Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22188.t1 | XP_013459656.1 | 99.5 | 215 | 1 | 0 | 1 | 215 | 1 | 215 | 1.50E-122 | 448.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22188.t1 | O80852 | 60.3 | 214 | 85 | 0 | 1 | 214 | 1 | 214 | 5.6e-78 | 292.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22188.t1 | A0A072UVM9 | 99.5 | 215 | 1 | 0 | 1 | 215 | 1 | 215 | 1.1e-122 | 448.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene000510 | MS.gene22188 | PPI |
| MS.gene22188 | MS.gene048703 | PPI |
| MS.gene04976 | MS.gene22188 | PPI |
| MS.gene22188 | MS.gene69499 | PPI |
| MS.gene005621 | MS.gene22188 | PPI |
| MS.gene22188 | MS.gene48032 | PPI |
| MS.gene22188 | MS.gene69505 | PPI |
| MS.gene22188 | MS.gene017062 | PPI |
| MS.gene22188 | MS.gene07192 | PPI |
| MS.gene038277 | MS.gene22188 | PPI |
| MS.gene20711 | MS.gene22188 | PPI |
| MS.gene008334 | MS.gene22188 | PPI |
| MS.gene008278 | MS.gene22188 | PPI |
| MS.gene22188 | MS.gene048797 | PPI |
| MS.gene019181 | MS.gene22188 | PPI |
| MS.gene008337 | MS.gene22188 | PPI |
| MS.gene017060 | MS.gene22188 | PPI |
| MS.gene019183 | MS.gene22188 | PPI |
| MS.gene22188 | MS.gene049699 | PPI |
| MS.gene061566 | MS.gene22188 | PPI |
| MS.gene47714 | MS.gene22188 | PPI |
| MS.gene22188 | MS.gene83384 | PPI |
| MS.gene22188 | MS.gene72151 | PPI |
| MS.gene22196 | MS.gene22188 | PPI |
| MS.gene22188 | MS.gene22196 | PPI |
| MS.gene030405 | MS.gene22188 | PPI |
| MS.gene22188 | MS.gene48030 | PPI |
| MS.gene22195 | MS.gene22188 | PPI |
| MS.gene22188 | MS.gene049706 | PPI |
| MS.gene03034 | MS.gene22188 | PPI |
| MS.gene22188 | MS.gene05016 | PPI |
| MS.gene048743 | MS.gene22188 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22188.t1 | MTR_3g450930 | 99.535 | 215 | 1 | 0 | 1 | 215 | 1 | 215 | 1.41e-160 | 442 |
| MS.gene22188.t1 | MTR_3g450790 | 77.209 | 215 | 49 | 0 | 1 | 215 | 1 | 215 | 7.20e-128 | 359 |
| MS.gene22188.t1 | MTR_5g090920 | 70.968 | 217 | 60 | 2 | 1 | 215 | 1 | 216 | 3.84e-111 | 317 |
| MS.gene22188.t1 | MTR_5g090910 | 69.907 | 216 | 64 | 1 | 1 | 215 | 1 | 216 | 5.73e-109 | 311 |
| MS.gene22188.t1 | MTR_3g064700 | 56.338 | 213 | 92 | 1 | 1 | 212 | 1 | 213 | 1.90e-88 | 259 |
| MS.gene22188.t1 | MTR_5g090910 | 69.079 | 152 | 46 | 1 | 65 | 215 | 8 | 159 | 2.62e-73 | 219 |
| MS.gene22188.t1 | MTR_1g088850 | 46.190 | 210 | 110 | 3 | 1 | 208 | 1 | 209 | 9.80e-63 | 194 |
| MS.gene22188.t1 | MTR_1g026140 | 44.495 | 218 | 115 | 3 | 3 | 214 | 5 | 222 | 9.51e-62 | 192 |
| MS.gene22188.t1 | MTR_1g088840 | 43.333 | 210 | 117 | 2 | 2 | 210 | 3 | 211 | 2.66e-58 | 183 |
| MS.gene22188.t1 | MTR_1g088825 | 43.333 | 210 | 117 | 2 | 2 | 210 | 3 | 211 | 2.66e-58 | 183 |
| MS.gene22188.t1 | MTR_1g088845 | 42.857 | 210 | 118 | 2 | 2 | 210 | 3 | 211 | 4.38e-55 | 175 |
| MS.gene22188.t1 | MTR_1g088840 | 45.122 | 164 | 89 | 1 | 47 | 210 | 2 | 164 | 1.42e-47 | 154 |
| MS.gene22188.t1 | MTR_1g088840 | 48.673 | 113 | 57 | 1 | 98 | 210 | 4 | 115 | 4.95e-32 | 113 |
| MS.gene22188.t1 | MTR_8g098420 | 30.994 | 171 | 114 | 3 | 1 | 167 | 1 | 171 | 1.77e-18 | 80.1 |
| MS.gene22188.t1 | MTR_8g098420 | 30.994 | 171 | 114 | 3 | 1 | 167 | 1 | 171 | 3.78e-18 | 80.5 |
| MS.gene22188.t1 | MTR_1g492670 | 53.030 | 66 | 30 | 1 | 145 | 210 | 2 | 66 | 8.81e-17 | 72.0 |
| MS.gene22188.t1 | MTR_4g134370 | 31.928 | 166 | 98 | 5 | 11 | 170 | 25 | 181 | 1.55e-12 | 64.7 |
| MS.gene22188.t1 | MTR_4g134380 | 30.178 | 169 | 103 | 5 | 11 | 173 | 25 | 184 | 5.86e-12 | 63.2 |
| MS.gene22188.t1 | MTR_8g098430 | 27.485 | 171 | 116 | 4 | 3 | 167 | 5 | 173 | 4.24e-11 | 60.8 |
| MS.gene22188.t1 | MTR_4g134380 | 30.323 | 155 | 93 | 5 | 11 | 159 | 25 | 170 | 5.45e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22188.t1 | AT2G30860 | 60.280 | 214 | 85 | 0 | 1 | 214 | 1 | 214 | 3.58e-101 | 292 |
| MS.gene22188.t1 | AT2G30870 | 58.605 | 215 | 89 | 0 | 1 | 215 | 1 | 215 | 1.73e-97 | 283 |
| MS.gene22188.t1 | AT3G03190 | 52.336 | 214 | 101 | 1 | 1 | 213 | 1 | 214 | 1.12e-75 | 227 |
| MS.gene22188.t1 | AT5G17220 | 50.711 | 211 | 103 | 1 | 1 | 210 | 1 | 211 | 4.30e-74 | 223 |
| MS.gene22188.t1 | AT2G30860 | 58.824 | 153 | 63 | 0 | 1 | 153 | 1 | 153 | 3.13e-66 | 201 |
| MS.gene22188.t1 | AT3G62760 | 46.890 | 209 | 108 | 3 | 1 | 207 | 1 | 208 | 1.59e-65 | 202 |
| MS.gene22188.t1 | AT1G02930 | 42.512 | 207 | 116 | 3 | 3 | 208 | 4 | 208 | 1.14e-55 | 176 |
| MS.gene22188.t1 | AT1G02930 | 42.512 | 207 | 116 | 3 | 3 | 208 | 4 | 208 | 1.14e-55 | 176 |
| MS.gene22188.t1 | AT2G02930 | 44.286 | 210 | 112 | 3 | 3 | 208 | 4 | 212 | 7.19e-55 | 174 |
| MS.gene22188.t1 | AT4G02520 | 43.062 | 209 | 114 | 3 | 3 | 207 | 4 | 211 | 9.75e-55 | 174 |
| MS.gene22188.t1 | AT1G02920 | 42.512 | 207 | 117 | 2 | 3 | 208 | 4 | 209 | 2.34e-54 | 173 |
| MS.gene22188.t1 | AT2G47730 | 42.857 | 210 | 117 | 3 | 5 | 212 | 54 | 262 | 8.97e-54 | 173 |
| MS.gene22188.t1 | AT2G47730 | 42.857 | 210 | 117 | 3 | 5 | 212 | 54 | 262 | 8.97e-54 | 173 |
| MS.gene22188.t1 | AT1G49860 | 43.367 | 196 | 108 | 2 | 16 | 208 | 19 | 214 | 9.70e-51 | 165 |
| MS.gene22188.t1 | AT1G02950 | 40.686 | 204 | 118 | 3 | 4 | 205 | 26 | 228 | 2.27e-49 | 161 |
| MS.gene22188.t1 | AT1G02950 | 40.686 | 204 | 118 | 3 | 4 | 205 | 26 | 228 | 2.27e-49 | 161 |
| MS.gene22188.t1 | AT1G02950 | 40.686 | 204 | 118 | 3 | 4 | 205 | 28 | 230 | 2.70e-49 | 161 |
| MS.gene22188.t1 | AT1G02950 | 40.686 | 204 | 118 | 3 | 4 | 205 | 38 | 240 | 4.03e-49 | 161 |
| MS.gene22188.t1 | AT1G02940 | 39.713 | 209 | 123 | 3 | 4 | 210 | 40 | 247 | 3.58e-46 | 153 |
| MS.gene22188.t1 | AT1G02940 | 39.713 | 209 | 123 | 3 | 4 | 210 | 40 | 247 | 3.58e-46 | 153 |
| MS.gene22188.t1 | AT1G02940 | 39.713 | 209 | 123 | 3 | 4 | 210 | 53 | 260 | 4.61e-46 | 154 |
| MS.gene22188.t1 | AT1G02940 | 39.713 | 209 | 123 | 3 | 4 | 210 | 65 | 272 | 5.38e-46 | 154 |
| MS.gene22188.t1 | AT1G02950 | 39.583 | 144 | 85 | 2 | 63 | 205 | 60 | 202 | 7.94e-32 | 115 |
| MS.gene22188.t1 | AT5G41210 | 28.655 | 171 | 118 | 3 | 1 | 167 | 2 | 172 | 2.19e-13 | 67.4 |
| MS.gene22188.t1 | AT5G41240 | 28.070 | 171 | 119 | 2 | 1 | 167 | 1 | 171 | 2.52e-13 | 68.6 |
| MS.gene22188.t1 | AT5G41220 | 26.901 | 171 | 121 | 2 | 1 | 167 | 1 | 171 | 2.97e-11 | 62.4 |
Find 63 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTACATGTTGATGGCTTTA+AGG | 0.187628 | 3.4:+40822959 | MS.gene22188:CDS |
| CGTTTCGGGCAAGCATAGTC+TGG | 0.293826 | 3.4:-40822894 | None:intergenic |
| CAACTTGGTTATGAATCTTT+TGG | 0.307735 | 3.4:+40837020 | MS.gene22188:CDS |
| TAGTCACCATCTTGAATAAC+AGG | 0.313539 | 3.4:-40836555 | None:intergenic |
| ACGATGGTTGTGAAGGTATA+TGG | 0.320484 | 3.4:+40822870 | None:intergenic |
| TTCAATGGGAAAAGAGAATA+TGG | 0.323458 | 3.4:+40837218 | MS.gene22188:CDS |
| GACACACAATCACCCGTTTC+GGG | 0.336118 | 3.4:-40822908 | None:intergenic |
| AGCTGAATACCTTAAGTTGC+AGG | 0.348647 | 3.4:+40822995 | MS.gene22188:CDS |
| GATCTTAGCCATCTTCCATT+TGG | 0.361097 | 3.4:+40837180 | MS.gene22188:CDS |
| GACTGGTGGGTTGAAGTTAT+GGG | 0.372883 | 3.4:-40836995 | None:intergenic |
| AGACACACAATCACCCGTTT+CGG | 0.378935 | 3.4:-40822909 | None:intergenic |
| GATGGTGACTATACTCTCTA+TGG | 0.382544 | 3.4:+40836567 | MS.gene22188:CDS |
| AGACTGGTGGGTTGAAGTTA+TGG | 0.387257 | 3.4:-40836996 | None:intergenic |
| GTACATGTTGATGGCTTTAA+GGG | 0.392713 | 3.4:+40822960 | MS.gene22188:CDS |
| AGACTATGCTTGCCCGAAAC+GGG | 0.405607 | 3.4:+40822896 | MS.gene22188:CDS |
| TCATTATTTGATGAATTCAA+TGG | 0.409469 | 3.4:+40837203 | MS.gene22188:CDS |
| AAAGGTGTTGGATATTTATG+AGG | 0.436190 | 3.4:+40837107 | MS.gene22188:CDS |
| AATCAAGGGACTGATTTGCT+TGG | 0.451580 | 3.4:+40836922 | MS.gene22188:CDS |
| TTTGAAACGGTACATGTTGA+TGG | 0.459046 | 3.4:+40822951 | MS.gene22188:CDS |
| TTCATCAAATAATGACCAAA+TGG | 0.459253 | 3.4:-40837195 | None:intergenic |
| CATTATTTGATGAATTCAAT+GGG | 0.460909 | 3.4:+40837204 | MS.gene22188:CDS |
| TGGAAAGACAATAGAAGAAA+GGG | 0.463681 | 3.4:+40836942 | MS.gene22188:CDS |
| CAGACTATGCTTGCCCGAAA+CGG | 0.465174 | 3.4:+40822895 | MS.gene22188:CDS |
| TTGTTTCATATGCAGCCCTT+TGG | 0.467394 | 3.4:+40836525 | MS.gene22188:intron |
| GGTGGGATGATATTAGCAAT+AGG | 0.491315 | 3.4:+40837268 | MS.gene22188:CDS |
| ATATTAGCAATAGGCCATCT+TGG | 0.491886 | 3.4:+40837277 | MS.gene22188:CDS |
| TTACTCCCTGTTATTCAAGA+TGG | 0.495181 | 3.4:+40836549 | MS.gene22188:CDS |
| TTGGAAAGACAATAGAAGAA+AGG | 0.498497 | 3.4:+40836941 | MS.gene22188:CDS |
| CAATAGGCCATCTTGGAAGA+AGG | 0.501103 | 3.4:+40837284 | MS.gene22188:CDS |
| AATAATGACCAAATGGAAGA+TGG | 0.513184 | 3.4:-40837188 | None:intergenic |
| AAAGGATATTGAGTTTGAAA+CGG | 0.517622 | 3.4:+40822938 | MS.gene22188:CDS |
| GGGAAAAGAGAATATGGTAA+AGG | 0.528479 | 3.4:+40837224 | MS.gene22188:CDS |
| AGAAAAGCTTGGAAAGGTGT+TGG | 0.539764 | 3.4:+40837095 | MS.gene22188:CDS |
| CAACCCACCAGTCTACAACT+TGG | 0.543299 | 3.4:+40837005 | MS.gene22188:CDS |
| TTCATAACCAAGTTGTAGAC+TGG | 0.547963 | 3.4:-40837012 | None:intergenic |
| TCAAATTACGATGGTTGTGA+AGG | 0.550550 | 3.4:+40822863 | None:intergenic |
| GCTATCAAAGACTAAGTACT+TGG | 0.554970 | 3.4:+40837134 | MS.gene22188:CDS |
| CAAGAGAGTGAAGAAAAGCT+TGG | 0.555057 | 3.4:+40837084 | MS.gene22188:CDS |
| TCAAAGACTAAGTACTTGGC+TGG | 0.557175 | 3.4:+40837138 | MS.gene22188:CDS |
| GATTGTGTGTCTGATTGAAA+AGG | 0.560625 | 3.4:+40822920 | MS.gene22188:CDS |
| TGTAGAACCTTCTTCCAAGA+TGG | 0.563715 | 3.4:-40837291 | None:intergenic |
| AATAGAAGAAAGGGGTCTTG+TGG | 0.568965 | 3.4:+40836951 | MS.gene22188:CDS |
| CACAAACCCTCTAATCACAT+AGG | 0.571439 | 3.4:-40837327 | None:intergenic |
| GTTTCGGGCAAGCATAGTCT+GGG | 0.573332 | 3.4:-40822893 | None:intergenic |
| AAAGGGGTCTTGTGGAGCAA+TGG | 0.573472 | 3.4:+40836959 | MS.gene22188:CDS |
| TGGCTTTAAGGGAGAGCACA+AGG | 0.573525 | 3.4:+40822971 | MS.gene22188:CDS |
| GAGTGAAGAAAAGCTTGGAA+AGG | 0.576328 | 3.4:+40837089 | MS.gene22188:CDS |
| AGAGGAAGCATGTGAATGCT+TGG | 0.577585 | 3.4:+40837247 | MS.gene22188:CDS |
| ATAACCAAGTTGTAGACTGG+TGG | 0.589687 | 3.4:-40837009 | None:intergenic |
| ACAAACCCTCTAATCACATA+GGG | 0.591213 | 3.4:-40837326 | None:intergenic |
| TGTTGGATATTTATGAGGAG+AGG | 0.593358 | 3.4:+40837112 | MS.gene22188:CDS |
| AATAACAGGGAGTAATCCAA+AGG | 0.597435 | 3.4:-40836541 | None:intergenic |
| CAGAATCTAGGGCAATCCTA+AGG | 0.599160 | 3.4:+40836878 | MS.gene22188:intron |
| AAGAGAATATGGTAAAGGAG+AGG | 0.603130 | 3.4:+40837229 | MS.gene22188:CDS |
| TAACCAAGTTGTAGACTGGT+GGG | 0.611018 | 3.4:-40837008 | None:intergenic |
| GGAAGCATGTGAATGCTTGG+TGG | 0.612663 | 3.4:+40837250 | MS.gene22188:CDS |
| GAAGCATGTGAATGCTTGGT+GGG | 0.616766 | 3.4:+40837251 | MS.gene22188:CDS |
| CAGATGGAAGTCCCACTAGT+GGG | 0.622928 | 3.4:-40837047 | None:intergenic |
| ATAACAGGGAGTAATCCAAA+GGG | 0.626677 | 3.4:-40836540 | None:intergenic |
| TCAGATGGAAGTCCCACTAG+TGG | 0.646315 | 3.4:-40837048 | None:intergenic |
| GGAAAGACAATAGAAGAAAG+GGG | 0.672825 | 3.4:+40836943 | MS.gene22188:CDS |
| GAAGATGGCTAAGATCAGCA+AGG | 0.700476 | 3.4:-40837173 | None:intergenic |
| AGTCACCATCTTGAATAACA+GGG | 0.712338 | 3.4:-40836554 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 40822873 | 40837335 | 40822873 | ID=MS.gene22188 |
| chr3.4 | mRNA | 40822873 | 40837335 | 40822873 | ID=MS.gene22188.t1;Parent=MS.gene22188 |
| chr3.4 | exon | 40822873 | 40823016 | 40822873 | ID=MS.gene22188.t1.exon1;Parent=MS.gene22188.t1 |
| chr3.4 | CDS | 40822873 | 40823016 | 40822873 | ID=cds.MS.gene22188.t1;Parent=MS.gene22188.t1 |
| chr3.4 | exon | 40836540 | 40836588 | 40836540 | ID=MS.gene22188.t1.exon2;Parent=MS.gene22188.t1 |
| chr3.4 | CDS | 40836540 | 40836588 | 40836540 | ID=cds.MS.gene22188.t1;Parent=MS.gene22188.t1 |
| chr3.4 | exon | 40836881 | 40837335 | 40836881 | ID=MS.gene22188.t1.exon3;Parent=MS.gene22188.t1 |
| chr3.4 | CDS | 40836881 | 40837335 | 40836881 | ID=cds.MS.gene22188.t1;Parent=MS.gene22188.t1 |
| Gene Sequence |
| Protein sequence |
