AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34496


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34496.t1 MTR_1g100587 100.000 73 0 0 1 73 1 73 8.53e-34 112
MS.gene34496.t1 MTR_6g045257 87.671 73 9 0 4 76 3 75 5.66e-26 93.2
MS.gene34496.t1 MTR_8g098915 52.113 71 33 1 5 75 3 72 1.07e-17 72.8
MS.gene34496.t1 MTR_6g049260 45.333 75 40 1 5 79 3 76 1.69e-15 66.6
MS.gene34496.t1 MTR_6g051680 47.297 74 38 1 3 76 1 73 7.67e-15 65.1
MS.gene34496.t1 MTR_6g049320 48.571 70 35 1 4 73 32 100 3.12e-14 64.3
MS.gene34496.t1 MTR_1g099810 47.945 73 37 1 3 75 1 72 4.70e-14 63.5
MS.gene34496.t1 MTR_1g099800 47.945 73 37 1 3 75 1 72 6.64e-14 63.2
MS.gene34496.t1 MTR_6g049320 47.826 69 35 1 5 73 3 70 6.75e-14 62.8
MS.gene34496.t1 MTR_6g049280 47.143 70 36 1 4 73 32 100 7.91e-14 63.5
MS.gene34496.t1 MTR_6g049280 46.377 69 36 1 5 73 3 70 1.39e-13 62.0
MS.gene34496.t1 MTR_7g079110 49.275 69 34 1 3 71 1 68 1.64e-13 62.8
MS.gene34496.t1 MTR_3g434960 42.105 76 43 1 5 80 3 77 2.59e-13 60.8
MS.gene34496.t1 MTR_7g451210 43.243 74 41 1 5 78 3 75 2.89e-13 60.8
MS.gene34496.t1 MTR_3g434890 42.105 76 43 1 5 80 3 77 3.01e-13 60.8
MS.gene34496.t1 MTR_1g099640 52.055 73 34 1 4 76 2 73 4.55e-13 60.8
MS.gene34496.t1 MTR_6g051690 44.595 74 40 1 3 76 1 73 9.05e-13 60.1
MS.gene34496.t1 MTR_3g435000 39.706 68 41 0 13 80 14 81 1.45e-12 58.9
MS.gene34496.t1 MTR_8g098915 50.943 53 26 0 23 75 1 53 1.57e-12 58.9
MS.gene34496.t1 MTR_0795s0020 46.479 71 31 2 3 73 1 64 1.74e-12 59.3
MS.gene34496.t1 MTR_7g451200 40.789 76 44 1 5 80 3 77 2.10e-12 58.5
MS.gene34496.t1 MTR_1173s0010 40.789 76 44 1 5 80 3 77 2.17e-12 58.5
MS.gene34496.t1 MTR_1g099815 47.887 71 35 2 5 75 3 71 6.01e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34496.t1 AT5G23760 86.301 73 10 0 4 76 3 75 3.43e-24 88.6
MS.gene34496.t1 AT5G23760 84.375 64 10 0 13 76 1 64 2.87e-18 73.2
MS.gene34496.t1 AT5G52740 47.887 71 34 2 3 73 1 68 4.35e-11 55.5
MS.gene34496.t1 AT1G01490 44.118 102 43 3 3 91 1 101 6.37e-11 56.2
MS.gene34496.t1 AT1G01490 44.118 102 43 3 3 91 1 101 6.37e-11 56.2
MS.gene34496.t1 AT1G01490 45.000 100 41 3 5 91 30 128 6.75e-11 56.6

Find 11 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACAGAAGTTAACAGTTAT+TGG 0.231458 1.2:+75356703 MS.gene34496:CDS
ATAGCAAAAGGTGGTGTTAA+AGG 0.319190 1.2:+75356475 MS.gene34496:intron
GTTAACAGTTATTGGCGAAA+TGG 0.409498 1.2:+75356711 MS.gene34496:CDS
GAAGCAGTTGCTGATATCTA+TGG 0.414404 1.2:+75356539 MS.gene34496:CDS
GTGAAGAAGCTGAAGAAAGT+AGG 0.441545 1.2:+75356748 MS.gene34496:CDS
TGAAGAAGCTGAAGAAAGTA+GGG 0.447970 1.2:+75356749 MS.gene34496:CDS
CGAAAGTAAAGACGAAAAGA+AGG 0.460664 1.2:+75356831 MS.gene34496:CDS
AAGGTAGATATAATATCAGT+TGG 0.509973 1.2:+75356772 MS.gene34496:CDS
CTTCCCTGTGACAATGGCTC+AGG 0.517352 1.2:+75356096 None:intergenic
GAAGCTGAAGAAAGTAGGGA+AGG 0.555805 1.2:+75356753 MS.gene34496:CDS
ACTGGATCCATAGCAAAAGG+TGG 0.684112 1.2:+75356466 MS.gene34496:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTATTTTAAATTGTTTTTG+AGG + chr1.2:75356288-75356307 MS.gene34496:intron 15.0%
!!! CTATTTTAAATTGTTTTTGA+GGG + chr1.2:75356289-75356308 MS.gene34496:intron 15.0%
!!! AATTTAGTTTTCTGGTAAAT+TGG - chr1.2:75356589-75356608 None:intergenic 20.0%
! AAGGTAGATATAATATCAGT+TGG + chr1.2:75356772-75356791 MS.gene34496:CDS 25.0%
!!! AAATTGTTTTTGAGGGAATT+TGG + chr1.2:75356296-75356315 MS.gene34496:intron 25.0%
!!! TTCTTCTTTTTTCTCTTCTT+TGG - chr1.2:75356803-75356822 None:intergenic 25.0%
AAAATGAATATATGCGCACA+TGG - chr1.2:75356184-75356203 None:intergenic 30.0%
AAAGAAGGAAGAGAAAAAAG+AGG + chr1.2:75356846-75356865 MS.gene34496:CDS 30.0%
AAATTCATCTGTGTAACTGT+TGG + chr1.2:75356637-75356656 MS.gene34496:intron 30.0%
ACATAAGAGGATAGAAAAAG+TGG - chr1.2:75356424-75356443 None:intergenic 30.0%
ATCTATCTCTTGAGGTTATA+AGG + chr1.2:75356320-75356339 MS.gene34496:intron 30.0%
ATTCCATTAATGTGTTTCCA+GGG - chr1.2:75356616-75356635 None:intergenic 30.0%
CATATTGAGACAAACATAAG+AGG - chr1.2:75356437-75356456 None:intergenic 30.0%
GAACAGAAGTTAACAGTTAT+TGG + chr1.2:75356703-75356722 MS.gene34496:CDS 30.0%
TATTCCATTAATGTGTTTCC+AGG - chr1.2:75356617-75356636 None:intergenic 30.0%
! AATTTGGAATCTATCTCTTG+AGG + chr1.2:75356312-75356331 MS.gene34496:intron 30.0%
ACTGTTGGTACTCTTATAAC+AGG + chr1.2:75356652-75356671 MS.gene34496:intron 35.0%
AGAAAACTAAATTTCTGCCC+TGG + chr1.2:75356596-75356615 MS.gene34496:intron 35.0%
AGGTATGGATTCTCTCATTT+GGG + chr1.2:75356116-75356135 MS.gene34496:intron 35.0%
CGAAAGTAAAGACGAAAAGA+AGG + chr1.2:75356831-75356850 MS.gene34496:CDS 35.0%
GAAAAAAGAGGAGAAACAAG+AGG + chr1.2:75356858-75356877 MS.gene34496:CDS 35.0%
GTTAACAGTTATTGGCGAAA+TGG + chr1.2:75356711-75356730 MS.gene34496:CDS 35.0%
TGAAGAAGCTGAAGAAAGTA+GGG + chr1.2:75356749-75356768 MS.gene34496:CDS 35.0%
! ATAGCAAAAGGTGGTGTTAA+AGG + chr1.2:75356475-75356494 MS.gene34496:intron 35.0%
! TTTAACACCACCTTTTGCTA+TGG - chr1.2:75356476-75356495 None:intergenic 35.0%
!!! AGGGCAGAAATTTAGTTTTC+TGG - chr1.2:75356597-75356616 None:intergenic 35.0%
!!! TCTTTTTTCTCTTCTTTGGC+AGG - chr1.2:75356799-75356818 None:intergenic 35.0%
CAGGTATGGATTCTCTCATT+TGG + chr1.2:75356115-75356134 MS.gene34496:intron 40.0%
CTATAACCAAACAGTACACG+AGG - chr1.2:75356368-75356387 None:intergenic 40.0%
GAAGCAGTTGCTGATATCTA+TGG + chr1.2:75356539-75356558 MS.gene34496:CDS 40.0%
GTGAAGAAGCTGAAGAAAGT+AGG + chr1.2:75356748-75356767 MS.gene34496:CDS 40.0%
TGAATATATGCGCACATGGA+TGG - chr1.2:75356180-75356199 None:intergenic 40.0%
!! TTTTGCTATGGATCCAGTGA+GGG - chr1.2:75356464-75356483 None:intergenic 40.0%
ACTGGATCCATAGCAAAAGG+TGG + chr1.2:75356466-75356485 MS.gene34496:intron 45.0%
CTCAATATGATGTCCCTCAC+TGG + chr1.2:75356448-75356467 MS.gene34496:intron 45.0%
CTCACTGGATCCATAGCAAA+AGG + chr1.2:75356463-75356482 MS.gene34496:intron 45.0%
CTGCCCTGGAAACACATTAA+TGG + chr1.2:75356610-75356629 MS.gene34496:intron 45.0%
GAAGCTGAAGAAAGTAGGGA+AGG + chr1.2:75356753-75356772 MS.gene34496:CDS 45.0%
!! CTTTTGCTATGGATCCAGTG+AGG - chr1.2:75356465-75356484 None:intergenic 45.0%
CTGACGCCTCGTGTACTGTT+TGG + chr1.2:75356359-75356378 MS.gene34496:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 75356109 75356891 75356109 ID=MS.gene34496
chr1.2 mRNA 75356109 75356891 75356109 ID=MS.gene34496.t1;Parent=MS.gene34496
chr1.2 exon 75356109 75356117 75356109 ID=MS.gene34496.t1.exon1;Parent=MS.gene34496.t1
chr1.2 CDS 75356109 75356117 75356109 ID=cds.MS.gene34496.t1;Parent=MS.gene34496.t1
chr1.2 exon 75356479 75356560 75356479 ID=MS.gene34496.t1.exon2;Parent=MS.gene34496.t1
chr1.2 CDS 75356479 75356560 75356479 ID=cds.MS.gene34496.t1;Parent=MS.gene34496.t1
chr1.2 exon 75356674 75356891 75356674 ID=MS.gene34496.t1.exon3;Parent=MS.gene34496.t1
chr1.2 CDS 75356674 75356891 75356674 ID=cds.MS.gene34496.t1;Parent=MS.gene34496.t1
Gene Sequence

>MS.gene34496

ATGGCTCAGGTATGGATTCTCTCATTTGGGTTTCTACAGAACAATTTTTTCTTGTTACTGTTAATACTCCATCCATGTGCGCATATATTCATTTTACAAACTCTAGCTGAATTATTATTAATTAAAGTGAAAAAAAATAAGAAATAGATAAATCATTATTAATTAAAGTGACACTAACGACTATTTTAAATTGTTTTTGAGGGAATTTGGAATCTATCTCTTGAGGTTATAAGGTCAAATTCTAGCTGAACTGACGCCTCGTGTACTGTTTGGTTATAGTTAAATGAAAGATTCGATTTAGAATTTTGTTTACCACTTTTTCTATCCTCTTATGTTTGTCTCAATATGATGTCCCTCACTGGATCCATAGCAAAAGGTGGTGTTAAAGGTTCTCACTATGACAGATGACAAAACAAAGAAGAAAGCAATAGAAGCAGTTGCTGATATCTATGGTGAGAATTTATGATTTCATTTGCTCCAATTTACCAGAAAACTAAATTTCTGCCCTGGAAACACATTAATGGAATAAAATTCATCTGTGTAACTGTTGGTACTCTTATAACAGGAGTTGATTCAATCGCTACAGATGTGAACGAACAGAAGTTAACAGTTATTGGCGAAATGGATACAGTTGCAGTAGTGAAGAAGCTGAAGAAAGTAGGGAAGGTAGATATAATATCAGTTGGACCTGCCAAAGAAGAGAAAAAAGAAGAAAAGAAAGACGAAAGTAAAGACGAAAAGAAGGAAGAGAAAAAAGAGGAGAAACAAGAGGAGAAAAAATAA

Protein sequence

>MS.gene34496.t1

MAQQKVVLKVLTMTDDKTKKKAIEAVADIYGVDSIATDVNEQKLTVIGEMDTVAVVKKLKKVGKVDIISVGPAKEEKKEEKKDESKDEKKEEKKEEKQEEKK