AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0214550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0214550 MtrunA17_Chr2g0315551 99.539 217 1 0 42 258 1 217 4.40e-160 442
Msa0214550 MtrunA17_Chr2g0319951 82.110 218 38 1 42 258 1 218 6.16e-127 358
Msa0214550 MtrunA17_Chr2g0283131 78.539 219 43 3 42 258 1 217 5.99e-125 353
Msa0214550 MtrunA17_Chr4g0046261 78.082 219 44 3 42 258 1 217 2.11e-124 352
Msa0214550 MtrunA17_Chr4g0040781 76.712 219 47 3 42 258 1 217 9.26e-124 350
Msa0214550 MtrunA17_Chr2g0325091 75.472 212 49 3 48 258 5 214 2.53e-116 332
Msa0214550 MtrunA17_Chr4g0032201 68.224 214 67 1 45 258 3 215 2.28e-108 311
Msa0214550 MtrunA17_Chr3g0112681 65.116 215 75 0 44 258 2 216 3.00e-104 301
Msa0214550 MtrunA17_Chr2g0276941 66.516 221 69 2 39 258 4 220 7.29e-103 298
Msa0214550 MtrunA17_Chr8g0381841 59.211 228 74 4 43 258 5 225 9.03e-92 270
Msa0214550 MtrunA17_Chr1g0174281 61.137 211 77 3 52 257 13 223 1.68e-91 269
Msa0214550 MtrunA17_Chr7g0231131 59.420 207 82 1 52 256 13 219 4.21e-90 266
Msa0214550 MtrunA17_Chr3g0123821 59.174 218 85 4 43 256 4 221 8.76e-90 265
Msa0214550 MtrunA17_Chr6g0482721 57.619 210 89 0 48 257 6 215 2.42e-85 253
Msa0214550 MtrunA17_Chr8g0346421 53.017 232 101 3 31 257 3 231 2.67e-83 249
Msa0214550 MtrunA17_Chr7g0275991 52.889 225 99 2 40 257 3 227 2.90e-81 243
Msa0214550 MtrunA17_Chr7g0214241 65.116 172 60 0 53 224 14 185 2.06e-80 241
Msa0214550 MtrunA17_Chr8g0351861 52.423 227 97 4 42 257 1 227 6.99e-79 237
Msa0214550 MtrunA17_Chr1g0189361 53.774 212 92 2 52 257 12 223 2.27e-77 233
Msa0214550 MtrunA17_Chr8g0392931 55.140 214 89 2 48 257 8 218 7.77e-77 232
Msa0214550 MtrunA17_Chr7g0234201 61.628 172 66 0 53 224 12 183 1.09e-75 229
Msa0214550 MtrunA17_Chr2g0290891 46.009 213 105 2 51 257 3 211 3.53e-63 197
Msa0214550 MtrunA17_Chr6g0458081 53.939 165 76 0 51 215 3 167 1.07e-60 190
Msa0214550 MtrunA17_Chr4g0055651 45.972 211 104 2 48 258 2 202 7.11e-60 188
Msa0214550 MtrunA17_Chr8g0335471 47.619 210 99 3 50 258 4 203 8.27e-59 185
Msa0214550 MtrunA17_Chr7g0250531 46.411 209 102 2 50 258 4 202 2.51e-58 184
Msa0214550 MtrunA17_Chr8g0349711 45.024 211 107 3 53 257 5 212 1.07e-57 183
Msa0214550 MtrunA17_Chr1g0182321 43.925 214 115 2 46 258 7 216 1.08e-54 175
Msa0214550 MtrunA17_Chr1g0212521 44.231 208 111 2 50 256 11 214 1.97e-54 174
Msa0214550 MtrunA17_Chr1g0182301 43.458 214 116 2 46 258 7 216 4.38e-54 174
Msa0214550 MtrunA17_Chr4g0030911 45.070 213 101 5 50 256 11 213 1.24e-53 172
Msa0214550 MtrunA17_Chr7g0263071 50.898 167 81 1 50 215 11 177 9.28e-53 170
Msa0214550 MtrunA17_Chr2g0276931 41.905 210 113 2 47 256 2 202 1.24e-52 169
Msa0214550 MtrunA17_Chr8g0349681 45.894 207 109 3 51 257 381 584 1.43e-52 179
Msa0214550 MtrunA17_Chr8g0349681 50.641 156 77 0 53 208 12 167 1.39e-48 169
Msa0214550 MtrunA17_Chr8g0349681 46.108 167 90 0 51 217 172 338 2.22e-48 168
Msa0214550 MtrunA17_Chr1g0199331 47.239 163 85 1 56 217 33 195 1.01e-49 162
Msa0214550 MtrunA17_Chr3g0109721 39.806 206 103 3 56 257 12 200 6.25e-48 157
Msa0214550 MtrunA17_Chr2g0327911 45.087 173 93 2 45 215 6 178 1.95e-47 157
Msa0214550 MtrunA17_Chr5g0433561 39.806 206 103 3 56 257 12 200 6.74e-47 155
Msa0214550 MtrunA17_Chr8g0384311 41.148 209 112 5 50 256 9 208 4.85e-46 153
Msa0214550 MtrunA17_Chr7g0265001 44.498 209 107 4 51 257 13 214 2.08e-45 151
Msa0214550 MtrunA17_Chr1g0159481 45.833 168 88 3 50 215 13 179 3.07e-44 148
Msa0214550 MtrunA17_Chr1g0180291 43.269 208 108 5 51 256 11 210 4.49e-44 148
Msa0214550 MtrunA17_Chr1g0197331 41.589 214 113 5 47 257 8 212 6.18e-44 147
Msa0214550 MtrunA17_Chr8g0390561 41.250 160 94 0 56 215 11 170 5.73e-43 144
Msa0214550 MtrunA17_Chr3g0130271 42.056 214 104 9 50 256 8 208 1.69e-42 144
Msa0214550 MtrunA17_Chr5g0400131 37.580 157 98 0 55 211 10 166 1.75e-39 136
Msa0214550 MtrunA17_Chr4g0055081 36.943 157 99 0 55 211 10 166 9.77e-39 134
Msa0214550 MtrunA17_Chr3g0123971 35.669 157 101 0 55 211 10 166 1.52e-38 134
Msa0214550 MtrunA17_Chr8g0382221 35.669 157 101 0 55 211 10 166 2.04e-38 133
Msa0214550 MtrunA17_Chr6g0466301 38.788 165 94 3 54 211 8 172 1.73e-35 125
Msa0214550 MtrunA17_Chr6g0465491 38.788 165 94 3 54 211 8 172 1.73e-35 125
Msa0214550 MtrunA17_Chr8g0383261 34.848 198 112 3 54 234 12 209 5.34e-35 124
Msa0214550 MtrunA17_Chr4g0034871 33.971 209 119 7 54 255 8 204 2.29e-34 122
Msa0214550 MtrunA17_Chr2g0331691 33.482 224 120 6 39 255 6 207 2.71e-34 122
Msa0214550 MtrunA17_Chr4g0004661 33.036 224 121 6 39 255 60 261 8.77e-34 123
Msa0214550 MtrunA17_Chr1g0201121 38.889 162 92 4 54 208 8 169 1.81e-33 120
Msa0214550 MtrunA17_Chr6g0465471 32.143 196 109 6 69 255 5 185 7.86e-25 98.2
Msa0214550 MtrunA17_Chr6g0466161 35.570 149 85 3 69 208 5 151 3.27e-24 96.7
Msa0214550 MtrunA17_Chr3g0138121 32.468 154 101 2 55 208 14 164 4.71e-23 93.6
Msa0214550 MtrunA17_Chr3g0138111 32.468 154 101 2 55 208 14 164 5.07e-23 93.6
Msa0214550 MtrunA17_Chr3g0138101 32.468 154 101 2 55 208 14 164 5.23e-23 93.6
Msa0214550 MtrunA17_Chr3g0117931 30.387 181 112 5 54 222 8 186 6.09e-21 87.8
Msa0214550 MtrunA17_Chr4g0046211 37.500 120 72 3 54 172 6 123 2.44e-20 84.7
Msa0214550 MtrunA17_Chr4g0037021 38.333 120 71 3 54 172 6 123 1.43e-19 84.0
Msa0214550 MtrunA17_Chr2g0325101 36.364 121 74 3 54 173 6 124 5.70e-19 82.4
Msa0214550 MtrunA17_Chr6g0486041 36.364 121 74 3 54 173 6 124 6.63e-19 82.4
Msa0214550 MtrunA17_Chr5g0405791 35.833 120 74 3 54 172 6 123 1.44e-18 81.3
Msa0214550 MtrunA17_Chr8g0371501 35.833 120 74 3 54 172 6 123 4.57e-18 79.7
Msa0214550 MtrunA17_Chr6g0466431 34.343 99 56 1 54 152 8 97 1.55e-16 73.2
Msa0214550 MtrunA17_Chr6g0465611 34.343 99 56 1 54 152 8 97 1.55e-16 73.2
Msa0214550 MtrunA17_Chr1g0178291 32.683 205 120 7 25 223 398 590 1.68e-15 75.9
Msa0214550 MtrunA17_Chr5g0443631 27.749 191 125 5 36 218 87 272 2.46e-14 71.2
Msa0214550 MtrunA17_Chr1g0182951 60.465 43 17 0 163 205 1 43 3.96e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0214550 AT1G16920.1 88.018 217 25 1 42 258 1 216 3.35e-141 395
Msa0214550 AT5G45750.1 85.714 217 30 1 42 258 1 216 5.91e-137 384
Msa0214550 AT4G18800.1 83.410 217 33 2 42 258 1 214 1.08e-133 376
Msa0214550 AT1G06400.1 78.341 217 46 1 42 258 1 216 3.89e-127 359
Msa0214550 AT5G60860.1 76.256 219 48 3 42 258 1 217 3.43e-123 350
Msa0214550 AT3G15060.1 75.799 219 49 3 42 258 1 217 2.18e-122 347
Msa0214550 AT4G18430.1 72.936 218 55 2 42 257 1 216 5.81e-120 341
Msa0214550 AT1G28550.1 71.689 219 59 2 42 258 1 218 1.56e-119 340
Msa0214550 AT2G33870.1 71.233 219 60 2 42 258 1 218 5.65e-117 334
Msa0214550 AT1G09630.1 68.692 214 65 2 46 258 4 216 9.58e-107 308
Msa0214550 AT1G07410.1 68.545 213 65 2 46 258 4 214 1.76e-106 307
Msa0214550 AT5G59150.1 67.130 216 68 2 45 258 3 217 1.90e-101 295
Msa0214550 AT3G46830.1 67.130 216 68 2 45 258 3 217 1.36e-100 292
Msa0214550 AT3G46830.2 67.130 216 68 2 45 258 3 217 1.36e-100 292
Msa0214550 AT5G47960.1 63.810 210 73 1 52 258 13 222 7.09e-94 275
Msa0214550 AT3G12160.1 60.577 208 78 2 52 256 13 219 1.22e-91 270
Msa0214550 AT4G39990.1 60.185 216 70 3 52 258 15 223 3.56e-90 266
Msa0214550 AT5G65270.1 61.033 213 76 3 52 258 15 226 1.11e-88 263
Msa0214550 AT5G47520.1 56.757 222 90 4 42 258 1 221 1.14e-83 249
Msa0214550 AT2G43130.1 55.708 219 82 4 47 257 2 213 2.49e-80 241
Msa0214550 AT2G31680.1 54.839 217 80 3 52 258 10 218 2.69e-78 236
Msa0214550 AT1G18200.1 51.982 227 97 2 42 256 1 227 3.23e-77 233
Msa0214550 AT1G05810.1 55.814 215 80 4 52 258 10 217 3.60e-77 233
Msa0214550 AT3G07410.1 61.453 179 69 0 44 222 2 180 8.81e-77 232
Msa0214550 AT1G05810.2 54.955 222 83 5 47 258 49 263 8.83e-77 234
Msa0214550 AT1G73640.1 53.648 233 88 4 42 256 1 231 1.25e-76 232
Msa0214550 AT1G01200.1 54.460 213 88 4 52 257 26 236 7.86e-74 225
Msa0214550 AT5G59150.2 61.818 165 60 2 96 258 3 166 6.63e-67 205
Msa0214550 AT4G17170.1 46.009 213 105 2 51 257 3 211 5.04e-63 197
Msa0214550 AT4G17160.1 47.343 207 103 2 51 256 3 204 8.29e-62 193
Msa0214550 AT4G35860.1 53.939 165 76 0 51 215 3 167 1.30e-60 191
Msa0214550 AT1G02130.1 47.170 212 101 3 48 258 2 203 1.72e-59 187
Msa0214550 AT5G47200.1 45.972 211 104 2 48 258 2 202 3.46e-58 184
Msa0214550 AT4G17530.1 45.498 211 105 2 48 258 2 202 5.40e-58 184
Msa0214550 AT3G11730.1 43.750 208 110 1 49 256 3 203 7.43e-57 181
Msa0214550 AT5G03520.1 45.161 217 113 2 42 257 3 214 1.00e-54 176
Msa0214550 AT3G46060.2 52.500 160 75 1 50 208 11 170 2.00e-52 170
Msa0214550 AT3G46060.3 52.500 160 75 1 50 208 11 170 2.00e-52 170
Msa0214550 AT3G46060.1 52.500 160 75 1 50 208 11 170 2.00e-52 170
Msa0214550 AT3G09900.1 42.857 217 120 2 42 257 3 216 3.23e-52 169
Msa0214550 AT5G59840.1 50.299 167 82 1 50 215 11 177 5.96e-52 169
Msa0214550 AT5G45130.1 45.109 184 95 1 56 239 12 189 3.17e-51 166
Msa0214550 AT3G53610.2 49.701 167 83 1 50 215 11 177 3.67e-51 167
Msa0214550 AT3G53610.3 49.701 167 83 1 50 215 11 177 3.67e-51 167
Msa0214550 AT3G53610.1 49.701 167 83 1 50 215 11 177 3.67e-51 167
Msa0214550 AT4G19640.1 46.626 163 87 0 56 218 12 174 2.15e-50 164
Msa0214550 AT5G45130.2 45.055 182 94 1 58 239 22 197 2.57e-50 164
Msa0214550 AT1G43890.3 45.545 202 102 5 42 237 1 200 3.96e-50 164
Msa0214550 AT1G43890.1 45.545 202 102 5 42 237 1 200 3.96e-50 164
Msa0214550 AT1G43890.2 45.545 202 102 5 42 237 1 200 3.96e-50 164
Msa0214550 AT3G54840.1 47.853 163 84 1 56 217 35 197 5.51e-49 160
Msa0214550 AT3G54840.3 47.531 162 84 1 57 217 2 163 1.17e-48 159
Msa0214550 AT3G54840.2 49.351 154 77 1 56 208 35 188 1.54e-47 156
Msa0214550 AT5G03530.2 43.119 218 114 5 42 257 1 210 4.86e-46 153
Msa0214550 AT5G03530.1 43.119 218 114 5 42 257 1 210 4.86e-46 153
Msa0214550 AT4G35860.2 51.639 122 59 0 96 217 2 123 1.78e-41 140
Msa0214550 AT2G22290.1 36.306 157 100 0 55 211 10 166 3.17e-38 133
Msa0214550 AT5G03520.2 41.765 170 93 2 89 257 40 204 4.25e-38 132
Msa0214550 AT4G19640.3 46.565 131 70 0 56 186 12 142 1.37e-37 131
Msa0214550 AT5G10260.1 35.669 157 101 0 55 211 10 166 1.72e-37 131
Msa0214550 AT4G39890.1 36.709 158 99 1 55 211 10 167 2.94e-37 130
Msa0214550 AT4G19640.2 47.200 125 66 0 56 180 12 136 5.56e-37 128
Msa0214550 AT2G44610.1 36.306 157 100 0 55 211 10 166 6.98e-37 129
Msa0214550 AT1G22740.1 40.123 162 90 3 54 208 8 169 1.26e-36 129
Msa0214550 AT3G09910.3 41.414 198 108 4 42 237 1 192 1.76e-36 129
Msa0214550 AT3G09910.1 41.414 198 108 4 42 237 1 192 1.76e-36 129
Msa0214550 AT3G09910.2 41.414 198 108 4 42 237 1 192 1.76e-36 129
Msa0214550 AT5G64990.1 35.404 161 104 0 55 215 8 168 3.26e-36 128
Msa0214550 AT5G46025.1 58.491 106 43 1 116 220 6 111 4.87e-36 124
Msa0214550 AT3G09910.5 41.026 195 107 4 42 229 1 194 1.47e-35 126
Msa0214550 AT3G09910.4 41.026 195 107 4 42 229 1 194 1.47e-35 126
Msa0214550 AT2G21880.1 38.788 165 94 3 54 211 9 173 1.89e-34 123
Msa0214550 AT3G18820.1 35.450 189 107 4 54 235 8 188 1.96e-34 123
Msa0214550 AT1G52280.1 38.095 168 96 4 54 214 8 174 4.55e-34 122
Msa0214550 AT5G64990.2 33.929 168 104 1 55 215 8 175 1.52e-33 121
Msa0214550 AT3G16100.1 37.654 162 94 3 54 208 8 169 9.52e-33 119
Msa0214550 AT1G49300.2 36.527 167 99 3 54 213 8 174 1.82e-32 118
Msa0214550 AT1G49300.1 36.527 167 99 3 54 213 8 174 1.82e-32 118
Msa0214550 AT4G09720.1 36.420 162 96 2 54 208 8 169 6.01e-32 117
Msa0214550 AT5G39620.1 34.392 189 113 4 55 235 6 191 3.20e-31 115
Msa0214550 AT4G09720.4 37.126 167 93 4 54 208 8 174 1.30e-30 113
Msa0214550 AT4G09720.3 35.838 173 93 4 54 208 8 180 1.98e-29 110
Msa0214550 AT5G55080.1 35.256 156 94 3 55 208 14 164 5.89e-27 104
Msa0214550 AT2G21880.4 35.811 148 88 3 71 211 1 148 2.63e-25 99.4
Msa0214550 AT2G21880.3 35.811 148 88 3 71 211 1 148 2.63e-25 99.4
Msa0214550 AT2G21880.2 33.939 165 94 4 54 211 9 165 1.44e-24 97.8
Msa0214550 AT5G20020.1 32.468 154 101 2 55 208 14 164 7.00e-23 93.6
Msa0214550 AT5G55190.1 32.468 154 101 2 55 208 14 164 7.85e-23 93.6
Msa0214550 AT5G20010.1 32.468 154 101 2 55 208 14 164 7.85e-23 93.6
Msa0214550 AT2G44690.1 32.222 180 108 5 54 221 18 195 2.01e-21 89.4
Msa0214550 AT4G08190.1 63.636 66 21 1 143 208 65 127 2.99e-21 86.7
Msa0214550 AT4G09720.6 34.815 135 81 2 81 208 1 135 3.74e-21 87.8
Msa0214550 AT4G09720.5 34.815 135 81 2 81 208 1 135 3.74e-21 87.8
Msa0214550 AT4G09720.2 34.815 135 81 2 81 208 1 135 3.74e-21 87.8
Msa0214550 AT4G35020.1 34.132 167 96 5 54 208 6 170 2.64e-20 86.3
Msa0214550 AT4G35020.2 34.132 167 96 5 54 208 6 170 2.64e-20 86.3
Msa0214550 AT4G35020.3 34.132 167 96 5 54 208 6 170 2.64e-20 86.3
Msa0214550 AT4G28950.1 29.121 182 117 5 54 225 6 185 1.83e-19 84.3
Msa0214550 AT3G48040.1 29.949 197 120 6 54 238 8 198 5.20e-19 83.2
Msa0214550 AT1G75840.1 31.737 167 100 5 54 208 6 170 6.27e-19 82.4
Msa0214550 AT5G45970.1 37.500 120 72 3 54 172 6 123 9.48e-19 82.0
Msa0214550 AT1G20090.1 31.138 167 101 5 54 208 5 169 1.14e-18 81.6
Msa0214550 AT5G62880.1 29.545 176 110 5 54 217 8 181 7.14e-18 80.1
Msa0214550 AT3G51300.1 36.441 118 72 3 56 172 8 123 9.61e-18 79.3
Msa0214550 AT4G35950.1 35.833 120 74 3 54 172 6 123 9.71e-18 79.3
Msa0214550 AT2G17800.2 35.833 120 74 3 54 172 6 123 9.81e-18 79.3
Msa0214550 AT2G17800.1 35.833 120 74 3 54 172 6 123 9.81e-18 79.3
Msa0214550 AT4G35020.4 38.596 114 67 3 56 168 8 119 1.34e-17 77.8
Msa0214550 AT4G35020.5 38.596 114 67 3 56 168 8 119 1.34e-17 77.8
Msa0214550 AT3G48040.2 31.210 157 94 5 54 198 8 162 3.16e-17 77.4
Msa0214550 AT5G62880.2 35.000 120 75 3 54 172 8 125 6.44e-17 77.0

Find 57 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAACACCAATGGTGGATT+TGG 0.236269 2_1:-59070293 None:intergenic
TGTTGATTCTAAAGTTATTA+AGG 0.259169 2_1:+59070336 Msa0214550:CDS
TGGAAGTAATTAATTCATTA+AGG 0.261287 2_1:+59074022 Msa0214550:three_prime_UTR
GAAAGAATCACTTTATTTCA+TGG 0.263251 2_1:+59073551 Msa0214550:CDS
GATTGGAAACATAATTCATT+TGG 0.342285 2_1:+59073768 Msa0214550:three_prime_UTR
ATTTGTATCTCTCTCACTTT+CGG 0.348472 2_1:-59073822 None:intergenic
GACAAGTTTCATGTTTCAAT+AGG 0.358466 2_1:+59073910 Msa0214550:three_prime_UTR
GCTGTAGAGGGTGCTGAAAA+TGG 0.383578 2_1:+59073657 Msa0214550:CDS
GAAATAAAGTGATTCTTTCT+CGG 0.391175 2_1:-59073547 None:intergenic
ATTTGAAGACTTCCAGTAGT+TGG 0.393369 2_1:-59074098 None:intergenic
AAGTTATTAAGGCTCAGATC+TGG 0.406315 2_1:+59070347 Msa0214550:CDS
CATGTTTCAATAGGAGCTGC+TGG 0.408107 2_1:+59073919 Msa0214550:three_prime_UTR
GTTGCTGCTCAAGCTAAGAT+TGG 0.425537 2_1:+59073751 Msa0214550:exon
AACATTGTTGTGATGCTTGT+TGG 0.427166 2_1:+59073465 Msa0214550:CDS
TAATAGAATCATCCAACTAC+TGG 0.435340 2_1:+59074086 Msa0214550:three_prime_UTR
TTTCATGGAAACCTCAGCTC+TGG 0.442500 2_1:+59073566 Msa0214550:CDS
CGATCAGAACTACCTTGAAT+AGG 0.446974 2_1:-59070204 None:intergenic
GGTGAAACGAGAGAGAAGAT+TGG 0.454307 2_1:-59070248 None:intergenic
CGACTATGACTACCTATTCA+AGG 0.457570 2_1:+59070192 Msa0214550:CDS
ATAATTCATTTGGTTGCTAT+CGG 0.460679 2_1:+59073778 Msa0214550:three_prime_UTR
CAGCTTTCTTGCTAACAATA+CGG 0.494643 2_1:-59073638 None:intergenic
CTTCCCATCTTCAGTTGATA+CGG 0.496302 2_1:-59073517 None:intergenic
AAATGGAAACGCGTCTGTGC+CGG 0.506205 2_1:+59073674 Msa0214550:CDS
CTGATCGGCGACTCCGGCGT+TGG 0.508206 2_1:+59070220 Msa0214550:CDS
AACGCGTCTGTGCCGGCCAA+AGG 0.514171 2_1:+59073681 Msa0214550:CDS
GCTTACTATCGTGGGGCAGT+TGG 0.519994 2_1:+59073354 Msa0214550:CDS
TCTGCAAATTGATTCAAGCT+TGG 0.529602 2_1:+59074002 Msa0214550:three_prime_UTR
CTTCAGTTGATACGGCTACA+AGG 0.532280 2_1:-59073509 None:intergenic
GTAGTTCTGATCGGCGACTC+CGG 0.534376 2_1:+59070214 Msa0214550:CDS
AGTTATTAAGGCTCAGATCT+GGG 0.536509 2_1:+59070348 Msa0214550:CDS
GTTTCAGCTTTGAAGAGAGT+TGG 0.538571 2_1:+59073729 Msa0214550:CDS
AACAAGCATCACAACAATGT+TGG 0.538592 2_1:-59073463 None:intergenic
ATGTTTCAATAGGAGCTGCT+GGG 0.538879 2_1:+59073920 Msa0214550:three_prime_UTR
CTTGAATCCAAATCCACCAT+TGG 0.540714 2_1:+59070286 Msa0214550:CDS
GGGATACCGCTGGTCAAGAA+AGG 0.543539 2_1:+59070368 Msa0214550:CDS
ACGATAGTAAGCACTTGTAA+TGG 0.555075 2_1:-59073343 None:intergenic
GTAGCCGTATCAACTGAAGA+TGG 0.561123 2_1:+59073513 Msa0214550:CDS
ATTACAAGTGCTTACTATCG+TGG 0.574326 2_1:+59073345 Msa0214550:CDS
TTACAAGTGCTTACTATCGT+GGG 0.574573 2_1:+59073346 Msa0214550:CDS
CATGGAAACCTCAGCTCTGG+AGG 0.574684 2_1:+59073569 Msa0214550:CDS
AGCGAATTCAACACCAATGG+TGG 0.577629 2_1:-59070299 None:intergenic
CTATTCAAGGTAGTTCTGAT+CGG 0.585029 2_1:+59070205 Msa0214550:CDS
TGTTAGCAAGAAAGCTGTAG+AGG 0.588403 2_1:+59073644 Msa0214550:CDS
CGACGTTCTCAAATGTTGCA+TGG 0.590652 2_1:-59073404 None:intergenic
TAGCCGTATCAACTGAAGAT+GGG 0.593299 2_1:+59073514 Msa0214550:CDS
GCTCAGATCTGGGATACCGC+TGG 0.595276 2_1:+59070358 Msa0214550:CDS
GTTAGCAAGAAAGCTGTAGA+GGG 0.598414 2_1:+59073645 Msa0214550:CDS
AAGATTGGATTTGCCAACGC+CGG 0.601803 2_1:-59070233 None:intergenic
CATTTGAGAACGTCGACAGA+TGG 0.604678 2_1:+59073412 Msa0214550:CDS
TGTTTCAATAGGAGCTGCTG+GGG 0.613226 2_1:+59073921 Msa0214550:three_prime_UTR
GTTAGTAGCCTCCAGAGCTG+AGG 0.627901 2_1:-59073577 None:intergenic
AAAAGACCTTTCTTGACCAG+CGG 0.653527 2_1:-59070374 None:intergenic
TTGATACGGCTACAAGGTGA+CGG 0.654516 2_1:-59073503 None:intergenic
TACAAGTGCTTACTATCGTG+GGG 0.673719 2_1:+59073347 Msa0214550:CDS
AGTAAGCACTTGTAATGGCG+CGG 0.690463 2_1:-59073338 None:intergenic
AGTAGCGAATTCAACACCAA+TGG 0.690637 2_1:-59070302 None:intergenic
CAACAATGTTGGAATCTGTG+TGG 0.736098 2_1:-59073452 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTAATAATTCTATTAG+CGG + chr2_1:59070724-59070743 Msa0214550:intron 10.0%
!!! AATTTTTATTTTTTTTGCTA+AGG - chr2_1:59071047-59071066 None:intergenic 10.0%
!!! TCTAAAATATGATTTAATTT+TGG + chr2_1:59071071-59071090 Msa0214550:intron 10.0%
!! AAGAATTTATATTACAAAAT+TGG + chr2_1:59071827-59071846 Msa0214550:intron 10.0%
!!! TTGTTTTATATATTCTATGA+TGG + chr2_1:59071443-59071462 Msa0214550:intron 15.0%
!! AATATAAATTCTTAAGAGTT+GGG - chr2_1:59071820-59071839 None:intergenic 15.0%
!! TAATATAAATTCTTAAGAGT+TGG - chr2_1:59071821-59071840 None:intergenic 15.0%
!! CTATTCAAATATTTGAAAAT+AGG - chr2_1:59071874-59071893 None:intergenic 15.0%
!!! TGTTGATTCTAAAGTTATTA+AGG + chr2_1:59070336-59070355 Msa0214550:CDS 20.0%
!!! TCAGAGATCTTTAATTTAAA+TGG + chr2_1:59070620-59070639 Msa0214550:intron 20.0%
!!! ATGAAGAAGAAATGAATTTT+AGG + chr2_1:59070698-59070717 Msa0214550:intron 20.0%
!! TTTGTTTAAAAAAGATCAAG+AGG + chr2_1:59070880-59070899 Msa0214550:intron 20.0%
!!! TTTTTGCTAAGGAAAATTTA+GGG - chr2_1:59071036-59071055 None:intergenic 20.0%
!!! TTTTTTGCTAAGGAAAATTT+AGG - chr2_1:59071037-59071056 None:intergenic 20.0%
!! AAGGAATTACAAAAAACTAA+AGG + chr2_1:59071123-59071142 Msa0214550:intron 20.0%
!! AGGAATTACAAAAAACTAAA+GGG + chr2_1:59071124-59071143 Msa0214550:intron 20.0%
!! TAATAATAACTAAAGCCTAA+AGG - chr2_1:59071252-59071271 None:intergenic 20.0%
!!! TATACTTCAATTTTTTTGAC+TGG + chr2_1:59071280-59071299 Msa0214550:intron 20.0%
!!! ATATCTTATTTAAGAACTGT+TGG + chr2_1:59071466-59071485 Msa0214550:intron 20.0%
!!! TATCTTATTTAAGAACTGTT+GGG + chr2_1:59071467-59071486 Msa0214550:intron 20.0%
!! AATTTATACTAAATAGCGTA+TGG + chr2_1:59071702-59071721 Msa0214550:intron 20.0%
!!! AGAAATGTTTTTATTTTCAC+TGG - chr2_1:59071987-59072006 None:intergenic 20.0%
!!! TTTTGAAAGAAAAAAGTTGA+TGG - chr2_1:59072414-59072433 None:intergenic 20.0%
!! TGATTAAAAAACCAGAATTA+TGG + chr2_1:59072726-59072745 Msa0214550:intron 20.0%
!! GATTAAAAAACCAGAATTAT+GGG + chr2_1:59072727-59072746 Msa0214550:intron 20.0%
!! ATTAAAAAACCAGAATTATG+GGG + chr2_1:59072728-59072747 Msa0214550:intron 20.0%
!!! AGATTTTTACTACTGATAAT+TGG - chr2_1:59072781-59072800 None:intergenic 20.0%
!! TTATCAGTAGTAAAAATCTA+AGG + chr2_1:59072782-59072801 Msa0214550:intron 20.0%
!!! AGTTCTAATACTAATAATAG+GGG - chr2_1:59072836-59072855 None:intergenic 20.0%
!!! GAGTTCTAATACTAATAATA+GGG - chr2_1:59072837-59072856 None:intergenic 20.0%
!!! AGAGTTCTAATACTAATAAT+AGG - chr2_1:59072838-59072857 None:intergenic 20.0%
!!! AAGTTCTAATACTAACAATT+GGG - chr2_1:59072898-59072917 None:intergenic 20.0%
!!! AAAGTTCTAATACTAACAAT+TGG - chr2_1:59072899-59072918 None:intergenic 20.0%
!! TTGTTAGTATTAGAACTTTA+AGG + chr2_1:59072900-59072919 Msa0214550:intron 20.0%
!! AAATAAAAACAATGTTACTC+AGG - chr2_1:59072954-59072973 None:intergenic 20.0%
!!! GTAACATTGTTTTTATTTAG+CGG + chr2_1:59072956-59072975 Msa0214550:intron 20.0%
!! TTATCAAACTTACAAATGAT+AGG + chr2_1:59072996-59073015 Msa0214550:intron 20.0%
!! TGGAAGTAATTAATTCATTA+AGG + chr2_1:59074022-59074041 Msa0214550:three_prime_UTR 20.0%
!! CATTTCTACATTTTGCTTAA+TGG + chr2_1:59070423-59070442 Msa0214550:intron 25.0%
! ATTTAAATGGTTAAGTGATG+TGG + chr2_1:59070633-59070652 Msa0214550:intron 25.0%
!! TAAGGAAAATTTAGGGTAAA+GGG - chr2_1:59071029-59071048 None:intergenic 25.0%
!! TGGCAAATACCAATTATTTT+AGG + chr2_1:59071091-59071110 Msa0214550:intron 25.0%
!!! GGCAAATACCAATTATTTTA+GGG + chr2_1:59071092-59071111 Msa0214550:intron 25.0%
!! TAGCTGCTATTTAACAATAT+TGG + chr2_1:59071781-59071800 Msa0214550:intron 25.0%
!! TTCTCACATTTTCATATAGA+AGG + chr2_1:59071914-59071933 Msa0214550:intron 25.0%
! ATGGTTAATGATATTGATGT+GGG + chr2_1:59072228-59072247 Msa0214550:intron 25.0%
! AATATGTAATGAAAGGAACA+TGG - chr2_1:59072306-59072325 None:intergenic 25.0%
! ACACTAGTGTAGTAATATAT+TGG + chr2_1:59072372-59072391 Msa0214550:intron 25.0%
! GAAGATAATGTAAACTTTGT+TGG - chr2_1:59072613-59072632 None:intergenic 25.0%
! TATTGTTCAATTACTCTTCA+AGG - chr2_1:59072654-59072673 None:intergenic 25.0%
!!! TAACTTGTATTTTTGAGCAA+AGG + chr2_1:59072702-59072721 Msa0214550:intron 25.0%
!! AGTTCTAATACTAACAATTG+GGG - chr2_1:59072897-59072916 None:intergenic 25.0%
! AAACTTACAAATGATAGGTA+TGG + chr2_1:59073001-59073020 Msa0214550:intron 25.0%
! ATCTGTAATCAAACTTACAA+AGG + chr2_1:59073085-59073104 Msa0214550:intron 25.0%
! TAATCAAACTTACAAAGGAT+AGG + chr2_1:59073090-59073109 Msa0214550:intron 25.0%
! TTACAAATCAATCGTAACAA+TGG - chr2_1:59073243-59073262 None:intergenic 25.0%
! GAAATAAAGTGATTCTTTCT+CGG - chr2_1:59073550-59073569 None:intergenic 25.0%
!! GAAAGAATCACTTTATTTCA+TGG + chr2_1:59073551-59073570 Msa0214550:CDS 25.0%
! GATTGGAAACATAATTCATT+TGG + chr2_1:59073768-59073787 Msa0214550:three_prime_UTR 25.0%
! ATAATTCATTTGGTTGCTAT+CGG + chr2_1:59073778-59073797 Msa0214550:three_prime_UTR 25.0%
!!! ACTTGTCTTTTAAGATTACA+TGG - chr2_1:59073897-59073916 None:intergenic 25.0%
!! TTTATTTGTGCACTATTTTG+TGG + chr2_1:59074047-59074066 Msa0214550:three_prime_UTR 25.0%
AAGAAGAAGAAGAAGAAGAA+TGG + chr2_1:59070147-59070166 Msa0214550:CDS 30.0%
! TTCAAGATTGAACTCGTTTT+TGG - chr2_1:59070272-59070291 None:intergenic 30.0%
! CTAAGGAAAATTTAGGGTAA+AGG - chr2_1:59071030-59071049 None:intergenic 30.0%
!!! TACCAATTATTTTAGGGCAT+TGG + chr2_1:59071098-59071117 Msa0214550:intron 30.0%
AACCAATGCCCTAAAATAAT+TGG - chr2_1:59071103-59071122 None:intergenic 30.0%
!!! TTATTTTAGGGCATTGGTTA+AGG + chr2_1:59071104-59071123 Msa0214550:intron 30.0%
! TTTCTAATTCTTGTTTGACC+TGG + chr2_1:59071215-59071234 Msa0214550:intron 30.0%
! TATGTTTAGAGTCCCATATT+TGG + chr2_1:59071511-59071530 Msa0214550:intron 30.0%
ACCTTCTTTCAACCAAATAT+GGG - chr2_1:59071526-59071545 None:intergenic 30.0%
!!! CTTACAAGCTAGTTTTGTAA+GGG + chr2_1:59071580-59071599 Msa0214550:intron 30.0%
AATAGCTGTTTGTCCAAATT+TGG + chr2_1:59071731-59071750 Msa0214550:intron 30.0%
! CATTTTCATATAGAAGGCTA+TGG + chr2_1:59071920-59071939 Msa0214550:intron 30.0%
AAGAAGAAACAGCGAAAATA+AGG + chr2_1:59071944-59071963 Msa0214550:intron 30.0%
ATCACACTCTTTCGATATTT+AGG + chr2_1:59072052-59072071 Msa0214550:intron 30.0%
TCACACTCTTTCGATATTTA+GGG + chr2_1:59072053-59072072 Msa0214550:intron 30.0%
!! AGTTTTCCATTTGGAATAGA+GGG - chr2_1:59072193-59072212 None:intergenic 30.0%
!! TAGTTTTCCATTTGGAATAG+AGG - chr2_1:59072194-59072213 None:intergenic 30.0%
! CAAAAGAGTAGTTTTCCATT+TGG - chr2_1:59072202-59072221 None:intergenic 30.0%
GATGGTTAATGATATTGATG+TGG + chr2_1:59072227-59072246 Msa0214550:intron 30.0%
TGATATTGATGTGGGTTTAT+AGG + chr2_1:59072236-59072255 Msa0214550:intron 30.0%
GGAACATGGAAAATTATAAC+TGG - chr2_1:59072292-59072311 None:intergenic 30.0%
TGGGATGAATATGTAATGAA+AGG - chr2_1:59072313-59072332 None:intergenic 30.0%
! ATGGGGATATTTGTTTCTTT+CGG - chr2_1:59072499-59072518 None:intergenic 30.0%
GTAGTTAATTCTTACTAGCA+GGG - chr2_1:59072531-59072550 None:intergenic 30.0%
AGTAGTTAATTCTTACTAGC+AGG - chr2_1:59072532-59072551 None:intergenic 30.0%
AAACTTACAAAGGATAGGTA+TGG + chr2_1:59073095-59073114 Msa0214550:intron 30.0%
CTAAAGTCAGTTGCAAAATT+TGG - chr2_1:59073273-59073292 None:intergenic 30.0%
! AAGATCTATTTTCTCTCCTT+TGG - chr2_1:59073700-59073719 None:intergenic 30.0%
ATTTGTATCTCTCTCACTTT+CGG - chr2_1:59073825-59073844 None:intergenic 30.0%
GACAAGTTTCATGTTTCAAT+AGG + chr2_1:59073910-59073929 Msa0214550:three_prime_UTR 30.0%
TAATAGAATCATCCAACTAC+TGG + chr2_1:59074086-59074105 Msa0214550:three_prime_UTR 30.0%
AAGAAGAAGAAGAAGAATGG+CGG + chr2_1:59070153-59070172 Msa0214550:CDS 35.0%
!! CTATTCAAGGTAGTTCTGAT+CGG + chr2_1:59070205-59070224 Msa0214550:CDS 35.0%
AAGTTATTAAGGCTCAGATC+TGG + chr2_1:59070347-59070366 Msa0214550:CDS 35.0%
AGTTATTAAGGCTCAGATCT+GGG + chr2_1:59070348-59070367 Msa0214550:CDS 35.0%
ACATCAGCACTACAAACAAA+TGG - chr2_1:59070579-59070598 None:intergenic 35.0%
! GAATTCAGTGTTGTGTCTTT+TGG + chr2_1:59070751-59070770 Msa0214550:intron 35.0%
TCCATAGTAGCAGTTGAATT+TGG + chr2_1:59070800-59070819 Msa0214550:intron 35.0%
TCCAAATTCAACTGCTACTA+TGG - chr2_1:59070804-59070823 None:intergenic 35.0%
TAGTAGCAGTTGAATTTGGA+CGG + chr2_1:59070804-59070823 Msa0214550:intron 35.0%
TTTACCATAGATCGAGTCTT+TGG - chr2_1:59070951-59070970 None:intergenic 35.0%
TCGATCTATGGTAAAAAGAC+AGG + chr2_1:59070956-59070975 Msa0214550:intron 35.0%
! GAAAATTTAGGGTAAAGGGA+TGG - chr2_1:59071025-59071044 None:intergenic 35.0%
GTCTAATCTTCAGTTGAACT+TGG + chr2_1:59071310-59071329 Msa0214550:intron 35.0%
ATGAGGCTTATGCATGAAAT+AGG - chr2_1:59071391-59071410 None:intergenic 35.0%
AACCAAGCAAGTTCAAAATG+AGG - chr2_1:59071408-59071427 None:intergenic 35.0%
TCCCATATTTGGTTGAAAGA+AGG + chr2_1:59071522-59071541 Msa0214550:intron 35.0%
GACCTTCTTTCAACCAAATA+TGG - chr2_1:59071527-59071546 None:intergenic 35.0%
TATTTGGTTGAAAGAAGGTC+TGG + chr2_1:59071527-59071546 Msa0214550:intron 35.0%
TGGATATGAGCTCATTAGTT+GGG + chr2_1:59071547-59071566 Msa0214550:intron 35.0%
! CCTTACAAAACTAGCTTGTA+AGG - chr2_1:59071582-59071601 None:intergenic 35.0%
!!! CCTTACAAGCTAGTTTTGTA+AGG + chr2_1:59071579-59071598 Msa0214550:intron 35.0%
! AACCAAGAGCATGTTTGATT+TGG - chr2_1:59072010-59072029 None:intergenic 35.0%
!! GTTTTCCATTTGGAATAGAG+GGG - chr2_1:59072192-59072211 None:intergenic 35.0%
! AACTACTCTTTTGCATGTGA+TGG + chr2_1:59072209-59072228 Msa0214550:intron 35.0%
ATTGGGTGCAGTGTTTAAAT+GGG - chr2_1:59072332-59072351 None:intergenic 35.0%
! ATATTTGTTTCTTTCGGGGA+GGG - chr2_1:59072493-59072512 None:intergenic 35.0%
! TGGGGATATTTGTTTCTTTC+GGG - chr2_1:59072498-59072517 None:intergenic 35.0%
ACATTATCTTCCCCTGTTAT+CGG + chr2_1:59072622-59072641 Msa0214550:intron 35.0%
AAAAAACCAGAATTATGGGG+TGG + chr2_1:59072731-59072750 Msa0214550:intron 35.0%
AAAAACCAGAATTATGGGGT+GGG + chr2_1:59072732-59072751 Msa0214550:intron 35.0%
ACTTATTGTACTTTAGGCCT+TGG - chr2_1:59073054-59073073 None:intergenic 35.0%
AAGGCCTAAAGTACAATAAG+TGG + chr2_1:59073053-59073072 Msa0214550:intron 35.0%
GACACCACTTATTGTACTTT+AGG - chr2_1:59073060-59073079 None:intergenic 35.0%
ACTTATTGTACTTTAGGCCT+TGG - chr2_1:59073054-59073073 None:intergenic 35.0%
AAGGCCTAAAGTACAATAAG+TGG + chr2_1:59073053-59073072 Msa0214550:intron 35.0%
GACACCACTTATTGTACTTT+AGG - chr2_1:59073060-59073079 None:intergenic 35.0%
ACGATAGTAAGCACTTGTAA+TGG - chr2_1:59073346-59073365 None:intergenic 35.0%
ATTACAAGTGCTTACTATCG+TGG + chr2_1:59073345-59073364 Msa0214550:CDS 35.0%
TTACAAGTGCTTACTATCGT+GGG + chr2_1:59073346-59073365 Msa0214550:CDS 35.0%
AACAAGCATCACAACAATGT+TGG - chr2_1:59073466-59073485 None:intergenic 35.0%
AACATTGTTGTGATGCTTGT+TGG + chr2_1:59073465-59073484 Msa0214550:CDS 35.0%
CAGCTTTCTTGCTAACAATA+CGG - chr2_1:59073641-59073660 None:intergenic 35.0%
! TCTGCAAATTGATTCAAGCT+TGG + chr2_1:59074002-59074021 Msa0214550:three_prime_UTR 35.0%
AGAAGAAGAAGAAGAATGGC+GGG + chr2_1:59070154-59070173 Msa0214550:CDS 40.0%
CGACTATGACTACCTATTCA+AGG + chr2_1:59070192-59070211 Msa0214550:CDS 40.0%
CGATCAGAACTACCTTGAAT+AGG - chr2_1:59070207-59070226 None:intergenic 40.0%
CTTGAATCCAAATCCACCAT+TGG + chr2_1:59070286-59070305 Msa0214550:CDS 40.0%
!! TTCAACACCAATGGTGGATT+TGG - chr2_1:59070296-59070315 None:intergenic 40.0%
AGTAGCGAATTCAACACCAA+TGG - chr2_1:59070305-59070324 None:intergenic 40.0%
AAAAGACCTTTCTTGACCAG+CGG - chr2_1:59070377-59070396 None:intergenic 40.0%
TAACAACAGATCACGATCGA+CGG - chr2_1:59070457-59070476 None:intergenic 40.0%
!! TAAACTTAGTAGCACCGACA+CGG + chr2_1:59070486-59070505 Msa0214550:intron 40.0%
ATCTCCAAAGACTCGATCTA+TGG + chr2_1:59070944-59070963 Msa0214550:intron 40.0%
AGGCAGAGAAGACAATCTAT+CGG - chr2_1:59070990-59071009 None:intergenic 40.0%
ATAGATTGTCTTCTCTGCCT+TGG + chr2_1:59070990-59071009 Msa0214550:intron 40.0%
! TTCTTGTTTGACCTGGTTAG+CGG + chr2_1:59071222-59071241 Msa0214550:intron 40.0%
!! AGCCTCATTTTGAACTTGCT+TGG + chr2_1:59071403-59071422 Msa0214550:intron 40.0%
TAGACCTCTCCAATATCAAG+CGG - chr2_1:59071493-59071512 None:intergenic 40.0%
CTGGATATGAGCTCATTAGT+TGG + chr2_1:59071546-59071565 Msa0214550:intron 40.0%
CTCTGTAGAGTAGCCAAATT+TGG - chr2_1:59071747-59071766 None:intergenic 40.0%
CAAATTTGGCTACTCTACAG+AGG + chr2_1:59071745-59071764 Msa0214550:intron 40.0%
CTCCAAATCAAACATGCTCT+TGG + chr2_1:59072005-59072024 Msa0214550:intron 40.0%
TGAGTAAAGTTGCCTATGGT+AGG + chr2_1:59072133-59072152 Msa0214550:intron 40.0%
!! AGCATTGTGTCGTTTGAGTA+GGG + chr2_1:59072165-59072184 Msa0214550:intron 40.0%
CATTGGGTGCAGTGTTTAAA+TGG - chr2_1:59072333-59072352 None:intergenic 40.0%
CTCTTCTACCATCTTGCATT+GGG - chr2_1:59072349-59072368 None:intergenic 40.0%
ACTCTTCTACCATCTTGCAT+TGG - chr2_1:59072350-59072369 None:intergenic 40.0%
! GATATTTGTTTCTTTCGGGG+AGG - chr2_1:59072494-59072513 None:intergenic 40.0%
! GGGGATATTTGTTTCTTTCG+GGG - chr2_1:59072497-59072516 None:intergenic 40.0%
! TGATTCAAGTTCTGTAGCCA+AGG + chr2_1:59073034-59073053 Msa0214550:intron 40.0%
! TGATTCAAGTTCTGTAGCCA+AGG + chr2_1:59073034-59073053 Msa0214550:intron 40.0%
! GATGCTGCATAGTTGCTTTA+TGG + chr2_1:59073216-59073235 Msa0214550:intron 40.0%
!! TTCACACTGTTTCTATCTGC+AGG + chr2_1:59073313-59073332 Msa0214550:intron 40.0%
TACAAGTGCTTACTATCGTG+GGG + chr2_1:59073347-59073366 Msa0214550:CDS 40.0%
CAACAATGTTGGAATCTGTG+TGG - chr2_1:59073455-59073474 None:intergenic 40.0%
TAGCCGTATCAACTGAAGAT+GGG + chr2_1:59073514-59073533 Msa0214550:CDS 40.0%
! CTTCCCATCTTCAGTTGATA+CGG - chr2_1:59073520-59073539 None:intergenic 40.0%
TGTTAGCAAGAAAGCTGTAG+AGG + chr2_1:59073644-59073663 Msa0214550:CDS 40.0%
GTTAGCAAGAAAGCTGTAGA+GGG + chr2_1:59073645-59073664 Msa0214550:CDS 40.0%
! TCTATTTTCTCTCCTTTGGC+CGG - chr2_1:59073696-59073715 None:intergenic 40.0%
!! GTTTCAGCTTTGAAGAGAGT+TGG + chr2_1:59073729-59073748 Msa0214550:CDS 40.0%
ATGTTTCAATAGGAGCTGCT+GGG + chr2_1:59073920-59073939 Msa0214550:three_prime_UTR 40.0%
AAGATTGGATTTGCCAACGC+CGG - chr2_1:59070236-59070255 None:intergenic 45.0%
GGTGAAACGAGAGAGAAGAT+TGG - chr2_1:59070251-59070270 None:intergenic 45.0%
AGCGAATTCAACACCAATGG+TGG - chr2_1:59070302-59070321 None:intergenic 45.0%
AACAACAGATCACGATCGAC+GGG - chr2_1:59070456-59070475 None:intergenic 45.0%
GTGTGACATCTATCAGACAC+CGG - chr2_1:59070521-59070540 None:intergenic 45.0%
AGCGTGAGTAAAGTTGCCTA+TGG + chr2_1:59072129-59072148 Msa0214550:intron 45.0%
TAAAGTTGCCTATGGTAGGC+TGG + chr2_1:59072137-59072156 Msa0214550:intron 45.0%
AAAGTTGCCTATGGTAGGCT+GGG + chr2_1:59072138-59072157 Msa0214550:intron 45.0%
!! GAGCATTGTGTCGTTTGAGT+AGG + chr2_1:59072164-59072183 Msa0214550:intron 45.0%
AAGGAACATGCCGATAACAG+GGG - chr2_1:59072635-59072654 None:intergenic 45.0%
CAAGGAACATGCCGATAACA+GGG - chr2_1:59072636-59072655 None:intergenic 45.0%
TCAAGGAACATGCCGATAAC+AGG - chr2_1:59072637-59072656 None:intergenic 45.0%
! TGGCACATCAGATTTTGCAC+AGG - chr2_1:59072761-59072780 None:intergenic 45.0%
AACAATTGGGGCACATCAAG+AGG - chr2_1:59072885-59072904 None:intergenic 45.0%
ATGCAGCATCGTGTTCATTC+AGG - chr2_1:59073206-59073225 None:intergenic 45.0%
AGTAAGCACTTGTAATGGCG+CGG - chr2_1:59073341-59073360 None:intergenic 45.0%
CGACGTTCTCAAATGTTGCA+TGG - chr2_1:59073407-59073426 None:intergenic 45.0%
CATTTGAGAACGTCGACAGA+TGG + chr2_1:59073412-59073431 Msa0214550:CDS 45.0%
TTGATACGGCTACAAGGTGA+CGG - chr2_1:59073506-59073525 None:intergenic 45.0%
! CTTCAGTTGATACGGCTACA+AGG - chr2_1:59073512-59073531 None:intergenic 45.0%
GTAGCCGTATCAACTGAAGA+TGG + chr2_1:59073513-59073532 Msa0214550:CDS 45.0%
TTTCATGGAAACCTCAGCTC+TGG + chr2_1:59073566-59073585 Msa0214550:CDS 45.0%
GTTGCTGCTCAAGCTAAGAT+TGG + chr2_1:59073751-59073770 Msa0214550:exon 45.0%
CATGTTTCAATAGGAGCTGC+TGG + chr2_1:59073919-59073938 Msa0214550:three_prime_UTR 45.0%
TGTTTCAATAGGAGCTGCTG+GGG + chr2_1:59073921-59073940 Msa0214550:three_prime_UTR 45.0%
! TGTGCACTATTTTGTGGTCG+TGG + chr2_1:59074053-59074072 Msa0214550:three_prime_UTR 45.0%
TTGTCTTCTCTGCCTTGGAC+TGG + chr2_1:59070995-59071014 Msa0214550:intron 50.0%
TGTCTTCTCTGCCTTGGACT+GGG + chr2_1:59070996-59071015 Msa0214550:intron 50.0%
CTAAAGGCTTACCGCTAACC+AGG - chr2_1:59071236-59071255 None:intergenic 50.0%
CTGGTTAGCGGTAAGCCTTT+AGG + chr2_1:59071234-59071253 Msa0214550:intron 50.0%
ACTGTTGGGCCGCTTGATAT+TGG + chr2_1:59071481-59071500 Msa0214550:intron 50.0%
AAGTTGCCTATGGTAGGCTG+GGG + chr2_1:59072139-59072158 Msa0214550:intron 50.0%
AGGGTCCCCTCTATTCCAAA+TGG + chr2_1:59072184-59072203 Msa0214550:intron 50.0%
ACACTGCACCCAATGCAAGA+TGG + chr2_1:59072338-59072357 Msa0214550:intron 50.0%
GGTATCCCACCCCATAATTC+TGG - chr2_1:59072740-59072759 None:intergenic 50.0%
CAGCATCGTGTTCATTCAGG+TGG - chr2_1:59073203-59073222 None:intergenic 50.0%
!! GCTGTAGAGGGTGCTGAAAA+TGG + chr2_1:59073657-59073676 Msa0214550:CDS 50.0%
AAATGGAAACGCGTCTGTGC+CGG + chr2_1:59073674-59073693 Msa0214550:CDS 50.0%
!! GTAGTTCTGATCGGCGACTC+CGG + chr2_1:59070214-59070233 Msa0214550:CDS 55.0%
GGGATACCGCTGGTCAAGAA+AGG + chr2_1:59070368-59070387 Msa0214550:CDS 55.0%
GTCTTCTCTGCCTTGGACTG+GGG + chr2_1:59070997-59071016 Msa0214550:intron 55.0%
TGGGCCGCTTGATATTGGAG+AGG + chr2_1:59071486-59071505 Msa0214550:intron 55.0%
!! TAGCAGGGTGCTGCTAGATG+GGG - chr2_1:59072516-59072535 None:intergenic 55.0%
!! CTAGCAGGGTGCTGCTAGAT+GGG - chr2_1:59072517-59072536 None:intergenic 55.0%
!! ACTAGCAGGGTGCTGCTAGA+TGG - chr2_1:59072518-59072537 None:intergenic 55.0%
GCTTACTATCGTGGGGCAGT+TGG + chr2_1:59073354-59073373 Msa0214550:CDS 55.0%
! CATGGAAACCTCAGCTCTGG+AGG + chr2_1:59073569-59073588 Msa0214550:CDS 55.0%
GTTAGTAGCCTCCAGAGCTG+AGG - chr2_1:59073580-59073599 None:intergenic 55.0%
! GCTCAGATCTGGGATACCGC+TGG + chr2_1:59070358-59070377 Msa0214550:CDS 60.0%
TTGCCTATGGTAGGCTGGGG+TGG + chr2_1:59072142-59072161 Msa0214550:intron 60.0%
ACCGACACGGAAGACGCGTC+CGG + chr2_1:59070499-59070518 Msa0214550:intron 65.0%
ACCGGACGCGTCTTCCGTGT+CGG - chr2_1:59070503-59070522 None:intergenic 65.0%
AGGGATGGTGCCCCAGTCCA+AGG - chr2_1:59071010-59071029 None:intergenic 65.0%
GCTCCACCCCAGCCTACCAT+AGG - chr2_1:59072148-59072167 None:intergenic 65.0%
! AACGCGTCTGTGCCGGCCAA+AGG + chr2_1:59073681-59073700 Msa0214550:CDS 65.0%
CTGATCGGCGACTCCGGCGT+TGG + chr2_1:59070220-59070239 Msa0214550:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 59070046 59074109 59070046 ID=Msa0214550;Name=Msa0214550
chr2_1 mRNA 59070046 59074109 59070046 ID=Msa0214550-mRNA-1;Parent=Msa0214550;Name=Msa0214550-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|1|1|1|1|1|2|342|258
chr2_1 exon 59070046 59070389 59070046 ID=Msa0214550-mRNA-1:exon:16233;Parent=Msa0214550-mRNA-1
chr2_1 exon 59073335 59074109 59073335 ID=Msa0214550-mRNA-1:exon:16234;Parent=Msa0214550-mRNA-1
chr2_1 CDS 59070046 59070389 59070046 ID=Msa0214550-mRNA-1:cds;Parent=Msa0214550-mRNA-1
chr2_1 CDS 59073335 59073767 59073335 ID=Msa0214550-mRNA-1:cds;Parent=Msa0214550-mRNA-1
chr2_1 three_prime_UTR 59073768 59074109 59073768 ID=Msa0214550-mRNA-1:three_prime_utr;Parent=Msa0214550-mRNA-1
Gene Sequence

>Msa0214550

GGCGCTTTTTTCATATTTCAACTCAACACTACTCTCACTTTTTCTTTTTCTCTGCACATCTCTCTCTTGTTTCTTCTCATAGCAACAGAGCAGATTCATCAAAGAAGAAGAAGAAGAAGAAGAATGGCGGGTTACAGAGCAGACGACGACTATGACTACCTATTCAAGGTAGTTCTGATCGGCGACTCCGGCGTTGGCAAATCCAATCTTCTCTCTCGTTTCACCAAAAACGAGTTCAATCTTGAATCCAAATCCACCATTGGTGTTGAATTCGCTACTCGTACTTTAAATGTTGATTCTAAAGTTATTAAGGCTCAGATCTGGGATACCGCTGGTCAAGAAAGGTACCGCGCCATTACAAGTGCTTACTATCGTGGGGCAGTTGGTGCACTTCTTGTATATGATGTTACACGCCATGCAACATTTGAGAACGTCGACAGATGGCTGAAAGAACTGCGAAACCACACAGATTCCAACATTGTTGTGATGCTTGTTGGCAACAAAGCAGATCTCCGTCACCTTGTAGCCGTATCAACTGAAGATGGGAAGTCTTATGCCGAGAAAGAATCACTTTATTTCATGGAAACCTCAGCTCTGGAGGCTACTAACGTAGAGAATTCATTTGCTGAAGTTCTGACTCAAATCTACCGTATTGTTAGCAAGAAAGCTGTAGAGGGTGCTGAAAATGGAAACGCGTCTGTGCCGGCCAAAGGAGAGAAAATAGATCTTAAGAATGATGTTTCAGCTTTGAAGAGAGTTGGTTGCTGCTCAAGCTAA
Protein sequence

>Msa0214550

GAFFIFQLNTTLTFSFSLHISLLFLLIATEQIHQRRRRRRRMAGYRADDDYDYLFKVVLIGDSGVGKSNLLSRFTKNEFNLESKSTIGVEFATRTLNVDSKVIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDVTRHATFENVDRWLKELRNHTDSNIVVMLVGNKADLRHLVAVSTEDGKSYAEKESLYFMETSALEATNVENSFAEVLTQIYRIVSKKAVEGAENGNASVPAKGEKIDLKNDVSALKRVGCCSS*