Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53478.t1 | RZB99279.1 | 100 | 181 | 0 | 0 | 1 | 181 | 17 | 197 | 3.60E-98 | 367.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53478.t1 | P51823 | 99.4 | 181 | 1 | 0 | 1 | 181 | 1 | 181 | 2.6e-100 | 365.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53478.t1 | A0A1U7V2M3 | 100.0 | 181 | 0 | 0 | 1 | 181 | 1 | 181 | 2.6e-98 | 367.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050320 | MS.gene53478 | 0.801247 | 9.85E-49 | -1.69E-46 |
| MS.gene050752 | MS.gene53478 | 0.836323 | 1.00E-56 | -1.69E-46 |
| MS.gene050838 | MS.gene53478 | 0.824532 | 7.67E-54 | -1.69E-46 |
| MS.gene050955 | MS.gene53478 | 0.80741 | 5.12E-50 | -1.69E-46 |
| MS.gene051003 | MS.gene53478 | 0.847448 | 1.15E-59 | -1.69E-46 |
| MS.gene051234 | MS.gene53478 | 0.823393 | 1.42E-53 | -1.69E-46 |
| MS.gene051748 | MS.gene53478 | 0.803783 | 2.96E-49 | -1.69E-46 |
| MS.gene053109 | MS.gene53478 | 0.812177 | 4.83E-51 | -1.69E-46 |
| MS.gene054403 | MS.gene53478 | 0.831831 | 1.34E-55 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53478.t1 | MTR_5g034130 | 100.000 | 181 | 0 | 0 | 1 | 181 | 1 | 181 | 2.62e-135 | 375 |
| MS.gene53478.t1 | MTR_5g034125 | 100.000 | 181 | 0 | 0 | 1 | 181 | 1 | 181 | 2.62e-135 | 375 |
| MS.gene53478.t1 | MTR_1g086480 | 99.448 | 181 | 1 | 0 | 1 | 181 | 1 | 181 | 6.88e-135 | 374 |
| MS.gene53478.t1 | MTR_6g005820 | 99.448 | 181 | 1 | 0 | 1 | 181 | 1 | 181 | 9.56e-135 | 374 |
| MS.gene53478.t1 | MTR_7g109960 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 6.38e-134 | 372 |
| MS.gene53478.t1 | MTR_5g016540 | 87.222 | 180 | 23 | 0 | 1 | 180 | 1 | 180 | 1.47e-120 | 338 |
| MS.gene53478.t1 | MTR_4g128910 | 63.277 | 177 | 65 | 0 | 1 | 177 | 1 | 177 | 3.37e-84 | 246 |
| MS.gene53478.t1 | MTR_5g007810 | 61.582 | 177 | 68 | 0 | 1 | 177 | 1 | 177 | 4.35e-83 | 244 |
| MS.gene53478.t1 | MTR_4g077640 | 60.452 | 177 | 70 | 0 | 1 | 177 | 1 | 177 | 2.31e-80 | 237 |
| MS.gene53478.t1 | MTR_5g007820 | 55.495 | 182 | 79 | 1 | 1 | 180 | 1 | 182 | 2.90e-75 | 224 |
| MS.gene53478.t1 | MTR_1g076550 | 51.934 | 181 | 87 | 0 | 1 | 181 | 1 | 181 | 1.18e-67 | 204 |
| MS.gene53478.t1 | MTR_5g007840 | 42.723 | 213 | 77 | 2 | 1 | 180 | 1 | 201 | 8.01e-57 | 177 |
| MS.gene53478.t1 | MTR_8g077540 | 48.485 | 165 | 82 | 3 | 18 | 180 | 17 | 180 | 1.01e-49 | 159 |
| MS.gene53478.t1 | MTR_8g077540 | 48.485 | 165 | 82 | 3 | 18 | 180 | 43 | 206 | 1.51e-49 | 159 |
| MS.gene53478.t1 | MTR_2g022200 | 34.831 | 178 | 105 | 2 | 5 | 173 | 5 | 180 | 4.22e-37 | 127 |
| MS.gene53478.t1 | MTR_5g078090 | 34.091 | 176 | 113 | 2 | 1 | 173 | 1 | 176 | 1.05e-30 | 110 |
| MS.gene53478.t1 | MTR_5g015370 | 32.065 | 184 | 122 | 2 | 1 | 181 | 1 | 184 | 5.42e-30 | 108 |
| MS.gene53478.t1 | MTR_8g077540 | 51.020 | 98 | 46 | 2 | 18 | 114 | 17 | 113 | 1.85e-28 | 103 |
| MS.gene53478.t1 | MTR_4g026950 | 31.847 | 157 | 106 | 1 | 18 | 173 | 1 | 157 | 5.08e-26 | 97.8 |
| MS.gene53478.t1 | MTR_2g034640 | 31.638 | 177 | 105 | 2 | 15 | 177 | 18 | 192 | 1.01e-24 | 95.5 |
| MS.gene53478.t1 | MTR_7g112090 | 31.073 | 177 | 106 | 2 | 15 | 177 | 18 | 192 | 2.77e-24 | 94.4 |
| MS.gene53478.t1 | MTR_4g121860 | 31.638 | 177 | 105 | 2 | 15 | 177 | 18 | 192 | 7.47e-23 | 90.5 |
| MS.gene53478.t1 | MTR_1g062760 | 34.118 | 85 | 52 | 1 | 18 | 98 | 15 | 99 | 2.54e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53478.t1 | AT5G14670 | 98.895 | 181 | 2 | 0 | 1 | 181 | 1 | 181 | 1.28e-133 | 371 |
| MS.gene53478.t1 | AT5G14670 | 98.895 | 181 | 2 | 0 | 1 | 181 | 1 | 181 | 1.28e-133 | 371 |
| MS.gene53478.t1 | AT1G10630 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 1.56e-133 | 371 |
| MS.gene53478.t1 | AT1G10630 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 1.56e-133 | 371 |
| MS.gene53478.t1 | AT3G62290 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 2.34e-133 | 370 |
| MS.gene53478.t1 | AT3G62290 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 2.34e-133 | 370 |
| MS.gene53478.t1 | AT3G62290 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 2.34e-133 | 370 |
| MS.gene53478.t1 | AT1G70490 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 2.78e-133 | 370 |
| MS.gene53478.t1 | AT1G70490 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 2.78e-133 | 370 |
| MS.gene53478.t1 | AT1G70490 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 2.78e-133 | 370 |
| MS.gene53478.t1 | AT1G70490 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 2.78e-133 | 370 |
| MS.gene53478.t1 | AT1G70490 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 2.78e-133 | 370 |
| MS.gene53478.t1 | AT1G70490 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 2.78e-133 | 370 |
| MS.gene53478.t1 | AT1G23490 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 2.78e-133 | 370 |
| MS.gene53478.t1 | AT1G70490 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 2.78e-133 | 370 |
| MS.gene53478.t1 | AT2G47170 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 3.91e-133 | 370 |
| MS.gene53478.t1 | AT2G47170 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 3.91e-133 | 370 |
| MS.gene53478.t1 | AT2G47170 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 3.91e-133 | 370 |
| MS.gene53478.t1 | AT5G14670 | 98.343 | 181 | 3 | 0 | 1 | 181 | 1 | 181 | 1.05e-132 | 369 |
| MS.gene53478.t1 | AT2G15310 | 67.403 | 181 | 59 | 0 | 1 | 181 | 1 | 181 | 2.79e-91 | 265 |
| MS.gene53478.t1 | AT2G24765 | 62.147 | 177 | 67 | 0 | 1 | 177 | 1 | 177 | 1.42e-84 | 247 |
| MS.gene53478.t1 | AT5G17060 | 58.659 | 179 | 74 | 0 | 1 | 179 | 1 | 179 | 3.19e-81 | 239 |
| MS.gene53478.t1 | AT5G17060 | 58.659 | 179 | 74 | 0 | 1 | 179 | 1 | 179 | 3.19e-81 | 239 |
| MS.gene53478.t1 | AT3G03120 | 60.452 | 177 | 70 | 0 | 1 | 177 | 1 | 177 | 4.48e-81 | 239 |
| MS.gene53478.t1 | AT3G22950 | 53.039 | 181 | 85 | 0 | 1 | 181 | 1 | 181 | 1.15e-70 | 212 |
| MS.gene53478.t1 | AT3G22950 | 53.039 | 181 | 85 | 0 | 1 | 181 | 1 | 181 | 1.15e-70 | 212 |
| MS.gene53478.t1 | AT2G24765 | 64.844 | 128 | 45 | 0 | 1 | 128 | 1 | 128 | 1.19e-60 | 185 |
| MS.gene53478.t1 | AT3G03120 | 63.077 | 130 | 48 | 0 | 1 | 130 | 1 | 130 | 4.95e-60 | 183 |
| MS.gene53478.t1 | AT1G02430 | 48.936 | 188 | 82 | 5 | 1 | 176 | 1 | 186 | 9.40e-57 | 177 |
| MS.gene53478.t1 | AT1G02440 | 46.237 | 186 | 90 | 4 | 1 | 176 | 1 | 186 | 7.38e-56 | 175 |
| MS.gene53478.t1 | AT2G18390 | 48.485 | 165 | 82 | 3 | 18 | 180 | 17 | 180 | 3.51e-49 | 157 |
| MS.gene53478.t1 | AT1G02430 | 47.205 | 161 | 75 | 4 | 1 | 153 | 1 | 159 | 5.08e-45 | 146 |
| MS.gene53478.t1 | AT5G52210 | 34.091 | 176 | 109 | 1 | 5 | 173 | 5 | 180 | 6.65e-36 | 124 |
| MS.gene53478.t1 | AT5G52210 | 34.091 | 176 | 109 | 1 | 5 | 173 | 5 | 180 | 6.65e-36 | 124 |
| MS.gene53478.t1 | AT3G49870 | 34.091 | 176 | 113 | 2 | 1 | 173 | 1 | 176 | 3.21e-32 | 114 |
| MS.gene53478.t1 | AT5G67560 | 33.742 | 163 | 107 | 1 | 12 | 173 | 14 | 176 | 2.52e-31 | 112 |
| MS.gene53478.t1 | AT5G37680 | 33.742 | 163 | 107 | 1 | 12 | 173 | 14 | 176 | 4.83e-30 | 108 |
| MS.gene53478.t1 | AT3G49870 | 33.121 | 157 | 104 | 1 | 18 | 173 | 1 | 157 | 5.25e-29 | 105 |
| MS.gene53478.t1 | AT3G49860 | 32.530 | 166 | 111 | 1 | 16 | 180 | 10 | 175 | 2.42e-28 | 104 |
| MS.gene53478.t1 | AT1G02440 | 33.766 | 154 | 66 | 3 | 1 | 153 | 1 | 119 | 1.15e-26 | 98.6 |
| MS.gene53478.t1 | AT1G09180 | 32.961 | 179 | 104 | 2 | 13 | 177 | 16 | 192 | 2.74e-25 | 97.1 |
| MS.gene53478.t1 | AT3G62560 | 31.638 | 177 | 105 | 2 | 15 | 177 | 18 | 192 | 1.22e-24 | 95.1 |
| MS.gene53478.t1 | AT5G37680 | 32.812 | 128 | 86 | 0 | 46 | 173 | 2 | 129 | 4.55e-24 | 92.0 |
| MS.gene53478.t1 | AT1G56330 | 31.638 | 177 | 105 | 2 | 15 | 177 | 18 | 192 | 4.74e-24 | 93.6 |
| MS.gene53478.t1 | AT4G02080 | 31.073 | 177 | 106 | 2 | 15 | 177 | 18 | 192 | 5.17e-24 | 93.6 |
Find 54 sgRNAs with CRISPR-Local
Find 177 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCAACAACATTGCCAATA+AGG | 0.283068 | 5.3:+23182440 | MS.gene53478:CDS |
| GAAACCAATAGCTACCTTAT+TGG | 0.327478 | 5.3:-23182454 | None:intergenic |
| ATATAAGACCCTGAGTGTTC+TGG | 0.338828 | 5.3:-23181502 | None:intergenic |
| AGAACATTAGCTTCACTGTT+TGG | 0.346877 | 5.3:+23181310 | MS.gene53478:CDS |
| GAACATTAGCTTCACTGTTT+GGG | 0.346980 | 5.3:+23181311 | MS.gene53478:CDS |
| CAGTCGGTTATTTGCGAAGA+AGG | 0.396684 | 5.3:+23180438 | MS.gene53478:CDS |
| CTCAGCTGCATTCATTGCAT+TGG | 0.411804 | 5.3:-23182228 | None:intergenic |
| CAGAGCACTTGTGCCACCTC+TGG | 0.413296 | 5.3:+23182387 | MS.gene53478:CDS |
| TACCTTATTGGCAATGTTGT+TGG | 0.415485 | 5.3:-23182442 | None:intergenic |
| AGCTTCACTGTTTGGGATGT+CGG | 0.421764 | 5.3:+23181318 | MS.gene53478:CDS |
| CCAAGAAACTTACCGATAGT+AGG | 0.449062 | 5.3:-23180550 | None:intergenic |
| TCAGCTGCATTCATTGCATT+GGG | 0.455488 | 5.3:-23182227 | None:intergenic |
| TTGAATAAAATACCTTGTCC+TGG | 0.462109 | 5.3:-23181344 | None:intergenic |
| GGAGGGTCTTTATGAAGGTC+TGG | 0.465424 | 5.3:+23182410 | MS.gene53478:CDS |
| AGCTGAGATTACTGACAAGC+TGG | 0.467998 | 5.3:+23182244 | MS.gene53478:CDS |
| CACTTGTGCCACCTCTGGGG+AGG | 0.474754 | 5.3:+23182392 | MS.gene53478:CDS |
| ACTTGTGCCACCTCTGGGGA+GGG | 0.483489 | 5.3:+23182393 | MS.gene53478:CDS |
| CATCCTATGTAGCTCATCTC+TGG | 0.489228 | 5.3:-23181564 | None:intergenic |
| TGTTTGGGATGTCGGAGGCC+AGG | 0.494393 | 5.3:+23181326 | MS.gene53478:CDS |
| TTGTCGTTGACAGCAATGAC+AGG | 0.500098 | 5.3:+23181525 | MS.gene53478:CDS |
| ATTCTCTACAAGCTCAAGCT+TGG | 0.503263 | 5.3:+23180508 | MS.gene53478:CDS |
| GTCTTTATGAAGGTCTGGAC+TGG | 0.504629 | 5.3:+23182415 | MS.gene53478:CDS |
| ATTGGGAAGATCTTGTTTGT+TGG | 0.504874 | 5.3:-23182210 | None:intergenic |
| TTCTCCAGATCCGTCCCTTG+TGG | 0.505460 | 5.3:+23181468 | MS.gene53478:intron |
| AGAGCACTTGTGCCACCTCT+GGG | 0.506189 | 5.3:+23182388 | MS.gene53478:CDS |
| AAGCTTGAGCTTGTAGAGAA+TGG | 0.532263 | 5.3:-23180506 | None:intergenic |
| TCTGGGGAGGGTCTTTATGA+AGG | 0.537610 | 5.3:+23182405 | MS.gene53478:CDS |
| ACTCTCTCAGACAGCGCCAC+TGG | 0.541563 | 5.3:+23182273 | MS.gene53478:CDS |
| CAAGAAACTTACCGATAGTA+GGG | 0.545597 | 5.3:-23180549 | None:intergenic |
| AGGCCAGAGATGAGCTACAT+AGG | 0.552666 | 5.3:+23181561 | MS.gene53478:CDS |
| GAAGGAAATGCGAATTCTGA+TGG | 0.554686 | 5.3:+23180456 | MS.gene53478:CDS |
| ATGGTTGGTCTCGATGCAGC+TGG | 0.564897 | 5.3:+23180475 | MS.gene53478:CDS |
| CATTCACGAAGCTGTTCAGT+CGG | 0.566942 | 5.3:+23180422 | MS.gene53478:CDS |
| TCCAGATCCGTCCCTTGTGG+AGG | 0.578144 | 5.3:+23181471 | MS.gene53478:intron |
| CTGGAAGTAGTGCCTCCACA+AGG | 0.582344 | 5.3:-23181483 | None:intergenic |
| GAAATGCGAATTCTGATGGT+TGG | 0.586975 | 5.3:+23180460 | MS.gene53478:CDS |
| AGTAGTGCCTCCACAAGGGA+CGG | 0.589421 | 5.3:-23181478 | None:intergenic |
| GTCACCACCATCCCTACTAT+CGG | 0.594267 | 5.3:+23180538 | MS.gene53478:CDS |
| GCACTACTTCCAGAACACTC+AGG | 0.597040 | 5.3:+23181493 | MS.gene53478:CDS |
| TGACAGGGATAGAGTTGTTG+AGG | 0.602509 | 5.3:+23181541 | MS.gene53478:CDS |
| CACTACTTCCAGAACACTCA+GGG | 0.606230 | 5.3:+23181494 | MS.gene53478:CDS |
| GGATGTCGGAGGCCAGGACA+AGG | 0.616890 | 5.3:+23181332 | MS.gene53478:CDS |
| AAACTTACCGATAGTAGGGA+TGG | 0.618750 | 5.3:-23180545 | None:intergenic |
| TTCACTGTTTGGGATGTCGG+AGG | 0.622949 | 5.3:+23181321 | MS.gene53478:CDS |
| TGTCGTTGACAGCAATGACA+GGG | 0.631238 | 5.3:+23181526 | MS.gene53478:CDS |
| AGGGTTCAATGTGGAGACTG+TGG | 0.633133 | 5.3:+23181281 | MS.gene53478:intron |
| GCTACATAGGATGTTGAATG+AGG | 0.633182 | 5.3:+23181574 | MS.gene53478:CDS |
| ATAAAGACCCTCCCCAGAGG+TGG | 0.635325 | 5.3:-23182400 | None:intergenic |
| CTTGAGCTTGTAGAGAATGG+TGG | 0.640954 | 5.3:-23180503 | None:intergenic |
| GCTGAGATTACTGACAAGCT+GGG | 0.649547 | 5.3:+23182245 | MS.gene53478:CDS |
| CTTACCGATAGTAGGGATGG+TGG | 0.656172 | 5.3:-23180542 | None:intergenic |
| GAGCACTTGTGCCACCTCTG+GGG | 0.692264 | 5.3:+23182389 | MS.gene53478:CDS |
| TTCATAAAGACCCTCCCCAG+AGG | 0.695022 | 5.3:-23182403 | None:intergenic |
| TGGAAGTAGTGCCTCCACAA+GGG | 0.700224 | 5.3:-23181482 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTTTATATTTTAATTTA+GGG | + | chr5.3:23181262-23181281 | MS.gene53478:intron | 0.0% |
| !!! | TTTATTTTATATTTTAATTT+AGG | + | chr5.3:23181261-23181280 | MS.gene53478:intron | 0.0% |
| !! | ACTTCAAAAAAAAAAAAAAA+AGG | - | chr5.3:23182717-23182736 | None:intergenic | 10.0% |
| !! | CTTCAAAAAAAAAAAAAAAA+GGG | - | chr5.3:23182716-23182735 | None:intergenic | 10.0% |
| !! | TAAAAAATAAAAATAGAGAA+GGG | - | chr5.3:23182482-23182501 | None:intergenic | 10.0% |
| !! | TTAAAAAATAAAAATAGAGA+AGG | - | chr5.3:23182483-23182502 | None:intergenic | 10.0% |
| !!! | AATTGGAAATATATTAATTT+TGG | - | chr5.3:23182656-23182675 | None:intergenic | 10.0% |
| !!! | TTTTTTTTCAAATAATTATC+GGG | - | chr5.3:23180671-23180690 | None:intergenic | 10.0% |
| !! | AAATTGTATAAATGTGTATA+TGG | + | chr5.3:23182628-23182647 | MS.gene53478:intron | 15.0% |
| !! | AATTGTATAAATGTGTATAT+GGG | + | chr5.3:23182629-23182648 | MS.gene53478:intron | 15.0% |
| !! | GATTTCATTGTTAAATATAA+AGG | - | chr5.3:23180900-23180919 | None:intergenic | 15.0% |
| !!! | AGACTAATTTATTTACTTTT+TGG | + | chr5.3:23182590-23182609 | MS.gene53478:intron | 15.0% |
| !!! | ATGTTTAATTTAAACTTGAT+GGG | + | chr5.3:23181126-23181145 | MS.gene53478:intron | 15.0% |
| !! | AATCAAAATTCATTTCAAAC+AGG | - | chr5.3:23180623-23180642 | None:intergenic | 20.0% |
| !! | ACAATATATGACATAAAGTT+TGG | - | chr5.3:23180923-23180942 | None:intergenic | 20.0% |
| !! | ATTATCAAATTATTTACCAG+TGG | - | chr5.3:23182292-23182311 | None:intergenic | 20.0% |
| !! | ATTTATTGAGCATTTCAAAT+AGG | - | chr5.3:23182751-23182770 | None:intergenic | 20.0% |
| !! | CAATATATGACATAAAGTTT+GGG | - | chr5.3:23180922-23180941 | None:intergenic | 20.0% |
| !! | CATCAAGTTTAAATTAAACA+TGG | - | chr5.3:23181127-23181146 | None:intergenic | 20.0% |
| !! | TAAACAATCATTGAGTTATA+TGG | - | chr5.3:23180824-23180843 | None:intergenic | 20.0% |
| !! | TACAAGAACTAGAAATATTA+TGG | + | chr5.3:23182677-23182696 | MS.gene53478:intron | 20.0% |
| !! | TACTTATGCAATACTTATTA+GGG | + | chr5.3:23182874-23182893 | MS.gene53478:intron | 20.0% |
| !!! | ATTTCTAGTTCTTGTAAAAT+TGG | - | chr5.3:23182673-23182692 | None:intergenic | 20.0% |
| !!! | CATGTTTAATTTAAACTTGA+TGG | + | chr5.3:23181125-23181144 | MS.gene53478:intron | 20.0% |
| !!! | TAATAAGTATTGCATAAGTA+GGG | - | chr5.3:23182874-23182893 | None:intergenic | 20.0% |
| !!! | TAATTAATCCATGTTTTTAC+TGG | + | chr5.3:23181916-23181935 | MS.gene53478:intron | 20.0% |
| !!! | TACTTTTCTTATATTCAGTT+TGG | + | chr5.3:23181597-23181616 | MS.gene53478:intron | 20.0% |
| ! | AAAATAAAAATAGAGAAGGG+AGG | - | chr5.3:23182479-23182498 | None:intergenic | 25.0% |
| ! | AATATGATCCAGTAAAAACA+TGG | - | chr5.3:23181927-23181946 | None:intergenic | 25.0% |
| ! | AATTTGTAGTTTGATAGACA+TGG | + | chr5.3:23182786-23182805 | MS.gene53478:intron | 25.0% |
| ! | ACTTATGCAATACTTATTAG+GGG | + | chr5.3:23182875-23182894 | MS.gene53478:intron | 25.0% |
| ! | ATAATGCTACATGTAGTATT+TGG | + | chr5.3:23182307-23182326 | MS.gene53478:intron | 25.0% |
| ! | ATGGATGAAATTACAAGTTA+AGG | - | chr5.3:23181108-23181127 | None:intergenic | 25.0% |
| ! | CTACTTATGCAATACTTATT+AGG | + | chr5.3:23182873-23182892 | MS.gene53478:intron | 25.0% |
| ! | GTCTACTTCAATGTATAAAA+TGG | + | chr5.3:23182053-23182072 | MS.gene53478:intron | 25.0% |
| ! | GTTAAGTTCATTCATGTTTA+TGG | + | chr5.3:23181073-23181092 | MS.gene53478:intron | 25.0% |
| ! | TCAACTATAATGACTTTGTT+TGG | + | chr5.3:23180984-23181003 | MS.gene53478:intron | 25.0% |
| ! | TGGATGAAATTACAAGTTAA+GGG | - | chr5.3:23181107-23181126 | None:intergenic | 25.0% |
| !! | CTAATAAGTATTGCATAAGT+AGG | - | chr5.3:23182875-23182894 | None:intergenic | 25.0% |
| !! | GATAAAATCAACATTTTCCT+GGG | - | chr5.3:23182523-23182542 | None:intergenic | 25.0% |
| !! | TTACTTAGTAGTCTTTTTGT+GGG | + | chr5.3:23182903-23182922 | MS.gene53478:intron | 25.0% |
| !! | TTTACTTAGTAGTCTTTTTG+TGG | + | chr5.3:23182902-23182921 | MS.gene53478:intron | 25.0% |
| !!! | TTTTAATTTAGGGTTCAATG+TGG | + | chr5.3:23181272-23181291 | MS.gene53478:intron | 25.0% |
| ATCAGAAGCAGTAAATTCAT+AGG | - | chr5.3:23182018-23182037 | None:intergenic | 30.0% | |
| ATTAACAGCAACAATGATGA+AGG | - | chr5.3:23181900-23181919 | None:intergenic | 30.0% | |
| GAGTTGAGATAGAAAGATAT+AGG | + | chr5.3:23181726-23181745 | MS.gene53478:intron | 30.0% | |
| TAAAACACGAGAAAGAATTG+AGG | + | chr5.3:23183052-23183071 | MS.gene53478:intron | 30.0% | |
| TAACATTGAATGTGATCAAG+TGG | + | chr5.3:23180842-23180861 | MS.gene53478:intron | 30.0% | |
| TATGAGTGAAAACGTCTAAA+TGG | - | chr5.3:23181662-23181681 | None:intergenic | 30.0% | |
| TCAGAAGCAGTAAATTCATA+GGG | - | chr5.3:23182017-23182036 | None:intergenic | 30.0% | |
| TTAACAGCAACAATGATGAA+GGG | - | chr5.3:23181899-23181918 | None:intergenic | 30.0% | |
| TTCTAGATCATAATGTTCAC+TGG | + | chr5.3:23181817-23181836 | MS.gene53478:intron | 30.0% | |
| TTGAATAAAATACCTTGTCC+TGG | - | chr5.3:23181347-23181366 | None:intergenic | 30.0% | |
| TTTATAGCCAAGAACTAAGA+AGG | - | chr5.3:23183036-23183055 | None:intergenic | 30.0% | |
| ! | CTGTGTATTAATCTGTCTTT+TGG | + | chr5.3:23181959-23181978 | MS.gene53478:intron | 30.0% |
| ! | GGATAAAATCAACATTTTCC+TGG | - | chr5.3:23182524-23182543 | None:intergenic | 30.0% |
| ! | TACTTAGTAGTCTTTTTGTG+GGG | + | chr5.3:23182904-23182923 | MS.gene53478:intron | 30.0% |
| ! | TCTCATTTGAAATATGCGTT+TGG | + | chr5.3:23181031-23181050 | MS.gene53478:intron | 30.0% |
| ! | TCTTTTGGTACTTGAATGAT+CGG | + | chr5.3:23181974-23181993 | MS.gene53478:intron | 30.0% |
| ! | TGCAATAATTGAGTGCTTTT+AGG | + | chr5.3:23182548-23182567 | MS.gene53478:intron | 30.0% |
| ! | TTGCAAGTTTTGCTTATGTA+TGG | + | chr5.3:23180642-23180661 | MS.gene53478:intron | 30.0% |
| AATATGTTTAGGTGCTCAGA+AGG | - | chr5.3:23182939-23182958 | None:intergenic | 35.0% | |
| AATGAAGACGAAATAGAAGC+AGG | - | chr5.3:23182965-23182984 | None:intergenic | 35.0% | |
| AGCACTCAATTATTGCATCT+TGG | - | chr5.3:23182545-23182564 | None:intergenic | 35.0% | |
| ATATGTTTAGGTGCTCAGAA+GGG | - | chr5.3:23182938-23182957 | None:intergenic | 35.0% | |
| ATGCAATACTTATTAGGGGT+TGG | + | chr5.3:23182879-23182898 | MS.gene53478:intron | 35.0% | |
| ATTGCCAATAAGGTAGCTAT+TGG | + | chr5.3:23182450-23182469 | MS.gene53478:intron | 35.0% | |
| CAAGAAACTTACCGATAGTA+GGG | - | chr5.3:23180552-23180571 | None:intergenic | 35.0% | |
| CACACAAATAATTGCCTACA+AGG | - | chr5.3:23180779-23180798 | None:intergenic | 35.0% | |
| CTCAACTCTCAACAAATTTG+TGG | - | chr5.3:23181714-23181733 | None:intergenic | 35.0% | |
| GAAACCAATAGCTACCTTAT+TGG | - | chr5.3:23182457-23182476 | None:intergenic | 35.0% | |
| GAACAAAAAAGAGAAGAGCA+TGG | - | chr5.3:23182109-23182128 | None:intergenic | 35.0% | |
| GAAGCAGGTAGAATATGTTT+AGG | - | chr5.3:23182950-23182969 | None:intergenic | 35.0% | |
| GCAATCACATTGTTGTGTAT+TGG | + | chr5.3:23180705-23180724 | MS.gene53478:intron | 35.0% | |
| TACCTTATTGGCAATGTTGT+TGG | - | chr5.3:23182445-23182464 | None:intergenic | 35.0% | |
| TACTACAGTGAAAGACAGAA+AGG | - | chr5.3:23181425-23181444 | None:intergenic | 35.0% | |
| TGCTTGTACAACTCTGATTT+GGG | + | chr5.3:23181775-23181794 | MS.gene53478:intron | 35.0% | |
| TTCCAACAACATTGCCAATA+AGG | + | chr5.3:23182440-23182459 | MS.gene53478:CDS | 35.0% | |
| TTGCTTGTACAACTCTGATT+TGG | + | chr5.3:23181774-23181793 | MS.gene53478:intron | 35.0% | |
| ! | ACTTAGTAGTCTTTTTGTGG+GGG | + | chr5.3:23182905-23182924 | MS.gene53478:intron | 35.0% |
| ! | AGAACATTAGCTTCACTGTT+TGG | + | chr5.3:23181310-23181329 | MS.gene53478:CDS | 35.0% |
| ! | ATTGGACACATGTTTCTGAA+TGG | + | chr5.3:23180723-23180742 | MS.gene53478:intron | 35.0% |
| ! | ATTGGGAAGATCTTGTTTGT+TGG | - | chr5.3:23182213-23182232 | None:intergenic | 35.0% |
| ! | CACTATAGGTTTTTCCTTGT+AGG | + | chr5.3:23180762-23180781 | MS.gene53478:intron | 35.0% |
| ! | CACTTAAATGATTTTGTCCC+AGG | + | chr5.3:23182503-23182522 | MS.gene53478:intron | 35.0% |
| ! | GAACATTAGCTTCACTGTTT+GGG | + | chr5.3:23181311-23181330 | MS.gene53478:CDS | 35.0% |
| AAACTTACCGATAGTAGGGA+TGG | - | chr5.3:23180548-23180567 | None:intergenic | 40.0% | |
| AAGCTTGAGCTTGTAGAGAA+TGG | - | chr5.3:23180509-23180528 | None:intergenic | 40.0% | |
| AATTTCTGTCGTGCTTCTCA+TGG | + | chr5.3:23181864-23181883 | MS.gene53478:intron | 40.0% | |
| ACAGTGAAAGACAGAAAGGA+GGG | - | chr5.3:23181421-23181440 | None:intergenic | 40.0% | |
| ATATAAGACCCTGAGTGTTC+TGG | - | chr5.3:23181505-23181524 | None:intergenic | 40.0% | |
| ATTCTCTACAAGCTCAAGCT+TGG | + | chr5.3:23180508-23180527 | MS.gene53478:CDS | 40.0% | |
| ATTTCTGTCGTGCTTCTCAT+GGG | + | chr5.3:23181865-23181884 | MS.gene53478:intron | 40.0% | |
| CATCTAGATGCCTAGAGTAT+GGG | - | chr5.3:23182831-23182850 | None:intergenic | 40.0% | |
| CCAAGAAACTTACCGATAGT+AGG | - | chr5.3:23180553-23180572 | None:intergenic | 40.0% | |
| CCTACTATCGGTAAGTTTCT+TGG | + | chr5.3:23180550-23180569 | MS.gene53478:intron | 40.0% | |
| CCTGCAATGATAGAAAACCA+GGG | - | chr5.3:23183091-23183110 | None:intergenic | 40.0% | |
| CTGTCTTTCACTGTAGTATG+TGG | + | chr5.3:23181427-23181446 | MS.gene53478:intron | 40.0% | |
| GAAATGCGAATTCTGATGGT+TGG | + | chr5.3:23180460-23180479 | MS.gene53478:CDS | 40.0% | |
| GAAGGAAATGCGAATTCTGA+TGG | + | chr5.3:23180456-23180475 | MS.gene53478:CDS | 40.0% | |
| GCTACATAGGATGTTGAATG+AGG | + | chr5.3:23181574-23181593 | MS.gene53478:CDS | 40.0% | |
| TACAGTGAAAGACAGAAAGG+AGG | - | chr5.3:23181422-23181441 | None:intergenic | 40.0% | |
| TATGTTTAGGTGCTCAGAAG+GGG | - | chr5.3:23182937-23182956 | None:intergenic | 40.0% | |
| TCAAATGAGAAGCTCTCGTA+AGG | - | chr5.3:23181021-23181040 | None:intergenic | 40.0% | |
| TCAGCTGCATTCATTGCATT+GGG | - | chr5.3:23182230-23182249 | None:intergenic | 40.0% | |
| TGTGTTCTAACAAACGCTTC+CGG | + | chr5.3:23182348-23182367 | MS.gene53478:intron | 40.0% | |
| ! | AGCGTTTGTTAGAACACAGT+AGG | - | chr5.3:23182346-23182365 | None:intergenic | 40.0% |
| ! | GACACATGTTTCTGAATGGT+CGG | + | chr5.3:23180727-23180746 | MS.gene53478:intron | 40.0% |
| ! | GGTGCTATGTAGTTCACTAT+AGG | + | chr5.3:23180748-23180767 | MS.gene53478:intron | 40.0% |
| ACAAGTGCTCTGTATGTACC+TGG | - | chr5.3:23182379-23182398 | None:intergenic | 45.0% | |
| AGCTGAGATTACTGACAAGC+TGG | + | chr5.3:23182244-23182263 | MS.gene53478:CDS | 45.0% | |
| AGTCTCACTAGTCCCAAATC+AGG | - | chr5.3:23182575-23182594 | None:intergenic | 45.0% | |
| AGTGAAAGACAGAAAGGAGG+GGG | - | chr5.3:23181419-23181438 | None:intergenic | 45.0% | |
| ATGTTTAGGTGCTCAGAAGG+GGG | - | chr5.3:23182936-23182955 | None:intergenic | 45.0% | |
| CACTACTTCCAGAACACTCA+GGG | + | chr5.3:23181494-23181513 | MS.gene53478:CDS | 45.0% | |
| CACTCATATAGACCGTGTAG+TGG | + | chr5.3:23181674-23181693 | MS.gene53478:intron | 45.0% | |
| CAGTCGGTTATTTGCGAAGA+AGG | + | chr5.3:23180438-23180457 | MS.gene53478:CDS | 45.0% | |
| CAGTGAAAGACAGAAAGGAG+GGG | - | chr5.3:23181420-23181439 | None:intergenic | 45.0% | |
| CATCCTATGTAGCTCATCTC+TGG | - | chr5.3:23181567-23181586 | None:intergenic | 45.0% | |
| CATTCACGAAGCTGTTCAGT+CGG | + | chr5.3:23180422-23180441 | MS.gene53478:CDS | 45.0% | |
| CTCAGCTGCATTCATTGCAT+TGG | - | chr5.3:23182231-23182250 | None:intergenic | 45.0% | |
| CTTGAGCTTGTAGAGAATGG+TGG | - | chr5.3:23180506-23180525 | None:intergenic | 45.0% | |
| GAGGTCGCTTATTATTTCCC+TGG | + | chr5.3:23183071-23183090 | MS.gene53478:intron | 45.0% | |
| GCATCTAGATGCCTAGAGTA+TGG | - | chr5.3:23182832-23182851 | None:intergenic | 45.0% | |
| GCCTGCAATGATAGAAAACC+AGG | - | chr5.3:23183092-23183111 | None:intergenic | 45.0% | |
| GCTGAGATTACTGACAAGCT+GGG | + | chr5.3:23182245-23182264 | MS.gene53478:CDS | 45.0% | |
| GTCTCACTAGTCCCAAATCA+GGG | - | chr5.3:23182574-23182593 | None:intergenic | 45.0% | |
| GTCTTTATGAAGGTCTGGAC+TGG | + | chr5.3:23182415-23182434 | MS.gene53478:CDS | 45.0% | |
| GTGTTCTAACAAACGCTTCC+GGG | + | chr5.3:23182349-23182368 | MS.gene53478:intron | 45.0% | |
| TAAGCTCACACCCATACTCT+AGG | + | chr5.3:23182818-23182837 | MS.gene53478:intron | 45.0% | |
| TAGCCAAGAACTAAGAAGGC+TGG | - | chr5.3:23183032-23183051 | None:intergenic | 45.0% | |
| TCATATAGACCGTGTAGTGG+CGG | + | chr5.3:23181677-23181696 | MS.gene53478:intron | 45.0% | |
| TGACAGGGATAGAGTTGTTG+AGG | + | chr5.3:23181541-23181560 | MS.gene53478:CDS | 45.0% | |
| TGTCGTTGACAGCAATGACA+GGG | + | chr5.3:23181526-23181545 | MS.gene53478:CDS | 45.0% | |
| TTGTCGTTGACAGCAATGAC+AGG | + | chr5.3:23181525-23181544 | MS.gene53478:CDS | 45.0% | |
| ! | AGCTTCACTGTTTGGGATGT+CGG | + | chr5.3:23181318-23181337 | MS.gene53478:CDS | 45.0% |
| ! | AGTGCTTTTAGGCCCTGATT+TGG | + | chr5.3:23182559-23182578 | MS.gene53478:intron | 45.0% |
| ! | GTGCTTTTAGGCCCTGATTT+GGG | + | chr5.3:23182560-23182579 | MS.gene53478:intron | 45.0% |
| ! | TTGCCAGCCTTCTTAGTTCT+TGG | + | chr5.3:23183026-23183045 | MS.gene53478:intron | 45.0% |
| !! | CCCTGGTTTTCTATCATTGC+AGG | + | chr5.3:23183088-23183107 | MS.gene53478:intron | 45.0% |
| !! | TGAAATTATAATTAAAATAA+TGG | - | chr5.3:23182155-23182174 | None:intergenic | 5.0% |
| !!! | ATTATTTTAATTATAATTTC+AGG | + | chr5.3:23182154-23182173 | MS.gene53478:intron | 5.0% |
| !!! | ATTTTTTTTCAAATAATTAT+CGG | - | chr5.3:23180672-23180691 | None:intergenic | 5.0% |
| AGGCCAGAGATGAGCTACAT+AGG | + | chr5.3:23181561-23181580 | MS.gene53478:CDS | 50.0% | |
| AGGGTTCAATGTGGAGACTG+TGG | + | chr5.3:23181281-23181300 | MS.gene53478:intron | 50.0% | |
| CATATAGACCGTGTAGTGGC+GGG | + | chr5.3:23181678-23181697 | MS.gene53478:intron | 50.0% | |
| CTGTATGTACCTGGAAGACC+CGG | - | chr5.3:23182370-23182389 | None:intergenic | 50.0% | |
| CTTACCGATAGTAGGGATGG+TGG | - | chr5.3:23180545-23180564 | None:intergenic | 50.0% | |
| GCACTACTTCCAGAACACTC+AGG | + | chr5.3:23181493-23181512 | MS.gene53478:CDS | 50.0% | |
| GGAGGGTCTTTATGAAGGTC+TGG | + | chr5.3:23182410-23182429 | MS.gene53478:CDS | 50.0% | |
| GTCACCACCATCCCTACTAT+CGG | + | chr5.3:23180538-23180557 | MS.gene53478:CDS | 50.0% | |
| GTGAAAGACAGAAAGGAGGG+GGG | - | chr5.3:23181418-23181437 | None:intergenic | 50.0% | |
| TCTGGGGAGGGTCTTTATGA+AGG | + | chr5.3:23182405-23182424 | MS.gene53478:CDS | 50.0% | |
| TGAAAGACAGAAAGGAGGGG+GGG | - | chr5.3:23181417-23181436 | None:intergenic | 50.0% | |
| TGGAAGTAGTGCCTCCACAA+GGG | - | chr5.3:23181485-23181504 | None:intergenic | 50.0% | |
| TTCATAAAGACCCTCCCCAG+AGG | - | chr5.3:23182406-23182425 | None:intergenic | 50.0% | |
| ! | AAGAGAAGAGCATGGACTCG+AGG | - | chr5.3:23182101-23182120 | None:intergenic | 50.0% |
| ! | TTCACTGTTTGGGATGTCGG+AGG | + | chr5.3:23181321-23181340 | MS.gene53478:CDS | 50.0% |
| !! | TGCAAGAGTGCTTCGCAGAA+AGG | + | chr5.3:23181202-23181221 | MS.gene53478:intron | 50.0% |
| AGTAGTGCCTCCACAAGGGA+CGG | - | chr5.3:23181481-23181500 | None:intergenic | 55.0% | |
| ATAAAGACCCTCCCCAGAGG+TGG | - | chr5.3:23182403-23182422 | None:intergenic | 55.0% | |
| CATTCCTTCCCGCCACTACA+CGG | - | chr5.3:23181689-23181708 | None:intergenic | 55.0% | |
| CTGGAAGTAGTGCCTCCACA+AGG | - | chr5.3:23181486-23181505 | None:intergenic | 55.0% | |
| TTCTCCAGATCCGTCCCTTG+TGG | + | chr5.3:23181468-23181487 | MS.gene53478:intron | 55.0% | |
| ! | AGAGCACTTGTGCCACCTCT+GGG | + | chr5.3:23182388-23182407 | MS.gene53478:CDS | 55.0% |
| !! | ATGGTTGGTCTCGATGCAGC+TGG | + | chr5.3:23180475-23180494 | MS.gene53478:CDS | 55.0% |
| ACTCTCTCAGACAGCGCCAC+TGG | + | chr5.3:23182273-23182292 | MS.gene53478:CDS | 60.0% | |
| CAAACGCTTCCGGGTCTTCC+AGG | + | chr5.3:23182358-23182377 | MS.gene53478:intron | 60.0% | |
| GAGCACTTGTGCCACCTCTG+GGG | + | chr5.3:23182389-23182408 | MS.gene53478:CDS | 60.0% | |
| TCCAGATCCGTCCCTTGTGG+AGG | + | chr5.3:23181471-23181490 | MS.gene53478:intron | 60.0% | |
| ! | ACTTGTGCCACCTCTGGGGA+GGG | + | chr5.3:23182393-23182412 | MS.gene53478:CDS | 60.0% |
| ! | CAGAGCACTTGTGCCACCTC+TGG | + | chr5.3:23182387-23182406 | MS.gene53478:CDS | 60.0% |
| ! | TAGACCGTGTAGTGGCGGGA+AGG | + | chr5.3:23181682-23181701 | MS.gene53478:intron | 60.0% |
| ! | TGTTTGGGATGTCGGAGGCC+AGG | + | chr5.3:23181326-23181345 | MS.gene53478:CDS | 60.0% |
| GCCTCCACAAGGGACGGATC+TGG | - | chr5.3:23181475-23181494 | None:intergenic | 65.0% | |
| GGATGTCGGAGGCCAGGACA+AGG | + | chr5.3:23181332-23181351 | MS.gene53478:CDS | 65.0% | |
| ! | CACTTGTGCCACCTCTGGGG+AGG | + | chr5.3:23182392-23182411 | MS.gene53478:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 23180412 | 23183115 | 23180412 | ID=MS.gene53478 |
| chr5.3 | mRNA | 23180412 | 23183115 | 23180412 | ID=MS.gene53478.t1;Parent=MS.gene53478 |
| chr5.3 | exon | 23180412 | 23180559 | 23180412 | ID=MS.gene53478.t1.exon1;Parent=MS.gene53478.t1 |
| chr5.3 | CDS | 23180412 | 23180559 | 23180412 | ID=cds.MS.gene53478.t1;Parent=MS.gene53478.t1 |
| chr5.3 | exon | 23181283 | 23181353 | 23181283 | ID=MS.gene53478.t1.exon2;Parent=MS.gene53478.t1 |
| chr5.3 | CDS | 23181283 | 23181353 | 23181283 | ID=cds.MS.gene53478.t1;Parent=MS.gene53478.t1 |
| chr5.3 | exon | 23181476 | 23181595 | 23181476 | ID=MS.gene53478.t1.exon3;Parent=MS.gene53478.t1 |
| chr5.3 | CDS | 23181476 | 23181595 | 23181476 | ID=cds.MS.gene53478.t1;Parent=MS.gene53478.t1 |
| chr5.3 | exon | 23182176 | 23182294 | 23182176 | ID=MS.gene53478.t1.exon4;Parent=MS.gene53478.t1 |
| chr5.3 | CDS | 23182176 | 23182294 | 23182176 | ID=cds.MS.gene53478.t1;Parent=MS.gene53478.t1 |
| chr5.3 | exon | 23182380 | 23182461 | 23182380 | ID=MS.gene53478.t1.exon5;Parent=MS.gene53478.t1 |
| chr5.3 | CDS | 23182380 | 23182461 | 23182380 | ID=cds.MS.gene53478.t1;Parent=MS.gene53478.t1 |
| chr5.3 | exon | 23183110 | 23183115 | 23183110 | ID=MS.gene53478.t1.exon6;Parent=MS.gene53478.t1 |
| chr5.3 | CDS | 23183110 | 23183115 | 23183110 | ID=cds.MS.gene53478.t1;Parent=MS.gene53478.t1 |
| Gene Sequence |
| Protein sequence |
