AlfalfaGEDB | MS.gene65609

M. sativa cultivar XinJiangDaYe / MS.gene65609

KEGG: K00128
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016620 GO:0016903 GO:0055114
PFAM: PF00171.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65609.t1	XP_024627803.1	98.5	541	7	1	1	540	1	541	9.8e-309	1068.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65609.t1	Q8S528	78.6	538	110	2	3	540	2	534	6.8e-250	864.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65609.t1	A0A072U2G7	98.5	541	7	1	1	540	1	541	7.1e-309	1068.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050170	MS.gene65609	-0.834845	2.37E-56	-1.69E-46
MS.gene050496	MS.gene65609	-0.832821	7.59E-56	-1.69E-46
MS.gene051963	MS.gene65609	0.813806	2.12E-51	-1.69E-46
MS.gene053083	MS.gene65609	0.860026	2.75E-63	-1.69E-46
MS.gene053826	MS.gene65609	0.80247	5.53E-49	-1.69E-46
MS.gene05726	MS.gene65609	0.820692	6.00E-53	-1.69E-46
MS.gene057499	MS.gene65609	0.815205	1.04E-51	-1.69E-46
MS.gene058996	MS.gene65609	0.822856	1.89E-53	-1.69E-46
MS.gene059837	MS.gene65609	-0.857264	1.84E-62	-1.69E-46
MS.gene060187	MS.gene65609	0.813313	2.73E-51	-1.69E-46
MS.gene060593	MS.gene65609	0.80116	1.03E-48	-1.69E-46
MS.gene060595	MS.gene65609	0.838061	3.60E-57	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene61417	MS.gene65609	PPI
MS.gene65609	MS.gene015742	PPI
MS.gene65609	MS.gene070228	PPI
MS.gene65609	MS.gene41133	PPI
MS.gene65609	MS.gene30561	PPI
MS.gene65609	MS.gene045715	PPI
MS.gene65609	MS.gene004003	PPI
MS.gene65609	MS.gene037163	PPI
MS.gene65609	MS.gene33139	PPI
MS.gene65609	MS.gene025244	PPI
MS.gene65609	MS.gene049130	PPI
MS.gene65609	MS.gene05924	PPI
MS.gene65609	MS.gene028502	PPI
MS.gene65609	MS.gene62486	PPI
MS.gene015744	MS.gene65609	PPI
MS.gene65609	MS.gene05926	PPI
MS.gene65609	MS.gene01226	PPI
MS.gene65609	MS.gene042955	PPI
MS.gene65609	MS.gene00160	PPI
MS.gene65609	MS.gene55995	PPI
MS.gene62087	MS.gene65609	PPI
MS.gene65609	MS.gene61421	PPI
MS.gene65609	MS.gene042286	PPI
MS.gene65609	MS.gene012582	PPI
MS.gene65609	MS.gene057392	PPI
MS.gene65609	MS.gene033206	PPI
MS.gene65609	MS.gene052440	PPI
MS.gene65609	MS.gene058231	PPI
MS.gene65609	MS.gene05526	PPI
MS.gene042956	MS.gene65609	PPI
MS.gene65609	MS.gene021154	PPI
MS.gene05525	MS.gene65609	PPI
MS.gene65609	MS.gene052441	PPI
MS.gene038961	MS.gene65609	PPI
MS.gene65609	MS.gene068009	PPI
MS.gene65609	MS.gene035576	PPI
MS.gene65609	MS.gene43277	PPI
MS.gene65609	MS.gene053596	PPI
MS.gene65609	MS.gene60330	PPI
MS.gene65609	MS.gene90245	PPI
MS.gene65609	MS.gene002253	PPI
MS.gene65609	MS.gene64463	PPI
MS.gene65609	MS.gene016051	PPI
MS.gene56963	MS.gene65609	PPI
MS.gene65609	MS.gene04769	PPI
MS.gene90238	MS.gene65609	PPI
MS.gene65609	MS.gene066203	PPI
MS.gene65609	MS.gene04770	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65609.t1	MTR_8g064170	98.521	541	7	1	1	540	1	541	0.0	1099
MS.gene65609.t1	MTR_8g064170	92.606	541	7	2	1	540	1	509	0.0	1018
MS.gene65609.t1	MTR_3g078170	75.506	543	120	2	10	540	1	542	0.0	876
MS.gene65609.t1	MTR_4g107040	80.517	503	97	1	38	540	43	544	0.0	872
MS.gene65609.t1	MTR_3g078170	73.626	455	107	2	10	452	1	454	0.0	717
MS.gene65609.t1	MTR_3g078170	73.380	432	102	2	10	429	1	431	0.0	681
MS.gene65609.t1	MTR_3g078170	73.380	432	102	2	10	429	1	431	0.0	681
MS.gene65609.t1	MTR_6g086300	57.851	484	204	0	57	540	19	502	0.0	578
MS.gene65609.t1	MTR_6g086300	57.851	484	204	0	57	540	19	502	0.0	578
MS.gene65609.t1	MTR_8g083530	54.339	484	221	0	57	540	20	503	0.0	562
MS.gene65609.t1	MTR_6g086340	55.579	484	215	0	57	540	17	500	0.0	552
MS.gene65609.t1	MTR_8g083520	51.324	491	206	1	50	540	4	461	0.0	521
MS.gene65609.t1	MTR_8g083520	50.242	414	173	1	127	540	1	381	2.99e-147	428
MS.gene65609.t1	MTR_8g083530	53.333	330	154	0	57	386	20	349	4.53e-124	369
MS.gene65609.t1	MTR_3g078550	39.148	493	287	6	57	539	5	494	3.06e-115	350
MS.gene65609.t1	MTR_4g106510	40.606	495	281	6	55	539	3	494	1.85e-113	346
MS.gene65609.t1	MTR_3g078550	40.618	453	256	6	97	539	15	464	1.12e-109	335
MS.gene65609.t1	MTR_3g078490	38.353	498	290	6	54	539	32	524	2.56e-109	337
MS.gene65609.t1	MTR_2g067660	38.554	498	298	5	37	530	21	514	1.09e-103	322
MS.gene65609.t1	MTR_1690s0010	44.575	341	184	4	61	399	10	347	3.07e-83	263
MS.gene65609.t1	MTR_3g078490	36.735	343	206	5	54	388	2	341	9.34e-63	210
MS.gene65609.t1	MTR_1g014320	31.762	488	305	10	65	539	21	493	2.02e-61	210
MS.gene65609.t1	MTR_2g034160	30.273	512	318	11	50	531	26	528	1.47e-60	210
MS.gene65609.t1	MTR_4g131180	31.148	488	308	10	65	539	21	493	4.55e-59	204
MS.gene65609.t1	MTR_2g042330	30.655	473	309	11	66	526	23	488	3.46e-58	202
MS.gene65609.t1	MTR_4g131180	31.579	437	280	8	65	493	21	446	3.83e-55	193
MS.gene65609.t1	MTR_2g009750	31.276	486	312	11	63	536	18	493	2.42e-54	191
MS.gene65609.t1	MTR_2g009750	31.134	485	313	11	63	536	18	492	1.79e-53	189
MS.gene65609.t1	MTR_2g009750	31.134	485	313	11	63	536	17	491	1.81e-53	189
MS.gene65609.t1	MTR_4g093170	31.200	500	307	13	58	536	37	520	6.49e-53	188
MS.gene65609.t1	MTR_1399s0020	28.650	363	207	5	168	528	23	335	1.35e-39	147
MS.gene65609.t1	MTR_8g076480	29.047	451	284	14	98	531	8	439	2.38e-36	141
MS.gene65609.t1	MTR_1222s0010	32.687	361	214	11	129	476	33	377	8.03e-35	139
MS.gene65609.t1	MTR_1g029840	28.868	433	290	11	107	531	24	446	1.18e-33	134
MS.gene65609.t1	MTR_1g029840	28.079	406	278	9	133	531	48	446	1.58e-31	127
MS.gene65609.t1	MTR_8g094600	27.235	481	326	14	61	531	33	499	2.18e-30	125
MS.gene65609.t1	MTR_2g097950	29.103	457	287	16	93	530	14	452	8.61e-30	123
MS.gene65609.t1	MTR_3g088150	27.169	438	299	13	103	531	22	448	2.01e-29	121
MS.gene65609.t1	MTR_1314s0010	29.480	346	222	7	197	530	1	336	2.95e-28	115
MS.gene65609.t1	MTR_2g097950	26.304	460	279	14	85	530	4	417	1.81e-24	107
MS.gene65609.t1	MTR_4g065040	28.469	418	263	15	133	531	38	438	1.73e-20	95.1
MS.gene65609.t1	MTR_0002s0820	46.667	120	47	2	240	342	29	148	2.18e-20	88.6
MS.gene65609.t1	MTR_4g107940	25.304	411	270	15	63	450	54	450	1.30e-11	67.4
MS.gene65609.t1	MTR_4g107940	25.304	411	270	15	63	450	54	450	1.46e-11	67.0
MS.gene65609.t1	MTR_8g064020	89.286	28	3	0	513	540	53	80	3.75e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65609.t1	AT3G48000	77.196	535	121	1	6	540	5	538	0.0	882
MS.gene65609.t1	AT1G23800	81.474	502	93	0	39	540	33	534	0.0	874
MS.gene65609.t1	AT1G23800	82.370	346	61	0	39	384	33	378	0.0	615
MS.gene65609.t1	AT3G24503	55.556	486	215	1	56	540	16	501	0.0	559
MS.gene65609.t1	AT3G48170	41.734	496	276	6	54	539	2	494	2.61e-129	387
MS.gene65609.t1	AT1G74920	41.104	489	275	6	61	539	9	494	4.05e-123	371
MS.gene65609.t1	AT1G74920	41.309	489	269	7	61	539	9	489	1.93e-121	366
MS.gene65609.t1	AT1G79440	40.169	473	278	4	59	530	52	520	2.08e-104	323
MS.gene65609.t1	AT2G24270	31.911	492	299	11	65	539	21	493	1.10e-58	203
MS.gene65609.t1	AT2G24270	31.911	492	299	11	65	539	21	493	1.10e-58	203
MS.gene65609.t1	AT2G24270	31.911	492	299	11	65	539	21	493	1.82e-58	202
MS.gene65609.t1	AT3G66658	28.684	509	330	10	50	531	26	528	1.92e-58	204
MS.gene65609.t1	AT1G54100	31.049	467	300	12	72	526	31	487	3.54e-58	202
MS.gene65609.t1	AT1G54100	31.049	467	300	12	72	526	31	487	3.54e-58	202
MS.gene65609.t1	AT2G24270	31.911	492	299	11	65	539	61	533	4.92e-58	202
MS.gene65609.t1	AT3G66658	28.684	509	330	10	50	531	26	528	6.19e-58	203
MS.gene65609.t1	AT2G14170	33.030	439	277	10	63	493	8	437	2.62e-57	199
MS.gene65609.t1	AT2G14170	33.030	439	277	10	63	493	117	546	1.16e-56	200
MS.gene65609.t1	AT2G14170	31.417	487	310	12	63	536	117	592	1.73e-56	199
MS.gene65609.t1	AT1G44170	29.054	444	297	12	96	531	9	442	3.55e-39	149
MS.gene65609.t1	AT1G44170	29.054	444	297	12	96	531	9	442	3.55e-39	149
MS.gene65609.t1	AT1G44170	30.259	347	232	8	188	531	40	379	2.33e-34	134
MS.gene65609.t1	AT4G36250	27.912	455	297	16	93	531	2	441	4.54e-32	129
MS.gene65609.t1	AT4G34240	28.736	348	236	8	188	531	24	363	3.65e-28	117
MS.gene65609.t1	AT4G34240	28.736	348	236	8	188	531	166	505	2.46e-27	116
MS.gene65609.t1	AT4G34240	28.736	348	236	8	188	531	151	490	2.94e-27	115
MS.gene65609.t1	AT4G34240	38.065	155	91	4	188	341	166	316	4.94e-15	77.4

Find 120 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCAGGTGGGAATGGTTATC+TGG	0.219323	8.2:-37648890	MS.gene65609:intron
AGTGGACAGGGCAGAGAAAA+AGG	0.274646	8.2:-37646004	MS.gene65609:CDS
CACGAACCAATTGGCGTTGC+TGG	0.282957	8.2:-37648039	MS.gene65609:CDS
GTCTCACTTGAATTTCAATA+TGG	0.295168	8.2:+37646478	None:intergenic
TTTACTGGTTCAACTGATAC+TGG	0.300808	8.2:-37647407	MS.gene65609:CDS
TCTTCTCATGTTTGCTTGGA+AGG	0.308492	8.2:-37647992	MS.gene65609:CDS
CTCATGTTTGCTTGGAAGGT+TGG	0.312209	8.2:-37647988	MS.gene65609:CDS
GCACTTGAGACTTGGGATAA+TGG	0.315242	8.2:-37648283	MS.gene65609:CDS
CCACCAAGCTCCAAAGTTAC+CGG	0.326290	8.2:+37647344	None:intergenic
TTTGATGCTGCAATTCCTTT+TGG	0.326962	8.2:-37646040	MS.gene65609:CDS
ATCTGAGCAGATTGTTCATA+TGG	0.331515	8.2:+37648256	None:intergenic
ATTGCAAAAGAAGAGATATT+TGG	0.335473	8.2:-37646512	MS.gene65609:CDS
ACATACCTGGTTGAAGAATA+AGG	0.339394	8.2:+37647256	None:intergenic
CACTTGAGACTTGGGATAAT+GGG	0.341726	8.2:-37648282	MS.gene65609:CDS
CATTGAAAGTGGGAACAGTT+TGG	0.350522	8.2:-37646081	MS.gene65609:CDS
TACTGTCTTCTCAAATGTTC+AGG	0.364449	8.2:-37646651	MS.gene65609:intron
CTACAAATTTGCCATCGATT+AGG	0.366038	8.2:+37648782	None:intergenic
CAATCTTAAGCCGGTAACTT+TGG	0.372001	8.2:-37647354	MS.gene65609:CDS
ACTTGCACCGATGGTTTAAT+CGG	0.379104	8.2:+37648820	None:intergenic
ATTAGATCTGGCGTTGATAA+TGG	0.379386	8.2:-37646731	MS.gene65609:CDS
ATTGAAAGTGGGAACAGTTT+GGG	0.385160	8.2:-37646080	MS.gene65609:CDS
AGAGCAAACAATTCAAAGTA+TGG	0.393602	8.2:-37646160	MS.gene65609:CDS
TCCCTCTTCTCATGTTTGCT+TGG	0.401686	8.2:-37647996	MS.gene65609:CDS
TTGAACATAATATCTGGCTT+TGG	0.404964	8.2:-37647731	MS.gene65609:CDS
CAGGGACAATGTTGCTGTGC+CGG	0.407196	8.2:-37647154	MS.gene65609:CDS
AATTTCAATATGGTTTGTAC+TGG	0.414009	8.2:+37646488	None:intergenic
TGTTCTGTTCCTGACTTGAA+TGG	0.414781	8.2:+37647046	None:intergenic
TTGTAGGCTGGACTTCCTCC+TGG	0.416956	8.2:-37647758	MS.gene65609:intron
TGCAATGTCTGCACATGATA+TGG	0.425470	8.2:+37648060	None:intergenic
TACCAGCATAGTAACGAATT+AGG	0.432343	8.2:+37648212	None:intergenic
AGCAATGATCATACATAAGC+TGG	0.439518	8.2:+37648484	None:intergenic
ATCTGACCAGCAACGCCAAT+TGG	0.441277	8.2:+37648033	None:intergenic
GCATCTTCGCAAACAATAAA+CGG	0.445334	8.2:+37647314	None:intergenic
CATACATAAGCTGGAAACTT+AGG	0.447847	8.2:+37648493	None:intergenic
CAGACATTGCACGAACCAAT+TGG	0.452449	8.2:-37648048	MS.gene65609:CDS
AAGCCGGTAACTTTGGAGCT+TGG	0.455012	8.2:-37647347	MS.gene65609:CDS
AATGGCGCTACACTTGAAAC+TGG	0.456541	8.2:-37646713	MS.gene65609:CDS
CGCCTAATTCGTTACTATGC+TGG	0.456816	8.2:-37648214	MS.gene65609:intron
CGAATTAGGCGAACCAGCAT+TGG	0.458512	8.2:+37648226	None:intergenic
AAATTGCAGCACTTGAGACT+TGG	0.458601	8.2:-37648291	MS.gene65609:CDS
GCAATCACTTCCCCTGTCCT+TGG	0.470122	8.2:+37648595	None:intergenic
GCACGCAAAGCATTTGATCA+AGG	0.473740	8.2:-37648523	MS.gene65609:CDS
GGTTGGGCAGATAAGATTCA+TGG	0.473931	8.2:-37648105	MS.gene65609:CDS
TGCTTTGCGTGCAGCTGCAA+CGG	0.475234	8.2:+37648534	None:intergenic
TGGAAAAGTTGTCCTTCAAC+TGG	0.479571	8.2:-37647387	MS.gene65609:CDS
CCGGTAACTTTGGAGCTTGG+TGG	0.486152	8.2:-37647344	MS.gene65609:CDS
AATGTACTTTCAGGTGGGAA+TGG	0.487239	8.2:-37648898	MS.gene65609:intron
AGGGACAATGTTGCTGTGCC+GGG	0.490364	8.2:-37647153	MS.gene65609:CDS
TCAAAGTATGGTCTTGCAGC+AGG	0.491649	8.2:-37646148	MS.gene65609:CDS
TAGTCACATGGATGTTGATA+AGG	0.496076	8.2:-37647684	MS.gene65609:intron
TCAGATTGAAATTCCAATGC+TGG	0.496455	8.2:-37648239	MS.gene65609:CDS
TCTGGCTTTGGTCCAACTGC+AGG	0.500521	8.2:-37647719	MS.gene65609:CDS
CCAACATTGGATCCAAGGAC+AGG	0.507885	8.2:-37648607	MS.gene65609:CDS
CCTGTCCTTGGATCCAATGT+TGG	0.511997	8.2:+37648607	None:intergenic
CTCATCTTATATCCACCAAA+AGG	0.515568	8.2:+37646025	None:intergenic
ATATTGAAGTATATTAGATC+TGG	0.517435	8.2:-37646743	MS.gene65609:CDS
CGGTAACTTTGGAGCTTGGT+GGG	0.520556	8.2:-37647343	MS.gene65609:CDS
CTTCTTCAATCAAAATCTCA+AGG	0.522051	8.2:-37649772	MS.gene65609:CDS
GGGCTCACAGTTCCAGCTGA+TGG	0.526400	8.2:-37648084	MS.gene65609:CDS
TTGGAAGGTTGGACCTGCAT+TGG	0.527909	8.2:-37647977	MS.gene65609:CDS
GTAGGAGATCCATTCAAGTC+AGG	0.532267	8.2:-37647055	MS.gene65609:CDS
TCAGGTGGGAATGGTTATCT+GGG	0.532469	8.2:-37648889	MS.gene65609:intron
AATTGCAGCACTTGAGACTT+GGG	0.535187	8.2:-37648290	MS.gene65609:CDS
AGGTAAAACATTTCCAACAT+TGG	0.536576	8.2:-37648620	MS.gene65609:CDS
TGGATATAAGATGAGTGGAC+AGG	0.537139	8.2:-37646017	MS.gene65609:CDS
CTTTGCCTTATTCTTCAACC+AGG	0.537670	8.2:-37647261	MS.gene65609:intron
AGGAACAGAACAAGGTCCTC+AGG	0.538549	8.2:-37647035	MS.gene65609:intron
ACAGATGCTCAAAGCCATGC+TGG	0.538877	8.2:+37645923	None:intergenic
ATGAGAAGAGGGAAGTTCCA+TGG	0.539559	8.2:+37648006	None:intergenic
GTGTTGCCACACGCCAATGC+AGG	0.541029	8.2:+37647964	None:intergenic
CATTTCCAACATTGGATCCA+AGG	0.543139	8.2:-37648612	MS.gene65609:CDS
ACATGATATGGTCCATCAGC+TGG	0.548696	8.2:+37648072	None:intergenic
GTGTGGTCGACTTGCACCGA+TGG	0.550868	8.2:+37648811	None:intergenic
GCAAGAGCAGCACCTGCAGT+TGG	0.553046	8.2:+37647707	None:intergenic
TGCTGCTCTTGCTAGTCACA+TGG	0.554143	8.2:-37647696	MS.gene65609:CDS
AGCTAAAAGCAATCTTAAGC+CGG	0.554195	8.2:-37647363	MS.gene65609:CDS
CACGCAAAGCATTTGATCAA+GGG	0.557713	8.2:-37648522	MS.gene65609:CDS
TACAGCACGTTTCAAAGCAC+GGG	0.560601	8.2:+37647075	None:intergenic
CAACATTGGATCCAAGGACA+GGG	0.563764	8.2:-37648606	MS.gene65609:CDS
AAGCATTTGATCAAGGGCCG+TGG	0.572236	8.2:-37648516	MS.gene65609:CDS
AAATTTGTAGATGCTGCTGC+TGG	0.577592	8.2:-37648769	MS.gene65609:intron
TTGATTGGTTCAAGGGACCT+TGG	0.579537	8.2:-37646196	MS.gene65609:intron
TAATTTACCGCGAGAAAACA+AGG	0.580941	8.2:+37649709	None:intergenic
CTACAGCACGTTTCAAAGCA+CGG	0.582648	8.2:+37647074	None:intergenic
TCCAAGCAAACATGAGAAGA+GGG	0.585819	8.2:+37647995	None:intergenic
AGGAGAAAGACTTGGTAGCA+AGG	0.587482	8.2:-37646690	MS.gene65609:CDS
ACCGCGAGAAAACAAGGAGG+TGG	0.588337	8.2:+37649715	None:intergenic
ATGTACAAATGTTCGAGACC+CGG	0.588405	8.2:+37647135	None:intergenic
AGTGATTGCTCATGTTGCTG+AGG	0.588599	8.2:-37648581	MS.gene65609:CDS
TTTGGTGGATATAAGATGAG+TGG	0.589571	8.2:-37646022	MS.gene65609:CDS
CTCTTTGAATCACCTCACCA+AGG	0.589796	8.2:+37646179	None:intergenic
TTCAAGTCAGGAACAGAACA+AGG	0.593415	8.2:-37647043	MS.gene65609:CDS
GGATATAAGATGAGTGGACA+GGG	0.593641	8.2:-37646016	MS.gene65609:CDS
CACCTTTGAAGAATCCAGCA+TGG	0.596410	8.2:-37645937	MS.gene65609:CDS
GTTGGGCAGATAAGATTCAT+GGG	0.597943	8.2:-37648104	MS.gene65609:CDS
TTCCAAGCAAACATGAGAAG+AGG	0.599102	8.2:+37647994	None:intergenic
GGAGAAAGACTTGGTAGCAA+GGG	0.599344	8.2:-37646689	MS.gene65609:CDS
CGTGCTTTGAAACGTGCTGT+AGG	0.601175	8.2:-37647073	MS.gene65609:CDS
TTTGACTAGAGCATTGAAAG+TGG	0.602275	8.2:-37646092	MS.gene65609:CDS
TTGACTAGAGCATTGAAAGT+GGG	0.603811	8.2:-37646091	MS.gene65609:CDS
GGTGGGAATGGTTATCTGGG+TGG	0.605677	8.2:-37648886	MS.gene65609:CDS
AGCCATGCTGGATTCTTCAA+AGG	0.612311	8.2:+37645935	None:intergenic
TTGCTGGTCAGATCATTCCA+TGG	0.623473	8.2:-37648023	MS.gene65609:CDS
GAAACTGGAGGAGAAAGACT+TGG	0.623804	8.2:-37646698	MS.gene65609:CDS
CACACTCAGCTCCTAATCGA+TGG	0.626153	8.2:-37648793	MS.gene65609:CDS
TGAACATTTGAGAAGACAGT+AGG	0.631599	8.2:+37646653	None:intergenic
GCTACATACAAAGCAGACAA+TGG	0.633191	8.2:+37647913	None:intergenic
GATATTATGTTCAAAACACC+AGG	0.634494	8.2:+37647740	None:intergenic
GTGATTGCTCATGTTGCTGA+GGG	0.636914	8.2:-37648580	MS.gene65609:CDS
GGCGCTACACTTGAAACTGG+AGG	0.646681	8.2:-37646710	MS.gene65609:CDS
GTTGGACCTGCATTGGCGTG+TGG	0.651240	8.2:-37647970	MS.gene65609:CDS
TAAGCTGGAAACTTAGGCCA+CGG	0.652576	8.2:+37648499	None:intergenic
TGGTTCAAGGGACCTTGGTG+AGG	0.655055	8.2:-37646191	MS.gene65609:intron
GGAGTTTGTTGAGAAAGCAA+AGG	0.658022	8.2:-37647098	MS.gene65609:CDS
TGTAGCAAAACTATTTCACG+AGG	0.677900	8.2:-37647896	MS.gene65609:intron
TGTACATGAACGTGTGCATG+AGG	0.681956	8.2:-37647119	MS.gene65609:CDS
AACATTGGATCCAAGGACAG+GGG	0.683175	8.2:-37648605	MS.gene65609:CDS
CATCGATTAGGAGCTGAGTG+TGG	0.695634	8.2:+37648794	None:intergenic
ATTATGTTCAAAACACCAGG+AGG	0.713074	8.2:+37647743	None:intergenic
TTTACCGCGAGAAAACAAGG+AGG	0.831635	8.2:+37649712	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATAAAAATTAAAACAAGAT+TGG	+	chr8.2:37647850-37647869	None:intergenic	10.0%
!!	ATGATAAATTGAATATTGAA+AGG	-	chr8.2:37646778-37646797	MS.gene65609:intron	15.0%
!!!	AATAAGATAAGATAAGATTT+TGG	-	chr8.2:37646217-37646236	MS.gene65609:intron	15.0%
!!!	AGTTTTTAGAAAGAAATATT+CGG	+	chr8.2:37646639-37646658	None:intergenic	15.0%
!!!	CAATTAATTTTAATGTTGAT+TGG	-	chr8.2:37649496-37649515	MS.gene65609:intron	15.0%
!!!	CTTTTTATACAATTTTGATT+AGG	-	chr8.2:37646988-37647007	MS.gene65609:intron	15.0%
!!!	TCAGTTTATAATTTTCATTA+GGG	-	chr8.2:37646721-37646740	MS.gene65609:CDS	15.0%
!!!	TTCAGTTTATAATTTTCATT+AGG	-	chr8.2:37646720-37646739	MS.gene65609:CDS	15.0%
!!	ATATTGAAGTATATTAGATC+TGG	-	chr8.2:37648964-37648983	MS.gene65609:intron	20.0%
!!	CAGATACAATTAAAAACATT+AGG	+	chr8.2:37649149-37649168	None:intergenic	20.0%
!!	GAAAAAAAAAATGTACTTTC+AGG	-	chr8.2:37646800-37646819	MS.gene65609:intron	20.0%
!!	GAAATGCATGTTTAAATTAA+CGG	-	chr8.2:37646499-37646518	MS.gene65609:CDS	20.0%
!!	TAATTTAAACATGCATTTCA+AGG	+	chr8.2:37646498-37646517	None:intergenic	20.0%
!!!	AGCAAAGATGATATATTTTA+TGG	+	chr8.2:37647914-37647933	None:intergenic	20.0%
!!!	CAATATAAAAGCATTGTTTT+TGG	-	chr8.2:37647873-37647892	MS.gene65609:intron	20.0%
!!!	CAGTTTATAATTTTCATTAG+GGG	-	chr8.2:37646722-37646741	MS.gene65609:CDS	20.0%
!!!	TAATTTGTAATCTTTCTTGT+AGG	-	chr8.2:37647933-37647952	MS.gene65609:CDS	20.0%
!!!	TCTCTAGAAAATTTTATAAG+AGG	-	chr8.2:37649420-37649439	MS.gene65609:intron	20.0%
!!!	TTCTAAGAAAATGATTTTCT+GGG	-	chr8.2:37646137-37646156	MS.gene65609:CDS	20.0%
!!!	TTTCTAAGAAAATGATTTTC+TGG	-	chr8.2:37646136-37646155	MS.gene65609:CDS	20.0%
!!!	TTTTGAATTTGATTGATGAT+AGG	-	chr8.2:37646164-37646183	MS.gene65609:CDS	20.0%
!!!	TTTTTCTTTCATTAATTGCA+TGG	-	chr8.2:37646283-37646302	MS.gene65609:intron	20.0%
!	AAAAAAAATGTACTTTCAGG+TGG	-	chr8.2:37646803-37646822	MS.gene65609:intron	25.0%
!	AAGAAAAGAGGATTAACTAA+AGG	-	chr8.2:37647012-37647031	MS.gene65609:intron	25.0%
!	ATATAGACTACATTTGATGA+AGG	+	chr8.2:37649324-37649343	None:intergenic	25.0%
!	ATTGCAAAAGAAGAGATATT+TGG	-	chr8.2:37649195-37649214	MS.gene65609:intron	25.0%
!	ATTGCATTGCACATAAAAAT+AGG	+	chr8.2:37649480-37649499	None:intergenic	25.0%
!	CATATTACTATAGCCAAAAA+AGG	+	chr8.2:37646417-37646436	None:intergenic	25.0%
!	GATGTTATCTTGATTTCAAT+TGG	-	chr8.2:37646681-37646700	MS.gene65609:CDS	25.0%
!	GATTAACTAAAGGAAAAGAA+GGG	-	chr8.2:37647022-37647041	MS.gene65609:intron	25.0%
!	GGCTATAGTAATATGAAAAT+TGG	-	chr8.2:37646422-37646441	MS.gene65609:intron	25.0%
!	GTTTGTCAAGTCATTATATT+TGG	-	chr8.2:37647277-37647296	MS.gene65609:CDS	25.0%
!	TATAGACTACATTTGATGAA+GGG	+	chr8.2:37649323-37649342	None:intergenic	25.0%
!	TTATATTCTCATCTTTGCAA+AGG	-	chr8.2:37649106-37649125	MS.gene65609:intron	25.0%
!	TTGCATTGCACATAAAAATA+GGG	+	chr8.2:37649479-37649498	None:intergenic	25.0%
!	TTTGAATCAATCTGATTGTT+TGG	+	chr8.2:37648931-37648950	None:intergenic	25.0%
!	TTTGCAAAAATCGTCAAATT+GGG	+	chr8.2:37649370-37649389	None:intergenic	25.0%
!	TTTGTACATGTTTAGATTGA+TGG	-	chr8.2:37646445-37646464	MS.gene65609:intron	25.0%
!!	AAAAAAATGTACTTTCAGGT+GGG	-	chr8.2:37646804-37646823	MS.gene65609:intron	25.0%
!!	AATTTCAATATGGTTTGTAC+TGG	+	chr8.2:37649222-37649241	None:intergenic	25.0%
!!	ATAATTTTCATTAGGGGTTT+AGG	-	chr8.2:37646728-37646747	MS.gene65609:CDS	25.0%
!!	ATTTTTGTTAAGTCCATCTT+TGG	+	chr8.2:37646578-37646597	None:intergenic	25.0%
!!	TCTAAGAAAATGATTTTCTG+GGG	-	chr8.2:37646138-37646157	MS.gene65609:CDS	25.0%
!!	TTTAATGTTGATTGGTTCAA+GGG	-	chr8.2:37649504-37649523	MS.gene65609:intron	25.0%
!!	TTTCAATTGGTGATTAAAAG+TGG	-	chr8.2:37646694-37646713	MS.gene65609:CDS	25.0%
!!	TTTTGCAAAAATCGTCAAAT+TGG	+	chr8.2:37649371-37649390	None:intergenic	25.0%
!!!	ATAAATCAAATAGCACTGAT+TGG	-	chr8.2:37648514-37648533	MS.gene65609:CDS	25.0%
!!!	TATAAAAGCATTGTTTTTGG+TGG	-	chr8.2:37647876-37647895	MS.gene65609:intron	25.0%
!!!	TATAAGAGGTGTATTTTACA+AGG	-	chr8.2:37649434-37649453	MS.gene65609:intron	25.0%
!!!	TTTAATCGGTTTTTCATCAA+TGG	+	chr8.2:37646876-37646895	None:intergenic	25.0%
!!!	TTTCTGTTTCTGTGTTTTAT+AGG	-	chr8.2:37647339-37647358	MS.gene65609:CDS	25.0%
!!!	TTTTAATGTTGATTGGTTCA+AGG	-	chr8.2:37649503-37649522	MS.gene65609:intron	25.0%
!!!	TTTTGATTAGGAAAGAAAAG+AGG	-	chr8.2:37647000-37647019	MS.gene65609:intron	25.0%
	ACTTTATGATTGAACATACC+TGG	+	chr8.2:37648467-37648486	None:intergenic	30.0%
	AGAGCAAACAATTCAAAGTA+TGG	-	chr8.2:37649547-37649566	MS.gene65609:intron	30.0%
	AGGTAAAACATTTCCAACAT+TGG	-	chr8.2:37647087-37647106	MS.gene65609:CDS	30.0%
	CAAAAGAGTCACAAACATTA+AGG	+	chr8.2:37648872-37648891	None:intergenic	30.0%
	CAGAAACAGAAAAACAAAAG+TGG	+	chr8.2:37647331-37647350	None:intergenic	30.0%
	CTTCTTCAATCAAAATCTCA+AGG	-	chr8.2:37645935-37645954	MS.gene65609:CDS	30.0%
	GATATTATGTTCAAAACACC+AGG	+	chr8.2:37647970-37647989	None:intergenic	30.0%
	GGATTAACTAAAGGAAAAGA+AGG	-	chr8.2:37647021-37647040	MS.gene65609:intron	30.0%
	GTCTCACTTGAATTTCAATA+TGG	+	chr8.2:37649232-37649251	None:intergenic	30.0%
	GTTCAACTTCAATGAATTTG+CGG	-	chr8.2:37647299-37647318	MS.gene65609:CDS	30.0%
	TAAAAACTACTTGCAAGTGA+AGG	-	chr8.2:37649738-37649757	MS.gene65609:CDS	30.0%
	TATTCGTTGTAATTTACCTG+AGG	+	chr8.2:37648691-37648710	None:intergenic	30.0%
	TCATGATTAATCTTAGAGCT+TGG	-	chr8.2:37648726-37648745	MS.gene65609:intron	30.0%
	TCTTTCATTAATTGCATGGT+TGG	-	chr8.2:37646287-37646306	MS.gene65609:intron	30.0%
	TGAAAAACCGATTAAACCAT+CGG	-	chr8.2:37646880-37646899	MS.gene65609:intron	30.0%
	TTTAAACATGCATTTCAAGG+TGG	+	chr8.2:37646495-37646514	None:intergenic	30.0%
!	ATGCCTTCAATCTAAAATGT+CGG	+	chr8.2:37648130-37648149	None:intergenic	30.0%
!	GTTTAGATTGATGGTCTATT+TGG	-	chr8.2:37646454-37646473	MS.gene65609:intron	30.0%
!	GTTTCTGTGTTTTATAGGAA+AGG	-	chr8.2:37647344-37647363	MS.gene65609:CDS	30.0%
!	TGTATTTTCATATTTACCCC+AGG	-	chr8.2:37647581-37647600	MS.gene65609:intron	30.0%
!	TTGAACATAATATCTGGCTT+TGG	-	chr8.2:37647976-37647995	MS.gene65609:CDS	30.0%
!	TTTTGTGGTATCTTACCTTT+TGG	-	chr8.2:37647049-37647068	MS.gene65609:CDS	30.0%
!!	GGTGTTTTGAACATAATATC+TGG	-	chr8.2:37647970-37647989	MS.gene65609:CDS	30.0%
!!	TTGGTTTTCCTAATTCTCTA+CGG	-	chr8.2:37648745-37648764	MS.gene65609:intron	30.0%
!!!	TAGGGGTTTAGGTTTTTTAA+AGG	-	chr8.2:37646739-37646758	MS.gene65609:CDS	30.0%
	ACATACCTGGTTGAAGAATA+AGG	+	chr8.2:37648454-37648473	None:intergenic	35.0%
	AGCAATGATCATACATAAGC+TGG	+	chr8.2:37647226-37647245	None:intergenic	35.0%
	AGCTAAAAGCAATCTTAAGC+CGG	-	chr8.2:37648344-37648363	MS.gene65609:CDS	35.0%
	ATCTGAGCAGATTGTTCATA+TGG	+	chr8.2:37647454-37647473	None:intergenic	35.0%
	ATCTGTGTGACATGAAAGAA+AGG	-	chr8.2:37646256-37646275	MS.gene65609:intron	35.0%
	ATTATGTTCAAAACACCAGG+AGG	+	chr8.2:37647967-37647986	None:intergenic	35.0%
	ATTGAAAGTGGGAACAGTTT+GGG	-	chr8.2:37649627-37649646	MS.gene65609:intron	35.0%
	ATTGACACTAGATCCAAAGA+TGG	-	chr8.2:37646562-37646581	MS.gene65609:intron	35.0%
	CAAAAGATTGTCCAAGATTC+AGG	+	chr8.2:37648271-37648290	None:intergenic	35.0%
	CAATGAGATTGACGAAATCA+CGG	+	chr8.2:37646611-37646630	None:intergenic	35.0%
	CATACATAAGCTGGAAACTT+AGG	+	chr8.2:37647217-37647236	None:intergenic	35.0%
	CTCATCTTATATCCACCAAA+AGG	+	chr8.2:37649685-37649704	None:intergenic	35.0%
	CTCTACAAATCAAGACTCAT+TGG	+	chr8.2:37649254-37649273	None:intergenic	35.0%
	GCATCTTCGCAAACAATAAA+CGG	+	chr8.2:37648396-37648415	None:intergenic	35.0%
	TAATTTACCGCGAGAAAACA+AGG	+	chr8.2:37646001-37646020	None:intergenic	35.0%
	TACCAGCATAGTAACGAATT+AGG	+	chr8.2:37647498-37647517	None:intergenic	35.0%
	TACTGTCTTCTCAAATGTTC+AGG	-	chr8.2:37649056-37649075	MS.gene65609:intron	35.0%
	TAGTCACATGGATGTTGATA+AGG	-	chr8.2:37648023-37648042	MS.gene65609:CDS	35.0%
	TCAGATTGAAATTCCAATGC+TGG	-	chr8.2:37647468-37647487	MS.gene65609:intron	35.0%
	TGAACATTTGAGAAGACAGT+AGG	+	chr8.2:37649057-37649076	None:intergenic	35.0%
	TGTAGCAAAACTATTTCACG+AGG	-	chr8.2:37647811-37647830	MS.gene65609:intron	35.0%
	TGTATCTGTAACAATCATCC+AGG	-	chr8.2:37649161-37649180	MS.gene65609:intron	35.0%
	TTGTAATCTTTCTTGTAGGC+TGG	-	chr8.2:37647937-37647956	MS.gene65609:CDS	35.0%
	TTGTGATGTTCAGATACTAG+AGG	-	chr8.2:37648771-37648790	MS.gene65609:CDS	35.0%
	TTTCATATTTACCCCAGGTT+GGG	-	chr8.2:37647586-37647605	MS.gene65609:intron	35.0%
!	CAATCTAAAATGTCGGTACA+TGG	+	chr8.2:37648123-37648142	None:intergenic	35.0%
!	CTACAAATTTGCCATCGATT+AGG	+	chr8.2:37646928-37646947	None:intergenic	35.0%
!	TTGATGAAGGGTGCATTAAA+GGG	+	chr8.2:37649311-37649330	None:intergenic	35.0%
!	TTTGATGAAGGGTGCATTAA+AGG	+	chr8.2:37649312-37649331	None:intergenic	35.0%
!	TTTGATGCTGCAATTCCTTT+TGG	-	chr8.2:37649667-37649686	MS.gene65609:intron	35.0%
!	TTTGGTGGATATAAGATGAG+TGG	-	chr8.2:37649685-37649704	MS.gene65609:intron	35.0%
!	TTTTCATATTTACCCCAGGT+TGG	-	chr8.2:37647585-37647604	MS.gene65609:intron	35.0%
!!	ATTAGATCTGGCGTTGATAA+TGG	-	chr8.2:37648976-37648995	MS.gene65609:intron	35.0%
!!	GTACCGACATTTTAGATTGA+AGG	-	chr8.2:37648124-37648143	MS.gene65609:intron	35.0%
!!	TTGACTAGAGCATTGAAAGT+GGG	-	chr8.2:37649616-37649635	MS.gene65609:intron	35.0%
!!	TTTACTGGTTCAACTGATAC+TGG	-	chr8.2:37648300-37648319	MS.gene65609:CDS	35.0%
!!	TTTGACTAGAGCATTGAAAG+TGG	-	chr8.2:37649615-37649634	MS.gene65609:intron	35.0%
!!!	GAAAAGAAGGGTTGTTTTTG+TGG	-	chr8.2:37647034-37647053	MS.gene65609:intron	35.0%
!!!	TAAGAGTGCTACTCCTTTTT+TGG	-	chr8.2:37646401-37646420	MS.gene65609:intron	35.0%
	AAACTCATGTCTGTGTCAGT+TGG	-	chr8.2:37646654-37646673	MS.gene65609:CDS	40.0%
	AAATTGCAGCACTTGAGACT+TGG	-	chr8.2:37647416-37647435	MS.gene65609:CDS	40.0%
	AAATTTGTAGATGCTGCTGC+TGG	-	chr8.2:37646938-37646957	MS.gene65609:intron	40.0%
	AATTGCAGCACTTGAGACTT+GGG	-	chr8.2:37647417-37647436	MS.gene65609:intron	40.0%
	ATGTACAAATGTTCGAGACC+CGG	+	chr8.2:37648575-37648594	None:intergenic	40.0%
	CAATCTTAAGCCGGTAACTT+TGG	-	chr8.2:37648353-37648372	MS.gene65609:intron	40.0%
	CACAATGACCGTAGAGAATT+AGG	+	chr8.2:37648756-37648775	None:intergenic	40.0%
	CACTTGAGACTTGGGATAAT+GGG	-	chr8.2:37647425-37647444	MS.gene65609:intron	40.0%
	CATTGAAAGTGGGAACAGTT+TGG	-	chr8.2:37649626-37649645	MS.gene65609:intron	40.0%
	CATTTCCAACATTGGATCCA+AGG	-	chr8.2:37647095-37647114	MS.gene65609:CDS	40.0%
	CGGTGTGATGAATGTAATCA+CGG	-	chr8.2:37646519-37646538	MS.gene65609:CDS	40.0%
	GATCACAGACAAACTCCAAA+AGG	+	chr8.2:37647067-37647086	None:intergenic	40.0%
	GCTACATACAAAGCAGACAA+TGG	+	chr8.2:37647797-37647816	None:intergenic	40.0%
	GGATATAAGATGAGTGGACA+GGG	-	chr8.2:37649691-37649710	MS.gene65609:intron	40.0%
	GGTGTTCTTTCTGACATTCA+GGG	-	chr8.2:37648535-37648554	MS.gene65609:CDS	40.0%
	GTTGGGCAGATAAGATTCAT+GGG	-	chr8.2:37647603-37647622	MS.gene65609:intron	40.0%
	TCCAAGCAAACATGAGAAGA+GGG	+	chr8.2:37647715-37647734	None:intergenic	40.0%
	TCTGTAACAATCATCCAGGA+TGG	-	chr8.2:37649165-37649184	MS.gene65609:intron	40.0%
	TCTTCTCATGTTTGCTTGGA+AGG	-	chr8.2:37647715-37647734	MS.gene65609:CDS	40.0%
	TGCAATGTCTGCACATGATA+TGG	+	chr8.2:37647650-37647669	None:intergenic	40.0%
	TGGAAAAGTTGTCCTTCAAC+TGG	-	chr8.2:37648320-37648339	MS.gene65609:CDS	40.0%
	TGGATATAAGATGAGTGGAC+AGG	-	chr8.2:37649690-37649709	MS.gene65609:intron	40.0%
	TGGTCTATTTGGCAGAATCA+CGG	-	chr8.2:37646465-37646484	MS.gene65609:intron	40.0%
	TGGTGTTCTTTCTGACATTC+AGG	-	chr8.2:37648534-37648553	MS.gene65609:CDS	40.0%
	TGTGATGAATGTAATCACGG+TGG	-	chr8.2:37646522-37646541	MS.gene65609:CDS	40.0%
	TTCAAGTCAGGAACAGAACA+AGG	-	chr8.2:37648664-37648683	MS.gene65609:intron	40.0%
	TTCCAAGCAAACATGAGAAG+AGG	+	chr8.2:37647716-37647735	None:intergenic	40.0%
!	AATGTACTTTCAGGTGGGAA+TGG	-	chr8.2:37646809-37646828	MS.gene65609:intron	40.0%
!	ACTTGCACCGATGGTTTAAT+CGG	+	chr8.2:37646890-37646909	None:intergenic	40.0%
!	CACGCAAAGCATTTGATCAA+GGG	-	chr8.2:37647185-37647204	MS.gene65609:intron	40.0%
!	CTTTGCCTTATTCTTCAACC+AGG	-	chr8.2:37648446-37648465	MS.gene65609:intron	40.0%
!	CTTTTGCAGCTTGCATTTAC+TGG	-	chr8.2:37648285-37648304	MS.gene65609:CDS	40.0%
!	TGTTCTGTTCCTGACTTGAA+TGG	+	chr8.2:37648664-37648683	None:intergenic	40.0%
!	TTTGGAGTTTGTCTGTGATC+AGG	-	chr8.2:37647067-37647086	MS.gene65609:CDS	40.0%
!!	CATGGTTGGTACTACTTTTG+TGG	-	chr8.2:37646301-37646320	MS.gene65609:intron	40.0%
!!	GGAGTTTGTTGAGAAAGCAA+AGG	-	chr8.2:37648609-37648628	MS.gene65609:CDS	40.0%
	AACATTGGATCCAAGGACAG+GGG	-	chr8.2:37647102-37647121	MS.gene65609:CDS	45.0%
	AATCTTATCTGCCCAACCTG+GGG	+	chr8.2:37647600-37647619	None:intergenic	45.0%
	AATGGCGCTACACTTGAAAC+TGG	-	chr8.2:37648994-37649013	MS.gene65609:intron	45.0%
	ACATGATATGGTCCATCAGC+TGG	+	chr8.2:37647638-37647657	None:intergenic	45.0%
	AGCCATGCTGGATTCTTCAA+AGG	+	chr8.2:37649775-37649794	None:intergenic	45.0%
	AGTGATTGCTCATGTTGCTG+AGG	-	chr8.2:37647126-37647145	MS.gene65609:CDS	45.0%
	ATGAGAAGAGGGAAGTTCCA+TGG	+	chr8.2:37647704-37647723	None:intergenic	45.0%
	ATTGTCCAAGATTCAGGCAC+TGG	+	chr8.2:37648265-37648284	None:intergenic	45.0%
	CAACATTGGATCCAAGGACA+GGG	-	chr8.2:37647101-37647120	MS.gene65609:CDS	45.0%
	CAGACATTGCACGAACCAAT+TGG	-	chr8.2:37647659-37647678	MS.gene65609:intron	45.0%
	CGCCTAATTCGTTACTATGC+TGG	-	chr8.2:37647493-37647512	MS.gene65609:intron	45.0%
	CTACAGCACGTTTCAAAGCA+CGG	+	chr8.2:37648636-37648655	None:intergenic	45.0%
	CTCATGTTTGCTTGGAAGGT+TGG	-	chr8.2:37647719-37647738	MS.gene65609:CDS	45.0%
	CTCTTTGAATCACCTCACCA+AGG	+	chr8.2:37649531-37649550	None:intergenic	45.0%
	GAAACTGGAGGAGAAAGACT+TGG	-	chr8.2:37649009-37649028	MS.gene65609:intron	45.0%
	GAATCTTATCTGCCCAACCT+GGG	+	chr8.2:37647601-37647620	None:intergenic	45.0%
	GAGATGCTCAGTTCTCAATC+TGG	+	chr8.2:37649404-37649423	None:intergenic	45.0%
	GCACTTGAGACTTGGGATAA+TGG	-	chr8.2:37647424-37647443	MS.gene65609:intron	45.0%
	GGTTGGGCAGATAAGATTCA+TGG	-	chr8.2:37647602-37647621	MS.gene65609:intron	45.0%
	GTAGGAGATCCATTCAAGTC+AGG	-	chr8.2:37648652-37648671	MS.gene65609:intron	45.0%
	GTGATTGCTCATGTTGCTGA+GGG	-	chr8.2:37647127-37647146	MS.gene65609:CDS	45.0%
	TAAGCTGGAAACTTAGGCCA+CGG	+	chr8.2:37647211-37647230	None:intergenic	45.0%
	TACAGCACGTTTCAAAGCAC+GGG	+	chr8.2:37648635-37648654	None:intergenic	45.0%
	TCAGGTGGGAATGGTTATCT+GGG	-	chr8.2:37646818-37646837	MS.gene65609:intron	45.0%
	TCCCTCTTCTCATGTTTGCT+TGG	-	chr8.2:37647711-37647730	MS.gene65609:CDS	45.0%
	TGAATCTTATCTGCCCAACC+TGG	+	chr8.2:37647602-37647621	None:intergenic	45.0%
	TGTACATGAACGTGTGCATG+AGG	-	chr8.2:37648588-37648607	MS.gene65609:CDS	45.0%
	TTCAGGTGGGAATGGTTATC+TGG	-	chr8.2:37646817-37646836	MS.gene65609:intron	45.0%
	TTGCTGGTCAGATCATTCCA+TGG	-	chr8.2:37647684-37647703	MS.gene65609:intron	45.0%
	TTTACCGCGAGAAAACAAGG+AGG	+	chr8.2:37645998-37646017	None:intergenic	45.0%
!	AGGAGAAAGACTTGGTAGCA+AGG	-	chr8.2:37649017-37649036	MS.gene65609:intron	45.0%
!	CACCTTTGAAGAATCCAGCA+TGG	-	chr8.2:37649770-37649789	MS.gene65609:CDS	45.0%
!	GATGCTGCAATTCCTTTTGG+TGG	-	chr8.2:37649670-37649689	MS.gene65609:intron	45.0%
!	GCACGCAAAGCATTTGATCA+AGG	-	chr8.2:37647184-37647203	MS.gene65609:intron	45.0%
!	GGAGAAAGACTTGGTAGCAA+GGG	-	chr8.2:37649018-37649037	MS.gene65609:intron	45.0%
!	TCAAAGTATGGTCTTGCAGC+AGG	-	chr8.2:37649559-37649578	MS.gene65609:intron	45.0%
!	TTGATTGGTTCAAGGGACCT+TGG	-	chr8.2:37649511-37649530	MS.gene65609:intron	45.0%
!!	TGCTTTTAGCTGCCAGTTGA+AGG	+	chr8.2:37648335-37648354	None:intergenic	45.0%
	AAGCATTTGATCAAGGGCCG+TGG	-	chr8.2:37647191-37647210	MS.gene65609:intron	50.0%
	AGGAACAGAACAAGGTCCTC+AGG	-	chr8.2:37648672-37648691	MS.gene65609:intron	50.0%
	AGTGGACAGGGCAGAGAAAA+AGG	-	chr8.2:37649703-37649722	MS.gene65609:intron	50.0%
	ATCTGACCAGCAACGCCAAT+TGG	+	chr8.2:37647677-37647696	None:intergenic	50.0%
	CACACTCAGCTCCTAATCGA+TGG	-	chr8.2:37646914-37646933	MS.gene65609:intron	50.0%
	CCAACATTGGATCCAAGGAC+AGG	-	chr8.2:37647100-37647119	MS.gene65609:CDS	50.0%
	CCACCAAGCTCCAAAGTTAC+CGG	+	chr8.2:37648366-37648385	None:intergenic	50.0%
	CCTGTCCTTGGATCCAATGT+TGG	+	chr8.2:37647103-37647122	None:intergenic	50.0%
	CGAATTAGGCGAACCAGCAT+TGG	+	chr8.2:37647484-37647503	None:intergenic	50.0%
	CGTTACTATGCTGGTACGTG+CGG	-	chr8.2:37647502-37647521	MS.gene65609:intron	50.0%
	TGCTGCTCTTGCTAGTCACA+TGG	-	chr8.2:37648011-37648030	MS.gene65609:CDS	50.0%
	TTGCAATCTGCATGCCATCC+TGG	+	chr8.2:37649182-37649201	None:intergenic	50.0%
	TTGGAAGGTTGGACCTGCAT+TGG	-	chr8.2:37647730-37647749	MS.gene65609:CDS	50.0%
!	CATCGATTAGGAGCTGAGTG+TGG	+	chr8.2:37646916-37646935	None:intergenic	50.0%
!!	AAGCCGGTAACTTTGGAGCT+TGG	-	chr8.2:37648360-37648379	MS.gene65609:intron	50.0%
!!	CGGTAACTTTGGAGCTTGGT+GGG	-	chr8.2:37648364-37648383	MS.gene65609:intron	50.0%
!!	CGTGCTTTGAAACGTGCTGT+AGG	-	chr8.2:37648634-37648653	MS.gene65609:intron	50.0%
!!!	TCCACCTCCTTGTTTTCTCG+CGG	-	chr8.2:37645991-37646010	MS.gene65609:CDS	50.0%
	ACCGCGAGAAAACAAGGAGG+TGG	+	chr8.2:37645995-37646014	None:intergenic	55.0%
	AGGGACAATGTTGCTGTGCC+GGG	-	chr8.2:37648554-37648573	MS.gene65609:CDS	55.0%
	CACGAACCAATTGGCGTTGC+TGG	-	chr8.2:37647668-37647687	MS.gene65609:intron	55.0%
	CAGGGACAATGTTGCTGTGC+CGG	-	chr8.2:37648553-37648572	MS.gene65609:CDS	55.0%
	CCTGTCCAGTGCCTGAATCT+TGG	-	chr8.2:37648257-37648276	MS.gene65609:CDS	55.0%
	GCAATCACTTCCCCTGTCCT+TGG	+	chr8.2:37647115-37647134	None:intergenic	55.0%
	GGCGCTACACTTGAAACTGG+AGG	-	chr8.2:37648997-37649016	MS.gene65609:intron	55.0%
	GGTGGGAATGGTTATCTGGG+TGG	-	chr8.2:37646821-37646840	MS.gene65609:intron	55.0%
	TGCTTTGCGTGCAGCTGCAA+CGG	+	chr8.2:37647176-37647195	None:intergenic	55.0%
	TGGTTCAAGGGACCTTGGTG+AGG	-	chr8.2:37649516-37649535	MS.gene65609:intron	55.0%
	TTGTAGGCTGGACTTCCTCC+TGG	-	chr8.2:37647949-37647968	MS.gene65609:CDS	55.0%
!!	CCAAGATTCAGGCACTGGAC+AGG	+	chr8.2:37648260-37648279	None:intergenic	55.0%
!!	CCGGTAACTTTGGAGCTTGG+TGG	-	chr8.2:37648363-37648382	MS.gene65609:intron	55.0%
!!	TCTGGCTTTGGTCCAACTGC+AGG	-	chr8.2:37647988-37648007	MS.gene65609:CDS	55.0%
	CTATGCTGGTACGTGCGGTG+TGG	-	chr8.2:37647507-37647526	MS.gene65609:intron	60.0%
	GCAAGAGCAGCACCTGCAGT+TGG	+	chr8.2:37648003-37648022	None:intergenic	60.0%
	GGGCTCACAGTTCCAGCTGA+TGG	-	chr8.2:37647623-37647642	MS.gene65609:intron	60.0%
	GTGTTGCCACACGCCAATGC+AGG	+	chr8.2:37647746-37647765	None:intergenic	60.0%
!	GTTGGACCTGCATTGGCGTG+TGG	-	chr8.2:37647737-37647756	MS.gene65609:CDS	60.0%
!!	GTGTGGTCGACTTGCACCGA+TGG	+	chr8.2:37646899-37646918	None:intergenic	60.0%
	GCTGGTACGTGCGGTGTGGA+AGG	-	chr8.2:37647511-37647530	MS.gene65609:intron	65.0%
	GGTACGTGCGGTGTGGAAGG+TGG	-	chr8.2:37647514-37647533	MS.gene65609:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	37645931	37649798	37645931	ID=MS.gene65609
chr8.2	mRNA	37645931	37649798	37645931	ID=MS.gene65609.t1;Parent=MS.gene65609
chr8.2	exon	37649717	37649798	37649717	ID=MS.gene65609.t1.exon1;Parent=MS.gene65609.t1
chr8.2	CDS	37649717	37649798	37649717	ID=cds.MS.gene65609.t1;Parent=MS.gene65609.t1
chr8.2	exon	37648770	37648907	37648770	ID=MS.gene65609.t1.exon2;Parent=MS.gene65609.t1
chr8.2	CDS	37648770	37648907	37648770	ID=cds.MS.gene65609.t1;Parent=MS.gene65609.t1
chr8.2	exon	37648498	37648640	37648498	ID=MS.gene65609.t1.exon3;Parent=MS.gene65609.t1
chr8.2	CDS	37648498	37648640	37648498	ID=cds.MS.gene65609.t1;Parent=MS.gene65609.t1
chr8.2	exon	37648215	37648368	37648215	ID=MS.gene65609.t1.exon4;Parent=MS.gene65609.t1
chr8.2	CDS	37648215	37648368	37648215	ID=cds.MS.gene65609.t1;Parent=MS.gene65609.t1
chr8.2	exon	37647897	37648126	37647897	ID=MS.gene65609.t1.exon5;Parent=MS.gene65609.t1
chr8.2	CDS	37647897	37648126	37647897	ID=cds.MS.gene65609.t1;Parent=MS.gene65609.t1
chr8.2	exon	37647685	37647774	37647685	ID=MS.gene65609.t1.exon6;Parent=MS.gene65609.t1
chr8.2	CDS	37647685	37647774	37647685	ID=cds.MS.gene65609.t1;Parent=MS.gene65609.t1
chr8.2	exon	37647262	37647435	37647262	ID=MS.gene65609.t1.exon7;Parent=MS.gene65609.t1
chr8.2	CDS	37647262	37647435	37647262	ID=cds.MS.gene65609.t1;Parent=MS.gene65609.t1
chr8.2	exon	37647036	37647173	37647036	ID=MS.gene65609.t1.exon8;Parent=MS.gene65609.t1
chr8.2	CDS	37647036	37647173	37647036	ID=cds.MS.gene65609.t1;Parent=MS.gene65609.t1
chr8.2	exon	37646652	37646789	37646652	ID=MS.gene65609.t1.exon9;Parent=MS.gene65609.t1
chr8.2	CDS	37646652	37646789	37646652	ID=cds.MS.gene65609.t1;Parent=MS.gene65609.t1
chr8.2	exon	37646485	37646546	37646485	ID=MS.gene65609.t1.exon10;Parent=MS.gene65609.t1
chr8.2	CDS	37646485	37646546	37646485	ID=cds.MS.gene65609.t1;Parent=MS.gene65609.t1
chr8.2	exon	37645931	37646204	37645931	ID=MS.gene65609.t1.exon11;Parent=MS.gene65609.t1
chr8.2	CDS	37645931	37646204	37645931	ID=cds.MS.gene65609.t1;Parent=MS.gene65609.t1

Gene Sequence

>MS.gene65609

ATGGCTTCTTCAATCAAAATCTCAAGGCTTATTTCTCGATCATTTTCTTCTGCTTCTTCTTCCACCTCCTTGTTTTCTCGCGGTAAATTATATCTTTACTTAATCACATATTTTATGATTATTTTCATATATCATCTAACATAATTATGTGACTTTTGTTTGTGTTTGTGTTTGTGTTTTTTAGTTTTTTCTTTTAAATTTGTCTTTTCTAAGAAAATGATTTTCTGGGGTTGTTTTGAATTTGATTGATGATAGGATCACAATCAATGTTTTGAATTTTGAGAAAAATAAGATAAGATAAGATTTTGGAAATTTTGTCATTGTAATCTGTGTGACATGAAAGAAAGGTTTCTTTTTCTTTCATTAATTGCATGGTTGGTACTACTTTTGTGGTCTCGTTTTCATAACTGTACTTTTTGATTCTCTCACTTCACTTATAGTTATCTGCTGCTACTTTTTTTTTTTTTTAATAAGAGTGCTACTCCTTTTTTGGCTATAGTAATATGAAAATTGGTTTGTACATGTTTAGATTGATGGTCTATTTGGCAGAATCACGGTGTGCCACCTTGAAATGCATGTTTAAATTAACGGTGTGATGAATGTAATCACGGTGGCATCGTGATTTTGACAAATTGACACTAGATCCAAAGATGGACTTAACAAAAATAAGAGTGACACCGTGATTTCGTCAATCTCATTGTGATACCGAATATTTCTTTCTAAAAACTCATGTCTGTGTCAGTTGGAGATGATGTTATCTTGATTTCAATTGGTGATTAAAAGTGGTGTTTCAGTTTATAATTTTCATTAGGGGTTTAGGTTTTTTAAAGGCATTGATTGATGCTCAATGATAAATTGAATATTGAAAGGAAAAAAAAAATGTACTTTCAGGTGGGAATGGTTATCTGGGTGGATTACTCTCTAAATACAGCACTGCTGCTGCCATTGATGAAAAACCGATTAAACCATCGGTGCAAGTCGACCACACTCAGCTCCTAATCGATGGCAAATTTGTAGATGCTGCTGCTGGTGAGTGTTTTTTTTATTTTATTTTATTCTTTTTATACAATTTTGATTAGGAAAGAAAAGAGGATTAACTAAAGGAAAAGAAGGGTTGTTTTTGTGGTATCTTACCTTTTGGAGTTTGTCTGTGATCAGGTAAAACATTTCCAACATTGGATCCAAGGACAGGGGAAGTGATTGCTCATGTTGCTGAGGGTCACTCTGAAGATGTTGATAGAGCCGTTGCAGCTGCACGCAAAGCATTTGATCAAGGGCCGTGGCCTAAGTTTCCAGCTTATGTATGATCATTGCTTCTTCGTTTTTCTGTTTGTGATTTTTCTATTGTTTGTCAAGTCATTATATTTGGTTCAACTTCAATGAATTTGCGGTTGAGTCCACTTTTGTTTTTCTGTTTCTGTGTTTTATAGGAAAGGCAAAAGATAATGTTGCGTGCTGCTGATTTGATTGAAAAGCATAACGATGAAATTGCAGCACTTGAGACTTGGGATAATGGGAAACCATATGAACAATCTGCTCAGATTGAAATTCCAATGCTGGTTCGCCTAATTCGTTACTATGCTGGTACGTGCGGTGTGGAAGGTGGTACTAACAGTTCTTCTCTTTGTTTTGTAAAACTACACTCACACTTGTATTTTCATATTTACCCCAGGTTGGGCAGATAAGATTCATGGGCTCACAGTTCCAGCTGATGGACCATATCATGTGCAGACATTGCACGAACCAATTGGCGTTGCTGGTCAGATCATTCCATGGAACTTCCCTCTTCTCATGTTTGCTTGGAAGGTTGGACCTGCATTGGCGTGTGGCAACACAGTCGTTCTAAAAACAGCTGAACAGACACCATTGTCTGCTTTGTATGTAGCAAAACTATTTCACGAGGTTTGATACATTCACCAATCTTGTTTTAATTTTTATAAATCAATATAAAAGCATTGTTTTTGGTGGAGTTTATTTTAACCATAAAATATATCATCTTTGCTAATTTGTAATCTTTCTTGTAGGCTGGACTTCCTCCTGGTGTTTTGAACATAATATCTGGCTTTGGTCCAACTGCAGGTGCTGCTCTTGCTAGTCACATGGATGTTGATAAGGTACTAAAATATTAAGTTCTGCACTTGTGTTTTAATGTAATTATAAGTTGCTAGTATATTCAAACTGACTTCACTCCATGTACCGACATTTTAGATTGAAGGCATGTCTAGTGTCTGACATGTGTTAGTGTCTGACACTTACACGACACTGACATATATGATTACATTTAATCACTTCTATTTCTTATATTATTACTAGTCTCTATGTATCGCCTGTCCAGTGCCTGAATCTTGGACAATCTTTTGCAGCTTGCATTTACTGGTTCAACTGATACTGGAAAAGTTGTCCTTCAACTGGCAGCTAAAAGCAATCTTAAGCCGGTAACTTTGGAGCTTGGTGGGAAATCTCCGTTTATTGTTTGCGAAGATGCTGATATAGATGAAGCTGTTGAGCTAGCACACTTTGCCTTATTCTTCAACCAGGTATGTTCAATCATAAAGTTCTTGTTATTAAACAATTGTCTCTCGTATAAATCAAATAGCACTGATTGGTGTTCTTTCTGACATTCAGGGACAATGTTGCTGTGCCGGGTCTCGAACATTTGTACATGAACGTGTGCATGAGGAGTTTGTTGAGAAAGCAAAGGCCCGTGCTTTGAAACGTGCTGTAGGAGATCCATTCAAGTCAGGAACAGAACAAGGTCCTCAGGTAAATTACAACGAATAATTTGAGCGACATCTTCATGATTAATCTTAGAGCTTGGTTTTCCTAATTCTCTACGGTCATTGTGATGTTCAGATACTAGAGGCTTCACAAAATTGAAATGCATAGAAGAAATCAAGAATATACTACATTGCTACTACTTTGATATTCTCTTGTTAAACCTTAATGTTTGTGACTCTTTTGAAATGTCTGAATATATACTGAAATATTTCTTATGATCCAAACAATCAGATTGATTCAAAGCAATTTGAGAAAATATTGAAGTATATTAGATCTGGCGTTGATAATGGCGCTACACTTGAAACTGGAGGAGAAAGACTTGGTAGCAAGGGCTACTATATTCAGCCTACTGTCTTCTCAAATGTTCAGGTGTGTGTGTGCGTGCGCGCGTTCACGCTTATATTCTCATCTTTGCAAAGGATTTTGAAAATACAATTCCTAATGTTTTTAATTGTATCTGTAACAATCATCCAGGATGGCATGCAGATTGCAAAAGAAGAGATATTTGGTCCAGTACAAACCATATTGAAATTCAAGTGAGACCAATGAGTCTTGATTTGTAGAGAATGATATTTTCTAGTTTCAAATATATTTTTAGTCCCTTTAATGCACCCTTCATCAAATGTAGTCTATATAAAAATTTCAGTGTTGTTTAGCTCCCAATTTGACGATTTTTGCAAAAGTTGAAATCACCAGATTGAGAACTGAGCATCTCTAGAAAATTTTATAAGAGGTGTATTTTACAAGGACTAAAGACATATATTGAACCCTATTTTTATGTGCAATGCAATTAATTTTAATGTTGATTGGTTCAAGGGACCTTGGTGAGGTGATTCAAAGAGCAAACAATTCAAAGTATGGTCTTGCAGCAGGAGTTTTCACAAAGAACATAGACACTGCAAATACTTTGACTAGAGCATTGAAAGTGGGAACAGTTTGGGTAAATTGCTTTGATACATTTGATGCTGCAATTCCTTTTGGTGGATATAAGATGAGTGGACAGGGCAGAGAAAAAGGAGAATATAGTCTTAAAAACTACTTGCAAGTGAAGGCTGTTGTTACACCTTTGAAGAATCCAGCATGGCTTTGA

Protein sequence

>MS.gene65609.t1

MASSIKISRLISRSFSSASSSTSLFSRGGNGYLGGLLSKYSTAAAIDEKPIKPSVQVDHTQLLIDGKFVDAAAGKTFPTLDPRTGEVIAHVAEGHSEDVDRAVAAARKAFDQGPWPKFPAYERQKIMLRAADLIEKHNDEIAALETWDNGKPYEQSAQIEIPMLVRLIRYYAGWADKIHGLTVPADGPYHVQTLHEPIGVAGQIIPWNFPLLMFAWKVGPALACGNTVVLKTAEQTPLSALYVAKLFHEAGLPPGVLNIISGFGPTAGAALASHMDVDKLAFTGSTDTGKVVLQLAAKSNLKPVTLELGGKSPFIVCEDADIDEAVELAHFALFFNQGQCCCAGSRTFVHERVHEEFVEKAKARALKRAVGDPFKSGTEQGPQIDSKQFEKILKYIRSGVDNGATLETGGERLGSKGYYIQPTVFSNVQDGMQIAKEEIFGPVQTILKFKDLGEVIQRANNSKYGLAAGVFTKNIDTANTLTRALKVGTVWVNCFDTFDAAIPFGGYKMSGQGREKGEYSLKNYLQVKAVVTPLKNPAWL