Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027535.t1 | XP_003613366.2 | 89.1 | 265 | 29 | 0 | 2 | 266 | 168 | 432 | 2.20E-136 | 495 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027535.t1 | G7K479 | 89.1 | 265 | 29 | 0 | 2 | 266 | 123 | 387 | 1.6e-136 | 495.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene68296 | MS.gene027535 | PPI |
| MS.gene027535 | MS.gene027537 | PPI |
| MS.gene027535 | MS.gene027534 | PPI |
| MS.gene027535 | MS.gene68292 | PPI |
| MS.gene027535 | MS.gene68298 | PPI |
| MS.gene027535 | MS.gene027543 | PPI |
| MS.gene68297 | MS.gene027535 | PPI |
| MS.gene45115 | MS.gene027535 | PPI |
| MS.gene027535 | MS.gene43936 | PPI |
| MS.gene027535 | MS.gene45119 | PPI |
| MS.gene027535 | MS.gene43935 | PPI |
| MS.gene45117 | MS.gene027535 | PPI |
| MS.gene027534 | MS.gene027535 | PPI |
| MS.gene027535 | MS.gene053932 | PPI |
| MS.gene027537 | MS.gene027535 | PPI |
| MS.gene027535 | MS.gene78745 | PPI |
| MS.gene68295 | MS.gene027535 | PPI |
| MS.gene027535 | MS.gene027538 | PPI |
| MS.gene027535 | MS.gene027536 | PPI |
| MS.gene045965 | MS.gene027535 | PPI |
| MS.gene027535 | MS.gene45120 | PPI |
| MS.gene43935 | MS.gene027535 | PPI |
| MS.gene027544 | MS.gene027535 | PPI |
| MS.gene027535 | MS.gene45118 | PPI |
| MS.gene027535 | MS.gene027540 | PPI |
| MS.gene027535 | MS.gene042700 | PPI |
| MS.gene68293 | MS.gene027535 | PPI |
| MS.gene027535 | MS.gene45114 | PPI |
| MS.gene027535 | MS.gene027539 | PPI |
| MS.gene027535 | MS.gene45116 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027535.t1 | MTR_5g035750 | 89.057 | 265 | 29 | 0 | 2 | 266 | 123 | 387 | 6.88e-178 | 495 |
| MS.gene027535.t1 | MTR_5g035690 | 88.346 | 266 | 31 | 0 | 1 | 266 | 121 | 386 | 1.42e-176 | 491 |
| MS.gene027535.t1 | MTR_5g035665 | 87.925 | 265 | 32 | 0 | 2 | 266 | 115 | 379 | 3.87e-176 | 490 |
| MS.gene027535.t1 | MTR_5g035670 | 80.524 | 267 | 50 | 1 | 2 | 266 | 115 | 381 | 6.27e-157 | 441 |
| MS.gene027535.t1 | MTR_5g035760 | 78.358 | 268 | 56 | 2 | 1 | 266 | 113 | 380 | 2.57e-150 | 425 |
| MS.gene027535.t1 | MTR_6g472110 | 51.418 | 282 | 114 | 11 | 5 | 266 | 121 | 399 | 1.48e-80 | 248 |
| MS.gene027535.t1 | MTR_6g016660 | 42.908 | 282 | 137 | 8 | 6 | 266 | 120 | 398 | 7.18e-65 | 207 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027535.t1 | AT1G13520 | 44.043 | 277 | 130 | 10 | 4 | 266 | 122 | 387 | 5.06e-61 | 197 |
| MS.gene027535.t1 | AT1G13510 | 42.336 | 274 | 138 | 8 | 1 | 266 | 119 | 380 | 2.23e-60 | 196 |
| MS.gene027535.t1 | AT1G13480 | 41.935 | 279 | 139 | 9 | 1 | 266 | 119 | 387 | 6.72e-59 | 192 |
| MS.gene027535.t1 | AT1G13540 | 44.161 | 274 | 133 | 8 | 1 | 266 | 120 | 381 | 2.59e-58 | 190 |
| MS.gene027535.t1 | AT1G13490 | 42.960 | 277 | 136 | 9 | 2 | 266 | 116 | 382 | 3.03e-57 | 187 |
| MS.gene027535.t1 | AT1G13530 | 39.493 | 276 | 148 | 9 | 1 | 266 | 119 | 385 | 2.46e-56 | 185 |
| MS.gene027535.t1 | AT1G13490 | 42.963 | 270 | 132 | 9 | 9 | 266 | 104 | 363 | 3.18e-54 | 179 |
| MS.gene027535.t1 | AT1G13470 | 43.066 | 274 | 138 | 9 | 1 | 266 | 115 | 378 | 8.75e-54 | 179 |
| MS.gene027535.t1 | AT1G13500 | 41.367 | 278 | 141 | 9 | 2 | 266 | 118 | 386 | 1.13e-52 | 176 |
| MS.gene027535.t1 | AT1G61600 | 38.079 | 302 | 144 | 8 | 6 | 266 | 122 | 421 | 6.49e-51 | 172 |
| MS.gene027535.t1 | AT1G13550 | 37.545 | 277 | 149 | 9 | 1 | 263 | 119 | 385 | 1.23e-47 | 163 |
Find 54 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGAACAATCATTGAAAAT+TGG | 0.211604 | 5.4:+40733589 | None:intergenic |
| AAAATTGGAAAGTTGAAATT+TGG | 0.232410 | 5.4:+40733604 | None:intergenic |
| AGCCTAAAGCTTGGCTTAAA+TGG | 0.235862 | 5.4:+40733653 | None:intergenic |
| CTCTCCTTCACAAACATAAA+TGG | 0.257853 | 5.4:+40733538 | None:intergenic |
| GAAGGAGAGAATGAATTTAA+AGG | 0.260016 | 5.4:-40733524 | MS.gene027535:CDS |
| TCAGACACTCACATCAAATT+AGG | 0.281648 | 5.4:-40733087 | MS.gene027535:CDS |
| TTGAAGGGCTCTAAAGCTAT+TGG | 0.344879 | 5.4:+40733808 | None:intergenic |
| GCCTCAGATGGAATTCCTCC+TGG | 0.354070 | 5.4:-40733727 | MS.gene027535:CDS |
| CACATCAAATTAGGAGCAAA+TGG | 0.354134 | 5.4:-40733078 | MS.gene027535:CDS |
| TTGGCTTAAATGGTGGTTGC+GGG | 0.360622 | 5.4:+40733663 | None:intergenic |
| TGAAGGAAAGGACGCTGTTT+GGG | 0.368363 | 5.4:-40733371 | MS.gene027535:CDS |
| TTGAAGGAAAGGACGCTGTT+TGG | 0.369269 | 5.4:-40733372 | MS.gene027535:CDS |
| ACAAAAGTTGAAGAGTTTGA+TGG | 0.392275 | 5.4:-40733202 | MS.gene027535:CDS |
| AAATGGGAACAAAGAAGGTT+TGG | 0.394970 | 5.4:-40733259 | MS.gene027535:CDS |
| AGAAGGTTTGGATGGAATGC+TGG | 0.431581 | 5.4:-40733247 | MS.gene027535:CDS |
| AGCTAACACTTGAGCAAAGA+TGG | 0.431951 | 5.4:-40733474 | MS.gene027535:CDS |
| CACCATTTAAGCCAAGCTTT+AGG | 0.434225 | 5.4:-40733655 | MS.gene027535:CDS |
| GCCCTTCAATGATTATCAAC+AGG | 0.448229 | 5.4:-40733794 | MS.gene027535:CDS |
| ACAGAAGTTGCTAAGGTTGA+AGG | 0.453239 | 5.4:-40733388 | MS.gene027535:CDS |
| GATGAAAATAGATTGGTTGA+TGG | 0.465731 | 5.4:-40733349 | MS.gene027535:CDS |
| TACCTGTTGATAATCATTGA+AGG | 0.466990 | 5.4:+40733792 | None:intergenic |
| TTTGGATGGAATGCTGGGAA+TGG | 0.475064 | 5.4:-40733241 | MS.gene027535:CDS |
| GAATTCCTCCTGGGTTATTG+AGG | 0.475186 | 5.4:-40733717 | MS.gene027535:CDS |
| TCAAAGGAAAATAGTGGAGA+AGG | 0.489041 | 5.4:-40733439 | MS.gene027535:CDS |
| TTTCTATTGAAGAGAATGGA+TGG | 0.489855 | 5.4:-40733133 | MS.gene027535:CDS |
| TGTTTGGGATGAAAATAGAT+TGG | 0.492208 | 5.4:-40733356 | MS.gene027535:CDS |
| CTTGGCTTAAATGGTGGTTG+CGG | 0.507449 | 5.4:+40733662 | None:intergenic |
| GCAAAATCAGTTGCCTCAGA+TGG | 0.507539 | 5.4:-40733739 | MS.gene027535:CDS |
| CTGAGTTTGGAAGTTGTTGA+GGG | 0.521064 | 5.4:-40733286 | MS.gene027535:CDS |
| GGGAACAAAGAAGGTTTGGA+TGG | 0.521569 | 5.4:-40733255 | MS.gene027535:CDS |
| CCTCAGATGGAATTCCTCCT+GGG | 0.522046 | 5.4:-40733726 | MS.gene027535:CDS |
| TGTTCTGAATCAGTGGTTGT+TGG | 0.524665 | 5.4:-40733574 | MS.gene027535:CDS |
| ACATCAAATTAGGAGCAAAT+GGG | 0.536534 | 5.4:-40733077 | MS.gene027535:CDS |
| GAATGAAATGGGAACAAAGA+AGG | 0.541076 | 5.4:-40733264 | MS.gene027535:CDS |
| ATTTGGTAGCTTCGAACGCA+AGG | 0.544069 | 5.4:+40733621 | None:intergenic |
| TATTCAAACAGAAGTTGCTA+AGG | 0.545070 | 5.4:-40733395 | MS.gene027535:CDS |
| ACCTGTTGATAATCATTGAA+GGG | 0.546054 | 5.4:+40733793 | None:intergenic |
| AGTTGTTGAGGGAATGAAAT+GGG | 0.546947 | 5.4:-40733275 | MS.gene027535:CDS |
| CAATGATTGTTCTGAATCAG+TGG | 0.548989 | 5.4:-40733581 | MS.gene027535:CDS |
| TTGTCCATTTATGTTTGTGA+AGG | 0.558931 | 5.4:-40733542 | MS.gene027535:CDS |
| GAAGGTTTGGATGGAATGCT+GGG | 0.561735 | 5.4:-40733246 | MS.gene027535:CDS |
| AATCATTTGAGCCTAAAGCT+TGG | 0.566503 | 5.4:+40733644 | None:intergenic |
| AGTTTGATGGAACAAATAAG+TGG | 0.584146 | 5.4:-40733189 | MS.gene027535:CDS |
| CTAAAGCTTGGCTTAAATGG+TGG | 0.584507 | 5.4:+40733656 | None:intergenic |
| AAAAGGTGGAAACTTAAGGC+TGG | 0.590085 | 5.4:-40733691 | MS.gene027535:CDS |
| ACTGAGTTTGGAAGTTGTTG+AGG | 0.595211 | 5.4:-40733287 | MS.gene027535:CDS |
| AGTTGCTAAGGTTGAAGGAA+AGG | 0.599670 | 5.4:-40733383 | MS.gene027535:CDS |
| AAGGAAAATAGTGGAGAAGG+TGG | 0.602522 | 5.4:-40733436 | MS.gene027535:CDS |
| TGGCTTAAATGGTGGTTGCG+GGG | 0.603595 | 5.4:+40733664 | None:intergenic |
| CCCAGGAGGAATTCCATCTG+AGG | 0.605151 | 5.4:+40733726 | None:intergenic |
| AAGTTGTTGAGGGAATGAAA+TGG | 0.611433 | 5.4:-40733276 | MS.gene027535:CDS |
| GCTAACACTTGAGCAAAGAT+GGG | 0.612705 | 5.4:-40733473 | MS.gene027535:CDS |
| AATCAGTGGTTGTTGGAAAG+TGG | 0.623216 | 5.4:-40733567 | MS.gene027535:CDS |
| AAAACTACAGCAAAAGCATG+TGG | 0.702446 | 5.4:+40733846 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTGGAAAGTTGAAATT+TGG | + | chr5.4:40733321-40733340 | None:intergenic | 20.0% |
| !! | GAAATAATCAAAAAAGGTTA+TGG | - | chr5.4:40733153-40733172 | MS.gene027535:CDS | 20.0% |
| !!! | ATTTTTTCAAAGGAAAATAG+TGG | - | chr5.4:40733477-40733496 | MS.gene027535:CDS | 20.0% |
| ! | CAGGTAGAAATAATCAAAAA+AGG | - | chr5.4:40733147-40733166 | MS.gene027535:CDS | 25.0% |
| ! | TCAGAACAATCATTGAAAAT+TGG | + | chr5.4:40733336-40733355 | None:intergenic | 25.0% |
| ACAAAAGTTGAAGAGTTTGA+TGG | - | chr5.4:40733720-40733739 | MS.gene027535:CDS | 30.0% | |
| ACATCAAATTAGGAGCAAAT+GGG | - | chr5.4:40733845-40733864 | MS.gene027535:CDS | 30.0% | |
| AGTTTGATGGAACAAATAAG+TGG | - | chr5.4:40733733-40733752 | MS.gene027535:CDS | 30.0% | |
| GAAAAAAAGGTGGAAACTTA+AGG | - | chr5.4:40733227-40733246 | MS.gene027535:CDS | 30.0% | |
| GAAGGAGAGAATGAATTTAA+AGG | - | chr5.4:40733398-40733417 | MS.gene027535:CDS | 30.0% | |
| TATTCAAACAGAAGTTGCTA+AGG | - | chr5.4:40733527-40733546 | MS.gene027535:CDS | 30.0% | |
| TTGTCCATTTATGTTTGTGA+AGG | - | chr5.4:40733380-40733399 | MS.gene027535:CDS | 30.0% | |
| TTTCTATTGAAGAGAATGGA+TGG | - | chr5.4:40733789-40733808 | MS.gene027535:CDS | 30.0% | |
| ! | GAGTTTTCTATTGAAGAGAA+TGG | - | chr5.4:40733785-40733804 | MS.gene027535:CDS | 30.0% |
| ! | GATGAAAATAGATTGGTTGA+TGG | - | chr5.4:40733573-40733592 | MS.gene027535:CDS | 30.0% |
| ! | TGTTTGGGATGAAAATAGAT+TGG | - | chr5.4:40733566-40733585 | MS.gene027535:CDS | 30.0% |
| !! | ACCTGTTGATAATCATTGAA+GGG | + | chr5.4:40733132-40733151 | None:intergenic | 30.0% |
| !! | AGTGTTGATGAGAAAAATGT+TGG | - | chr5.4:40733612-40733631 | MS.gene027535:CDS | 30.0% |
| !! | TACCTGTTGATAATCATTGA+AGG | + | chr5.4:40733133-40733152 | None:intergenic | 30.0% |
| !!! | ATGGGAGAAGATTTTTTCAA+AGG | - | chr5.4:40733467-40733486 | MS.gene027535:CDS | 30.0% |
| AAATGGGAACAAAGAAGGTT+TGG | - | chr5.4:40733663-40733682 | MS.gene027535:CDS | 35.0% | |
| AAGTTGTTGAGGGAATGAAA+TGG | - | chr5.4:40733646-40733665 | MS.gene027535:CDS | 35.0% | |
| AATCATTTGAGCCTAAAGCT+TGG | + | chr5.4:40733281-40733300 | None:intergenic | 35.0% | |
| AGTTGTTGAGGGAATGAAAT+GGG | - | chr5.4:40733647-40733666 | MS.gene027535:CDS | 35.0% | |
| CACATCAAATTAGGAGCAAA+TGG | - | chr5.4:40733844-40733863 | MS.gene027535:CDS | 35.0% | |
| CTCTCCTTCACAAACATAAA+TGG | + | chr5.4:40733387-40733406 | None:intergenic | 35.0% | |
| CTGGGTTATTGAGGAAAAAA+AGG | - | chr5.4:40733214-40733233 | MS.gene027535:CDS | 35.0% | |
| GAAAAATGTTGGACTGAGTT+TGG | - | chr5.4:40733623-40733642 | MS.gene027535:CDS | 35.0% | |
| GAATGAAATGGGAACAAAGA+AGG | - | chr5.4:40733658-40733677 | MS.gene027535:CDS | 35.0% | |
| GGTTATTGAGGAAAAAAAGG+TGG | - | chr5.4:40733217-40733236 | MS.gene027535:CDS | 35.0% | |
| TCAGACACTCACATCAAATT+AGG | - | chr5.4:40733835-40733854 | MS.gene027535:CDS | 35.0% | |
| ! | AAAACTACAGCAAAAGCATG+TGG | + | chr5.4:40733079-40733098 | None:intergenic | 35.0% |
| ! | CAATGATTGTTCTGAATCAG+TGG | - | chr5.4:40733341-40733360 | MS.gene027535:CDS | 35.0% |
| ! | TCAAAGGAAAATAGTGGAGA+AGG | - | chr5.4:40733483-40733502 | MS.gene027535:CDS | 35.0% |
| !!! | CTTTTTTTCCTCAATAACCC+AGG | + | chr5.4:40733216-40733235 | None:intergenic | 35.0% |
| AAAAGGTGGAAACTTAAGGC+TGG | - | chr5.4:40733231-40733250 | MS.gene027535:CDS | 40.0% | |
| AATCAGTGGTTGTTGGAAAG+TGG | - | chr5.4:40733355-40733374 | MS.gene027535:CDS | 40.0% | |
| ACAGAAGTTGCTAAGGTTGA+AGG | - | chr5.4:40733534-40733553 | MS.gene027535:CDS | 40.0% | |
| ACTGAGTTTGGAAGTTGTTG+AGG | - | chr5.4:40733635-40733654 | MS.gene027535:CDS | 40.0% | |
| AGCCTAAAGCTTGGCTTAAA+TGG | + | chr5.4:40733272-40733291 | None:intergenic | 40.0% | |
| AGCTAACACTTGAGCAAAGA+TGG | - | chr5.4:40733448-40733467 | MS.gene027535:CDS | 40.0% | |
| AGTTGCTAAGGTTGAAGGAA+AGG | - | chr5.4:40733539-40733558 | MS.gene027535:CDS | 40.0% | |
| CACCATTTAAGCCAAGCTTT+AGG | - | chr5.4:40733267-40733286 | MS.gene027535:CDS | 40.0% | |
| CTAAAGCTTGGCTTAAATGG+TGG | + | chr5.4:40733269-40733288 | None:intergenic | 40.0% | |
| CTGAGTTTGGAAGTTGTTGA+GGG | - | chr5.4:40733636-40733655 | MS.gene027535:CDS | 40.0% | |
| GCCCTTCAATGATTATCAAC+AGG | - | chr5.4:40733128-40733147 | MS.gene027535:CDS | 40.0% | |
| GCTAACACTTGAGCAAAGAT+GGG | - | chr5.4:40733449-40733468 | MS.gene027535:CDS | 40.0% | |
| ! | AAGGAAAATAGTGGAGAAGG+TGG | - | chr5.4:40733486-40733505 | MS.gene027535:CDS | 40.0% |
| ! | TGTTCTGAATCAGTGGTTGT+TGG | - | chr5.4:40733348-40733367 | MS.gene027535:CDS | 40.0% |
| ! | TTTTTCCTCAATAACCCAGG+AGG | + | chr5.4:40733213-40733232 | None:intergenic | 40.0% |
| !! | TTGAAGGGCTCTAAAGCTAT+TGG | + | chr5.4:40733117-40733136 | None:intergenic | 40.0% |
| !!! | GATTGGTTGATGGAGTTTTG+TGG | - | chr5.4:40733583-40733602 | MS.gene027535:CDS | 40.0% |
| AGAAGGTTTGGATGGAATGC+TGG | - | chr5.4:40733675-40733694 | MS.gene027535:CDS | 45.0% | |
| CTTGGCTTAAATGGTGGTTG+CGG | + | chr5.4:40733263-40733282 | None:intergenic | 45.0% | |
| GAAGGTTTGGATGGAATGCT+GGG | - | chr5.4:40733676-40733695 | MS.gene027535:CDS | 45.0% | |
| GAATTCCTCCTGGGTTATTG+AGG | - | chr5.4:40733205-40733224 | MS.gene027535:CDS | 45.0% | |
| GCAAAATCAGTTGCCTCAGA+TGG | - | chr5.4:40733183-40733202 | MS.gene027535:CDS | 45.0% | |
| GGGAACAAAGAAGGTTTGGA+TGG | - | chr5.4:40733667-40733686 | MS.gene027535:CDS | 45.0% | |
| TGAAGGAAAGGACGCTGTTT+GGG | - | chr5.4:40733551-40733570 | MS.gene027535:CDS | 45.0% | |
| TTGAAGGAAAGGACGCTGTT+TGG | - | chr5.4:40733550-40733569 | MS.gene027535:CDS | 45.0% | |
| TTGGCTTAAATGGTGGTTGC+GGG | + | chr5.4:40733262-40733281 | None:intergenic | 45.0% | |
| TTTGGATGGAATGCTGGGAA+TGG | - | chr5.4:40733681-40733700 | MS.gene027535:CDS | 45.0% | |
| ! | ATTTGGTAGCTTCGAACGCA+AGG | + | chr5.4:40733304-40733323 | None:intergenic | 45.0% |
| CCTCAGATGGAATTCCTCCT+GGG | - | chr5.4:40733196-40733215 | MS.gene027535:CDS | 50.0% | |
| TGGCTTAAATGGTGGTTGCG+GGG | + | chr5.4:40733261-40733280 | None:intergenic | 50.0% | |
| CCCAGGAGGAATTCCATCTG+AGG | + | chr5.4:40733199-40733218 | None:intergenic | 55.0% | |
| GCCTCAGATGGAATTCCTCC+TGG | - | chr5.4:40733195-40733214 | MS.gene027535:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 40733072 | 40733872 | 40733072 | ID=MS.gene027535 |
| chr5.4 | mRNA | 40733072 | 40733872 | 40733072 | ID=MS.gene027535.t1;Parent=MS.gene027535 |
| chr5.4 | exon | 40733072 | 40733872 | 40733072 | ID=MS.gene027535.t1.exon1;Parent=MS.gene027535.t1 |
| chr5.4 | CDS | 40733072 | 40733872 | 40733072 | ID=cds.MS.gene027535.t1;Parent=MS.gene027535.t1 |
| Gene Sequence |
| Protein sequence |
