AlfalfaGEDB | MS.gene09580

M. sativa cultivar XinJiangDaYe / MS.gene09580

KEGG: K17428
GO: GO:0000313 GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005761 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF06984.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09580.t1	XP_003611613.2	97.8	137	2	1	1	136	1	137	7.30E-67	263.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09580.t1	Q8K2Y7	50.6	89	43	1	29	116	63	151	2.1e-14	80.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09580.t1	G7K3V1	97.8	137	2	1	1	136	1	137	5.2e-67	263.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene09580	0.840829	6.87E-58	-1.69E-46
MS.gene050277	MS.gene09580	0.844897	5.69E-59	-1.69E-46
MS.gene050303	MS.gene09580	0.80556	1.26E-49	-1.69E-46
MS.gene050752	MS.gene09580	0.802018	6.84E-49	-1.69E-46
MS.gene05134	MS.gene09580	0.838434	2.88E-57	-1.69E-46
MS.gene051746	MS.gene09580	0.824142	9.47E-54	-1.69E-46
MS.gene051748	MS.gene09580	0.817704	2.87E-52	-1.69E-46
MS.gene05188	MS.gene09580	0.809405	1.92E-50	-1.69E-46
MS.gene051903	MS.gene09580	0.810032	1.41E-50	-1.69E-46
MS.gene052947	MS.gene09580	0.838481	2.80E-57	-1.69E-46
MS.gene053109	MS.gene09580	0.816936	4.28E-52	-1.69E-46
MS.gene054403	MS.gene09580	0.802158	6.41E-49	-1.69E-46
MS.gene056631	MS.gene09580	0.864274	1.37E-64	-1.69E-46
MS.gene056986	MS.gene09580	0.802527	5.38E-49	-1.69E-46
MS.gene057140	MS.gene09580	0.822833	1.92E-53	-1.69E-46
MS.gene057705	MS.gene09580	0.824645	7.22E-54	-1.69E-46
MS.gene058906	MS.gene09580	0.827001	1.99E-54	-1.69E-46
MS.gene059898	MS.gene09580	0.829273	5.64E-55	-1.69E-46
MS.gene06001	MS.gene09580	0.84062	7.79E-58	-1.69E-46
MS.gene060429	MS.gene09580	0.82936	5.38E-55	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene09580	MS.gene005865	PPI
MS.gene09580	MS.gene21227	PPI
MS.gene31468	MS.gene09580	PPI
MS.gene005399	MS.gene09580	PPI
MS.gene09580	MS.gene035256	PPI
MS.gene09580	MS.gene20938	PPI
MS.gene09580	MS.gene028903	PPI
MS.gene09580	MS.gene055808	PPI
MS.gene34167	MS.gene09580	PPI
MS.gene09580	MS.gene82915	PPI
MS.gene09580	MS.gene043435	PPI
MS.gene21111	MS.gene09580	PPI
MS.gene09580	MS.gene001687	PPI
MS.gene09580	MS.gene066059	PPI
MS.gene09580	MS.gene21110	PPI
MS.gene60589	MS.gene09580	PPI
MS.gene09580	MS.gene036564	PPI
MS.gene09580	MS.gene026968	PPI
MS.gene09580	MS.gene60861	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09580.t1	MTR_5g015850	97.810	137	2	1	1	136	1	137	6.36e-97	275

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09580.t1	AT1G07830	71.528	144	33	3	1	136	1	144	4.39e-67	199

Find 25 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGAATTGCGTCTGAAATCT+TGG	0.295639	5.2:-7560125	MS.gene09580:CDS
CCCTCTTCCGGAGTTCTTTG+AGG	0.397007	5.2:-7560340	MS.gene09580:CDS
GAGATGAAGAAAATGATAAA+TGG	0.401349	5.2:-7559668	MS.gene09580:CDS
TTAGGTAAGCGCTCCGGGTT+AGG	0.401390	5.2:+7560009	None:intergenic
GTTGTGGTATGTGTTGTTAA+AGG	0.420053	5.2:-7560088	MS.gene09580:CDS
AACGAGACACCAGTTGTCTA+TGG	0.421926	5.2:-7560306	MS.gene09580:intron
TGAATTGCGTCTGAAATCTT+GGG	0.436291	5.2:-7560124	MS.gene09580:CDS
TGCTGTTAGAAACCCTCTTC+CGG	0.440920	5.2:-7560352	MS.gene09580:CDS
TCAGCAGACCTCCTTGCGTC+TGG	0.463615	5.2:+7559689	None:intergenic
ATACCTTAGGTAAGCGCTCC+GGG	0.470522	5.2:+7560004	None:intergenic
GAACTTGCGCTTTCCTAACC+CGG	0.481233	5.2:-7560022	MS.gene09580:CDS
GCCTCAAAGAACTCCGGAAG+AGG	0.493349	5.2:+7560339	None:intergenic
TATTTCATACCATAGACAAC+TGG	0.503485	5.2:+7560297	None:intergenic
AATACCTTAGGTAAGCGCTC+CGG	0.514447	5.2:+7560003	None:intergenic
AAACAGTATTCCACTACAGC+TGG	0.543067	5.2:-7560375	MS.gene09580:CDS
ACACATACCACAACTTATGA+AGG	0.545630	5.2:+7560097	None:intergenic
TTTGTTAGGTCGGAGTTGGA+AGG	0.551769	5.2:-7560151	MS.gene09580:intron
GGGATGACCTTCATAAGTTG+TGG	0.556409	5.2:-7560104	MS.gene09580:CDS
TCTAACAGCACCAGCTGTAG+TGG	0.557909	5.2:+7560365	None:intergenic
TAACCCGGAGCGCTTACCTA+AGG	0.570629	5.2:-7560007	MS.gene09580:intron
CTGTCTGCCTCAAAGAACTC+CGG	0.578032	5.2:+7560333	None:intergenic
CCTCAAAGAACTCCGGAAGA+GGG	0.599992	5.2:+7560340	None:intergenic
CGATTGAAGAACCAGACGCA+AGG	0.611981	5.2:-7559700	MS.gene09580:CDS
CAGTAAGCACATGCTTGATG+CGG	0.642610	5.2:+7559729	None:intergenic
TTGAAGAACCAGACGCAAGG+AGG	0.691763	5.2:-7559697	MS.gene09580:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAATGTTAAATTTCGTAT+GGG	-	chr5.2:7560260-7560279	MS.gene09580:intron	15.0%
!!	GTAAAATGTTAAATTTCGTA+TGG	-	chr5.2:7560259-7560278	MS.gene09580:intron	20.0%
!!!	ATAGTTTTACCATTTTTGTT+AGG	-	chr5.2:7559925-7559944	MS.gene09580:intron	20.0%
!!!	GATTTAATTTATGTGAAATC+AGG	-	chr5.2:7560322-7560341	MS.gene09580:CDS	20.0%
!	AAAAAGTGAAGAAATACCTT+AGG	+	chr5.2:7560102-7560121	None:intergenic	25.0%
!	GAGATGAAGAAAATGATAAA+TGG	-	chr5.2:7560422-7560441	MS.gene09580:CDS	25.0%
!	TATAAGAAAACAAAGTGATG+AGG	+	chr5.2:7559908-7559927	None:intergenic	25.0%
!!!	ATTTTTTTGTACCTAATGTG+TGG	+	chr5.2:7559876-7559895	None:intergenic	25.0%
!!!	TTTTACCATTTTTGTTAGGT+CGG	-	chr5.2:7559929-7559948	MS.gene09580:intron	25.0%
	AGTTATGTGGACATTGATAT+AGG	-	chr5.2:7560162-7560181	MS.gene09580:intron	30.0%
	ATCAATGTCCACATAACTAA+GGG	+	chr5.2:7560160-7560179	None:intergenic	30.0%
	ATGTTAAATTTCGTATGGGT+TGG	-	chr5.2:7560264-7560283	MS.gene09580:intron	30.0%
	CCTATTCTATTGAAAAGAAG+AGG	+	chr5.2:7559823-7559842	None:intergenic	30.0%
	TATCAATGTCCACATAACTA+AGG	+	chr5.2:7560161-7560180	None:intergenic	30.0%
	TATTTCATACCATAGACAAC+TGG	+	chr5.2:7559796-7559815	None:intergenic	30.0%
	TGTTAAATTTCGTATGGGTT+GGG	-	chr5.2:7560265-7560284	MS.gene09580:intron	30.0%
	TTTCATTATAGCCACACATT+AGG	-	chr5.2:7559862-7559881	MS.gene09580:intron	30.0%
!	CCTCTTCTTTTCAATAGAAT+AGG	-	chr5.2:7559820-7559839	MS.gene09580:intron	30.0%
!	TTTTGTTACCCTTAGTTATG+TGG	-	chr5.2:7560149-7560168	MS.gene09580:intron	30.0%
!!!	ATTTCTTCACTTTTTACACC+TGG	-	chr5.2:7560107-7560126	MS.gene09580:CDS	30.0%
	ACACATACCACAACTTATGA+AGG	+	chr5.2:7559996-7560015	None:intergenic	35.0%
	ATTGATATAGGAAATCGCAG+TGG	-	chr5.2:7560174-7560193	MS.gene09580:intron	35.0%
!	GTTAAATTTCGTATGGGTTG+GGG	-	chr5.2:7560266-7560285	MS.gene09580:intron	35.0%
!	GTTGTGGTATGTGTTGTTAA+AGG	-	chr5.2:7560002-7560021	MS.gene09580:intron	35.0%
!	TGAATTGCGTCTGAAATCTT+GGG	-	chr5.2:7559966-7559985	MS.gene09580:intron	35.0%
!!	TTAAATTTCGTATGGGTTGG+GGG	-	chr5.2:7560267-7560286	MS.gene09580:intron	35.0%
!!!	AGTGGAATACTGTTTTGATC+TGG	+	chr5.2:7559710-7559729	None:intergenic	35.0%
	AAACAGTATTCCACTACAGC+TGG	-	chr5.2:7559715-7559734	MS.gene09580:CDS	40.0%
	CAACTCCGACCTAACAAAAA+TGG	+	chr5.2:7559937-7559956	None:intergenic	40.0%
!	CATTTTTGTTAGGTCGGAGT+TGG	-	chr5.2:7559935-7559954	MS.gene09580:intron	40.0%
!	CTGAATTGCGTCTGAAATCT+TGG	-	chr5.2:7559965-7559984	MS.gene09580:intron	40.0%
!	GTGGTGCTTTTATCAACGTT+TGG	-	chr5.2:7560196-7560215	MS.gene09580:intron	40.0%
!!	TAAATTTCGTATGGGTTGGG+GGG	-	chr5.2:7560268-7560287	MS.gene09580:intron	40.0%
	AACGAGACACCAGTTGTCTA+TGG	-	chr5.2:7559784-7559803	MS.gene09580:intron	45.0%
	AATACCTTAGGTAAGCGCTC+CGG	+	chr5.2:7560090-7560109	None:intergenic	45.0%
	CAGTAAGCACATGCTTGATG+CGG	+	chr5.2:7560364-7560383	None:intergenic	45.0%
	GATATAGGAAATCGCAGTGG+TGG	-	chr5.2:7560177-7560196	MS.gene09580:intron	45.0%
	GGGATGACCTTCATAAGTTG+TGG	-	chr5.2:7559986-7560005	MS.gene09580:intron	45.0%
	TGCTGTTAGAAACCCTCTTC+CGG	-	chr5.2:7559738-7559757	MS.gene09580:CDS	45.0%
	TTTGTTAGGTCGGAGTTGGA+AGG	-	chr5.2:7559939-7559958	MS.gene09580:intron	45.0%
	ATACCTTAGGTAAGCGCTCC+GGG	+	chr5.2:7560089-7560108	None:intergenic	50.0%
	CAACACGCGTAAATCAGACC+AGG	+	chr5.2:7560128-7560147	None:intergenic	50.0%
	CCTCAAAGAACTCCGGAAGA+GGG	+	chr5.2:7559753-7559772	None:intergenic	50.0%
	CGATTGAAGAACCAGACGCA+AGG	-	chr5.2:7560390-7560409	MS.gene09580:CDS	50.0%
	CTGTCTGCCTCAAAGAACTC+CGG	+	chr5.2:7559760-7559779	None:intergenic	50.0%
	GAACTTGCGCTTTCCTAACC+CGG	-	chr5.2:7560068-7560087	MS.gene09580:CDS	50.0%
	TCTAACAGCACCAGCTGTAG+TGG	+	chr5.2:7559728-7559747	None:intergenic	50.0%
	TTGAAGAACCAGACGCAAGG+AGG	-	chr5.2:7560393-7560412	MS.gene09580:CDS	50.0%
	CCCTCTTCCGGAGTTCTTTG+AGG	-	chr5.2:7559750-7559769	MS.gene09580:intron	55.0%
	GCCTCAAAGAACTCCGGAAG+AGG	+	chr5.2:7559754-7559773	None:intergenic	55.0%
	GGTTGGGGGGAAACTGTTCA+CGG	-	chr5.2:7560281-7560300	MS.gene09580:intron	55.0%
	TTAGGTAAGCGCTCCGGGTT+AGG	+	chr5.2:7560084-7560103	None:intergenic	55.0%
!	TAACCCGGAGCGCTTACCTA+AGG	-	chr5.2:7560083-7560102	MS.gene09580:CDS	55.0%
	TCAGCAGACCTCCTTGCGTC+TGG	+	chr5.2:7560404-7560423	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	7559661	7560451	7559661	ID=MS.gene09580
chr5.2	mRNA	7559661	7560451	7559661	ID=MS.gene09580.t1;Parent=MS.gene09580
chr5.2	exon	7560307	7560451	7560307	ID=MS.gene09580.t1.exon1;Parent=MS.gene09580.t1
chr5.2	CDS	7560307	7560451	7560307	ID=cds.MS.gene09580.t1;Parent=MS.gene09580.t1
chr5.2	exon	7560008	7560165	7560008	ID=MS.gene09580.t1.exon2;Parent=MS.gene09580.t1
chr5.2	CDS	7560008	7560165	7560008	ID=cds.MS.gene09580.t1;Parent=MS.gene09580.t1
chr5.2	exon	7559661	7559768	7559661	ID=MS.gene09580.t1.exon3;Parent=MS.gene09580.t1
chr5.2	CDS	7559661	7559768	7559661	ID=cds.MS.gene09580.t1;Parent=MS.gene09580.t1

Gene Sequence

>MS.gene09580

ATGTTTTTGGCAAGAGCATTTCGACAATCATTCTTTGCTGCTGCTGCCAGATCAAAACAGTATTCCACTACAGCTGGTGCTGTTAGAAACCCTCTTCCGGAGTTCTTTGAGGCAGACAGAGATAACGAGACACCAGTTGTCTATGGTATGAAATATGCACCTCTTCTTTTCAATAGAATAGGAATTAAAAATCTCACTGATTTTCATTATAGCCACACATTAGGTACAAAAAAATTATAATTTTCCTCATCACTTTGTTTTCTTATAGTTTTACCATTTTTGTTAGGTCGGAGTTGGAAGGCTTCTGAATTGCGTCTGAAATCTTGGGATGACCTTCATAAGTTGTGGTATGTGTTGTTAAAGGAAAAGAATATGTTGATGACTCAACGACAAATGCTTCAAGCTCAGAACTTGCGCTTTCCTAACCCGGAGCGCTTACCTAAGGTATTTCTTCACTTTTTACACCTGGTCTGATTTACGCGTGTTGTTTTTGTTACCCTTAGTTATGTGGACATTGATATAGGAAATCGCAGTGGTGGTGCTTTTATCAACGTTTGGTGAACACTTTTTGTTTTAGAACATACAAGAGAAATTGAATGTAAAATGTTAAATTTCGTATGGGTTGGGGGGAAACTGTTCACGGTTAACAATGATTTTTGTTGATTTAATTTATGTGAAATCAGGTGAGAAAGTCAATGTGCCGCATCAAGCATGTGCTTACTGAGAGAGCGATTGAAGAACCAGACGCAAGGAGGTCTGCTGAGATGAAGAAAATGATAAATGGTCTTTGA

Protein sequence

>MS.gene09580.t1

MFLARAFRQSFFAAAARSKQYSTTAGAVRNPLPEFFEADRDNETPVVYGRSWKASELRLKSWDDLHKLWYVLLKEKNMLMTQRQMLQAQNLRFPNPERLPKVRKSMCRIKHVLTERAIEEPDARRSAEMKKMINGL