AlfalfaGEDB | Msa1303270

M. sativa cultivar zhongmu-4 / Msa1303270

KEGG: -
GO: -
PFAM: PF14223.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1303270	XP_024628732.1	93.855	179	11	0	1	179	1	179	7.28e-120	351

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1303270	A0A2N9I863	73.184	179	48	0	1	179	1	179	2.37e-92	282

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0011930	Msa1303270	0.812126	4.959079e-51	-8.615850e-47
Msa0108390	Msa1303270	0.801762	7.727688e-49	-8.615850e-47
Msa0207350	Msa1303270	0.859954	2.892659e-63	-8.615850e-47
Msa0259210	Msa1303270	0.828077	1.098753e-54	-8.615850e-47
Msa0259220	Msa1303270	0.803948	2.731539e-49	-8.615850e-47
Msa0277420	Msa1303270	0.800701	1.273880e-48	-8.615850e-47
Msa0317970	Msa1303270	0.800098	1.690069e-48	-8.615850e-47
Msa0336310	Msa1303270	0.822220	2.661026e-53	-8.615850e-47
Msa0336320	Msa1303270	0.826522	2.590083e-54	-8.615850e-47
Msa0365100	Msa1303270	0.800325	1.519839e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 27 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATGGAGATGCCATAAAAGA+AGG	0.547076	8_3:+66338752	Msa1303270:CDS
ACTTCTCAATTAGAATTGTG+TGG	0.587587	8_3:+66339016	Msa1303270:CDS
AGTTGTTTCCAGAAATGTTA+AGG	0.345031	8_3:-66338682	None:intergenic
AGTTTATTCCAGAGCACAAG+TGG	0.512360	8_3:-66338872	None:intergenic
ATCCAATCATATCTTGCTTT+TGG	0.220117	8_3:-66338932	None:intergenic
CAACAACAATACCGTGAAAG+GGG	0.610582	8_3:+66339109	Msa1303270:CDS
CAAGTGGATCTTTCACAGTG+AGG	0.718603	8_3:-66338856	None:intergenic
CTGAACAAAACAATGTGAAC+TGG	0.441612	8_3:+66339173	Msa1303270:CDS
CTGGAAACAACTATTTGACT+TGG	0.414379	8_3:+66338693	Msa1303270:CDS
CTTCATTTATATGGTGACGA+AGG	0.627090	8_3:-66338817	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGCCATAAAAGAAGGAAATA+AGG	+	chr8_3:66338760-66338779	Msa1303270:CDS	30.0%
ACTTCTCAATTAGAATTGTG+TGG	+	chr8_3:66339016-66339035	Msa1303270:CDS	30.0%
TGGTGAAAAAATAGCAGATA+CGG	+	chr8_3:66339036-66339055	Msa1303270:CDS	30.0%
TCTCTCAAAACTTCACTTTG+AGG	+	chr8_3:66338658-66338677	Msa1303270:CDS	35.0%
CTGGAAACAACTATTTGACT+TGG	+	chr8_3:66338693-66338712	Msa1303270:CDS	35.0%
AATGGAGATGCCATAAAAGA+AGG	+	chr8_3:66338752-66338771	Msa1303270:CDS	35.0%
TCCCAAAAGCAAGATATGAT+TGG	+	chr8_3:66338930-66338949	Msa1303270:CDS	35.0%
CTGAACAAAACAATGTGAAC+TGG	+	chr8_3:66339173-66339192	Msa1303270:CDS	35.0%
GAAATGTACTTAGCTGCTGA+AGG	+	chr8_3:66338728-66338747	Msa1303270:CDS	40.0%
TACTTAGCTGCTGAAGGAAA+TGG	+	chr8_3:66338734-66338753	Msa1303270:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	66338650	66339210	66338650	ID=Msa1303270;Name=Msa1303270
chr8_3	mRNA	66338650	66339210	66338650	ID=Msa1303270-mRNA-1;Parent=Msa1303270;Name=Msa1303270-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|186
chr8_3	exon	66338650	66339210	66338650	ID=Msa1303270-mRNA-1:exon:20150;Parent=Msa1303270-mRNA-1
chr8_3	CDS	66338650	66339210	66338650	ID=Msa1303270-mRNA-1:cds;Parent=Msa1303270-mRNA-1

Gene Sequence

>Msa1303270

ATGTCAAATCTCTCAAAACTTCACTTTGAGGCCCTTAACATTTCTGGAAACAACTATTTGACTTGGGCTGTAGATACTGAAATGTACTTAGCTGCTGAAGGAAATGGAGATGCCATAAAAGAAGGAAATAAGGCATCCGAGCAACAAAAAGCAAAAGCATTAATATTCCTTCGTCACCATATAAATGAAGCACTTAAGAATGAATACCTCACTGTGAAAGATCCACTTGTGCTCTGGAATAAACTAAAAGAAAGATATGAGCACTTGAAAGCCATTATCCTCCCAAAAGCAAGATATGATTGGATGCACTTGCGCTTACAAGACTTTAAGTCTGTAACTGCCTATAATTCTGAAGTATACAAAATTACTTCTCAATTAGAATTGTGTGGTGAAAAAATAGCAGATACGGATCTGTTAGAGAAAACATTTTCAACCTTTCATGCATCCAATATGCTCCTGCAACAACAATACCGTGAAAGGGGATTTCAAAAATACTCTAATTTAATTTCTTGTCTCCTGGTTGCTGAACAAAACAATGTGAACTGGAAGTTCAAAAGATAA

Protein sequence

>Msa1303270

MSNLSKLHFEALNISGNNYLTWAVDTEMYLAAEGNGDAIKEGNKASEQQKAKALIFLRHHINEALKNEYLTVKDPLVLWNKLKERYEHLKAIILPKARYDWMHLRLQDFKSVTAYNSEVYKITSQLELCGEKIADTDLLEKTFSTFHASNMLLQQQYRERGFQKYSNLISCLLVAEQNNVNWKFKR*