AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071722


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071722.t1 MTR_4g132350 99.338 151 1 0 1 151 72 222 1.16e-110 315
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071722.t1 AT1G62250 69.388 147 45 0 5 151 76 222 1.75e-73 219
MS.gene071722.t1 AT1G62250 68.874 151 46 1 2 151 72 222 1.90e-73 221
MS.gene071722.t1 AT1G62250 68.874 151 46 1 2 151 72 222 1.90e-73 221

Find 33 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGCACTGATGAGGGATTA+AGG 0.315940 8.4:+1701439 MS.gene071722:CDS
TTTCAGGAGTTCATCACTGC+TGG 0.339359 8.4:+1701396 MS.gene071722:intron
TCACCTTGTTCAGGAGATAT+AGG 0.401117 8.4:+1700654 MS.gene071722:CDS
ACATAGCCATCACCTTGTTC+AGG 0.430952 8.4:+1700645 MS.gene071722:CDS
TTCAGGAGTTCATCACTGCT+GGG 0.433998 8.4:+1701397 MS.gene071722:intron
CCCCACCGACTATTGTTAGA+TGG 0.484658 8.4:+1701726 MS.gene071722:CDS
GGGGTGAATGCATATGAACT+TGG 0.519443 8.4:+1701417 MS.gene071722:CDS
ATCTAAAATCATATCTAATG+AGG 0.520867 8.4:+1701539 MS.gene071722:CDS
AATTTCACTGATCGTGGTGG+AGG 0.547938 8.4:+1700677 MS.gene071722:CDS
ATTGAAGCAATGCAAAGTTA+CGG 0.554118 8.4:+1701498 MS.gene071722:CDS
GAAAGAGCTTACTGACATGA+AGG 0.564447 8.4:+1701461 MS.gene071722:CDS
CTTACTGACATGAAGGACTC+TGG 0.574764 8.4:+1701468 MS.gene071722:CDS
ACCATCTAACAATAGTCGGT+GGG 0.578238 8.4:-1701727 None:intergenic
CCATCTAACAATAGTCGGTG+GGG 0.583403 8.4:-1701726 None:intergenic
ATATCTCCTGAACAAGGTGA+TGG 0.598635 8.4:-1700651 None:intergenic
GACCATCTAACAATAGTCGG+TGG 0.598967 8.4:-1701728 None:intergenic
TGAACTTGGGTGCACTGATG+AGG 0.602547 8.4:+1701431 MS.gene071722:CDS
GATGACCATCTAACAATAGT+CGG 0.613459 8.4:-1701731 None:intergenic
TATAGGAATTTCACTGATCG+TGG 0.622160 8.4:+1700671 MS.gene071722:CDS
AAGCAATGCAAAGTTACGGT+GGG 0.622956 8.4:+1701502 MS.gene071722:CDS
GAAGTGAGACTTCAGTGGAA+AGG 0.627135 8.4:+1701773 MS.gene071722:CDS
GAACTTGGGTGCACTGATGA+GGG 0.632248 8.4:+1701432 MS.gene071722:CDS
GGGTGAATGCATATGAACTT+GGG 0.632813 8.4:+1701418 MS.gene071722:CDS
CATCTAACAATAGTCGGTGG+GGG 0.633024 8.4:-1701725 None:intergenic
TCAGGAGTTCATCACTGCTG+GGG 0.635874 8.4:+1701398 MS.gene071722:intron
ATTCCTATATCTCCTGAACA+AGG 0.635905 8.4:-1700657 None:intergenic
GAAGCAATGCAAAGTTACGG+TGG 0.636433 8.4:+1701501 MS.gene071722:CDS
AGGAATTTCACTGATCGTGG+TGG 0.644671 8.4:+1700674 MS.gene071722:CDS
TGGCTATGTTGCGTGAAGAA+CGG 0.675675 8.4:-1700631 None:intergenic
AGTCTCACTTCATCTGACAC+AGG 0.679981 8.4:-1701761 None:intergenic
CAGATGAAGTGAGACTTCAG+TGG 0.693954 8.4:+1701768 MS.gene071722:CDS
AGATTGATGAGTGCATTCTG+TGG 0.715212 8.4:+1701669 MS.gene071722:intron
ATGATATAATTGCAGCAGTG+TGG 0.719749 8.4:+1700575 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAGGAATGCATAAAAAT+GGG - chr8.4:1700889-1700908 None:intergenic 20.0%
!!! ATCTAAAATCATATCTAATG+AGG + chr8.4:1701539-1701558 MS.gene071722:CDS 20.0%
! AAACTCTATGTTTGAATTTG+TGG + chr8.4:1701001-1701020 MS.gene071722:intron 25.0%
! ATAAGGAATGCATAAAAATG+GGG - chr8.4:1700888-1700907 None:intergenic 25.0%
! ATGTTGAGTTATACAAGAAA+GGG - chr8.4:1701291-1701310 None:intergenic 25.0%
! CTAATTAGAGAGTTCAAATT+CGG - chr8.4:1700961-1700980 None:intergenic 25.0%
! CTATAAGGAATGCATAAAAA+TGG - chr8.4:1700890-1700909 None:intergenic 25.0%
! CTGGTTTAATTAAGTGTAAT+AGG + chr8.4:1700917-1700936 MS.gene071722:intron 25.0%
!! AGCAAATGCATATTTTATGA+AGG + chr8.4:1701811-1701830 MS.gene071722:CDS 25.0%
!! ATTTTCAATTATGATGCTAG+AGG - chr8.4:1700728-1700747 None:intergenic 25.0%
!! GATTTACGTTTTTCATTTTC+AGG + chr8.4:1701380-1701399 MS.gene071722:intron 25.0%
!! GCAAATGCATATTTTATGAA+GGG + chr8.4:1701812-1701831 MS.gene071722:CDS 25.0%
AAACGTAAATCACCTCTTAT+TGG - chr8.4:1701371-1701390 None:intergenic 30.0%
ACCTCTTATTGGAATATAGA+GGG - chr8.4:1701360-1701379 None:intergenic 30.0%
ACGTTGTACGTTGTTATAAT+AGG + chr8.4:1700822-1700841 MS.gene071722:intron 30.0%
AGATATCGAGTTACCTTATT+TGG + chr8.4:1701627-1701646 MS.gene071722:intron 30.0%
ATTGAAGCAATGCAAAGTTA+CGG + chr8.4:1701498-1701517 MS.gene071722:CDS 30.0%
CACAAAAAACATGCCAAATA+AGG - chr8.4:1701643-1701662 None:intergenic 30.0%
CATGTTGAGTTATACAAGAA+AGG - chr8.4:1701292-1701311 None:intergenic 30.0%
GATCTTGTTGAATCTTCAAA+TGG + chr8.4:1701052-1701071 MS.gene071722:intron 30.0%
TAAACCAGCTCAAAACTATA+AGG - chr8.4:1700905-1700924 None:intergenic 30.0%
! GAACTCTCTAATTAGTTCTT+TGG + chr8.4:1700966-1700985 MS.gene071722:intron 30.0%
! GTTTGAATTTGTGGTGATTT+TGG + chr8.4:1701010-1701029 MS.gene071722:intron 30.0%
! TGCATATTTTATGAAGGGAA+TGG + chr8.4:1701817-1701836 MS.gene071722:CDS 30.0%
ATTCCTATATCTCCTGAACA+AGG - chr8.4:1700660-1700679 None:intergenic 35.0%
CAATATGTATGCCACATTCA+CGG + chr8.4:1701099-1701118 MS.gene071722:intron 35.0%
CACCTCTTATTGGAATATAG+AGG - chr8.4:1701361-1701380 None:intergenic 35.0%
GATGACCATCTAACAATAGT+CGG - chr8.4:1701734-1701753 None:intergenic 35.0%
TAGTTCTTTGGCAAAGCAAT+AGG + chr8.4:1700978-1700997 MS.gene071722:intron 35.0%
TATAGGAATTTCACTGATCG+TGG + chr8.4:1700671-1700690 MS.gene071722:CDS 35.0%
TCCCTCTATATTCCAATAAG+AGG + chr8.4:1701356-1701375 MS.gene071722:intron 35.0%
! AACTTTTGCGAAAATCACGT+TGG + chr8.4:1701076-1701095 MS.gene071722:intron 35.0%
!!! CATTCCTTATAGTTTTGAGC+TGG + chr8.4:1700898-1700917 MS.gene071722:intron 35.0%
AAGCAATGCAAAGTTACGGT+GGG + chr8.4:1701502-1701521 MS.gene071722:CDS 40.0%
AATTATGATGCTAGAGGCAG+TGG - chr8.4:1700722-1700741 None:intergenic 40.0%
ACCATCTAACAATAGTCGGT+GGG - chr8.4:1701730-1701749 None:intergenic 40.0%
AGATTGATGAGTGCATTCTG+TGG + chr8.4:1701669-1701688 MS.gene071722:intron 40.0%
ATATCTCCTGAACAAGGTGA+TGG - chr8.4:1700654-1700673 None:intergenic 40.0%
CGTACAACGTGTATATCAAC+TGG - chr8.4:1700812-1700831 None:intergenic 40.0%
GAAAGAGCTTACTGACATGA+AGG + chr8.4:1701461-1701480 MS.gene071722:CDS 40.0%
GGAAAGAACCAACTAGCAAA+AGG - chr8.4:1701339-1701358 None:intergenic 40.0%
GGGTGAATGCATATGAACTT+GGG + chr8.4:1701418-1701437 MS.gene071722:CDS 40.0%
GTTTACATTGACCGTGAATG+TGG - chr8.4:1701113-1701132 None:intergenic 40.0%
TCACCTTGTTCAGGAGATAT+AGG + chr8.4:1700654-1700673 MS.gene071722:CDS 40.0%
! CCAACTAGCAAAAGGTGTAA+AGG - chr8.4:1701331-1701350 None:intergenic 40.0%
! CCTTTACACCTTTTGCTAGT+TGG + chr8.4:1701328-1701347 MS.gene071722:intron 40.0%
AATTTCACTGATCGTGGTGG+AGG + chr8.4:1700677-1700696 MS.gene071722:CDS 45.0%
ACATAGCCATCACCTTGTTC+AGG + chr8.4:1700645-1700664 MS.gene071722:CDS 45.0%
AGGAATTTCACTGATCGTGG+TGG + chr8.4:1700674-1700693 MS.gene071722:CDS 45.0%
AGTCTCACTTCATCTGACAC+AGG - chr8.4:1701764-1701783 None:intergenic 45.0%
CAACAAGATCACAGTGTCGT+TGG - chr8.4:1701042-1701061 None:intergenic 45.0%
CAGATGAAGTGAGACTTCAG+TGG + chr8.4:1701768-1701787 MS.gene071722:CDS 45.0%
CATCTAACAATAGTCGGTGG+GGG - chr8.4:1701728-1701747 None:intergenic 45.0%
CCATCTAACAATAGTCGGTG+GGG - chr8.4:1701729-1701748 None:intergenic 45.0%
CTTACTGACATGAAGGACTC+TGG + chr8.4:1701468-1701487 MS.gene071722:CDS 45.0%
GAAGCAATGCAAAGTTACGG+TGG + chr8.4:1701501-1701520 MS.gene071722:CDS 45.0%
GAAGTGAGACTTCAGTGGAA+AGG + chr8.4:1701773-1701792 MS.gene071722:CDS 45.0%
GACCATCTAACAATAGTCGG+TGG - chr8.4:1701731-1701750 None:intergenic 45.0%
GGGGTGAATGCATATGAACT+TGG + chr8.4:1701417-1701436 MS.gene071722:CDS 45.0%
TGGCTATGTTGCGTGAAGAA+CGG - chr8.4:1700634-1700653 None:intergenic 45.0%
TTCAGGAGTTCATCACTGCT+GGG + chr8.4:1701397-1701416 MS.gene071722:intron 45.0%
TTTCAGGAGTTCATCACTGC+TGG + chr8.4:1701396-1701415 MS.gene071722:intron 45.0%
! ACGCGCACAAGGATTTTATG+TGG - chr8.4:1701180-1701199 None:intergenic 45.0%
ACAGAAGAAGAACGCGCACA+AGG - chr8.4:1701191-1701210 None:intergenic 50.0%
TCAGGAGTTCATCACTGCTG+GGG + chr8.4:1701398-1701417 MS.gene071722:intron 50.0%
! CCCCACCGACTATTGTTAGA+TGG + chr8.4:1701726-1701745 MS.gene071722:CDS 50.0%
! GAACTTGGGTGCACTGATGA+GGG + chr8.4:1701432-1701451 MS.gene071722:CDS 50.0%
! GGTGCACTGATGAGGGATTA+AGG + chr8.4:1701439-1701458 MS.gene071722:CDS 50.0%
! TGAACTTGGGTGCACTGATG+AGG + chr8.4:1701431-1701450 MS.gene071722:CDS 50.0%
! CGGTGGGGGTGTACACAAGA+TGG - chr8.4:1701714-1701733 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 1700590 1701843 1700590 ID=MS.gene071722
chr8.4 mRNA 1700590 1701843 1700590 ID=MS.gene071722.t1;Parent=MS.gene071722
chr8.4 exon 1700590 1700712 1700590 ID=MS.gene071722.t1.exon1;Parent=MS.gene071722.t1
chr8.4 CDS 1700590 1700712 1700590 ID=cds.MS.gene071722.t1;Parent=MS.gene071722.t1
chr8.4 exon 1701402 1701560 1701402 ID=MS.gene071722.t1.exon2;Parent=MS.gene071722.t1
chr8.4 CDS 1701402 1701560 1701402 ID=cds.MS.gene071722.t1;Parent=MS.gene071722.t1
chr8.4 exon 1701671 1701843 1701671 ID=MS.gene071722.t1.exon3;Parent=MS.gene071722.t1
chr8.4 CDS 1701671 1701843 1701671 ID=cds.MS.gene071722.t1;Parent=MS.gene071722.t1
Gene Sequence

>MS.gene071722

CAGTGTGGATCACATGACTCAATGTCATCTAATGATGAGTACCGTTCTTCACGCAACATAGCCATCACCTTGTTCAGGAGATATAGGAATTTCACTGATCGTGGTGGAGGTGACAATCTAAAAGTATGTCCACTGCCTCTAGCATCATAATTGAAAATATGCAACTATCAAATTTTAAGAGTTTGAAAAATTATTTCTGATTTTCAAATGAATTGTACACCAGTTGATATACACGTTGTACGTTGTTATAATAGGCAGACTCAAATTGTAATGAGATTGATATTTTGCAGTTCAACCCCATTTTTATGCATTCCTTATAGTTTTGAGCTGGTTTAATTAAGTGTAATAGGATTGCACATTGTATCATGCCGAATTTGAACTCTCTAATTAGTTCTTTGGCAAAGCAATAGGAAACTCTATGTTTGAATTTGTGGTGATTTTGGAAAAATCCAACGACACTGTGATCTTGTTGAATCTTCAAATGGCAACTTTTGCGAAAATCACGTTGGCAATATGTATGCCACATTCACGGTCAATGTAAACATGCACTTATAGACACTTTGTTAAGTTAGATTATATTTGATGATCCACATAAAATCCTTGTGCGCGTTCTTCTTCTGTGCTCTTCTATAGTTTCTATTTACTCTTGTTTCTATCTTAACAAATAGTATACAGAACAAACTGATATAGCTAGTTGTCCCTTTCTTGTATAACTCAACATGCTCACTTCACACGCTTCCTTTACACCTTTTGCTAGTTGGTTCTTTCCCTCTATATTCCAATAAGAGGTGATTTACGTTTTTCATTTTCAGGAGTTCATCACTGCTGGGGTGAATGCATATGAACTTGGGTGCACTGATGAGGGATTAAGGAAAGAGCTTACTGACATGAAGGACTCTGGCTTTGAAATTGAAGCAATGCAAAGTTACGGTGGGAGCACAAGCTTGAAATCTAAAATCATATCTAATGAGGTCTATGATTCTAATCACTTCTTACTTTGTTGCTACAGATGATTTTCTGAAGTCATGAATTCAGCTAGATATCGAGTTACCTTATTTGGCATGTTTTTTGTGTGTTGCAGATTGATGAGTGCATTCTGTGGTTAAGCATTATATTCATCACCATCTTGTGTACACCCCCACCGACTATTGTTAGATGGTCATCAACAACTCCTGTGTCAGATGAAGTGAGACTTCAGTGGAAAGGATTTTGTGCTCTCATAGCAAATGCATATTTTATGAAGGGAATGGCTTG

Protein sequence

>MS.gene071722.t1

QCGSHDSMSSNDEYRSSRNIAITLFRRYRNFTDRGGGDNLKEFITAGVNAYELGCTDEGLRKELTDMKDSGFEIEAMQSYGGSTSLKSKIISNEIDECILWLSIIFITILCTPPPTIVRWSSTTPVSDEVRLQWKGFCALIANAYFMKGMA