Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1303270 | XP_024628732.1 | 93.855 | 179 | 11 | 0 | 1 | 179 | 1 | 179 | 7.28e-120 | 351 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1303270 | A0A2N9I863 | 73.184 | 179 | 48 | 0 | 1 | 179 | 1 | 179 | 2.37e-92 | 282 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0011930 | Msa1303270 | 0.812126 | 4.959079e-51 | -8.615850e-47 |
| Msa0108390 | Msa1303270 | 0.801762 | 7.727688e-49 | -8.615850e-47 |
| Msa0207350 | Msa1303270 | 0.859954 | 2.892659e-63 | -8.615850e-47 |
| Msa1303270 | Msa1303280 | 0.947854 | 2.984581e-106 | -8.615850e-47 |
| Msa1303270 | Msa1329050 | -0.812411 | 4.296643e-51 | -8.615850e-47 |
| Msa1303270 | Msa1390690 | 0.801496 | 8.760922e-49 | -8.615850e-47 |
| Msa1303270 | Msa1390700 | 0.831501 | 1.610499e-55 | -8.615850e-47 |
| Msa1303270 | Msa1392910 | 0.822196 | 2.696526e-53 | -8.615850e-47 |
| Msa1303270 | Msa1418240 | 0.816084 | 6.635605e-52 | -8.615850e-47 |
| Msa1303270 | Msa1465570 | 0.814114 | 1.816989e-51 | -8.615850e-47 |
| Msa0365100 | Msa1303270 | 0.800325 | 1.519839e-48 | -8.615850e-47 |
| Msa0382370 | Msa1303270 | 0.803131 | 4.035481e-49 | -8.615850e-47 |
| Msa0432160 | Msa1303270 | 0.808849 | 2.529654e-50 | -8.615850e-47 |
| Msa0450400 | Msa1303270 | -0.812789 | 3.552746e-51 | -8.615850e-47 |
| Msa0460590 | Msa1303270 | 0.810019 | 1.419013e-50 | -8.615850e-47 |
| Msa0467100 | Msa1303270 | 0.844286 | 8.306326e-59 | -8.615850e-47 |
| Msa0468410 | Msa1303270 | 0.801926 | 7.151290e-49 | -8.615850e-47 |
| Msa0476680 | Msa1303270 | 0.805420 | 1.346374e-49 | -8.615850e-47 |
| Msa1006010 | Msa1303270 | -0.800435 | 1.442985e-48 | -8.615850e-47 |
| Msa0259210 | Msa1303270 | 0.828077 | 1.098753e-54 | -8.615850e-47 |
| Msa0259220 | Msa1303270 | 0.803948 | 2.731539e-49 | -8.615850e-47 |
| Msa0277420 | Msa1303270 | 0.800701 | 1.273880e-48 | -8.615850e-47 |
| Msa0317970 | Msa1303270 | 0.800098 | 1.690069e-48 | -8.615850e-47 |
| Msa0336310 | Msa1303270 | 0.822220 | 2.661026e-53 | -8.615850e-47 |
| Msa0336320 | Msa1303270 | 0.826522 | 2.590083e-54 | -8.615850e-47 |
| Msa1204100 | Msa1303270 | -0.816225 | 6.171838e-52 | -8.615850e-47 |
| Msa0485240 | Msa1303270 | 0.810022 | 1.417420e-50 | -8.615850e-47 |
| Msa0521010 | Msa1303270 | 0.805522 | 1.281720e-49 | -8.615850e-47 |
| Msa0601950 | Msa1303270 | 0.801463 | 8.900289e-49 | -8.615850e-47 |
| Msa0853520 | Msa1303270 | 0.815949 | 7.112594e-52 | -8.615850e-47 |
| Msa0870520 | Msa1303270 | 0.801206 | 1.004349e-48 | -8.615850e-47 |
| Msa0871720 | Msa1303270 | 0.805615 | 1.225707e-49 | -8.615850e-47 |
| Msa0903710 | Msa1303270 | 0.811247 | 7.703869e-51 | -8.615850e-47 |
| Msa0936380 | Msa1303270 | 0.807374 | 5.214647e-50 | -8.615850e-47 |
| Msa0937020 | Msa1303270 | 0.803046 | 4.201868e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAGGCCCTTAACATTTC+TGG | 0.105015 | 8_3:+66338674 | Msa1303270:CDS |
| TCTTAAGTGCTTCATTTATA+TGG | 0.216688 | 8_3:-66338826 | None:intergenic |
| ATCCAATCATATCTTGCTTT+TGG | 0.220117 | 8_3:-66338932 | None:intergenic |
| TTTGTATACTTCAGAATTAT+AGG | 0.281074 | 8_3:-66338990 | None:intergenic |
| TGCCATAAAAGAAGGAAATA+AGG | 0.335057 | 8_3:+66338760 | Msa1303270:CDS |
| AGTTGTTTCCAGAAATGTTA+AGG | 0.345031 | 8_3:-66338682 | None:intergenic |
| GTTGTTTCCAGAAATGTTAA+GGG | 0.399743 | 8_3:-66338681 | None:intergenic |
| TAATTTAATTTCTTGTCTCC+TGG | 0.400386 | 8_3:+66339147 | Msa1303270:CDS |
| TTTCACGGTATTGTTGTTGC+AGG | 0.408251 | 8_3:-66339105 | None:intergenic |
| CTGGAAACAACTATTTGACT+TGG | 0.414379 | 8_3:+66338693 | Msa1303270:CDS |
| TACTTAGCTGCTGAAGGAAA+TGG | 0.418999 | 8_3:+66338734 | Msa1303270:CDS |
| CTGAACAAAACAATGTGAAC+TGG | 0.441612 | 8_3:+66339173 | Msa1303270:CDS |
| TCTCTCAAAACTTCACTTTG+AGG | 0.457299 | 8_3:+66338658 | Msa1303270:CDS |
| TCCCAAAAGCAAGATATGAT+TGG | 0.468237 | 8_3:+66338930 | Msa1303270:CDS |
| TGAAAGATCCACTTGTGCTC+TGG | 0.468533 | 8_3:+66338864 | Msa1303270:CDS |
| TGCAACAACAATACCGTGAA+AGG | 0.496092 | 8_3:+66339107 | Msa1303270:CDS |
| TTGTTGTTGCAGGAGCATAT+TGG | 0.511014 | 8_3:-66339095 | None:intergenic |
| AGTTTATTCCAGAGCACAAG+TGG | 0.512360 | 8_3:-66338872 | None:intergenic |
| GCAACAACAATACCGTGAAA+GGG | 0.516930 | 8_3:+66339108 | Msa1303270:CDS |
| TGGAAACAACTATTTGACTT+GGG | 0.526488 | 8_3:+66338694 | Msa1303270:CDS |
| AATGGAGATGCCATAAAAGA+AGG | 0.547076 | 8_3:+66338752 | Msa1303270:CDS |
| GAGCATATTGGATGCATGAA+AGG | 0.556835 | 8_3:-66339083 | None:intergenic |
| GAAATGTACTTAGCTGCTGA+AGG | 0.580070 | 8_3:+66338728 | Msa1303270:CDS |
| ACTTCTCAATTAGAATTGTG+TGG | 0.587587 | 8_3:+66339016 | Msa1303270:CDS |
| CAACAACAATACCGTGAAAG+GGG | 0.610582 | 8_3:+66339109 | Msa1303270:CDS |
| CTTCATTTATATGGTGACGA+AGG | 0.627090 | 8_3:-66338817 | None:intergenic |
| CAAGTGGATCTTTCACAGTG+AGG | 0.718603 | 8_3:-66338856 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGTATACTTCAGAATTAT+AGG | - | chr8_3:66338993-66339012 | None:intergenic | 20.0% |
| ! | TCTTAAGTGCTTCATTTATA+TGG | - | chr8_3:66338829-66338848 | None:intergenic | 25.0% |
| !! | TAATTTAATTTCTTGTCTCC+TGG | + | chr8_3:66339147-66339166 | Msa1303270:CDS | 25.0% |
| ! | GTTGTTTCCAGAAATGTTAA+GGG | - | chr8_3:66338684-66338703 | None:intergenic | 30.0% |
| ! | AGTTGTTTCCAGAAATGTTA+AGG | - | chr8_3:66338685-66338704 | None:intergenic | 30.0% |
| ! | TGGAAACAACTATTTGACTT+GGG | + | chr8_3:66338694-66338713 | Msa1303270:CDS | 30.0% |
| TGCCATAAAAGAAGGAAATA+AGG | + | chr8_3:66338760-66338779 | Msa1303270:CDS | 30.0% | |
| !!! | TGCCTTATTTCCTTCTTTTA+TGG | - | chr8_3:66338765-66338784 | None:intergenic | 30.0% |
| !! | TCCAATCATATCTTGCTTTT+GGG | - | chr8_3:66338934-66338953 | None:intergenic | 30.0% |
| ! | ATCCAATCATATCTTGCTTT+TGG | - | chr8_3:66338935-66338954 | None:intergenic | 30.0% |
| ACTTCTCAATTAGAATTGTG+TGG | + | chr8_3:66339016-66339035 | Msa1303270:CDS | 30.0% | |
| TGGTGAAAAAATAGCAGATA+CGG | + | chr8_3:66339036-66339055 | Msa1303270:CDS | 30.0% | |
| !!! | ATTTTTGAAATCCCCTTTCA+CGG | - | chr8_3:66339123-66339142 | None:intergenic | 30.0% |
| TCTCTCAAAACTTCACTTTG+AGG | + | chr8_3:66338658-66338677 | Msa1303270:CDS | 35.0% | |
| CTGGAAACAACTATTTGACT+TGG | + | chr8_3:66338693-66338712 | Msa1303270:CDS | 35.0% | |
| AATGGAGATGCCATAAAAGA+AGG | + | chr8_3:66338752-66338771 | Msa1303270:CDS | 35.0% | |
| !!! | TGCTTTTGCTTTTTGTTGCT+CGG | - | chr8_3:66338789-66338808 | None:intergenic | 35.0% |
| ! | CTTCATTTATATGGTGACGA+AGG | - | chr8_3:66338820-66338839 | None:intergenic | 35.0% |
| !! | AATCATATCTTGCTTTTGGG+AGG | - | chr8_3:66338931-66338950 | None:intergenic | 35.0% |
| TCCCAAAAGCAAGATATGAT+TGG | + | chr8_3:66338930-66338949 | Msa1303270:CDS | 35.0% | |
| CTGAACAAAACAATGTGAAC+TGG | + | chr8_3:66339173-66339192 | Msa1303270:CDS | 35.0% | |
| ! | TTTGAGGCCCTTAACATTTC+TGG | + | chr8_3:66338674-66338693 | Msa1303270:CDS | 40.0% |
| GAAATGTACTTAGCTGCTGA+AGG | + | chr8_3:66338728-66338747 | Msa1303270:CDS | 40.0% | |
| TACTTAGCTGCTGAAGGAAA+TGG | + | chr8_3:66338734-66338753 | Msa1303270:CDS | 40.0% | |
| AGTTTATTCCAGAGCACAAG+TGG | - | chr8_3:66338875-66338894 | None:intergenic | 40.0% | |
| !! | TCTTGCTTTTGGGAGGATAA+TGG | - | chr8_3:66338924-66338943 | None:intergenic | 40.0% |
| GAGCATATTGGATGCATGAA+AGG | - | chr8_3:66339086-66339105 | None:intergenic | 40.0% | |
| ! | TTGTTGTTGCAGGAGCATAT+TGG | - | chr8_3:66339098-66339117 | None:intergenic | 40.0% |
| ! | TTTCACGGTATTGTTGTTGC+AGG | - | chr8_3:66339108-66339127 | None:intergenic | 40.0% |
| TGCAACAACAATACCGTGAA+AGG | + | chr8_3:66339107-66339126 | Msa1303270:CDS | 40.0% | |
| GCAACAACAATACCGTGAAA+GGG | + | chr8_3:66339108-66339127 | Msa1303270:CDS | 40.0% | |
| CAACAACAATACCGTGAAAG+GGG | + | chr8_3:66339109-66339128 | Msa1303270:CDS | 40.0% | |
| !! | CATTGTTTTGTTCAGCAACC+AGG | - | chr8_3:66339168-66339187 | None:intergenic | 40.0% |
| CAAGTGGATCTTTCACAGTG+AGG | - | chr8_3:66338859-66338878 | None:intergenic | 45.0% | |
| TGAAAGATCCACTTGTGCTC+TGG | + | chr8_3:66338864-66338883 | Msa1303270:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 66338650 | 66339210 | 66338650 | ID=Msa1303270;Name=Msa1303270 |
| chr8_3 | mRNA | 66338650 | 66339210 | 66338650 | ID=Msa1303270-mRNA-1;Parent=Msa1303270;Name=Msa1303270-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|186 |
| chr8_3 | exon | 66338650 | 66339210 | 66338650 | ID=Msa1303270-mRNA-1:exon:20150;Parent=Msa1303270-mRNA-1 |
| chr8_3 | CDS | 66338650 | 66339210 | 66338650 | ID=Msa1303270-mRNA-1:cds;Parent=Msa1303270-mRNA-1 |
| Gene Sequence |
| Protein sequence |
