Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34925.t1 | RHN42391.1 | 97.1 | 139 | 4 | 0 | 2 | 140 | 49 | 187 | 6.80E-68 | 266.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34925.t1 | Q56XJ7 | 33.0 | 103 | 68 | 1 | 11 | 113 | 117 | 218 | 2.6e-07 | 56.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34925.t1 | A0A396GQD5 | 97.1 | 139 | 4 | 0 | 2 | 140 | 49 | 187 | 4.9e-68 | 266.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene34925 | 0.812883 | 3.39E-51 | -1.69E-46 |
| MS.gene049368 | MS.gene34925 | 0.80158 | 8.42E-49 | -1.69E-46 |
| MS.gene049411 | MS.gene34925 | 0.817912 | 2.58E-52 | -1.69E-46 |
| MS.gene049983 | MS.gene34925 | 0.816436 | 5.54E-52 | -1.69E-46 |
| MS.gene050085 | MS.gene34925 | 0.820171 | 7.90E-53 | -1.69E-46 |
| MS.gene050646 | MS.gene34925 | 0.80553 | 1.28E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene34925 | 0.855692 | 5.32E-62 | -1.69E-46 |
| MS.gene050689 | MS.gene34925 | 0.833412 | 5.41E-56 | -1.69E-46 |
| MS.gene050752 | MS.gene34925 | 0.825993 | 3.46E-54 | -1.69E-46 |
| MS.gene050838 | MS.gene34925 | 0.855395 | 6.49E-62 | -1.69E-46 |
| MS.gene050941 | MS.gene34925 | 0.81423 | 1.71E-51 | -1.69E-46 |
| MS.gene050955 | MS.gene34925 | 0.803037 | 4.22E-49 | -1.69E-46 |
| MS.gene051003 | MS.gene34925 | 0.91328 | 7.38E-84 | -1.69E-46 |
| MS.gene051234 | MS.gene34925 | 0.875948 | 2.06E-68 | -1.69E-46 |
| MS.gene051343 | MS.gene34925 | 0.826832 | 2.19E-54 | -1.69E-46 |
| MS.gene051381 | MS.gene34925 | 0.809755 | 1.62E-50 | -1.69E-46 |
| MS.gene05188 | MS.gene34925 | 0.83254 | 8.92E-56 | -1.69E-46 |
| MS.gene051975 | MS.gene34925 | 0.841171 | 5.59E-58 | -1.69E-46 |
| MS.gene052072 | MS.gene34925 | 0.826301 | 2.92E-54 | -1.69E-46 |
| MS.gene052307 | MS.gene34925 | 0.815915 | 7.24E-52 | -1.69E-46 |
| MS.gene052335 | MS.gene34925 | 0.815522 | 8.86E-52 | -1.69E-46 |
| MS.gene052342 | MS.gene34925 | 0.855019 | 8.35E-62 | -1.69E-46 |
| MS.gene052434 | MS.gene34925 | 0.853359 | 2.52E-61 | -1.69E-46 |
| MS.gene053058 | MS.gene34925 | 0.874263 | 7.74E-68 | -1.69E-46 |
| MS.gene054004 | MS.gene34925 | 0.843866 | 1.08E-58 | -1.69E-46 |
| MS.gene054005 | MS.gene34925 | 0.81174 | 6.02E-51 | -1.69E-46 |
| MS.gene054403 | MS.gene34925 | 0.825265 | 5.15E-54 | -1.69E-46 |
| MS.gene05517 | MS.gene34925 | 0.801258 | 9.80E-49 | -1.69E-46 |
| MS.gene055311 | MS.gene34925 | 0.806983 | 6.31E-50 | -1.69E-46 |
| MS.gene055513 | MS.gene34925 | 0.835885 | 1.29E-56 | -1.69E-46 |
| MS.gene055864 | MS.gene34925 | 0.822742 | 2.01E-53 | -1.69E-46 |
| MS.gene056097 | MS.gene34925 | 0.821435 | 4.04E-53 | -1.69E-46 |
| MS.gene056181 | MS.gene34925 | 0.854747 | 1.00E-61 | -1.69E-46 |
| MS.gene056699 | MS.gene34925 | 0.811364 | 7.27E-51 | -1.69E-46 |
| MS.gene056787 | MS.gene34925 | 0.829354 | 5.39E-55 | -1.69E-46 |
| MS.gene056986 | MS.gene34925 | 0.835937 | 1.25E-56 | -1.69E-46 |
| MS.gene057102 | MS.gene34925 | 0.829783 | 4.24E-55 | -1.69E-46 |
| MS.gene05781 | MS.gene34925 | 0.8091 | 2.24E-50 | -1.69E-46 |
| MS.gene058095 | MS.gene34925 | 0.816728 | 4.76E-52 | -1.69E-46 |
| MS.gene058149 | MS.gene34925 | 0.806599 | 7.61E-50 | -1.69E-46 |
| MS.gene058758 | MS.gene34925 | 0.804853 | 1.77E-49 | -1.69E-46 |
| MS.gene059146 | MS.gene34925 | 0.886243 | 4.05E-72 | -1.69E-46 |
| MS.gene059170 | MS.gene34925 | 0.805376 | 1.38E-49 | -1.69E-46 |
| MS.gene059360 | MS.gene34925 | 0.801861 | 7.37E-49 | -1.69E-46 |
| MS.gene059901 | MS.gene34925 | -0.81948 | 1.14E-52 | -1.69E-46 |
| MS.gene06010 | MS.gene34925 | 0.82462 | 7.31E-54 | -1.69E-46 |
| MS.gene06011 | MS.gene34925 | 0.857976 | 1.13E-62 | -1.69E-46 |
| MS.gene060763 | MS.gene34925 | 0.839738 | 1.32E-57 | -1.69E-46 |
| MS.gene060935 | MS.gene34925 | 0.806787 | 6.94E-50 | -1.69E-46 |
| MS.gene060937 | MS.gene34925 | 0.817292 | 3.56E-52 | -1.69E-46 |
| MS.gene061100 | MS.gene34925 | 0.841747 | 3.94E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34925.t1 | MTR_1g031680 | 67.883 | 137 | 38 | 5 | 1 | 132 | 1 | 136 | 2.55e-56 | 172 |
| MS.gene34925.t1 | MTR_3g065950 | 45.926 | 135 | 68 | 3 | 1 | 133 | 4 | 135 | 7.59e-36 | 120 |
| MS.gene34925.t1 | MTR_3g065960 | 46.154 | 130 | 67 | 2 | 4 | 133 | 9 | 135 | 3.50e-33 | 114 |
| MS.gene34925.t1 | MTR_5g078750 | 50.413 | 121 | 54 | 4 | 17 | 135 | 41 | 157 | 2.28e-31 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34925.t1 | AT1G70780 | 59.286 | 140 | 43 | 5 | 1 | 133 | 2 | 134 | 1.13e-46 | 148 |
| MS.gene34925.t1 | AT1G23150 | 57.664 | 137 | 50 | 5 | 1 | 132 | 1 | 134 | 4.43e-39 | 129 |
| MS.gene34925.t1 | AT5G37730 | 50.588 | 85 | 41 | 1 | 16 | 100 | 16 | 99 | 5.39e-25 | 94.4 |
Find 37 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGATCAACATCAATGTTT+TGG | 0.229459 | 4.1:+16235947 | MS.gene34925:CDS |
| CGCTGGACCGATTCGATTTG+TGG | 0.324818 | 4.1:+16235974 | MS.gene34925:CDS |
| GCCATTCCGTTGGTTGTCTC+TGG | 0.349243 | 4.1:-16236708 | None:intergenic |
| GGAATGGGAGCTTGAAGAAT+TGG | 0.358621 | 4.1:+16236766 | MS.gene34925:CDS |
| CATTGGCTCGCCGCAGGAAT+GGG | 0.380492 | 4.1:+16236751 | MS.gene34925:CDS |
| GAGGGAAGACTTCCTATCTT+AGG | 0.398437 | 4.1:+16236056 | MS.gene34925:CDS |
| TTCTCGCATCCTTAGCGCTC+TGG | 0.400064 | 4.1:-16235920 | None:intergenic |
| GCATTGGCTCGCCGCAGGAA+TGG | 0.419805 | 4.1:+16236750 | MS.gene34925:CDS |
| ATCCGCATTGGCCATTCCGT+TGG | 0.457689 | 4.1:-16236718 | None:intergenic |
| GTAACATCCTTTCCTAAGAT+AGG | 0.464705 | 4.1:-16236068 | None:intergenic |
| CGGTCGCATTGGCTCGCCGC+AGG | 0.490804 | 4.1:+16236745 | MS.gene34925:CDS |
| CTGAGTCCACTGGACACCAT+TGG | 0.504639 | 4.1:+16236645 | MS.gene34925:CDS |
| AGCTCCTCCGTGTGATCCAA+TGG | 0.509536 | 4.1:-16236661 | None:intergenic |
| TGCGGATGGAGCGGTCGCAT+TGG | 0.509786 | 4.1:+16236734 | MS.gene34925:CDS |
| TGTCAACACGTTGGATCTGA+TGG | 0.540465 | 4.1:+16236107 | MS.gene34925:CDS |
| TGCGACCGCTCCATCCGCAT+TGG | 0.541505 | 4.1:-16236730 | None:intergenic |
| AACGGAATGGCCAATGCGGA+TGG | 0.542673 | 4.1:+16236720 | MS.gene34925:CDS |
| AAGTCTTCCCTCACGAGCAT+AGG | 0.565718 | 4.1:-16236045 | None:intergenic |
| CGAGGGAGACCTTGTTGCCA+CGG | 0.566086 | 4.1:+16236001 | MS.gene34925:CDS |
| TCGCTAACCACAAATCGAAT+CGG | 0.569838 | 4.1:-16235981 | None:intergenic |
| GCCAGAGACAACCAACGGAA+TGG | 0.577142 | 4.1:+16236707 | MS.gene34925:CDS |
| CTGGACACCATTGGATCACA+CGG | 0.585651 | 4.1:+16236654 | MS.gene34925:CDS |
| AAGACTTCCTATCTTAGGAA+AGG | 0.603187 | 4.1:+16236061 | MS.gene34925:CDS |
| CTCAAATCCTATGCTCGTGA+GGG | 0.605690 | 4.1:+16236038 | MS.gene34925:CDS |
| TCTCAAATCCTATGCTCGTG+AGG | 0.606550 | 4.1:+16236037 | MS.gene34925:CDS |
| GCTCTTTACTGTCAACACGT+TGG | 0.610089 | 4.1:+16236098 | MS.gene34925:CDS |
| GAAGAAGAACCAGAGCGCTA+AGG | 0.610725 | 4.1:+16235911 | MS.gene34925:CDS |
| ATTCGATTTGTGGTTAGCGA+GGG | 0.615327 | 4.1:+16235984 | MS.gene34925:CDS |
| GATTCGATTTGTGGTTAGCG+AGG | 0.620699 | 4.1:+16235983 | MS.gene34925:CDS |
| TATCAATAACCGTGGCAACA+AGG | 0.624010 | 4.1:-16236010 | None:intergenic |
| AATGGCCAATGCGGATGGAG+CGG | 0.628022 | 4.1:+16236725 | MS.gene34925:CDS |
| AACCAACGGAATGGCCAATG+CGG | 0.659288 | 4.1:+16236716 | MS.gene34925:CDS |
| TGTGATCCAATGGTGTCCAG+TGG | 0.669646 | 4.1:-16236651 | None:intergenic |
| TCTTCAAGCTCCCATTCCTG+CGG | 0.695043 | 4.1:-16236761 | None:intergenic |
| GAGAGCAGTATCAATAACCG+TGG | 0.708477 | 4.1:-16236018 | None:intergenic |
| AAGAAGCCAGAGACAACCAA+CGG | 0.709491 | 4.1:+16236702 | MS.gene34925:CDS |
| GACACCATTGGATCACACGG+AGG | 0.754267 | 4.1:+16236657 | MS.gene34925:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATCTATTTATGTTTATG+AGG | + | chr4.1:16236491-16236510 | MS.gene34925:intron | 15.0% |
| !! | TTACATTATGATGTTAAATT+GGG | + | chr4.1:16236553-16236572 | MS.gene34925:intron | 15.0% |
| !! | AAAAATACGTTTGAATTCAA+TGG | - | chr4.1:16236446-16236465 | None:intergenic | 20.0% |
| !! | ATAATATTGTAACCTCAATT+AGG | + | chr4.1:16236244-16236263 | MS.gene34925:intron | 20.0% |
| !! | CTTACATTATGATGTTAAAT+TGG | + | chr4.1:16236552-16236571 | MS.gene34925:intron | 20.0% |
| !! | TAATCAAAACTTTGCATAAA+AGG | - | chr4.1:16236597-16236616 | None:intergenic | 20.0% |
| !!! | CAATTTTCATTTTGTTGAAT+TGG | + | chr4.1:16236165-16236184 | MS.gene34925:intron | 20.0% |
| !!! | TTCAAACGTATTTTTAGTAA+TGG | + | chr4.1:16236451-16236470 | MS.gene34925:intron | 20.0% |
| ! | ATTATGATGTTAAATTGGGT+TGG | + | chr4.1:16236557-16236576 | MS.gene34925:intron | 25.0% |
| ! | ATTCAATAACGAAAACATCA+TGG | - | chr4.1:16236312-16236331 | None:intergenic | 25.0% |
| ! | TTATGAGGAATTTCATGTTT+CGG | + | chr4.1:16236506-16236525 | MS.gene34925:intron | 25.0% |
| ! | TTCAATAACGAAAACATCAT+GGG | - | chr4.1:16236311-16236330 | None:intergenic | 25.0% |
| !! | GATGTTTTCGTTATTGAATT+GGG | + | chr4.1:16236313-16236332 | MS.gene34925:intron | 25.0% |
| !! | GTTCTTTTCATTGTTGAATT+TGG | + | chr4.1:16236193-16236212 | MS.gene34925:intron | 25.0% |
| !! | TGATGTTTTCGTTATTGAAT+TGG | + | chr4.1:16236312-16236331 | MS.gene34925:intron | 25.0% |
| !! | TTGATCAACATCAATGTTTT+GGG | + | chr4.1:16235948-16235967 | MS.gene34925:CDS | 25.0% |
| !!! | GATTAGTGTCTTTTATTTTG+TGG | + | chr4.1:16236612-16236631 | MS.gene34925:intron | 25.0% |
| AACAAAACCAAACCTAATTG+AGG | - | chr4.1:16236259-16236278 | None:intergenic | 30.0% | |
| ATTGTAACCTCAATTAGGTT+TGG | + | chr4.1:16236249-16236268 | MS.gene34925:intron | 30.0% | |
| GAATTGGGTAGGAAAAAATT+TGG | + | chr4.1:16236328-16236347 | MS.gene34925:intron | 30.0% | |
| TATGAGGAATTTCATGTTTC+GGG | + | chr4.1:16236507-16236526 | MS.gene34925:intron | 30.0% | |
| ! | GTTGATCAACATCAATGTTT+TGG | + | chr4.1:16235947-16235966 | MS.gene34925:CDS | 30.0% |
| ! | TTTATAACCACACTTGCTTT+TGG | + | chr4.1:16236380-16236399 | MS.gene34925:intron | 30.0% |
| ! | TTTTCGTTATTGAATTGGGT+AGG | + | chr4.1:16236317-16236336 | MS.gene34925:intron | 30.0% |
| !!! | GCTTTTGGTTTTGTTTGTTA+TGG | + | chr4.1:16236395-16236414 | MS.gene34925:intron | 30.0% |
| AACAAAACCAAAAGCAAGTG+TGG | - | chr4.1:16236390-16236409 | None:intergenic | 35.0% | |
| AAGACTTCCTATCTTAGGAA+AGG | + | chr4.1:16236061-16236080 | MS.gene34925:CDS | 35.0% | |
| ATGAGGAATTTCATGTTTCG+GGG | + | chr4.1:16236508-16236527 | MS.gene34925:intron | 35.0% | |
| GTAACATCCTTTCCTAAGAT+AGG | - | chr4.1:16236071-16236090 | None:intergenic | 35.0% | |
| TATCAATAACCGTGGCAACA+AGG | - | chr4.1:16236013-16236032 | None:intergenic | 40.0% | |
| TCGCTAACCACAAATCGAAT+CGG | - | chr4.1:16235984-16236003 | None:intergenic | 40.0% | |
| ! | ATTCGATTTGTGGTTAGCGA+GGG | + | chr4.1:16235984-16236003 | MS.gene34925:CDS | 40.0% |
| AAGAAGCCAGAGACAACCAA+CGG | + | chr4.1:16236702-16236721 | MS.gene34925:CDS | 45.0% | |
| CTCAAATCCTATGCTCGTGA+GGG | + | chr4.1:16236038-16236057 | MS.gene34925:CDS | 45.0% | |
| GAGAGCAGTATCAATAACCG+TGG | - | chr4.1:16236021-16236040 | None:intergenic | 45.0% | |
| GAGGGAAGACTTCCTATCTT+AGG | + | chr4.1:16236056-16236075 | MS.gene34925:CDS | 45.0% | |
| GCTCTTTACTGTCAACACGT+TGG | + | chr4.1:16236098-16236117 | MS.gene34925:CDS | 45.0% | |
| GGAATGGGAGCTTGAAGAAT+TGG | + | chr4.1:16236766-16236785 | MS.gene34925:CDS | 45.0% | |
| TCTCAAATCCTATGCTCGTG+AGG | + | chr4.1:16236037-16236056 | MS.gene34925:CDS | 45.0% | |
| TGTCAACACGTTGGATCTGA+TGG | + | chr4.1:16236107-16236126 | MS.gene34925:CDS | 45.0% | |
| ! | GATTCGATTTGTGGTTAGCG+AGG | + | chr4.1:16235983-16236002 | MS.gene34925:CDS | 45.0% |
| !!! | ATCAATGTTTTGGGAAGCGC+TGG | + | chr4.1:16235957-16235976 | MS.gene34925:CDS | 45.0% |
| AACCAACGGAATGGCCAATG+CGG | + | chr4.1:16236716-16236735 | MS.gene34925:CDS | 50.0% | |
| AAGTCTTCCCTCACGAGCAT+AGG | - | chr4.1:16236048-16236067 | None:intergenic | 50.0% | |
| CTGGACACCATTGGATCACA+CGG | + | chr4.1:16236654-16236673 | MS.gene34925:CDS | 50.0% | |
| TCTTCAAGCTCCCATTCCTG+CGG | - | chr4.1:16236764-16236783 | None:intergenic | 50.0% | |
| TGTGATCCAATGGTGTCCAG+TGG | - | chr4.1:16236654-16236673 | None:intergenic | 50.0% | |
| ! | GAAGAAGAACCAGAGCGCTA+AGG | + | chr4.1:16235911-16235930 | MS.gene34925:CDS | 50.0% |
| !!! | TTTTGCAGCTCTGAGTCCAC+TGG | + | chr4.1:16236635-16236654 | MS.gene34925:intron | 50.0% |
| AACGGAATGGCCAATGCGGA+TGG | + | chr4.1:16236720-16236739 | MS.gene34925:CDS | 55.0% | |
| AATGGCCAATGCGGATGGAG+CGG | + | chr4.1:16236725-16236744 | MS.gene34925:CDS | 55.0% | |
| AGCTCCTCCGTGTGATCCAA+TGG | - | chr4.1:16236664-16236683 | None:intergenic | 55.0% | |
| CTGAGTCCACTGGACACCAT+TGG | + | chr4.1:16236645-16236664 | MS.gene34925:CDS | 55.0% | |
| GACACCATTGGATCACACGG+AGG | + | chr4.1:16236657-16236676 | MS.gene34925:CDS | 55.0% | |
| GCCAGAGACAACCAACGGAA+TGG | + | chr4.1:16236707-16236726 | MS.gene34925:CDS | 55.0% | |
| TTCTCGCATCCTTAGCGCTC+TGG | - | chr4.1:16235923-16235942 | None:intergenic | 55.0% | |
| ! | ATCCGCATTGGCCATTCCGT+TGG | - | chr4.1:16236721-16236740 | None:intergenic | 55.0% |
| ! | GCCATTCCGTTGGTTGTCTC+TGG | - | chr4.1:16236711-16236730 | None:intergenic | 55.0% |
| !! | CGCTGGACCGATTCGATTTG+TGG | + | chr4.1:16235974-16235993 | MS.gene34925:CDS | 55.0% |
| CGAGGGAGACCTTGTTGCCA+CGG | + | chr4.1:16236001-16236020 | MS.gene34925:CDS | 60.0% | |
| !! | CATTGGCTCGCCGCAGGAAT+GGG | + | chr4.1:16236751-16236770 | MS.gene34925:CDS | 60.0% |
| TGCGACCGCTCCATCCGCAT+TGG | - | chr4.1:16236733-16236752 | None:intergenic | 65.0% | |
| TGCGGATGGAGCGGTCGCAT+TGG | + | chr4.1:16236734-16236753 | MS.gene34925:CDS | 65.0% | |
| !! | GCATTGGCTCGCCGCAGGAA+TGG | + | chr4.1:16236750-16236769 | MS.gene34925:CDS | 65.0% |
| !! | CGGTCGCATTGGCTCGCCGC+AGG | + | chr4.1:16236745-16236764 | MS.gene34925:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 16235894 | 16236830 | 16235894 | ID=MS.gene34925 |
| chr4.1 | mRNA | 16235894 | 16236830 | 16235894 | ID=MS.gene34925.t1;Parent=MS.gene34925 |
| chr4.1 | exon | 16235894 | 16236128 | 16235894 | ID=MS.gene34925.t1.exon1;Parent=MS.gene34925.t1 |
| chr4.1 | CDS | 16235894 | 16236128 | 16235894 | ID=cds.MS.gene34925.t1;Parent=MS.gene34925.t1 |
| chr4.1 | exon | 16236643 | 16236830 | 16236643 | ID=MS.gene34925.t1.exon2;Parent=MS.gene34925.t1 |
| chr4.1 | CDS | 16236643 | 16236830 | 16236643 | ID=cds.MS.gene34925.t1;Parent=MS.gene34925.t1 |
| Gene Sequence |
| Protein sequence |
