr7_1:75716559-75716578 Msa1038740:CDS 45.0%
! GAAGCCATTGGTTATCAGGT+CGG + chr7_1:75716563-75716582 Msa1038740:CDS 45.0%
TTGACCGACCTGATAACCAA+TGG - chr7_1:75716570-75716589 None:intergenic 45.0%
! TCAACAGCTCTCTGAAAGGT+TGG + chr7_1:75716586-75716605 Msa1038740:intron 45.0%
ACTACATAGGTCGTGCTTCA+TGG - chr7_1:75716790-75716809 None:intergenic 45.0%
CAGGGTCAGATAGGTTATGA+TGG + chr7_1:75716850-75716869 Msa1038740:intron 45.0%
TCCAAATGATCACCAAACCG+AGG - chr7_1:75716959-75716978 None:intergenic 45.0%
!! TCGGTTTGGTGATCATTTGG+AGG + chr7_1:75716958-75716977 Msa1038740:CDS 45.0%
TTCCCATGTGGGATCATTAG+AGG + chr7_1:75717294-75717313 Msa1038740:CDS 45.0%
CTCCTCTAATGATCCCACAT+GGG - chr7_1:75717299-75717318 None:intergenic 45.0%
TAGAGGAGCTCTTTCCAACT+TGG + chr7_1:75717311-75717330 Msa1038740:CDS 45.0%
AGAGGAGCTCTTTCCAACTT+GGG + chr7_1:75717312-75717331 Msa1038740:CDS 45.0%
ATGTCTGCAGAAACTGCACA+GGG - chr7_1:75717342-75717361 None:intergenic 45.0%
TATGTCTGCAGAAACTGCAC+AGG - chr7_1:75717343-75717362 None:intergenic 45.0%
GGTAAAAAACCAAGAAGGGG+CGG + chr7_1:75717752-75717771 Msa1038740:intron 45.0%
GCATGGGGTTTCTACTATTG+TGG + chr7_1:75717781-75717800 Msa1038740:intron 45.0%
!! TTTAGCCTTAGCCCTATGAG+TGG + chr7_1:75717826-75717845 Msa1038740:intron 45.0%
!!! GATAGTTTTGTGACCCTCTC+GGG - chr7_1:75717855-75717874 None:intergenic 45.0%
TACTGAAGCAACCAATGCAG+GGG - chr7_1:75718127-75718146 None:intergenic 45.0%
GTTCACACATAAGAGGGTCT+AGG + chr7_1:75718234-75718253 Msa1038740:intron 45.0%
! CTCTTTTGTCCCAAATCTGC+TGG + chr7_1:75718768-75718787 Msa1038740:intron 45.0%
GGTGCACTGTTGAAAGACAA+TGG + chr7_1:75718962-75718981 Msa1038740:three_prime_UTR 45.0%
TCGGTCAACAGCTCTCTGAA+AGG + chr7_1:75716582-75716601 Msa1038740:CDS 50.0%
CACATGTGTCAGGGTCAGAT+AGG + chr7_1:75716841-75716860 Msa1038740:intron 50.0%
!! GCCTCGGTTTGGTGATCATT+TGG + chr7_1:75716955-75716974 Msa1038740:CDS 50.0%
TGCACCTACGGCTGAAAGTA+AGG + chr7_1:75717242-75717261 Msa1038740:CDS 50.0%
GCTCCTCTAATGATCCCACA+TGG - chr7_1:75717300-75717319 None:intergenic 50.0%
TGCACAGGGAATTCCCAAGT+TGG - chr7_1:75717328-75717347 None:intergenic 50.0%
TGTGTGAGAGAGAGGGAGAT+GGG - chr7_1:75717393-75717412 None:intergenic 50.0%
GTGTGTGTGTGTGAGAGAGA+GGG - chr7_1:75717400-75717419 None:intergenic 50.0%
TGTGTGTGTGTGTGAGAGAG+AGG - chr7_1:75717401-75717420 None:intergenic 50.0%
TCTAACTGGAGGGTGAGACT+TGG - chr7_1:75717912-75717931 None:intergenic 50.0%
GGGTCTAGGCATTTATAGGC+TGG + chr7_1:75718248-75718267 Msa1038740:intron 50.0%
TTGTCCCAAATCTGCTGGAG+TGG + chr7_1:75718773-75718792 Msa1038740:intron 50.0%
TGTCCCAAATCTGCTGGAGT+GGG + chr7_1:75718774-75718793 Msa1038740:intron 50.0%
TTCCCCACTCCAGCAGATTT+GGG - chr7_1:75718780-75718799 None:intergenic 50.0%
ATTCCCCACTCCAGCAGATT+TGG - chr7_1:75718781-75718800 None:intergenic 50.0%
!! AACCTGAACTTGCTGCTCGC+AGG + chr7_1:75716537-75716556 Msa1038740:CDS 55.0%
ATCCTGCGAGCAGCAAGTTC+AGG - chr7_1:75716542-75716561 None:intergenic 55.0%
GATATACGATGGAGCGGCCT+CGG + chr7_1:75716939-75716958 Msa1038740:intron 55.0%
GCTGCCTTACTTTCAGCCGT+AGG - chr7_1:75717249-75717268 None:intergenic 55.0%
GGCTGAAAGTAAGGCAGCAC+AGG + chr7_1:75717251-75717270 Msa1038740:CDS 55.0%
TGTGAGAGAGAGGGAGATGG+GGG - chr7_1:75717391-75717410 None:intergenic 55.0%
GTGTGAGAGAGAGGGAGATG+GGG - chr7_1:75717392-75717411 None:intergenic 55.0%
GTGTGTGAGAGAGAGGGAGA+TGG - chr7_1:75717394-75717413 None:intergenic 55.0%
TGCACTAAACCGCCCCTTCT+TGG - chr7_1:75717764-75717783 None:intergenic 55.0%
AGAAGGGGCGGTTTAGTGCA+TGG + chr7_1:75717764-75717783 Msa1038740:intron 55.0%
GAAGGGGCGGTTTAGTGCAT+GGG + chr7_1:75717765-75717784 Msa1038740:intron 55.0%
AAGGGGCGGTTTAGTGCATG+GGG + chr7_1:75717766-75717785 Msa1038740:intron 55.0%
GGTCTCCACTCATAGGGCTA+AGG - chr7_1:75717834-75717853 None:intergenic 55.0%
CTCTCGGGTCTCCACTCATA+GGG - chr7_1:75717840-75717859 None:intergenic 55.0%
CTATGAGTGGAGACCCGAGA+GGG + chr7_1:75717839-75717858 Msa1038740:intron 55.0%
GATTCTACCAGCCCCTGCAT+TGG + chr7_1:75718113-75718132 Msa1038740:intron 55.0%
GGCAGGCCTGTATGCATCTT+AGG + chr7_1:75718355-75718374 Msa1038740:intron 55.0%
GTCCCAAATCTGCTGGAGTG+GGG + chr7_1:75718775-75718794 Msa1038740:intron 55.0%
GCTCGCAGGATCGAAGCCAT+TGG + chr7_1:75716551-75716570 Msa1038740:CDS 60.0%
! TCCGTGGCTTGCACGGATGT+CGG + chr7_1:75717176-75717195 Msa1038740:CDS 60.0%
ACCGACATCCGTGCAAGCCA+CGG - chr7_1:75717180-75717199 None:intergenic 60.0%
GGGGATACGCACATGCTGGA+AGG - chr7_1:75717371-75717390 None:intergenic 60.0%
ATGGGGGGATACGCACATGC+TGG - chr7_1:75717375-75717394 None:intergenic 60.0%
GTGAGAGAGAGGGAGATGGG+GGG - chr7_1:75717390-75717409 None:intergenic 60.0%
CCTCTCGGGTCTCCACTCAT+AGG - chr7_1:75717841-75717860 None:intergenic 60.0%
CCTATGAGTGGAGACCCGAG+AGG + chr7_1:75717838-75717857 Msa1038740:intron 60.0%
GAAGCAACCAATGCAGGGGC+TGG - chr7_1:75718123-75718142 None:intergenic 60.0%
ACGATGGAGCGGCCTCGGTT+TGG + chr7_1:75716944-75716963 Msa1038740:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 75716205 75719155 75716205 ID=Msa1038740;Name=Msa1038740
chr7_1 mRNA 75716205 75719155 75716205 ID=Msa1038740-mRNA-1;Parent=Msa1038740;Name=Msa1038740-mRNA-1;_AED=0.07;_eAED=0.07;_QI=247|1|1|1|0.4|0.5|6|323|176
chr7_1 exon 75716205 75716603 75716205 ID=Msa1038740-mRNA-1:exon:19400;Parent=Msa1038740-mRNA-1
chr7_1 exon 75716940 75717057 75716940 ID=Msa1038740-mRNA-1:exon:19401;Parent=Msa1038740-mRNA-1
chr7_1 exon 75717147 75717378 75717147 ID=Msa1038740-mRNA-1:exon:19402;Parent=Msa1038740-mRNA-1
chr7_1 exon 75718663 75718752 75718663 ID=Msa1038740-mRNA-1:exon:19403;Parent=Msa1038740-mRNA-1
chr7_1 exon 75718776 75718791 75718776 ID=Msa1038740-mRNA-1:exon:19404;Parent=Msa1038740-mRNA-1
chr7_1 exon 75718910 75719155 75718910 ID=Msa1038740-mRNA-1:exon:19405;Parent=Msa1038740-mRNA-1
chr7_1 five_prime_UTR 75716205 75716451 75716205 ID=Msa1038740-mRNA-1:five_prime_utr;Parent=Msa1038740-mRNA-1
chr7_1 CDS 75716452 75716603 75716452 ID=Msa1038740-mRNA-1:cds;Parent=Msa1038740-mRNA-1
chr7_1 CDS 75716940 75717057 75716940 ID=Msa1038740-mRNA-1:cds;Parent=Msa1038740-mRNA-1
chr7_1 CDS 75717147 75717378 75717147 ID=Msa1038740-mRNA-1:cds;Parent=Msa1038740-mRNA-1
chr7_1 CDS 75718663 75718691 75718663 ID=Msa1038740-mRNA-1:cds;Parent=Msa1038740-mRNA-1
chr7_1 three_prime_UTR 75718692 75718752 75718692 ID=Msa1038740-mRNA-1:three_prime_utr;Parent=Msa1038740-mRNA-1
chr7_1 three_prime_UTR 75718776 75718791 75718776 ID=Msa1038740-mRNA-1:three_prime_utr;Parent=Msa1038740-mRNA-1
chr7_1 three_prime_UTR 75718910 75719155 75718910 ID=Msa1038740-mRNA-1:three_prime_utr;Parent=Msa1038740-mRNA-1
Gene Sequence

>Msa1038740

ATGGTGAGGGAAGTTTCAGAGAGTTGCGTTGATAGCTTGATGACGGAGATGGTTGCTTGTTATTGCAATCGATTCTATGCCAACAAACCTGAACTTGCTGCTCGCAGGATCGAAGCCATTGGTTATCAGGTCGGTCAACAGCTCTCTGAAAGATATACGATGGAGCGGCCTCGGTTTGGTGATCATTTGGAGGCAATTAAGTTCATTTGCAAGGATTTTTGGTCTGAGGTGTTTAAGAAGCAGATAGACAACTTGAAAACTAACCATAGGGGTACATTTGTGTTGCAAGATAATAAATTTCCGTGGCTTGCACGGATGTCGGTTGATCCATCCACTGATAATGTTAGTTCAGTTGAGGATTATTCTGCACCTACGGCTGAAAGTAAGGCAGCACAGGCGATGAGCATGCATCTTTATTTCCCATGTGGGATCATTAGAGGAGCTCTTTCCAACTTGGGAATTCCCTGTGCAGTTTCTGCAGACATATCAAGCCTTCCAGCATGTTCATTTGTTGTCCGTATAAAAGCTTGA
Protein sequence

>Msa1038740

MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSTDNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSLPACSFVVRIKA*