AlfalfaGEDB | MS.gene64463

M. sativa cultivar XinJiangDaYe / MS.gene64463

KEGG: K14085
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016620 GO:0016903 GO:0055114
PFAM: PF00171.25

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64463.t1	XP_003595308.1	99.2	491	4	0	1	491	1	491	2.20E-278	967.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64463.t1	P25795	89.2	491	52	1	1	491	1	490	3.1e-252	872.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64463.t1	G7IGF7	99.2	491	4	0	1	491	1	491	1.6e-278	967.6

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene009119	MS.gene64463	PPI
MS.gene013819	MS.gene64463	PPI
MS.gene015742	MS.gene64463	PPI
MS.gene015744	MS.gene64463	PPI
MS.gene016051	MS.gene64463	PPI
MS.gene026652	MS.gene64463	PPI
MS.gene033206	MS.gene64463	PPI
MS.gene035652	MS.gene64463	PPI
MS.gene038961	MS.gene64463	PPI
MS.gene042286	MS.gene64463	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64463.t1	MTR_2g042330	99.185	491	4	0	1	491	1	491	0.0	991
MS.gene64463.t1	MTR_2g067660	30.000	490	322	8	3	487	36	509	2.64e-66	223
MS.gene64463.t1	MTR_8g064170	30.444	473	310	11	23	488	67	527	1.44e-58	202
MS.gene64463.t1	MTR_8g083530	29.958	474	314	12	24	488	25	489	5.09e-57	197
MS.gene64463.t1	MTR_3g078170	29.684	475	312	11	25	488	65	528	6.89e-55	193
MS.gene64463.t1	MTR_3g078550	29.375	480	312	11	25	487	11	480	2.29e-54	191
MS.gene64463.t1	MTR_4g107040	29.053	475	315	12	25	488	67	530	8.30e-53	187
MS.gene64463.t1	MTR_3g078490	28.750	480	315	11	25	487	41	510	7.51e-52	184
MS.gene64463.t1	MTR_4g106510	30.185	487	299	13	25	487	11	480	1.40e-51	183
MS.gene64463.t1	MTR_3g078550	29.742	427	280	9	71	487	34	450	6.18e-49	175

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64463.t1	AT1G54100	78.819	491	103	1	1	491	1	490	0.0	810
MS.gene64463.t1	AT1G54100	78.819	491	103	1	1	491	1	490	0.0	810
MS.gene64463.t1	AT1G79440	30.020	493	328	7	1	487	34	515	5.08e-60	206
MS.gene64463.t1	AT1G23800	30.258	466	303	12	33	488	67	520	2.40e-56	197
MS.gene64463.t1	AT3G24503	27.579	475	329	9	25	491	23	490	2.16e-55	193
MS.gene64463.t1	AT1G74920	28.216	482	315	11	25	487	11	480	3.34e-55	193
MS.gene64463.t1	AT1G74920	28.092	477	317	10	25	487	11	475	2.83e-54	190
MS.gene64463.t1	AT3G48000	28.421	475	318	12	25	488	61	524	2.26e-52	186
MS.gene64463.t1	AT3G48170	28.333	480	317	11	25	487	11	480	9.56e-51	181
MS.gene64463.t1	AT2G14170	25.105	474	333	8	23	485	11	473	4.37e-37	143

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Find 132 sgRNAs with CRISPR-Local

Find 349 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGTGAATGAAAGATT+TGG	0.393915	2.4:-47957238	MS.gene64463:CDS
AAAATTCTCGCAGAAGGAAT+TGG	0.378286	2.4:-47960367	MS.gene64463:CDS
AAACTTCAAGAAGGGCATTG+CGG	0.671686	2.4:-47956854	MS.gene64463:CDS
AAAGAGGTGAATATTGCCCC+AGG	0.617216	2.4:+47957721	None:intergenic
AAATGCTTACTGGAGTTAAG+TGG	0.600833	2.4:-47957214	MS.gene64463:CDS
AAATTCTCGCAGAAGGAATT+GGG	0.364193	2.4:-47960366	MS.gene64463:CDS
AACAAATGGAGCTGAGATTG+GGG	0.570910	2.4:-47956132	MS.gene64463:CDS
AACAAGAGTCCCTTTCTCCA+GGG	0.580093	2.4:+47956906	None:intergenic
AACATCGGTTGTTTCATCAA+TGG	0.487652	2.4:-47961305	MS.gene64463:CDS
AACTGTGATAGATTATGAAG+AGG	0.621122	2.4:-47960644	MS.gene64463:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAAAAAGCCTTCCAGTA+TGG	-	chr2.4:47957301-47957320	MS.gene64463:intron	30.0%
AAAAAAAAGCCTTCCAGTAT+GGG	-	chr2.4:47957302-47957321	MS.gene64463:intron	30.0%
AAAAGTCATGAAGTTCATCA+TGG	+	chr2.4:47959416-47959435	None:intergenic	30.0%
AACTGTGATAGATTATGAAG+AGG	-	chr2.4:47956722-47956741	MS.gene64463:intron	30.0%
AATGTGAACATACCAACAAA+TGG	-	chr2.4:47961220-47961239	MS.gene64463:intron	30.0%
ACAAAATTGGACATAACACA+TGG	+	chr2.4:47960602-47960621	None:intergenic	30.0%
ACACAAAATCTGACAAATAG+AGG	+	chr2.4:47959962-47959981	None:intergenic	30.0%
ACTGTACATCCAATTCAATT+TGG	+	chr2.4:47959813-47959832	None:intergenic	30.0%
ACTGTGATAGATTATGAAGA+GGG	-	chr2.4:47956723-47956742	MS.gene64463:intron	30.0%
ATAAACATTGGTGTGTGTAT+AGG	-	chr2.4:47957874-47957893	MS.gene64463:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	47956001	47961387	47956001	ID=MS.gene64463
chr2.4	mRNA	47956001	47961387	47956001	ID=MS.gene64463.t1;Parent=MS.gene64463
chr2.4	exon	47961250	47961387	47961250	ID=MS.gene64463.t1.exon1;Parent=MS.gene64463.t1
chr2.4	CDS	47961250	47961387	47961250	ID=cds.MS.gene64463.t1;Parent=MS.gene64463.t1
chr2.4	exon	47960603	47960689	47960603	ID=MS.gene64463.t1.exon2;Parent=MS.gene64463.t1
chr2.4	CDS	47960603	47960689	47960603	ID=cds.MS.gene64463.t1;Parent=MS.gene64463.t1
chr2.4	exon	47960357	47960488	47960357	ID=MS.gene64463.t1.exon3;Parent=MS.gene64463.t1
chr2.4	CDS	47960357	47960488	47960357	ID=cds.MS.gene64463.t1;Parent=MS.gene64463.t1
chr2.4	exon	47959264	47959336	47959264	ID=MS.gene64463.t1.exon4;Parent=MS.gene64463.t1
chr2.4	CDS	47959264	47959336	47959264	ID=cds.MS.gene64463.t1;Parent=MS.gene64463.t1
chr2.4	exon	47959070	47959092	47959070	ID=MS.gene64463.t1.exon5;Parent=MS.gene64463.t1
chr2.4	CDS	47959070	47959092	47959070	ID=cds.MS.gene64463.t1;Parent=MS.gene64463.t1
chr2.4	exon	47958912	47958975	47958912	ID=MS.gene64463.t1.exon6;Parent=MS.gene64463.t1
chr2.4	CDS	47958912	47958975	47958912	ID=cds.MS.gene64463.t1;Parent=MS.gene64463.t1
chr2.4	exon	47958666	47958711	47958666	ID=MS.gene64463.t1.exon7;Parent=MS.gene64463.t1
chr2.4	CDS	47958666	47958711	47958666	ID=cds.MS.gene64463.t1;Parent=MS.gene64463.t1
chr2.4	exon	47957642	47957822	47957642	ID=MS.gene64463.t1.exon8;Parent=MS.gene64463.t1
chr2.4	CDS	47957642	47957822	47957642	ID=cds.MS.gene64463.t1;Parent=MS.gene64463.t1
chr2.4	exon	47957102	47957278	47957102	ID=MS.gene64463.t1.exon9;Parent=MS.gene64463.t1
chr2.4	CDS	47957102	47957278	47957102	ID=cds.MS.gene64463.t1;Parent=MS.gene64463.t1
chr2.4	exon	47956840	47957004	47956840	ID=MS.gene64463.t1.exon10;Parent=MS.gene64463.t1
chr2.4	CDS	47956840	47957004	47956840	ID=cds.MS.gene64463.t1;Parent=MS.gene64463.t1
chr2.4	exon	47956542	47956682	47956542	ID=MS.gene64463.t1.exon11;Parent=MS.gene64463.t1
chr2.4	CDS	47956542	47956682	47956542	ID=cds.MS.gene64463.t1;Parent=MS.gene64463.t1
chr2.4	exon	47956360	47956457	47956360	ID=MS.gene64463.t1.exon12;Parent=MS.gene64463.t1
chr2.4	CDS	47956360	47956457	47956360	ID=cds.MS.gene64463.t1;Parent=MS.gene64463.t1
chr2.4	exon	47956001	47956196	47956001	ID=MS.gene64463.t1.exon13;Parent=MS.gene64463.t1
chr2.4	CDS	47956001	47956196	47956001	ID=cds.MS.gene64463.t1;Parent=MS.gene64463.t1

Gene Sequence

>MS.gene64463

ATGGGTTCTGATCAGAGTAACAACTTGGAATTTTTGAAAGAGATCGGTTTAGGTTCAACTAACATCGGTTGTTTCATCAATGGTCAGTGGAAAGCCAATGGTTCTTCTGTTACTTCTGTTAATCCTTCCACCAATCAGGTCACAATTTTTCTCACTTTTTTTTTTTTACTTTTTTTTTTAATTTTTGAAAAGTTTTTTTTTTTTTTGCAACTTTGCATGCATGAAAGAACTGTTGAATTATGAAACTTTGATGTTAAAACTGAAATGAAACTGTTACAAGTAAAGATCTTTGTTAAATTTAGATAGCTTAGTGAGTATAGCTTTGTAAAATGATCATTAATGTTATCTTAATTAATAAAAAGTTTACTCTTGCCTAATTTTGTTTTTAAGAAGGGAAGATATTTTTTTTTATTTTTTTTGTTTTTGTTTTTTACTAAACCTGAGTGATGAATGAAAAAAGTTTAGTGAATTTGAATTTGAGCTTGATTGATGATTGAATTTTAAGTGCATATTTGGATTTGCGATGAATTTAGCCGAATCACGGTGTTTTTATAAATTTAGTGAAGCTTAGAATTGTAGCTTTTGCCAAAATTAGTTGGATTTCAAATCAATACAAACAAGCAGTTAGTTGTTTTCATGGTTTTTGGCGATTTTGGATTTCATTTTTATGTATCTGATTGTTGTTGCTTTGAGTTTAGACTATTGCTACGGTGACCGAAGCAACTGTGATAGATTATGAAGAGGGATTGCAAGCATGTAGTGAAGCAGCCAAGACATGGATGACTGTGAGTTTTTCTCTCTTTTTTGATATAATTGTGAATTTGCGATAGCCGATGGCTTATACTGAATTTAGGTACAGTTGGAAACTAAATGAGGATGAACTTGTGACGTTCTTGCAGATTCCGGCACCCAAAAGAGGTGAGATTGTAAGGCAGATTGGGGATGCATTGAGAGCTAAATTGGATCCGTTGGGTAGGTTGGTGGCTCTTGAGATGGGAAAAATTCTCGCAGAAGGAATTGGGGAAGTTCAGGTATGCCAAATCTGTTTGTTAAGCAATTTTTCGTAACATGTTGTGTTTGACAGTACGTAAGTCAAATCGGTGGTAACCAAGTAATGCATTATCAGATATCATGAACTAAGTACCTTGTACTATTTTGGTGTAATGTCATCCAAATCTTGGCCCAAAACGTGGGTTTGTTTTTGCTGTTAGGGCCGACAAATGCGTCTCTAAAGCTTCGTCCAATGAAGCATTTTCTGCATAGTTGCATTCTTTCCATCAATTTTCATTCACGACAAAGAAAAAAAAAAGCCTTCCAGTATGGGCATCTAAAATGTAACTGGTTGCATAAAAATCCTGAAATCCATTGCAGTCAACAAGGTTAACTGGAACCGTACTTTTGAAAATTGTTAACTTGGTAAAAAATAAGTGGACATACTGGAGAGTGAAAAGGCTTCCCGTTATTCAGAATGTTTAAGAATTGTAATGAACTTTTTTCATGTTATACTCTACATTTTCGAATATAACCAAGTCGACTTAACTATTTCAGGAGGAAAATATGAAATTGATGTGATTGGGTAGAGTGACCATCACGAACGGTTGATTATGTAATATGAAAAGTAATGTTGGGAGCTCTTAAATTTTCCCAGCACTTGCTAATTTAGTAACCAATTTTGCCAGTGGGGGAGATTGCCATCTTTTAAATACTAGTTGTACTATCACCAACCCTATATCTTTTTGGTGATAAATTTGACTATCTATTCATTTTAGAATTGAGCCGCGTTAACAGCATTTTCATGGCCTATGTCCTGTATTTGGTTCTCCGAGGATAGTCACTTTTCAAAGTTCCTTCATTTTCTTTCTTGAGGCATTGCATAAACATTGGTGTGTGTATAGGCTTACTGCTATATGATGCAATCTAGTACTTTTTCTCGATACACAGAATTAACAATAATCATATACTCTATAAAAGAGAAAATTATATTATATATAATATTTGTTCTCACATTTGTATGCGATATAGTTTGTAACCTTTCATGCTATTTGTTACAGGAAATTATTGATATGTGTGATTATGCTGTTGGGCTAAGCCGACAATTGAATGGATCAATTATCCCATCAGAACGTAAGATTTTTTATCTTTATCAATTGTTCATCGTTTCTTTATAATAATACTCAGGGTTATAAAATTGTTTTAAAAAAATTTGTTCACAGACACTTTTGTTTCGATGCTAATTTCTTTTCATGATGTTCTGGTTCTGAGTTAATTTTTTCGTTAAACTTTCTTTATTTACAGGTCCGGATCATATGATGTTTGAGGTTCGATATCAAACCGTGATTATTTTGTAACTTCTTCACCATTATATCATAGGGGGTTTAGTTATGAACTGTATTGTGTGCTCTTACACTACAGGTATGGAACCCACTAGGAATAGTTGGTGTAATCACTGCTTTCAACTTTCCATGTGCTGTTCTAGGTATACTTTTCTTCATCTATCTCAACACACGCAGTGATAAATTTCTGGTCATTCTATTAAGAATGTGTTGCTTATCAAAAGAAAATTTCTTAGGAATGTATTCCAAACATCAAGAAAACTATCTGCACTTACTTTTTATACTTCGAGTTATTCTGGTGATTAAAATGTTGTATAACCTGTTCATTTCTGTTGTGTCTCAGGATGGAATGCTTGCATTGCATTAGTCTGTGGTAACTGTGTAGTCTGGTAAGTTCACATATTACTAGGCATGTGTGTGTGTGTGTAGATTTCAGACCAATTTTCTTGATCTTCTTTTGTCTACTATTTTCTCTTGACTTGAATGGTATTTATACTGTAAACTTTATGCGATCAATACTTCAATTCTTGATGCATACTGTTCAATGTATGTGATTTATCACCATCTTTCATTTGGTATCTCGTAAAAGTTGACTTTCCTACAATTGCACACACTGTTAAGGCAACTTCTCTGTGTTGGCTGAATATATCTTAAATGACACAGTTGGTGGACTCAAGCTGTAAGTTTTGGTGACAACTTGACCACCGATTTCTCCAATTGCACTTCTTTTGAATTTGTTTCACTTAGTAGTCAGTACAAAATTTCCAATAACATCAATCCGTAAAATAGCCTACCCCACCAAGTGAGATAAGGTTGGTGGTGCGCTTGGTTGTTATTGTTCATGAAATATACTTTAGTGGTTAAAGTGCTATGTATTTTTGTTCTCTTATGTGGCATCCTCATAATCTGATCTCTTTACGCAATGGTTGCGTAATTTATCCTTGCCTCCGAGGACTTCTTTCTCCCTCTTCCCATTACAATATGTGTATCTGTATTCTGTATAATATGCATAAATGGCTTAGAAAGGAGGGTTATCAAAAGGGATTAAAATGAAAAAAAATTATCTTGATTGTTGCTGACCATGATGAACTTCATGACTTTTATGCAAATCTTTGTTGAAATTTTATGTGATATCTTGTCAGCTTCTTCTTCTTCTAGTGTGTTTCAAATTTTCAATAAAAATTTTGTCGTCTTTTCATTACAATTTTTTCTTACTACCACACGAATTACAGGAAGGGAGCTCCAACTACACCATTGATAACTATTGCTATTACAAAGCTAATATCTGAAGTTATTGAGAGGAACAAATTACCTGGGGCAATATTCACCTCTTTCTGTGGAGGTGCTGATATTGGGCAGGCAATATCGAAAGACAGACGCATTCCACTGGTTTCATTTACCGGAAGCTCAAAGGTAGTTTCTTTGAGTACTTGATCTCTAAACATAATGTTTTTAACAGGTGACATAATTAATTAACCAAATTGAATTGGATGTACAGTTTTCCTCTTTTTATTAAAAATGTAGTTATCACCCAAGTTTCTCTGTCATTAGGTCTTATTACAATAACTATTTATAAAGATATGTAATATTTAACACCCTATAGCATCATCTTGGTAGTACATATACCTCTATTTGTCAGATTTTGTGTAATCATGTATATCTGGCCTCCTATTCAAACATGCTACGTTGAGATACAGTGCCGAATGTTCACTTCAGTATGATTTGTAGATTAAAATCTATACGTATTGAGCTGATAATGAAATGCTCCTATTGTAGGTGGGTGCGGAGGTACAGAAAACAGTGAATGAAAGATTTGGCAAATGCTTACTGGAGTTAAGTGGTAACAATGCAATTATAGTCATGGATGATGCAGATATAAAACTGGCTGTACGCTCTATTTTCTTTGCAGCAGTCGGTACTGCTGGTCAGCGGTGCACAACCTGCCGTAGACTGGTATTAAAACCAATTTTGAAAACTTGAGTTCACAATTGTAAAGCATCCATGTTGTTTCCGTAAGGTATTTAATATTCAATTTTGGCTTTGCATGCAGTTTCTGCATGAGAGTATTTATGCAAATGTACTAGATCAACTTGCCGGACTTTACAAACAAGTCAAAATCGGGAATCCCCTGGAGAAAGGGACTCTTGTTGGGCCCCTGCATACTCGCTCTTCCGTGGAAAACTTCAAGAAGGGCATTGCGGCGATAAAATCTCAGGCATGTCATGTCAACTCTTTTCTAGTTAAATATGGTCAGCATGTAACTTCCCATGTGTTATGTCCAATTTTGTACGGTATTGTATCCAAATTTTGCTCAAATATGATGGGTGTGTATTCTTTGTGCCGAGATGCTTACTCGATACTCTGATTATCAGGGTGGGAAGATCCTAACAGGGGGGTCTGTAGTGGAGTCAGACGGAAATTTTGTACAGCCAACAATTGTTGAGATTTCTGCAGATGCTGCTGTTGTTAAAGAAGAACTGTTCGCTCCAGTTCTCTATGTTATGAAATTTCAGGTAGTATATTATCTAAATCTTTTTATTGAACTTATTTCCTTACAAATTTAGTTTCTGATTTCACTTCACGATTTTTCTATATAGAATCTCGAAGAAGCAATTGCCTTGAACAATTCTGTTCCTCAAGGACTGAGTAGTTCAATCTTTACACAAAGACCTGATACTATATTCAAATGGATCGGGTAACTATCTGGACTTCTTTTTTCGTCATTTCCTCTAGATACCTTCTAATGACTTCAGCTTAGGAACTTGTCTACTAACCAAAACCCCTTAAAAGATGTCTGTCGTGTTTTTTAGTATATAAGCAATATATTTTGTGTATTCATGAGTGTGATTGCCGTGCAGGCCAAGAGGGAGTGATTGTGGTATTGTAAATGTGAACATACCAACAAATGGAGCTGAGATTGGGGGAGCCTTTGGTGGAGAAAAGGCAACTGGTGGTGGTCGCGAAGCAGGGAGTGACTCGTGGAAGCAATACATGCGCCGTTCTACATGGCAAGTCTTAGTTGAATTTAAGATGTTGCATGTCCCTTACTTATAA

Protein sequence

>MS.gene64463.t1

MGSDQSNNLEFLKEIGLGSTNIGCFINGQWKANGSSVTSVNPSTNQTIATVTEATVIDYEEGLQACSEAAKTWMTIPAPKRGEIVRQIGDALRAKLDPLGRLVALEMGKILAEGIGEVQEIIDMCDYAVGLSRQLNGSIIPSERPDHMMFEVWNPLGIVGVITAFNFPCAVLGWNACIALVCGNCVVWKGAPTTPLITIAITKLISEVIERNKLPGAIFTSFCGGADIGQAISKDRRIPLVSFTGSSKVGAEVQKTVNERFGKCLLELSGNNAIIVMDDADIKLAVRSIFFAAVGTAGQRCTTCRRLFLHESIYANVLDQLAGLYKQVKIGNPLEKGTLVGPLHTRSSVENFKKGIAAIKSQGGKILTGGSVVESDGNFVQPTIVEISADAAVVKEELFAPVLYVMKFQNLEEAIALNNSVPQGLSSSIFTQRPDTIFKWIGPRGSDCGIVNVNIPTNGAEIGGAFGGEKATGGGREAGSDSWKQYMRRSTWQVLVEFKMLHVPYL