Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065378.t1 | XP_003624482.1 | 92.1 | 76 | 5 | 1 | 37 | 111 | 36 | 111 | 3.90E-10 | 74.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065378.t1 | Q2HUL0 | 92.1 | 76 | 5 | 1 | 37 | 111 | 36 | 111 | 2.8e-10 | 74.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene065378 | 0.831563 | 1.56E-55 | -1.69E-46 |
| MS.gene049297 | MS.gene065378 | 0.815005 | 1.15E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene065378 | 0.830925 | 2.23E-55 | -1.69E-46 |
| MS.gene049915 | MS.gene065378 | 0.818037 | 2.42E-52 | -1.69E-46 |
| MS.gene050277 | MS.gene065378 | 0.828681 | 7.85E-55 | -1.69E-46 |
| MS.gene050330 | MS.gene065378 | -0.808852 | 2.53E-50 | -1.69E-46 |
| MS.gene050438 | MS.gene065378 | 0.800277 | 1.55E-48 | -1.69E-46 |
| MS.gene050558 | MS.gene065378 | 0.803599 | 3.23E-49 | -1.69E-46 |
| MS.gene050586 | MS.gene065378 | 0.812075 | 5.09E-51 | -1.69E-46 |
| MS.gene051349 | MS.gene065378 | 0.820686 | 6.02E-53 | -1.69E-46 |
| MS.gene051626 | MS.gene065378 | 0.805177 | 1.51E-49 | -1.69E-46 |
| MS.gene051633 | MS.gene065378 | 0.82339 | 1.42E-53 | -1.69E-46 |
| MS.gene051662 | MS.gene065378 | 0.836016 | 1.20E-56 | -1.69E-46 |
| MS.gene051663 | MS.gene065378 | 0.813837 | 2.09E-51 | -1.69E-46 |
| MS.gene051789 | MS.gene065378 | 0.835872 | 1.30E-56 | -1.69E-46 |
| MS.gene052178 | MS.gene065378 | 0.806469 | 8.10E-50 | -1.69E-46 |
| MS.gene052256 | MS.gene065378 | 0.818437 | 1.96E-52 | -1.69E-46 |
| MS.gene052286 | MS.gene065378 | 0.813116 | 3.01E-51 | -1.69E-46 |
| MS.gene052302 | MS.gene065378 | 0.841285 | 5.21E-58 | -1.69E-46 |
| MS.gene052309 | MS.gene065378 | -0.815063 | 1.12E-51 | -1.69E-46 |
| MS.gene052376 | MS.gene065378 | 0.835878 | 1.30E-56 | -1.69E-46 |
| MS.gene052600 | MS.gene065378 | 0.826918 | 2.08E-54 | -1.69E-46 |
| MS.gene052967 | MS.gene065378 | 0.80884 | 2.54E-50 | -1.69E-46 |
| MS.gene053074 | MS.gene065378 | 0.802765 | 4.80E-49 | -1.69E-46 |
| MS.gene053132 | MS.gene065378 | 0.80243 | 5.63E-49 | -1.69E-46 |
| MS.gene053437 | MS.gene065378 | 0.813637 | 2.31E-51 | -1.69E-46 |
| MS.gene053630 | MS.gene065378 | 0.828478 | 8.79E-55 | -1.69E-46 |
| MS.gene053836 | MS.gene065378 | 0.841243 | 5.35E-58 | -1.69E-46 |
| MS.gene054667 | MS.gene065378 | 0.822579 | 2.20E-53 | -1.69E-46 |
| MS.gene054942 | MS.gene065378 | 0.819915 | 9.04E-53 | -1.69E-46 |
| MS.gene055133 | MS.gene065378 | 0.859689 | 3.48E-63 | -1.69E-46 |
| MS.gene055207 | MS.gene065378 | 0.817734 | 2.83E-52 | -1.69E-46 |
| MS.gene055422 | MS.gene065378 | 0.810328 | 1.22E-50 | -1.69E-46 |
| MS.gene055509 | MS.gene065378 | 0.803028 | 4.24E-49 | -1.69E-46 |
| MS.gene055712 | MS.gene065378 | 0.815967 | 7.05E-52 | -1.69E-46 |
| MS.gene056069 | MS.gene065378 | -0.84137 | 4.95E-58 | -1.69E-46 |
| MS.gene057260 | MS.gene065378 | 0.832893 | 7.29E-56 | -1.69E-46 |
| MS.gene057439 | MS.gene065378 | 0.83579 | 1.37E-56 | -1.69E-46 |
| MS.gene057754 | MS.gene065378 | 0.838053 | 3.61E-57 | -1.69E-46 |
| MS.gene058182 | MS.gene065378 | 0.822485 | 2.31E-53 | -1.69E-46 |
| MS.gene058201 | MS.gene065378 | 0.810869 | 9.30E-51 | -1.69E-46 |
| MS.gene058749 | MS.gene065378 | 0.811355 | 7.30E-51 | -1.69E-46 |
| MS.gene058833 | MS.gene065378 | 0.836027 | 1.19E-56 | -1.69E-46 |
| MS.gene058845 | MS.gene065378 | 0.808772 | 2.63E-50 | -1.69E-46 |
| MS.gene058906 | MS.gene065378 | 0.832686 | 8.21E-56 | -1.69E-46 |
| MS.gene059228 | MS.gene065378 | 0.820721 | 5.90E-53 | -1.69E-46 |
| MS.gene059518 | MS.gene065378 | 0.811833 | 5.75E-51 | -1.69E-46 |
| MS.gene059894 | MS.gene065378 | 0.823052 | 1.70E-53 | -1.69E-46 |
| MS.gene059899 | MS.gene065378 | 0.816348 | 5.79E-52 | -1.69E-46 |
| MS.gene06001 | MS.gene065378 | 0.810546 | 1.09E-50 | -1.69E-46 |
| MS.gene060165 | MS.gene065378 | 0.811439 | 7.00E-51 | -1.69E-46 |
| MS.gene060476 | MS.gene065378 | 0.861674 | 8.69E-64 | -1.69E-46 |
| MS.gene060619 | MS.gene065378 | 0.801542 | 8.57E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene065378 | 0.800561 | 1.36E-48 | -1.69E-46 |
| MS.gene061113 | MS.gene065378 | 0.855037 | 8.26E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065378.t1 | MTR_7g083830 | 94.059 | 101 | 5 | 1 | 12 | 111 | 11 | 111 | 2.60e-44 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTACTTGCCTTTGTCTTTG+TGG | 0.276690 | 7.1:+21233464 | MS.gene065378:CDS |
| GTCTTCTAGTTCAACTAGAT+TGG | 0.332346 | 7.1:+21233683 | MS.gene065378:CDS |
| CCTGAACTTGGTGCCGGTGC+TGG | 0.371358 | 7.1:-21233654 | None:intergenic |
| GTTCCTTAGTATTATTGCTT+AGG | 0.374123 | 7.1:+21233743 | MS.gene065378:CDS |
| CCAGCACCGGCACCAAGTTC+AGG | 0.417982 | 7.1:+21233654 | MS.gene065378:CDS |
| CTCTGCGTCATAAGGAGCTT+CGG | 0.441161 | 7.1:-21233622 | None:intergenic |
| GCAGATGCCTCTGCGTCATA+AGG | 0.465185 | 7.1:-21233630 | None:intergenic |
| CCTGGAGAAGCTGAGATTGT+TGG | 0.468944 | 7.1:-21233537 | None:intergenic |
| CCAACAATCTCAGCTTCTCC+AGG | 0.509863 | 7.1:+21233537 | MS.gene065378:CDS |
| ATACCTAAGCAATAATACTA+AGG | 0.514824 | 7.1:-21233746 | None:intergenic |
| GGAGCTTCGGATGAACTCAT+TGG | 0.517734 | 7.1:-21233609 | None:intergenic |
| AAAGACAAAGGCAAGTAACT+TGG | 0.522688 | 7.1:-21233460 | None:intergenic |
| ATATCTGGAGAAGTCACATA+AGG | 0.536171 | 7.1:-21233567 | None:intergenic |
| TTGGATTGTGCAGCTGCTGT+TGG | 0.546023 | 7.1:+21233702 | MS.gene065378:CDS |
| AGAGGCATCTGCACCAGCAC+CGG | 0.546843 | 7.1:+21233641 | MS.gene065378:CDS |
| GACAATTACCACAAAGACAA+AGG | 0.577039 | 7.1:-21233472 | None:intergenic |
| TCGGATGAACTCATTGGAGT+AGG | 0.578139 | 7.1:-21233603 | None:intergenic |
| GTCACATAAGGTAAAACACC+TGG | 0.582662 | 7.1:-21233555 | None:intergenic |
| AGCTGAGATTGTTGGCTCAG+TGG | 0.602941 | 7.1:-21233529 | None:intergenic |
| CGAAGCTCCTTATGACGCAG+AGG | 0.672283 | 7.1:+21233623 | MS.gene065378:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GGAAAAAATGAAGAAATATC+TGG | - | chr7.1:21233585-21233604 | None:intergenic | 25.0% |
| AAAGACAAAGGCAAGTAACT+TGG | - | chr7.1:21233463-21233482 | None:intergenic | 35.0% | |
| ATATCTGGAGAAGTCACATA+AGG | - | chr7.1:21233570-21233589 | None:intergenic | 35.0% | |
| GACAATTACCACAAAGACAA+AGG | - | chr7.1:21233475-21233494 | None:intergenic | 35.0% | |
| !! | GTCTTCTAGTTCAACTAGAT+TGG | + | chr7.1:21233683-21233702 | MS.gene065378:CDS | 35.0% |
| GTCACATAAGGTAAAACACC+TGG | - | chr7.1:21233558-21233577 | None:intergenic | 40.0% | |
| GTTACTTGCCTTTGTCTTTG+TGG | + | chr7.1:21233464-21233483 | MS.gene065378:CDS | 40.0% | |
| ! | GAAGACTTTTCTCCTGAACT+TGG | - | chr7.1:21233669-21233688 | None:intergenic | 40.0% |
| TCGGATGAACTCATTGGAGT+AGG | - | chr7.1:21233606-21233625 | None:intergenic | 45.0% | |
| !!! | TTTTCTCCTGAACTTGGTGC+CGG | - | chr7.1:21233663-21233682 | None:intergenic | 45.0% |
| CCAACAATCTCAGCTTCTCC+AGG | + | chr7.1:21233537-21233556 | MS.gene065378:CDS | 50.0% | |
| CCTGGAGAAGCTGAGATTGT+TGG | - | chr7.1:21233540-21233559 | None:intergenic | 50.0% | |
| CTCTGCGTCATAAGGAGCTT+CGG | - | chr7.1:21233625-21233644 | None:intergenic | 50.0% | |
| GGAGCTTCGGATGAACTCAT+TGG | - | chr7.1:21233612-21233631 | None:intergenic | 50.0% | |
| TTGGATTGTGCAGCTGCTGT+TGG | + | chr7.1:21233702-21233721 | MS.gene065378:CDS | 50.0% | |
| !! | AGCTGAGATTGTTGGCTCAG+TGG | - | chr7.1:21233532-21233551 | None:intergenic | 50.0% |
| CGAAGCTCCTTATGACGCAG+AGG | + | chr7.1:21233623-21233642 | MS.gene065378:CDS | 55.0% | |
| GCAGATGCCTCTGCGTCATA+AGG | - | chr7.1:21233633-21233652 | None:intergenic | 55.0% | |
| !! | AGAGGCATCTGCACCAGCAC+CGG | + | chr7.1:21233641-21233660 | MS.gene065378:CDS | 60.0% |
| !! | CCAGCACCGGCACCAAGTTC+AGG | + | chr7.1:21233654-21233673 | MS.gene065378:CDS | 65.0% |
| !! | CCTGAACTTGGTGCCGGTGC+TGG | - | chr7.1:21233657-21233676 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 21233429 | 21233764 | 21233429 | ID=MS.gene065378 |
| chr7.1 | mRNA | 21233429 | 21233764 | 21233429 | ID=MS.gene065378.t1;Parent=MS.gene065378 |
| chr7.1 | exon | 21233429 | 21233764 | 21233429 | ID=MS.gene065378.t1.exon1;Parent=MS.gene065378.t1 |
| chr7.1 | CDS | 21233429 | 21233764 | 21233429 | ID=cds.MS.gene065378.t1;Parent=MS.gene065378.t1 |
| Gene Sequence |
| Protein sequence |
