AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene002877

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002877.t1 MTR_2g022200 100.000 205 0 0 1 205 1 205 1.01e-149 414
MS.gene002877.t1 MTR_4g073560 97.468 79 2 0 117 195 118 196 1.31e-47 155
MS.gene002877.t1 MTR_8g077540 41.566 166 89 2 18 182 17 175 1.20e-38 131
MS.gene002877.t1 MTR_8g077540 41.566 166 89 2 18 182 43 201 3.54e-38 131
MS.gene002877.t1 MTR_5g034130 34.831 178 105 2 5 180 5 173 4.78e-37 127
MS.gene002877.t1 MTR_5g034125 34.831 178 105 2 5 180 5 173 4.78e-37 127
MS.gene002877.t1 MTR_7g109960 34.831 178 105 2 5 180 5 173 5.09e-37 127
MS.gene002877.t1 MTR_6g005820 34.831 178 105 2 5 180 5 173 5.32e-37 127
MS.gene002877.t1 MTR_1g086480 34.831 178 105 2 5 180 5 173 5.38e-37 127
MS.gene002877.t1 MTR_5g016540 34.682 173 102 2 10 180 10 173 2.89e-36 125
MS.gene002877.t1 MTR_4g128910 35.135 185 107 2 4 185 4 178 5.74e-35 122
MS.gene002877.t1 MTR_1g076550 34.973 183 106 2 1 180 1 173 3.16e-32 115
MS.gene002877.t1 MTR_5g015370 32.787 183 110 2 7 182 2 178 1.57e-30 110
MS.gene002877.t1 MTR_5g007810 31.250 192 121 2 1 190 1 183 2.80e-29 107
MS.gene002877.t1 MTR_4g026950 32.727 165 105 1 18 182 1 159 3.32e-29 107
MS.gene002877.t1 MTR_4g077640 31.579 190 119 2 1 188 1 181 3.40e-29 107
MS.gene002877.t1 MTR_5g078090 32.240 183 111 2 7 182 2 178 2.74e-28 105
MS.gene002877.t1 MTR_5g007820 30.102 196 122 3 1 191 1 186 4.44e-27 102
MS.gene002877.t1 MTR_7g112090 37.584 149 81 3 7 155 15 151 2.01e-23 92.8
MS.gene002877.t1 MTR_2g034640 37.267 161 83 4 1 155 3 151 4.22e-23 92.0
MS.gene002877.t1 MTR_4g121860 37.267 161 83 4 1 155 3 151 4.87e-22 89.4
MS.gene002877.t1 MTR_8g077540 37.500 104 58 1 18 121 17 113 7.64e-19 79.3
MS.gene002877.t1 MTR_5g007840 22.467 227 118 4 1 191 1 205 5.02e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002877.t1 AT5G52210 79.798 198 40 0 1 198 1 198 1.13e-115 328
MS.gene002877.t1 AT5G52210 79.798 198 40 0 1 198 1 198 1.13e-115 328
MS.gene002877.t1 AT5G14670 34.831 178 105 2 5 180 5 173 5.00e-37 127
MS.gene002877.t1 AT5G14670 34.831 178 105 2 5 180 5 173 5.00e-37 127
MS.gene002877.t1 AT5G14670 34.831 178 105 2 5 180 5 173 6.29e-37 127
MS.gene002877.t1 AT1G70490 34.831 178 105 2 5 180 5 173 7.38e-37 127
MS.gene002877.t1 AT1G70490 34.831 178 105 2 5 180 5 173 7.38e-37 127
MS.gene002877.t1 AT1G70490 34.831 178 105 2 5 180 5 173 7.38e-37 127
MS.gene002877.t1 AT1G70490 34.831 178 105 2 5 180 5 173 7.38e-37 127
MS.gene002877.t1 AT1G70490 34.831 178 105 2 5 180 5 173 7.38e-37 127
MS.gene002877.t1 AT1G70490 34.831 178 105 2 5 180 5 173 7.38e-37 127
MS.gene002877.t1 AT1G23490 34.831 178 105 2 5 180 5 173 7.38e-37 127
MS.gene002877.t1 AT1G70490 34.831 178 105 2 5 180 5 173 7.38e-37 127
MS.gene002877.t1 AT1G10630 34.831 178 105 2 5 180 5 173 7.46e-37 127
MS.gene002877.t1 AT1G10630 34.831 178 105 2 5 180 5 173 7.46e-37 127
MS.gene002877.t1 AT2G47170 34.831 178 105 2 5 180 5 173 1.05e-36 126
MS.gene002877.t1 AT2G47170 34.831 178 105 2 5 180 5 173 1.05e-36 126
MS.gene002877.t1 AT2G47170 34.831 178 105 2 5 180 5 173 1.05e-36 126
MS.gene002877.t1 AT3G62290 34.831 178 105 2 5 180 5 173 1.31e-36 126
MS.gene002877.t1 AT3G62290 34.831 178 105 2 5 180 5 173 1.31e-36 126
MS.gene002877.t1 AT3G62290 34.831 178 105 2 5 180 5 173 1.31e-36 126
MS.gene002877.t1 AT2G18390 39.759 166 92 2 18 182 17 175 3.15e-36 125
MS.gene002877.t1 AT2G15310 33.500 200 118 3 1 194 1 191 5.95e-35 123
MS.gene002877.t1 AT2G24765 34.783 184 109 2 4 185 4 178 1.31e-34 121
MS.gene002877.t1 AT3G22950 34.831 178 103 2 8 182 8 175 4.24e-32 115
MS.gene002877.t1 AT3G22950 34.831 178 103 2 8 182 8 175 4.24e-32 115
MS.gene002877.t1 AT5G67560 33.333 189 113 2 7 188 2 184 3.05e-31 112
MS.gene002877.t1 AT3G49870 33.708 178 112 1 5 182 7 178 3.66e-31 112
MS.gene002877.t1 AT3G49870 34.783 161 99 1 22 182 5 159 2.21e-29 107
MS.gene002877.t1 AT3G03120 30.918 207 125 3 1 205 1 191 2.34e-29 108
MS.gene002877.t1 AT5G37680 33.333 183 109 2 7 182 2 178 5.87e-29 107
MS.gene002877.t1 AT5G17060 31.053 190 120 2 1 188 1 181 1.18e-28 106
MS.gene002877.t1 AT5G17060 31.053 190 120 2 1 188 1 181 1.18e-28 106
MS.gene002877.t1 AT2G24765 37.778 135 71 2 4 135 4 128 4.42e-26 98.2
MS.gene002877.t1 AT1G02440 29.032 186 113 5 12 186 12 189 2.07e-25 97.8
MS.gene002877.t1 AT5G37680 35.878 131 84 0 52 182 1 131 2.45e-24 93.6
MS.gene002877.t1 AT3G49860 30.120 166 110 1 17 182 11 170 5.22e-24 94.0
MS.gene002877.t1 AT1G02430 27.957 186 115 5 12 186 12 189 3.83e-23 92.0
MS.gene002877.t1 AT4G02080 38.255 149 80 3 7 155 15 151 6.76e-23 91.7
MS.gene002877.t1 AT3G62560 39.041 146 77 3 7 152 15 148 8.99e-23 91.3
MS.gene002877.t1 AT1G56330 37.267 161 83 4 1 155 3 151 1.45e-22 90.5
MS.gene002877.t1 AT3G03120 33.094 139 82 2 1 137 1 130 3.60e-22 88.2
MS.gene002877.t1 AT1G09180 37.179 156 90 3 1 155 3 151 1.19e-21 88.2
MS.gene002877.t1 AT1G02430 27.564 156 98 4 12 160 12 159 1.93e-18 79.0

Find 49 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTGGTCTTCGCTCAATC+TGG 0.301799 2.1:+66002246 MS.gene002877:intron
TCTTCAGATGATACTGCTTC+AGG 0.332255 2.1:-66002703 None:intergenic
CAAATAGAAAACTTGTGTTC+TGG 0.340227 2.1:+66001562 MS.gene002877:CDS
AATGTTGCAGGCTGGGGATC+AGG 0.347707 2.1:+66003212 MS.gene002877:intron
CCAACTGTGGGATTGAATAT+TGG 0.365635 2.1:+66001525 MS.gene002877:CDS
AAATAGAAAACTTGTGTTCT+GGG 0.404046 2.1:+66001563 MS.gene002877:CDS
AAAACTTGTGTTCTGGGACC+TGG 0.431630 2.1:+66001569 MS.gene002877:CDS
GACTGAAATGTTGAGATTGC+GGG 0.443386 2.1:+66003279 MS.gene002877:CDS
GTTCTGGGACCTGGGCGGCC+AGG 0.460687 2.1:+66001578 MS.gene002877:CDS
AAACTTGTGTTCTGGGACCT+GGG 0.461013 2.1:+66001570 MS.gene002877:CDS
CGGGCAGGTGCAATGGGTCC+AGG 0.467778 2.1:+66003298 MS.gene002877:CDS
GTATCTAGATTTAAAGAAGC+TGG 0.477589 2.1:+66002735 MS.gene002877:CDS
GAGATTGCGGGCAGGTGCAA+TGG 0.494160 2.1:+66003291 MS.gene002877:CDS
AGATTGCGGGCAGGTGCAAT+GGG 0.499243 2.1:+66003292 MS.gene002877:CDS
GAAGCCGTGTCCGCATATGA+TGG 0.503479 2.1:+66002778 MS.gene002877:CDS
ACAGTTGGAACAATTCGATC+AGG 0.520181 2.1:-66001510 None:intergenic
CAGGACCTCTAGGCAGGACC+TGG 0.520559 2.1:-66003316 None:intergenic
GCTTGGTCTTCGCTCAATCT+GGG 0.527000 2.1:+66002247 MS.gene002877:intron
ATGTTGCAGGCTGGGGATCA+GGG 0.529063 2.1:+66003213 MS.gene002877:intron
CCAATATTCAATCCCACAGT+TGG 0.529873 2.1:-66001525 None:intergenic
TGATGTCAGGACCTCTAGGC+AGG 0.532450 2.1:-66003322 None:intergenic
GATTGACAAGGCTGGCAAAA+CGG 0.536788 2.1:+66001337 MS.gene002877:CDS
TTGTTTGCTAATATCAAAAG+AGG 0.540730 2.1:-66002538 None:intergenic
GAAGATGCAAAGTCTGCACT+TGG 0.546377 2.1:+66002335 MS.gene002877:CDS
TTTATGATACCTGGCCGCCC+AGG 0.548036 2.1:-66001587 None:intergenic
GAATATTGGTCGAATTGAAG+TGG 0.551287 2.1:+66001539 MS.gene002877:CDS
TGTTTGCTAATATCAAAAGA+GGG 0.557064 2.1:-66002537 None:intergenic
GATCGAATTGTTCCAACTGT+GGG 0.562879 2.1:+66001513 MS.gene002877:CDS
AAGCCGTGTCCGCATATGAT+GGG 0.572837 2.1:+66002779 MS.gene002877:CDS
TTACCCATCATATGCGGACA+CGG 0.580292 2.1:-66002782 None:intergenic
GGACTGAAATGTTGAGATTG+CGG 0.582621 2.1:+66003278 MS.gene002877:CDS
TGATCGAATTGTTCCAACTG+TGG 0.589660 2.1:+66001512 MS.gene002877:CDS
AGAAAAGGTGCTTCGACATG+AGG 0.590643 2.1:+66002501 MS.gene002877:intron
TGGGTCCAGGTCCTGCCTAG+AGG 0.592022 2.1:+66003311 MS.gene002877:CDS
GGAAAGTGCAGAATGGCTTG+TGG 0.602944 2.1:+66003234 MS.gene002877:CDS
TCGGTGTACACAAACGTAGA+AGG 0.604189 2.1:+66001480 MS.gene002877:CDS
TATCATCTGAAGAACTAGCT+CGG 0.614679 2.1:+66002713 MS.gene002877:CDS
CTGGGAGAAATATTATGAAG+AGG 0.615056 2.1:+66002265 MS.gene002877:CDS
GGATCAGGGAAAGTGCAGAA+TGG 0.620734 2.1:+66003227 MS.gene002877:CDS
TTTGCTTGAGAAGATAAAGT+CGG 0.631382 2.1:+66001461 MS.gene002877:CDS
AAGTGATGGAGAGAAGCAAG+AGG 0.637928 2.1:+66003257 MS.gene002877:CDS
CTTCGACATGAGGATCTCAA+AGG 0.639558 2.1:+66002511 MS.gene002877:CDS
GAAATGTTGAGATTGCGGGC+AGG 0.639681 2.1:+66003283 MS.gene002877:CDS
GTTGGAACAATTCGATCAGG+AGG 0.646916 2.1:-66001507 None:intergenic
AGAATGGCTTGTGGAAGTGA+TGG 0.647620 2.1:+66003243 MS.gene002877:CDS
TTTGCATCTTCAAATCGTGA+CGG 0.660305 2.1:-66002323 None:intergenic
GTTTGCTAATATCAAAAGAG+GGG 0.667030 2.1:-66002536 None:intergenic
TCTCACTTACCCATCATATG+CGG 0.684179 2.1:-66002788 None:intergenic
CTTGTGTTCTGGGACCTGGG+CGG 0.788115 2.1:+66001573 MS.gene002877:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCTATAATTATGATATT+TGG + chr2.1:66001635-66001654 MS.gene002877:intron 10.0%
!! ACTAAATTTATACTAATTTA+AGG + chr2.1:66002390-66002409 MS.gene002877:intron 10.0%
!! TTTATCTTATAATATGAAAA+GGG - chr2.1:66002200-66002219 None:intergenic 10.0%
!!! TTTTATCTTATAATATGAAA+AGG - chr2.1:66002201-66002220 None:intergenic 10.0%
!! AGTAGTTTCTTAAAAAATAA+GGG - chr2.1:66001379-66001398 None:intergenic 15.0%
!! ATTATTAATTAATGAGTGAT+AGG + chr2.1:66002994-66003013 MS.gene002877:intron 15.0%
!! TAAGAAAAAAATAACCATTA+AGG + chr2.1:66002890-66002909 MS.gene002877:intron 15.0%
!! AAACATAAAAAATAAGACCT+CGG - chr2.1:66001786-66001805 None:intergenic 20.0%
!! ATATAAAGTAACAGTTCTTA+TGG - chr2.1:66001960-66001979 None:intergenic 20.0%
!! CAGTAGTTTCTTAAAAAATA+AGG - chr2.1:66001380-66001399 None:intergenic 20.0%
!! GAAATGTAATACAAAGAATT+AGG - chr2.1:66003167-66003186 None:intergenic 20.0%
!! TATTGAAAATGAAATGGTAA+TGG - chr2.1:66002851-66002870 None:intergenic 20.0%
!!! TTACCATTTCATTTTCAATA+AGG + chr2.1:66002851-66002870 MS.gene002877:intron 20.0%
! AAATAGAAAACTTGTGTTCT+GGG + chr2.1:66001563-66001582 MS.gene002877:CDS 25.0%
! AACATAAAAAATAAGACCTC+GGG - chr2.1:66001785-66001804 None:intergenic 25.0%
! ATCATTTCATTCTACTTCAA+TGG + chr2.1:66001728-66001747 MS.gene002877:intron 25.0%
! GATCCTTATTGAAAATGAAA+TGG - chr2.1:66002857-66002876 None:intergenic 25.0%
! GTTGCCTATAAATAATAAGT+GGG + chr2.1:66003030-66003049 MS.gene002877:intron 25.0%
! TACTTCATTATGTACCTTAA+TGG - chr2.1:66002907-66002926 None:intergenic 25.0%
! TATGTTGAAATTAAACAGCA+CGG + chr2.1:66002450-66002469 MS.gene002877:intron 25.0%
! TGTTGCCTATAAATAATAAG+TGG + chr2.1:66003029-66003048 MS.gene002877:intron 25.0%
! TTGCCTATAAATAATAAGTG+GGG + chr2.1:66003031-66003050 MS.gene002877:intron 25.0%
! TTTATACTAATTTAAGGAGC+TGG + chr2.1:66002396-66002415 MS.gene002877:intron 25.0%
!! GATGAATTGTTAATTTGACT+TGG + chr2.1:66001657-66001676 MS.gene002877:intron 25.0%
!! TGTTTGCTAATATCAAAAGA+GGG - chr2.1:66002540-66002559 None:intergenic 25.0%
!! TTGTTTGCTAATATCAAAAG+AGG - chr2.1:66002541-66002560 None:intergenic 25.0%
!!! TTTCGTTATTTTATGGACTT+TGG + chr2.1:66001270-66001289 MS.gene002877:CDS 25.0%
AAATAATAAGTGGGGTTGAA+AGG + chr2.1:66003039-66003058 MS.gene002877:intron 30.0%
AATCAAATGTTCGTGCATAT+TGG - chr2.1:66002076-66002095 None:intergenic 30.0%
AATGATCTGTTTACCAGAAA+AGG + chr2.1:66002486-66002505 MS.gene002877:intron 30.0%
AGAATAACACAGAACTTCAA+GGG - chr2.1:66003120-66003139 None:intergenic 30.0%
CAAATAGAAAACTTGTGTTC+TGG + chr2.1:66001562-66001581 MS.gene002877:CDS 30.0%
CAACCCCACTTATTATTTAT+AGG - chr2.1:66003037-66003056 None:intergenic 30.0%
CATAAAAAGGGTGAAATAGA+TGG - chr2.1:66002008-66002027 None:intergenic 30.0%
CTAAACATTTGAGTCAATTG+GGG + chr2.1:66003066-66003085 MS.gene002877:intron 30.0%
GACTATCTATTTATGATACC+TGG - chr2.1:66001599-66001618 None:intergenic 30.0%
GCTAAACATTTGAGTCAATT+GGG + chr2.1:66003065-66003084 MS.gene002877:intron 30.0%
GTATCTAGATTTAAAGAAGC+TGG + chr2.1:66002735-66002754 MS.gene002877:CDS 30.0%
TCAAACTTCAGCCATAAAAA+GGG - chr2.1:66002020-66002039 None:intergenic 30.0%
TGCATACGAACTATGTTATT+TGG + chr2.1:66001981-66002000 MS.gene002877:intron 30.0%
TTCAAACTTCAGCCATAAAA+AGG - chr2.1:66002021-66002040 None:intergenic 30.0%
TTCTCTCTGAACAATTCTTT+GGG + chr2.1:66001856-66001875 MS.gene002877:intron 30.0%
TTTCTCTCTGAACAATTCTT+TGG + chr2.1:66001855-66001874 MS.gene002877:intron 30.0%
TTTGCTTGAGAAGATAAAGT+CGG + chr2.1:66001461-66001480 MS.gene002877:CDS 30.0%
! CAATTGACTCAAATGTTTAG+CGG - chr2.1:66003066-66003085 None:intergenic 30.0%
! CATCTATTTCACCCTTTTTA+TGG + chr2.1:66002006-66002025 MS.gene002877:intron 30.0%
! GTTTGCTAATATCAAAAGAG+GGG - chr2.1:66002539-66002558 None:intergenic 30.0%
!! TTCATTGACTCTAGCTTTAT+GGG + chr2.1:66001693-66001712 MS.gene002877:intron 30.0%
!! TTTATCCTTTGAAATGTTGC+AGG + chr2.1:66003200-66003219 MS.gene002877:intron 30.0%
AAAAAAATCCCCTAGACTAC+AGG - chr2.1:66003143-66003162 None:intergenic 35.0%
AAACACACACTAAGCCAATT+AGG - chr2.1:66001898-66001917 None:intergenic 35.0%
AAATAGGATCACAACTAACG+AGG - chr2.1:66002612-66002631 None:intergenic 35.0%
AACTACTGAACTTTGTCTTG+TGG + chr2.1:66001392-66001411 MS.gene002877:intron 35.0%
ACCAAAGATGGCGAAAAAAT+AGG - chr2.1:66002628-66002647 None:intergenic 35.0%
CGCTAAACATTTGAGTCAAT+TGG + chr2.1:66003064-66003083 MS.gene002877:intron 35.0%
CTGAACATTCTTCTTATTGC+AGG + chr2.1:66002675-66002694 MS.gene002877:intron 35.0%
CTGGGAGAAATATTATGAAG+AGG + chr2.1:66002265-66002284 MS.gene002877:CDS 35.0%
GAATATTGGTCGAATTGAAG+TGG + chr2.1:66001539-66001558 MS.gene002877:CDS 35.0%
GAGAATAACACAGAACTTCA+AGG - chr2.1:66003121-66003140 None:intergenic 35.0%
TATCATCTGAAGAACTAGCT+CGG + chr2.1:66002713-66002732 MS.gene002877:CDS 35.0%
TCCCTGTCCTATAAAATGTT+CGG + chr2.1:66002583-66002602 MS.gene002877:intron 35.0%
TGTTATTCTCCTGTAGTCTA+GGG + chr2.1:66003131-66003150 MS.gene002877:intron 35.0%
TTTGCATCTTCAAATCGTGA+CGG - chr2.1:66002326-66002345 None:intergenic 35.0%
! AACCGAACATTTTATAGGAC+AGG - chr2.1:66002588-66002607 None:intergenic 35.0%
! ACCGAACATTTTATAGGACA+GGG - chr2.1:66002587-66002606 None:intergenic 35.0%
! AGCTTCGTGTACTCATTTTA+GGG + chr2.1:66001315-66001334 MS.gene002877:CDS 35.0%
! CTGATATGTATGTCACTTGA+GGG + chr2.1:66002947-66002966 MS.gene002877:intron 35.0%
! GAGGAAACCGAACATTTTAT+AGG - chr2.1:66002593-66002612 None:intergenic 35.0%
! TCTGATATGTATGTCACTTG+AGG + chr2.1:66002946-66002965 MS.gene002877:intron 35.0%
!! AAATGGTAATGGATCATCAC+AGG - chr2.1:66002840-66002859 None:intergenic 35.0%
!! ACTCATTTTAGGGATTGACA+AGG + chr2.1:66001325-66001344 MS.gene002877:CDS 35.0%
!! GTTCATTGACTCTAGCTTTA+TGG + chr2.1:66001692-66001711 MS.gene002877:intron 35.0%
!!! TCCTATTTTTTCGCCATCTT+TGG + chr2.1:66002624-66002643 MS.gene002877:intron 35.0%
ACAGTTGGAACAATTCGATC+AGG - chr2.1:66001513-66001532 None:intergenic 40.0%
CACTAAGCCAATTAGGTTAG+TGG - chr2.1:66001891-66001910 None:intergenic 40.0%
CCAACTGTGGGATTGAATAT+TGG + chr2.1:66001525-66001544 MS.gene002877:CDS 40.0%
CCAATATTCAATCCCACAGT+TGG - chr2.1:66001528-66001547 None:intergenic 40.0%
GACTGAAATGTTGAGATTGC+GGG + chr2.1:66003279-66003298 MS.gene002877:CDS 40.0%
GATCGAATTGTTCCAACTGT+GGG + chr2.1:66001513-66001532 MS.gene002877:CDS 40.0%
GGACTGAAATGTTGAGATTG+CGG + chr2.1:66003278-66003297 MS.gene002877:CDS 40.0%
GTTATTCTCCTGTAGTCTAG+GGG + chr2.1:66003132-66003151 MS.gene002877:intron 40.0%
GTTGATACACACACCAAAGA+TGG - chr2.1:66002640-66002659 None:intergenic 40.0%
TCTACATTACATAGTTCCCG+AGG + chr2.1:66001766-66001785 MS.gene002877:intron 40.0%
TCTCACTTACCCATCATATG+CGG - chr2.1:66002791-66002810 None:intergenic 40.0%
TCTTCAGATGATACTGCTTC+AGG - chr2.1:66002706-66002725 None:intergenic 40.0%
TGATCGAATTGTTCCAACTG+TGG + chr2.1:66001512-66001531 MS.gene002877:CDS 40.0%
! CTCTGAACAATTCTTTGGGA+AGG + chr2.1:66001860-66001879 MS.gene002877:intron 40.0%
! GAGCTTCGTGTACTCATTTT+AGG + chr2.1:66001314-66001333 MS.gene002877:CDS 40.0%
! GTGTTATTCTCCTGTAGTCT+AGG + chr2.1:66003130-66003149 MS.gene002877:intron 40.0%
!! ATTTTAGGGATTGACAAGGC+TGG + chr2.1:66001329-66001348 MS.gene002877:CDS 40.0%
AATTTCCCCGCTTATCAGCT+TGG + chr2.1:66002230-66002249 MS.gene002877:intron 45.0%
AGTGATAGCAAGATGTGCAG+AGG - chr2.1:66002173-66002192 None:intergenic 45.0%
CTTCGACATGAGGATCTCAA+AGG + chr2.1:66002511-66002530 MS.gene002877:CDS 45.0%
GAAGATGCAAAGTCTGCACT+TGG + chr2.1:66002335-66002354 MS.gene002877:CDS 45.0%
GATTGACAAGGCTGGCAAAA+CGG + chr2.1:66001337-66001356 MS.gene002877:CDS 45.0%
GGCTACTCCACTAACCTAAT+TGG + chr2.1:66001881-66001900 MS.gene002877:intron 45.0%
GGTGTGTGTATCAACAGTGA+TGG + chr2.1:66002645-66002664 MS.gene002877:intron 45.0%
GTTGGAACAATTCGATCAGG+AGG - chr2.1:66001510-66001529 None:intergenic 45.0%
TCGGTGTACACAAACGTAGA+AGG + chr2.1:66001480-66001499 MS.gene002877:CDS 45.0%
TTACCCATCATATGCGGACA+CGG - chr2.1:66002785-66002804 None:intergenic 45.0%
TTGAGTCAATTGGGGCCTTT+GGG + chr2.1:66003074-66003093 MS.gene002877:intron 45.0%
TTTGAGTCAATTGGGGCCTT+TGG + chr2.1:66003073-66003092 MS.gene002877:intron 45.0%
! AAGTGATGGAGAGAAGCAAG+AGG + chr2.1:66003257-66003276 MS.gene002877:CDS 45.0%
! AGAATGGCTTGTGGAAGTGA+TGG + chr2.1:66003243-66003262 MS.gene002877:CDS 45.0%
!! AAAACTTGTGTTCTGGGACC+TGG + chr2.1:66001569-66001588 MS.gene002877:CDS 45.0%
!! AAACTTGTGTTCTGGGACCT+GGG + chr2.1:66001570-66001589 MS.gene002877:CDS 45.0%
!! AGAAAAGGTGCTTCGACATG+AGG + chr2.1:66002501-66002520 MS.gene002877:intron 45.0%
!! CCTTTGAAATGTTGCAGGCT+GGG + chr2.1:66003205-66003224 MS.gene002877:intron 45.0%
!! CTTTGAAATGTTGCAGGCTG+GGG + chr2.1:66003206-66003225 MS.gene002877:intron 45.0%
!! TCATGTCGAAGCACCTTTTC+TGG - chr2.1:66002502-66002521 None:intergenic 45.0%
!! TCCTTTGAAATGTTGCAGGC+TGG + chr2.1:66003204-66003223 MS.gene002877:intron 45.0%
!!! GTTTTTTTATTTTAAATTAT+AGG + chr2.1:66001914-66001933 MS.gene002877:intron 5.0%
AAGCCGTGTCCGCATATGAT+GGG + chr2.1:66002779-66002798 MS.gene002877:CDS 50.0%
CCCAGCCTGCAACATTTCAA+AGG - chr2.1:66003208-66003227 None:intergenic 50.0%
CGAAGACCAAGCTGATAAGC+GGG - chr2.1:66002239-66002258 None:intergenic 50.0%
GAAATGTTGAGATTGCGGGC+AGG + chr2.1:66003283-66003302 MS.gene002877:CDS 50.0%
GAAGACCAAGCTGATAAGCG+GGG - chr2.1:66002238-66002257 None:intergenic 50.0%
GCGAAGACCAAGCTGATAAG+CGG - chr2.1:66002240-66002259 None:intergenic 50.0%
TCCTGCTACTACAAGTGTGC+AGG - chr2.1:66002128-66002147 None:intergenic 50.0%
! AGCTTGGTCTTCGCTCAATC+TGG + chr2.1:66002246-66002265 MS.gene002877:intron 50.0%
! GCTTGGTCTTCGCTCAATCT+GGG + chr2.1:66002247-66002266 MS.gene002877:intron 50.0%
! GGAAAGTGCAGAATGGCTTG+TGG + chr2.1:66003234-66003253 MS.gene002877:CDS 50.0%
! GGATCAGGGAAAGTGCAGAA+TGG + chr2.1:66003227-66003246 MS.gene002877:CDS 50.0%
AATGTTGCAGGCTGGGGATC+AGG + chr2.1:66003212-66003231 MS.gene002877:intron 55.0%
AGATTGCGGGCAGGTGCAAT+GGG + chr2.1:66003292-66003311 MS.gene002877:CDS 55.0%
ATGTTGCAGGCTGGGGATCA+GGG + chr2.1:66003213-66003232 MS.gene002877:intron 55.0%
CAATTGGGGCCTTTGGGCAT+TGG + chr2.1:66003080-66003099 MS.gene002877:intron 55.0%
GAAGCCGTGTCCGCATATGA+TGG + chr2.1:66002778-66002797 MS.gene002877:CDS 55.0%
GCCTGCACACTTGTAGTAGC+AGG + chr2.1:66002124-66002143 MS.gene002877:intron 55.0%
TTTATGATACCTGGCCGCCC+AGG - chr2.1:66001590-66001609 None:intergenic 55.0%
GAGATTGCGGGCAGGTGCAA+TGG + chr2.1:66003291-66003310 MS.gene002877:CDS 60.0%
TGCCCCCTACCAATGCCCAA+AGG - chr2.1:66003092-66003111 None:intergenic 60.0%
!! CTTGTGTTCTGGGACCTGGG+CGG + chr2.1:66001573-66001592 MS.gene002877:CDS 60.0%
!! GGCCTTTGGGCATTGGTAGG+GGG + chr2.1:66003087-66003106 MS.gene002877:intron 60.0%
!! GGGCCTTTGGGCATTGGTAG+GGG + chr2.1:66003086-66003105 MS.gene002877:intron 60.0%
!! GGGGCCTTTGGGCATTGGTA+GGG + chr2.1:66003085-66003104 MS.gene002877:intron 60.0%
!! TGGGGCCTTTGGGCATTGGT+AGG + chr2.1:66003084-66003103 MS.gene002877:intron 60.0%
!! TGGGCATTGGTAGGGGGCAC+AGG + chr2.1:66003093-66003112 MS.gene002877:intron 65.0%
CGGGCAGGTGCAATGGGTCC+AGG + chr2.1:66003298-66003317 MS.gene002877:CDS 70.0%
!! GTTCTGGGACCTGGGCGGCC+AGG + chr2.1:66001578-66001597 MS.gene002877:CDS 75.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 66001266 66003330 66001266 ID=MS.gene002877
chr2.1 mRNA 66001266 66003330 66001266 ID=MS.gene002877.t1;Parent=MS.gene002877
chr2.1 exon 66001266 66001358 66001266 ID=MS.gene002877.t1.exon1;Parent=MS.gene002877.t1
chr2.1 CDS 66001266 66001358 66001266 ID=cds.MS.gene002877.t1;Parent=MS.gene002877.t1
chr2.1 exon 66001459 66001599 66001459 ID=MS.gene002877.t1.exon2;Parent=MS.gene002877.t1
chr2.1 CDS 66001459 66001599 66001459 ID=cds.MS.gene002877.t1;Parent=MS.gene002877.t1
chr2.1 exon 66002248 66002356 66002248 ID=MS.gene002877.t1.exon3;Parent=MS.gene002877.t1
chr2.1 CDS 66002248 66002356 66002248 ID=cds.MS.gene002877.t1;Parent=MS.gene002877.t1
chr2.1 exon 66002503 66002564 66002503 ID=MS.gene002877.t1.exon4;Parent=MS.gene002877.t1
chr2.1 CDS 66002503 66002564 66002503 ID=cds.MS.gene002877.t1;Parent=MS.gene002877.t1
chr2.1 exon 66002697 66002800 66002697 ID=MS.gene002877.t1.exon5;Parent=MS.gene002877.t1
chr2.1 CDS 66002697 66002800 66002697 ID=cds.MS.gene002877.t1;Parent=MS.gene002877.t1
chr2.1 exon 66003222 66003330 66003222 ID=MS.gene002877.t1.exon6;Parent=MS.gene002877.t1
chr2.1 CDS 66003222 66003330 66003222 ID=cds.MS.gene002877.t1;Parent=MS.gene002877.t1
Gene Sequence

>MS.gene002877

ATGTTTTCGTTATTTTATGGACTTTGGCAGTTCATGTTCAGCAAGTTAGAGCTTCGTGTACTCATTTTAGGGATTGACAAGGCTGGCAAAACGGTAATATATCTTTCAATCCCTTATTTTTTAAGAAACTACTGAACTTTGTCTTGTGGCTCTGAGTTGTTTTATAATTGCTATATAATGAATAATTGTGTAGACTTTGCTTGAGAAGATAAAGTCGGTGTACACAAACGTAGAAGGTCTTCCTCCTGATCGAATTGTTCCAACTGTGGGATTGAATATTGGTCGAATTGAAGTGGCAAATAGAAAACTTGTGTTCTGGGACCTGGGCGGCCAGGTATCATAAATAGATAGTCAATACTTATCTTTGTAAATTCTATAATTATGATATTTGGATGAATTGTTAATTTGACTTGGCAAAGTAATTTTGTTCATTGACTCTAGCTTTATGGGCTGCTTGTCAAAATCATTTCATTCTACTTCAATGGCTAATATAATAAACATCTACATTACATAGTTCCCGAGGTCTTATTTTTTATGTTTCTTTATAAAGTTAAAATGCAATTAATTAATGAAAGAACAAGAAACTGTTTTTCTCTCTGAACAATTCTTTGGGAAGGCTACTCCACTAACCTAATTGGCTTAGTGTGTGTTTTTTTATTTTAAATTATAGGTTTGTTATTTTATCTTCAATCCATAAGAACTGTTACTTTATATATGCATACGAACTATGTTATTTGGACCATCTATTTCACCCTTTTTATGGCTGAAGTTTGAATTTACAAGAGATTTTGCGTTTACAAAATTAGTCCAATATGCACGAACATTTGATTCAGTACTTCATTTTTAACTGAAATATAAGCCTGCACACTTGTAGTAGCAGGAATAGAATGTATCATTTTGTGTACCTCTGCACATCTTGCTATCACTATGTCCCTTTTCATATTATAAGATAAAATGTTAATTTAATTTCCCCGCTTATCAGCTTGGTCTTCGCTCAATCTGGGAGAAATATTATGAAGAGGCACATGCTGTTGTATTTGTTATAGACGCATCTTGTCCGTCACGATTTGAAGATGCAAAGTCTGCACTTGGTTAGTGAGACTTGATTTTAGTATTTGTAAATAACTAAATTTATACTAATTTAAGGAGCTGGCTTGCAGTTGCAAGTTTGCAATAGTACTATATATGTTGAAATTAAACAGCACGGTGATAATTTAATAAATGATCTGTTTACCAGAAAAGGTGCTTCGACATGAGGATCTCAAAGGAGCCCCTCTTTTGATATTAGCAAACAAGCAAGTGAGTGTGACTATTCACTCCCTGTCCTATAAAATGTTCGGTTTCCTCGTTAGTTGTGATCCTATTTTTTCGCCATCTTTGGTGTGTGTATCAACAGTGATGGCTGCATGCTGAACATTCTTCTTATTGCAGGATCTTCCTGAAGCAGTATCATCTGAAGAACTAGCTCGGTATCTAGATTTAAAGAAGCTGGACGAAAGAGTCTACATGTTTGAAGCCGTGTCCGCATATGATGGGTAAGTGAGAAGTTGTATAAAATTTTCAAATCTTAACCTGTGATGATCCATTACCATTTCATTTTCAATAAGGATCGAGATATCTGATGTAAGAAAAAAATAACCATTAAGGTACATAATGAAGTACTTACTGAATGAACTTTATTCTGATATGTATGTCACTTGAGGGAATTAGTTAGTTAGTCTGTTTGTTATTATTAATTAATGAGTGATAGGAAAAATAAGACATGTTGCCTATAAATAATAAGTGGGGTTGAAAGGACCGCTAAACATTTGAGTCAATTGGGGCCTTTGGGCATTGGTAGGGGGCACAGGACCCTTGAAGTTCTGTGTTATTCTCCTGTAGTCTAGGGGATTTTTTTTCCTAATTCTTTGTATTACATTTCTAGTTCACATCACTTTATCCTTTGAAATGTTGCAGGCTGGGGATCAGGGAAAGTGCAGAATGGCTTGTGGAAGTGATGGAGAGAAGCAAGAGGACTGAAATGTTGAGATTGCGGGCAGGTGCAATGGGTCCAGGTCCTGCCTAG
Protein sequence

>MS.gene002877.t1

MFSLFYGLWQFMFSKLELRVLILGIDKAGKTTLLEKIKSVYTNVEGLPPDRIVPTVGLNIGRIEVANRKLVFWDLGGQLGLRSIWEKYYEEAHAVVFVIDASCPSRFEDAKSALEKVLRHEDLKGAPLLILANKQDLPEAVSSEELARYLDLKKLDERVYMFEAVSAYDGLGIRESAEWLVEVMERSKRTEMLRLRAGAMGPGPA