AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32976


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32976.t1 MTR_1g016570 76.630 184 21 1 1 184 204 365 2.02e-96 284
MS.gene32976.t1 MTR_1g016540 68.617 188 53 4 1 185 1 185 3.88e-85 249
MS.gene32976.t1 MTR_3g030080 63.889 180 55 2 1 180 204 373 1.04e-72 224
MS.gene32976.t1 MTR_2g047180 61.667 180 51 5 7 183 206 370 5.85e-65 204
MS.gene32976.t1 MTR_3g029650 58.470 183 64 3 1 180 189 362 1.49e-63 209
MS.gene32976.t1 MTR_3g029650 46.237 186 84 7 1 180 576 751 2.31e-36 134
MS.gene32976.t1 MTR_3g030380 55.556 180 63 3 1 180 196 358 2.68e-55 179
MS.gene32976.t1 MTR_3g092000 54.167 192 68 4 1 186 229 406 5.22e-55 179
MS.gene32976.t1 MTR_1g049170 55.801 181 64 5 1 178 216 383 9.94e-55 178
MS.gene32976.t1 MTR_3g030480 56.354 181 55 5 1 178 194 353 2.86e-53 173
MS.gene32976.t1 MTR_3g030440 55.801 181 56 5 1 178 195 354 3.09e-53 173
MS.gene32976.t1 MTR_6g018315 52.459 183 76 4 1 178 189 365 2.82e-52 172
MS.gene32976.t1 MTR_3g091990 54.545 187 72 5 1 184 215 391 1.81e-51 170
MS.gene32976.t1 MTR_6g018390 52.809 178 68 5 1 178 204 365 7.38e-50 165
MS.gene32976.t1 MTR_6g018360 50.820 183 79 4 1 178 204 380 1.12e-47 160
MS.gene32976.t1 MTR_3g030360 51.099 182 52 4 1 181 208 353 1.23e-46 156
MS.gene32976.t1 MTR_6g027040 52.222 180 68 5 1 178 212 375 2.36e-46 156
MS.gene32976.t1 MTR_6g027100 52.222 180 68 5 1 178 212 375 2.95e-46 157
MS.gene32976.t1 MTR_5g042230 51.786 168 74 3 1 167 27 188 4.97e-46 150
MS.gene32976.t1 MTR_3g029920 49.727 183 76 5 2 181 212 381 4.27e-45 153
MS.gene32976.t1 MTR_3g029750 47.222 180 83 5 1 180 220 387 6.88e-45 152
MS.gene32976.t1 MTR_3g029620 51.381 181 67 8 6 182 213 376 1.74e-44 151
MS.gene32976.t1 MTR_3g037710 44.615 195 78 5 1 178 81 262 7.80e-44 147
MS.gene32976.t1 MTR_1g007250 51.462 171 72 4 1 171 1 160 1.19e-43 143
MS.gene32976.t1 MTR_3g029670 48.333 180 81 5 1 180 220 387 1.25e-43 149
MS.gene32976.t1 MTR_3g030050 42.991 214 77 5 1 178 216 420 4.23e-43 149
MS.gene32976.t1 MTR_1g007260 50.575 174 75 5 1 174 221 383 7.49e-43 147
MS.gene32976.t1 MTR_3g029640 49.718 177 68 7 6 178 204 363 2.79e-42 145
MS.gene32976.t1 MTR_3g030355 45.312 192 81 5 1 180 216 395 4.15e-42 145
MS.gene32976.t1 MTR_1g026850 49.171 181 78 5 1 178 194 363 1.21e-41 144
MS.gene32976.t1 MTR_6g018400 47.253 182 73 4 1 178 206 368 3.11e-41 142
MS.gene32976.t1 MTR_5g078450 52.113 142 46 1 44 185 5 124 2.45e-40 133
MS.gene32976.t1 MTR_3g029900 50.000 180 63 6 1 178 303 457 4.20e-39 139
MS.gene32976.t1 MTR_3g030560 48.023 177 74 5 6 178 210 372 1.93e-38 135
MS.gene32976.t1 MTR_3g029840 48.108 185 60 7 1 180 203 356 1.13e-36 130
MS.gene32976.t1 MTR_3g029820 50.000 180 66 8 7 180 167 328 1.16e-36 130
MS.gene32976.t1 MTR_3g029800 45.604 182 74 7 1 178 195 355 4.46e-36 129
MS.gene32976.t1 MTR_1g007220 45.349 172 55 3 1 171 34 167 5.74e-36 124
MS.gene32976.t1 MTR_3g047980 45.856 181 53 7 6 178 216 359 8.45e-34 123
MS.gene32976.t1 MTR_3g029950 44.792 192 68 8 1 180 203 368 1.57e-33 122
MS.gene32976.t1 MTR_3g058460 42.781 187 88 5 1 178 199 375 1.54e-32 120
MS.gene32976.t1 MTR_6g018370 52.500 120 48 3 1 117 146 259 2.89e-29 108
MS.gene32976.t1 MTR_3g029870 42.222 180 65 6 14 180 182 335 5.40e-29 110
MS.gene32976.t1 MTR_4g022880 43.478 138 70 2 50 181 39 174 4.28e-28 103
MS.gene32976.t1 MTR_7g005990 39.444 180 67 6 9 180 213 358 1.92e-24 98.2
MS.gene32976.t1 MTR_1g007200 49.038 104 40 3 62 163 1 93 1.49e-20 82.8
MS.gene32976.t1 MTR_8g035995 46.535 101 47 2 10 110 1 94 9.58e-18 76.3
MS.gene32976.t1 MTR_8g074610 30.108 186 102 5 4 178 227 395 1.04e-15 74.3
MS.gene32976.t1 MTR_2g041510 36.620 142 73 4 4 133 200 336 2.96e-14 70.1
MS.gene32976.t1 MTR_2g041510 36.620 142 73 4 4 133 220 356 3.11e-14 70.1
MS.gene32976.t1 MTR_8g074640 33.929 168 82 6 2 154 206 359 2.74e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32976.t1 AT2G17030 36.842 190 96 5 7 178 207 390 2.44e-26 103
MS.gene32976.t1 AT2G17036 34.409 186 101 5 7 178 205 383 6.90e-25 100
MS.gene32976.t1 AT2G17036 34.409 186 101 5 7 178 206 384 6.97e-25 100

Find 39 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATGAGAAGGAGAGTAAA+TGG 0.206024 1.1:+5670902 MS.gene32976:CDS
ATGGGTTGATTTGATGAGTT+TGG 0.251492 1.1:+5670921 MS.gene32976:CDS
TTAACCACAAGTTCATATTT+AGG 0.261634 1.1:-5670760 None:intergenic
ATTTGATGAGTTTGGGAGAT+AGG 0.289011 1.1:+5670929 MS.gene32976:CDS
AAATATGAACTTGTGGTTAA+TGG 0.323243 1.1:+5670763 MS.gene32976:CDS
GTGGACATTTATGAGTGTTT+AGG 0.334870 1.1:+5670832 MS.gene32976:CDS
CCAATTCCATCAATCATATA+AGG 0.345857 1.1:-5670682 None:intergenic
CGGACAATTATTGTTGGACT+TGG 0.356140 1.1:+5670706 MS.gene32976:CDS
TTCACCTTTGTCTGATTGTC+CGG 0.356602 1.1:+5671113 MS.gene32976:CDS
GCAAGATAACATACTCTGTT+AGG 0.372490 1.1:-5671189 None:intergenic
CAGAACAAGTTAAAATATTC+CGG 0.384086 1.1:-5671132 None:intergenic
CCTTATATGATTGATGGAAT+TGG 0.407615 1.1:+5670682 MS.gene32976:CDS
TACTGATATGTCTATGTACT+TGG 0.428125 1.1:+5670633 None:intergenic
TGATGAGAAGGAGAGTAAAT+GGG 0.446912 1.1:+5670903 MS.gene32976:CDS
TGTATCTTTGACTTGGATCA+AGG 0.447583 1.1:+5671084 MS.gene32976:CDS
GGGCGGCCTTATATGATTGA+TGG 0.452306 1.1:+5670676 MS.gene32976:CDS
TGGGTTGATTTGATGAGTTT+GGG 0.459847 1.1:+5670922 MS.gene32976:CDS
TGTGTTTAGGTTTGATGAGA+AGG 0.463451 1.1:+5670891 MS.gene32976:CDS
TTTGATGAGTTTGGGAGATA+GGG 0.474756 1.1:+5670930 MS.gene32976:CDS
GATCGCATGACTTCGATGAC+TGG 0.480649 1.1:+5670728 MS.gene32976:CDS
ATTGGTCGGACAATTATTGT+TGG 0.482444 1.1:+5670700 MS.gene32976:CDS
CAGATTTGTGTGTTTCTAGA+GGG 0.519276 1.1:+5670995 MS.gene32976:CDS
TGGGAACTGTATCTTTGACT+TGG 0.526556 1.1:+5671077 MS.gene32976:CDS
TATGAACTTGTGGTTAATGG+TGG 0.543129 1.1:+5670766 MS.gene32976:CDS
TGAGGGCGCGTTGCTGCGAG+TGG 0.554191 1.1:+5670813 MS.gene32976:CDS
TCAGATTTGTGTGTTTCTAG+AGG 0.559601 1.1:+5670994 MS.gene32976:CDS
TGAACTTGTGGTTAATGGTG+GGG 0.569319 1.1:+5670768 MS.gene32976:CDS
GAACTTGTGGTTAATGGTGG+GGG 0.582058 1.1:+5670769 MS.gene32976:CDS
ATGAACTTGTGGTTAATGGT+GGG 0.584883 1.1:+5670767 MS.gene32976:CDS
TTGTCGATCCACTCTGTAGG+TGG 0.596783 1.1:-5671156 None:intergenic
ACCACCTAAATATGAACTTG+TGG 0.597935 1.1:+5670756 MS.gene32976:CDS
ACTGATATGTCTATGTACTT+GGG 0.602971 1.1:+5670634 None:intergenic
ACCACAAGTTCATATTTAGG+TGG 0.605610 1.1:-5670757 None:intergenic
AAAATTCTTAGTGGAGAGTG+AGG 0.612751 1.1:+5670795 MS.gene32976:CDS
CATTTGTCGATCCACTCTGT+AGG 0.617177 1.1:-5671159 None:intergenic
ATATGATTGATGGAATTGGT+CGG 0.636318 1.1:+5670686 MS.gene32976:CDS
AAATTCTTAGTGGAGAGTGA+GGG 0.668174 1.1:+5670796 MS.gene32976:CDS
TATTCCGGACAATCAGACAA+AGG 0.675553 1.1:-5671117 None:intergenic
TGTTCTGGCCACCTACAGAG+TGG 0.683477 1.1:+5671148 MS.gene32976:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGTAAAAACATGCAATA+TGG + chr1.1:5671057-5671076 MS.gene32976:CDS 20.0%
!! ATTGTAAAAACATGCAATAT+GGG + chr1.1:5671058-5671077 MS.gene32976:CDS 20.0%
!!! TTTTAAGGATTAATGTGTTT+AGG + chr1.1:5670878-5670897 MS.gene32976:CDS 20.0%
! AAATATGAACTTGTGGTTAA+TGG + chr1.1:5670763-5670782 MS.gene32976:CDS 25.0%
! CAGAACAAGTTAAAATATTC+CGG - chr1.1:5671135-5671154 None:intergenic 25.0%
! GTTGAAAAGATTATCTAATC+TGG - chr1.1:5671238-5671257 None:intergenic 25.0%
! TTAACCACAAGTTCATATTT+AGG - chr1.1:5670763-5670782 None:intergenic 25.0%
!!! TGTTTAGGTTTTGATTACTT+CGG + chr1.1:5670847-5670866 MS.gene32976:CDS 25.0%
AAGATTATCTAATCTGGCAA+CGG - chr1.1:5671232-5671251 None:intergenic 30.0%
ATATGATTGATGGAATTGGT+CGG + chr1.1:5670686-5670705 MS.gene32976:CDS 30.0%
CCAATTCCATCAATCATATA+AGG - chr1.1:5670685-5670704 None:intergenic 30.0%
CCTTATATGATTGATGGAAT+TGG + chr1.1:5670682-5670701 MS.gene32976:CDS 30.0%
! ACTTCGGTAAGAATGTTTTA+AGG + chr1.1:5670863-5670882 MS.gene32976:CDS 30.0%
!!! CGGAATATTTTAACTTGTTC+TGG + chr1.1:5671133-5671152 MS.gene32976:CDS 30.0%
AAAATTCTTAGTGGAGAGTG+AGG + chr1.1:5670795-5670814 MS.gene32976:CDS 35.0%
AAATTCTTAGTGGAGAGTGA+GGG + chr1.1:5670796-5670815 MS.gene32976:CDS 35.0%
ACCACAAGTTCATATTTAGG+TGG - chr1.1:5670760-5670779 None:intergenic 35.0%
ACCACCTAAATATGAACTTG+TGG + chr1.1:5670756-5670775 MS.gene32976:CDS 35.0%
ATGAACTTGTGGTTAATGGT+GGG + chr1.1:5670767-5670786 MS.gene32976:CDS 35.0%
ATTGGTCGGACAATTATTGT+TGG + chr1.1:5670700-5670719 MS.gene32976:CDS 35.0%
ATTTGATGAGTTTGGGAGAT+AGG + chr1.1:5670929-5670948 MS.gene32976:CDS 35.0%
GCAAGATAACATACTCTGTT+AGG - chr1.1:5671192-5671211 None:intergenic 35.0%
GTGGACATTTATGAGTGTTT+AGG + chr1.1:5670832-5670851 MS.gene32976:CDS 35.0%
TATGAACTTGTGGTTAATGG+TGG + chr1.1:5670766-5670785 MS.gene32976:CDS 35.0%
TGATGAGAAGGAGAGTAAAT+GGG + chr1.1:5670903-5670922 MS.gene32976:CDS 35.0%
TTGATGAGAAGGAGAGTAAA+TGG + chr1.1:5670902-5670921 MS.gene32976:CDS 35.0%
TTTGATGAGTTTGGGAGATA+GGG + chr1.1:5670930-5670949 MS.gene32976:CDS 35.0%
! TGTGTTTAGGTTTGATGAGA+AGG + chr1.1:5670891-5670910 MS.gene32976:CDS 35.0%
!! ATGGGTTGATTTGATGAGTT+TGG + chr1.1:5670921-5670940 MS.gene32976:CDS 35.0%
!! CAGATTTGTGTGTTTCTAGA+GGG + chr1.1:5670995-5671014 MS.gene32976:CDS 35.0%
!! TCAGATTTGTGTGTTTCTAG+AGG + chr1.1:5670994-5671013 MS.gene32976:CDS 35.0%
!! TGGGTTGATTTGATGAGTTT+GGG + chr1.1:5670922-5670941 MS.gene32976:CDS 35.0%
!! TGTATCTTTGACTTGGATCA+AGG + chr1.1:5671084-5671103 MS.gene32976:CDS 35.0%
!!! GAGATAGGGTTTTGTTTTTG+GGG + chr1.1:5670944-5670963 MS.gene32976:CDS 35.0%
!!! GGAGATAGGGTTTTGTTTTT+GGG + chr1.1:5670943-5670962 MS.gene32976:CDS 35.0%
CGGACAATTATTGTTGGACT+TGG + chr1.1:5670706-5670725 MS.gene32976:CDS 40.0%
TATTCCGGACAATCAGACAA+AGG - chr1.1:5671120-5671139 None:intergenic 40.0%
TGAACTTGTGGTTAATGGTG+GGG + chr1.1:5670768-5670787 MS.gene32976:CDS 40.0%
TGGGAACTGTATCTTTGACT+TGG + chr1.1:5671077-5671096 MS.gene32976:CDS 40.0%
TGGGGGCAAAAAATTCTTAG+TGG + chr1.1:5670786-5670805 MS.gene32976:CDS 40.0%
TTCACCTTTGTCTGATTGTC+CGG + chr1.1:5671113-5671132 MS.gene32976:CDS 40.0%
! GAGACATCTGCGTTTTCAAA+GGG + chr1.1:5670656-5670675 MS.gene32976:CDS 40.0%
!!! AGGGTTTTGTTTTTGGGGAA+TGG + chr1.1:5670949-5670968 MS.gene32976:CDS 40.0%
!!! GGGAGATAGGGTTTTGTTTT+TGG + chr1.1:5670942-5670961 MS.gene32976:CDS 40.0%
CATTTGTCGATCCACTCTGT+AGG - chr1.1:5671162-5671181 None:intergenic 45.0%
GAACTTGTGGTTAATGGTGG+GGG + chr1.1:5670769-5670788 MS.gene32976:CDS 45.0%
! ACATCTGCGTTTTCAAAGGG+CGG + chr1.1:5670659-5670678 MS.gene32976:CDS 45.0%
! GGAGACATCTGCGTTTTCAA+AGG + chr1.1:5670655-5670674 MS.gene32976:CDS 45.0%
GATCGCATGACTTCGATGAC+TGG + chr1.1:5670728-5670747 MS.gene32976:CDS 50.0%
TTGTCGATCCACTCTGTAGG+TGG - chr1.1:5671159-5671178 None:intergenic 50.0%
! GGGCGGCCTTATATGATTGA+TGG + chr1.1:5670676-5670695 MS.gene32976:CDS 50.0%
TGTTCTGGCCACCTACAGAG+TGG + chr1.1:5671148-5671167 MS.gene32976:CDS 55.0%
! TGAGGGCGCGTTGCTGCGAG+TGG + chr1.1:5670813-5670832 MS.gene32976:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 5670640 5671274 5670640 ID=MS.gene32976
chr1.1 mRNA 5670640 5671274 5670640 ID=MS.gene32976.t1;Parent=MS.gene32976
chr1.1 exon 5670640 5671197 5670640 ID=MS.gene32976.t1.exon1;Parent=MS.gene32976.t1
chr1.1 CDS 5670640 5671197 5670640 ID=cds.MS.gene32976.t1;Parent=MS.gene32976.t1
chr1.1 exon 5671269 5671274 5671269 ID=MS.gene32976.t1.exon2;Parent=MS.gene32976.t1
chr1.1 CDS 5671269 5671274 5671269 ID=cds.MS.gene32976.t1;Parent=MS.gene32976.t1
Gene Sequence

>MS.gene32976

ATGTCTATGTACTTGGGAGACATCTGCGTTTTCAAAGGGCGGCCTTATATGATTGATGGAATTGGTCGGACAATTATTGTTGGACTTGGATCGCATGACTTCGATGACTGGTTTGAACCACCTAAATATGAACTTGTGGTTAATGGTGGGGGCAAAAAATTCTTAGTGGAGAGTGAGGGCGCGTTGCTGCGAGTGGACATTTATGAGTGTTTAGGTTTTGATTACTTCGGTAAGAATGTTTTAAGGATTAATGTGTTTAGGTTTGATGAGAAGGAGAGTAAATGGGTTGATTTGATGAGTTTGGGAGATAGGGTTTTGTTTTTGGGGAATGGATGTTCGTTTTCTGCTTCTGCTTCAGATTTGTGTGTTTCTAGAGGGAATTGTGTCATCTTTATTGATGTTGCATACTTCAATTTTAATTGTAAAAACATGCAATATGGGAACTGTATCTTTGACTTGGATCAAGGCAAGCTTTCACCTTTGTCTGATTGTCCGGAATATTTTAACTTGTTCTGGCCACCTACAGAGTGGATCGACAAATGCTGCATTCCTAACAGAGTATGTTATCTTGCATTATTTTGCTTAGTTTCCGTTGCCAGATTAGATAATCTTTTCAACTGTACTTGCAGAAATAG

Protein sequence

>MS.gene32976.t1

MSMYLGDICVFKGRPYMIDGIGRTIIVGLGSHDFDDWFEPPKYELVVNGGGKKFLVESEGALLRVDIYECLGFDYFGKNVLRINVFRFDEKESKWVDLMSLGDRVLFLGNGCSFSASASDLCVSRGNCVIFIDVAYFNFNCKNMQYGNCIFDLDQGKLSPLSDCPEYFNLFWPPTEWIDKCCIPNRK