AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050494

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050494.t1 MTR_7g114470 94.372 462 25 1 1 461 1 462 0.0 914
MS.gene050494.t1 MTR_5g076700 87.146 459 59 0 3 461 2 460 0.0 852
MS.gene050494.t1 MTR_7g114560 77.996 459 100 1 3 461 2 459 0.0 765
MS.gene050494.t1 MTR_7g114550 76.790 461 105 2 1 461 1 459 0.0 755
MS.gene050494.t1 MTR_7g114520 75.541 462 112 1 1 461 1 462 0.0 739
MS.gene050494.t1 MTR_7g114490 76.443 433 101 1 3 435 2 433 0.0 712
MS.gene050494.t1 MTR_1g040195 57.516 459 164 6 7 461 5 436 0.0 530
MS.gene050494.t1 MTR_7g114420 81.356 295 28 1 167 461 1 268 4.64e-178 499
MS.gene050494.t1 MTR_1g040215 53.896 462 201 8 7 461 5 461 9.46e-178 506
MS.gene050494.t1 MTR_1g040175 54.248 459 201 6 7 461 5 458 5.05e-177 504
MS.gene050494.t1 MTR_1g081660 56.858 452 180 7 3 447 9 452 2.04e-175 499
MS.gene050494.t1 MTR_7g114510 70.701 314 92 0 148 461 7 320 9.78e-166 469
MS.gene050494.t1 MTR_1g040320 51.982 454 174 7 7 456 5 418 3.10e-162 465
MS.gene050494.t1 MTR_1g040315 51.092 458 176 7 7 461 5 417 2.46e-153 442
MS.gene050494.t1 MTR_0042s0190 51.916 287 132 5 167 450 1 284 4.35e-97 294
MS.gene050494.t1 MTR_0502s0010 54.918 244 102 4 102 342 1 239 1.23e-84 260
MS.gene050494.t1 MTR_7g114500 79.470 151 31 0 3 153 2 152 2.53e-83 253
MS.gene050494.t1 MTR_8g096530 35.733 389 236 8 9 392 5 384 9.32e-81 257
MS.gene050494.t1 MTR_3g098980 33.411 428 269 8 11 435 14 428 2.16e-79 253
MS.gene050494.t1 MTR_7g027175 35.052 388 238 8 14 396 19 397 2.31e-76 245
MS.gene050494.t1 MTR_0042s0190 52.727 220 100 4 234 450 1 219 7.50e-74 232
MS.gene050494.t1 MTR_3g051790 72.483 149 41 0 1 149 3 151 2.63e-73 229
MS.gene050494.t1 MTR_0042s0230 64.336 143 51 0 7 149 5 147 1.40e-64 205
MS.gene050494.t1 MTR_1g053315 30.505 436 284 12 19 439 26 457 3.12e-59 201
MS.gene050494.t1 MTR_8g039950 63.780 127 46 0 4 130 2 128 3.61e-57 185
MS.gene050494.t1 MTR_3g112180 31.378 392 243 11 21 400 22 399 6.54e-56 192
MS.gene050494.t1 MTR_2g105330 29.464 448 288 14 7 442 1 432 4.19e-55 189
MS.gene050494.t1 MTR_2g064495 29.241 448 289 14 7 442 1 432 5.48e-54 187
MS.gene050494.t1 MTR_4g055170 30.493 446 274 16 18 439 19 452 4.46e-52 182
MS.gene050494.t1 MTR_4g007540 29.356 419 258 14 43 442 34 433 1.00e-50 178
MS.gene050494.t1 MTR_8g075610 27.970 404 266 11 46 439 28 416 9.26e-46 164
MS.gene050494.t1 MTR_3g071320 29.500 400 262 10 10 396 4 396 4.49e-45 163
MS.gene050494.t1 MTR_2g015430 31.778 343 221 7 56 391 48 384 1.28e-43 159
MS.gene050494.t1 MTR_7g110610 27.696 408 215 11 7 400 1 342 6.15e-41 150
MS.gene050494.t1 MTR_3g035000 40.496 242 108 8 201 434 43 256 7.20e-41 148
MS.gene050494.t1 MTR_7g085930 27.952 415 253 13 18 394 13 419 1.44e-38 146
MS.gene050494.t1 MTR_7g085990 28.434 415 253 15 17 394 8 415 1.68e-38 145
MS.gene050494.t1 MTR_4g127650 27.938 451 279 14 19 439 18 452 3.03e-38 145
MS.gene050494.t1 MTR_7g084940 26.768 396 274 10 4 392 8 394 7.03e-38 143
MS.gene050494.t1 MTR_3g026950 25.747 435 258 12 19 415 10 417 3.00e-37 142
MS.gene050494.t1 MTR_7g084910 27.689 437 276 15 5 429 9 417 3.40e-37 141
MS.gene050494.t1 MTR_3g465930 27.342 395 260 12 18 391 17 405 8.81e-37 141
MS.gene050494.t1 MTR_4g097090 27.294 436 267 14 37 439 34 452 1.97e-36 140
MS.gene050494.t1 MTR_7g086000 24.894 470 287 18 19 443 12 460 1.82e-34 134
MS.gene050494.t1 MTR_3g465990 27.342 395 256 12 18 391 17 401 5.70e-34 133
MS.gene050494.t1 MTR_3g465980 27.848 395 254 12 18 391 17 401 5.45e-33 130
MS.gene050494.t1 MTR_3g049150 25.310 403 265 11 19 394 10 403 7.20e-33 130
MS.gene050494.t1 MTR_6g053810 27.723 404 249 15 65 439 70 459 1.07e-32 129
MS.gene050494.t1 MTR_3g465910 26.683 401 270 12 9 391 8 402 2.12e-32 129
MS.gene050494.t1 MTR_3g465880 27.983 461 277 18 18 439 17 461 2.30e-30 123
MS.gene050494.t1 MTR_5g090810 25.891 421 262 14 9 393 8 414 6.14e-30 122
MS.gene050494.t1 MTR_3g051820 66.304 92 29 2 371 461 1 91 5.33e-29 109
MS.gene050494.t1 MTR_5g090830 25.891 421 262 14 9 393 8 414 6.66e-29 119
MS.gene050494.t1 MTR_7g056087 25.000 444 288 14 18 437 11 433 9.85e-29 118
MS.gene050494.t1 MTR_3g465950 29.730 296 183 9 61 341 63 348 1.52e-27 114
MS.gene050494.t1 MTR_5g090880 26.277 411 252 16 63 438 64 458 3.87e-25 108
MS.gene050494.t1 MTR_3g070170 24.299 428 256 12 54 435 54 459 1.40e-24 106
MS.gene050494.t1 MTR_3g101960 26.097 433 269 16 25 432 30 436 4.49e-24 104
MS.gene050494.t1 MTR_5g026320 24.826 431 274 15 24 430 33 437 6.17e-24 104
MS.gene050494.t1 MTR_3g102020 26.526 426 275 17 26 432 31 437 8.74e-24 103
MS.gene050494.t1 MTR_3g101900 25.990 404 247 16 66 442 66 444 1.49e-22 100
MS.gene050494.t1 MTR_7g114417 70.149 67 16 2 79 144 20 83 1.63e-22 91.7
MS.gene050494.t1 MTR_7g114417 83.721 43 6 1 1 43 1 42 2.33e-14 68.6
MS.gene050494.t1 MTR_4g013050 33.735 166 96 5 19 180 12 167 5.82e-22 98.2
MS.gene050494.t1 MTR_3g450710 23.404 423 253 14 44 428 43 432 7.91e-22 98.2
MS.gene050494.t1 MTR_8g041770 24.812 399 263 13 25 405 31 410 3.33e-21 96.3
MS.gene050494.t1 MTR_3g086020 23.756 442 274 19 17 439 11 408 3.84e-21 95.5
MS.gene050494.t1 MTR_3g088420 24.594 431 258 19 25 428 25 415 5.85e-21 95.1
MS.gene050494.t1 MTR_3g465870 28.070 285 181 12 18 286 19 295 9.74e-21 94.7
MS.gene050494.t1 MTR_3g450690 23.192 401 240 13 63 428 69 436 1.34e-20 94.4
MS.gene050494.t1 MTR_3g102010 24.885 434 271 16 26 432 32 437 1.68e-20 94.0
MS.gene050494.t1 MTR_3g101930 25.558 403 243 18 38 405 44 424 1.74e-20 94.0
MS.gene050494.t1 MTR_5g084870 24.201 438 258 21 17 422 9 404 4.70e-20 92.8
MS.gene050494.t1 MTR_3g101970 24.943 437 282 15 25 446 30 435 6.83e-20 92.0
MS.gene050494.t1 MTR_4g022825 24.480 433 263 19 25 428 25 422 2.85e-19 90.1
MS.gene050494.t1 MTR_4g094842 24.582 419 249 16 25 405 28 417 1.43e-18 88.2
MS.gene050494.t1 MTR_4g088765 45.333 75 41 0 4 78 2 76 7.98e-18 79.7
MS.gene050494.t1 MTR_4g022720 23.218 435 271 16 25 433 25 422 1.19e-17 85.5
MS.gene050494.t1 MTR_1g094790 24.221 417 255 16 17 399 9 398 4.25e-17 83.6
MS.gene050494.t1 MTR_8g079710 23.721 430 270 19 25 428 30 427 4.82e-17 83.6
MS.gene050494.t1 MTR_4g094835 22.573 412 261 17 25 406 33 416 3.41e-15 77.8
MS.gene050494.t1 MTR_8g467580 23.716 409 262 19 17 399 9 393 3.99e-15 77.8
MS.gene050494.t1 MTR_4g022290 22.943 401 255 15 25 401 31 401 1.80e-14 75.5
MS.gene050494.t1 MTR_0190s0020 22.406 424 284 15 25 430 30 426 1.80e-14 75.5
MS.gene050494.t1 MTR_3g028210 22.222 432 273 15 46 435 46 456 1.62e-13 72.8
MS.gene050494.t1 MTR_4g094832 24.859 354 223 15 96 430 85 414 4.77e-13 71.2
MS.gene050494.t1 MTR_6g016815 29.452 146 92 6 61 198 64 206 4.91e-12 67.8
MS.gene050494.t1 MTR_5g084900 23.387 372 223 15 63 422 6 327 5.29e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050494.t1 AT5G17540 48.387 465 228 8 4 461 2 461 1.01e-148 432
MS.gene050494.t1 AT3G03480 47.427 447 225 7 2 444 10 450 4.17e-138 404
MS.gene050494.t1 AT3G48720 32.581 399 257 8 1 396 1 390 1.31e-75 243
MS.gene050494.t1 AT5G41040 35.190 395 236 8 11 396 33 416 1.38e-75 244
MS.gene050494.t1 AT5G41040 35.190 395 236 8 11 396 17 400 1.46e-75 243
MS.gene050494.t1 AT5G63560 35.533 394 230 9 11 396 9 386 1.75e-74 240
MS.gene050494.t1 AT1G27620 30.070 429 274 10 14 435 12 421 1.23e-58 199
MS.gene050494.t1 AT1G03390 29.978 447 289 14 9 441 23 459 8.46e-56 192
MS.gene050494.t1 AT5G48930 28.670 436 282 13 18 439 10 430 6.91e-49 173
MS.gene050494.t1 AT2G19070 29.630 459 278 14 10 439 5 447 1.11e-46 167
MS.gene050494.t1 AT3G62160 29.703 404 248 11 7 398 1 380 3.96e-46 166
MS.gene050494.t1 AT2G40230 29.250 400 257 12 11 396 5 392 3.70e-44 160
MS.gene050494.t1 AT5G57840 27.609 460 284 16 11 444 3 439 2.61e-42 155
MS.gene050494.t1 AT1G28680 30.189 371 238 12 32 391 27 387 1.10e-41 154
MS.gene050494.t1 AT5G07080 29.854 412 254 13 1 391 6 403 2.22e-41 153
MS.gene050494.t1 AT1G65450 27.412 456 301 15 4 443 8 449 1.16e-40 151
MS.gene050494.t1 AT3G47170 27.521 476 285 17 9 443 10 466 3.67e-39 147
MS.gene050494.t1 AT1G28680 29.891 368 230 13 32 381 27 384 5.02e-38 143
MS.gene050494.t1 AT2G25150 28.330 473 276 17 6 442 7 452 2.34e-35 137
MS.gene050494.t1 AT2G23510 28.916 415 246 17 3 391 7 398 2.38e-34 134
MS.gene050494.t1 AT1G31490 23.775 408 265 14 8 393 3 386 5.64e-26 110
MS.gene050494.t1 AT4G31910 26.226 469 300 20 6 441 2 457 1.42e-25 109
MS.gene050494.t1 AT5G42830 27.982 436 265 19 21 433 27 436 1.80e-25 108
MS.gene050494.t1 AT4G15390 25.225 444 278 20 17 435 14 428 1.96e-25 108
MS.gene050494.t1 AT4G15390 25.225 444 278 20 17 435 14 428 1.96e-25 108
MS.gene050494.t1 AT5G07860 25.570 395 243 15 63 433 69 436 6.89e-23 101
MS.gene050494.t1 AT3G50280 25.862 406 255 16 25 409 28 408 2.04e-22 99.8
MS.gene050494.t1 AT3G50270 25.121 414 267 13 25 417 30 421 3.82e-22 99.0
MS.gene050494.t1 AT3G26040 23.457 405 253 15 17 394 9 383 7.31e-22 98.2
MS.gene050494.t1 AT1G24430 25.629 437 274 18 17 435 11 414 8.28e-22 97.8
MS.gene050494.t1 AT1G78990 24.528 371 244 11 44 394 45 399 4.57e-21 95.9
MS.gene050494.t1 AT5G38130 25.679 405 257 15 25 410 48 427 4.65e-21 95.9
MS.gene050494.t1 AT5G07850 24.276 449 268 19 21 433 28 440 1.09e-20 94.7
MS.gene050494.t1 AT1G24420 25.390 449 264 18 17 435 11 418 1.27e-20 94.4
MS.gene050494.t1 AT1G24420 25.390 449 264 18 17 435 11 418 1.27e-20 94.4
MS.gene050494.t1 AT3G30280 24.208 442 278 19 17 432 12 422 1.07e-19 91.7
MS.gene050494.t1 AT1G32910 23.077 390 249 12 64 431 63 423 1.63e-19 91.3
MS.gene050494.t1 AT5G47980 25.670 448 277 18 15 436 9 426 2.53e-19 90.5
MS.gene050494.t1 AT1G65450 25.676 296 198 10 159 443 1 285 2.95e-19 88.2
MS.gene050494.t1 AT2G39980 23.628 419 254 12 66 432 62 466 7.01e-19 89.4
MS.gene050494.t1 AT5G23970 25.901 444 262 19 17 435 9 410 4.33e-18 86.7
MS.gene050494.t1 AT5G47950 23.678 435 272 19 17 432 12 405 1.21e-17 85.1
MS.gene050494.t1 AT5G16410 23.410 346 234 12 66 394 91 422 2.45e-17 84.7
MS.gene050494.t1 AT3G50300 23.784 370 244 12 63 410 56 409 8.97e-16 79.7
MS.gene050494.t1 AT4G15400 26.577 444 270 20 17 438 11 420 2.07e-15 78.6
MS.gene050494.t1 AT5G23940 24.331 411 265 16 53 437 53 443 1.65e-14 75.9
MS.gene050494.t1 AT3G50280 24.113 282 189 10 140 409 10 278 1.22e-12 68.9

Find 126 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTATCACTGCATGTTAT+TGG 0.178748 7.2:-2970028 MS.gene050494:CDS
CACTCCTTGTAGGATGATTT+TGG 0.205974 7.2:+2969484 None:intergenic
TCTTCCTTGATGTACGTATT+TGG 0.224399 7.2:+2970155 None:intergenic
GATATAACACTTGATCAATT+TGG 0.295091 7.2:-2971517 MS.gene050494:CDS
TTGTTGCTTGTCGGATATTA+TGG 0.296854 7.2:-2969912 MS.gene050494:CDS
GCATGTTTAAGAACCTTAAC+AGG 0.298889 7.2:+2971658 None:intergenic
ACAAACCTCTTCATATCTTC+AGG 0.311975 7.2:+2969534 None:intergenic
AACTCAACTGCATAACCTAA+TGG 0.312827 7.2:+2969834 None:intergenic
GGGTCTTTCTCTTTCATTGA+TGG 0.316385 7.2:+2971679 None:intergenic
CCATTCAACCTGTATGGAAT+AGG 0.323735 7.2:-2970247 MS.gene050494:CDS
CTGCCGCACAAAAGCATTGC+AGG 0.340219 7.2:-2970003 MS.gene050494:CDS
GAACGTCGTATAGAATTTCT+TGG 0.347222 7.2:+2971464 None:intergenic
TGGTAATTGTATTGCTTTCT+CGG 0.356387 7.2:-2969892 MS.gene050494:CDS
AGAGGAGTACATGCATTCTT+TGG 0.363021 7.2:-2969784 MS.gene050494:CDS
ATCCAGCTGCAGGAAGGATT+AGG 0.365308 7.2:-2971609 MS.gene050494:CDS
ATCACAAGCTACAAACTATA+AGG 0.369159 7.2:+2969440 None:intergenic
GAGCTTTGTGGAAACCCATT+AGG 0.375078 7.2:-2969849 MS.gene050494:CDS
CGTGACTGCATGGGCAGAAA+TGG 0.385863 7.2:-2970291 MS.gene050494:CDS
AAGTTCTCAGAAGTAAATTT+CGG 0.389814 7.2:-2969684 MS.gene050494:CDS
TCCAGCTGCAGGAAGGATTA+GGG 0.391602 7.2:-2971608 MS.gene050494:CDS
TCCCTAATCCTTCCTGCAGC+TGG 0.402307 7.2:+2971607 None:intergenic
AAACTAGGAGCACTCCTTGT+AGG 0.407155 7.2:+2969474 None:intergenic
CATTCAACCTGTATGGAATA+GGG 0.408225 7.2:-2970246 MS.gene050494:CDS
GCTAACCCTTTGTTGCTTGT+CGG 0.409807 7.2:-2969921 MS.gene050494:CDS
GTGTATGGTGGAGCAGTAAA+AGG 0.414611 7.2:-2969648 MS.gene050494:CDS
GGAGCAGTAAAAGGTGGAAT+TGG 0.418235 7.2:-2969639 MS.gene050494:CDS
GGGTGCTGGGCGAAAGTTGA+TGG 0.420210 7.2:-2971584 MS.gene050494:CDS
AACCTTCCATTCAACCTGTA+TGG 0.427456 7.2:-2970253 MS.gene050494:CDS
TCATGAGGAGTGGGTGCAGC+TGG 0.430177 7.2:+2971781 None:intergenic
CAGCTCCATCGCCCATTGTA+TGG 0.433098 7.2:+2970327 None:intergenic
GGAAGGATTAGGGAGGGTGC+TGG 0.433324 7.2:-2971598 MS.gene050494:CDS
ACTCAACTGCATAACCTAAT+GGG 0.436624 7.2:+2969835 None:intergenic
GAAAGATGTTTATTTACAAA+GGG 0.441292 7.2:-2969741 MS.gene050494:CDS
GAAATTCTATACGACGTTCC+AGG 0.442306 7.2:-2971460 MS.gene050494:CDS
CTGTGAAGAAGAAAGATTGA+TGG 0.446115 7.2:+2970114 None:intergenic
GGAAAGATGTTTATTTACAA+AGG 0.447433 7.2:-2969742 MS.gene050494:CDS
CCTATTCCATACAGGTTGAA+TGG 0.453015 7.2:+2970247 None:intergenic
ATCATTCGTACCTCTTCATC+TGG 0.459290 7.2:+2969975 None:intergenic
AAGAATGCAAAAGGAGAAGA+AGG 0.460427 7.2:-2969582 MS.gene050494:CDS
CATAGCTGCGATTCGTCGCC+TGG 0.472547 7.2:-2970084 MS.gene050494:CDS
TACAAAGGGAAGAACTTGTA+TGG 0.478317 7.2:-2969727 MS.gene050494:CDS
AGAATCTCCCTATTCCATAC+AGG 0.480630 7.2:+2970239 None:intergenic
ATGGTGGATTGCACTGGGGA+GGG 0.483447 7.2:-2971565 MS.gene050494:CDS
CAAACCTCTTCATATCTTCA+GGG 0.484202 7.2:+2969535 None:intergenic
GCATCGTTAATGCACGTTCG+AGG 0.486409 7.2:-2969950 MS.gene050494:CDS
TCAATAATATATTCTGATCC+TGG 0.488870 7.2:+2971442 None:intergenic
TCCTTGATGTACGTATTTGG+CGG 0.490692 7.2:+2970158 None:intergenic
AAGACCTGTATGAGTCGAAC+GGG 0.490760 7.2:+2971415 None:intergenic
CAAGTGTGGTGGTTTCATAA+TGG 0.491746 7.2:-2970363 MS.gene050494:CDS
GAACTTGTATGGTGTCAGAT+TGG 0.493406 7.2:-2969716 MS.gene050494:CDS
GTTTATTACTATCCAGCTGC+AGG 0.495084 7.2:-2971619 MS.gene050494:CDS
TGGAAGTACCAACTCTGGTT+GGG 0.497528 7.2:+2971801 None:intergenic
ATTCTTATGCGGAACAAGAA+AGG 0.498417 7.2:+2969599 None:intergenic
GGATGGGGTGAAGCTGTGTA+TGG 0.499977 7.2:-2969663 MS.gene050494:CDS
ATGGAATAGGGAGATTCTAA+TGG 0.500842 7.2:-2970234 MS.gene050494:CDS
GAGAAAGGTGAAGTGGAACC+AGG 0.501342 7.2:+2970066 None:intergenic
CATACAATGGGCGATGGAGC+TGG 0.513365 7.2:-2970326 MS.gene050494:CDS
ATTTCTTGGAAGCAAGGGAA+TGG 0.515708 7.2:+2971478 None:intergenic
GATGGTGGATTGCACTGGGG+AGG 0.517131 7.2:-2971566 MS.gene050494:CDS
GTTCCGCATAAGAATGCAAA+AGG 0.518570 7.2:-2969591 MS.gene050494:CDS
CTGGAAGTACCAACTCTGGT+TGG 0.519392 7.2:+2971800 None:intergenic
TATAGAATTTCTTGGAAGCA+AGG 0.520340 7.2:+2971472 None:intergenic
TTCCATACAGGTTGAATGGA+AGG 0.520405 7.2:+2970251 None:intergenic
GAAGGATTAGGGAGGGTGCT+GGG 0.522109 7.2:-2971597 MS.gene050494:CDS
CGTATGCAGTGCATTGAGAA+AGG 0.526515 7.2:+2970051 None:intergenic
AGTTTAACTTCATGAGGAGT+GGG 0.527780 7.2:+2971772 None:intergenic
AAGTTGATGGTGGATTGCAC+TGG 0.528428 7.2:-2971571 MS.gene050494:CDS
GAAAGAGAAAGACCCTGTTA+AGG 0.531454 7.2:-2971671 MS.gene050494:CDS
GGTTGCAAGTAACGTGTGGC+GGG 0.531576 7.2:+2970204 None:intergenic
CCGTCCCGTTCGACTCATAC+AGG 0.533062 7.2:-2971419 MS.gene050494:intron
CTCCGCACTGTGAACAATAG+TGG 0.533503 7.2:+2971826 None:intergenic
CTCAGAAGTAAATTTCGGAT+GGG 0.534991 7.2:-2969679 MS.gene050494:CDS
TCTCAGAAGTAAATTTCGGA+TGG 0.535849 7.2:-2969680 MS.gene050494:CDS
TGAGACAATTCGTGACTGCA+TGG 0.535891 7.2:-2970301 MS.gene050494:CDS
AAAGAGCTAAATGATATGCT+TGG 0.536007 7.2:-2969510 MS.gene050494:CDS
TTGAGCTTGAACCATACAAT+GGG 0.537449 7.2:-2970338 MS.gene050494:CDS
ACCACTGCAAAGGAGCTTTG+TGG 0.545376 7.2:-2969861 MS.gene050494:CDS
CCGCACAAAAGCATTGCAGG+TGG 0.552363 7.2:-2970000 MS.gene050494:CDS
GAGACAATTCGTGACTGCAT+GGG 0.553285 7.2:-2970300 MS.gene050494:CDS
AATATCCGACAAGCAACAAA+GGG 0.562922 7.2:+2969916 None:intergenic
GAGTTTAACTTCATGAGGAG+TGG 0.565045 7.2:+2971771 None:intergenic
TCCACAAAGCTCCTTTGCAG+TGG 0.567433 7.2:+2969860 None:intergenic
CATTGTATGGTTCAAGCTCA+AGG 0.569460 7.2:+2970340 None:intergenic
AAGGTAGACAAGATGAAACT+AGG 0.571958 7.2:+2969459 None:intergenic
ATAGAATTTCTTGGAAGCAA+GGG 0.573708 7.2:+2971473 None:intergenic
GGCAGCAGTCACCACTGCAA+AGG 0.576150 7.2:-2969871 MS.gene050494:CDS
TCAGAAGTAAATTTCGGATG+GGG 0.577426 7.2:-2969678 MS.gene050494:CDS
GCAGCTGGAAGTACCAACTC+TGG 0.584510 7.2:+2971796 None:intergenic
CTTGAGCTTGAACCATACAA+TGG 0.589328 7.2:-2970339 MS.gene050494:CDS
AGCAAAAGCTCAAGTTACAG+AGG 0.589925 7.2:-2969802 MS.gene050494:CDS
TAATATCCGACAAGCAACAA+AGG 0.591430 7.2:+2969915 None:intergenic
GCAGGTGGAGCCAGATGAAG+AGG 0.592868 7.2:-2969985 MS.gene050494:CDS
TCTTGGAAGCAAGGGAATGG+AGG 0.599063 7.2:+2971481 None:intergenic
AGTTGATGGTGGATTGCACT+GGG 0.599197 7.2:-2971570 MS.gene050494:CDS
GAGAGGAGTTTAACTTCATG+AGG 0.599961 7.2:+2971766 None:intergenic
TGGGGTGAAGCTGTGTATGG+TGG 0.601746 7.2:-2969660 MS.gene050494:CDS
TGACGAAAGACAAATATGAC+AGG 0.607837 7.2:+2971706 None:intergenic
ATTACTATCCAGCTGCAGGA+AGG 0.614273 7.2:-2971615 MS.gene050494:CDS
GCGGAGGTCCCAACCAGAGT+TGG 0.615833 7.2:-2971809 MS.gene050494:CDS
TGACAGGAAGATTGAAACGA+AGG 0.615959 7.2:+2971722 None:intergenic
TAAGACCTGTATGAGTCGAA+CGG 0.619194 7.2:+2971414 None:intergenic
GTGCATTGAGAAAGGTGAAG+TGG 0.620215 7.2:+2970059 None:intergenic
TCCGCCAAATACGTACATCA+AGG 0.620796 7.2:-2970159 MS.gene050494:CDS
GCCTTCTTGATCATCTATGT+CGG 0.622425 7.2:+2971744 None:intergenic
GTAACCAAAATCATCCTACA+AGG 0.623110 7.2:-2969488 MS.gene050494:CDS
TGGTTGCAAGTAACGTGTGG+CGG 0.626899 7.2:+2970203 None:intergenic
TTGAACCATACAATGGGCGA+TGG 0.631288 7.2:-2970332 MS.gene050494:CDS
TGCTGGGCGAAAGTTGATGG+TGG 0.635555 7.2:-2971581 MS.gene050494:CDS
CCTGTATGAGTCGAACGGGA+CGG 0.638129 7.2:+2971419 None:intergenic
TCCGACATAGATGATCAAGA+AGG 0.646509 7.2:-2971745 MS.gene050494:CDS
AAACCTCTTCATATCTTCAG+GGG 0.652653 7.2:+2969536 None:intergenic
TATGGTGGAGCAGTAAAAGG+TGG 0.653295 7.2:-2969645 MS.gene050494:CDS
CATGTTTAAGAACCTTAACA+GGG 0.656931 7.2:+2971659 None:intergenic
AGCTGCAGGAAGGATTAGGG+AGG 0.663235 7.2:-2971605 MS.gene050494:CDS
AGAATGCAAAAGGAGAAGAA+GGG 0.663405 7.2:-2969581 MS.gene050494:CDS
GTTACACGTCTCAAGTGTGG+TGG 0.666120 7.2:-2970374 MS.gene050494:CDS
AACCTCTTCATATCTTCAGG+GGG 0.666136 7.2:+2969537 None:intergenic
CAGGTTACACGTCTCAAGTG+TGG 0.668116 7.2:-2970377 MS.gene050494:CDS
CGATGGTTGCAAGTAACGTG+TGG 0.669479 7.2:+2970200 None:intergenic
GTATTTGTTCGTATTCACGA+TGG 0.674672 7.2:+2970183 None:intergenic
CTTGATCATCTATGTCGGAG+AGG 0.675142 7.2:+2971749 None:intergenic
CACTATTGTTCACAGTGCGG+AGG 0.677341 7.2:-2971825 MS.gene050494:CDS
GCATGGGCAGAAATGGCTCG+AGG 0.681407 7.2:-2970284 MS.gene050494:CDS
GCTGCAGGAAGGATTAGGGA+GGG 0.683826 7.2:-2971604 MS.gene050494:CDS
CACCACTATTGTTCACAGTG+CGG 0.732056 7.2:-2971828 MS.gene050494:CDS
GTTGATGGTGGATTGCACTG+GGG 0.762765 7.2:-2971569 MS.gene050494:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTAATTAATATAAATTCAT+AGG + chr7.2:2970398-2970417 None:intergenic 10.0%
!! AAATAATTGCATTATATAAG+TGG + chr7.2:2970339-2970358 None:intergenic 15.0%
!! TGATCAATAATTAAATTTCA+AGG + chr7.2:2969926-2969945 None:intergenic 15.0%
!!! TATATAATTTATTTGTTGTG+TGG + chr7.2:2970163-2970182 None:intergenic 15.0%
!! ACTTAAAAATAGTGTAAAGT+TGG + chr7.2:2970612-2970631 None:intergenic 20.0%
!! GAAAGATGTTTATTTACAAA+GGG - chr7.2:2971555-2971574 MS.gene050494:CDS 20.0%
!! GATAATTAGAATTAAATGAC+AGG - chr7.2:2970900-2970919 MS.gene050494:intron 20.0%
!! TAACATATACATACATCTTT+AGG - chr7.2:2970867-2970886 MS.gene050494:intron 20.0%
!! TTATCCCAAAAAAAAAATGA+GGG + chr7.2:2970557-2970576 None:intergenic 20.0%
!! TTTATCCCAAAAAAAAAATG+AGG + chr7.2:2970558-2970577 None:intergenic 20.0%
!!! AAACATCCTTGAAATTTTAT+AGG - chr7.2:2969951-2969970 MS.gene050494:CDS 20.0%
!!! ACCATTTTTCGTAAAAAAAT+TGG + chr7.2:2970583-2970602 None:intergenic 20.0%
!!! TTTATTCCAAGTCTAAAATT+CGG + chr7.2:2970092-2970111 None:intergenic 20.0%
! AAAATGATTGTAATGAGATC+AGG - chr7.2:2970023-2970042 MS.gene050494:CDS 25.0%
! AAATGATTGTAATGAGATCA+GGG - chr7.2:2970024-2970043 MS.gene050494:CDS 25.0%
! AAGAGACCTATAAAATTTCA+AGG + chr7.2:2969960-2969979 None:intergenic 25.0%
! AAGTTCTCAGAAGTAAATTT+CGG - chr7.2:2971612-2971631 MS.gene050494:CDS 25.0%
! ATTTCACATTAAATATGCCT+TGG + chr7.2:2970714-2970733 None:intergenic 25.0%
! GATATAACACTTGATCAATT+TGG - chr7.2:2969779-2969798 MS.gene050494:CDS 25.0%
! GGAAAGATGTTTATTTACAA+AGG - chr7.2:2971554-2971573 MS.gene050494:CDS 25.0%
! TATCCCAAAAAAAAAATGAG+GGG + chr7.2:2970556-2970575 None:intergenic 25.0%
! TCAATAATATATTCTGATCC+TGG + chr7.2:2969857-2969876 None:intergenic 25.0%
! TTTCACATTAAATATGCCTT+GGG + chr7.2:2970713-2970732 None:intergenic 25.0%
!! ATTATCATGCATTAACTTGA+TGG + chr7.2:2970502-2970521 None:intergenic 25.0%
!! GAATTTTAGACTTGGAATAA+AGG - chr7.2:2970091-2970110 MS.gene050494:CDS 25.0%
!! TGATCTCATTACAATCATTT+TGG + chr7.2:2970024-2970043 None:intergenic 25.0%
!! TTATTCCAAGTCTAAAATTC+GGG + chr7.2:2970091-2970110 None:intergenic 25.0%
!!! CTATTAGTCTTTGAAGTTAT+CGG + chr7.2:2970653-2970672 None:intergenic 25.0%
!!! GCCAATTTTTTTACGAAAAA+TGG - chr7.2:2970579-2970598 MS.gene050494:intron 25.0%
!!! TATTAGTCTTTGAAGTTATC+GGG + chr7.2:2970652-2970671 None:intergenic 25.0%
!!! TCAATGACATTAGTCTTTTT+AGG + chr7.2:2970824-2970843 None:intergenic 25.0%
AAAGAGCTAAATGATATGCT+TGG - chr7.2:2971786-2971805 MS.gene050494:CDS 30.0%
ACTTTATGATGCTTACAACA+TGG - chr7.2:2970244-2970263 MS.gene050494:CDS 30.0%
ATAGAATTTCTTGGAAGCAA+GGG + chr7.2:2969826-2969845 None:intergenic 30.0%
ATCTTATCACTGCATGTTAT+TGG - chr7.2:2971268-2971287 MS.gene050494:intron 30.0%
CATGTTTAAGAACCTTAACA+GGG + chr7.2:2969640-2969659 None:intergenic 30.0%
CTAAAGCGAACTATAATATG+AGG + chr7.2:2970435-2970454 None:intergenic 30.0%
CTCATATTATAGTTCGCTTT+AGG - chr7.2:2970433-2970452 MS.gene050494:intron 30.0%
CTTTATGATGCTTACAACAT+GGG - chr7.2:2970245-2970264 MS.gene050494:CDS 30.0%
TATAGAATTTCTTGGAAGCA+AGG + chr7.2:2969827-2969846 None:intergenic 30.0%
TGGTAATTGTATTGCTTTCT+CGG - chr7.2:2971404-2971423 MS.gene050494:intron 30.0%
! GGCAGATTTTATGGTTATTA+AGG - chr7.2:2971533-2971552 MS.gene050494:CDS 30.0%
! TATTCCAAGTCTAAAATTCG+GGG + chr7.2:2970090-2970109 None:intergenic 30.0%
!! TAATAGCTTGTTTGGAATAG+CGG - chr7.2:2970667-2970686 MS.gene050494:intron 30.0%
!! TCAAAGACTAATAGCTTGTT+TGG - chr7.2:2970659-2970678 MS.gene050494:intron 30.0%
!!! ACATAGTTTTGTCTATCATG+AGG + chr7.2:2970117-2970136 None:intergenic 30.0%
AAACCTCTTCATATCTTCAG+GGG + chr7.2:2971763-2971782 None:intergenic 35.0%
AACTCAACTGCATAACCTAA+TGG + chr7.2:2971465-2971484 None:intergenic 35.0%
AAGAATGCAAAAGGAGAAGA+AGG - chr7.2:2971714-2971733 MS.gene050494:CDS 35.0%
AAGGTAGACAAGATGAAACT+AGG + chr7.2:2971840-2971859 None:intergenic 35.0%
AATATCCGACAAGCAACAAA+GGG + chr7.2:2971383-2971402 None:intergenic 35.0%
ACAAACCTCTTCATATCTTC+AGG + chr7.2:2971765-2971784 None:intergenic 35.0%
ACTCAACTGCATAACCTAAT+GGG + chr7.2:2971464-2971483 None:intergenic 35.0%
AGAATGCAAAAGGAGAAGAA+GGG - chr7.2:2971715-2971734 MS.gene050494:CDS 35.0%
ATGGAATAGGGAGATTCTAA+TGG - chr7.2:2971062-2971081 MS.gene050494:intron 35.0%
ATTCTTATGCGGAACAAGAA+AGG + chr7.2:2971700-2971719 None:intergenic 35.0%
CAAACCTCTTCATATCTTCA+GGG + chr7.2:2971764-2971783 None:intergenic 35.0%
CATTCAACCTGTATGGAATA+GGG - chr7.2:2971050-2971069 MS.gene050494:intron 35.0%
CTCAGAAGTAAATTTCGGAT+GGG - chr7.2:2971617-2971636 MS.gene050494:CDS 35.0%
CTGTGAAGAAGAAAGATTGA+TGG + chr7.2:2971185-2971204 None:intergenic 35.0%
GAACGTCGTATAGAATTTCT+TGG + chr7.2:2969835-2969854 None:intergenic 35.0%
GCATGTTTAAGAACCTTAAC+AGG + chr7.2:2969641-2969660 None:intergenic 35.0%
GTAACCAAAATCATCCTACA+AGG - chr7.2:2971808-2971827 MS.gene050494:CDS 35.0%
GTATTTGTTCGTATTCACGA+TGG + chr7.2:2971116-2971135 None:intergenic 35.0%
GTGCATTGTAAAGTTGTATG+AGG + chr7.2:2970281-2970300 None:intergenic 35.0%
TAATATCCGACAAGCAACAA+AGG + chr7.2:2971384-2971403 None:intergenic 35.0%
TACAAAGGGAAGAACTTGTA+TGG - chr7.2:2971569-2971588 MS.gene050494:CDS 35.0%
TCAGAAGTAAATTTCGGATG+GGG - chr7.2:2971618-2971637 MS.gene050494:CDS 35.0%
TCTCAGAAGTAAATTTCGGA+TGG - chr7.2:2971616-2971635 MS.gene050494:CDS 35.0%
TCTTCCTTGATGTACGTATT+TGG + chr7.2:2971144-2971163 None:intergenic 35.0%
TGACGAAAGACAAATATGAC+AGG + chr7.2:2969593-2969612 None:intergenic 35.0%
TTGAGCTTGAACCATACAAT+GGG - chr7.2:2970958-2970977 MS.gene050494:intron 35.0%
TTTAGGCCTTAGAATGTGTT+AGG - chr7.2:2970450-2970469 MS.gene050494:intron 35.0%
! AAGTGTTGTAAGTGTAGCAT+TGG + chr7.2:2970316-2970335 None:intergenic 35.0%
! AGTTTAACTTCATGAGGAGT+GGG + chr7.2:2969527-2969546 None:intergenic 35.0%
! ATTCCAAGTCTAAAATTCGG+GGG + chr7.2:2970089-2970108 None:intergenic 35.0%
! GCATTCTTTGGCAGATTTTA+TGG - chr7.2:2971524-2971543 MS.gene050494:CDS 35.0%
! GTGGAATTGGATCTTTTCTT+GGG - chr7.2:2971670-2971689 MS.gene050494:CDS 35.0%
! TCTCCTTTTGCATTCTTATG+CGG + chr7.2:2971711-2971730 None:intergenic 35.0%
! TGGAATTGGATCTTTTCTTG+GGG - chr7.2:2971671-2971690 MS.gene050494:CDS 35.0%
! TTGTTGCTTGTCGGATATTA+TGG - chr7.2:2971384-2971403 MS.gene050494:intron 35.0%
!!! GGACCCCTCATTTTTTTTTT+GGG - chr7.2:2970550-2970569 MS.gene050494:intron 35.0%
!!! TGGACCCCTCATTTTTTTTT+TGG - chr7.2:2970549-2970568 MS.gene050494:intron 35.0%
AACCTCTTCATATCTTCAGG+GGG + chr7.2:2971762-2971781 None:intergenic 40.0%
AACCTTCCATTCAACCTGTA+TGG - chr7.2:2971043-2971062 MS.gene050494:intron 40.0%
AAGCTTTCTAACCAGTCCAA+CGG - chr7.2:2970526-2970545 MS.gene050494:intron 40.0%
AGAATCTCCCTATTCCATAC+AGG + chr7.2:2971060-2971079 None:intergenic 40.0%
AGCAAAAGCTCAAGTTACAG+AGG - chr7.2:2971494-2971513 MS.gene050494:CDS 40.0%
ATCATTCGTACCTCTTCATC+TGG + chr7.2:2971324-2971343 None:intergenic 40.0%
ATTTCTTGGAAGCAAGGGAA+TGG + chr7.2:2969821-2969840 None:intergenic 40.0%
CAAGTGTGGTGGTTTCATAA+TGG - chr7.2:2970933-2970952 MS.gene050494:intron 40.0%
CATTGTATGGTTCAAGCTCA+AGG + chr7.2:2970959-2970978 None:intergenic 40.0%
CCATTCAACCTGTATGGAAT+AGG - chr7.2:2971049-2971068 MS.gene050494:intron 40.0%
CTTGAGCTTGAACCATACAA+TGG - chr7.2:2970957-2970976 MS.gene050494:intron 40.0%
CTTTCCATCTCATTCAGTGT+CGG + chr7.2:2970476-2970495 None:intergenic 40.0%
GAAAGAGAAAGACCCTGTTA+AGG - chr7.2:2969625-2969644 MS.gene050494:CDS 40.0%
GAAATTCTATACGACGTTCC+AGG - chr7.2:2969836-2969855 MS.gene050494:CDS 40.0%
GCCTTCTTGATCATCTATGT+CGG + chr7.2:2969555-2969574 None:intergenic 40.0%
GGGTCTTTCTCTTTCATTGA+TGG + chr7.2:2969620-2969639 None:intergenic 40.0%
GTTCCGCATAAGAATGCAAA+AGG - chr7.2:2971705-2971724 MS.gene050494:CDS 40.0%
GTTTATTACTATCCAGCTGC+AGG - chr7.2:2969677-2969696 MS.gene050494:CDS 40.0%
TAAGACCTGTATGAGTCGAA+CGG + chr7.2:2969885-2969904 None:intergenic 40.0%
TCCGACATAGATGATCAAGA+AGG - chr7.2:2969551-2969570 MS.gene050494:CDS 40.0%
TCCTTGATGTACGTATTTGG+CGG + chr7.2:2971141-2971160 None:intergenic 40.0%
TGACAGGAAGATTGAAACGA+AGG + chr7.2:2969577-2969596 None:intergenic 40.0%
! AGAGGAGTACATGCATTCTT+TGG - chr7.2:2971512-2971531 MS.gene050494:CDS 40.0%
! CACTCCTTGTAGGATGATTT+TGG + chr7.2:2971815-2971834 None:intergenic 40.0%
! CCTATTCCATACAGGTTGAA+TGG + chr7.2:2971052-2971071 None:intergenic 40.0%
! GAACTTGTATGGTGTCAGAT+TGG - chr7.2:2971580-2971599 MS.gene050494:CDS 40.0%
! GAGAGGAGTTTAACTTCATG+AGG + chr7.2:2969533-2969552 None:intergenic 40.0%
! GAGTTTAACTTCATGAGGAG+TGG + chr7.2:2969528-2969547 None:intergenic 40.0%
! GGAATTGGATCTTTTCTTGG+GGG - chr7.2:2971672-2971691 MS.gene050494:CDS 40.0%
! GGTGGAATTGGATCTTTTCT+TGG - chr7.2:2971669-2971688 MS.gene050494:CDS 40.0%
! TGTTGTAAGTGTAGCATTGG+CGG + chr7.2:2970313-2970332 None:intergenic 40.0%
! TTCCATACAGGTTGAATGGA+AGG + chr7.2:2971048-2971067 None:intergenic 40.0%
AAGACCTGTATGAGTCGAAC+GGG + chr7.2:2969884-2969903 None:intergenic 45.0%
ATTACTATCCAGCTGCAGGA+AGG - chr7.2:2969681-2969700 MS.gene050494:CDS 45.0%
CACCACTATTGTTCACAGTG+CGG - chr7.2:2969468-2969487 MS.gene050494:CDS 45.0%
CATTCACCGCTAAAACAGAC+AGG - chr7.2:2970783-2970802 MS.gene050494:intron 45.0%
CGTATGCAGTGCATTGAGAA+AGG + chr7.2:2971248-2971267 None:intergenic 45.0%
CTTGATCATCTATGTCGGAG+AGG + chr7.2:2969550-2969569 None:intergenic 45.0%
GAGACAATTCGTGACTGCAT+GGG - chr7.2:2970996-2971015 MS.gene050494:intron 45.0%
GATTGTAATGAGATCAGGGC+TGG - chr7.2:2970028-2970047 MS.gene050494:CDS 45.0%
GGAGCAGTAAAAGGTGGAAT+TGG - chr7.2:2971657-2971676 MS.gene050494:CDS 45.0%
GTGCATTGAGAAAGGTGAAG+TGG + chr7.2:2971240-2971259 None:intergenic 45.0%
TCCGCCAAATACGTACATCA+AGG - chr7.2:2971137-2971156 MS.gene050494:intron 45.0%
TGAGACAATTCGTGACTGCA+TGG - chr7.2:2970995-2971014 MS.gene050494:intron 45.0%
TGTCGGCCTAACACATTCTA+AGG + chr7.2:2970459-2970478 None:intergenic 45.0%
TTGAACCATACAATGGGCGA+TGG - chr7.2:2970964-2970983 MS.gene050494:intron 45.0%
! GAGCTTTGTGGAAACCCATT+AGG - chr7.2:2971447-2971466 MS.gene050494:CDS 45.0%
! GCTAACCCTTTGTTGCTTGT+CGG - chr7.2:2971375-2971394 MS.gene050494:intron 45.0%
! GTGTATGGTGGAGCAGTAAA+AGG - chr7.2:2971648-2971667 MS.gene050494:CDS 45.0%
! TATGGTGGAGCAGTAAAAGG+TGG - chr7.2:2971651-2971670 MS.gene050494:CDS 45.0%
!! AAACTAGGAGCACTCCTTGT+AGG + chr7.2:2971825-2971844 None:intergenic 45.0%
!! AAGTTGATGGTGGATTGCAC+TGG - chr7.2:2969725-2969744 MS.gene050494:CDS 45.0%
!! AGTTGATGGTGGATTGCACT+GGG - chr7.2:2969726-2969745 MS.gene050494:CDS 45.0%
!! GTAGCTCCTGTCTGTTTTAG+CGG + chr7.2:2970792-2970811 None:intergenic 45.0%
!! TGGAAGTACCAACTCTGGTT+GGG + chr7.2:2969498-2969517 None:intergenic 45.0%
!! TTTGCATTTCTAGCGTCCCA+AGG - chr7.2:2970694-2970713 MS.gene050494:intron 45.0%
AAAAATGAGGGGTCCACCGT+TGG + chr7.2:2970545-2970564 None:intergenic 50.0%
ACCACTGCAAAGGAGCTTTG+TGG - chr7.2:2971435-2971454 MS.gene050494:CDS 50.0%
ATCCAGCTGCAGGAAGGATT+AGG - chr7.2:2969687-2969706 MS.gene050494:CDS 50.0%
CACTATTGTTCACAGTGCGG+AGG - chr7.2:2969471-2969490 MS.gene050494:CDS 50.0%
CAGGTTACACGTCTCAAGTG+TGG - chr7.2:2970919-2970938 MS.gene050494:intron 50.0%
CGATGGTTGCAAGTAACGTG+TGG + chr7.2:2971099-2971118 None:intergenic 50.0%
CTCCGCACTGTGAACAATAG+TGG + chr7.2:2969473-2969492 None:intergenic 50.0%
CTTTCTAACCAGTCCAACGG+TGG - chr7.2:2970529-2970548 MS.gene050494:intron 50.0%
GAGAAAGGTGAAGTGGAACC+AGG + chr7.2:2971233-2971252 None:intergenic 50.0%
GCATCGTTAATGCACGTTCG+AGG - chr7.2:2971346-2971365 MS.gene050494:intron 50.0%
GTTACACGTCTCAAGTGTGG+TGG - chr7.2:2970922-2970941 MS.gene050494:intron 50.0%
TAGGCCGACACTGAATGAGA+TGG - chr7.2:2970469-2970488 MS.gene050494:intron 50.0%
TCCACAAAGCTCCTTTGCAG+TGG + chr7.2:2971439-2971458 None:intergenic 50.0%
TCCAGCTGCAGGAAGGATTA+GGG - chr7.2:2969688-2969707 MS.gene050494:CDS 50.0%
TCTTCAGGGGGCAAGCAAAT+TGG + chr7.2:2971750-2971769 None:intergenic 50.0%
TCTTGGAAGCAAGGGAATGG+AGG + chr7.2:2969818-2969837 None:intergenic 50.0%
TGCCCCCTGAAGATATGAAG+AGG - chr7.2:2971757-2971776 MS.gene050494:CDS 50.0%
TGGTTGCAAGTAACGTGTGG+CGG + chr7.2:2971096-2971115 None:intergenic 50.0%
! AGGCCCCCGAATTTTAGACT+TGG - chr7.2:2970083-2970102 MS.gene050494:CDS 50.0%
! CCACCTGCAATGCTTTTGTG+CGG + chr7.2:2971299-2971318 None:intergenic 50.0%
!! CTGGAAGTACCAACTCTGGT+TGG + chr7.2:2969499-2969518 None:intergenic 50.0%
!! GTTGATGGTGGATTGCACTG+GGG - chr7.2:2969727-2969746 MS.gene050494:CDS 50.0%
!! GTTTGGGTTGCCTTACCCTT+AGG + chr7.2:2970054-2970073 None:intergenic 50.0%
AAATTCGGGGGCCTAAAGCG+AGG + chr7.2:2970077-2970096 None:intergenic 55.0%
AATTCGGGGGCCTAAAGCGA+GGG + chr7.2:2970076-2970095 None:intergenic 55.0%
AGCTGCAGGAAGGATTAGGG+AGG - chr7.2:2969691-2969710 MS.gene050494:CDS 55.0%
CAGCTCCATCGCCCATTGTA+TGG + chr7.2:2970972-2970991 None:intergenic 55.0%
CATACAATGGGCGATGGAGC+TGG - chr7.2:2970970-2970989 MS.gene050494:intron 55.0%
CCTGTATGAGTCGAACGGGA+CGG + chr7.2:2969880-2969899 None:intergenic 55.0%
CGTGACTGCATGGGCAGAAA+TGG - chr7.2:2971005-2971024 MS.gene050494:intron 55.0%
GCAACCCAAACCCTCGCTTT+AGG - chr7.2:2970063-2970082 MS.gene050494:CDS 55.0%
GCTGCAGGAAGGATTAGGGA+GGG - chr7.2:2969692-2969711 MS.gene050494:CDS 55.0%
GGATGGGGTGAAGCTGTGTA+TGG - chr7.2:2971633-2971652 MS.gene050494:CDS 55.0%
GGTTGCAAGTAACGTGTGGC+GGG + chr7.2:2971095-2971114 None:intergenic 55.0%
TCCCTAATCCTTCCTGCAGC+TGG + chr7.2:2969692-2969711 None:intergenic 55.0%
TGGGGTGAAGCTGTGTATGG+TGG - chr7.2:2971636-2971655 MS.gene050494:CDS 55.0%
!! ATGAGATCAGGGCTGGCCTA+AGG - chr7.2:2970035-2970054 MS.gene050494:CDS 55.0%
!! ATGGTGGATTGCACTGGGGA+GGG - chr7.2:2969731-2969750 MS.gene050494:CDS 55.0%
!! CCGCACAAAAGCATTGCAGG+TGG - chr7.2:2971296-2971315 MS.gene050494:intron 55.0%
!! CTGCCGCACAAAAGCATTGC+AGG - chr7.2:2971293-2971312 MS.gene050494:intron 55.0%
!! GAAGGATTAGGGAGGGTGCT+GGG - chr7.2:2969699-2969718 MS.gene050494:CDS 55.0%
!! GCAGCTGGAAGTACCAACTC+TGG + chr7.2:2969503-2969522 None:intergenic 55.0%
!! TGAGATCAGGGCTGGCCTAA+GGG - chr7.2:2970036-2970055 MS.gene050494:CDS 55.0%
!! TGCTGGGCGAAAGTTGATGG+TGG - chr7.2:2969715-2969734 MS.gene050494:CDS 55.0%
CATAGCTGCGATTCGTCGCC+TGG - chr7.2:2971212-2971231 MS.gene050494:intron 60.0%
CCGTCCCGTTCGACTCATAC+AGG - chr7.2:2969877-2969896 MS.gene050494:CDS 60.0%
GCAGGTGGAGCCAGATGAAG+AGG - chr7.2:2971311-2971330 MS.gene050494:intron 60.0%
GCATGGGCAGAAATGGCTCG+AGG - chr7.2:2971012-2971031 MS.gene050494:intron 60.0%
GGCAGCAGTCACCACTGCAA+AGG - chr7.2:2971425-2971444 MS.gene050494:CDS 60.0%
GGGGCCTAAAGCGAGGGTTT+GGG + chr7.2:2970070-2970089 None:intergenic 60.0%
TCATGAGGAGTGGGTGCAGC+TGG + chr7.2:2969518-2969537 None:intergenic 60.0%
! GGAAGGATTAGGGAGGGTGC+TGG - chr7.2:2969698-2969717 MS.gene050494:CDS 60.0%
!! GATGGTGGATTGCACTGGGG+AGG - chr7.2:2969730-2969749 MS.gene050494:CDS 60.0%
!! GGGTGCTGGGCGAAAGTTGA+TGG - chr7.2:2969712-2969731 MS.gene050494:CDS 60.0%
!! TCAGGGCTGGCCTAAGGGTA+AGG - chr7.2:2970041-2970060 MS.gene050494:CDS 60.0%
GCGGAGGTCCCAACCAGAGT+TGG - chr7.2:2969487-2969506 MS.gene050494:CDS 65.0%
GGGGGCCTAAAGCGAGGGTT+TGG + chr7.2:2970071-2970090 None:intergenic 65.0%
TGAGGGGTCCACCGTTGGAC+TGG + chr7.2:2970540-2970559 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 2969455 2971863 2969455 ID=MS.gene050494
chr7.2 mRNA 2969455 2971863 2969455 ID=MS.gene050494.t1;Parent=MS.gene050494
chr7.2 exon 2971420 2971863 2971420 ID=MS.gene050494.t1.exon1;Parent=MS.gene050494.t1
chr7.2 CDS 2971420 2971863 2971420 ID=cds.MS.gene050494.t1;Parent=MS.gene050494.t1
chr7.2 exon 2969455 2970396 2969455 ID=MS.gene050494.t1.exon2;Parent=MS.gene050494.t1
chr7.2 CDS 2969455 2970396 2969455 ID=cds.MS.gene050494.t1;Parent=MS.gene050494.t1
Gene Sequence

>MS.gene050494

ATGACTTCTTCATCACCACTATTGTTCACAGTGCGGAGGTCCCAACCAGAGTTGGTACTTCCAGCTGCACCCACTCCTCATGAAGTTAAACTCCTCTCCGACATAGATGATCAAGAAGGCCTTCGTTTCAATCTTCCTGTCATATTTGTCTTTCGTCATGAACCATCAATGAAAGAGAAAGACCCTGTTAAGGTTCTTAAACATGCACTCTCACAAACACTTGTTTATTACTATCCAGCTGCAGGAAGGATTAGGGAGGGTGCTGGGCGAAAGTTGATGGTGGATTGCACTGGGGAGGGTGTCATGTTCATTGAAGCTGAAGCTGATATAACACTTGATCAATTTGGTGATGCTCTGCAACCTCCATTCCCTTGCTTCCAAGAAATTCTATACGACGTTCCAGGATCAGAATATATTATTGACCGTCCCGTTCGACTCATACAGGTCTTATTATAATAATTCTCACATCCTTGAAATTTAATTATTGATCAAAACAAAACATCCTTGAAATTTTATAGGTCTCTTAACTACTATGTGACTTTAAAAATTTAATCTCTAGCGAAATTCCAAAATGATTGTAATGAGATCAGGGCTGGCCTAAGGGTAAGGCAACCCAAACCCTCGCTTTAGGCCCCCGAATTTTAGACTTGGAATAAAGGCCTCATGATAGACAAAACTATGTGTATATATACAAACGAATAAAAGCCACACAACAAATAAATTATATAAAGATTCAAAAAACAAATGAGTTATAAAACTTTGTCATGAAGCAAAGATTGTAGACGTGAGACTTTATGATGCTTACAACATGGGTTCAACACTTCCTCATACAACTTTACAATGCACACTATTTTTCCGCCAATGCTACACTTACAACACTTCCACTTATATAATGCAATTATTTATTTTTCTTTTTAGTTGTAATTTTTTTTTTATAAAACCTATGAATTTATATTAATTACACTCATTAAAAAAAGCCTCATATTATAGTTCGCTTTAGGCCTTAGAATGTGTTAGGCCGACACTGAATGAGATGGAAAGATTCCATCAAGTTAATGCATGATAATTTTCAAGCTTTCTAACCAGTCCAACGGTGGACCCCTCATTTTTTTTTTGGGATAAAAGCCAATTTTTTTACGAAAAATGGTAAATGTCCAACTTTACACTATTTTTAAGTGATTCATATTACATACACCCGATAACTTCAAAGACTAATAGCTTGTTTGGAATAGCGGTAGATTTGCATTTCTAGCGTCCCAAGGCATATTTAATGTGAAATTGAAGAATATACAATTTGTAGCTTTAGAAAAAAACGTACATTAAGCTTGCATTCACCGCTAAAACAGACAGGAGCTACATAGAACTACCTAAAAAGACTAATGTCATTGATTTGATTTTTTTTTACTAGTTTATAACATATACATACATCTTTAGGTTACACGCATGATAATTAGAATTAAATGACAGGTTACACGTCTCAAGTGTGGTGGTTTCATAATGGCCTTGAGCTTGAACCATACAATGGGCGATGGAGCTGGTTTGAGACAATTCGTGACTGCATGGGCAGAAATGGCTCGAGGAGCAAATCAACCTTCCATTCAACCTGTATGGAATAGGGAGATTCTAATGGCAAGAGACCCGCCACACGTTACTTGCAACCATCGTGAATACGAACAAATACTTCCGCCAAATACGTACATCAAGGAAGAAGATACTACTATTGTAGTCCATCAATCTTTCTTCTTCACAGCTGCTCACATAGCTGCGATTCGTCGCCTGGTTCCACTTCACCTTTCTCAATGCACTGCATACGATCTTATCACTGCATGTTATTGGTGCTGCCGCACAAAAGCATTGCAGGTGGAGCCAGATGAAGAGGTACGAATGATGTGCATCGTTAATGCACGTTCGAGGTTTAGTGCTAACCCTTTGTTGCTTGTCGGATATTATGGTAATTGTATTGCTTTCTCGGCAGCAGTCACCACTGCAAAGGAGCTTTGTGGAAACCCATTAGGTTATGCAGTTGAGTTGATAAGAAAAGCAAAAGCTCAAGTTACAGAGGAGTACATGCATTCTTTGGCAGATTTTATGGTTATTAAGGAAAGATGTTTATTTACAAAGGGAAGAACTTGTATGGTGTCAGATTGGACTCGTGCTAAGTTCTCAGAAGTAAATTTCGGATGGGGTGAAGCTGTGTATGGTGGAGCAGTAAAAGGTGGAATTGGATCTTTTCTTGGGGGAACCTTTCTTGTTCCGCATAAGAATGCAAAAGGAGAAGAAGGGTTAATATTACCAATTTGCTTGCCCCCTGAAGATATGAAGAGGTTTGTTAAAGAGCTAAATGATATGCTTGGTAACCAAAATCATCCTACAAGGAGTGCTCCTAGTTTCATCTTGTCTACCTTATAG
Protein sequence

>MS.gene050494.t1

MTSSSPLLFTVRRSQPELVLPAAPTPHEVKLLSDIDDQEGLRFNLPVIFVFRHEPSMKEKDPVKVLKHALSQTLVYYYPAAGRIREGAGRKLMVDCTGEGVMFIEAEADITLDQFGDALQPPFPCFQEILYDVPGSEYIIDRPVRLIQVTRLKCGGFIMALSLNHTMGDGAGLRQFVTAWAEMARGANQPSIQPVWNREILMARDPPHVTCNHREYEQILPPNTYIKEEDTTIVVHQSFFFTAAHIAAIRRLVPLHLSQCTAYDLITACYWCCRTKALQVEPDEEVRMMCIVNARSRFSANPLLLVGYYGNCIAFSAAVTTAKELCGNPLGYAVELIRKAKAQVTEEYMHSLADFMVIKERCLFTKGRTCMVSDWTRAKFSEVNFGWGEAVYGGAVKGGIGSFLGGTFLVPHKNAKGEEGLILPICLPPEDMKRFVKELNDMLGNQNHPTRSAPSFILSTL