Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027537.t1 | XP_013453735.1 | 82.6 | 379 | 41 | 1 | 1 | 354 | 1 | 379 | 2.00E-177 | 631.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027537.t1 | A0A072UEA2 | 82.6 | 379 | 41 | 1 | 1 | 354 | 1 | 379 | 1.5e-177 | 631.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene027537 | MS.gene053932 | PPI |
| MS.gene027535 | MS.gene027537 | PPI |
| MS.gene45115 | MS.gene027537 | PPI |
| MS.gene027537 | MS.gene78745 | PPI |
| MS.gene68296 | MS.gene027537 | PPI |
| MS.gene027537 | MS.gene027538 | PPI |
| MS.gene027537 | MS.gene027536 | PPI |
| MS.gene027537 | MS.gene45120 | PPI |
| MS.gene027537 | MS.gene45118 | PPI |
| MS.gene027537 | MS.gene027540 | PPI |
| MS.gene68297 | MS.gene027537 | PPI |
| MS.gene027537 | MS.gene042700 | PPI |
| MS.gene027537 | MS.gene45114 | PPI |
| MS.gene68295 | MS.gene027537 | PPI |
| MS.gene027537 | MS.gene027535 | PPI |
| MS.gene027537 | MS.gene027539 | PPI |
| MS.gene43935 | MS.gene027537 | PPI |
| MS.gene027537 | MS.gene45116 | PPI |
| MS.gene45117 | MS.gene027537 | PPI |
| MS.gene027544 | MS.gene027537 | PPI |
| MS.gene027534 | MS.gene027537 | PPI |
| MS.gene027537 | MS.gene027534 | PPI |
| MS.gene027537 | MS.gene68292 | PPI |
| MS.gene027537 | MS.gene68298 | PPI |
| MS.gene027537 | MS.gene027543 | PPI |
| MS.gene045965 | MS.gene027537 | PPI |
| MS.gene027537 | MS.gene43936 | PPI |
| MS.gene027537 | MS.gene45119 | PPI |
| MS.gene68293 | MS.gene027537 | PPI |
| MS.gene027537 | MS.gene43935 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027537.t1 | MTR_5g035665 | 82.586 | 379 | 41 | 1 | 1 | 354 | 1 | 379 | 0.0 | 639 |
| MS.gene027537.t1 | MTR_5g035690 | 81.088 | 386 | 41 | 2 | 1 | 354 | 1 | 386 | 0.0 | 631 |
| MS.gene027537.t1 | MTR_5g035750 | 80.362 | 387 | 43 | 2 | 1 | 354 | 1 | 387 | 0.0 | 627 |
| MS.gene027537.t1 | MTR_5g035670 | 76.640 | 381 | 62 | 2 | 1 | 354 | 1 | 381 | 0.0 | 585 |
| MS.gene027537.t1 | MTR_5g035760 | 71.579 | 380 | 82 | 3 | 1 | 354 | 1 | 380 | 0.0 | 532 |
| MS.gene027537.t1 | MTR_6g472110 | 47.891 | 403 | 157 | 14 | 1 | 354 | 1 | 399 | 1.46e-107 | 320 |
| MS.gene027537.t1 | MTR_6g016660 | 39.702 | 403 | 189 | 11 | 1 | 354 | 1 | 398 | 1.34e-85 | 264 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027537.t1 | AT1G13520 | 41.457 | 398 | 178 | 12 | 1 | 354 | 1 | 387 | 5.23e-85 | 262 |
| MS.gene027537.t1 | AT1G13510 | 40.306 | 392 | 184 | 12 | 1 | 354 | 1 | 380 | 1.87e-84 | 261 |
| MS.gene027537.t1 | AT1G13480 | 40.302 | 397 | 184 | 12 | 1 | 354 | 1 | 387 | 9.77e-84 | 259 |
| MS.gene027537.t1 | AT1G13490 | 40.306 | 392 | 186 | 11 | 1 | 354 | 1 | 382 | 4.04e-80 | 249 |
| MS.gene027537.t1 | AT1G13540 | 41.221 | 393 | 180 | 13 | 1 | 354 | 1 | 381 | 5.54e-80 | 249 |
| MS.gene027537.t1 | AT1G13530 | 38.325 | 394 | 194 | 11 | 1 | 354 | 1 | 385 | 1.44e-79 | 248 |
| MS.gene027537.t1 | AT1G13490 | 40.214 | 373 | 194 | 10 | 1 | 354 | 1 | 363 | 2.56e-76 | 239 |
| MS.gene027537.t1 | AT1G13550 | 38.131 | 396 | 189 | 13 | 1 | 351 | 1 | 385 | 2.35e-73 | 233 |
| MS.gene027537.t1 | AT1G13470 | 39.796 | 392 | 184 | 13 | 1 | 354 | 1 | 378 | 8.64e-71 | 226 |
| MS.gene027537.t1 | AT1G61600 | 36.407 | 423 | 198 | 11 | 1 | 354 | 1 | 421 | 3.57e-69 | 223 |
| MS.gene027537.t1 | AT1G13500 | 38.228 | 395 | 194 | 12 | 1 | 354 | 1 | 386 | 3.50e-67 | 216 |
Find 0 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTGGAAAGTTGAAATT+TGG | + | 46418:9395-9414 | MS.gene027537:intergenic | 20.0% |
| !! | ATAGATTCATAAAGTAATAG+TGG | - | 46418:9053-9072 | MS.gene027537:CDS | 20.0% |
| !! | GAAATAATCAAAAAAGGTTA+TGG | - | 46418:9227-9246 | MS.gene027537:CDS | 20.0% |
| !! | TGGTAATTAATTAACTTTCA+TGG | - | 46418:9013-9032 | MS.gene027537:CDS | 20.0% |
| !!! | AGACGTAATTATATTTTTGT+TGG | - | 46418:8993-9012 | MS.gene027537:CDS | 20.0% |
| !!! | ATTTTTTCAAAGGAAAATAG+TGG | - | 46418:9551-9570 | MS.gene027537:intron | 20.0% |
| ! | ATCACAAGATAACTTGAATT+TGG | + | 46418:8679-8698 | MS.gene027537:intergenic | 25.0% |
| ! | CAGGTAGAAATAATCAAAAA+AGG | - | 46418:9221-9240 | MS.gene027537:CDS | 25.0% |
| ! | TCAGAACAATCATTGAAAAT+TGG | + | 46418:9410-9429 | MS.gene027537:intergenic | 25.0% |
| !! | TTCAAGTTATCTTGTGATAT+TGG | - | 46418:8681-8700 | MS.gene027537:CDS | 25.0% |
| ACAAAAGTTGAAGAGTTTGA+TGG | - | 46418:9794-9813 | MS.gene027537:CDS | 30.0% | |
| ACATCAAATTAGGAGCAAAT+GGG | - | 46418:9919-9938 | MS.gene027537:CDS | 30.0% | |
| AGTTTGATGGAACAAATAAG+TGG | - | 46418:9807-9826 | MS.gene027537:CDS | 30.0% | |
| ATGGTTAAATTCTTGTTCTG+AGG | + | 46418:8775-8794 | MS.gene027537:intergenic | 30.0% | |
| GAAAAAAAGGTGGAAACTTA+AGG | - | 46418:9301-9320 | MS.gene027537:CDS | 30.0% | |
| GAAGGAGAGAATGAATTTAA+AGG | - | 46418:9472-9491 | MS.gene027537:intron | 30.0% | |
| TACTACGTTATAAGAAGGAA+GGG | - | 46418:8877-8896 | MS.gene027537:CDS | 30.0% | |
| TATTCAAACAGAAGTTGCTA+AGG | - | 46418:9601-9620 | MS.gene027537:intron | 30.0% | |
| TGAGAGAAAAACTTGTACAA+TGG | + | 46418:8948-8967 | MS.gene027537:intergenic | 30.0% | |
| TGGTTAAATTCTTGTTCTGA+GGG | + | 46418:8774-8793 | MS.gene027537:intergenic | 30.0% | |
| TTAACCATCAAGTATTCATC+AGG | - | 46418:8787-8806 | MS.gene027537:CDS | 30.0% | |
| TTATCTTGTGATATTGGATG+AGG | - | 46418:8687-8706 | MS.gene027537:CDS | 30.0% | |
| TTGTCCATTTATGTTTGTGA+AGG | - | 46418:9454-9473 | MS.gene027537:intron | 30.0% | |
| TTTCTATTGAAGAGAATGGA+TGG | - | 46418:9863-9882 | MS.gene027537:CDS | 30.0% | |
| ! | AAGTTTTTCTCTCATGTCAA+CGG | - | 46418:8954-8973 | MS.gene027537:CDS | 30.0% |
| ! | ACTAGGATTCTTTTTGTACA+TGG | + | 46418:8629-8648 | MS.gene027537:intergenic | 30.0% |
| ! | GAGTTTTCTATTGAAGAGAA+TGG | - | 46418:9859-9878 | MS.gene027537:CDS | 30.0% |
| ! | GATGAAAATAGATTGGTTGA+TGG | - | 46418:9647-9666 | MS.gene027537:intron | 30.0% |
| ! | TGTTTGGGATGAAAATAGAT+TGG | - | 46418:9640-9659 | MS.gene027537:intron | 30.0% |
| !! | ACCTGTTGATAATCATTGAA+GGG | + | 46418:9206-9225 | MS.gene027537:intergenic | 30.0% |
| !! | AGTGTTGATGAGAAAAATGT+TGG | - | 46418:9686-9705 | MS.gene027537:intron | 30.0% |
| !! | TACCTGTTGATAATCATTGA+AGG | + | 46418:9207-9226 | MS.gene027537:intergenic | 30.0% |
| !!! | ATGGGAGAAGATTTTTTCAA+AGG | - | 46418:9541-9560 | MS.gene027537:intron | 30.0% |
| AAATGGGAACAAAGAAGGTT+TGG | - | 46418:9737-9756 | MS.gene027537:CDS | 35.0% | |
| AAGTTGTTGAGGGAATGAAA+TGG | - | 46418:9720-9739 | MS.gene027537:CDS | 35.0% | |
| AATCATTTGAGCCTAAAGCT+TGG | + | 46418:9355-9374 | MS.gene027537:intergenic | 35.0% | |
| ACTACGTTATAAGAAGGAAG+GGG | - | 46418:8878-8897 | MS.gene027537:CDS | 35.0% | |
| AGTTGTTGAGGGAATGAAAT+GGG | - | 46418:9721-9740 | MS.gene027537:CDS | 35.0% | |
| ATCGCTACTACGTTATAAGA+AGG | - | 46418:8872-8891 | MS.gene027537:CDS | 35.0% | |
| CACATCAAATTAGGAGCAAA+TGG | - | 46418:9918-9937 | MS.gene027537:CDS | 35.0% | |
| CTACTACGTTATAAGAAGGA+AGG | - | 46418:8876-8895 | MS.gene027537:CDS | 35.0% | |
| CTCTCCTTCACAAACATAAA+TGG | + | 46418:9461-9480 | MS.gene027537:intergenic | 35.0% | |
| CTGGGTTATTGAGGAAAAAA+AGG | - | 46418:9288-9307 | MS.gene027537:CDS | 35.0% | |
| GAAAAATGTTGGACTGAGTT+TGG | - | 46418:9697-9716 | MS.gene027537:intron | 35.0% | |
| GAATGAAATGGGAACAAAGA+AGG | - | 46418:9732-9751 | MS.gene027537:CDS | 35.0% | |
| GAATGAACATAGCTTCTTCA+TGG | + | 46418:8825-8844 | MS.gene027537:intergenic | 35.0% | |
| GGTTATTGAGGAAAAAAAGG+TGG | - | 46418:9291-9310 | MS.gene027537:CDS | 35.0% | |
| TAGTACAAGTTCAAAGGAAG+AGG | - | 46418:9121-9140 | MS.gene027537:CDS | 35.0% | |
| TCAGACACTCACATCAAATT+AGG | - | 46418:9909-9928 | MS.gene027537:CDS | 35.0% | |
| TTCACCTGATGAATACTTGA+TGG | + | 46418:8794-8813 | MS.gene027537:intergenic | 35.0% | |
| ! | AAAACTACAGCAAAAGCATG+TGG | + | 46418:9153-9172 | MS.gene027537:intergenic | 35.0% |
| ! | CAATGATTGTTCTGAATCAG+TGG | - | 46418:9415-9434 | MS.gene027537:CDS | 35.0% |
| ! | GACAATGGTTGATTCAATAC+TGG | + | 46418:8847-8866 | MS.gene027537:intergenic | 35.0% |
| ! | TCAAAGGAAAATAGTGGAGA+AGG | - | 46418:9557-9576 | MS.gene027537:intron | 35.0% |
| !! | AAATTCTTGTTCTGAGGGAT+AGG | + | 46418:8769-8788 | MS.gene027537:intergenic | 35.0% |
| !! | GCTCAAACTTACTCTTGTTT+TGG | - | 46418:8712-8731 | MS.gene027537:CDS | 35.0% |
| !!! | CTTTTTTTCCTCAATAACCC+AGG | + | 46418:9290-9309 | MS.gene027537:intergenic | 35.0% |
| AAAAGGTGGAAACTTAAGGC+TGG | - | 46418:9305-9324 | MS.gene027537:CDS | 40.0% | |
| AATCAGTGGTTGTTGGAAAG+TGG | - | 46418:9429-9448 | MS.gene027537:intron | 40.0% | |
| ACAGAAGTTGCTAAGGTTGA+AGG | - | 46418:9608-9627 | MS.gene027537:intron | 40.0% | |
| ACTGAGTTTGGAAGTTGTTG+AGG | - | 46418:9709-9728 | MS.gene027537:intron | 40.0% | |
| AGAAGGAAGGGGAAATATCA+AGG | - | 46418:8889-8908 | MS.gene027537:CDS | 40.0% | |
| AGCCTAAAGCTTGGCTTAAA+TGG | + | 46418:9346-9365 | MS.gene027537:intergenic | 40.0% | |
| AGCTAACACTTGAGCAAAGA+TGG | - | 46418:9522-9541 | MS.gene027537:intron | 40.0% | |
| AGTTGCTAAGGTTGAAGGAA+AGG | - | 46418:9613-9632 | MS.gene027537:intron | 40.0% | |
| CACCATTTAAGCCAAGCTTT+AGG | - | 46418:9341-9360 | MS.gene027537:CDS | 40.0% | |
| CCAAGCTAGTACAAGTTCAA+AGG | - | 46418:9115-9134 | MS.gene027537:CDS | 40.0% | |
| CCTTTGAACTTGTACTAGCT+TGG | + | 46418:9118-9137 | MS.gene027537:intergenic | 40.0% | |
| CTAAAGCTTGGCTTAAATGG+TGG | + | 46418:9343-9362 | MS.gene027537:intergenic | 40.0% | |
| CTGAGTTTGGAAGTTGTTGA+GGG | - | 46418:9710-9729 | MS.gene027537:intron | 40.0% | |
| GAAGGAAGGGGAAATATCAA+GGG | - | 46418:8890-8909 | MS.gene027537:CDS | 40.0% | |
| GCCCTTCAATGATTATCAAC+AGG | - | 46418:9202-9221 | MS.gene027537:CDS | 40.0% | |
| GCTAACACTTGAGCAAAGAT+GGG | - | 46418:9523-9542 | MS.gene027537:intron | 40.0% | |
| TAACTTGAATTTGGCCCTGA+TGG | + | 46418:8670-8689 | MS.gene027537:intergenic | 40.0% | |
| TAGTAGCGATTCGAAGACAA+TGG | + | 46418:8862-8881 | MS.gene027537:intergenic | 40.0% | |
| TGAACATAGCTTCTTCATGG+CGG | + | 46418:8822-8841 | MS.gene027537:intergenic | 40.0% | |
| ! | AAGGAAAATAGTGGAGAAGG+TGG | - | 46418:9560-9579 | MS.gene027537:intron | 40.0% |
| ! | TGTTCTGAATCAGTGGTTGT+TGG | - | 46418:9422-9441 | MS.gene027537:intron | 40.0% |
| ! | TTTTTCCTCAATAACCCAGG+AGG | + | 46418:9287-9306 | MS.gene027537:intergenic | 40.0% |
| !! | TTGAAGGGCTCTAAAGCTAT+TGG | + | 46418:9191-9210 | MS.gene027537:intergenic | 40.0% |
| !!! | CTCTTGTTTTGGTTTGTGCA+AGG | - | 46418:8723-8742 | MS.gene027537:CDS | 40.0% |
| !!! | GATTGGTTGATGGAGTTTTG+TGG | - | 46418:9657-9676 | MS.gene027537:intron | 40.0% |
| AGAAGGTTTGGATGGAATGC+TGG | - | 46418:9749-9768 | MS.gene027537:CDS | 45.0% | |
| CTTGGCTTAAATGGTGGTTG+CGG | + | 46418:9337-9356 | MS.gene027537:intergenic | 45.0% | |
| GAACATAGCTTCTTCATGGC+GGG | + | 46418:8821-8840 | MS.gene027537:intergenic | 45.0% | |
| GAAGGTTTGGATGGAATGCT+GGG | - | 46418:9750-9769 | MS.gene027537:CDS | 45.0% | |
| GAATTCCTCCTGGGTTATTG+AGG | - | 46418:9279-9298 | MS.gene027537:CDS | 45.0% | |
| GCAAAATCAGTTGCCTCAGA+TGG | - | 46418:9257-9276 | MS.gene027537:CDS | 45.0% | |
| GGGAACAAAGAAGGTTTGGA+TGG | - | 46418:9741-9760 | MS.gene027537:CDS | 45.0% | |
| TGAAGGAAAGGACGCTGTTT+GGG | - | 46418:9625-9644 | MS.gene027537:intron | 45.0% | |
| TTGAAGGAAAGGACGCTGTT+TGG | - | 46418:9624-9643 | MS.gene027537:intron | 45.0% | |
| TTGGCTTAAATGGTGGTTGC+GGG | + | 46418:9336-9355 | MS.gene027537:intergenic | 45.0% | |
| TTTGGATGGAATGCTGGGAA+TGG | - | 46418:9755-9774 | MS.gene027537:CDS | 45.0% | |
| ! | ATTTGGTAGCTTCGAACGCA+AGG | + | 46418:9378-9397 | MS.gene027537:intergenic | 45.0% |
| CCTCAGATGGAATTCCTCCT+GGG | - | 46418:9270-9289 | MS.gene027537:CDS | 50.0% | |
| CTTGAATTTGGCCCTGATGG+TGG | + | 46418:8667-8686 | MS.gene027537:intergenic | 50.0% | |
| TATCAGACCCACCACCATCA+GGG | - | 46418:8653-8672 | MS.gene027537:CDS | 50.0% | |
| TGGCTTAAATGGTGGTTGCG+GGG | + | 46418:9335-9354 | MS.gene027537:intergenic | 50.0% | |
| !! | AATTTGGCCCTGATGGTGGT+GGG | + | 46418:8663-8682 | MS.gene027537:intergenic | 50.0% |
| CCCAGGAGGAATTCCATCTG+AGG | + | 46418:9273-9292 | MS.gene027537:intergenic | 55.0% | |
| CTATCAGACCCACCACCATC+AGG | - | 46418:8652-8671 | MS.gene027537:CDS | 55.0% | |
| GCCTCAGATGGAATTCCTCC+TGG | - | 46418:9269-9288 | MS.gene027537:CDS | 55.0% | |
| ! | CATAGCTTCTTCATGGCGGG+TGG | + | 46418:8818-8837 | MS.gene027537:intergenic | 55.0% |
| ! | GGTGGGTCTGATAGAGCACT+AGG | + | 46418:8646-8665 | MS.gene027537:intergenic | 55.0% |
| !! | GAATTTGGCCCTGATGGTGG+TGG | + | 46418:8664-8683 | MS.gene027537:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 46418 | gene | 8604 | 9946 | 8604 | ID=MS.gene027537 |
| 46418 | mRNA | 8604 | 9946 | 8604 | ID=MS.gene027537.t1;Parent=MS.gene027537 |
| 46418 | exon | 9714 | 9946 | 9714 | ID=MS.gene027537.t1.exon1;Parent=MS.gene027537.t1 |
| 46418 | CDS | 9714 | 9946 | 9714 | ID=cds.MS.gene027537.t1;Parent=MS.gene027537.t1 |
| 46418 | exon | 8604 | 9435 | 8604 | ID=MS.gene027537.t1.exon2;Parent=MS.gene027537.t1 |
| 46418 | CDS | 8604 | 9435 | 8604 | ID=cds.MS.gene027537.t1;Parent=MS.gene027537.t1 |
| Gene Sequence |
| Protein sequence |
