AlfalfaGEDB | MS.gene071481

M. sativa cultivar XinJiangDaYe / MS.gene071481

KEGG: K05752
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071481.t1	XP_003517200.1	100	85	0	0	1	85	1	85	2.20E-37	164.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071481.t1	Q94JY4	90.6	85	8	0	1	85	1	85	1.6e-36	152.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071481.t1	A0A0B2PBB9	100.0	85	0	0	1	85	1	85	1.6e-37	164.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene071481	0.826737	2.30E-54	-1.69E-46
MS.gene049255	MS.gene071481	0.800747	1.25E-48	-1.69E-46
MS.gene049341	MS.gene071481	0.818268	2.14E-52	-1.69E-46
MS.gene049821	MS.gene071481	0.809283	2.04E-50	-1.69E-46
MS.gene049850	MS.gene071481	0.819529	1.11E-52	-1.69E-46
MS.gene050020	MS.gene071481	0.848474	5.99E-60	-1.69E-46
MS.gene050303	MS.gene071481	0.809819	1.57E-50	-1.69E-46
MS.gene050355	MS.gene071481	0.80278	4.77E-49	-1.69E-46
MS.gene050442	MS.gene071481	0.82108	4.88E-53	-1.69E-46
MS.gene050724	MS.gene071481	0.804464	2.13E-49	-1.69E-46
MS.gene050807	MS.gene071481	0.814478	1.51E-51	-1.69E-46
MS.gene050815	MS.gene071481	0.857072	2.09E-62	-1.69E-46
MS.gene050844	MS.gene071481	0.800684	1.28E-48	-1.69E-46
MS.gene051247	MS.gene071481	0.809784	1.59E-50	-1.69E-46
MS.gene051328	MS.gene071481	0.816523	5.29E-52	-1.69E-46
MS.gene05157	MS.gene071481	0.818914	1.53E-52	-1.69E-46
MS.gene051719	MS.gene071481	0.823931	1.06E-53	-1.69E-46
MS.gene051720	MS.gene071481	0.806722	7.17E-50	-1.69E-46
MS.gene051744	MS.gene071481	0.808206	3.47E-50	-1.69E-46
MS.gene052012	MS.gene071481	0.81057	1.08E-50	-1.69E-46
MS.gene052020	MS.gene071481	0.805991	1.02E-49	-1.69E-46
MS.gene052519	MS.gene071481	0.835142	1.99E-56	-1.69E-46
MS.gene052584	MS.gene071481	0.803963	2.71E-49	-1.69E-46
MS.gene052611	MS.gene071481	0.845609	3.65E-59	-1.69E-46
MS.gene052863	MS.gene071481	0.828876	7.05E-55	-1.69E-46
MS.gene052896	MS.gene071481	0.800211	1.60E-48	-1.69E-46
MS.gene053008	MS.gene071481	0.827572	1.45E-54	-1.69E-46
MS.gene053226	MS.gene071481	0.836188	1.08E-56	-1.69E-46
MS.gene054236	MS.gene071481	0.809621	1.73E-50	-1.69E-46
MS.gene05462	MS.gene071481	0.801461	8.91E-49	-1.69E-46
MS.gene054658	MS.gene071481	0.804338	2.27E-49	-1.69E-46
MS.gene054775	MS.gene071481	0.814741	1.32E-51	-1.69E-46
MS.gene055166	MS.gene071481	0.815398	9.44E-52	-1.69E-46
MS.gene055358	MS.gene071481	0.80014	1.66E-48	-1.69E-46
MS.gene055465	MS.gene071481	0.812782	3.57E-51	-1.69E-46
MS.gene055466	MS.gene071481	0.846659	1.89E-59	-1.69E-46
MS.gene055468	MS.gene071481	0.820043	8.45E-53	-1.69E-46
MS.gene055778	MS.gene071481	0.827566	1.46E-54	-1.69E-46
MS.gene055818	MS.gene071481	0.844631	6.71E-59	-1.69E-46
MS.gene05604	MS.gene071481	0.802665	5.04E-49	-1.69E-46
MS.gene056162	MS.gene071481	0.805574	1.25E-49	-1.69E-46
MS.gene056215	MS.gene071481	0.827399	1.60E-54	-1.69E-46
MS.gene056498	MS.gene071481	0.805064	1.60E-49	-1.69E-46
MS.gene056595	MS.gene071481	0.817825	2.70E-52	-1.69E-46
MS.gene057060	MS.gene071481	0.840991	6.23E-58	-1.69E-46
MS.gene057314	MS.gene071481	0.802753	4.83E-49	-1.69E-46
MS.gene057444	MS.gene071481	0.803378	3.59E-49	-1.69E-46
MS.gene057693	MS.gene071481	0.849683	2.76E-60	-1.69E-46
MS.gene057915	MS.gene071481	0.81059	1.07E-50	-1.69E-46
MS.gene058084	MS.gene071481	0.81069	1.02E-50	-1.69E-46
MS.gene058168	MS.gene071481	0.803005	4.29E-49	-1.69E-46
MS.gene058625	MS.gene071481	0.804645	1.96E-49	-1.69E-46
MS.gene059151	MS.gene071481	0.833116	6.42E-56	-1.69E-46
MS.gene05954	MS.gene071481	0.816521	5.30E-52	-1.69E-46
MS.gene059842	MS.gene071481	0.812739	3.64E-51	-1.69E-46
MS.gene060322	MS.gene071481	0.825525	4.47E-54	-1.69E-46
MS.gene060429	MS.gene071481	0.849329	3.47E-60	-1.69E-46
MS.gene060553	MS.gene071481	0.807785	4.27E-50	-1.69E-46
MS.gene060572	MS.gene071481	0.821577	3.75E-53	-1.69E-46
MS.gene060781	MS.gene071481	0.802942	4.41E-49	-1.69E-46
MS.gene060803	MS.gene071481	0.849476	3.16E-60	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071481.t1	MTR_5g030935	100.000	85	0	0	1	85	1	85	2.67e-57	171

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071481.t1	AT2G22640	90.588	85	8	0	1	85	1	85	6.83e-53	159

Find 12 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGAAATTCAGTACCGAAT+TGG	0.368507	5.2:+20923603	None:intergenic
TGATGTATTGGAGCGTCGTT+TGG	0.385920	5.2:-20923446	MS.gene071481:CDS
GAATCGCTGTTCAAGCTGAT+TGG	0.386107	5.2:-20923688	MS.gene071481:CDS
TTCGATTTCCTTGTCCAATT+CGG	0.396293	5.2:-20923617	MS.gene071481:intron
GAATGAAAAGCTTGATGTAT+TGG	0.415813	5.2:-20923458	MS.gene071481:CDS
AATCGCTGTTCAAGCTGATT+GGG	0.456401	5.2:-20923687	MS.gene071481:CDS
AGTTCAAGTTGGAAATGCTT+CGG	0.460394	5.2:-20923413	MS.gene071481:CDS
GAATTACTTGAAGTTCAAGT+TGG	0.488446	5.2:-20923424	MS.gene071481:CDS
ATTCCATCAATGGCACGTGC+TGG	0.545650	5.2:-20923737	None:intergenic
CCATCAATGGCACGTGCTGG+TGG	0.566134	5.2:-20923734	None:intergenic
ATTCAGTACCGAATTGGACA+AGG	0.614288	5.2:+20923609	None:intergenic
ATCACAAACGCAGTTAACGT+TGG	0.620945	5.2:-20923710	MS.gene071481:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATAAGATTTCAAATAACA+AGG	+	chr5.2:20923568-20923587	None:intergenic	15.0%
!!!	AATCTTATATCTTTTTTTGA+TGG	-	chr5.2:20923578-20923597	MS.gene071481:intron	15.0%
!!!	ATCTTATATCTTTTTTTGAT+GGG	-	chr5.2:20923579-20923598	MS.gene071481:intron	15.0%
!!!	TGTTATTTTTGTTATTTCAG+AGG	-	chr5.2:20923606-20923625	MS.gene071481:intron	20.0%
!	ATTTCAAATAACAAGGCAAA+AGG	+	chr5.2:20923561-20923580	None:intergenic	25.0%
!	TTTCAAATAACAAGGCAAAA+GGG	+	chr5.2:20923560-20923579	None:intergenic	25.0%
	GAATGAAAAGCTTGATGTAT+TGG	-	chr5.2:20923657-20923676	MS.gene071481:CDS	30.0%
	GAATTACTTGAAGTTCAAGT+TGG	-	chr5.2:20923691-20923710	MS.gene071481:CDS	30.0%
	TTCGATTTCCTTGTCCAATT+CGG	-	chr5.2:20923498-20923517	MS.gene071481:intron	35.0%
!	GAAGAAATTCAGTACCGAAT+TGG	+	chr5.2:20923515-20923534	None:intergenic	35.0%
!!	AGTTCAAGTTGGAAATGCTT+CGG	-	chr5.2:20923702-20923721	MS.gene071481:CDS	35.0%
	AATCGCTGTTCAAGCTGATT+GGG	-	chr5.2:20923428-20923447	MS.gene071481:CDS	40.0%
	ATCACAAACGCAGTTAACGT+TGG	-	chr5.2:20923405-20923424	MS.gene071481:CDS	40.0%
!	ATTCAGTACCGAATTGGACA+AGG	+	chr5.2:20923509-20923528	None:intergenic	40.0%
	GAATCGCTGTTCAAGCTGAT+TGG	-	chr5.2:20923427-20923446	MS.gene071481:CDS	45.0%
!!	TGATGTATTGGAGCGTCGTT+TGG	-	chr5.2:20923669-20923688	MS.gene071481:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	20923387	20923750	20923387	ID=MS.gene071481
chr5.2	mRNA	20923387	20923750	20923387	ID=MS.gene071481.t1;Parent=MS.gene071481
chr5.2	exon	20923618	20923750	20923618	ID=MS.gene071481.t1.exon1;Parent=MS.gene071481.t1
chr5.2	CDS	20923618	20923750	20923618	ID=cds.MS.gene071481.t1;Parent=MS.gene071481.t1
chr5.2	exon	20923387	20923511	20923387	ID=MS.gene071481.t1.exon2;Parent=MS.gene071481.t1
chr5.2	CDS	20923387	20923511	20923387	ID=cds.MS.gene071481.t1;Parent=MS.gene071481.t1

Gene Sequence	>MS.gene071481 ATGGCACGTGCTGGTGGAATCACAAACGCAGTTAACGTTGGAATCGCTGTTCAAGCTGATTGGGAAAATCGTGAATTCATCTCTCACATTTCTCTCAATGTTCGTCGTCTCTTCGATTTCCTTGTCCAATTCGGTACTGAATTTCTTCTCAATTTTCTGTTTTTCTTTGACCCTTTTGCCTTGTTATTTGAAATCTTATATCTTTTTTTGATGGGTTGTTGTTATTTTTGTTATTTCAGAGGCTACAACAAAGAGTAAATTAGCTTCATTGAATGAAAAGCTTGATGTATTGGAGCGTCGTTTGGAATTACTTGAAGTTCAAGTTGGAAATGCTTCGGCAAATCCTTCTCTTTTTGCAACTTGA
Protein sequence	>MS.gene071481.t1 MARAGGITNAVNVGIAVQADWENREFISHISLNVRRLFDFLVQFEATTKSKLASLNEKLDVLERRLELLEVQVGNASANPSLFAT