AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37236


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37236.t1 MTR_6g015885 98.286 175 3 0 1 175 1 175 2.33e-119 350
MS.gene37236.t1 MTR_6g015885 98.286 175 3 0 1 175 59 233 6.00e-119 350
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37236.t1 AT5G15810 57.143 175 55 1 1 175 1 155 5.41e-55 183
MS.gene37236.t1 AT3G02320 57.471 174 54 2 1 174 1 154 3.48e-52 175

Find 37 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGTCTGCTTCTGGTTTA+AGG 0.077465 6.3:+10836568 MS.gene37236:CDS
CAAAACAATGGCTACAAATT+TGG 0.171265 6.3:+10835271 None:intergenic
CCATTTGACTTATGATCTTC+TGG 0.209624 6.3:-10836212 None:intergenic
CTTGTCTGCTTCTGGTTTAA+GGG 0.240651 6.3:+10836569 MS.gene37236:CDS
ATATAGGCCTTGTCTGCTTC+TGG 0.341486 6.3:+10836561 MS.gene37236:intron
TGAAAATGATTCTTCTGAAA+TGG 0.384979 6.3:+10836160 MS.gene37236:CDS
AGATATGCTCGTGAAATAGA+AGG 0.389227 6.3:+10836597 MS.gene37236:CDS
CTCGGTGGCTCCAGCTCTGC+CGG 0.390513 6.3:-10836359 None:intergenic
CCAGAAGATCATAAGTCAAA+TGG 0.427035 6.3:+10836212 MS.gene37236:CDS
GATTGGTCAGGTTGTTGCTC+TGG 0.436460 6.3:+10836620 MS.gene37236:CDS
GTTGCTCTGGACAACGATCC+AGG 0.448540 6.3:+10836633 MS.gene37236:CDS
TGAAGAGCAAGTTGATGATT+CGG 0.448596 6.3:+10836325 MS.gene37236:CDS
TGAAGAATATTTGTTGAAGA+AGG 0.452680 6.3:+10836115 MS.gene37236:CDS
TTAGCGGAAGAGACATCTCC+TGG 0.459743 6.3:+10836245 MS.gene37236:CDS
TATTTGTTGAAGAAGGCAAA+AGG 0.464282 6.3:+10836122 MS.gene37236:CDS
TGAGGGCATTTATATCGAAA+AGG 0.511483 6.3:+10836081 MS.gene37236:CDS
ATTTGTTGAAGAAGGCAAAA+GGG 0.517312 6.3:+10836123 MS.gene37236:CDS
TGAAATAGAAGGGATTGGTC+AGG 0.527480 6.3:+10836608 MS.gene37236:CDS
TGATTCTTCTGAAATGGTTG+TGG 0.527746 6.3:+10836166 MS.gene37236:CDS
TTTGACTTATGATCTTCTGG+TGG 0.528551 6.3:-10836209 None:intergenic
ATTGTAGTGCATGATTCTCC+AGG 0.531662 6.3:-10836263 None:intergenic
GCTCGTGAAATAGAAGGGAT+TGG 0.551513 6.3:+10836603 MS.gene37236:CDS
AAATGGAAAATGTGAGTTAG+CGG 0.567232 6.3:+10836229 MS.gene37236:CDS
GGAGCCACCGAGGGTTCTTG+AGG 0.571951 6.3:+10836370 MS.gene37236:CDS
CCTTAAACCAGAAGCAGACA+AGG 0.574066 6.3:-10836568 None:intergenic
AACGGAACCTCAAGAACCCT+CGG 0.579021 6.3:-10836377 None:intergenic
TGGAGAATCATGCACTACAA+TGG 0.582816 6.3:+10836265 MS.gene37236:CDS
AAATGCCCTCAAAACAGCAA+TGG 0.583692 6.3:-10836069 None:intergenic
GAATCATGCACTACAATGGA+AGG 0.591686 6.3:+10836269 MS.gene37236:CDS
TTTGTTGAAGAAGGCAAAAG+GGG 0.591696 6.3:+10836124 MS.gene37236:CDS
GGCAGAGCTGGAGCCACCGA+GGG 0.604326 6.3:+10836361 MS.gene37236:CDS
GATATGCTCGTGAAATAGAA+GGG 0.606358 6.3:+10836598 MS.gene37236:CDS
TTCTTCTGAAATGGTTGTGG+AGG 0.613449 6.3:+10836169 MS.gene37236:CDS
GAGCAAGTTGATGATTCGGA+AGG 0.620607 6.3:+10836329 MS.gene37236:CDS
GTCAAGATCGATGAAGAATG+TGG 0.653077 6.3:+10836296 MS.gene37236:CDS
CGGCAGAGCTGGAGCCACCG+AGG 0.657470 6.3:+10836360 MS.gene37236:CDS
GGAACCTCAAGAACCCTCGG+TGG 0.759107 6.3:-10836374 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGAATTAAAATTCAATT+AGG - chr6.3:10835605-10835624 None:intergenic 10.0%
!! AAAGAATTAAAATTCAATTA+GGG - chr6.3:10835604-10835623 None:intergenic 10.0%
!! AAAAATCTAAACTTTCAATT+AGG - chr6.3:10835459-10835478 None:intergenic 15.0%
!! AAAATCTAAACTTTCAATTA+GGG - chr6.3:10835458-10835477 None:intergenic 15.0%
!! AACATAAATGAGTTATATAA+AGG - chr6.3:10836418-10836437 None:intergenic 15.0%
!! TTTATGATGAAAAAAATGAT+GGG + chr6.3:10835640-10835659 MS.gene37236:intron 15.0%
!! AAAACAATTATACAAAACTG+AGG - chr6.3:10835954-10835973 None:intergenic 20.0%
!! AAAATTGAAACTTGAGTAAT+GGG + chr6.3:10835527-10835546 MS.gene37236:intron 20.0%
!! AAATAAGTGCATGTTTATAT+TGG - chr6.3:10835910-10835929 None:intergenic 20.0%
!! ACAAAATTGAAACTTGAATA+TGG + chr6.3:10835669-10835688 MS.gene37236:intron 20.0%
!! CAAAATTGAAACTTGAATAT+GGG + chr6.3:10835670-10835689 MS.gene37236:intron 20.0%
!! CAAATCCAATATAACAAAAA+GGG - chr6.3:10835406-10835425 None:intergenic 20.0%
!! GTTTATGATGAAAAAAATGA+TGG + chr6.3:10835639-10835658 MS.gene37236:intron 20.0%
!! TCAAATCCAATATAACAAAA+AGG - chr6.3:10835407-10835426 None:intergenic 20.0%
!!! ATTCTTTTTAATCCAATGTT+TGG + chr6.3:10835617-10835636 MS.gene37236:intron 20.0%
!!! CAGTTTTGTATAATTGTTTT+TGG + chr6.3:10835954-10835973 MS.gene37236:intron 20.0%
!!! TTTATCCCTTTTTGTTATAT+TGG + chr6.3:10835398-10835417 MS.gene37236:intron 20.0%
! AAATTGAAACTTGAGTAATG+GGG + chr6.3:10835528-10835547 MS.gene37236:intron 25.0%
! CAAAATTGAAACTTGAGTAA+TGG + chr6.3:10835526-10835545 MS.gene37236:intron 25.0%
! CACAAAACCAGAATAAATAT+AGG - chr6.3:10836022-10836041 None:intergenic 25.0%
! GATTGAACCTATATTTATTC+TGG + chr6.3:10836012-10836031 MS.gene37236:intron 25.0%
! TATCGAATTAACAATAGTGA+TGG - chr6.3:10836453-10836472 None:intergenic 25.0%
! TTATATAAAGGACGTCTAAA+GGG - chr6.3:10836406-10836425 None:intergenic 25.0%
! TTTCATCATAAACCAAACAT+TGG - chr6.3:10835632-10835651 None:intergenic 25.0%
!! GAATTTTTGTGAAATGATTG+TGG + chr6.3:10835708-10835727 MS.gene37236:intron 25.0%
!! GATTTTACAGTTATTAAGGA+AGG + chr6.3:10835296-10835315 MS.gene37236:CDS 25.0%
!! GGATGATTTTACAGTTATTA+AGG + chr6.3:10835292-10835311 MS.gene37236:CDS 25.0%
!! GGATTTGAAATTTGAAATCA+GGG + chr6.3:10835419-10835438 MS.gene37236:intron 25.0%
!! GTTTATATTGGAAGTGATTT+TGG - chr6.3:10835898-10835917 None:intergenic 25.0%
!! TGAAAATGATTCTTCTGAAA+TGG + chr6.3:10836160-10836179 MS.gene37236:CDS 25.0%
!! TGAAGAATATTTGTTGAAGA+AGG + chr6.3:10836115-10836134 MS.gene37236:CDS 25.0%
!! TGGATTTGAAATTTGAAATC+AGG + chr6.3:10835418-10835437 MS.gene37236:intron 25.0%
!! TGGTGATTTTGAATGTTAAT+TGG + chr6.3:10835736-10835755 MS.gene37236:intron 25.0%
!!! TATTAAGTAGTTTTGTGGTT+AGG + chr6.3:10835761-10835780 MS.gene37236:intron 25.0%
!!! TCTTTTATTTGTCTGCATAT+AGG + chr6.3:10836545-10836564 MS.gene37236:intron 25.0%
!!! TGATTTTCAGGTTAATAACA+GGG + chr6.3:10836040-10836059 MS.gene37236:intron 25.0%
!!! TGGATTATTAAGTAGTTTTG+TGG + chr6.3:10835756-10835775 MS.gene37236:intron 25.0%
AAATGGAAAATGTGAGTTAG+CGG + chr6.3:10836229-10836248 MS.gene37236:CDS 30.0%
ACTTTGAATTTGACCAAATC+AGG + chr6.3:10835830-10835849 MS.gene37236:intron 30.0%
CTTTGAATTTGACCAAATCA+GGG + chr6.3:10835831-10835850 MS.gene37236:intron 30.0%
GAGAGCAAATAAGTTAAATC+AGG - chr6.3:10836476-10836495 None:intergenic 30.0%
GTGAAATGATTGTGGTTTAT+TGG + chr6.3:10835716-10835735 MS.gene37236:intron 30.0%
GTTATATAAAGGACGTCTAA+AGG - chr6.3:10836407-10836426 None:intergenic 30.0%
TGGTTTATGGTGAAAGAAAA+TGG + chr6.3:10835492-10835511 MS.gene37236:intron 30.0%
TTATGGTGAAAGAAAATGGT+GGG + chr6.3:10835496-10835515 MS.gene37236:intron 30.0%
TTTATGGTGAAAGAAAATGG+TGG + chr6.3:10835495-10835514 MS.gene37236:intron 30.0%
! ATTTGTTGAAGAAGGCAAAA+GGG + chr6.3:10836123-10836142 MS.gene37236:CDS 30.0%
! TATTTGTTGAAGAAGGCAAA+AGG + chr6.3:10836122-10836141 MS.gene37236:CDS 30.0%
!! GAAATTTGGCTTTGAAATCA+TGG + chr6.3:10835565-10835584 MS.gene37236:intron 30.0%
!!! AGTCTTTTTCAATAAAGCTC+AGG + chr6.3:10835352-10835371 MS.gene37236:CDS 30.0%
!!! ATTCTGGTTTTGTGATTTTC+AGG + chr6.3:10836028-10836047 MS.gene37236:intron 30.0%
!!! GATTTTTAGTGTCTGATGTT+TGG + chr6.3:10835472-10835491 MS.gene37236:intron 30.0%
!!! GTGATTTTCAGGTTAATAAC+AGG + chr6.3:10836039-10836058 MS.gene37236:intron 30.0%
AGATATGCTCGTGAAATAGA+AGG + chr6.3:10836597-10836616 MS.gene37236:CDS 35.0%
CATTCCAAACATATCCTAAG+TGG - chr6.3:10835810-10835829 None:intergenic 35.0%
CCAGAAGATCATAAGTCAAA+TGG + chr6.3:10836212-10836231 MS.gene37236:CDS 35.0%
CTTTCCACTTAGGATATGTT+TGG + chr6.3:10835803-10835822 MS.gene37236:intron 35.0%
GATACAATCACTTTCCACTT+AGG + chr6.3:10835793-10835812 MS.gene37236:intron 35.0%
GATATGCTCGTGAAATAGAA+GGG + chr6.3:10836598-10836617 MS.gene37236:CDS 35.0%
TTTGTTGAAGAAGGCAAAAG+GGG + chr6.3:10836124-10836143 MS.gene37236:CDS 35.0%
! AGTGTCTGATGTTTGGTTTA+TGG + chr6.3:10835479-10835498 MS.gene37236:intron 35.0%
! CCATTTGACTTATGATCTTC+TGG - chr6.3:10836215-10836234 None:intergenic 35.0%
! TTTGACTTATGATCTTCTGG+TGG - chr6.3:10836212-10836231 None:intergenic 35.0%
!! GTGGCAATGTGAATTTGTTA+CGG + chr6.3:10835853-10835872 MS.gene37236:intron 35.0%
!! TGAAGAGCAAGTTGATGATT+CGG + chr6.3:10836325-10836344 MS.gene37236:CDS 35.0%
!! TGAGGGCATTTATATCGAAA+AGG + chr6.3:10836081-10836100 MS.gene37236:CDS 35.0%
!! TGATTCTTCTGAAATGGTTG+TGG + chr6.3:10836166-10836185 MS.gene37236:CDS 35.0%
!!! ATATGGGATGATGCTGATTT+TGG + chr6.3:10835686-10835705 MS.gene37236:intron 35.0%
!!! ATTTGTCCATTGCTGTTTTG+AGG + chr6.3:10836063-10836082 MS.gene37236:CDS 35.0%
!!! TGGTGCTGATTTTGGAAATT+TGG + chr6.3:10835551-10835570 MS.gene37236:intron 35.0%
!!! TTTGTCCATTGCTGTTTTGA+GGG + chr6.3:10836064-10836083 MS.gene37236:CDS 35.0%
AAATGCCCTCAAAACAGCAA+TGG - chr6.3:10836072-10836091 None:intergenic 40.0%
ATTGTAGTGCATGATTCTCC+AGG - chr6.3:10836266-10836285 None:intergenic 40.0%
GAATCATGCACTACAATGGA+AGG + chr6.3:10836269-10836288 MS.gene37236:CDS 40.0%
GTCAAGATCGATGAAGAATG+TGG + chr6.3:10836296-10836315 MS.gene37236:CDS 40.0%
TGAATTTGACCAAATCAGGG+TGG + chr6.3:10835834-10835853 MS.gene37236:intron 40.0%
TGGAGAATCATGCACTACAA+TGG + chr6.3:10836265-10836284 MS.gene37236:CDS 40.0%
! TGATTCGGAAGGAAAAAGAC+CGG + chr6.3:10836340-10836359 MS.gene37236:CDS 40.0%
! TTGAAACTTGAGTAATGGGG+TGG + chr6.3:10835531-10835550 MS.gene37236:intron 40.0%
!! CTTGTCTGCTTCTGGTTTAA+GGG + chr6.3:10836569-10836588 MS.gene37236:CDS 40.0%
!! TGAAATAGAAGGGATTGGTC+AGG + chr6.3:10836608-10836627 MS.gene37236:CDS 40.0%
!! TTCTTCTGAAATGGTTGTGG+AGG + chr6.3:10836169-10836188 MS.gene37236:CDS 40.0%
AGGACGTCTAAAGGGACAAA+CGG - chr6.3:10836398-10836417 None:intergenic 45.0%
CCTTAAACCAGAAGCAGACA+AGG - chr6.3:10836571-10836590 None:intergenic 45.0%
GCTCGTGAAATAGAAGGGAT+TGG + chr6.3:10836603-10836622 MS.gene37236:CDS 45.0%
TTCACATTGCCACCCTGATT+TGG - chr6.3:10835846-10835865 None:intergenic 45.0%
! ATATAGGCCTTGTCTGCTTC+TGG + chr6.3:10836561-10836580 MS.gene37236:intron 45.0%
!! CCTTGTCTGCTTCTGGTTTA+AGG + chr6.3:10836568-10836587 MS.gene37236:CDS 45.0%
!! GAGCAAGTTGATGATTCGGA+AGG + chr6.3:10836329-10836348 MS.gene37236:CDS 45.0%
!!! TAATGGGGTGGTGCTGATTT+TGG + chr6.3:10835543-10835562 MS.gene37236:intron 45.0%
AACGGAACCTCAAGAACCCT+CGG - chr6.3:10836380-10836399 None:intergenic 50.0%
TTAGCGGAAGAGACATCTCC+TGG + chr6.3:10836245-10836264 MS.gene37236:CDS 50.0%
! GATTGGTCAGGTTGTTGCTC+TGG + chr6.3:10836620-10836639 MS.gene37236:CDS 50.0%
AGGAAAAAGACCGGCAGAGC+TGG + chr6.3:10836349-10836368 MS.gene37236:CDS 55.0%
GGAACCTCAAGAACCCTCGG+TGG - chr6.3:10836377-10836396 None:intergenic 60.0%
GGAGCCACCGAGGGTTCTTG+AGG + chr6.3:10836370-10836389 MS.gene37236:CDS 65.0%
CTCGGTGGCTCCAGCTCTGC+CGG - chr6.3:10836362-10836381 None:intergenic 70.0%
GGCAGAGCTGGAGCCACCGA+GGG + chr6.3:10836361-10836380 MS.gene37236:CDS 70.0%
CGGCAGAGCTGGAGCCACCG+AGG + chr6.3:10836360-10836379 MS.gene37236:CDS 75.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 10835278 10836654 10835278 ID=MS.gene37236
chr6.3 mRNA 10835278 10836654 10835278 ID=MS.gene37236.t1;Parent=MS.gene37236
chr6.3 exon 10835278 10835373 10835278 ID=MS.gene37236.t1.exon1;Parent=MS.gene37236.t1
chr6.3 CDS 10835278 10835373 10835278 ID=cds.MS.gene37236.t1;Parent=MS.gene37236.t1
chr6.3 exon 10836050 10836391 10836050 ID=MS.gene37236.t1.exon2;Parent=MS.gene37236.t1
chr6.3 CDS 10836050 10836391 10836050 ID=cds.MS.gene37236.t1;Parent=MS.gene37236.t1
chr6.3 exon 10836567 10836654 10836567 ID=MS.gene37236.t1.exon3;Parent=MS.gene37236.t1
chr6.3 CDS 10836567 10836654 10836567 ID=cds.MS.gene37236.t1;Parent=MS.gene37236.t1
Gene Sequence

>MS.gene37236

ATGGCTACAAATTTGGATGATTTTACAGTTATTAAGGAAGGTGAAGCTGAAATTCTTATGAGTAAGAAAAATAAAGTCTTTTTCAATAAAGCTCAGGTAATTAATTATAAAGTTTGTTTTTTTATCCCTTTTTGTTATATTGGATTTGAAATTTGAAATCAGGGTTTTGATGAAAAACCCTAATTGAAAGTTTAGATTTTTAGTGTCTGATGTTTGGTTTATGGTGAAAGAAAATGGTGGGTTTTTGACAAAATTGAAACTTGAGTAATGGGGTGGTGCTGATTTTGGAAATTTGGCTTTGAAATCATGGTTTTGATGAAAAACCCTAATTGAATTTTAATTCTTTTTAATCCAATGTTTGGTTTATGATGAAAAAAATGATGGGCTGTGAACAAAATTGAAACTTGAATATGGGATGATGCTGATTTTGGAATTTTTGTGAAATGATTGTGGTTTATTGGTGATTTTGAATGTTAATTGGATTATTAAGTAGTTTTGTGGTTAGGTTTTTATTAGATACAATCACTTTCCACTTAGGATATGTTTGGAATGACTTTGAATTTGACCAAATCAGGGTGGCAATGTGAATTTGTTACGGCTTCAAAGTATAGTTTTTACCAAAATCACTTCCAATATAAACATGCACTTATTTTTGTTGTGCTTCTATGTAATTCCTCAGTTTTGTATAATTGTTTTTGGTGTGAATGAAAAGAAATCAACTTTTGTTTAGTGTAGATTGAACCTATATTTATTCTGGTTTTGTGATTTTCAGGTTAATAACAGGGATTTGTCCATTGCTGTTTTGAGGGCATTTATATCGAAAAGGAAGCAAGAGCATGAAGAATATTTGTTGAAGAAGGCAAAAGGGGCAAAGAAAGCATCTGAAAATGATTCTTCTGAAATGGTTGTGGAGGAAGTTGATAACAAAACTCCACCAGAAGATCATAAGTCAAATGGAAAATGTGAGTTAGCGGAAGAGACATCTCCTGGAGAATCATGCACTACAATGGAAGGTTCAGTCAAGATCGATGAAGAATGTGGTGCTGATGAAGAGCAAGTTGATGATTCGGAAGGAAAAAGACCGGCAGAGCTGGAGCCACCGAGGGTTCTTGAGGTTCCGTTTGTCCCTTTAGACGTCCTTTATATAACTCATTTATGTTATTCAAGCATCACCATCACTATTGTTAATTCGATACCTGATTTAACTTATTTGCTCTCTATACAAGTTGAATCTCAAACTAGAGTCATCAATCATGTGTATAACTGCTCTTTTATTTGTCTGCATATAGGCCTTGTCTGCTTCTGGTTTAAGGGCTCTCAGATATGCTCGTGAAATAGAAGGGATTGGTCAGGTTGTTGCTCTGGACAACGATCCAG

Protein sequence

>MS.gene37236.t1

MATNLDDFTVIKEGEAEILMSKKNKVFFNKAQVNNRDLSIAVLRAFISKRKQEHEEYLLKKAKGAKKASENDSSEMVVEEVDNKTPPEDHKSNGKCELAEETSPGESCTTMEGSVKIDEECGADEEQVDDSEGKRPAELEPPRVLEALSASGLRALRYAREIEGIGQVVALDNDP