AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068946


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068946.t1 MTR_8g046460 93.284 268 12 2 1 262 1 268 2.55e-178 491
MS.gene068946.t1 MTR_2g064370 70.896 268 71 4 1 262 1 267 1.80e-131 373
MS.gene068946.t1 MTR_8g028425 57.143 154 63 2 72 223 92 244 5.94e-65 203
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068946.t1 AT1G16320 69.231 221 67 1 43 262 53 273 2.78e-117 337
MS.gene068946.t1 AT1G79510 61.131 283 81 6 1 262 1 275 1.73e-116 335
MS.gene068946.t1 AT1G79510 61.131 283 81 6 1 262 1 275 1.73e-116 335
MS.gene068946.t1 AT2G46220 57.325 157 64 2 69 223 81 236 3.14e-66 206

Find 57 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAAACCGGTTTCGGTTT+TGG 0.220818 8.1:+58285238 MS.gene068946:CDS
GACCCTTTGAACACATTTAC+TGG 0.240823 8.1:+58284954 MS.gene068946:CDS
AGGTACTTCTCGGTATAAAT+TGG 0.261999 8.1:+58285151 MS.gene068946:CDS
AGAAGTACAAGTTGATCTTT+TGG 0.270946 8.1:+58284982 MS.gene068946:CDS
ACCTTAATTATGATATTTAT+AGG 0.278702 8.1:+58284504 MS.gene068946:CDS
ACCTATAAATATCATAATTA+AGG 0.297853 8.1:-58284505 None:intergenic
CCCCATGAAAATGTTGGATT+TGG 0.313331 8.1:-58285287 None:intergenic
TGGATTCTAGTTCATGTTCA+TGG 0.337564 8.1:+58285315 MS.gene068946:CDS
GAATAAAGAGTATTGAGAAA+TGG 0.346750 8.1:-58284312 None:intergenic
AATAAAGAGTATTGAGAAAT+GGG 0.349742 8.1:-58284311 None:intergenic
GTTTGGATGTATACAGAATA+TGG 0.351747 8.1:+58285045 MS.gene068946:CDS
AATGTTGGATTTGGACTTGC+AGG 0.366743 8.1:-58285278 None:intergenic
CAACCTGTGCAGTGAAAGTT+TGG 0.369856 8.1:-58284355 None:intergenic
TTGGTGTTTGAATCTTGATT+TGG 0.370847 8.1:-58284270 None:intergenic
TGTTTATCTTGAGTAGCTAA+TGG 0.394872 8.1:-58284392 None:intergenic
GAAGATGATGAAACCGGTTT+CGG 0.396965 8.1:+58285232 MS.gene068946:CDS
GTTGTTACCACCAGATGGTT+TGG 0.412534 8.1:+58285385 MS.gene068946:CDS
GTGAATTTCAAGGTACTTCT+CGG 0.412707 8.1:+58285141 MS.gene068946:CDS
GGAACTTGAGGGGTGTGCCT+AGG 0.417152 8.1:+58285099 MS.gene068946:CDS
TTTGTTTCGTGAGATTGGTT+TGG 0.421815 8.1:+58285028 MS.gene068946:CDS
GAACTTGAGGGGTGTGCCTA+GGG 0.426698 8.1:+58285100 MS.gene068946:CDS
ACCAAATCCAAAACCGAAAC+CGG 0.442141 8.1:-58285245 None:intergenic
TTTCACTGCACAGGTTGCTC+AGG 0.448262 8.1:+58284361 MS.gene068946:CDS
GGGGTGTGCCTAGGGTTCCA+TGG 0.454206 8.1:+58285108 MS.gene068946:CDS
GGGTGTGCCTAGGGTTCCAT+GGG 0.462556 8.1:+58285109 MS.gene068946:CDS
TTGAATCTTGATTTGGATTG+AGG 0.469278 8.1:-58284263 None:intergenic
CAGAGAATGTGATATTGATC+AGG 0.478427 8.1:+58285075 MS.gene068946:CDS
TACCAGTAAATGTGTTCAAA+GGG 0.481927 8.1:-58284956 None:intergenic
TCCACAGAAGATGATGAAAC+CGG 0.487224 8.1:+58285226 MS.gene068946:CDS
GAACATGTAGCCAAACCATC+TGG 0.493057 8.1:-58285395 None:intergenic
AGTCCAAACTTTCACTGCAC+AGG 0.494334 8.1:+58284352 MS.gene068946:CDS
ATACCAGTAAATGTGTTCAA+AGG 0.496720 8.1:-58284957 None:intergenic
GAAGCTAAAGGTGAATTTCA+AGG 0.517611 8.1:+58285131 MS.gene068946:CDS
ATCAATATCACATTCTCTGA+AGG 0.533171 8.1:-58285071 None:intergenic
AATTCACCTTTAGCTTCCCA+TGG 0.534577 8.1:-58285125 None:intergenic
TTAGCTTCCCATGGAACCCT+AGG 0.536034 8.1:-58285116 None:intergenic
TATTGATCAGGTGGAACTTG+AGG 0.540071 8.1:+58285087 MS.gene068946:CDS
AAAGGACCCCATGAAAATGT+TGG 0.541364 8.1:-58285293 None:intergenic
ATTGATCAGGTGGAACTTGA+GGG 0.551874 8.1:+58285088 MS.gene068946:CDS
ACCGGTTTCATCATCTTCTG+TGG 0.561359 8.1:-58285227 None:intergenic
GAAAGATTGAGATTGCAAAG+AGG 0.569214 8.1:-58284233 None:intergenic
AGGGTTCCATGGGAAGCTAA+AGG 0.578430 8.1:+58285119 MS.gene068946:CDS
AGGTCTTTAACAAAGATCAA+AGG 0.587544 8.1:-58284485 None:intergenic
AGACAGTTGTTACCACCAGA+TGG 0.589181 8.1:+58285380 MS.gene068946:CDS
TAAACAGAATCAGCATCAGA+AGG 0.609028 8.1:+58284409 MS.gene068946:CDS
AGAATGTGATATTGATCAGG+TGG 0.610163 8.1:+58285078 MS.gene068946:CDS
CTTCTCGGTATAAATTGGAT+CGG 0.610878 8.1:+58285156 MS.gene068946:CDS
ATGTTGGATTTGGACTTGCA+GGG 0.611890 8.1:-58285277 None:intergenic
GCAGTGAAAGTTTGGACTGA+AGG 0.616805 8.1:-58284347 None:intergenic
TCAAGCAGTTAAGCACACAT+TGG 0.624208 8.1:+58285349 MS.gene068946:CDS
CGGTATAAATTGGATCGGAA+CGG 0.634092 8.1:+58285161 MS.gene068946:CDS
GAACATGAACTAGAATCCAA+AGG 0.647700 8.1:-58285311 None:intergenic
TGCTGTTAGAACCCTTCGTG+AGG 0.651000 8.1:+58284457 MS.gene068946:CDS
TTGATCAGGTGGAACTTGAG+GGG 0.654298 8.1:+58285089 MS.gene068946:CDS
CAAAGGCATATCCTCACGAA+GGG 0.657035 8.1:-58284468 None:intergenic
TCAAAGGCATATCCTCACGA+AGG 0.664210 8.1:-58284469 None:intergenic
CATGTAGCCAAACCATCTGG+TGG 0.698747 8.1:-58285392 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACCTATAAATATCATAATTA+AGG - chr8.1:58284508-58284527 None:intergenic 15.0%
!! ACCTTAATTATGATATTTAT+AGG + chr8.1:58284504-58284523 MS.gene068946:CDS 15.0%
!! GATGATGTAATTAATATTTA+TGG + chr8.1:58284650-58284669 MS.gene068946:intron 15.0%
!! TTAATTCTAAGTAATATGTA+AGG + chr8.1:58284714-58284733 MS.gene068946:intron 15.0%
!!! AAAAATTGGATTTTTAATGT+TGG + chr8.1:58284611-58284630 MS.gene068946:intron 15.0%
!!! AAAATTGGATTTTTAATGTT+GGG + chr8.1:58284612-58284631 MS.gene068946:intron 15.0%
!!! TAATTTGAATTGTGTTATTT+AGG + chr8.1:58284584-58284603 MS.gene068946:intron 15.0%
!! GTTATTTAGGTGAAAAAAAT+TGG + chr8.1:58284597-58284616 MS.gene068946:intron 20.0%
!!! AATAAAGAGTATTGAGAAAT+GGG - chr8.1:58284314-58284333 None:intergenic 20.0%
!!! TGAGTTTGATAATTTTTTCA+GGG + chr8.1:58284914-58284933 MS.gene068946:intron 20.0%
!!! TTGAGTTTGATAATTTTTTC+AGG + chr8.1:58284913-58284932 MS.gene068946:intron 20.0%
!!! TTGCTTTTGATGTAAATATA+TGG + chr8.1:58284557-58284576 MS.gene068946:intron 20.0%
! AACTTTGAGTGGAAAAAAAT+TGG + chr8.1:58284853-58284872 MS.gene068946:intron 25.0%
! ACTTTGAGTGGAAAAAAATT+GGG + chr8.1:58284854-58284873 MS.gene068946:intron 25.0%
! ATTAATATTTATGGACCCTT+TGG + chr8.1:58284659-58284678 MS.gene068946:intron 25.0%
! CAAAAATAGTTAACTTTGAG+TGG + chr8.1:58284842-58284861 MS.gene068946:intron 25.0%
!! TATAGGTAATTTTCTCATCT+GGG + chr8.1:58284521-58284540 MS.gene068946:intron 25.0%
!! TGTTGGGTTTAGTAATTTTA+AGG + chr8.1:58284628-58284647 MS.gene068946:intron 25.0%
!! TTATAGGTAATTTTCTCATC+TGG + chr8.1:58284520-58284539 MS.gene068946:intron 25.0%
!!! GAATAAAGAGTATTGAGAAA+TGG - chr8.1:58284315-58284334 None:intergenic 25.0%
!!! TTATTTTGTGCAATAAGAGT+TGG + chr8.1:58284880-58284899 MS.gene068946:intron 25.0%
AACCATATATTCACATCCAA+AGG - chr8.1:58284678-58284697 None:intergenic 30.0%
ACCATATATTCACATCCAAA+GGG - chr8.1:58284677-58284696 None:intergenic 30.0%
AGGTCTTTAACAAAGATCAA+AGG - chr8.1:58284488-58284507 None:intergenic 30.0%
ATACCAGTAAATGTGTTCAA+AGG - chr8.1:58284960-58284979 None:intergenic 30.0%
ATCAATATCACATTCTCTGA+AGG - chr8.1:58285074-58285093 None:intergenic 30.0%
GTTTGGATGTATACAGAATA+TGG + chr8.1:58285045-58285064 MS.gene068946:CDS 30.0%
TACCAGTAAATGTGTTCAAA+GGG - chr8.1:58284959-58284978 None:intergenic 30.0%
TGTTTATCTTGAGTAGCTAA+TGG - chr8.1:58284395-58284414 None:intergenic 30.0%
! TCCAAATCCAACATTTTCAT+GGG + chr8.1:58285286-58285305 MS.gene068946:CDS 30.0%
! TTGGTGTTTGAATCTTGATT+TGG - chr8.1:58284273-58284292 None:intergenic 30.0%
!! AGAATTTTGTTTCGTGAGAT+TGG + chr8.1:58285023-58285042 MS.gene068946:CDS 30.0%
!! GATGAGTTTTATCTCAATCT+TGG + chr8.1:58284431-58284450 MS.gene068946:CDS 30.0%
!! TTGAATCTTGATTTGGATTG+AGG - chr8.1:58284266-58284285 None:intergenic 30.0%
!!! AGAAGTACAAGTTGATCTTT+TGG + chr8.1:58284982-58285001 MS.gene068946:CDS 30.0%
!!! CTATTTTTGTCCATACAAAG+TGG - chr8.1:58284831-58284850 None:intergenic 30.0%
!!! GAAGTACAAGTTGATCTTTT+GGG + chr8.1:58284983-58285002 MS.gene068946:CDS 30.0%
!!! GTTTTGAAGAAGAAAGTGTT+TGG - chr8.1:58284292-58284311 None:intergenic 30.0%
ACCCTTTGGATGTGAATATA+TGG + chr8.1:58284673-58284692 MS.gene068946:intron 35.0%
AGAATGTGATATTGATCAGG+TGG + chr8.1:58285078-58285097 MS.gene068946:CDS 35.0%
AGGTACTTCTCGGTATAAAT+TGG + chr8.1:58285151-58285170 MS.gene068946:CDS 35.0%
CAGAGAATGTGATATTGATC+AGG + chr8.1:58285075-58285094 MS.gene068946:CDS 35.0%
CTTCTCGGTATAAATTGGAT+CGG + chr8.1:58285156-58285175 MS.gene068946:CDS 35.0%
GAAAGATTGAGATTGCAAAG+AGG - chr8.1:58284236-58284255 None:intergenic 35.0%
GAACATGAACTAGAATCCAA+AGG - chr8.1:58285314-58285333 None:intergenic 35.0%
GAAGCTAAAGGTGAATTTCA+AGG + chr8.1:58285131-58285150 MS.gene068946:CDS 35.0%
TAAACAGAATCAGCATCAGA+AGG + chr8.1:58284409-58284428 MS.gene068946:CDS 35.0%
! CAGGGATGATGTAACATTTT+TGG + chr8.1:58284932-58284951 MS.gene068946:intron 35.0%
! CCAAATCCAACATTTTCATG+GGG + chr8.1:58285287-58285306 MS.gene068946:CDS 35.0%
! GTCCAAATCCAACATTTTCA+TGG + chr8.1:58285285-58285304 MS.gene068946:CDS 35.0%
! GTGAATTTCAAGGTACTTCT+CGG + chr8.1:58285141-58285160 MS.gene068946:CDS 35.0%
! TTTGTTTCGTGAGATTGGTT+TGG + chr8.1:58285028-58285047 MS.gene068946:CDS 35.0%
!! AAACTCATTGCCACTTTGTA+TGG + chr8.1:58284818-58284837 MS.gene068946:intron 35.0%
!! TGGATTCTAGTTCATGTTCA+TGG + chr8.1:58285315-58285334 MS.gene068946:CDS 35.0%
AAAGGACCCCATGAAAATGT+TGG - chr8.1:58285296-58285315 None:intergenic 40.0%
AATGTTGGATTTGGACTTGC+AGG - chr8.1:58285281-58285300 None:intergenic 40.0%
ACCAAATCCAAAACCGAAAC+CGG - chr8.1:58285248-58285267 None:intergenic 40.0%
AGTCGTTCGAACCTAAGTTA+AGG - chr8.1:58284771-58284790 None:intergenic 40.0%
ATGTTGGATTTGGACTTGCA+GGG - chr8.1:58285280-58285299 None:intergenic 40.0%
ATTGATCAGGTGGAACTTGA+GGG + chr8.1:58285088-58285107 MS.gene068946:CDS 40.0%
CCCCATGAAAATGTTGGATT+TGG - chr8.1:58285290-58285309 None:intergenic 40.0%
CGGTATAAATTGGATCGGAA+CGG + chr8.1:58285161-58285180 MS.gene068946:CDS 40.0%
GACCCTTTGAACACATTTAC+TGG + chr8.1:58284954-58284973 MS.gene068946:CDS 40.0%
TATTGATCAGGTGGAACTTG+AGG + chr8.1:58285087-58285106 MS.gene068946:CDS 40.0%
TCAAGCAGTTAAGCACACAT+TGG + chr8.1:58285349-58285368 MS.gene068946:CDS 40.0%
TCCACAGAAGATGATGAAAC+CGG + chr8.1:58285226-58285245 MS.gene068946:CDS 40.0%
! AACATTTTCATGGGGTCCTT+TGG + chr8.1:58285295-58285314 MS.gene068946:CDS 40.0%
! AATTCACCTTTAGCTTCCCA+TGG - chr8.1:58285128-58285147 None:intergenic 40.0%
! GAAGATGATGAAACCGGTTT+CGG + chr8.1:58285232-58285251 MS.gene068946:CDS 40.0%
!! AGTTGATCTTTTGGGCATTG+AGG + chr8.1:58284991-58285010 MS.gene068946:CDS 40.0%
!!! TTTTGGGCATTGAGGTTTCA+TGG + chr8.1:58284999-58285018 MS.gene068946:CDS 40.0%
AGACAGTTGTTACCACCAGA+TGG + chr8.1:58285380-58285399 MS.gene068946:CDS 45.0%
AGTCCAAACTTTCACTGCAC+AGG + chr8.1:58284352-58284371 MS.gene068946:CDS 45.0%
CAACCTGTGCAGTGAAAGTT+TGG - chr8.1:58284358-58284377 None:intergenic 45.0%
CTCAACTGGCAAATGTCGAT+CGG + chr8.1:58284742-58284761 MS.gene068946:intron 45.0%
GAACATGTAGCCAAACCATC+TGG - chr8.1:58285398-58285417 None:intergenic 45.0%
GCAGTGAAAGTTTGGACTGA+AGG - chr8.1:58284350-58284369 None:intergenic 45.0%
GTTGTTACCACCAGATGGTT+TGG + chr8.1:58285385-58285404 MS.gene068946:CDS 45.0%
TATGTAAGGCCTAGCTCAAC+TGG + chr8.1:58284728-58284747 MS.gene068946:intron 45.0%
TCGATCGGACACCTTAACTT+AGG + chr8.1:58284757-58284776 MS.gene068946:intron 45.0%
TTGATCAGGTGGAACTTGAG+GGG + chr8.1:58285089-58285108 MS.gene068946:CDS 45.0%
! ACCGGTTTCATCATCTTCTG+TGG - chr8.1:58285230-58285249 None:intergenic 45.0%
! CAAAGGCATATCCTCACGAA+GGG - chr8.1:58284471-58284490 None:intergenic 45.0%
! TCAAAGGCATATCCTCACGA+AGG - chr8.1:58284472-58284491 None:intergenic 45.0%
!! GATGAAACCGGTTTCGGTTT+TGG + chr8.1:58285238-58285257 MS.gene068946:CDS 45.0%
!!! ACCGGTTTCGGTTTTGGATT+TGG + chr8.1:58285244-58285263 MS.gene068946:CDS 45.0%
AGGGTTCCATGGGAAGCTAA+AGG + chr8.1:58285119-58285138 MS.gene068946:CDS 50.0%
CATGTAGCCAAACCATCTGG+TGG - chr8.1:58285395-58285414 None:intergenic 50.0%
CGACATTTGCCAGTTGAGCT+AGG - chr8.1:58284740-58284759 None:intergenic 50.0%
TAGGTTCGAACGACTCGTAG+TGG + chr8.1:58284776-58284795 MS.gene068946:intron 50.0%
TGCTGTTAGAACCCTTCGTG+AGG + chr8.1:58284457-58284476 MS.gene068946:CDS 50.0%
TTAGCTTCCCATGGAACCCT+AGG - chr8.1:58285119-58285138 None:intergenic 50.0%
TTTCACTGCACAGGTTGCTC+AGG + chr8.1:58284361-58284380 MS.gene068946:CDS 50.0%
! GAACTTGAGGGGTGTGCCTA+GGG + chr8.1:58285100-58285119 MS.gene068946:CDS 55.0%
! TCGAACGACTCGTAGTGGAG+TGG + chr8.1:58284781-58284800 MS.gene068946:intron 55.0%
GGGTGTGCCTAGGGTTCCAT+GGG + chr8.1:58285109-58285128 MS.gene068946:CDS 60.0%
! GGAACTTGAGGGGTGTGCCT+AGG + chr8.1:58285099-58285118 MS.gene068946:CDS 60.0%
GGGGTGTGCCTAGGGTTCCA+TGG + chr8.1:58285108-58285127 MS.gene068946:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 58284224 58285421 58284224 ID=MS.gene068946
chr8.1 mRNA 58284224 58285421 58284224 ID=MS.gene068946.t1;Parent=MS.gene068946
chr8.1 exon 58284224 58284525 58284224 ID=MS.gene068946.t1.exon1;Parent=MS.gene068946.t1
chr8.1 CDS 58284224 58284525 58284224 ID=cds.MS.gene068946.t1;Parent=MS.gene068946.t1
chr8.1 exon 58284935 58285421 58284935 ID=MS.gene068946.t1.exon2;Parent=MS.gene068946.t1
chr8.1 CDS 58284935 58285421 58284935 ID=cds.MS.gene068946.t1;Parent=MS.gene068946.t1
Gene Sequence

>MS.gene068946

ATGGCTTTTCCTCTTTGCAATCTCAATCTTTCTCTTTTACCTCAATCCAAATCAAGATTCAAACACCAAACACTTTCTTCTTCAAAACCCATTTCTCAATACTCTTTATTCTTCTCTTCTTCTCCTTCAGTCCAAACTTTCACTGCACAGGTTGCTCAGGTGAATTCACCATTAGCTACTCAAGATAAACAGAATCAGCATCAGAAGGATGAGTTTTATCTCAATCTTGGTGTTGCTGTTAGAACCCTTCGTGAGGATATGCCTTTGATCTTTGTTAAAGACCTTAATTATGATATTTATAGGTAATTTTCTCATCTGGGTATGTAAAGTTTCTTGCTTTTGATGTAAATATATGGTTTTTAATTTGAATTGTGTTATTTAGGTGAAAAAAATTGGATTTTTAATGTTGGGTTTAGTAATTTTAAGGATGATGTAATTAATATTTATGGACCCTTTGGATGTGAATATATGGTTTTTAATTCGAATTTAGTTAATTCTAAGTAATATGTAAGGCCTAGCTCAACTGGCAAATGTCGATCGGACACCTTAACTTAGGTTCGAACGACTCGTAGTGGAGTGGTGTATGTGAGTTTAAAACTCATTGCCACTTTGTATGGACAAAAATAGTTAACTTTGAGTGGAAAAAAATTGGGTTTTTATTTTGTGCAATAAGAGTTGGAATTTTAATGTTGAGTTTGATAATTTTTTCAGGGATGATGTAACATTTTTGGACCCTTTGAACACATTTACTGGTATTGAGAAGTACAAGTTGATCTTTTGGGCATTGAGGTTTCATGGTAGAATTTTGTTTCGTGAGATTGGTTTGGATGTATACAGAATATGGCAGCCTTCAGAGAATGTGATATTGATCAGGTGGAACTTGAGGGGTGTGCCTAGGGTTCCATGGGAAGCTAAAGGTGAATTTCAAGGTACTTCTCGGTATAAATTGGATCGGAACGGTAAGATTTATGAACACAAAGTTGATAATTTAGCATTCAACTTTCCACAGAAGATGATGAAACCGGTTTCGGTTTTGGATTTGGTTACTGCTTGCCCTGCAAGTCCAAATCCAACATTTTCATGGGGTCCTTTGGATTCTAGTTCATGTTCATGGATAGAATTATATCAAGCAGTTAAGCACACATTGGATGAAGAAAGACAGTTGTTACCACCAGATGGTTTGGCTACATGTTCATAG

Protein sequence

>MS.gene068946.t1

MAFPLCNLNLSLLPQSKSRFKHQTLSSSKPISQYSLFFSSSPSVQTFTAQVAQVNSPLATQDKQNQHQKDEFYLNLGVAVRTLREDMPLIFVKDLNYDIYRDDVTFLDPLNTFTGIEKYKLIFWALRFHGRILFREIGLDVYRIWQPSENVILIRWNLRGVPRVPWEAKGEFQGTSRYKLDRNGKIYEHKVDNLAFNFPQKMMKPVSVLDLVTACPASPNPTFSWGPLDSSSCSWIELYQAVKHTLDEERQLLPPDGLATCS