AlfalfaGEDB | MS.gene50745

M. sativa cultivar XinJiangDaYe / MS.gene50745

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50745.t1	XP_003606980.1	91.7	156	13	0	1	156	1	156	5.40E-50	207.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50745.t1	Q9LZP8	51.8	85	41	0	17	101	12	96	1.8e-14	80.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50745.t1	G7JIJ4	91.7	156	13	0	1	156	1	156	3.9e-50	207.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene50745.t1	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene50745	0.811097	8.30E-51	-1.69E-46
MS.gene049378	MS.gene50745	0.802843	4.63E-49	-1.69E-46
MS.gene049590	MS.gene50745	0.802231	6.19E-49	-1.69E-46
MS.gene049647	MS.gene50745	0.800539	1.37E-48	-1.69E-46
MS.gene050105	MS.gene50745	0.847371	1.21E-59	-1.69E-46
MS.gene050156	MS.gene50745	0.803164	3.97E-49	-1.69E-46
MS.gene050826	MS.gene50745	0.849539	3.03E-60	-1.69E-46
MS.gene051122	MS.gene50745	0.809683	1.68E-50	-1.69E-46
MS.gene051190	MS.gene50745	0.811251	7.69E-51	-1.69E-46
MS.gene051332	MS.gene50745	0.816373	5.72E-52	-1.69E-46
MS.gene051368	MS.gene50745	0.817156	3.82E-52	-1.69E-46
MS.gene051800	MS.gene50745	0.804772	1.84E-49	-1.69E-46
MS.gene051924	MS.gene50745	-0.837915	3.92E-57	-1.69E-46
MS.gene051926	MS.gene50745	-0.823141	1.63E-53	-1.69E-46
MS.gene052190	MS.gene50745	0.826191	3.11E-54	-1.69E-46
MS.gene052333	MS.gene50745	0.853428	2.41E-61	-1.69E-46
MS.gene052389	MS.gene50745	0.825954	3.54E-54	-1.69E-46
MS.gene052534	MS.gene50745	0.804443	2.15E-49	-1.69E-46
MS.gene052586	MS.gene50745	0.815723	7.99E-52	-1.69E-46
MS.gene052674	MS.gene50745	0.847461	1.14E-59	-1.69E-46
MS.gene052675	MS.gene50745	0.849263	3.62E-60	-1.69E-46
MS.gene052677	MS.gene50745	0.846606	1.95E-59	-1.69E-46
MS.gene052773	MS.gene50745	0.803255	3.80E-49	-1.69E-46
MS.gene053166	MS.gene50745	0.833547	5.01E-56	-1.69E-46
MS.gene05415	MS.gene50745	0.814719	1.34E-51	-1.69E-46
MS.gene054291	MS.gene50745	-0.839167	1.86E-57	-1.69E-46
MS.gene054467	MS.gene50745	0.830164	3.43E-55	-1.69E-46
MS.gene054468	MS.gene50745	0.860418	2.09E-63	-1.69E-46
MS.gene054479	MS.gene50745	0.847732	9.60E-60	-1.69E-46
MS.gene055227	MS.gene50745	0.823209	1.57E-53	-1.69E-46
MS.gene055453	MS.gene50745	-0.828415	9.10E-55	-1.69E-46
MS.gene055455	MS.gene50745	-0.826298	2.93E-54	-1.69E-46
MS.gene055701	MS.gene50745	0.852855	3.51E-61	-1.69E-46
MS.gene055818	MS.gene50745	0.831014	2.12E-55	-1.69E-46
MS.gene055926	MS.gene50745	0.849179	3.82E-60	-1.69E-46
MS.gene05595	MS.gene50745	0.819053	1.42E-52	-1.69E-46
MS.gene056045	MS.gene50745	0.805731	1.16E-49	-1.69E-46
MS.gene05604	MS.gene50745	0.822561	2.22E-53	-1.69E-46
MS.gene056173	MS.gene50745	0.800667	1.29E-48	-1.69E-46
MS.gene056506	MS.gene50745	0.813675	2.27E-51	-1.69E-46
MS.gene056779	MS.gene50745	0.840757	7.17E-58	-1.69E-46
MS.gene057012	MS.gene50745	0.800496	1.40E-48	-1.69E-46
MS.gene057026	MS.gene50745	0.838256	3.20E-57	-1.69E-46
MS.gene057156	MS.gene50745	-0.823597	1.27E-53	-1.69E-46
MS.gene057598	MS.gene50745	0.812447	4.22E-51	-1.69E-46
MS.gene057855	MS.gene50745	0.801534	8.61E-49	-1.69E-46
MS.gene058223	MS.gene50745	-0.809969	1.45E-50	-1.69E-46
MS.gene058979	MS.gene50745	0.820721	5.90E-53	-1.69E-46
MS.gene059283	MS.gene50745	0.816736	4.74E-52	-1.69E-46
MS.gene059438	MS.gene50745	0.805953	1.04E-49	-1.69E-46
MS.gene059439	MS.gene50745	0.834483	2.92E-56	-1.69E-46
MS.gene059440	MS.gene50745	0.838668	2.51E-57	-1.69E-46
MS.gene059570	MS.gene50745	0.806927	6.49E-50	-1.69E-46
MS.gene059952	MS.gene50745	0.834811	2.42E-56	-1.69E-46
MS.gene060011	MS.gene50745	0.818213	2.20E-52	-1.69E-46
MS.gene060191	MS.gene50745	0.818068	2.38E-52	-1.69E-46
MS.gene060322	MS.gene50745	0.807579	4.72E-50	-1.69E-46
MS.gene06052	MS.gene50745	0.826689	2.36E-54	-1.69E-46
MS.gene060777	MS.gene50745	0.826912	2.09E-54	-1.69E-46
MS.gene060850	MS.gene50745	0.808961	2.39E-50	-1.69E-46
MS.gene060851	MS.gene50745	0.813135	2.98E-51	-1.69E-46
MS.gene060852	MS.gene50745	0.813454	2.54E-51	-1.69E-46
MS.gene060879	MS.gene50745	0.800972	1.12E-48	-1.69E-46
MS.gene060932	MS.gene50745	0.800707	1.27E-48	-1.69E-46
MS.gene060998	MS.gene50745	0.80572	1.17E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50745.t1	MTR_4g070860	96.795	156	5	0	1	156	1	156	2.92e-107	302
MS.gene50745.t1	MTR_1g022495	59.627	161	59	4	1	156	1	160	1.48e-60	184
MS.gene50745.t1	MTR_3g117120	65.605	157	43	2	1	153	1	150	3.94e-58	178
MS.gene50745.t1	MTR_8g086020	53.086	162	68	4	1	156	1	160	2.79e-48	153
MS.gene50745.t1	MTR_3g112220	69.620	79	24	0	27	105	1	79	5.05e-34	115
MS.gene50745.t1	MTR_1g087040	37.879	132	67	2	17	148	14	130	5.79e-24	91.3
MS.gene50745.t1	MTR_7g029400	48.485	99	47	2	1	98	57	152	3.02e-21	85.5
MS.gene50745.t1	MTR_8g015250	53.012	83	38	1	17	98	60	142	4.00e-21	85.1
MS.gene50745.t1	MTR_6g016375	48.750	80	41	0	19	98	79	158	2.68e-18	78.2
MS.gene50745.t1	MTR_4g097440	37.838	111	67	1	1	109	44	154	4.71e-18	76.6
MS.gene50745.t1	MTR_7g017880	48.750	80	41	0	19	98	76	155	1.22e-17	76.3
MS.gene50745.t1	MTR_5g015090	45.679	81	44	0	27	107	58	138	9.83e-16	70.5
MS.gene50745.t1	MTR_3g109310	36.111	108	65	1	6	109	98	205	1.17e-13	67.0
MS.gene50745.t1	MTR_7g109510	45.631	103	47	1	7	109	8	101	1.03e-11	59.3
MS.gene50745.t1	MTR_1g017570	45.455	66	36	0	29	94	59	124	7.32e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50745.t1	AT4G34590	61.429	140	46	4	24	156	21	159	2.65e-49	156
MS.gene50745.t1	AT1G75390	53.846	156	66	3	2	151	9	164	6.56e-47	150
MS.gene50745.t1	AT2G18160	50.345	145	56	4	27	156	28	171	9.59e-40	132
MS.gene50745.t1	AT1G75390	59.794	97	35	2	2	94	9	105	2.33e-32	112
MS.gene50745.t1	AT3G62420	44.615	130	67	1	25	154	20	144	5.99e-29	103
MS.gene50745.t1	AT5G15830	46.591	88	47	0	26	113	70	157	5.82e-19	79.3
MS.gene50745.t1	AT3G30530	53.425	73	34	0	26	98	77	149	9.58e-19	78.6
MS.gene50745.t1	AT1G13600	47.059	85	43	1	16	98	70	154	1.05e-18	79.0
MS.gene50745.t1	AT2G04038	48.810	84	41	1	17	98	59	142	1.43e-18	77.8
MS.gene50745.t1	AT5G38800	48.750	80	41	0	19	98	61	140	6.12e-17	73.6
MS.gene50745.t1	AT5G49450	38.053	113	50	3	1	110	1	96	5.21e-13	62.8
MS.gene50745.t1	AT4G37730	42.500	80	39	1	26	98	193	272	4.12e-12	62.8
MS.gene50745.t1	AT2G22850	39.286	84	51	0	26	109	125	208	3.06e-11	59.7
MS.gene50745.t1	AT2G22850	39.286	84	51	0	26	109	125	208	3.06e-11	59.7
MS.gene50745.t1	AT1G68880	43.284	67	38	0	28	94	45	111	5.32e-11	57.4

Find 28 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTATATGAACCAGCCTATTA+TGG	0.236275	4.3:-34294677	MS.gene50745:CDS
AACTCCATTGCTTGAATTCA+AGG	0.317165	4.3:+34294768	None:intergenic
TCATCAAGTGGAACATCTTC+AGG	0.318453	4.3:-34295093	MS.gene50745:CDS
AGAAGAATTACAGGCTATAA+TGG	0.323452	4.3:-34295040	MS.gene50745:CDS
AGGTTCAGAAGAAGAATTAC+AGG	0.357684	4.3:-34295049	MS.gene50745:CDS
ATATCTGCAGAAGCCATAAT+AGG	0.384326	4.3:+34294664	None:intergenic
AGATGGGAGAGTTGAGCAAC+AGG	0.420852	4.3:-34294819	MS.gene50745:CDS
TGGATCAGAGAAAGAGGAAG+AGG	0.433685	4.3:-34295020	MS.gene50745:CDS
GCGAATCGGCAAGGCGGTCT+AGG	0.434095	4.3:-34294984	MS.gene50745:CDS
AGCTGAGAATTCAGTGTTGA+GGG	0.459385	4.3:-34294845	MS.gene50745:CDS
TTCAGTGTTGAGGGCTCAGA+TGG	0.472933	4.3:-34294836	MS.gene50745:CDS
GAATCTTTGAATGAAATTGT+TGG	0.493743	4.3:-34294793	MS.gene50745:CDS
TAGACCGCCTTGCCGATTCG+CGG	0.495356	4.3:+34294986	None:intergenic
GATGATATCGAACCGCGAAT+CGG	0.496807	4.3:-34294998	MS.gene50745:CDS
CGGCAAGGCGGTCTAGGATG+AGG	0.519007	4.3:-34294978	MS.gene50745:CDS
TTGAAGAAGAAACCATTACT+TGG	0.526748	4.3:+34294718	None:intergenic
CTCATCATGGCTTCATCAAG+TGG	0.531339	4.3:-34295105	None:intergenic
CTATAATGGATCAGAGAAAG+AGG	0.546848	4.3:-34295026	MS.gene50745:CDS
CTGCAGAAGCCATAATAGGC+TGG	0.561236	4.3:+34294668	None:intergenic
GGGAGAGTTGAGCAACAGGT+TGG	0.564792	4.3:-34294815	MS.gene50745:CDS
GATGAGGAAACAGAAGCACT+TGG	0.601620	4.3:-34294962	MS.gene50745:CDS
AAGCTGAGAATTCAGTGTTG+AGG	0.603402	4.3:-34294846	MS.gene50745:CDS
TATCGAACCGCGAATCGGCA+AGG	0.622907	4.3:-34294993	MS.gene50745:CDS
TGTCACAAGTTGCTAAGCTG+AGG	0.639621	4.3:-34294930	MS.gene50745:CDS
TCATCTCTACTACAAAACTC+AGG	0.649209	4.3:-34295069	MS.gene50745:CDS
TCAGTGTTGAGGGCTCAGAT+GGG	0.657665	4.3:-34294835	MS.gene50745:CDS
GGTGCCTTGAATTCAAGCAA+TGG	0.668976	4.3:-34294772	MS.gene50745:CDS
CGAACCGCGAATCGGCAAGG+CGG	0.745432	4.3:-34294990	MS.gene50745:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GAATCTTTGAATGAAATTGT+TGG	-	chr4.3:34294957-34294976	MS.gene50745:CDS	25.0%
	AGAAGAATTACAGGCTATAA+TGG	-	chr4.3:34294710-34294729	MS.gene50745:CDS	30.0%
	TTATATGAACCAGCCTATTA+TGG	-	chr4.3:34295073-34295092	MS.gene50745:CDS	30.0%
	TTGAAGAAGAAACCATTACT+TGG	+	chr4.3:34295035-34295054	None:intergenic	30.0%
!	AACACTCAAATACTTTTGAG+TGG	+	chr4.3:34294883-34294902	None:intergenic	30.0%
!	AATTCAAGCAATGGAGTTTT+TGG	-	chr4.3:34294987-34295006	MS.gene50745:CDS	30.0%
	AACTCCATTGCTTGAATTCA+AGG	+	chr4.3:34294985-34295004	None:intergenic	35.0%
	AGGTTCAGAAGAAGAATTAC+AGG	-	chr4.3:34294701-34294720	MS.gene50745:CDS	35.0%
	ATATCTGCAGAAGCCATAAT+AGG	+	chr4.3:34295089-34295108	None:intergenic	35.0%
	TCATCTCTACTACAAAACTC+AGG	-	chr4.3:34294681-34294700	MS.gene50745:CDS	35.0%
!!	CTATAATGGATCAGAGAAAG+AGG	-	chr4.3:34294724-34294743	MS.gene50745:CDS	35.0%
	AAGCTGAGAATTCAGTGTTG+AGG	-	chr4.3:34294904-34294923	MS.gene50745:CDS	40.0%
	TCATCAAGTGGAACATCTTC+AGG	-	chr4.3:34294657-34294676	MS.gene50745:CDS	40.0%
!	AGCTGAGAATTCAGTGTTGA+GGG	-	chr4.3:34294905-34294924	MS.gene50745:CDS	40.0%
!	GCTTTTGTTGAGCCAAGTAA+TGG	-	chr4.3:34295020-34295039	MS.gene50745:CDS	40.0%
	GATGATATCGAACCGCGAAT+CGG	-	chr4.3:34294752-34294771	MS.gene50745:CDS	45.0%
	GGTGCCTTGAATTCAAGCAA+TGG	-	chr4.3:34294978-34294997	MS.gene50745:CDS	45.0%
	TGGATCAGAGAAAGAGGAAG+AGG	-	chr4.3:34294730-34294749	MS.gene50745:CDS	45.0%
	TGTCACAAGTTGCTAAGCTG+AGG	-	chr4.3:34294820-34294839	MS.gene50745:CDS	45.0%
!!	GATGAGGAAACAGAAGCACT+TGG	-	chr4.3:34294788-34294807	MS.gene50745:CDS	45.0%
	AGATGGGAGAGTTGAGCAAC+AGG	-	chr4.3:34294931-34294950	MS.gene50745:CDS	50.0%
	CTGCAGAAGCCATAATAGGC+TGG	+	chr4.3:34295085-34295104	None:intergenic	50.0%
!	TCAGTGTTGAGGGCTCAGAT+GGG	-	chr4.3:34294915-34294934	MS.gene50745:CDS	50.0%
!	TTCAGTGTTGAGGGCTCAGA+TGG	-	chr4.3:34294914-34294933	MS.gene50745:CDS	50.0%
	GGGAGAGTTGAGCAACAGGT+TGG	-	chr4.3:34294935-34294954	MS.gene50745:CDS	55.0%
	TATCGAACCGCGAATCGGCA+AGG	-	chr4.3:34294757-34294776	MS.gene50745:CDS	55.0%
!!	TAGACCGCCTTGCCGATTCG+CGG	+	chr4.3:34294767-34294786	None:intergenic	60.0%
	CGAACCGCGAATCGGCAAGG+CGG	-	chr4.3:34294760-34294779	MS.gene50745:CDS	65.0%
	GCGAATCGGCAAGGCGGTCT+AGG	-	chr4.3:34294766-34294785	MS.gene50745:CDS	65.0%
!	CGGCAAGGCGGTCTAGGATG+AGG	-	chr4.3:34294772-34294791	MS.gene50745:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	34294651	34295121	34294651	ID=MS.gene50745
chr4.3	mRNA	34294651	34295121	34294651	ID=MS.gene50745.t1;Parent=MS.gene50745
chr4.3	exon	34294651	34295121	34294651	ID=MS.gene50745.t1.exon1;Parent=MS.gene50745.t1
chr4.3	CDS	34294651	34295121	34294651	ID=cds.MS.gene50745.t1;Parent=MS.gene50745.t1

Gene Sequence

>MS.gene50745

ATGGCTTCATCAAGTGGAACATCTTCAGGTTCATCTCTACTACAAAACTCAGGTTCAGAAGAAGAATTACAGGCTATAATGGATCAGAGAAAGAGGAAGAGGATGATATCGAACCGCGAATCGGCAAGGCGGTCTAGGATGAGGAAACAGAAGCACTTGGATGATCTTGTGTCACAAGTTGCTAAGCTGAGGAAAGAGAATCAAGAAATACTCACAAGTGTTAACATCACCACTCAAAAGTATTTGAGTGTTGAAGCTGAGAATTCAGTGTTGAGGGCTCAGATGGGAGAGTTGAGCAACAGGTTGGAATCTTTGAATGAAATTGTTGGTGCCTTGAATTCAAGCAATGGAGTTTTTGGAGCATCAAATGCTTTTGTTGAGCCAAGTAATGGTTTCTTCTTCAACTCTTTGAATAATATGTCTTATATGAACCAGCCTATTATGGCTTCTGCAGATATACTGCAGTACTGA

Protein sequence

>MS.gene50745.t1

MASSSGTSSGSSLLQNSGSEEELQAIMDQRKRKRMISNRESARRSRMRKQKHLDDLVSQVAKLRKENQEILTSVNITTQKYLSVEAENSVLRAQMGELSNRLESLNEIVGALNSSNGVFGASNAFVEPSNGFFFNSLNNMSYMNQPIMASADILQY