Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68292.t1 | XP_003613366.2 | 89.5 | 267 | 26 | 1 | 2 | 268 | 168 | 432 | 2.20E-136 | 495 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68292.t1 | G7K479 | 89.5 | 267 | 26 | 1 | 2 | 268 | 123 | 387 | 1.6e-136 | 495.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052040 | MS.gene68292 | 0.800326 | 1.52E-48 | -1.69E-46 |
| MS.gene05624 | MS.gene68292 | 0.809006 | 2.34E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene68292 | 0.812057 | 5.13E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene68292 | MS.gene45114 | PPI |
| MS.gene027539 | MS.gene68292 | PPI |
| MS.gene027535 | MS.gene68292 | PPI |
| MS.gene68292 | MS.gene027539 | PPI |
| MS.gene68296 | MS.gene68292 | PPI |
| MS.gene68292 | MS.gene68298 | PPI |
| MS.gene68292 | MS.gene027543 | PPI |
| MS.gene68297 | MS.gene68292 | PPI |
| MS.gene45115 | MS.gene68292 | PPI |
| MS.gene027540 | MS.gene68292 | PPI |
| MS.gene68292 | MS.gene43936 | PPI |
| MS.gene68292 | MS.gene45119 | PPI |
| MS.gene027543 | MS.gene68292 | PPI |
| MS.gene45117 | MS.gene68292 | PPI |
| MS.gene027534 | MS.gene68292 | PPI |
| MS.gene027537 | MS.gene68292 | PPI |
| MS.gene68292 | MS.gene053932 | PPI |
| MS.gene68295 | MS.gene68292 | PPI |
| MS.gene68292 | MS.gene78745 | PPI |
| MS.gene045965 | MS.gene68292 | PPI |
| MS.gene68292 | MS.gene027538 | PPI |
| MS.gene43935 | MS.gene68292 | PPI |
| MS.gene68292 | MS.gene027536 | PPI |
| MS.gene45114 | MS.gene68292 | PPI |
| MS.gene027544 | MS.gene68292 | PPI |
| MS.gene68292 | MS.gene45120 | PPI |
| MS.gene68292 | MS.gene45118 | PPI |
| MS.gene68292 | MS.gene027540 | PPI |
| MS.gene45116 | MS.gene68292 | PPI |
| MS.gene68293 | MS.gene68292 | PPI |
| MS.gene68292 | MS.gene042700 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68292.t1 | MTR_5g035750 | 89.513 | 267 | 26 | 1 | 2 | 268 | 123 | 387 | 1.05e-178 | 497 |
| MS.gene68292.t1 | MTR_5g035690 | 88.806 | 268 | 28 | 1 | 1 | 268 | 121 | 386 | 5.65e-178 | 495 |
| MS.gene68292.t1 | MTR_5g035665 | 87.640 | 267 | 31 | 1 | 2 | 268 | 115 | 379 | 1.45e-175 | 489 |
| MS.gene68292.t1 | MTR_5g035670 | 82.772 | 267 | 46 | 0 | 2 | 268 | 115 | 381 | 7.99e-164 | 459 |
| MS.gene68292.t1 | MTR_5g035760 | 75.746 | 268 | 65 | 0 | 1 | 268 | 113 | 380 | 4.84e-151 | 427 |
| MS.gene68292.t1 | MTR_6g472110 | 50.530 | 283 | 119 | 10 | 4 | 268 | 120 | 399 | 8.20e-82 | 251 |
| MS.gene68292.t1 | MTR_6g016660 | 43.262 | 282 | 138 | 8 | 6 | 268 | 120 | 398 | 1.41e-65 | 209 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68292.t1 | AT1G13480 | 42.294 | 279 | 140 | 8 | 1 | 268 | 119 | 387 | 2.32e-61 | 198 |
| MS.gene68292.t1 | AT1G13520 | 42.960 | 277 | 135 | 10 | 4 | 268 | 122 | 387 | 1.26e-60 | 196 |
| MS.gene68292.t1 | AT1G13510 | 40.876 | 274 | 144 | 7 | 1 | 268 | 119 | 380 | 2.69e-60 | 196 |
| MS.gene68292.t1 | AT1G13530 | 42.960 | 277 | 139 | 9 | 1 | 268 | 119 | 385 | 3.51e-60 | 195 |
| MS.gene68292.t1 | AT1G13490 | 42.960 | 277 | 138 | 9 | 2 | 268 | 116 | 382 | 1.51e-59 | 194 |
| MS.gene68292.t1 | AT1G13540 | 42.701 | 274 | 139 | 7 | 1 | 268 | 120 | 381 | 7.03e-59 | 192 |
| MS.gene68292.t1 | AT1G13470 | 43.431 | 274 | 139 | 8 | 1 | 268 | 115 | 378 | 3.60e-58 | 190 |
| MS.gene68292.t1 | AT1G13490 | 42.647 | 272 | 136 | 9 | 7 | 268 | 102 | 363 | 1.28e-56 | 186 |
| MS.gene68292.t1 | AT1G13500 | 41.935 | 279 | 140 | 10 | 2 | 268 | 118 | 386 | 2.48e-54 | 180 |
| MS.gene68292.t1 | AT1G61600 | 39.604 | 303 | 140 | 10 | 6 | 268 | 122 | 421 | 2.02e-52 | 176 |
| MS.gene68292.t1 | AT1G13550 | 39.350 | 277 | 146 | 9 | 1 | 265 | 119 | 385 | 1.29e-51 | 173 |
Find 52 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTGGAAAGTTGAAATT+TGG | 0.220039 | 5.2:-26259390 | None:intergenic |
| CCTTCCTTCACAAACATAAA+TGG | 0.225580 | 5.2:-26259456 | None:intergenic |
| AGCCTAAAGCTTGGCTTAAA+TGG | 0.235862 | 5.2:-26259341 | None:intergenic |
| GAGGAGAATGGATACAAAAT+TGG | 0.267080 | 5.2:+26259875 | MS.gene68292:CDS |
| GAAGGAAGGAATGAATTTAA+AGG | 0.316001 | 5.2:+26259470 | MS.gene68292:CDS |
| TTGAAGGAACGGACGCTGTT+TGG | 0.322013 | 5.2:+26259628 | MS.gene68292:CDS |
| ACAGACACTCACATCAAATT+AGG | 0.335727 | 5.2:+26259913 | MS.gene68292:CDS |
| TTGAAGGGCTCTAAAGCTAT+TGG | 0.344879 | 5.2:-26259186 | None:intergenic |
| CACATCAAATTAGGAGCAAA+TGG | 0.354134 | 5.2:+26259922 | MS.gene68292:CDS |
| TTGGCTTAAATGGTGGTTGC+GGG | 0.360622 | 5.2:-26259331 | None:intergenic |
| TCAAAGGAAAATGAAAATAG+TGG | 0.372912 | 5.2:+26259555 | MS.gene68292:CDS |
| AAGTTATTCTTTGAGGAGAA+TGG | 0.403630 | 5.2:+26259863 | MS.gene68292:CDS |
| AAATGGGAACAAAGAAGGTT+TGG | 0.414421 | 5.2:+26259741 | MS.gene68292:CDS |
| AGAAGGTTTGGATGGAATGT+TGG | 0.415149 | 5.2:+26259753 | MS.gene68292:CDS |
| TGAAGGAACGGACGCTGTTT+GGG | 0.429711 | 5.2:+26259629 | MS.gene68292:CDS |
| TAGTTGAAAGTTATTCTTTG+AGG | 0.431047 | 5.2:+26259856 | MS.gene68292:CDS |
| AGCTAACACTTGAGCAAAGA+TGG | 0.431951 | 5.2:+26259520 | MS.gene68292:CDS |
| CACCATTTAAGCCAAGCTTT+AGG | 0.434225 | 5.2:+26259339 | MS.gene68292:CDS |
| ACAGAAGTTGCTAATGTTGA+AGG | 0.436092 | 5.2:+26259612 | MS.gene68292:CDS |
| GAAGGTTTGGATGGAATGTT+GGG | 0.439478 | 5.2:+26259754 | MS.gene68292:CDS |
| GCCCTTCAATGATTATCAAC+AGG | 0.448229 | 5.2:+26259200 | MS.gene68292:CDS |
| GCCTCAGATGGAATTCCTCC+TGG | 0.453072 | 5.2:+26259267 | MS.gene68292:CDS |
| GATGAAAATAGATTGGTTGA+TGG | 0.465731 | 5.2:+26259651 | MS.gene68292:CDS |
| TACCTGTTGATAATCATTGA+AGG | 0.466990 | 5.2:-26259202 | None:intergenic |
| AAGTTGTTGAGGCGATGAAA+TGG | 0.484659 | 5.2:+26259724 | MS.gene68292:CDS |
| TGTTTGGGATGAAAATAGAT+TGG | 0.492208 | 5.2:+26259644 | MS.gene68292:CDS |
| TGTTCTGAATCAGTGATTGT+TGG | 0.495520 | 5.2:+26259420 | MS.gene68292:CDS |
| ACAAAAGTTGAGGAGTTTGA+AGG | 0.496121 | 5.2:+26259798 | MS.gene68292:CDS |
| CCATTTATGTTTGTGAAGGA+AGG | 0.497881 | 5.2:+26259456 | MS.gene68292:CDS |
| CTTGGCTTAAATGGTGGTTG+CGG | 0.507449 | 5.2:-26259332 | None:intergenic |
| GCAAAATCAGTTGCCTCAGA+TGG | 0.507539 | 5.2:+26259255 | MS.gene68292:CDS |
| GAATTCCTCCTGGATTATTG+AGG | 0.520488 | 5.2:+26259277 | MS.gene68292:CDS |
| GGGAACAAAGAAGGTTTGGA+TGG | 0.521569 | 5.2:+26259745 | MS.gene68292:CDS |
| TTTGGATGGAATGTTGGGAA+TGG | 0.530828 | 5.2:+26259759 | MS.gene68292:CDS |
| CGATGAAATGGGAACAAAGA+AGG | 0.534165 | 5.2:+26259736 | MS.gene68292:CDS |
| ACATCAAATTAGGAGCAAAT+GGG | 0.536534 | 5.2:+26259923 | MS.gene68292:CDS |
| GAAAATGAAAATAGTGGAGA+AGG | 0.537296 | 5.2:+26259561 | MS.gene68292:CDS |
| AGTTTGAAGGAGCAAATAAG+TGG | 0.542799 | 5.2:+26259811 | MS.gene68292:CDS |
| ATTTGGTAGCTTCGAACGCA+AGG | 0.544069 | 5.2:-26259373 | None:intergenic |
| ACCTGTTGATAATCATTGAA+GGG | 0.546054 | 5.2:-26259201 | None:intergenic |
| AGTTGTTGAGGCGATGAAAT+GGG | 0.564068 | 5.2:+26259725 | MS.gene68292:CDS |
| AATCATTTGAGCCTAAAGCT+TGG | 0.566503 | 5.2:-26259350 | None:intergenic |
| CTAAAGCTTGGCTTAAATGG+TGG | 0.584507 | 5.2:-26259338 | None:intergenic |
| TTGTCCATTTATGTTTGTGA+AGG | 0.585966 | 5.2:+26259452 | MS.gene68292:CDS |
| AATGAAAATAGTGGAGAAGG+TGG | 0.590819 | 5.2:+26259564 | MS.gene68292:CDS |
| AGTGAGAGTAACAAAAGTTG+AGG | 0.593305 | 5.2:+26259788 | MS.gene68292:CDS |
| TGGCTTAAATGGTGGTTGCG+GGG | 0.603595 | 5.2:-26259330 | None:intergenic |
| AGTTGCTAATGTTGAAGGAA+CGG | 0.611267 | 5.2:+26259617 | MS.gene68292:CDS |
| GCTAACACTTGAGCAAAGAT+GGG | 0.612705 | 5.2:+26259521 | MS.gene68292:CDS |
| TCCAGGAGGAATTCCATCTG+AGG | 0.618633 | 5.2:-26259268 | None:intergenic |
| AATCAGTGATTGTTGGAAAG+TGG | 0.623751 | 5.2:+26259427 | MS.gene68292:CDS |
| AAAACTACAGCAAAAGCATG+TGG | 0.702446 | 5.2:-26259148 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTGGAAAGTTGAAATT+TGG | - | chr5.2:26259393-26259412 | None:intergenic | 20.0% |
| !! | GAAATAATCAAAAAAGGTTA+TGG | + | chr5.2:26259225-26259244 | MS.gene68292:CDS | 20.0% |
| !! | TCAGAACAATCATTAAAAAT+TGG | - | chr5.2:26259408-26259427 | None:intergenic | 20.0% |
| ! | CAGGTAGAAATAATCAAAAA+AGG | + | chr5.2:26259219-26259238 | MS.gene68292:CDS | 25.0% |
| ! | TAGTTGAAAGTTATTCTTTG+AGG | + | chr5.2:26259856-26259875 | MS.gene68292:CDS | 25.0% |
| ! | TCAAAGGAAAATGAAAATAG+TGG | + | chr5.2:26259555-26259574 | MS.gene68292:CDS | 25.0% |
| ACATCAAATTAGGAGCAAAT+GGG | + | chr5.2:26259923-26259942 | MS.gene68292:CDS | 30.0% | |
| CTGGATTATTGAGGAAAAAA+AGG | + | chr5.2:26259286-26259305 | MS.gene68292:CDS | 30.0% | |
| GAAAAAAAGGTGGAAACTTA+AGG | + | chr5.2:26259299-26259318 | MS.gene68292:CDS | 30.0% | |
| GAAGGAAGGAATGAATTTAA+AGG | + | chr5.2:26259470-26259489 | MS.gene68292:CDS | 30.0% | |
| GATTATTGAGGAAAAAAAGG+TGG | + | chr5.2:26259289-26259308 | MS.gene68292:CDS | 30.0% | |
| TTGTCCATTTATGTTTGTGA+AGG | + | chr5.2:26259452-26259471 | MS.gene68292:CDS | 30.0% | |
| ! | AAGTTATTCTTTGAGGAGAA+TGG | + | chr5.2:26259863-26259882 | MS.gene68292:CDS | 30.0% |
| ! | GAAAATGAAAATAGTGGAGA+AGG | + | chr5.2:26259561-26259580 | MS.gene68292:CDS | 30.0% |
| ! | GATGAAAATAGATTGGTTGA+TGG | + | chr5.2:26259651-26259670 | MS.gene68292:CDS | 30.0% |
| ! | TGTTTGGGATGAAAATAGAT+TGG | + | chr5.2:26259644-26259663 | MS.gene68292:CDS | 30.0% |
| !! | ACCTGTTGATAATCATTGAA+GGG | - | chr5.2:26259204-26259223 | None:intergenic | 30.0% |
| !! | AGTGTTGATGAGAAAAATGT+TGG | + | chr5.2:26259690-26259709 | MS.gene68292:CDS | 30.0% |
| !! | TACCTGTTGATAATCATTGA+AGG | - | chr5.2:26259205-26259224 | None:intergenic | 30.0% |
| !!! | ATGGGAGAAGATTTTTTCAA+AGG | + | chr5.2:26259539-26259558 | MS.gene68292:CDS | 30.0% |
| !!! | ATTGAGTTTTGAAGTTGTTG+AGG | + | chr5.2:26259713-26259732 | MS.gene68292:CDS | 30.0% |
| !!! | CTTTTTTTCCTCAATAATCC+AGG | - | chr5.2:26259288-26259307 | None:intergenic | 30.0% |
| AAATGGGAACAAAGAAGGTT+TGG | + | chr5.2:26259741-26259760 | MS.gene68292:CDS | 35.0% | |
| AATCAGTGATTGTTGGAAAG+TGG | + | chr5.2:26259427-26259446 | MS.gene68292:CDS | 35.0% | |
| AATCATTTGAGCCTAAAGCT+TGG | - | chr5.2:26259353-26259372 | None:intergenic | 35.0% | |
| ACAAAAGTTGAGGAGTTTGA+AGG | + | chr5.2:26259798-26259817 | MS.gene68292:CDS | 35.0% | |
| ACAGAAGTTGCTAATGTTGA+AGG | + | chr5.2:26259612-26259631 | MS.gene68292:CDS | 35.0% | |
| ACAGACACTCACATCAAATT+AGG | + | chr5.2:26259913-26259932 | MS.gene68292:CDS | 35.0% | |
| AGTGAGAGTAACAAAAGTTG+AGG | + | chr5.2:26259788-26259807 | MS.gene68292:CDS | 35.0% | |
| AGTTGCTAATGTTGAAGGAA+CGG | + | chr5.2:26259617-26259636 | MS.gene68292:CDS | 35.0% | |
| AGTTTGAAGGAGCAAATAAG+TGG | + | chr5.2:26259811-26259830 | MS.gene68292:CDS | 35.0% | |
| CACATCAAATTAGGAGCAAA+TGG | + | chr5.2:26259922-26259941 | MS.gene68292:CDS | 35.0% | |
| CCATTTATGTTTGTGAAGGA+AGG | + | chr5.2:26259456-26259475 | MS.gene68292:CDS | 35.0% | |
| CCTTCCTTCACAAACATAAA+TGG | - | chr5.2:26259459-26259478 | None:intergenic | 35.0% | |
| GAGGAGAATGGATACAAAAT+TGG | + | chr5.2:26259875-26259894 | MS.gene68292:CDS | 35.0% | |
| ! | AAAACTACAGCAAAAGCATG+TGG | - | chr5.2:26259151-26259170 | None:intergenic | 35.0% |
| ! | AATGAAAATAGTGGAGAAGG+TGG | + | chr5.2:26259564-26259583 | MS.gene68292:CDS | 35.0% |
| ! | TGTTCTGAATCAGTGATTGT+TGG | + | chr5.2:26259420-26259439 | MS.gene68292:CDS | 35.0% |
| ! | TTTTTCCTCAATAATCCAGG+AGG | - | chr5.2:26259285-26259304 | None:intergenic | 35.0% |
| AAGTTGTTGAGGCGATGAAA+TGG | + | chr5.2:26259724-26259743 | MS.gene68292:CDS | 40.0% | |
| AGAAGGTTTGGATGGAATGT+TGG | + | chr5.2:26259753-26259772 | MS.gene68292:CDS | 40.0% | |
| AGCCTAAAGCTTGGCTTAAA+TGG | - | chr5.2:26259344-26259363 | None:intergenic | 40.0% | |
| AGCTAACACTTGAGCAAAGA+TGG | + | chr5.2:26259520-26259539 | MS.gene68292:CDS | 40.0% | |
| AGTTGTTGAGGCGATGAAAT+GGG | + | chr5.2:26259725-26259744 | MS.gene68292:CDS | 40.0% | |
| CACCATTTAAGCCAAGCTTT+AGG | + | chr5.2:26259339-26259358 | MS.gene68292:CDS | 40.0% | |
| CGATGAAATGGGAACAAAGA+AGG | + | chr5.2:26259736-26259755 | MS.gene68292:CDS | 40.0% | |
| CTAAAGCTTGGCTTAAATGG+TGG | - | chr5.2:26259341-26259360 | None:intergenic | 40.0% | |
| GAAGGTTTGGATGGAATGTT+GGG | + | chr5.2:26259754-26259773 | MS.gene68292:CDS | 40.0% | |
| GAATTCCTCCTGGATTATTG+AGG | + | chr5.2:26259277-26259296 | MS.gene68292:CDS | 40.0% | |
| GCCCTTCAATGATTATCAAC+AGG | + | chr5.2:26259200-26259219 | MS.gene68292:CDS | 40.0% | |
| GCTAACACTTGAGCAAAGAT+GGG | + | chr5.2:26259521-26259540 | MS.gene68292:CDS | 40.0% | |
| TTTGGATGGAATGTTGGGAA+TGG | + | chr5.2:26259759-26259778 | MS.gene68292:CDS | 40.0% | |
| !! | TTGAAGGGCTCTAAAGCTAT+TGG | - | chr5.2:26259189-26259208 | None:intergenic | 40.0% |
| !!! | GATTGGTTGATGGAGTTTTG+TGG | + | chr5.2:26259661-26259680 | MS.gene68292:CDS | 40.0% |
| CTTGGCTTAAATGGTGGTTG+CGG | - | chr5.2:26259335-26259354 | None:intergenic | 45.0% | |
| GCAAAATCAGTTGCCTCAGA+TGG | + | chr5.2:26259255-26259274 | MS.gene68292:CDS | 45.0% | |
| GGGAACAAAGAAGGTTTGGA+TGG | + | chr5.2:26259745-26259764 | MS.gene68292:CDS | 45.0% | |
| TTGGCTTAAATGGTGGTTGC+GGG | - | chr5.2:26259334-26259353 | None:intergenic | 45.0% | |
| ! | ATTTGGTAGCTTCGAACGCA+AGG | - | chr5.2:26259376-26259395 | None:intergenic | 45.0% |
| TCCAGGAGGAATTCCATCTG+AGG | - | chr5.2:26259271-26259290 | None:intergenic | 50.0% | |
| TGAAGGAACGGACGCTGTTT+GGG | + | chr5.2:26259629-26259648 | MS.gene68292:CDS | 50.0% | |
| TGGCTTAAATGGTGGTTGCG+GGG | - | chr5.2:26259333-26259352 | None:intergenic | 50.0% | |
| TTGAAGGAACGGACGCTGTT+TGG | + | chr5.2:26259628-26259647 | MS.gene68292:CDS | 50.0% | |
| GCCTCAGATGGAATTCCTCC+TGG | + | chr5.2:26259267-26259286 | MS.gene68292:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 26259144 | 26259950 | 26259144 | ID=MS.gene68292 |
| chr5.2 | mRNA | 26259144 | 26259950 | 26259144 | ID=MS.gene68292.t1;Parent=MS.gene68292 |
| chr5.2 | exon | 26259144 | 26259950 | 26259144 | ID=MS.gene68292.t1.exon1;Parent=MS.gene68292.t1 |
| chr5.2 | CDS | 26259144 | 26259950 | 26259144 | ID=cds.MS.gene68292.t1;Parent=MS.gene68292.t1 |
| Gene Sequence |
| Protein sequence |
