AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene41435


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41435.t1 MTR_1g100587 100.000 73 0 0 1 73 1 73 8.53e-34 112
MS.gene41435.t1 MTR_6g045257 87.671 73 9 0 4 76 3 75 5.66e-26 93.2
MS.gene41435.t1 MTR_8g098915 52.113 71 33 1 5 75 3 72 1.07e-17 72.8
MS.gene41435.t1 MTR_6g049260 45.333 75 40 1 5 79 3 76 1.69e-15 66.6
MS.gene41435.t1 MTR_6g051680 47.297 74 38 1 3 76 1 73 7.67e-15 65.1
MS.gene41435.t1 MTR_6g049320 48.571 70 35 1 4 73 32 100 3.12e-14 64.3
MS.gene41435.t1 MTR_1g099810 47.945 73 37 1 3 75 1 72 4.70e-14 63.5
MS.gene41435.t1 MTR_1g099800 47.945 73 37 1 3 75 1 72 6.64e-14 63.2
MS.gene41435.t1 MTR_6g049320 47.826 69 35 1 5 73 3 70 6.75e-14 62.8
MS.gene41435.t1 MTR_6g049280 47.143 70 36 1 4 73 32 100 7.91e-14 63.5
MS.gene41435.t1 MTR_6g049280 46.377 69 36 1 5 73 3 70 1.39e-13 62.0
MS.gene41435.t1 MTR_7g079110 49.275 69 34 1 3 71 1 68 1.64e-13 62.8
MS.gene41435.t1 MTR_3g434960 42.105 76 43 1 5 80 3 77 2.59e-13 60.8
MS.gene41435.t1 MTR_7g451210 43.243 74 41 1 5 78 3 75 2.89e-13 60.8
MS.gene41435.t1 MTR_3g434890 42.105 76 43 1 5 80 3 77 3.01e-13 60.8
MS.gene41435.t1 MTR_1g099640 52.055 73 34 1 4 76 2 73 4.55e-13 60.8
MS.gene41435.t1 MTR_6g051690 44.595 74 40 1 3 76 1 73 9.05e-13 60.1
MS.gene41435.t1 MTR_3g435000 39.706 68 41 0 13 80 14 81 1.45e-12 58.9
MS.gene41435.t1 MTR_8g098915 50.943 53 26 0 23 75 1 53 1.57e-12 58.9
MS.gene41435.t1 MTR_0795s0020 46.479 71 31 2 3 73 1 64 1.74e-12 59.3
MS.gene41435.t1 MTR_7g451200 40.789 76 44 1 5 80 3 77 2.10e-12 58.5
MS.gene41435.t1 MTR_1173s0010 40.789 76 44 1 5 80 3 77 2.17e-12 58.5
MS.gene41435.t1 MTR_1g099815 47.887 71 35 2 5 75 3 71 6.01e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41435.t1 AT5G23760 86.301 73 10 0 4 76 3 75 3.43e-24 88.6
MS.gene41435.t1 AT5G23760 84.375 64 10 0 13 76 1 64 2.87e-18 73.2
MS.gene41435.t1 AT5G52740 47.887 71 34 2 3 73 1 68 4.35e-11 55.5
MS.gene41435.t1 AT1G01490 44.118 102 43 3 3 91 1 101 6.37e-11 56.2
MS.gene41435.t1 AT1G01490 44.118 102 43 3 3 91 1 101 6.37e-11 56.2
MS.gene41435.t1 AT1G01490 45.000 100 41 3 5 91 30 128 6.75e-11 56.6

Find 11 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACAGAAGTTAACAGTTAT+TGG 0.231458 1.4:+78336299 MS.gene41435:CDS
ATAGCAAAAGGTGGTGTTAA+AGG 0.319190 1.4:+78336065 MS.gene41435:intron
GTTAACAGTTATTGGCGAAA+TGG 0.409498 1.4:+78336307 MS.gene41435:CDS
GAAGCAGTTGCTGATATCTA+TGG 0.414404 1.4:+78336129 MS.gene41435:CDS
GTGAAGAAGCTGAAGAAAGT+AGG 0.441545 1.4:+78336344 MS.gene41435:CDS
TGAAGAAGCTGAAGAAAGTA+GGG 0.447970 1.4:+78336345 MS.gene41435:CDS
CGAAAGTAAAGACGAAAAGA+AGG 0.460664 1.4:+78336427 MS.gene41435:CDS
AAGGTAGATATAATATCAGT+TGG 0.509973 1.4:+78336368 MS.gene41435:CDS
CTTCCCTGTGACAATGGCTC+AGG 0.517352 1.4:+78335686 None:intergenic
GAAGCTGAAGAAAGTAGGGA+AGG 0.555805 1.4:+78336349 MS.gene41435:CDS
ACTGGATCCATAGCAAAAGG+TGG 0.684112 1.4:+78336056 MS.gene41435:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTATTTTAAATTGTTTTTG+AGG + chr1.4:78335878-78335897 MS.gene41435:intron 15.0%
!!! CTATTTTAAATTGTTTTTGA+GGG + chr1.4:78335879-78335898 MS.gene41435:intron 15.0%
!!! AATTTAGTTTTCTGGTAAAT+TGG - chr1.4:78336185-78336204 None:intergenic 20.0%
! AAGGTAGATATAATATCAGT+TGG + chr1.4:78336368-78336387 MS.gene41435:CDS 25.0%
!!! AAATTGTTTTTGAGGGAATT+TGG + chr1.4:78335886-78335905 MS.gene41435:intron 25.0%
!!! TTCTTCTTTTTTCTCTTCTT+TGG - chr1.4:78336399-78336418 None:intergenic 25.0%
AAAATGAATATATGCGCACA+TGG - chr1.4:78335774-78335793 None:intergenic 30.0%
AAAGAAGGAAGAGAAAAAAG+AGG + chr1.4:78336442-78336461 MS.gene41435:CDS 30.0%
AAATTCATCTGTGTAACTGT+TGG + chr1.4:78336233-78336252 MS.gene41435:intron 30.0%
ACATAAGAGGATAGAAAAAG+TGG - chr1.4:78336014-78336033 None:intergenic 30.0%
ATCTATCTCTTGAGGTTATA+AGG + chr1.4:78335910-78335929 MS.gene41435:intron 30.0%
ATTCCATTAATGTGTTTCCA+GGG - chr1.4:78336212-78336231 None:intergenic 30.0%
CATATTGAGACAAACATAAG+AGG - chr1.4:78336027-78336046 None:intergenic 30.0%
GAACAGAAGTTAACAGTTAT+TGG + chr1.4:78336299-78336318 MS.gene41435:CDS 30.0%
TATTCCATTAATGTGTTTCC+AGG - chr1.4:78336213-78336232 None:intergenic 30.0%
! AATTTGGAATCTATCTCTTG+AGG + chr1.4:78335902-78335921 MS.gene41435:intron 30.0%
! ACTGTTGGTACTTTTATAAC+AGG + chr1.4:78336248-78336267 MS.gene41435:intron 30.0%
AGAAAACTAAATTTCTGCCC+TGG + chr1.4:78336192-78336211 MS.gene41435:intron 35.0%
AGGTATGGATTCTCTCATTT+GGG + chr1.4:78335706-78335725 MS.gene41435:intron 35.0%
CGAAAGTAAAGACGAAAAGA+AGG + chr1.4:78336427-78336446 MS.gene41435:CDS 35.0%
CTATAACCAAACAGTACATG+AGG - chr1.4:78335958-78335977 None:intergenic 35.0%
GAAAAAAGAGGAGAAACAAG+AGG + chr1.4:78336454-78336473 MS.gene41435:CDS 35.0%
GTTAACAGTTATTGGCGAAA+TGG + chr1.4:78336307-78336326 MS.gene41435:CDS 35.0%
TGAAGAAGCTGAAGAAAGTA+GGG + chr1.4:78336345-78336364 MS.gene41435:CDS 35.0%
! ATAGCAAAAGGTGGTGTTAA+AGG + chr1.4:78336065-78336084 MS.gene41435:intron 35.0%
! TTTAACACCACCTTTTGCTA+TGG - chr1.4:78336066-78336085 None:intergenic 35.0%
!!! AGGGCAGAAATTTAGTTTTC+TGG - chr1.4:78336193-78336212 None:intergenic 35.0%
!!! TCTTTTTTCTCTTCTTTGGC+AGG - chr1.4:78336395-78336414 None:intergenic 35.0%
CAGGTATGGATTCTCTCATT+TGG + chr1.4:78335705-78335724 MS.gene41435:intron 40.0%
GAAGCAGTTGCTGATATCTA+TGG + chr1.4:78336129-78336148 MS.gene41435:CDS 40.0%
GTGAAGAAGCTGAAGAAAGT+AGG + chr1.4:78336344-78336363 MS.gene41435:CDS 40.0%
TGAATATATGCGCACATGGA+TGG - chr1.4:78335770-78335789 None:intergenic 40.0%
!! TTTTGCTATGGATCCAGTGA+GGG - chr1.4:78336054-78336073 None:intergenic 40.0%
ACTGGATCCATAGCAAAAGG+TGG + chr1.4:78336056-78336075 MS.gene41435:intron 45.0%
CTCAATATGATGTCCCTCAC+TGG + chr1.4:78336038-78336057 MS.gene41435:intron 45.0%
CTCACTGGATCCATAGCAAA+AGG + chr1.4:78336053-78336072 MS.gene41435:intron 45.0%
CTGCCCTGGAAACACATTAA+TGG + chr1.4:78336206-78336225 MS.gene41435:intron 45.0%
GAAGCTGAAGAAAGTAGGGA+AGG + chr1.4:78336349-78336368 MS.gene41435:CDS 45.0%
!! CTTTTGCTATGGATCCAGTG+AGG - chr1.4:78336055-78336074 None:intergenic 45.0%
CTGACGCCTCATGTACTGTT+TGG + chr1.4:78335949-78335968 MS.gene41435:intron 50.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 78335699 78336487 78335699 ID=MS.gene41435
chr1.4 mRNA 78335699 78336487 78335699 ID=MS.gene41435.t1;Parent=MS.gene41435
chr1.4 exon 78335699 78335707 78335699 ID=MS.gene41435.t1.exon1;Parent=MS.gene41435.t1
chr1.4 CDS 78335699 78335707 78335699 ID=cds.MS.gene41435.t1;Parent=MS.gene41435.t1
chr1.4 exon 78336069 78336150 78336069 ID=MS.gene41435.t1.exon2;Parent=MS.gene41435.t1
chr1.4 CDS 78336069 78336150 78336069 ID=cds.MS.gene41435.t1;Parent=MS.gene41435.t1
chr1.4 exon 78336270 78336487 78336270 ID=MS.gene41435.t1.exon3;Parent=MS.gene41435.t1
chr1.4 CDS 78336270 78336487 78336270 ID=cds.MS.gene41435.t1;Parent=MS.gene41435.t1
Gene Sequence

>MS.gene41435

ATGGCTCAGGTATGGATTCTCTCATTTGGGTTTCTACAGAACAATTTTTTCTTGTTACTGTTAATACTCCATCCATGTGCGCATATATTCATTTTACAAACTCTAGCTGAATTATTATTAATTAAAGTGAAAAAAAATAAGAAATAGATAAATCATTATTAATTAAAGTGACACTAACGACTATTTTAAATTGTTTTTGAGGGAATTTGGAATCTATCTCTTGAGGTTATAAGGTCAAATTCTAGCTGAACTGACGCCTCATGTACTGTTTGGTTATAGTTAAATGAAAGATTCGATTTAGAATTTTGTTTACCACTTTTTCTATCCTCTTATGTTTGTCTCAATATGATGTCCCTCACTGGATCCATAGCAAAAGGTGGTGTTAAAGGTTCTCACTATGACAGATGACAAAACAAAGAAGAAAGCAATAGAAGCAGTTGCTGATATCTATGGTGAGAATTTATGATTTCATTTTCATTTGCTCCAATTTACCAGAAAACTAAATTTCTGCCCTGGAAACACATTAATGGAATAAAATTCATCTGTGTAACTGTTGGTACTTTTATAACAGGAGTTGATTCAATCGCTACAGATGTGAACGAACAGAAGTTAACAGTTATTGGCGAAATGGATACAGTTGCAGTAGTGAAGAAGCTGAAGAAAGTAGGGAAGGTAGATATAATATCAGTTGGACCTGCCAAAGAAGAGAAAAAAGAAGAAAAGAAAGACGAAAGTAAAGACGAAAAGAAGGAAGAGAAAAAAGAGGAGAAACAAGAGGAGAAAAAATAA

Protein sequence

>MS.gene41435.t1

MAQQKVVLKVLTMTDDKTKKKAIEAVADIYGVDSIATDVNEQKLTVIGEMDTVAVVKKLKKVGKVDIISVGPAKEEKKEEKKDESKDEKKEEKKEEKQEEKK