AlfalfaGEDB | MS.gene25726

M. sativa cultivar XinJiangDaYe / MS.gene25726

KEGG: -
GO: -
PFAM: PF14547.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25726.t1	AAD03487.1	100	84	0	0	298	381	298	381	6.20E-39	171.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25726.t1	Q00451	87.8	82	9	1	300	380	264	345	3.0e-34	147.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25726.t1	Q40336	100.0	84	0	0	298	381	298	381	4.5e-39	171.8

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene25726	-0.804144	2.49E-49	-1.69E-46
MS.gene049298	MS.gene25726	0.851796	7.03E-61	-1.69E-46
MS.gene050170	MS.gene25726	-0.84122	5.42E-58	-1.69E-46
MS.gene050323	MS.gene25726	-0.81616	6.38E-52	-1.69E-46
MS.gene050326	MS.gene25726	0.804261	2.35E-49	-1.69E-46
MS.gene050338	MS.gene25726	0.813641	2.31E-51	-1.69E-46
MS.gene050496	MS.gene25726	-0.802697	4.96E-49	-1.69E-46
MS.gene050868	MS.gene25726	-0.828299	9.71E-55	-1.69E-46
MS.gene050871	MS.gene25726	-0.831645	1.48E-55	-1.69E-46
MS.gene051838	MS.gene25726	-0.825731	3.99E-54	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25726.t1	MTR_4g101270	69.412	85	26	0	295	379	103	187	2.27e-33	123
MS.gene25726.t1	MTR_1g012700	46.970	132	55	4	249	379	47	164	5.06e-24	97.4
MS.gene25726.t1	MTR_1g012690	51.613	93	43	2	288	379	63	154	2.34e-23	95.5
MS.gene25726.t1	MTR_1g012630	51.163	86	40	2	295	379	126	210	7.00e-22	93.2
MS.gene25726.t1	MTR_1g012710	55.556	81	34	2	300	379	72	151	1.38e-21	90.5
MS.gene25726.t1	MTR_4g115360	62.222	90	34	0	290	379	144	233	9.16e-19	85.1
MS.gene25726.t1	MTR_4g101330	48.810	84	34	4	300	378	89	168	3.83e-18	81.6
MS.gene25726.t1	MTR_3g106740	49.412	85	34	4	300	379	50	130	2.74e-16	75.1
MS.gene25726.t1	MTR_4g101260	45.882	85	44	2	296	379	49	132	8.89e-16	73.9
MS.gene25726.t1	MTR_2g026760	45.161	93	46	4	287	378	38	126	9.76e-16	73.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25726.t1	AT3G22142	45.812	382	136	17	35	380	1134	1480	4.18e-58	204
MS.gene25726.t1	AT4G15160	78.161	87	17	2	295	379	97	183	1.23e-38	137
MS.gene25726.t1	AT2G10940	49.231	260	117	8	125	378	39	289	3.45e-37	136
MS.gene25726.t1	AT2G10940	49.231	260	117	8	125	378	39	289	3.45e-37	136
MS.gene25726.t1	AT1G62500	72.619	84	23	0	296	379	211	294	3.83e-36	134
MS.gene25726.t1	AT4G12490	49.462	93	46	1	288	379	90	182	7.11e-25	100
MS.gene25726.t1	AT4G12470	53.659	82	37	1	299	379	80	161	4.66e-23	94.7
MS.gene25726.t1	AT4G12500	52.439	82	38	1	299	379	96	177	2.20e-22	93.6
MS.gene25726.t1	AT4G12480	53.659	82	37	1	299	379	87	168	2.31e-22	93.2
MS.gene25726.t1	AT1G62510	50.602	83	34	3	300	378	70	149	3.59e-18	81.3

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Find 153 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTGGTGGTTTAGGTACAT+AGG	0.546797	3.3:-69155737	None:intergenic
AACTGGTGGTTTAGGTACAT+AGG	0.546797	3.3:-69155809	None:intergenic
AAGAAGTTTAAGTCTAATAG+TGG	0.443363	3.3:-69156322	None:intergenic
AATGAGAACCTGAAGAGCAA+GGG	0.587112	3.3:-69156364	None:intergenic
ACAAGTGGTGGTGGAGGACA+TGG	0.555606	3.3:-69156120	None:intergenic
ACAATAGGAGGGTGGTGTGT+TGG	0.537158	3.3:-69155394	None:intergenic
ACAATTGGAGGTGTTGGTGT+TGG	0.522248	3.3:-69156054	None:intergenic
ACAATTGGTGGTTTCACAAT+TGG	0.325867	3.3:-69155952	None:intergenic
ACAATTGGTGGTTTCACAAT+TGG	0.349052	3.3:-69155865	None:intergenic
ACAGGCACAACTGGAGGTAG+AGG	0.553941	3.3:-69155991	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTATTGGAGGTTTAACAAT+TGG	-	chr3.3:69155640-69155659	None:intergenic	30.0%
ATTGGAGGTTTAACAATTGG+TGG	-	chr3.3:69155637-69155656	None:intergenic	35.0%
CAAGAGAAGGATGAAAACAT+TGG	-	chr3.3:69155311-69155330	None:intergenic	35.0%
GGACTCATACATATTGGTAT+CGG	+	chr3.3:69156225-69156244	MS.gene25726:CDS	35.0%
TTAGGAGTAGGAGAATACTT+TGG	-	chr3.3:69155442-69155461	None:intergenic	35.0%
AACTGGTGGTTTAGGTACAT+AGG	-	chr3.3:69155740-69155759	None:intergenic	40.0%
AATGAGAACCTGAAGAGCAA+GGG	-	chr3.3:69156367-69156386	None:intergenic	40.0%
ACATGTTGTCCATTGCTTCA+AGG	+	chr3.3:69156264-69156283	MS.gene25726:CDS	40.0%
ACGTAAGGTGGATGTACTAT+TGG	-	chr3.3:69155655-69155674	None:intergenic	40.0%
ACTGGTGGTTTAGGTACATA+GGG	-	chr3.3:69155739-69155758	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	69155289	69156434	69155289	ID=MS.gene25726
chr3.3	mRNA	69155289	69156434	69155289	ID=MS.gene25726.t1;Parent=MS.gene25726
chr3.3	exon	69155289	69156434	69155289	ID=MS.gene25726.t1.exon1;Parent=MS.gene25726.t1
chr3.3	CDS	69155289	69156434	69155289	ID=cds.MS.gene25726.t1;Parent=MS.gene25726.t1

Gene Sequence

>MS.gene25726

ATGGCTAACTATGCTCTAGCCAATGTTTTCATCCTTCTCTTGAACTTGAGTACCTTACTCAATGTTCTTGCTTGCCCTTATTGTCCTTACCCAAGCCCTAAACCACCAACACACCACCCTCCTATTGTTAAGCCACCAGTTCACAAACCACCAAAGTATTCTCCTACTCCTAAACCACCAGTTCACAAACCACCAAGATATCCTCCTAAACCATCACCTTGTCCACCCCCATCATCAACACCAAAACCACCACATGTTCCAAAACCTCCTCATCATCCCAAACCCCCAGTAGTGCACCCACCACATGTTCCAAAACCCCCAGTACATCCACCTTATGTGCCAAAACCACCAATTGTTAAACCTCCAATAGTACATCCACCTTACGTACCAAAACCCCCGGTTGTGAAACCACCACCATATGTCCCTAAGCCACCTGTTGTGAGACCACCCTATGTACCTAAACCACCAGTTGTCCCTGTAACACCACCTTATGTTCCTAAGCCACCTGTTGTGAGACCACCCTATGTACCTAAACCACCAGTTGTCCCTGTAACACCACCTTATGTTCCTAAGCCACCAATTGTGAAACCACCAATTGTTTTCCCACCACATGTTCCACTACCTCCAGTTGTACCATCACCGCCACCTTATGTCCCAACACCTCCAATTGTGAAACCACCAATTGTTTTTCCACCACATGTTCCTCTACCTCCAGTTGTGCCTGTAACACCACCTTATGTGCAACCACCACCTATTGTAACACCACCAACACCAACACCTCCAATTGTGACACCACCAGTGGTTACACCACCAACACCACCTAGTGAAACACCATGTCCTCCACCACCACTTGTACCTTATCCACCTACACCACCAGCACAACAAACATGTTCCATTGATGCTTTGAAGTTGGGTGCATGTGTTGATGTGCTTGGTGGACTCATACATATTGGTATCGGTGGAAGTGCTAAACAAACATGTTGTCCATTGCTTCAAGGACTTGTTGATTTGGATGCAGCTATATGTCTCTGCACCACTATTAGACTTAAACTTCTTAATATCAATCTTGTTATCCCCCTTGCTCTTCAGGTTCTCATTGATTGTGGCAAAACTCCACCAGAAGGGTTTAAGTGCCCAGCTTATTAA

Protein sequence

>MS.gene25726.t1

MANYALANVFILLLNLSTLLNVLACPYCPYPSPKPPTHHPPIVKPPVHKPPKYSPTPKPPVHKPPRYPPKPSPCPPPSSTPKPPHVPKPPHHPKPPVVHPPHVPKPPVHPPYVPKPPIVKPPIVHPPYVPKPPVVKPPPYVPKPPVVRPPYVPKPPVVPVTPPYVPKPPVVRPPYVPKPPVVPVTPPYVPKPPIVKPPIVFPPHVPLPPVVPSPPPYVPTPPIVKPPIVFPPHVPLPPVVPVTPPYVQPPPIVTPPTPTPPIVTPPVVTPPTPPSETPCPPPPLVPYPPTPPAQQTCSIDALKLGACVDVLGGLIHIGIGGSAKQTCCPLLQGLVDLDAAICLCTTIRLKLLNINLVIPLALQVLIDCGKTPPEGFKCPAY