Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027540.t1 | XP_003613366.2 | 89.4 | 265 | 28 | 0 | 2 | 266 | 168 | 432 | 1.00E-136 | 496.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027540.t1 | G7K479 | 89.4 | 265 | 28 | 0 | 2 | 266 | 123 | 387 | 7.2e-137 | 496.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene68297 | MS.gene027540 | PPI |
| MS.gene027540 | MS.gene027539 | PPI |
| MS.gene027540 | MS.gene042700 | PPI |
| MS.gene45117 | MS.gene027540 | PPI |
| MS.gene027540 | MS.gene45114 | PPI |
| MS.gene027540 | MS.gene68298 | PPI |
| MS.gene027544 | MS.gene027540 | PPI |
| MS.gene027534 | MS.gene027540 | PPI |
| MS.gene027537 | MS.gene027540 | PPI |
| MS.gene027540 | MS.gene43936 | PPI |
| MS.gene45116 | MS.gene027540 | PPI |
| MS.gene68295 | MS.gene027540 | PPI |
| MS.gene027540 | MS.gene45119 | PPI |
| MS.gene027540 | MS.gene68292 | PPI |
| MS.gene045965 | MS.gene027540 | PPI |
| MS.gene43935 | MS.gene027540 | PPI |
| MS.gene45114 | MS.gene027540 | PPI |
| MS.gene027540 | MS.gene027543 | PPI |
| MS.gene027540 | MS.gene053932 | PPI |
| MS.gene68293 | MS.gene027540 | PPI |
| MS.gene027539 | MS.gene027540 | PPI |
| MS.gene027535 | MS.gene027540 | PPI |
| MS.gene027540 | MS.gene45120 | PPI |
| MS.gene45115 | MS.gene027540 | PPI |
| MS.gene027540 | MS.gene45118 | PPI |
| MS.gene68296 | MS.gene027540 | PPI |
| MS.gene027540 | MS.gene78745 | PPI |
| MS.gene68292 | MS.gene027540 | PPI |
| MS.gene027543 | MS.gene027540 | PPI |
| MS.gene027540 | MS.gene027538 | PPI |
| MS.gene027540 | MS.gene027536 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027540.t1 | MTR_5g035750 | 89.434 | 265 | 28 | 0 | 2 | 266 | 123 | 387 | 1.89e-178 | 496 |
| MS.gene027540.t1 | MTR_5g035690 | 88.722 | 266 | 30 | 0 | 1 | 266 | 121 | 386 | 3.74e-177 | 493 |
| MS.gene027540.t1 | MTR_5g035665 | 88.302 | 265 | 31 | 0 | 2 | 266 | 115 | 379 | 9.53e-177 | 492 |
| MS.gene027540.t1 | MTR_5g035670 | 80.899 | 267 | 49 | 1 | 2 | 266 | 115 | 381 | 8.66e-158 | 444 |
| MS.gene027540.t1 | MTR_5g035760 | 78.358 | 268 | 56 | 2 | 1 | 266 | 113 | 380 | 8.72e-151 | 426 |
| MS.gene027540.t1 | MTR_6g472110 | 51.773 | 282 | 113 | 11 | 5 | 266 | 121 | 399 | 9.60e-81 | 248 |
| MS.gene027540.t1 | MTR_6g016660 | 42.908 | 282 | 137 | 8 | 6 | 266 | 120 | 398 | 2.87e-64 | 206 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027540.t1 | AT1G13520 | 44.404 | 277 | 129 | 10 | 4 | 266 | 122 | 387 | 1.19e-61 | 199 |
| MS.gene027540.t1 | AT1G13510 | 42.701 | 274 | 137 | 8 | 1 | 266 | 119 | 380 | 6.22e-61 | 197 |
| MS.gene027540.t1 | AT1G13480 | 42.294 | 279 | 138 | 9 | 1 | 266 | 119 | 387 | 1.55e-59 | 194 |
| MS.gene027540.t1 | AT1G13540 | 44.526 | 274 | 132 | 8 | 1 | 266 | 120 | 381 | 6.94e-59 | 192 |
| MS.gene027540.t1 | AT1G13490 | 43.321 | 277 | 135 | 9 | 2 | 266 | 116 | 382 | 5.69e-58 | 189 |
| MS.gene027540.t1 | AT1G13530 | 39.855 | 276 | 147 | 9 | 1 | 266 | 119 | 385 | 4.54e-57 | 187 |
| MS.gene027540.t1 | AT1G13490 | 43.333 | 270 | 131 | 9 | 9 | 266 | 104 | 363 | 6.25e-55 | 181 |
| MS.gene027540.t1 | AT1G13470 | 43.431 | 274 | 137 | 9 | 1 | 266 | 115 | 378 | 2.14e-54 | 180 |
| MS.gene027540.t1 | AT1G13500 | 41.727 | 278 | 140 | 9 | 2 | 266 | 118 | 386 | 2.85e-53 | 177 |
| MS.gene027540.t1 | AT1G61600 | 38.079 | 302 | 144 | 8 | 6 | 266 | 122 | 421 | 3.29e-51 | 173 |
| MS.gene027540.t1 | AT1G13550 | 37.906 | 277 | 148 | 9 | 1 | 263 | 119 | 385 | 3.82e-48 | 164 |
Find 52 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGAACAATCATTGAAAAT+TGG | 0.211604 | 5.4:-40668943 | None:intergenic |
| AAAATTGGAAAGTTGAAATT+TGG | 0.232410 | 5.4:-40668928 | None:intergenic |
| AGCCTAAAGCTTGGCTTAAA+TGG | 0.235862 | 5.4:-40668879 | None:intergenic |
| CTCTCCTTCACAAACATAAA+TGG | 0.257853 | 5.4:-40668994 | None:intergenic |
| GAAGGAGAGAATGAATTTAA+AGG | 0.260016 | 5.4:+40669008 | MS.gene027540:CDS |
| TCAGACACTCACATCAAATT+AGG | 0.281648 | 5.4:+40669445 | MS.gene027540:CDS |
| TTGAAGGGCTCTAAAGCTAT+TGG | 0.344879 | 5.4:-40668724 | None:intergenic |
| GCCTCAGATGGAATTCCTCC+TGG | 0.354070 | 5.4:+40668805 | MS.gene027540:CDS |
| CACATCAAATTAGGAGCAAA+TGG | 0.354134 | 5.4:+40669454 | MS.gene027540:CDS |
| TTGGCTTAAATGGTGGTTGC+GGG | 0.360622 | 5.4:-40668869 | None:intergenic |
| TGAAGGAAAGGACGCTGTTT+GGG | 0.368363 | 5.4:+40669161 | MS.gene027540:CDS |
| TTGAAGGAAAGGACGCTGTT+TGG | 0.369269 | 5.4:+40669160 | MS.gene027540:CDS |
| ACAAAAGTTGAAGAGTTTGA+TGG | 0.392275 | 5.4:+40669330 | MS.gene027540:CDS |
| AAATGGGAACAAAGAAGGTT+TGG | 0.394970 | 5.4:+40669273 | MS.gene027540:CDS |
| AGAAGGTTTGGATGGAATGC+TGG | 0.431581 | 5.4:+40669285 | MS.gene027540:CDS |
| AGCTAACACTTGAGCAAAGA+TGG | 0.431951 | 5.4:+40669058 | MS.gene027540:CDS |
| CACCATTTAAGCCAAGCTTT+AGG | 0.434225 | 5.4:+40668877 | MS.gene027540:CDS |
| GCCCTTCAATGATTATCAAC+AGG | 0.448229 | 5.4:+40668738 | MS.gene027540:CDS |
| ACAGAAGTTGCTAAGGTTGA+AGG | 0.453239 | 5.4:+40669144 | MS.gene027540:CDS |
| GATGAAAATAGATTGGTTGA+TGG | 0.465731 | 5.4:+40669183 | MS.gene027540:CDS |
| TACCTGTTGATAATCATTGA+AGG | 0.466990 | 5.4:-40668740 | None:intergenic |
| TTTGGATGGAATGCTGGGAA+TGG | 0.475064 | 5.4:+40669291 | MS.gene027540:CDS |
| GAATTCCTCCTGGGTTATTG+AGG | 0.475186 | 5.4:+40668815 | MS.gene027540:CDS |
| TCAAAGGAAAATAGTGGAGA+AGG | 0.489041 | 5.4:+40669093 | MS.gene027540:CDS |
| TGTTTGGGATGAAAATAGAT+TGG | 0.492208 | 5.4:+40669176 | MS.gene027540:CDS |
| CTTGGCTTAAATGGTGGTTG+CGG | 0.507449 | 5.4:-40668870 | None:intergenic |
| GCAAAATCAGTTGCCTCAGA+TGG | 0.507539 | 5.4:+40668793 | MS.gene027540:CDS |
| GGGAACAAAGAAGGTTTGGA+TGG | 0.521569 | 5.4:+40669277 | MS.gene027540:CDS |
| CCTCAGATGGAATTCCTCCT+GGG | 0.522046 | 5.4:+40668806 | MS.gene027540:CDS |
| TGTTCTGAATCAGTGGTTGT+TGG | 0.524665 | 5.4:+40668958 | MS.gene027540:CDS |
| ACATCAAATTAGGAGCAAAT+GGG | 0.536534 | 5.4:+40669455 | MS.gene027540:CDS |
| GAATGAAATGGGAACAAAGA+AGG | 0.541076 | 5.4:+40669268 | MS.gene027540:CDS |
| ATTTGGTAGCTTCGAACGCA+AGG | 0.544069 | 5.4:-40668911 | None:intergenic |
| TATTCAAACAGAAGTTGCTA+AGG | 0.545070 | 5.4:+40669137 | MS.gene027540:CDS |
| ACCTGTTGATAATCATTGAA+GGG | 0.546054 | 5.4:-40668739 | None:intergenic |
| AGTTGTTGAGGGAATGAAAT+GGG | 0.546947 | 5.4:+40669257 | MS.gene027540:CDS |
| CAATGATTGTTCTGAATCAG+TGG | 0.548989 | 5.4:+40668951 | MS.gene027540:CDS |
| TTGTCCATTTATGTTTGTGA+AGG | 0.558931 | 5.4:+40668990 | MS.gene027540:CDS |
| TTGAGTTTGGAAGTTGTTGA+GGG | 0.559577 | 5.4:+40669246 | MS.gene027540:CDS |
| GAAGGTTTGGATGGAATGCT+GGG | 0.561735 | 5.4:+40669286 | MS.gene027540:CDS |
| AATCATTTGAGCCTAAAGCT+TGG | 0.566503 | 5.4:-40668888 | None:intergenic |
| AGTTTGATGGAACAAATAAG+TGG | 0.584146 | 5.4:+40669343 | MS.gene027540:CDS |
| CTAAAGCTTGGCTTAAATGG+TGG | 0.584507 | 5.4:-40668876 | None:intergenic |
| ATTGAGTTTGGAAGTTGTTG+AGG | 0.595211 | 5.4:+40669245 | MS.gene027540:CDS |
| AGTTGCTAAGGTTGAAGGAA+AGG | 0.599670 | 5.4:+40669149 | MS.gene027540:CDS |
| AAGGAAAATAGTGGAGAAGG+TGG | 0.602522 | 5.4:+40669096 | MS.gene027540:CDS |
| TGGCTTAAATGGTGGTTGCG+GGG | 0.603595 | 5.4:-40668868 | None:intergenic |
| CCCAGGAGGAATTCCATCTG+AGG | 0.605151 | 5.4:-40668806 | None:intergenic |
| AAGTTGTTGAGGGAATGAAA+TGG | 0.611433 | 5.4:+40669256 | MS.gene027540:CDS |
| GCTAACACTTGAGCAAAGAT+GGG | 0.612705 | 5.4:+40669059 | MS.gene027540:CDS |
| AATCAGTGGTTGTTGGAAAG+TGG | 0.623216 | 5.4:+40668965 | MS.gene027540:CDS |
| AAAACTACAGCAAAAGCATG+TGG | 0.702446 | 5.4:-40668686 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTGGAAAGTTGAAATT+TGG | - | chr5.4:40668931-40668950 | None:intergenic | 20.0% |
| !! | GAAATAATCAAAAAAGGTTA+TGG | + | chr5.4:40668763-40668782 | MS.gene027540:CDS | 20.0% |
| !!! | ATTTTTTCAAAGGAAAATAG+TGG | + | chr5.4:40669087-40669106 | MS.gene027540:CDS | 20.0% |
| ! | CAGGTAGAAATAATCAAAAA+AGG | + | chr5.4:40668757-40668776 | MS.gene027540:CDS | 25.0% |
| ! | TCAGAACAATCATTGAAAAT+TGG | - | chr5.4:40668946-40668965 | None:intergenic | 25.0% |
| !! | TTTTTATTGAAGAGAATGGA+TGG | + | chr5.4:40669399-40669418 | MS.gene027540:CDS | 25.0% |
| !!! | GAGTTTTTTATTGAAGAGAA+TGG | + | chr5.4:40669395-40669414 | MS.gene027540:CDS | 25.0% |
| ACAAAAGTTGAAGAGTTTGA+TGG | + | chr5.4:40669330-40669349 | MS.gene027540:CDS | 30.0% | |
| ACATCAAATTAGGAGCAAAT+GGG | + | chr5.4:40669455-40669474 | MS.gene027540:CDS | 30.0% | |
| AGTTTGATGGAACAAATAAG+TGG | + | chr5.4:40669343-40669362 | MS.gene027540:CDS | 30.0% | |
| GAAAAAAAGGTGGAAACTTA+AGG | + | chr5.4:40668837-40668856 | MS.gene027540:CDS | 30.0% | |
| GAAAAATGTTGGATTGAGTT+TGG | + | chr5.4:40669233-40669252 | MS.gene027540:CDS | 30.0% | |
| GAAGGAGAGAATGAATTTAA+AGG | + | chr5.4:40669008-40669027 | MS.gene027540:CDS | 30.0% | |
| TATTCAAACAGAAGTTGCTA+AGG | + | chr5.4:40669137-40669156 | MS.gene027540:CDS | 30.0% | |
| TTGTCCATTTATGTTTGTGA+AGG | + | chr5.4:40668990-40669009 | MS.gene027540:CDS | 30.0% | |
| ! | GATGAAAATAGATTGGTTGA+TGG | + | chr5.4:40669183-40669202 | MS.gene027540:CDS | 30.0% |
| ! | TGTTTGGGATGAAAATAGAT+TGG | + | chr5.4:40669176-40669195 | MS.gene027540:CDS | 30.0% |
| !! | ACCTGTTGATAATCATTGAA+GGG | - | chr5.4:40668742-40668761 | None:intergenic | 30.0% |
| !! | AGTGTTGATGAGAAAAATGT+TGG | + | chr5.4:40669222-40669241 | MS.gene027540:CDS | 30.0% |
| !! | TACCTGTTGATAATCATTGA+AGG | - | chr5.4:40668743-40668762 | None:intergenic | 30.0% |
| !!! | ATGGGAGAAGATTTTTTCAA+AGG | + | chr5.4:40669077-40669096 | MS.gene027540:CDS | 30.0% |
| AAATGGGAACAAAGAAGGTT+TGG | + | chr5.4:40669273-40669292 | MS.gene027540:CDS | 35.0% | |
| AAGTTGTTGAGGGAATGAAA+TGG | + | chr5.4:40669256-40669275 | MS.gene027540:CDS | 35.0% | |
| AATCATTTGAGCCTAAAGCT+TGG | - | chr5.4:40668891-40668910 | None:intergenic | 35.0% | |
| AGTTGTTGAGGGAATGAAAT+GGG | + | chr5.4:40669257-40669276 | MS.gene027540:CDS | 35.0% | |
| ATTGAGTTTGGAAGTTGTTG+AGG | + | chr5.4:40669245-40669264 | MS.gene027540:CDS | 35.0% | |
| CACATCAAATTAGGAGCAAA+TGG | + | chr5.4:40669454-40669473 | MS.gene027540:CDS | 35.0% | |
| CTCTCCTTCACAAACATAAA+TGG | - | chr5.4:40668997-40669016 | None:intergenic | 35.0% | |
| CTGGGTTATTGAGGAAAAAA+AGG | + | chr5.4:40668824-40668843 | MS.gene027540:CDS | 35.0% | |
| GAATGAAATGGGAACAAAGA+AGG | + | chr5.4:40669268-40669287 | MS.gene027540:CDS | 35.0% | |
| GGTTATTGAGGAAAAAAAGG+TGG | + | chr5.4:40668827-40668846 | MS.gene027540:CDS | 35.0% | |
| TCAGACACTCACATCAAATT+AGG | + | chr5.4:40669445-40669464 | MS.gene027540:CDS | 35.0% | |
| TTGAGTTTGGAAGTTGTTGA+GGG | + | chr5.4:40669246-40669265 | MS.gene027540:CDS | 35.0% | |
| ! | AAAACTACAGCAAAAGCATG+TGG | - | chr5.4:40668689-40668708 | None:intergenic | 35.0% |
| ! | CAATGATTGTTCTGAATCAG+TGG | + | chr5.4:40668951-40668970 | MS.gene027540:CDS | 35.0% |
| ! | TCAAAGGAAAATAGTGGAGA+AGG | + | chr5.4:40669093-40669112 | MS.gene027540:CDS | 35.0% |
| !!! | CTTTTTTTCCTCAATAACCC+AGG | - | chr5.4:40668826-40668845 | None:intergenic | 35.0% |
| AATCAGTGGTTGTTGGAAAG+TGG | + | chr5.4:40668965-40668984 | MS.gene027540:CDS | 40.0% | |
| ACAGAAGTTGCTAAGGTTGA+AGG | + | chr5.4:40669144-40669163 | MS.gene027540:CDS | 40.0% | |
| AGCCTAAAGCTTGGCTTAAA+TGG | - | chr5.4:40668882-40668901 | None:intergenic | 40.0% | |
| AGCTAACACTTGAGCAAAGA+TGG | + | chr5.4:40669058-40669077 | MS.gene027540:CDS | 40.0% | |
| AGTTGCTAAGGTTGAAGGAA+AGG | + | chr5.4:40669149-40669168 | MS.gene027540:CDS | 40.0% | |
| CACCATTTAAGCCAAGCTTT+AGG | + | chr5.4:40668877-40668896 | MS.gene027540:CDS | 40.0% | |
| CTAAAGCTTGGCTTAAATGG+TGG | - | chr5.4:40668879-40668898 | None:intergenic | 40.0% | |
| GCCCTTCAATGATTATCAAC+AGG | + | chr5.4:40668738-40668757 | MS.gene027540:CDS | 40.0% | |
| GCTAACACTTGAGCAAAGAT+GGG | + | chr5.4:40669059-40669078 | MS.gene027540:CDS | 40.0% | |
| ! | AAGGAAAATAGTGGAGAAGG+TGG | + | chr5.4:40669096-40669115 | MS.gene027540:CDS | 40.0% |
| ! | TGTTCTGAATCAGTGGTTGT+TGG | + | chr5.4:40668958-40668977 | MS.gene027540:CDS | 40.0% |
| ! | TTTTTCCTCAATAACCCAGG+AGG | - | chr5.4:40668823-40668842 | None:intergenic | 40.0% |
| !! | TTGAAGGGCTCTAAAGCTAT+TGG | - | chr5.4:40668727-40668746 | None:intergenic | 40.0% |
| !!! | GATTGGTTGATGGAGTTTTG+TGG | + | chr5.4:40669193-40669212 | MS.gene027540:CDS | 40.0% |
| AGAAGGTTTGGATGGAATGC+TGG | + | chr5.4:40669285-40669304 | MS.gene027540:CDS | 45.0% | |
| CTTGGCTTAAATGGTGGTTG+CGG | - | chr5.4:40668873-40668892 | None:intergenic | 45.0% | |
| GAAGGTTTGGATGGAATGCT+GGG | + | chr5.4:40669286-40669305 | MS.gene027540:CDS | 45.0% | |
| GAATTCCTCCTGGGTTATTG+AGG | + | chr5.4:40668815-40668834 | MS.gene027540:CDS | 45.0% | |
| GCAAAATCAGTTGCCTCAGA+TGG | + | chr5.4:40668793-40668812 | MS.gene027540:CDS | 45.0% | |
| GGGAACAAAGAAGGTTTGGA+TGG | + | chr5.4:40669277-40669296 | MS.gene027540:CDS | 45.0% | |
| TGAAGGAAAGGACGCTGTTT+GGG | + | chr5.4:40669161-40669180 | MS.gene027540:CDS | 45.0% | |
| TTGAAGGAAAGGACGCTGTT+TGG | + | chr5.4:40669160-40669179 | MS.gene027540:CDS | 45.0% | |
| TTGGCTTAAATGGTGGTTGC+GGG | - | chr5.4:40668872-40668891 | None:intergenic | 45.0% | |
| TTTGGATGGAATGCTGGGAA+TGG | + | chr5.4:40669291-40669310 | MS.gene027540:CDS | 45.0% | |
| ! | ATTTGGTAGCTTCGAACGCA+AGG | - | chr5.4:40668914-40668933 | None:intergenic | 45.0% |
| CCTCAGATGGAATTCCTCCT+GGG | + | chr5.4:40668806-40668825 | MS.gene027540:CDS | 50.0% | |
| TGGCTTAAATGGTGGTTGCG+GGG | - | chr5.4:40668871-40668890 | None:intergenic | 50.0% | |
| CCCAGGAGGAATTCCATCTG+AGG | - | chr5.4:40668809-40668828 | None:intergenic | 55.0% | |
| GCCTCAGATGGAATTCCTCC+TGG | + | chr5.4:40668805-40668824 | MS.gene027540:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 40668682 | 40669482 | 40668682 | ID=MS.gene027540 |
| chr5.4 | mRNA | 40668682 | 40669482 | 40668682 | ID=MS.gene027540.t1;Parent=MS.gene027540 |
| chr5.4 | exon | 40668682 | 40669482 | 40668682 | ID=MS.gene027540.t1.exon1;Parent=MS.gene027540.t1 |
| chr5.4 | CDS | 40668682 | 40669482 | 40668682 | ID=cds.MS.gene027540.t1;Parent=MS.gene027540.t1 |
| Gene Sequence |
| Protein sequence |
