Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027536.t1 | XP_003613367.1 | 86.5 | 341 | 34 | 2 | 1 | 329 | 1 | 341 | 8.80E-167 | 596.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027536.t1 | G7K480 | 86.5 | 341 | 34 | 2 | 1 | 329 | 1 | 341 | 6.3e-167 | 596.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene027536 | MS.gene78745 | PPI |
| MS.gene027543 | MS.gene027536 | PPI |
| MS.gene45117 | MS.gene027536 | PPI |
| MS.gene027534 | MS.gene027536 | PPI |
| MS.gene027537 | MS.gene027536 | PPI |
| MS.gene027536 | MS.gene042700 | PPI |
| MS.gene68298 | MS.gene027536 | PPI |
| MS.gene68295 | MS.gene027536 | PPI |
| MS.gene045965 | MS.gene027536 | PPI |
| MS.gene43935 | MS.gene027536 | PPI |
| MS.gene45114 | MS.gene027536 | PPI |
| MS.gene027544 | MS.gene027536 | PPI |
| MS.gene45119 | MS.gene027536 | PPI |
| MS.gene45120 | MS.gene027536 | PPI |
| MS.gene45116 | MS.gene027536 | PPI |
| MS.gene68293 | MS.gene027536 | PPI |
| MS.gene027539 | MS.gene027536 | PPI |
| MS.gene027535 | MS.gene027536 | PPI |
| MS.gene027536 | MS.gene053932 | PPI |
| MS.gene027538 | MS.gene027536 | PPI |
| MS.gene43936 | MS.gene027536 | PPI |
| MS.gene45118 | MS.gene027536 | PPI |
| MS.gene68296 | MS.gene027536 | PPI |
| MS.gene68292 | MS.gene027536 | PPI |
| MS.gene68297 | MS.gene027536 | PPI |
| MS.gene45115 | MS.gene027536 | PPI |
| MS.gene053932 | MS.gene027536 | PPI |
| MS.gene027540 | MS.gene027536 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027536.t1 | MTR_5g035760 | 86.510 | 341 | 34 | 2 | 1 | 329 | 1 | 341 | 0.0 | 604 |
| MS.gene027536.t1 | MTR_5g035665 | 72.515 | 342 | 79 | 3 | 1 | 329 | 1 | 340 | 1.35e-176 | 494 |
| MS.gene027536.t1 | MTR_5g035690 | 71.633 | 349 | 77 | 3 | 1 | 329 | 1 | 347 | 1.11e-174 | 489 |
| MS.gene027536.t1 | MTR_5g035750 | 68.857 | 350 | 86 | 4 | 1 | 329 | 1 | 348 | 5.83e-167 | 470 |
| MS.gene027536.t1 | MTR_5g035670 | 70.554 | 343 | 86 | 3 | 1 | 329 | 1 | 342 | 2.59e-165 | 466 |
| MS.gene027536.t1 | MTR_6g472110 | 44.847 | 359 | 168 | 7 | 1 | 329 | 1 | 359 | 1.86e-89 | 273 |
| MS.gene027536.t1 | MTR_6g016660 | 36.512 | 367 | 185 | 11 | 1 | 328 | 1 | 358 | 2.05e-59 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027536.t1 | AT1G13510 | 41.379 | 348 | 166 | 13 | 1 | 323 | 1 | 335 | 9.85e-70 | 222 |
| MS.gene027536.t1 | AT1G13530 | 41.761 | 352 | 169 | 11 | 1 | 326 | 1 | 342 | 1.42e-65 | 211 |
| MS.gene027536.t1 | AT1G13550 | 39.326 | 356 | 175 | 10 | 1 | 326 | 1 | 345 | 2.10e-65 | 211 |
| MS.gene027536.t1 | AT1G13490 | 41.954 | 348 | 171 | 11 | 1 | 326 | 1 | 339 | 3.55e-65 | 210 |
| MS.gene027536.t1 | AT1G13540 | 41.261 | 349 | 166 | 13 | 1 | 323 | 1 | 336 | 1.94e-64 | 208 |
| MS.gene027536.t1 | AT1G13520 | 38.983 | 354 | 178 | 11 | 1 | 326 | 1 | 344 | 1.36e-63 | 206 |
| MS.gene027536.t1 | AT1G13480 | 38.068 | 352 | 184 | 10 | 1 | 326 | 1 | 344 | 8.60e-63 | 204 |
| MS.gene027536.t1 | AT1G13470 | 38.746 | 351 | 180 | 10 | 1 | 328 | 1 | 339 | 5.94e-62 | 202 |
| MS.gene027536.t1 | AT1G61600 | 36.438 | 365 | 187 | 9 | 1 | 322 | 1 | 363 | 2.00e-61 | 202 |
| MS.gene027536.t1 | AT1G13490 | 40.634 | 347 | 158 | 11 | 1 | 326 | 1 | 320 | 4.26e-58 | 191 |
| MS.gene027536.t1 | AT1G13500 | 40.625 | 352 | 174 | 14 | 1 | 326 | 1 | 343 | 4.34e-56 | 187 |
Find 0 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATAGTAAATAATCAATA+AGG | + | 46418:769-788 | MS.gene027536:intergenic | 10.0% |
| !!! | AATTTTAAATATAATGTTCA+AGG | - | 46418:713-732 | MS.gene027536:CDS | 10.0% |
| !!! | AATAGATTAGTTATTATAAG+TGG | - | 46418:884-903 | MS.gene027536:intron | 15.0% |
| !!! | AATTTGTTGATAATGATTGA+AGG | + | 46418:1047-1066 | MS.gene027536:intergenic | 20.0% |
| !!! | ATATTTTTTTAATTCCGTGT+AGG | - | 46418:657-676 | MS.gene027536:CDS | 20.0% |
| !!! | ATTTGTTGATAATGATTGAA+GGG | + | 46418:1046-1065 | MS.gene027536:intergenic | 20.0% |
| ! | AAACTTAAATTCAAAGGAAG+AGG | - | 46418:961-980 | MS.gene027536:CDS | 25.0% |
| ! | AAGTTGTTAAAGGAATGAAA+TGG | - | 46418:1564-1583 | MS.gene027536:CDS | 25.0% |
| ! | AATGACATTAAAGGAACAAA+TGG | - | 46418:1319-1338 | MS.gene027536:intron | 25.0% |
| ! | AGTTATAATGTTCGATTCTA+AGG | + | 46418:745-764 | MS.gene027536:intergenic | 25.0% |
| ! | AGTTGTTAAAGGAATGAAAT+GGG | - | 46418:1565-1584 | MS.gene027536:CDS | 25.0% |
| ! | ATCCAATATTACAAGATAAC+CGG | + | 46418:402-421 | MS.gene027536:intergenic | 25.0% |
| ! | TATTATGTCATAAGAAGGAA+GGG | - | 46418:590-609 | MS.gene027536:CDS | 25.0% |
| ! | TCCTTCTTATGACATAATAA+CGG | + | 46418:589-608 | MS.gene027536:intergenic | 25.0% |
| ! | TTATTATGTCATAAGAAGGA+AGG | - | 46418:589-608 | MS.gene027536:CDS | 25.0% |
| !! | TTTTAATATCATGGCCAAAA+TGG | + | 46418:1009-1028 | MS.gene027536:intergenic | 25.0% |
| AAAGTTGAAATCTGGTAGTT+TGG | + | 46418:1225-1244 | MS.gene027536:intergenic | 30.0% | |
| ACCGTTATTATGTCATAAGA+AGG | - | 46418:585-604 | MS.gene027536:CDS | 30.0% | |
| AGAAAATGTCATGTTTCTTC+CGG | - | 46418:538-557 | MS.gene027536:CDS | 30.0% | |
| ATGGTTAAATGTTTGTTCTG+AGG | + | 46418:491-510 | MS.gene027536:intergenic | 30.0% | |
| ATTACAAGATAACCGGAATT+TGG | + | 46418:395-414 | MS.gene027536:intergenic | 30.0% | |
| ATTATGTCATAAGAAGGAAG+GGG | - | 46418:591-610 | MS.gene027536:CDS | 30.0% | |
| CCAAGCAAACTTAAATTCAA+AGG | - | 46418:955-974 | MS.gene027536:CDS | 30.0% | |
| GAATTCCTTGTGGTTTATTA+GGG | - | 46418:1118-1137 | MS.gene027536:intron | 30.0% | |
| GTAACAAAACTTGAAGAGTT+TGG | - | 46418:1635-1654 | MS.gene027536:CDS | 30.0% | |
| TAATAACGGTTTGAAGACAA+TGG | + | 46418:575-594 | MS.gene027536:intergenic | 30.0% | |
| TATACCTTCAAAATCACTGA+TGG | + | 46418:510-529 | MS.gene027536:intergenic | 30.0% | |
| TATGTCTTAAGCACACAATA+AGG | + | 46418:810-829 | MS.gene027536:intergenic | 30.0% | |
| TATTCAAACAGAAGTTGCTA+AGG | - | 46418:1445-1464 | MS.gene027536:CDS | 30.0% | |
| TATTCGGGATGAAAATAGAT+TGG | - | 46418:1484-1503 | MS.gene027536:CDS | 30.0% | |
| TGATATTAAAACAAGTCCCT+TGG | - | 46418:1018-1037 | MS.gene027536:CDS | 30.0% | |
| TGGTTAAATGTTTGTTCTGA+GGG | + | 46418:490-509 | MS.gene027536:intergenic | 30.0% | |
| TTCCGGTTATCTTGTAATAT+TGG | - | 46418:397-416 | MS.gene027536:CDS | 30.0% | |
| TTGAGTTTGGAAGTTGTTAA+AGG | - | 46418:1554-1573 | MS.gene027536:CDS | 30.0% | |
| ! | AGACATATTTTTCACGTTCT+AGG | - | 46418:848-867 | MS.gene027536:intron | 30.0% |
| ! | AGTGTTGAAGATAAAAGTGT+TGG | - | 46418:1530-1549 | MS.gene027536:CDS | 30.0% |
| ! | ATACCATTTTCCAACAAGAA+CGG | + | 46418:1273-1292 | MS.gene027536:intergenic | 30.0% |
| ! | CCTTTGAATTTAAGTTTGCT+TGG | + | 46418:958-977 | MS.gene027536:intergenic | 30.0% |
| ! | GATGAAAATAGATTGGTTGA+TGG | - | 46418:1491-1510 | MS.gene027536:CDS | 30.0% |
| ! | TGAAATAAGCAGAAACGTTT+TGG | - | 46418:1066-1085 | MS.gene027536:intron | 30.0% |
| ! | TTAACCATCAGTGATTTTGA+AGG | - | 46418:503-522 | MS.gene027536:CDS | 30.0% |
| ! | TTGTCCTTTTATGTTTGTGA+AGG | - | 46418:1292-1311 | MS.gene027536:intron | 30.0% |
| ! | TTTTTCCCTAATAAACCACA+AGG | + | 46418:1126-1145 | MS.gene027536:intergenic | 30.0% |
| !! | GATAATGGAAAGTTGAAATC+TGG | + | 46418:1233-1252 | MS.gene027536:intergenic | 30.0% |
| !! | TAAAAGTGTTGGATTGAGTT+TGG | - | 46418:1541-1560 | MS.gene027536:CDS | 30.0% |
| !!! | AGGGACTTGTTTTAATATCA+TGG | + | 46418:1018-1037 | MS.gene027536:intergenic | 30.0% |
| AAATGGGAACAAGAAAGGTT+TGG | - | 46418:1581-1600 | MS.gene027536:CDS | 35.0% | |
| AATCCGTTCTTGTTGGAAAA+TGG | - | 46418:1267-1286 | MS.gene027536:intron | 35.0% | |
| ACAAAACTTGAAGAGTTTGG+TGG | - | 46418:1638-1657 | MS.gene027536:CDS | 35.0% | |
| CCTTCCTTCACAAACATAAA+AGG | + | 46418:1299-1318 | MS.gene027536:intergenic | 35.0% | |
| GAAGGAAGGAATGACATTAA+AGG | - | 46418:1310-1329 | MS.gene027536:intron | 35.0% | |
| GAATGAAATGGGAACAAGAA+AGG | - | 46418:1576-1595 | MS.gene027536:CDS | 35.0% | |
| GCTCAAACTTACTCTTTCTT+TGG | - | 46418:428-447 | MS.gene027536:CDS | 35.0% | |
| GGAATTCCTTGTGGTTTATT+AGG | - | 46418:1117-1136 | MS.gene027536:intron | 35.0% | |
| GGTTTATTAGGGAAAAAGTG+TGG | - | 46418:1129-1148 | MS.gene027536:intron | 35.0% | |
| TCAGAACGATCATTGGATAA+TGG | + | 46418:1248-1267 | MS.gene027536:intergenic | 35.0% | |
| ! | CCTTTTATGTTTGTGAAGGA+AGG | - | 46418:1296-1315 | MS.gene027536:intron | 35.0% |
| ! | GACAATGGTTGATTCAATAC+CGG | + | 46418:560-579 | MS.gene027536:intergenic | 35.0% |
| ! | TCACTCAAATGGTAGTTTGT+AGG | + | 46418:1173-1192 | MS.gene027536:intergenic | 35.0% |
| ! | TGAATTTAAGTTTGCTTGGC+TGG | + | 46418:954-973 | MS.gene027536:intergenic | 35.0% |
| !! | AAATGTTTGTTCTGAGGGAA+AGG | + | 46418:485-504 | MS.gene027536:intergenic | 35.0% |
| !! | CTTACTCTTTCTTTGGTTTG+TGG | - | 46418:435-454 | MS.gene027536:CDS | 35.0% |
| AAAGGTTTGGATGGAATGCT+GGG | - | 46418:1594-1613 | MS.gene027536:CDS | 40.0% | |
| ACAGAAGTTGCTAAGGTTGA+AGG | - | 46418:1452-1471 | MS.gene027536:CDS | 40.0% | |
| AGTTGCTAAGGTTGAAGGAA+AGG | - | 46418:1457-1476 | MS.gene027536:CDS | 40.0% | |
| ATAATGATTGAAGGGCTCCA+AGG | + | 46418:1038-1057 | MS.gene027536:intergenic | 40.0% | |
| CATCAATTGAGCTACCTACA+CGG | + | 46418:674-693 | MS.gene027536:intergenic | 40.0% | |
| TAATGATTGAAGGGCTCCAA+GGG | + | 46418:1037-1056 | MS.gene027536:intergenic | 40.0% | |
| TGTGGAAGGACATTGGTATT+GGG | - | 46418:453-472 | MS.gene027536:CDS | 40.0% | |
| TTGAAGGAAAGGACGCTATT+CGG | - | 46418:1468-1487 | MS.gene027536:CDS | 40.0% | |
| TTGTGGAAGGACATTGGTAT+TGG | - | 46418:452-471 | MS.gene027536:CDS | 40.0% | |
| ! | AACGGATTCAGAACGATCAT+TGG | + | 46418:1255-1274 | MS.gene027536:intergenic | 40.0% |
| ! | ACTAGGGTTCCTTTTGTACA+TGG | + | 46418:345-364 | MS.gene027536:intergenic | 40.0% |
| ! | CAAATGGTAGTTTGTAGGAG+TGG | + | 46418:1168-1187 | MS.gene027536:intergenic | 40.0% |
| ! | CAAGGAATCATTTGAAGCCA+AGG | + | 46418:1198-1217 | MS.gene027536:intergenic | 40.0% |
| ! | GGTTCCTTTTGTACATGGAA+AGG | + | 46418:340-359 | MS.gene027536:intergenic | 40.0% |
| !! | ACTAGTGTATGCTGCCATTT+TGG | - | 46418:992-1011 | MS.gene027536:CDS | 40.0% |
| !! | CTCTTTCTTTGGTTTGTGGA+AGG | - | 46418:439-458 | MS.gene027536:CDS | 40.0% |
| !! | TTTGGTTTGTGGAAGGACAT+TGG | - | 46418:446-465 | MS.gene027536:CDS | 40.0% |
| !!! | GATTGGTTGATGGAGTTTTG+TGG | - | 46418:1501-1520 | MS.gene027536:CDS | 40.0% |
| AAGCCAAGGCTTCACTCAAA+TGG | + | 46418:1184-1203 | MS.gene027536:intergenic | 45.0% | |
| AGAAGGAAGGGGAAGAATCA+AGG | - | 46418:602-621 | MS.gene027536:CDS | 45.0% | |
| CTACCATTTGAGTGAAGCCT+TGG | - | 46418:1178-1197 | MS.gene027536:intron | 45.0% | |
| GAAAGGTTTGGATGGAATGC+TGG | - | 46418:1593-1612 | MS.gene027536:CDS | 45.0% | |
| GAAGGAAGGGGAAGAATCAA+GGG | - | 46418:603-622 | MS.gene027536:CDS | 45.0% | |
| GGCTCCTTTCCATGTACAAA+AGG | - | 46418:333-352 | MS.gene027536:CDS | 45.0% | |
| GGGAACAAGAAAGGTTTGGA+TGG | - | 46418:1585-1604 | MS.gene027536:CDS | 45.0% | |
| TAACCGGAATTTGGACCTGA+TGG | + | 46418:386-405 | MS.gene027536:intergenic | 45.0% | |
| TGAAGGAAAGGACGCTATTC+GGG | - | 46418:1469-1488 | MS.gene027536:CDS | 45.0% | |
| TTTGGATGGAATGCTGGGAA+GGG | - | 46418:1599-1618 | MS.gene027536:CDS | 45.0% | |
| ! | ATCTGGTAGTTTGGAACGCA+AGG | + | 46418:1216-1235 | MS.gene027536:intergenic | 45.0% |
| ! | CGTTCTGAATCCGTTCTTGT+TGG | - | 46418:1260-1279 | MS.gene027536:intron | 45.0% |
| !! | GTGGATCTGAAAGAGCACTA+GGG | + | 46418:361-380 | MS.gene027536:intergenic | 45.0% |
| AGTTGACGCTCGAGCAAAGA+TGG | - | 46418:1360-1379 | MS.gene027536:intron | 50.0% | |
| CCACCATCAGGTCCAAATTC+CGG | - | 46418:380-399 | MS.gene027536:CDS | 50.0% | |
| CTTTCAGATCCACCACCATC+AGG | - | 46418:368-387 | MS.gene027536:CDS | 50.0% | |
| GACTCGGATGGAATTCCTTG+TGG | - | 46418:1108-1127 | MS.gene027536:intron | 50.0% | |
| GTTGACGCTCGAGCAAAGAT+GGG | - | 46418:1361-1380 | MS.gene027536:intron | 50.0% | |
| GTTTGGATGGAATGCTGGGA+AGG | - | 46418:1598-1617 | MS.gene027536:CDS | 50.0% | |
| TCATGCCGAGTCAGTTGACT+CGG | - | 46418:1092-1111 | MS.gene027536:intron | 50.0% | |
| TCCATCCGAGTCAACTGACT+CGG | + | 46418:1100-1119 | MS.gene027536:intergenic | 50.0% | |
| !! | GAATTTGGACCTGATGGTGG+TGG | + | 46418:380-399 | MS.gene027536:intergenic | 50.0% |
| !! | GGTGGATCTGAAAGAGCACT+AGG | + | 46418:362-381 | MS.gene027536:intergenic | 50.0% |
| CCGGAATTTGGACCTGATGG+TGG | + | 46418:383-402 | MS.gene027536:intergenic | 55.0% | |
| GAATGCTGGGAAGGGAAGAC+AGG | - | 46418:1607-1626 | MS.gene027536:CDS | 55.0% | |
| !! | GCCGAGTCAGTTGACTCGGA+TGG | - | 46418:1096-1115 | MS.gene027536:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 46418 | gene | 320 | 1673 | 320 | ID=MS.gene027536 |
| 46418 | mRNA | 320 | 1673 | 320 | ID=MS.gene027536.t1;Parent=MS.gene027536 |
| 46418 | exon | 1369 | 1673 | 1369 | ID=MS.gene027536.t1.exon1;Parent=MS.gene027536.t1 |
| 46418 | CDS | 1369 | 1673 | 1369 | ID=cds.MS.gene027536.t1;Parent=MS.gene027536.t1 |
| 46418 | exon | 889 | 1038 | 889 | ID=MS.gene027536.t1.exon2;Parent=MS.gene027536.t1 |
| 46418 | CDS | 889 | 1038 | 889 | ID=cds.MS.gene027536.t1;Parent=MS.gene027536.t1 |
| 46418 | exon | 320 | 854 | 320 | ID=MS.gene027536.t1.exon3;Parent=MS.gene027536.t1 |
| 46418 | CDS | 320 | 854 | 320 | ID=cds.MS.gene027536.t1;Parent=MS.gene027536.t1 |
| Gene Sequence |
| Protein sequence |
