Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23112.t1 | KCW52694.1 | 99 | 103 | 1 | 0 | 1 | 103 | 4 | 106 | 2.80E-47 | 197.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23112.t1 | P62785 | 99.0 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 6.8e-50 | 197.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23112.t1 | M5VRN0 | 99.0 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 2.1e-47 | 197.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene23112 | 0.823001 | 1.75E-53 | -1.69E-46 |
| MS.gene049350 | MS.gene23112 | 0.822348 | 2.48E-53 | -1.69E-46 |
| MS.gene049807 | MS.gene23112 | 0.803948 | 2.73E-49 | -1.69E-46 |
| MS.gene049821 | MS.gene23112 | 0.841113 | 5.78E-58 | -1.69E-46 |
| MS.gene050020 | MS.gene23112 | 0.802009 | 6.87E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene23112 | 0.81509 | 1.10E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene23112 | 0.815504 | 8.94E-52 | -1.69E-46 |
| MS.gene050838 | MS.gene23112 | 0.808025 | 3.79E-50 | -1.69E-46 |
| MS.gene050901 | MS.gene23112 | 0.994966 | 8.67E-212 | -1.69E-46 |
| MS.gene051247 | MS.gene23112 | 0.81523 | 1.03E-51 | -1.69E-46 |
| MS.gene051328 | MS.gene23112 | 0.800628 | 1.32E-48 | -1.69E-46 |
| MS.gene051343 | MS.gene23112 | 0.801293 | 9.64E-49 | -1.69E-46 |
| MS.gene05157 | MS.gene23112 | 0.845832 | 3.18E-59 | -1.69E-46 |
| MS.gene051664 | MS.gene23112 | 0.813684 | 2.26E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene23112 | 0.84346 | 1.38E-58 | -1.69E-46 |
| MS.gene051720 | MS.gene23112 | 0.849056 | 4.13E-60 | -1.69E-46 |
| MS.gene051744 | MS.gene23112 | 0.820703 | 5.96E-53 | -1.69E-46 |
| MS.gene052133 | MS.gene23112 | 0.822353 | 2.48E-53 | -1.69E-46 |
| MS.gene052516 | MS.gene23112 | 0.800078 | 1.71E-48 | -1.69E-46 |
| MS.gene052609 | MS.gene23112 | 0.800853 | 1.19E-48 | -1.69E-46 |
| MS.gene053122 | MS.gene23112 | 0.802774 | 4.78E-49 | -1.69E-46 |
| MS.gene053143 | MS.gene23112 | 0.802895 | 4.51E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene23112 | 0.833036 | 6.72E-56 | -1.69E-46 |
| MS.gene053174 | MS.gene23112 | 0.824024 | 1.01E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene23112 | 0.813577 | 2.39E-51 | -1.69E-46 |
| MS.gene053563 | MS.gene23112 | 0.823129 | 1.64E-53 | -1.69E-46 |
| MS.gene053581 | MS.gene23112 | 0.85616 | 3.88E-62 | -1.69E-46 |
| MS.gene05364 | MS.gene23112 | 0.811309 | 7.47E-51 | -1.69E-46 |
| MS.gene054142 | MS.gene23112 | 0.800394 | 1.47E-48 | -1.69E-46 |
| MS.gene054584 | MS.gene23112 | 0.819636 | 1.05E-52 | -1.69E-46 |
| MS.gene055465 | MS.gene23112 | 0.832662 | 8.32E-56 | -1.69E-46 |
| MS.gene055466 | MS.gene23112 | 0.827203 | 1.78E-54 | -1.69E-46 |
| MS.gene055468 | MS.gene23112 | 0.820914 | 5.33E-53 | -1.69E-46 |
| MS.gene055469 | MS.gene23112 | 0.808498 | 3.01E-50 | -1.69E-46 |
| MS.gene055513 | MS.gene23112 | 0.834395 | 3.07E-56 | -1.69E-46 |
| MS.gene055537 | MS.gene23112 | 0.828634 | 8.06E-55 | -1.69E-46 |
| MS.gene055580 | MS.gene23112 | -0.82585 | 3.74E-54 | -1.69E-46 |
| MS.gene055890 | MS.gene23112 | 0.804179 | 2.45E-49 | -1.69E-46 |
| MS.gene055891 | MS.gene23112 | 0.800644 | 1.31E-48 | -1.69E-46 |
| MS.gene056005 | MS.gene23112 | 0.82324 | 1.54E-53 | -1.69E-46 |
| MS.gene056043 | MS.gene23112 | -0.803401 | 3.55E-49 | -1.69E-46 |
| MS.gene05608 | MS.gene23112 | 0.824419 | 8.16E-54 | -1.69E-46 |
| MS.gene056215 | MS.gene23112 | 0.826416 | 2.75E-54 | -1.69E-46 |
| MS.gene056308 | MS.gene23112 | 0.850952 | 1.22E-60 | -1.69E-46 |
| MS.gene057444 | MS.gene23112 | 0.810573 | 1.08E-50 | -1.69E-46 |
| MS.gene05847 | MS.gene23112 | 0.809324 | 2.00E-50 | -1.69E-46 |
| MS.gene058852 | MS.gene23112 | 0.80934 | 1.99E-50 | -1.69E-46 |
| MS.gene05894 | MS.gene23112 | 0.844706 | 6.40E-59 | -1.69E-46 |
| MS.gene05978 | MS.gene23112 | 0.802413 | 5.68E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene23112 | 0.807646 | 4.57E-50 | -1.69E-46 |
| MS.gene060519 | MS.gene23112 | 0.841095 | 5.85E-58 | -1.69E-46 |
| MS.gene060587 | MS.gene23112 | 0.819423 | 1.17E-52 | -1.69E-46 |
| MS.gene061100 | MS.gene23112 | 0.82227 | 2.59E-53 | -1.69E-46 |
| MS.gene061176 | MS.gene23112 | 0.820741 | 5.84E-53 | -1.69E-46 |
| MS.gene061177 | MS.gene23112 | 0.821105 | 4.82E-53 | -1.69E-46 |
| MS.gene061178 | MS.gene23112 | 0.820895 | 5.39E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23112.t1 | MTR_7g029510 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.33e-68 | 198 |
| MS.gene23112.t1 | MTR_7g099610 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.33e-68 | 198 |
| MS.gene23112.t1 | MTR_5g063620 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.33e-68 | 198 |
| MS.gene23112.t1 | MTR_4g061827 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.33e-68 | 198 |
| MS.gene23112.t1 | MTR_4g061920 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.33e-68 | 198 |
| MS.gene23112.t1 | MTR_3g462400 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.33e-68 | 198 |
| MS.gene23112.t1 | MTR_3g054380 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.33e-68 | 198 |
| MS.gene23112.t1 | MTR_3g462730 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.33e-68 | 198 |
| MS.gene23112.t1 | MTR_3g462510 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.33e-68 | 198 |
| MS.gene23112.t1 | MTR_3g070920 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.33e-68 | 198 |
| MS.gene23112.t1 | MTR_3g061440 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.33e-68 | 198 |
| MS.gene23112.t1 | MTR_3g462410 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.33e-68 | 198 |
| MS.gene23112.t1 | MTR_2g096100 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.33e-68 | 198 |
| MS.gene23112.t1 | MTR_8g038460 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.33e-68 | 198 |
| MS.gene23112.t1 | MTR_4g128150 | 100.000 | 101 | 0 | 0 | 1 | 101 | 1 | 101 | 5.99e-66 | 203 |
| MS.gene23112.t1 | MTR_7g029520 | 84.000 | 50 | 8 | 0 | 27 | 76 | 1 | 50 | 2.85e-21 | 86.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23112.t1 | AT5G59970 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.77e-68 | 198 |
| MS.gene23112.t1 | AT5G59690 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.77e-68 | 198 |
| MS.gene23112.t1 | AT5G59970 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.77e-68 | 198 |
| MS.gene23112.t1 | AT3G46320 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.77e-68 | 198 |
| MS.gene23112.t1 | AT3G45930 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.77e-68 | 198 |
| MS.gene23112.t1 | AT3G53730 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.77e-68 | 198 |
| MS.gene23112.t1 | AT2G28740 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.77e-68 | 198 |
| MS.gene23112.t1 | AT1G07660 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.77e-68 | 198 |
| MS.gene23112.t1 | AT1G07820 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.77e-68 | 198 |
| MS.gene23112.t1 | AT1G07820 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 9.77e-68 | 198 |
| MS.gene23112.t1 | AT1G07660 | 82.524 | 103 | 1 | 1 | 1 | 103 | 1 | 86 | 1.44e-50 | 154 |
Find 32 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGCAGGGAAGGACTTTATA+TGG | 0.314150 | 7.2:+4037723 | MS.gene23112:CDS |
| GGAAAAGGAGGAAAGGGATT+AGG | 0.403262 | 7.2:+4037459 | MS.gene23112:CDS |
| AAAATGTCAGGCAGAGGAAA+AGG | 0.476544 | 7.2:+4037444 | None:intergenic |
| GTGCTTCGTGACAACATTCA+AGG | 0.477240 | 7.2:+4037510 | MS.gene23112:CDS |
| GGCAGAGGAAAAGGAGGAAA+GGG | 0.498699 | 7.2:+4037453 | MS.gene23112:CDS |
| TCACTGCAAGCGAAAATGTC+AGG | 0.502586 | 7.2:+4037432 | None:intergenic |
| CTGCGATTCGTCGTCTTGCA+AGG | 0.502831 | 7.2:+4037544 | MS.gene23112:CDS |
| TGTTTATGCTCTGAAGAGGC+AGG | 0.507468 | 7.2:+4037707 | MS.gene23112:CDS |
| AGGAGGAAAGGGATTAGGAA+AGG | 0.515958 | 7.2:+4037464 | MS.gene23112:CDS |
| GGAGGAAAGGGATTAGGAAA+GGG | 0.527356 | 7.2:+4037465 | MS.gene23112:CDS |
| GGAAAGGGATTAGGAAAGGG+AGG | 0.538569 | 7.2:+4037468 | MS.gene23112:CDS |
| AAGACGACGAATCGCAGGCT+TGG | 0.548379 | 7.2:-4037538 | None:intergenic |
| CTTGCAAGACGACGAATCGC+AGG | 0.552192 | 7.2:-4037543 | None:intergenic |
| AGGCAGAGGAAAAGGAGGAA+AGG | 0.557567 | 7.2:+4037452 | MS.gene23112:CDS |
| ATGCTCTGAAGAGGCAGGGA+AGG | 0.564763 | 7.2:+4037712 | MS.gene23112:CDS |
| AGGACTTTATATGGTTTCAG+TGG | 0.568394 | 7.2:+4037732 | MS.gene23112:CDS |
| GAGGAAGACTGTTACCGCCA+TGG | 0.588179 | 7.2:+4037680 | MS.gene23112:CDS |
| CAAGCGAAAATGTCAGGCAG+AGG | 0.590069 | 7.2:+4037438 | None:intergenic |
| CATTCTCGAGGAAGATCTTG+AGG | 0.597682 | 7.2:-4037620 | None:intergenic |
| TTACATACACTGAACACGCT+AGG | 0.600176 | 7.2:+4037658 | MS.gene23112:CDS |
| GTTTATGCTCTGAAGAGGCA+GGG | 0.605263 | 7.2:+4037708 | MS.gene23112:CDS |
| ATTCGTCGTCTTGCAAGGCG+TGG | 0.611450 | 7.2:+4037549 | MS.gene23112:CDS |
| GGCGGTGTTAAGAGAATCAG+TGG | 0.619776 | 7.2:+4037570 | MS.gene23112:CDS |
| CAGAGCATAAACAACATCCA+TGG | 0.631933 | 7.2:-4037697 | None:intergenic |
| CATACACTGAACACGCTAGG+AGG | 0.635713 | 7.2:+4037661 | MS.gene23112:CDS |
| CGTCGTCTTGCAAGGCGTGG+CGG | 0.636706 | 7.2:+4037552 | MS.gene23112:CDS |
| ATGTCAGGCAGAGGAAAAGG+AGG | 0.661131 | 7.2:+4037447 | MS.gene23112:CDS |
| ATGTTGTTTATGCTCTGAAG+AGG | 0.672733 | 7.2:+4037703 | MS.gene23112:CDS |
| TAGGAAAGGGAGGAGCAAAG+AGG | 0.691969 | 7.2:+4037478 | MS.gene23112:CDS |
| CTCATATATGAAGAAACTCG+CGG | 0.708309 | 7.2:+4037594 | MS.gene23112:CDS |
| CATCACGAATCACATTCTCG+AGG | 0.731876 | 7.2:-4037632 | None:intergenic |
| AGCATAAACAACATCCATGG+CGG | 0.735882 | 7.2:-4037694 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGGACTTTATATGGTTTCAG+TGG | + | chr7.2:4037732-4037751 | MS.gene23112:CDS | 35.0% | |
| CTCATATATGAAGAAACTCG+CGG | + | chr7.2:4037594-4037613 | MS.gene23112:CDS | 35.0% | |
| !! | ATGTTGTTTATGCTCTGAAG+AGG | + | chr7.2:4037703-4037722 | MS.gene23112:CDS | 35.0% |
| AGCATAAACAACATCCATGG+CGG | - | chr7.2:4037697-4037716 | None:intergenic | 40.0% | |
| CAGAGCATAAACAACATCCA+TGG | - | chr7.2:4037700-4037719 | None:intergenic | 40.0% | |
| TTACATACACTGAACACGCT+AGG | + | chr7.2:4037658-4037677 | MS.gene23112:CDS | 40.0% | |
| AGGAGGAAAGGGATTAGGAA+AGG | + | chr7.2:4037464-4037483 | MS.gene23112:CDS | 45.0% | |
| AGGCAGGGAAGGACTTTATA+TGG | + | chr7.2:4037723-4037742 | MS.gene23112:CDS | 45.0% | |
| CATCACGAATCACATTCTCG+AGG | - | chr7.2:4037635-4037654 | None:intergenic | 45.0% | |
| CATTCTCGAGGAAGATCTTG+AGG | - | chr7.2:4037623-4037642 | None:intergenic | 45.0% | |
| GGAAAAGGAGGAAAGGGATT+AGG | + | chr7.2:4037459-4037478 | MS.gene23112:CDS | 45.0% | |
| GGAGGAAAGGGATTAGGAAA+GGG | + | chr7.2:4037465-4037484 | MS.gene23112:CDS | 45.0% | |
| GTGCTTCGTGACAACATTCA+AGG | + | chr7.2:4037510-4037529 | MS.gene23112:CDS | 45.0% | |
| !! | GTTTATGCTCTGAAGAGGCA+GGG | + | chr7.2:4037708-4037727 | MS.gene23112:CDS | 45.0% |
| !! | TGTTTATGCTCTGAAGAGGC+AGG | + | chr7.2:4037707-4037726 | MS.gene23112:CDS | 45.0% |
| AGGCAGAGGAAAAGGAGGAA+AGG | + | chr7.2:4037452-4037471 | MS.gene23112:CDS | 50.0% | |
| ATGTCAGGCAGAGGAAAAGG+AGG | + | chr7.2:4037447-4037466 | MS.gene23112:CDS | 50.0% | |
| CATACACTGAACACGCTAGG+AGG | + | chr7.2:4037661-4037680 | MS.gene23112:CDS | 50.0% | |
| GGAAAGGGATTAGGAAAGGG+AGG | + | chr7.2:4037468-4037487 | MS.gene23112:CDS | 50.0% | |
| GGCAGAGGAAAAGGAGGAAA+GGG | + | chr7.2:4037453-4037472 | MS.gene23112:CDS | 50.0% | |
| TAGGAAAGGGAGGAGCAAAG+AGG | + | chr7.2:4037478-4037497 | MS.gene23112:CDS | 50.0% | |
| ! | GGCGGTGTTAAGAGAATCAG+TGG | + | chr7.2:4037570-4037589 | MS.gene23112:CDS | 50.0% |
| AAGACGACGAATCGCAGGCT+TGG | - | chr7.2:4037541-4037560 | None:intergenic | 55.0% | |
| ATTCGTCGTCTTGCAAGGCG+TGG | + | chr7.2:4037549-4037568 | MS.gene23112:CDS | 55.0% | |
| CTGCGATTCGTCGTCTTGCA+AGG | + | chr7.2:4037544-4037563 | MS.gene23112:CDS | 55.0% | |
| CTTGCAAGACGACGAATCGC+AGG | - | chr7.2:4037546-4037565 | None:intergenic | 55.0% | |
| GAGGAAGACTGTTACCGCCA+TGG | + | chr7.2:4037680-4037699 | MS.gene23112:CDS | 55.0% | |
| !! | ATGCTCTGAAGAGGCAGGGA+AGG | + | chr7.2:4037712-4037731 | MS.gene23112:CDS | 55.0% |
| CGTCGTCTTGCAAGGCGTGG+CGG | + | chr7.2:4037552-4037571 | MS.gene23112:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 4037447 | 4037758 | 4037447 | ID=MS.gene23112 |
| chr7.2 | mRNA | 4037447 | 4037758 | 4037447 | ID=MS.gene23112.t1;Parent=MS.gene23112 |
| chr7.2 | exon | 4037447 | 4037758 | 4037447 | ID=MS.gene23112.t1.exon1;Parent=MS.gene23112.t1 |
| chr7.2 | CDS | 4037447 | 4037758 | 4037447 | ID=cds.MS.gene23112.t1;Parent=MS.gene23112.t1 |
| Gene Sequence |
| Protein sequence |
