AlfalfaGEDB | MS.gene072300

M. sativa cultivar XinJiangDaYe / MS.gene072300

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072300.t1	XP_003626832.1	97.1	35	1	0	38	72	1	35	2.80E-09	70.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072300.t1	G7LDH4	97.1	35	1	0	38	72	1	35	2.0e-09	70.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene072300	0.840582	7.97E-58	-1.69E-46
MS.gene049254	MS.gene072300	0.814174	1.76E-51	-1.69E-46
MS.gene049435	MS.gene072300	0.812733	3.65E-51	-1.69E-46
MS.gene049585	MS.gene072300	0.83234	9.99E-56	-1.69E-46
MS.gene049821	MS.gene072300	0.830463	2.90E-55	-1.69E-46
MS.gene050442	MS.gene072300	0.818867	1.57E-52	-1.69E-46
MS.gene050807	MS.gene072300	0.8313	1.80E-55	-1.69E-46
MS.gene050808	MS.gene072300	0.801966	7.02E-49	-1.69E-46
MS.gene050826	MS.gene072300	0.822989	1.76E-53	-1.69E-46
MS.gene051198	MS.gene072300	0.834143	3.55E-56	-1.69E-46
MS.gene051247	MS.gene072300	0.823141	1.63E-53	-1.69E-46
MS.gene051279	MS.gene072300	0.803691	3.09E-49	-1.69E-46
MS.gene051700	MS.gene072300	0.804327	2.28E-49	-1.69E-46
MS.gene051719	MS.gene072300	0.80784	4.15E-50	-1.69E-46
MS.gene051720	MS.gene072300	0.818825	1.60E-52	-1.69E-46
MS.gene051744	MS.gene072300	0.818229	2.19E-52	-1.69E-46
MS.gene051800	MS.gene072300	0.818823	1.60E-52	-1.69E-46
MS.gene052168	MS.gene072300	0.804804	1.81E-49	-1.69E-46
MS.gene052239	MS.gene072300	-0.809582	1.76E-50	-1.69E-46
MS.gene052516	MS.gene072300	0.802146	6.44E-49	-1.69E-46
MS.gene052582	MS.gene072300	0.816544	5.24E-52	-1.69E-46
MS.gene052584	MS.gene072300	0.809188	2.14E-50	-1.69E-46
MS.gene052609	MS.gene072300	0.801938	7.11E-49	-1.69E-46
MS.gene052673	MS.gene072300	-0.807616	4.63E-50	-1.69E-46
MS.gene052773	MS.gene072300	0.824691	7.04E-54	-1.69E-46
MS.gene053226	MS.gene072300	0.81132	7.43E-51	-1.69E-46
MS.gene05414	MS.gene072300	0.806646	7.44E-50	-1.69E-46
MS.gene05415	MS.gene072300	0.836212	1.07E-56	-1.69E-46
MS.gene054291	MS.gene072300	-0.80045	1.43E-48	-1.69E-46
MS.gene054467	MS.gene072300	0.803366	3.61E-49	-1.69E-46
MS.gene054576	MS.gene072300	0.835688	1.45E-56	-1.69E-46
MS.gene054775	MS.gene072300	0.812444	4.23E-51	-1.69E-46
MS.gene054991	MS.gene072300	0.800007	1.76E-48	-1.69E-46
MS.gene055465	MS.gene072300	0.83611	1.13E-56	-1.69E-46
MS.gene055466	MS.gene072300	0.8204	7.00E-53	-1.69E-46
MS.gene055468	MS.gene072300	0.816967	4.21E-52	-1.69E-46
MS.gene055469	MS.gene072300	0.82046	6.78E-53	-1.69E-46
MS.gene055778	MS.gene072300	0.802933	4.44E-49	-1.69E-46
MS.gene055818	MS.gene072300	0.822168	2.74E-53	-1.69E-46
MS.gene055926	MS.gene072300	0.836829	7.44E-57	-1.69E-46
MS.gene05604	MS.gene072300	0.82911	6.18E-55	-1.69E-46
MS.gene056174	MS.gene072300	0.841238	5.36E-58	-1.69E-46
MS.gene056308	MS.gene072300	0.827507	1.51E-54	-1.69E-46
MS.gene056507	MS.gene072300	0.8031	4.09E-49	-1.69E-46
MS.gene057026	MS.gene072300	0.8053	1.43E-49	-1.69E-46
MS.gene057444	MS.gene072300	0.809364	1.96E-50	-1.69E-46
MS.gene057598	MS.gene072300	0.834443	2.99E-56	-1.69E-46
MS.gene057869	MS.gene072300	0.808006	3.83E-50	-1.69E-46
MS.gene057915	MS.gene072300	0.821872	3.20E-53	-1.69E-46
MS.gene058010	MS.gene072300	0.825393	4.80E-54	-1.69E-46
MS.gene05847	MS.gene072300	0.820605	6.28E-53	-1.69E-46
MS.gene058979	MS.gene072300	0.804718	1.89E-49	-1.69E-46
MS.gene05897	MS.gene072300	0.801328	9.48E-49	-1.69E-46
MS.gene059439	MS.gene072300	0.839005	2.05E-57	-1.69E-46
MS.gene059440	MS.gene072300	0.810318	1.22E-50	-1.69E-46
MS.gene059584	MS.gene072300	0.811367	7.25E-51	-1.69E-46
MS.gene059625	MS.gene072300	0.80609	9.74E-50	-1.69E-46
MS.gene059725	MS.gene072300	0.813284	2.77E-51	-1.69E-46
MS.gene060167	MS.gene072300	0.808247	3.40E-50	-1.69E-46
MS.gene060373	MS.gene072300	0.801408	9.13E-49	-1.69E-46
MS.gene060521	MS.gene072300	0.805226	1.48E-49	-1.69E-46
MS.gene06052	MS.gene072300	0.831826	1.34E-55	-1.69E-46
MS.gene060634	MS.gene072300	-0.823317	1.48E-53	-1.69E-46
MS.gene060918	MS.gene072300	0.806738	7.11E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072300.t1	MTR_8g009530	97.143	35	1	0	38	72	1	35	1.31e-16	72.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 8 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGTGGAGATTCAGGTTTAT+TGG	0.277049	8.3:+75818895	MS.gene072300:CDS
CCACAGAAAAGCAATGTTAA+AGG	0.278986	8.3:+75818853	MS.gene072300:CDS
CTTAGTTTGGTCTAGTTTCA+TGG	0.285048	8.3:-75818307	None:intergenic
CACAGAAAAGCAATGTTAAA+GGG	0.424518	8.3:+75818854	MS.gene072300:CDS
TATGTTTCAAGCTTCAATGT+AGG	0.493248	8.3:-75818950	None:intergenic
CATGAAACTAGACCAAACTA+AGG	0.496906	8.3:+75818308	None:intergenic
TCTACAGCTCAAATCATCAC+TGG	0.580533	8.3:+75818700	MS.gene072300:CDS
ATGAAACTAGACCAAACTAA+GGG	0.653009	8.3:+75818309	MS.gene072300:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCAAACAATAAATAAATTTT+AGG	-	chr8.3:75818584-75818603	None:intergenic	10.0%
!!	AATACACAAATTTAATTGAT+TGG	+	chr8.3:75818521-75818540	MS.gene072300:intron	15.0%
!!!	TAGAAAATAATAGTGTATTT+TGG	-	chr8.3:75818348-75818367	None:intergenic	15.0%
!!!	AATGAAAAATTGGTTTGAAA+GGG	-	chr8.3:75818755-75818774	None:intergenic	20.0%
!	ACGATGTCATTATAAATGTA+CGG	-	chr8.3:75818389-75818408	None:intergenic	25.0%
!	ATTGATTGGCATTGTAAAAA+TGG	+	chr8.3:75818535-75818554	MS.gene072300:intron	25.0%
!	GTCAAGAAAAAAATATGACA+TGG	+	chr8.3:75818495-75818514	MS.gene072300:intron	25.0%
!	GTGAAATTGGAATGAAAAAT+TGG	-	chr8.3:75818765-75818784	None:intergenic	25.0%
!	TTCTACAAAAAGCGATTAAT+GGG	-	chr8.3:75818830-75818849	None:intergenic	25.0%
!	TTTCTACAAAAAGCGATTAA+TGG	-	chr8.3:75818831-75818850	None:intergenic	25.0%
!!	TTTTAGATTTACCCTTAGTT+TGG	-	chr8.3:75818323-75818342	None:intergenic	25.0%
!!!	AAGGGAAAATTATCGTTTTT+CGG	+	chr8.3:75818872-75818891	MS.gene072300:CDS	25.0%
!!!	GAATGAAAAATTGGTTTGAA+AGG	-	chr8.3:75818756-75818775	None:intergenic	25.0%
!!!	TTATCTAAAGCGATATTTTG+AGG	-	chr8.3:75818449-75818468	None:intergenic	25.0%
	ATGAAACTAGACCAAACTAA+GGG	+	chr8.3:75818309-75818328	MS.gene072300:CDS	30.0%
	CACAGAAAAGCAATGTTAAA+GGG	+	chr8.3:75818854-75818873	MS.gene072300:CDS	30.0%
	CGATGTCATTATAAATGTAC+GGG	-	chr8.3:75818388-75818407	None:intergenic	30.0%
	TATGTTTCAAGCTTCAATGT+AGG	-	chr8.3:75818953-75818972	None:intergenic	30.0%
	CCACAGAAAAGCAATGTTAA+AGG	+	chr8.3:75818853-75818872	MS.gene072300:CDS	35.0%
	GTCGTGAATGAATGTGAAAT+TGG	-	chr8.3:75818778-75818797	None:intergenic	35.0%
	TGAAAGGGAAATTGAAGGAA+TGG	-	chr8.3:75818740-75818759	None:intergenic	35.0%
	TGGTTTGAAAGGGAAATTGA+AGG	-	chr8.3:75818745-75818764	None:intergenic	35.0%
!	GAGTGTTCGTTCTTTTCAAA+CGG	-	chr8.3:75818682-75818701	None:intergenic	35.0%
!!	AAATTATCGTTTTTCGGAGG+TGG	+	chr8.3:75818878-75818897	MS.gene072300:CDS	35.0%
!!	CCTTTAACATTGCTTTTCTG+TGG	-	chr8.3:75818856-75818875	None:intergenic	35.0%
!!	GGAAAATTATCGTTTTTCGG+AGG	+	chr8.3:75818875-75818894	MS.gene072300:CDS	35.0%
	TCTACAGCTCAAATCATCAC+TGG	+	chr8.3:75818700-75818719	MS.gene072300:CDS	40.0%
!	AGGTGGAGATTCAGGTTTAT+TGG	+	chr8.3:75818895-75818914	MS.gene072300:CDS	40.0%
!!	TTTTTCGGAGGTGGAGATTC+AGG	+	chr8.3:75818887-75818906	MS.gene072300:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	75818309	75818973	75818309	ID=MS.gene072300
chr8.3	mRNA	75818309	75818973	75818309	ID=MS.gene072300.t1;Parent=MS.gene072300
chr8.3	exon	75818309	75818330	75818309	ID=MS.gene072300.t1.exon1;Parent=MS.gene072300.t1
chr8.3	CDS	75818309	75818330	75818309	ID=cds.MS.gene072300.t1;Parent=MS.gene072300.t1
chr8.3	exon	75818651	75818721	75818651	ID=MS.gene072300.t1.exon2;Parent=MS.gene072300.t1
chr8.3	CDS	75818651	75818721	75818651	ID=cds.MS.gene072300.t1;Parent=MS.gene072300.t1
chr8.3	exon	75818848	75818973	75818848	ID=MS.gene072300.t1.exon3;Parent=MS.gene072300.t1
chr8.3	CDS	75818848	75818973	75818848	ID=cds.MS.gene072300.t1;Parent=MS.gene072300.t1

Gene Sequence

>MS.gene072300

ATGAAACTAGACCAAACTAAGGGTAAATCTAAAATACCAAAATACACTATTATTTTCTACTAATTTTTTATTAATACCCGTACATTTATAATGACATCGTAGTAAATTTACTTTATTTTGTTTGTGTTATTTGTTGTCCTCAAAATATCGCTTTAGATAAAAAGTTGTTCAATACTTTAAAATTATGTCAAGAAAAAAATATGACATGGCAAAATACACAAATTTAATTGATTGGCATTGTAAAAATGGTTTTACACTGTCAGCGCATCTATCCTAAAATTTATTTATTGTTTGAAATTGAAACATTCAATATCAATACTTACATCATTCAATTGTGAATAGTTGATTTTTTCTACGACTCAGATCAGATCCGTTTGAAAAGAACGAACACTCTACAGCTCAAATCATCACTGGTAAATTCTTCAATTCCATTCCTTCAATTTCCCTTTCAAACCAATTTTTCATTCCAATTTCACATTCATTCACGACAACATTTCAAAATCGCTTATTTAAGCTTACCCATTAATCGCTTTTTGTAGAAAATCCACAGAAAAGCAATGTTAAAGGGAAAATTATCGTTTTTCGGAGGTGGAGATTCAGGTTTATTGGATAAAAATTCATCAAAGAATGAAGAGATTGTTCCTACATTGAAGCTTGAAACATAG

Protein sequence

>MS.gene072300.t1

MKLDQTKVDFFYDSDQIRLKRTNTLQLKSSLKIHRKAMLKGKLSFFGGGDSGLLDKNSSKNEEIVPTLKLET