Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067363.t1 | XP_013443474.1 | 98.6 | 349 | 5 | 0 | 1 | 349 | 1 | 349 | 2.10E-182 | 648.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067363.t1 | Q9SUV2 | 79.7 | 349 | 67 | 2 | 2 | 350 | 6 | 350 | 2.2e-148 | 526.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067363.t1 | A0A072TIW6 | 98.6 | 349 | 5 | 0 | 1 | 349 | 1 | 349 | 1.5e-182 | 648.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene067363 | -0.805035 | 1.62E-49 | -1.69E-46 |
| MS.gene049181 | MS.gene067363 | 0.800761 | 1.24E-48 | -1.69E-46 |
| MS.gene049281 | MS.gene067363 | 0.836451 | 9.29E-57 | -1.69E-46 |
| MS.gene049312 | MS.gene067363 | 0.803755 | 3.00E-49 | -1.69E-46 |
| MS.gene049320 | MS.gene067363 | -0.810298 | 1.24E-50 | -1.69E-46 |
| MS.gene050170 | MS.gene067363 | -0.813738 | 2.20E-51 | -1.69E-46 |
| MS.gene050381 | MS.gene067363 | 0.811258 | 7.66E-51 | -1.69E-46 |
| MS.gene050496 | MS.gene067363 | -0.827014 | 1.98E-54 | -1.69E-46 |
| MS.gene050504 | MS.gene067363 | -0.821701 | 3.51E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene067363 | -0.822225 | 2.65E-53 | -1.69E-46 |
| MS.gene050675 | MS.gene067363 | -0.800055 | 1.72E-48 | -1.69E-46 |
| MS.gene050699 | MS.gene067363 | -0.813844 | 2.08E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene067363 | -0.813288 | 2.76E-51 | -1.69E-46 |
| MS.gene050871 | MS.gene067363 | -0.811219 | 7.81E-51 | -1.69E-46 |
| MS.gene050883 | MS.gene067363 | 0.806424 | 8.28E-50 | -1.69E-46 |
| MS.gene051044 | MS.gene067363 | 0.832576 | 8.74E-56 | -1.69E-46 |
| MS.gene051364 | MS.gene067363 | 0.806007 | 1.01E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene067363 | -0.80682 | 6.83E-50 | -1.69E-46 |
| MS.gene053203 | MS.gene067363 | -0.803414 | 3.53E-49 | -1.69E-46 |
| MS.gene053549 | MS.gene067363 | 0.821056 | 4.95E-53 | -1.69E-46 |
| MS.gene053826 | MS.gene067363 | 0.806416 | 8.31E-50 | -1.69E-46 |
| MS.gene054056 | MS.gene067363 | 0.815936 | 7.16E-52 | -1.69E-46 |
| MS.gene05470 | MS.gene067363 | -0.836268 | 1.03E-56 | -1.69E-46 |
| MS.gene054954 | MS.gene067363 | -0.808539 | 2.95E-50 | -1.69E-46 |
| MS.gene055362 | MS.gene067363 | -0.820965 | 5.19E-53 | -1.69E-46 |
| MS.gene055502 | MS.gene067363 | -0.8036 | 3.23E-49 | -1.69E-46 |
| MS.gene055776 | MS.gene067363 | -0.808116 | 3.63E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene067363 | -0.826557 | 2.54E-54 | -1.69E-46 |
| MS.gene055942 | MS.gene067363 | 0.813561 | 2.41E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene067363 | -0.831391 | 1.71E-55 | -1.69E-46 |
| MS.gene056550 | MS.gene067363 | 0.809551 | 1.79E-50 | -1.69E-46 |
| MS.gene056761 | MS.gene067363 | 0.823767 | 1.16E-53 | -1.69E-46 |
| MS.gene057014 | MS.gene067363 | 0.813041 | 3.13E-51 | -1.69E-46 |
| MS.gene058268 | MS.gene067363 | 0.809706 | 1.66E-50 | -1.69E-46 |
| MS.gene058269 | MS.gene067363 | 0.808142 | 3.58E-50 | -1.69E-46 |
| MS.gene058334 | MS.gene067363 | 0.831874 | 1.30E-55 | -1.69E-46 |
| MS.gene059022 | MS.gene067363 | -0.832519 | 9.03E-56 | -1.69E-46 |
| MS.gene059677 | MS.gene067363 | -0.820018 | 8.56E-53 | -1.69E-46 |
| MS.gene059718 | MS.gene067363 | -0.803219 | 3.87E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene067363 | -0.822454 | 2.35E-53 | -1.69E-46 |
| MS.gene060595 | MS.gene067363 | 0.826622 | 2.45E-54 | -1.69E-46 |
| MS.gene060703 | MS.gene067363 | -0.820186 | 7.83E-53 | -1.69E-46 |
| MS.gene060762 | MS.gene067363 | -0.802749 | 4.84E-49 | -1.69E-46 |
| MS.gene060778 | MS.gene067363 | -0.800368 | 1.49E-48 | -1.69E-46 |
| MS.gene06077 | MS.gene067363 | -0.81473 | 1.33E-51 | -1.69E-46 |
| MS.gene060982 | MS.gene067363 | -0.803939 | 2.74E-49 | -1.69E-46 |
| MS.gene061044 | MS.gene067363 | 0.800723 | 1.26E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067363.t1 | MTR_0011s0320 | 98.567 | 349 | 5 | 0 | 1 | 349 | 1 | 349 | 0.0 | 699 |
| MS.gene067363.t1 | MTR_4g114360 | 83.143 | 350 | 54 | 2 | 1 | 350 | 1 | 345 | 0.0 | 590 |
| MS.gene067363.t1 | MTR_3g107470 | 76.791 | 349 | 76 | 2 | 2 | 350 | 6 | 349 | 0.0 | 552 |
| MS.gene067363.t1 | MTR_0662s0010 | 87.004 | 277 | 36 | 0 | 1 | 277 | 1 | 277 | 1.31e-175 | 488 |
| MS.gene067363.t1 | MTR_7g110380 | 46.608 | 339 | 174 | 3 | 9 | 346 | 10 | 342 | 3.94e-110 | 326 |
| MS.gene067363.t1 | MTR_4g123900 | 46.313 | 339 | 174 | 4 | 9 | 340 | 6 | 343 | 1.43e-101 | 305 |
| MS.gene067363.t1 | MTR_4g123900 | 50.523 | 287 | 140 | 2 | 9 | 294 | 6 | 291 | 6.33e-97 | 290 |
| MS.gene067363.t1 | MTR_2g070400 | 41.818 | 330 | 180 | 4 | 13 | 340 | 10 | 329 | 2.39e-83 | 256 |
| MS.gene067363.t1 | MTR_3g070000 | 32.997 | 297 | 183 | 5 | 18 | 305 | 29 | 318 | 9.14e-37 | 135 |
| MS.gene067363.t1 | MTR_5g081710 | 32.770 | 296 | 189 | 4 | 16 | 305 | 11 | 302 | 4.19e-36 | 133 |
| MS.gene067363.t1 | MTR_3g462070 | 31.333 | 300 | 196 | 4 | 12 | 305 | 8 | 303 | 1.66e-34 | 129 |
| MS.gene067363.t1 | MTR_8g090130 | 35.135 | 296 | 178 | 7 | 18 | 305 | 16 | 305 | 8.11e-34 | 127 |
| MS.gene067363.t1 | MTR_4g018760 | 30.928 | 291 | 191 | 4 | 21 | 305 | 20 | 306 | 3.12e-31 | 120 |
| MS.gene067363.t1 | MTR_1g030320 | 24.159 | 327 | 236 | 8 | 21 | 341 | 21 | 341 | 4.56e-28 | 112 |
| MS.gene067363.t1 | MTR_4g101860 | 28.477 | 302 | 206 | 6 | 18 | 313 | 57 | 354 | 1.09e-27 | 111 |
| MS.gene067363.t1 | MTR_3g072380 | 23.511 | 319 | 234 | 6 | 21 | 334 | 22 | 335 | 1.15e-26 | 108 |
| MS.gene067363.t1 | MTR_8g016090 | 27.246 | 345 | 228 | 10 | 10 | 338 | 165 | 502 | 1.20e-22 | 99.0 |
| MS.gene067363.t1 | MTR_2g094640 | 26.254 | 339 | 211 | 13 | 25 | 345 | 22 | 339 | 8.22e-20 | 89.4 |
| MS.gene067363.t1 | MTR_4g058860 | 26.176 | 340 | 216 | 15 | 25 | 349 | 22 | 341 | 1.91e-18 | 85.5 |
| MS.gene067363.t1 | MTR_8g016090 | 28.996 | 269 | 170 | 9 | 84 | 338 | 191 | 452 | 1.40e-16 | 80.9 |
| MS.gene067363.t1 | MTR_3g094270 | 25.641 | 273 | 163 | 9 | 83 | 339 | 73 | 321 | 1.15e-14 | 74.3 |
| MS.gene067363.t1 | MTR_3g094260 | 26.493 | 268 | 167 | 9 | 83 | 339 | 73 | 321 | 1.17e-13 | 71.6 |
| MS.gene067363.t1 | MTR_2g038410 | 24.294 | 354 | 241 | 11 | 4 | 348 | 45 | 380 | 1.23e-13 | 71.6 |
| MS.gene067363.t1 | MTR_8g016090 | 25.414 | 181 | 129 | 3 | 10 | 186 | 165 | 343 | 6.63e-12 | 66.6 |
| MS.gene067363.t1 | MTR_3g072380 | 24.405 | 168 | 123 | 4 | 21 | 187 | 22 | 186 | 1.25e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067363.t1 | AT4G32390 | 79.656 | 349 | 67 | 2 | 2 | 350 | 6 | 350 | 0.0 | 570 |
| MS.gene067363.t1 | AT2G25520 | 78.510 | 349 | 68 | 3 | 2 | 350 | 6 | 347 | 0.0 | 557 |
| MS.gene067363.t1 | AT5G25400 | 77.077 | 349 | 75 | 2 | 2 | 350 | 6 | 349 | 0.0 | 557 |
| MS.gene067363.t1 | AT5G11230 | 74.499 | 349 | 86 | 2 | 2 | 350 | 6 | 351 | 0.0 | 531 |
| MS.gene067363.t1 | AT3G17430 | 46.082 | 319 | 170 | 2 | 25 | 342 | 23 | 340 | 1.00e-101 | 305 |
| MS.gene067363.t1 | AT3G17430 | 46.082 | 319 | 170 | 2 | 25 | 342 | 23 | 340 | 1.00e-101 | 305 |
| MS.gene067363.t1 | AT1G48230 | 47.853 | 326 | 159 | 4 | 25 | 347 | 23 | 340 | 8.47e-100 | 300 |
| MS.gene067363.t1 | AT3G14410 | 41.298 | 339 | 194 | 3 | 4 | 340 | 5 | 340 | 1.61e-82 | 254 |
| MS.gene067363.t1 | AT1G53660 | 41.049 | 324 | 183 | 4 | 5 | 326 | 8 | 325 | 1.09e-81 | 252 |
| MS.gene067363.t1 | AT1G53660 | 41.049 | 324 | 183 | 4 | 5 | 326 | 26 | 343 | 1.22e-81 | 252 |
| MS.gene067363.t1 | AT1G48230 | 47.273 | 275 | 134 | 4 | 76 | 347 | 1 | 267 | 4.17e-78 | 241 |
| MS.gene067363.t1 | AT1G53660 | 41.503 | 306 | 166 | 5 | 33 | 336 | 2 | 296 | 3.61e-77 | 239 |
| MS.gene067363.t1 | AT1G53660 | 41.296 | 247 | 133 | 4 | 91 | 336 | 1 | 236 | 8.50e-58 | 187 |
| MS.gene067363.t1 | AT1G53660 | 41.791 | 201 | 116 | 1 | 5 | 204 | 26 | 226 | 3.01e-53 | 176 |
| MS.gene067363.t1 | AT3G11320 | 32.653 | 294 | 188 | 4 | 18 | 305 | 15 | 304 | 1.53e-34 | 129 |
| MS.gene067363.t1 | AT5G05820 | 32.993 | 294 | 187 | 4 | 18 | 305 | 15 | 304 | 4.61e-34 | 127 |
| MS.gene067363.t1 | AT1G21870 | 27.835 | 291 | 198 | 7 | 21 | 305 | 22 | 306 | 2.28e-31 | 121 |
| MS.gene067363.t1 | AT5G04160 | 30.744 | 309 | 204 | 5 | 3 | 305 | 1 | 305 | 9.14e-29 | 113 |
| MS.gene067363.t1 | AT3G10290 | 29.703 | 303 | 203 | 4 | 9 | 305 | 53 | 351 | 3.08e-28 | 113 |
| MS.gene067363.t1 | AT1G77610 | 25.649 | 308 | 216 | 7 | 3 | 305 | 1 | 300 | 1.32e-27 | 111 |
| MS.gene067363.t1 | AT1G12500 | 28.939 | 311 | 207 | 9 | 3 | 305 | 48 | 352 | 3.16e-27 | 110 |
| MS.gene067363.t1 | AT2G28315 | 28.358 | 335 | 212 | 14 | 25 | 348 | 22 | 339 | 7.17e-22 | 95.1 |
| MS.gene067363.t1 | AT1G06470 | 24.047 | 341 | 247 | 5 | 11 | 339 | 72 | 412 | 1.29e-20 | 92.4 |
| MS.gene067363.t1 | AT1G06470 | 24.047 | 341 | 247 | 5 | 11 | 339 | 72 | 412 | 1.29e-20 | 92.4 |
| MS.gene067363.t1 | AT1G06470 | 24.047 | 341 | 247 | 5 | 11 | 339 | 72 | 412 | 1.29e-20 | 92.4 |
| MS.gene067363.t1 | AT1G06470 | 24.047 | 341 | 247 | 5 | 11 | 339 | 72 | 412 | 1.29e-20 | 92.4 |
| MS.gene067363.t1 | AT2G28315 | 29.900 | 301 | 184 | 13 | 25 | 314 | 22 | 306 | 4.61e-19 | 87.0 |
| MS.gene067363.t1 | AT2G30460 | 25.321 | 312 | 201 | 11 | 42 | 338 | 37 | 331 | 1.44e-15 | 77.4 |
| MS.gene067363.t1 | AT2G30460 | 25.321 | 312 | 201 | 11 | 42 | 338 | 37 | 331 | 1.44e-15 | 77.4 |
| MS.gene067363.t1 | AT2G30460 | 25.658 | 304 | 194 | 11 | 48 | 336 | 22 | 308 | 5.19e-15 | 75.5 |
| MS.gene067363.t1 | AT2G30460 | 25.658 | 304 | 194 | 11 | 48 | 336 | 22 | 308 | 5.19e-15 | 75.5 |
| MS.gene067363.t1 | AT2G30460 | 25.658 | 304 | 194 | 11 | 48 | 336 | 22 | 308 | 5.19e-15 | 75.5 |
| MS.gene067363.t1 | AT1G06890 | 24.679 | 312 | 203 | 11 | 42 | 338 | 37 | 331 | 5.32e-15 | 75.5 |
| MS.gene067363.t1 | AT1G06890 | 24.679 | 312 | 203 | 11 | 42 | 338 | 37 | 331 | 5.32e-15 | 75.5 |
| MS.gene067363.t1 | AT1G06890 | 24.679 | 312 | 203 | 11 | 42 | 338 | 37 | 331 | 5.75e-15 | 75.5 |
| MS.gene067363.t1 | AT1G06890 | 24.679 | 312 | 203 | 11 | 42 | 338 | 37 | 331 | 6.48e-15 | 75.5 |
| MS.gene067363.t1 | AT5G57100 | 27.362 | 307 | 185 | 12 | 11 | 303 | 66 | 348 | 8.09e-12 | 66.2 |
Find 71 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGATCATTGTTAGTGATAT+TGG | 0.251388 | 2.3:-36087260 | None:intergenic |
| CAATAGGGACCACTGATTTA+AGG | 0.269325 | 2.3:-36087367 | None:intergenic |
| GTGTCTAAAGACATTGAAAT+TGG | 0.278188 | 2.3:-36087341 | None:intergenic |
| CTACATTGCTCCTTGTTGTT+TGG | 0.286555 | 2.3:+36087721 | MS.gene067363:CDS |
| CACTTTATTCTCTCTCCCTT+TGG | 0.309209 | 2.3:+36087393 | MS.gene067363:CDS |
| CTGTAAGTTGGTGGCACTTA+AGG | 0.314139 | 2.3:+36088033 | MS.gene067363:CDS |
| GTTACACCGCTCAATTTGAT+TGG | 0.363003 | 2.3:+36087971 | MS.gene067363:CDS |
| GGTGTCGTTGTAGCTGCTTA+TGG | 0.387943 | 2.3:+36087566 | MS.gene067363:CDS |
| ATGTAGTAAAGCGAGGTTAT+AGG | 0.388406 | 2.3:-36087704 | None:intergenic |
| CTTCTGAGGCACAAAAGATC+AGG | 0.389412 | 2.3:+36088056 | MS.gene067363:CDS |
| CCGCTCAATTTGATTGGTTA+CGG | 0.398421 | 2.3:+36087977 | MS.gene067363:CDS |
| GGATCATTGTTAGTGATATT+GGG | 0.411771 | 2.3:-36087259 | None:intergenic |
| GAAGCCAAGTTTGATGTCTT+TGG | 0.416149 | 2.3:+36087590 | MS.gene067363:CDS |
| CTTAAATCAGTGGTCCCTAT+TGG | 0.416269 | 2.3:+36087368 | MS.gene067363:CDS |
| CCGTGGCCGTGTACTCCATC+GGG | 0.416480 | 2.3:+36087480 | MS.gene067363:CDS |
| GCTTTGACGATGAATGTTGC+TGG | 0.428804 | 2.3:+36087893 | MS.gene067363:CDS |
| AGAGAATAAAGTGCACCAAT+AGG | 0.429914 | 2.3:-36087383 | None:intergenic |
| CCCGTGGCCGTGTACTCCAT+CGG | 0.433129 | 2.3:+36087479 | MS.gene067363:CDS |
| TTCTGAGGCTGGGAAATTGT+TGG | 0.436034 | 2.3:+36088096 | MS.gene067363:CDS |
| GTACAAGGAAGAATGAGAAT+AGG | 0.438911 | 2.3:+36088149 | MS.gene067363:CDS |
| TTATTATAATCACTGTAAGT+TGG | 0.439379 | 2.3:+36088021 | MS.gene067363:CDS |
| TTGCTGGTGTTGTGAAGGAT+TGG | 0.445415 | 2.3:+36087909 | MS.gene067363:CDS |
| AATAGAAACCATGTTGATCA+TGG | 0.447620 | 2.3:-36087537 | None:intergenic |
| ACAAAAGATCAGGGTTCAGC+AGG | 0.447972 | 2.3:+36088066 | MS.gene067363:CDS |
| CATACACATATGTAGCCATA+TGG | 0.453282 | 2.3:+36087165 | MS.gene067363:CDS |
| CAGGAAGAAGATTCTGAGGC+TGG | 0.457100 | 2.3:+36088085 | MS.gene067363:CDS |
| GAGAATAAAGTGCACCAATA+GGG | 0.463814 | 2.3:-36087382 | None:intergenic |
| GTCGTTTCCTTGGTTCGTCA+TGG | 0.465506 | 2.3:+36087751 | MS.gene067363:CDS |
| TGTCTAAAGACATTGAAATT+GGG | 0.487670 | 2.3:-36087340 | None:intergenic |
| TGGTGTTACGCTGCAGCTCA+TGG | 0.494202 | 2.3:+36087610 | MS.gene067363:CDS |
| GAAACTTAAGAAAATCCATA+TGG | 0.505718 | 2.3:-36087180 | None:intergenic |
| AACAAACATGGCAGTATCAG+AGG | 0.506552 | 2.3:+36087118 | None:intergenic |
| TCATGGAGTATCCTTTGTTG+AGG | 0.511788 | 2.3:+36087768 | MS.gene067363:CDS |
| ATTGTTAGTGATATTGGGAA+TGG | 0.515101 | 2.3:-36087254 | None:intergenic |
| GTAAGCAAGGGAGGAACAAA+AGG | 0.515623 | 2.3:-36087288 | None:intergenic |
| AACACCAAAGACATCAAACT+TGG | 0.526425 | 2.3:-36087594 | None:intergenic |
| AATTGTTGGAAGAGAGAGAT+GGG | 0.526474 | 2.3:+36088110 | MS.gene067363:CDS |
| AAGCAGAGTTTGAGAACCAA+AGG | 0.537705 | 2.3:-36087409 | None:intergenic |
| TTATAATCACTGTAAGTTGG+TGG | 0.541314 | 2.3:+36088024 | MS.gene067363:CDS |
| TTTGCTTGTTGGGAAGACTT+CGG | 0.544452 | 2.3:+36087871 | MS.gene067363:CDS |
| AACATGGTTTCTATTTCACT+TGG | 0.559499 | 2.3:+36087545 | MS.gene067363:CDS |
| CATGGAGTATCCTTTGTTGA+GGG | 0.562243 | 2.3:+36087769 | MS.gene067363:CDS |
| CCCGATGGAGTACACGGCCA+CGG | 0.564177 | 2.3:-36087480 | None:intergenic |
| CCGATGGAGTACACGGCCAC+GGG | 0.570971 | 2.3:-36087479 | None:intergenic |
| AGCGTTTGAAGCCACGAGGT+TGG | 0.575373 | 2.3:+36087637 | MS.gene067363:CDS |
| GGTGGCACTTAAGGCTTCTG+AGG | 0.577244 | 2.3:+36088042 | MS.gene067363:CDS |
| TGTTTACGAGGATGTAAGCA+AGG | 0.581146 | 2.3:-36087301 | None:intergenic |
| AGCAGAGTTTGAGAACCAAA+GGG | 0.583295 | 2.3:-36087408 | None:intergenic |
| AGGAAGAAGATTCTGAGGCT+GGG | 0.583941 | 2.3:+36088086 | MS.gene067363:CDS |
| CTACTTTGAAGATGTTTACG+AGG | 0.591183 | 2.3:-36087313 | None:intergenic |
| GAATGTTGCTGGTGTTGTGA+AGG | 0.593106 | 2.3:+36087904 | MS.gene067363:CDS |
| AGAAACCCCGATGGAGTACA+CGG | 0.604166 | 2.3:-36087486 | None:intergenic |
| AAATTGTTGGAAGAGAGAGA+TGG | 0.605714 | 2.3:+36088109 | MS.gene067363:CDS |
| TTCTGAGGCACAAAAGATCA+GGG | 0.610985 | 2.3:+36088057 | MS.gene067363:CDS |
| CTTGCTTTATCCCTCAACAA+AGG | 0.614249 | 2.3:-36087779 | None:intergenic |
| TTGAATTAAAACCAACCTCG+TGG | 0.618109 | 2.3:-36087648 | None:intergenic |
| GGATACTCCATGACGAACCA+AGG | 0.620318 | 2.3:-36087758 | None:intergenic |
| TACGAGGATGTAAGCAAGGG+AGG | 0.626594 | 2.3:-36087297 | None:intergenic |
| AGACACATACCTTAAATCAG+TGG | 0.633020 | 2.3:+36087358 | MS.gene067363:CDS |
| AAAATGATGAAGAAAGTACA+AGG | 0.636053 | 2.3:+36088134 | MS.gene067363:CDS |
| GCTAAAAGCTCTTATGCCCG+TGG | 0.637331 | 2.3:+36087463 | MS.gene067363:CDS |
| AGGAGCAATGTAGTAAAGCG+AGG | 0.649670 | 2.3:-36087711 | None:intergenic |
| TCAGCAGGAAGAAGATTCTG+AGG | 0.650356 | 2.3:+36088081 | MS.gene067363:CDS |
| CCGTAACCAATCAAATTGAG+CGG | 0.670562 | 2.3:-36087977 | None:intergenic |
| GTTTACGAGGATGTAAGCAA+GGG | 0.670718 | 2.3:-36087300 | None:intergenic |
| AAAAGAAACCATGATCAACA+TGG | 0.670931 | 2.3:+36087529 | MS.gene067363:CDS |
| ACAAACATGGCAGTATCAGA+GGG | 0.674351 | 2.3:+36087119 | None:intergenic |
| GGGAGGAACAAAAGGCCATG+TGG | 0.678631 | 2.3:-36087280 | None:intergenic |
| CTGTAGCGTTTGAAGCCACG+AGG | 0.681815 | 2.3:+36087633 | MS.gene067363:CDS |
| ATCACTAACAATGATCCACA+TGG | 0.695115 | 2.3:+36087265 | MS.gene067363:CDS |
| CGTGGCCGTGTACTCCATCG+GGG | 0.738168 | 2.3:+36087481 | MS.gene067363:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTATAATCACTGTAAGT+TGG | + | chr2.3:36088021-36088040 | MS.gene067363:CDS | 20.0% |
| !!! | AAGTTTGAGTTTTGATTTTT+GGG | + | chr2.3:36087799-36087818 | MS.gene067363:CDS | 20.0% |
| ! | GAAACTTAAGAAAATCCATA+TGG | - | chr2.3:36087183-36087202 | None:intergenic | 25.0% |
| ! | TAGACCAAAAAATGTACAAT+TGG | + | chr2.3:36087231-36087250 | MS.gene067363:CDS | 25.0% |
| ! | TGTCTAAAGACATTGAAATT+GGG | - | chr2.3:36087343-36087362 | None:intergenic | 25.0% |
| !! | AAAATGATGAAGAAAGTACA+AGG | + | chr2.3:36088134-36088153 | MS.gene067363:CDS | 25.0% |
| !! | AAATTCTTTTGAACTCGAAA+GGG | + | chr2.3:36087669-36087688 | MS.gene067363:CDS | 25.0% |
| !!! | AATTGTACATTTTTTGGTCT+AGG | - | chr2.3:36087232-36087251 | None:intergenic | 25.0% |
| !!! | AGTTTTGATTTTTGGGTTTT+TGG | + | chr2.3:36087806-36087825 | MS.gene067363:CDS | 25.0% |
| !!! | CAAGTTTGAGTTTTGATTTT+TGG | + | chr2.3:36087798-36087817 | MS.gene067363:CDS | 25.0% |
| AAAAGAAACCATGATCAACA+TGG | + | chr2.3:36087529-36087548 | MS.gene067363:CDS | 30.0% | |
| AACATGGTTTCTATTTCACT+TGG | + | chr2.3:36087545-36087564 | MS.gene067363:CDS | 30.0% | |
| AATAGAAACCATGTTGATCA+TGG | - | chr2.3:36087540-36087559 | None:intergenic | 30.0% | |
| ATTGTTAGTGATATTGGGAA+TGG | - | chr2.3:36087257-36087276 | None:intergenic | 30.0% | |
| GACAAAAAAACCAAACAACA+AGG | - | chr2.3:36087734-36087753 | None:intergenic | 30.0% | |
| GGATCATTGTTAGTGATATT+GGG | - | chr2.3:36087262-36087281 | None:intergenic | 30.0% | |
| GTGTCTAAAGACATTGAAAT+TGG | - | chr2.3:36087344-36087363 | None:intergenic | 30.0% | |
| TGGATCATTGTTAGTGATAT+TGG | - | chr2.3:36087263-36087282 | None:intergenic | 30.0% | |
| ! | ATTTTCTTGGTCAGTGATTA+AGG | + | chr2.3:36087943-36087962 | MS.gene067363:CDS | 30.0% |
| ! | CAAATTCTTTTGAACTCGAA+AGG | + | chr2.3:36087668-36087687 | MS.gene067363:CDS | 30.0% |
| ! | TTATAATCACTGTAAGTTGG+TGG | + | chr2.3:36088024-36088043 | MS.gene067363:CDS | 30.0% |
| !!! | ATGGCCAATTGTACATTTTT+TGG | - | chr2.3:36087238-36087257 | None:intergenic | 30.0% |
| !!! | TTTTTTAAAAGAAACCCCGA+TGG | - | chr2.3:36087498-36087517 | None:intergenic | 30.0% |
| AAATTGTTGGAAGAGAGAGA+TGG | + | chr2.3:36088109-36088128 | MS.gene067363:CDS | 35.0% | |
| AACACCAAAGACATCAAACT+TGG | - | chr2.3:36087597-36087616 | None:intergenic | 35.0% | |
| AATTGTTGGAAGAGAGAGAT+GGG | + | chr2.3:36088110-36088129 | MS.gene067363:CDS | 35.0% | |
| AGACACATACCTTAAATCAG+TGG | + | chr2.3:36087358-36087377 | MS.gene067363:CDS | 35.0% | |
| AGAGAATAAAGTGCACCAAT+AGG | - | chr2.3:36087386-36087405 | None:intergenic | 35.0% | |
| ATCACTAACAATGATCCACA+TGG | + | chr2.3:36087265-36087284 | MS.gene067363:CDS | 35.0% | |
| ATGTAGTAAAGCGAGGTTAT+AGG | - | chr2.3:36087707-36087726 | None:intergenic | 35.0% | |
| CATACACATATGTAGCCATA+TGG | + | chr2.3:36087165-36087184 | MS.gene067363:CDS | 35.0% | |
| GAGAATAAAGTGCACCAATA+GGG | - | chr2.3:36087385-36087404 | None:intergenic | 35.0% | |
| GTACAAGGAAGAATGAGAAT+AGG | + | chr2.3:36088149-36088168 | MS.gene067363:CDS | 35.0% | |
| TTGAATTAAAACCAACCTCG+TGG | - | chr2.3:36087651-36087670 | None:intergenic | 35.0% | |
| ! | CTACTTTGAAGATGTTTACG+AGG | - | chr2.3:36087316-36087335 | None:intergenic | 35.0% |
| ! | GGTTGCTTATTGCATTTTCT+TGG | + | chr2.3:36087930-36087949 | MS.gene067363:CDS | 35.0% |
| !! | TTGCTGTGTTTTTGCTTGTT+GGG | + | chr2.3:36087861-36087880 | MS.gene067363:CDS | 35.0% |
| !!! | TGGTTTTTTTGTCGTTTCCT+TGG | + | chr2.3:36087741-36087760 | MS.gene067363:CDS | 35.0% |
| AAGCAGAGTTTGAGAACCAA+AGG | - | chr2.3:36087412-36087431 | None:intergenic | 40.0% | |
| AGCAGAGTTTGAGAACCAAA+GGG | - | chr2.3:36087411-36087430 | None:intergenic | 40.0% | |
| CAACAAGCAAAAACACAGCA+AGG | - | chr2.3:36087862-36087881 | None:intergenic | 40.0% | |
| CACTTTATTCTCTCTCCCTT+TGG | + | chr2.3:36087393-36087412 | MS.gene067363:CDS | 40.0% | |
| CCGTAACCAATCAAATTGAG+CGG | - | chr2.3:36087980-36087999 | None:intergenic | 40.0% | |
| CTTAAATCAGTGGTCCCTAT+TGG | + | chr2.3:36087368-36087387 | MS.gene067363:CDS | 40.0% | |
| CTTGCTTTATCCCTCAACAA+AGG | - | chr2.3:36087782-36087801 | None:intergenic | 40.0% | |
| GAAGCCAAGTTTGATGTCTT+TGG | + | chr2.3:36087590-36087609 | MS.gene067363:CDS | 40.0% | |
| GTTACACCGCTCAATTTGAT+TGG | + | chr2.3:36087971-36087990 | MS.gene067363:CDS | 40.0% | |
| GTTTACGAGGATGTAAGCAA+GGG | - | chr2.3:36087303-36087322 | None:intergenic | 40.0% | |
| TGTTTACGAGGATGTAAGCA+AGG | - | chr2.3:36087304-36087323 | None:intergenic | 40.0% | |
| ! | CATGGAGTATCCTTTGTTGA+GGG | + | chr2.3:36087769-36087788 | MS.gene067363:CDS | 40.0% |
| ! | CCGCTCAATTTGATTGGTTA+CGG | + | chr2.3:36087977-36087996 | MS.gene067363:CDS | 40.0% |
| ! | GGTTACGGACTTGCATTTTT+GGG | + | chr2.3:36087992-36088011 | MS.gene067363:CDS | 40.0% |
| ! | GTTACGGACTTGCATTTTTG+GGG | + | chr2.3:36087993-36088012 | MS.gene067363:CDS | 40.0% |
| ! | TCATGGAGTATCCTTTGTTG+AGG | + | chr2.3:36087768-36087787 | MS.gene067363:CDS | 40.0% |
| ! | TGGTTACGGACTTGCATTTT+TGG | + | chr2.3:36087991-36088010 | MS.gene067363:CDS | 40.0% |
| ! | TTTGCTTGTTGGGAAGACTT+CGG | + | chr2.3:36087871-36087890 | MS.gene067363:CDS | 40.0% |
| !! | CAATAGGGACCACTGATTTA+AGG | - | chr2.3:36087370-36087389 | None:intergenic | 40.0% |
| !! | CTACATTGCTCCTTGTTGTT+TGG | + | chr2.3:36087721-36087740 | MS.gene067363:CDS | 40.0% |
| !! | CTTGCTGTGTTTTTGCTTGT+TGG | + | chr2.3:36087860-36087879 | MS.gene067363:CDS | 40.0% |
| !! | TTACGGACTTGCATTTTTGG+GGG | + | chr2.3:36087994-36088013 | MS.gene067363:CDS | 40.0% |
| !! | TTCTGAGGCACAAAAGATCA+GGG | + | chr2.3:36088057-36088076 | MS.gene067363:CDS | 40.0% |
| ACAAAAGATCAGGGTTCAGC+AGG | + | chr2.3:36088066-36088085 | MS.gene067363:CDS | 45.0% | |
| AGGAGCAATGTAGTAAAGCG+AGG | - | chr2.3:36087714-36087733 | None:intergenic | 45.0% | |
| GCTTTGACGATGAATGTTGC+TGG | + | chr2.3:36087893-36087912 | MS.gene067363:CDS | 45.0% | |
| GTAAGCAAGGGAGGAACAAA+AGG | - | chr2.3:36087291-36087310 | None:intergenic | 45.0% | |
| TCAGCAGGAAGAAGATTCTG+AGG | + | chr2.3:36088081-36088100 | MS.gene067363:CDS | 45.0% | |
| !! | AGGAAGAAGATTCTGAGGCT+GGG | + | chr2.3:36088086-36088105 | MS.gene067363:CDS | 45.0% |
| !! | CTGTAAGTTGGTGGCACTTA+AGG | + | chr2.3:36088033-36088052 | MS.gene067363:CDS | 45.0% |
| !! | CTTCTGAGGCACAAAAGATC+AGG | + | chr2.3:36088056-36088075 | MS.gene067363:CDS | 45.0% |
| !! | GAATGTTGCTGGTGTTGTGA+AGG | + | chr2.3:36087904-36087923 | MS.gene067363:CDS | 45.0% |
| !! | TTCTGAGGCTGGGAAATTGT+TGG | + | chr2.3:36088096-36088115 | MS.gene067363:CDS | 45.0% |
| !! | TTGCTGGTGTTGTGAAGGAT+TGG | + | chr2.3:36087909-36087928 | MS.gene067363:CDS | 45.0% |
| AGAAACCCCGATGGAGTACA+CGG | - | chr2.3:36087489-36087508 | None:intergenic | 50.0% | |
| GCTAAAAGCTCTTATGCCCG+TGG | + | chr2.3:36087463-36087482 | MS.gene067363:CDS | 50.0% | |
| GGATACTCCATGACGAACCA+AGG | - | chr2.3:36087761-36087780 | None:intergenic | 50.0% | |
| TACGAGGATGTAAGCAAGGG+AGG | - | chr2.3:36087300-36087319 | None:intergenic | 50.0% | |
| ! | GTCGTTTCCTTGGTTCGTCA+TGG | + | chr2.3:36087751-36087770 | MS.gene067363:CDS | 50.0% |
| !! | CAGGAAGAAGATTCTGAGGC+TGG | + | chr2.3:36088085-36088104 | MS.gene067363:CDS | 50.0% |
| !! | GGTGTCGTTGTAGCTGCTTA+TGG | + | chr2.3:36087566-36087585 | MS.gene067363:CDS | 50.0% |
| GGGAGGAACAAAAGGCCATG+TGG | - | chr2.3:36087283-36087302 | None:intergenic | 55.0% | |
| GGTGGCACTTAAGGCTTCTG+AGG | + | chr2.3:36088042-36088061 | MS.gene067363:CDS | 55.0% | |
| ! | AGCGTTTGAAGCCACGAGGT+TGG | + | chr2.3:36087637-36087656 | MS.gene067363:CDS | 55.0% |
| ! | CTGTAGCGTTTGAAGCCACG+AGG | + | chr2.3:36087633-36087652 | MS.gene067363:CDS | 55.0% |
| !! | TGGTGTTACGCTGCAGCTCA+TGG | + | chr2.3:36087610-36087629 | MS.gene067363:CDS | 55.0% |
| CCCGATGGAGTACACGGCCA+CGG | - | chr2.3:36087483-36087502 | None:intergenic | 65.0% | |
| CCCGTGGCCGTGTACTCCAT+CGG | + | chr2.3:36087479-36087498 | MS.gene067363:CDS | 65.0% | |
| CCGATGGAGTACACGGCCAC+GGG | - | chr2.3:36087482-36087501 | None:intergenic | 65.0% | |
| CCGTGGCCGTGTACTCCATC+GGG | + | chr2.3:36087480-36087499 | MS.gene067363:CDS | 65.0% | |
| CGTGGCCGTGTACTCCATCG+GGG | + | chr2.3:36087481-36087500 | MS.gene067363:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 36087125 | 36088177 | 36087125 | ID=MS.gene067363 |
| chr2.3 | mRNA | 36087125 | 36088177 | 36087125 | ID=MS.gene067363.t1;Parent=MS.gene067363 |
| chr2.3 | exon | 36087125 | 36088177 | 36087125 | ID=MS.gene067363.t1.exon1;Parent=MS.gene067363.t1 |
| chr2.3 | CDS | 36087125 | 36088177 | 36087125 | ID=cds.MS.gene067363.t1;Parent=MS.gene067363.t1 |
| Gene Sequence |
| Protein sequence |
