AlfalfaGEDB | MS.gene43936

M. sativa cultivar XinJiangDaYe / MS.gene43936

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43936.t1	RHN55134.1	93.9	213	13	0	1	213	151	363	2.10E-113	418.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43936.t1	G7K475	93.9	213	13	0	1	213	121	333	1.5e-113	418.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene68293	MS.gene43936	PPI
MS.gene43936	MS.gene042700	PPI
MS.gene43936	MS.gene68298	PPI
MS.gene027535	MS.gene43936	PPI
MS.gene45115	MS.gene43936	PPI
MS.gene027540	MS.gene43936	PPI
MS.gene68296	MS.gene43936	PPI
MS.gene43936	MS.gene45119	PPI
MS.gene68292	MS.gene43936	PPI
MS.gene027543	MS.gene43936	PPI
MS.gene43936	MS.gene053932	PPI
MS.gene68297	MS.gene43936	PPI
MS.gene68295	MS.gene43936	PPI
MS.gene43936	MS.gene027538	PPI
MS.gene43936	MS.gene027536	PPI
MS.gene43935	MS.gene43936	PPI
MS.gene45117	MS.gene43936	PPI
MS.gene45114	MS.gene43936	PPI
MS.gene43936	MS.gene45120	PPI
MS.gene027544	MS.gene43936	PPI
MS.gene027534	MS.gene43936	PPI
MS.gene43936	MS.gene45118	PPI
MS.gene027537	MS.gene43936	PPI
MS.gene45116	MS.gene43936	PPI
MS.gene68298	MS.gene43936	PPI
MS.gene43936	MS.gene78745	PPI
MS.gene045965	MS.gene43936	PPI
MS.gene027539	MS.gene43936	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43936.t1	MTR_5g035690	93.897	213	13	0	1	213	121	333	9.00e-148	416
MS.gene43936.t1	MTR_5g035665	89.623	212	22	0	2	213	115	326	3.47e-143	404
MS.gene43936.t1	MTR_5g035750	86.792	212	28	0	2	213	123	334	2.30e-135	385
MS.gene43936.t1	MTR_5g035670	81.308	214	38	1	2	213	115	328	2.93e-124	356
MS.gene43936.t1	MTR_5g035760	77.209	215	47	2	1	213	113	327	5.85e-116	335
MS.gene43936.t1	MTR_6g472110	49.558	226	92	10	4	210	120	342	1.21e-54	179
MS.gene43936.t1	MTR_6g016660	39.738	229	114	8	6	213	120	345	2.45e-41	144

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43936.t1	AT1G13510	44.393	214	100	8	4	211	122	322	5.04e-46	156
MS.gene43936.t1	AT1G13520	44.700	217	97	8	4	207	122	328	2.79e-42	147
MS.gene43936.t1	AT1G13530	41.589	214	108	7	1	206	119	323	6.88e-42	145
MS.gene43936.t1	AT1G13480	40.444	225	112	8	1	213	119	333	1.95e-41	144
MS.gene43936.t1	AT1G13490	45.116	215	99	7	2	206	116	321	5.72e-41	143
MS.gene43936.t1	AT1G13540	45.161	217	100	8	1	211	120	323	7.16e-41	143
MS.gene43936.t1	AT1G61600	38.197	233	113	6	6	209	122	352	2.03e-38	137
MS.gene43936.t1	AT1G13490	45.192	208	95	7	9	206	104	302	4.72e-38	135
MS.gene43936.t1	AT1G13500	41.743	218	105	9	2	207	118	325	4.82e-38	135
MS.gene43936.t1	AT1G13470	44.550	211	99	8	4	206	118	318	5.93e-38	135
MS.gene43936.t1	AT1G13550	40.000	220	107	10	1	206	119	327	5.03e-31	117

Find 50 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCTAATGCTTGGCTTAAA+TGG	0.196314	5.4:-25463008	None:intergenic
CCTGGCTTATTAAGGGAAAA+AGG	0.208156	5.4:+25462952	MS.gene43936:CDS
GAATTCCTCCTGGCTTATTA+AGG	0.210362	5.4:+25462944	MS.gene43936:CDS
CCTTCCTTCACAAACATAAA+TGG	0.218760	5.4:-25463123	None:intergenic
TTGAAGGAAAGGATGCTATT+TGG	0.225875	5.4:+25463289	MS.gene43936:CDS
GAAGGAAGGAATTAATTTAA+AGG	0.281181	5.4:+25463137	MS.gene43936:CDS
GCAAAATCCGTTGCCTTTGA+TGG	0.299145	5.4:+25462922	MS.gene43936:CDS
TGAAGGAAAGGATGCTATTT+GGG	0.302453	5.4:+25463290	MS.gene43936:CDS
AATCAGTAGTTGTTGGAAAA+TGG	0.313100	5.4:+25463094	MS.gene43936:CDS
GAAAGGTTTGGATGGATTGC+TGG	0.360515	5.4:+25463414	MS.gene43936:CDS
TTGAAGGGCTCCAAAGCTAT+TGG	0.369259	5.4:-25462853	None:intergenic
TTGGCTTAAATGGTAGTTGC+GGG	0.394226	5.4:-25462998	None:intergenic
GCCTTTGATGGAATTCCTCC+TGG	0.396546	5.4:+25462934	MS.gene43936:CDS
AATTCCTCCTGGCTTATTAA+GGG	0.397833	5.4:+25462945	MS.gene43936:CDS
TGATGTTAAACCAATAGCTT+TGG	0.399590	5.4:+25462843	MS.gene43936:CDS
ACGATTGGAAAGTTGAAATT+CGG	0.411346	5.4:-25463057	None:intergenic
GTTTGGATGGATTGCTGGGA+AGG	0.415810	5.4:+25463419	MS.gene43936:CDS
GATGAAAATAGATTAGTTGA+TGG	0.429291	5.4:+25463312	MS.gene43936:CDS
TACCATTTAAGCCAAGCATT+AGG	0.431877	5.4:+25463006	MS.gene43936:CDS
AGCTAACACTTGAGCAAAGA+TGG	0.431951	5.4:+25463187	MS.gene43936:CDS
AAAGTTGAAATTCGGTAGCT+TGG	0.433143	5.4:-25463049	None:intergenic
AAATGGGAACAAGAAAGGTT+TGG	0.437035	5.4:+25463402	MS.gene43936:CDS
GCCCTTCAATGATTATCAAC+AGG	0.448229	5.4:+25462867	MS.gene43936:CDS
TGCTCTGAATCAGTAGTTGT+TGG	0.458637	5.4:+25463087	MS.gene43936:CDS
TACCTGTTGATAATCATTGA+AGG	0.466990	5.4:-25462869	None:intergenic
ACAGAAGTCGCTAAGGTTGA+AGG	0.473616	5.4:+25463273	MS.gene43936:CDS
TCAAAGGAAAATAGTGGAGA+AGG	0.489041	5.4:+25463222	MS.gene43936:CDS
AATCATTTGAACCTAATGCT+TGG	0.490134	5.4:-25463017	None:intergenic
TTTGGATGGATTGCTGGGAA+GGG	0.490992	5.4:+25463420	MS.gene43936:CDS
CCATTTATGTTTGTGAAGGA+AGG	0.497881	5.4:+25463123	MS.gene43936:CDS
CAATGAAATGGGAACAAGAA+AGG	0.514439	5.4:+25463397	MS.gene43936:CDS
GGGAACAAGAAAGGTTTGGA+TGG	0.517021	5.4:+25463406	MS.gene43936:CDS
TCAGAGCAATCATTAACGAT+TGG	0.520159	5.4:-25463072	None:intergenic
AAAGGTTTGGATGGATTGCT+GGG	0.524617	5.4:+25463415	MS.gene43936:CDS
AAGTTGTTGAGGCAATGAAA+TGG	0.534687	5.4:+25463385	MS.gene43936:CDS
ACCTGTTGATAATCATTGAA+GGG	0.546054	5.4:-25462868	None:intergenic
ATTCGGTAGCTTGGAACGCA+AGG	0.554145	5.4:-25463040	None:intergenic
CTTGGCTTAAATGGTAGTTG+CGG	0.559548	5.4:-25462999	None:intergenic
GCCAGGAGGAATTCCATCAA+AGG	0.565396	5.4:-25462935	None:intergenic
GATTAGTTGATGGAGTATTG+TGG	0.572201	5.4:+25463322	MS.gene43936:CDS
AGTTGTTGAGGCAATGAAAT+GGG	0.574296	5.4:+25463386	MS.gene43936:CDS
TTGTCCATTTATGTTTGTGA+AGG	0.585966	5.4:+25463119	MS.gene43936:CDS
TATTCAAACAGAAGTCGCTA+AGG	0.601290	5.4:+25463266	MS.gene43936:CDS
AGTCGCTAAGGTTGAAGGAA+AGG	0.601966	5.4:+25463278	MS.gene43936:CDS
AAGGAAAATAGTGGAGAAGG+TGG	0.602522	5.4:+25463225	MS.gene43936:CDS
AGGAATTCCATCAAAGGCAA+CGG	0.610356	5.4:-25462929	None:intergenic
GCTAACACTTGAGCAAAGAT+GGG	0.612705	5.4:+25463188	MS.gene43936:CDS
ATTGAGTTTGGAAGTTGTTG+AGG	0.614856	5.4:+25463374	MS.gene43936:CDS
TGGCTTAAATGGTAGTTGCG+GGG	0.627549	5.4:-25462997	None:intergenic
AAAACTACAACAAAAGCATG+TGG	0.665724	5.4:-25462815	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAAATAATCAAAAAAGGTTA+TGG	+	chr5.4:25462892-25462911	MS.gene43936:CDS	20.0%
!!!	ATTTTTTCAAAGGAAAATAG+TGG	+	chr5.4:25463216-25463235	MS.gene43936:CDS	20.0%
!	CAGGTAGAAATAATCAAAAA+AGG	+	chr5.4:25462886-25462905	MS.gene43936:CDS	25.0%
!	GAAGGAAGGAATTAATTTAA+AGG	+	chr5.4:25463137-25463156	MS.gene43936:CDS	25.0%
!!	GATGAAAATAGATTAGTTGA+TGG	+	chr5.4:25463312-25463331	MS.gene43936:CDS	25.0%
	AATCAGTAGTTGTTGGAAAA+TGG	+	chr5.4:25463094-25463113	MS.gene43936:CDS	30.0%
	AATCATTTGAACCTAATGCT+TGG	-	chr5.4:25463020-25463039	None:intergenic	30.0%
	ACGATTGGAAAGTTGAAATT+CGG	-	chr5.4:25463060-25463079	None:intergenic	30.0%
	GAAAAATGTTGGATTGAGTT+TGG	+	chr5.4:25463362-25463381	MS.gene43936:CDS	30.0%
	TGATGTTAAACCAATAGCTT+TGG	+	chr5.4:25462843-25462862	MS.gene43936:CDS	30.0%
	TTGTCCATTTATGTTTGTGA+AGG	+	chr5.4:25463119-25463138	MS.gene43936:CDS	30.0%
!	AAAACTACAACAAAAGCATG+TGG	-	chr5.4:25462818-25462837	None:intergenic	30.0%
!!	ACCTGTTGATAATCATTGAA+GGG	-	chr5.4:25462871-25462890	None:intergenic	30.0%
!!	AGTGTTGATGAGAAAAATGT+TGG	+	chr5.4:25463351-25463370	MS.gene43936:CDS	30.0%
!!	TACCTGTTGATAATCATTGA+AGG	-	chr5.4:25462872-25462891	None:intergenic	30.0%
!!!	ATGGGAGATGATTTTTTCAA+AGG	+	chr5.4:25463206-25463225	MS.gene43936:CDS	30.0%
	AAAGTTGAAATTCGGTAGCT+TGG	-	chr5.4:25463052-25463071	None:intergenic	35.0%
	AAATGGGAACAAGAAAGGTT+TGG	+	chr5.4:25463402-25463421	MS.gene43936:CDS	35.0%
	AACCTAATGCTTGGCTTAAA+TGG	-	chr5.4:25463011-25463030	None:intergenic	35.0%
	AAGTTGTTGAGGCAATGAAA+TGG	+	chr5.4:25463385-25463404	MS.gene43936:CDS	35.0%
	AATTCCTCCTGGCTTATTAA+GGG	+	chr5.4:25462945-25462964	MS.gene43936:CDS	35.0%
	AGTTGTTGAGGCAATGAAAT+GGG	+	chr5.4:25463386-25463405	MS.gene43936:CDS	35.0%
	ATTGAGTTTGGAAGTTGTTG+AGG	+	chr5.4:25463374-25463393	MS.gene43936:CDS	35.0%
	CAATGAAATGGGAACAAGAA+AGG	+	chr5.4:25463397-25463416	MS.gene43936:CDS	35.0%
	CCATTTATGTTTGTGAAGGA+AGG	+	chr5.4:25463123-25463142	MS.gene43936:CDS	35.0%
	CCTTCCTTCACAAACATAAA+TGG	-	chr5.4:25463126-25463145	None:intergenic	35.0%
	CTGGCTTATTAAGGGAAAAA+GGG	+	chr5.4:25462953-25462972	MS.gene43936:CDS	35.0%
	TACCATTTAAGCCAAGCATT+AGG	+	chr5.4:25463006-25463025	MS.gene43936:CDS	35.0%
	TATTCAAACAGAAGTCGCTA+AGG	+	chr5.4:25463266-25463285	MS.gene43936:CDS	35.0%
	TCAGAGCAATCATTAACGAT+TGG	-	chr5.4:25463075-25463094	None:intergenic	35.0%
	TTGAAGGAAAGGATGCTATT+TGG	+	chr5.4:25463289-25463308	MS.gene43936:CDS	35.0%
!	TCAAAGGAAAATAGTGGAGA+AGG	+	chr5.4:25463222-25463241	MS.gene43936:CDS	35.0%
!	TGAAGGAAAGGATGCTATTT+GGG	+	chr5.4:25463290-25463309	MS.gene43936:CDS	35.0%
!!	GATTAGTTGATGGAGTATTG+TGG	+	chr5.4:25463322-25463341	MS.gene43936:CDS	35.0%
	AGCTAACACTTGAGCAAAGA+TGG	+	chr5.4:25463187-25463206	MS.gene43936:CDS	40.0%
	AGGAATTCCATCAAAGGCAA+CGG	-	chr5.4:25462932-25462951	None:intergenic	40.0%
	CCTGGCTTATTAAGGGAAAA+AGG	+	chr5.4:25462952-25462971	MS.gene43936:CDS	40.0%
	CTTGGCTTAAATGGTAGTTG+CGG	-	chr5.4:25463002-25463021	None:intergenic	40.0%
	GAATTCCTCCTGGCTTATTA+AGG	+	chr5.4:25462944-25462963	MS.gene43936:CDS	40.0%
	GCCCTTCAATGATTATCAAC+AGG	+	chr5.4:25462867-25462886	MS.gene43936:CDS	40.0%
	GCTAACACTTGAGCAAAGAT+GGG	+	chr5.4:25463188-25463207	MS.gene43936:CDS	40.0%
	GCTTATTAAGGGAAAAAGGG+TGG	+	chr5.4:25462956-25462975	MS.gene43936:CDS	40.0%
	TTGGCTTAAATGGTAGTTGC+GGG	-	chr5.4:25463001-25463020	None:intergenic	40.0%
!	AAGGAAAATAGTGGAGAAGG+TGG	+	chr5.4:25463225-25463244	MS.gene43936:CDS	40.0%
!	CCTTTTTCCCTTAATAAGCC+AGG	-	chr5.4:25462955-25462974	None:intergenic	40.0%
!	TGCTCTGAATCAGTAGTTGT+TGG	+	chr5.4:25463087-25463106	MS.gene43936:CDS	40.0%
!	TTTTCCCTTAATAAGCCAGG+AGG	-	chr5.4:25462952-25462971	None:intergenic	40.0%
!!	AAAGGTTTGGATGGATTGCT+GGG	+	chr5.4:25463415-25463434	MS.gene43936:CDS	40.0%
	ACAGAAGTCGCTAAGGTTGA+AGG	+	chr5.4:25463273-25463292	MS.gene43936:CDS	45.0%
	AGTCGCTAAGGTTGAAGGAA+AGG	+	chr5.4:25463278-25463297	MS.gene43936:CDS	45.0%
	GCAAAATCCGTTGCCTTTGA+TGG	+	chr5.4:25462922-25462941	MS.gene43936:CDS	45.0%
	GGGAACAAGAAAGGTTTGGA+TGG	+	chr5.4:25463406-25463425	MS.gene43936:CDS	45.0%
	TGGCTTAAATGGTAGTTGCG+GGG	-	chr5.4:25463000-25463019	None:intergenic	45.0%
	TTGAAGGGCTCCAAAGCTAT+TGG	-	chr5.4:25462856-25462875	None:intergenic	45.0%
!!	GAAAGGTTTGGATGGATTGC+TGG	+	chr5.4:25463414-25463433	MS.gene43936:CDS	45.0%
!!	TTTGGATGGATTGCTGGGAA+GGG	+	chr5.4:25463420-25463439	MS.gene43936:CDS	45.0%
	ATTCGGTAGCTTGGAACGCA+AGG	-	chr5.4:25463043-25463062	None:intergenic	50.0%
	GCCAGGAGGAATTCCATCAA+AGG	-	chr5.4:25462938-25462957	None:intergenic	50.0%
	GCCTTTGATGGAATTCCTCC+TGG	+	chr5.4:25462934-25462953	MS.gene43936:CDS	50.0%
!!	GTTTGGATGGATTGCTGGGA+AGG	+	chr5.4:25463419-25463438	MS.gene43936:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	25462811	25463449	25462811	ID=MS.gene43936
chr5.4	mRNA	25462811	25463449	25462811	ID=MS.gene43936.t1;Parent=MS.gene43936
chr5.4	exon	25462811	25463449	25462811	ID=MS.gene43936.t1.exon1;Parent=MS.gene43936.t1
chr5.4	CDS	25462811	25463449	25462811	ID=cds.MS.gene43936.t1;Parent=MS.gene43936.t1

Gene Sequence

>MS.gene43936

ATGACCACATGCTTTTGTTGTAGTTTTGTCAATGATGTTAAACCAATAGCTTTGGAGCCCTTCAATGATTATCAACAGGTAGAAATAATCAAAAAAGGTTATGGTTTTCACGCAAAATCCGTTGCCTTTGATGGAATTCCTCCTGGCTTATTAAGGGAAAAAGGGTGGACACTTTATGCTAGCACTCCCCGCAACTACCATTTAAGCCAAGCATTAGGTTCAAATGATTCCTTGCGTTCCAAGCTACCGAATTTCAACTTTCCAATCGTTAATGATTGCTCTGAATCAGTAGTTGTTGGAAAATGGTATTGTCCATTTATGTTTGTGAAGGAAGGAATTAATTTAAAGGAGCAGATGAAGATGTCAGTGTTCTATGAGCTAACACTTGAGCAAAGATGGGAGATGATTTTTTCAAAGGAAAATAGTGGAGAAGGTGGTGTGCTTGTAGATGTTGCTATTCAAACAGAAGTCGCTAAGGTTGAAGGAAAGGATGCTATTTGGGATGAAAATAGATTAGTTGATGGAGTATTGTGGTTTAAGAGTGTTGATGAGAAAAATGTTGGATTGAGTTTGGAAGTTGTTGAGGCAATGAAATGGGAACAAGAAAGGTTTGGATGGATTGCTGGGAAGGGAAGACAA

Protein sequence

>MS.gene43936.t1

MTTCFCCSFVNDVKPIALEPFNDYQQVEIIKKGYGFHAKSVAFDGIPPGLLREKGWTLYASTPRNYHLSQALGSNDSLRSKLPNFNFPIVNDCSESVVVGKWYCPFMFVKEGINLKEQMKMSVFYELTLEQRWEMIFSKENSGEGGVLVDVAIQTEVAKVEGKDAIWDENRLVDGVLWFKSVDEKNVGLSLEVVEAMKWEQERFGWIAGKGRQ